Rw4G007820

Protein CHLOROPLAST IMPORT APPARATUS 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002359
Physical Location & Seq
Forward (+)
16839647 .. 16843681
4035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G007820.1

Sequence Viewer

Length: 1278 bp
ATGTCTTCGTGTTTTACCGGTGGCGGAAGAACCTATGCATTTGAGCTTGACATAGTCAAATCCCCTTCCACTTCAACCCGGACTTCACAAACATCCTCACCTTCTTCCACTCTCTCGGAATCAAGCAATTCGGCACTCGCAATCTCCACCCGAAAACCCCGCACCCCTCGAAAACGCCCCAACCAGACTTACAATGAAGCCGCTGCATTACTCTCCACTGCTTACCCCAACGTCTTCTCCACCAAAATCCTCACAAATCCTCGCAAATCCACCAAACCCCACGACTCCTTCCTCGACCCATCTTCCGAACTGCTCCTCCCTTTCAGACTCATCGACAATTCCGCCACTTTCCTCCTCCACAACCCCCCCATCCGACAAAAACCCAACTCTCGATTCGAGCCGAAAGCCATCAATTCATTTGATAAGTCTTGTAATTCGCCCGGGGAGTTCAATTCCCCGGTGAATTTCGACCCCACTCCCAACTCCTCCATGGAGCTCTGCGATGACTTCGACGCGGAATCTATGCTGGACGAGGAAATTGAGGAGGGTATTGATAGCATCATGGGGAGTAGCACCAGCATGAACATCGATTCCGTCGATGAGTCCACTAGCAGTGACCCAATGATGACGGACTCCTGCTATGGCTACCCAATGGGGTTGGGATTTGGCGGGAATTTCGAACTCGGGTTCGGATTCAGAAGAGGAGGAGTGAGGCCGTTGAGGCAAGTCGACGATGGAGTGAATTGGTGGAGTTTCCCGACCGTCGATGTTGTTGAAATCTCGCCCAGATTGAACAAATCTCGGCCGTCCGCTCCGGCTCCGTCGGCCCCACCTTCTGCCGGGAAGAAGAAGAAGAAGAAGATGGAGAAGCTAGCGGTGGTGGAGTCCAAGCTGGCGGAGTTGCCGCTAAAGGAGAAGAGTCCGAATCCGAATCCGAAGGAGGAGTCGGAGCCTGGGCTGATGCTGAAGCTGGACTACGACGACGTTTTGAGTGCTTGGTCCGACAAAGCGTCGCCGTTTCCGGAGGACCTGCAGGGCGGCGCTGATGGAAACGACGTCTCTGCCAGACTGGCGCAGATTGATTTATTCACGGACGCCGGTGGTTTGAGAGAGGCTAGCGTGCTCCGCTACAAGGAAAAGCGACGTACGCGCCTCTTTTCGAAGAAGATCAGGTATCAAGTCCGCAAAGTCAATGCCGATCGACGGCCAAGAATGAAGGGTAGATTTGTGAGAAGGCCAAACTCTAGCACAAATGGTCAAAGACAAAAGGAAATATGA

Protein Analysis

425

Amino Acids

47.03

Weight (kDa)

9.05

Isoelectric Point (pI)

56.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCT PF06203 370 - 412 2.4e-17 CCT motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1059
Acc36I ACCTGC 1 cut(s) 1038
AccBSI CCGCTC 1 cut(s) 812
AccI GTMKAC 1 cut(s) 729
AccII CGCG 2 cut(s) 515, 1150
AccIII TCCGGA 1 cut(s) 1021
AcoI YGGCCR 2 cut(s) 803, 1205
AcsI RAATTY 2 cut(s) 463, 673
AcuI CTGAAG 1 cut(s) 986
AcyI GRCGYC 2 cut(s) 1056, 1095
AfaI GTAC 1 cut(s) 1147
AfiI CCNNNNNNNGG 3 cut(s) 839, 1132, 1203
AgeI ACCGGT 1 cut(s) 17
AgsI TTSAA 4 cut(s) 75, 451, 776, 793
AhdI GACNNNNNGTC 1 cut(s) 1009
AjnI CCWGG 1 cut(s) 952
AjuI GAANNNNNNNTTGG 2 cut(s) 377, 409
AloI GAACNNNNNNTCC 4 cut(s) 300, 332, 575, 607
AluBI AGCT 5 cut(s) 46, 496, 871, 892, 970
AluI AGCT 5 cut(s) 46, 496, 871, 892, 970
Alw21I GWGCWC 2 cut(s) 498, 1125
Alw26I GTCTC 1 cut(s) 1063
Ama87I CYCGRG 2 cut(s) 440, 683
Aor13HI TCCGGA 1 cut(s) 1021
AoxI GGCC 5 cut(s) 713, 803, 825, 1205, 1235
ApeKI GCWGC 1 cut(s) 203
ApoI RAATTY 2 cut(s) 463, 673
ArsI GACNNNNNNTTYG 2 cut(s) 929, 961
AsiGI ACCGGT 1 cut(s) 17
AspLEI GCGC 3 cut(s) 1043, 1075, 1152
AspS9I GGNCC 3 cut(s) 826, 999, 1027
AsuC2I CCSGG 5 cut(s) 79, 441, 442, 458, 841
AsuHPI GGTGA 2 cut(s) 90, 472
AsuII TTCGAA 2 cut(s) 678, 1160
AsuNHI GCTAGC 2 cut(s) 871, 1115
AvaI CYCGRG 2 cut(s) 440, 683
AvaII GGWCC 2 cut(s) 999, 1027
BanII GRGCYC 1 cut(s) 498
BarI GAAGNNNNNNTAC 2 cut(s) 959, 991
BbsI GAAGAC 1 cut(s) 226
Bbv12I GWGCWC 2 cut(s) 498, 1125
BbvI GCAGC 1 cut(s) 190
BccI CCATC 6 cut(s) 307, 377, 416, 728, 856, 1040
BceAI ACGGC 4 cut(s) 700, 790, 1000, 1220
BcgI CGANNNNNNTGC 4 cut(s) 568, 602, 1043, 1077
BciT130I CCWGG 1 cut(s) 954
BcnI CCSGG 5 cut(s) 79, 441, 442, 458, 841
BcoDI GTCTC 1 cut(s) 1063
BfaI CTAG 4 cut(s) 609, 872, 1116, 1245
BfmI CTRYAG 1 cut(s) 1031
BfoI RGCGCY 1 cut(s) 1044
BfuAI ACCTGC 1 cut(s) 1038
BglI GCCNNNNNGGC 2 cut(s) 721, 1070
BisI GCNGC 4 cut(s) 201, 204, 905, 1039
BlsI GCNGC 4 cut(s) 202, 205, 906, 1040
Bme1390I CCNGG 6 cut(s) 79, 441, 442, 458, 841, 954
Bme18I GGWCC 2 cut(s) 999, 1027
BmeRI GACNNNNNGTC 1 cut(s) 1009
BmeT110I CYCGRG 2 cut(s) 440, 683
BmgT120I GGNCC 3 cut(s) 826, 999, 1027
BmiI GGNNCC 3 cut(s) 819, 828, 951
BmrFI CCNGG 6 cut(s) 79, 441, 442, 458, 841, 954
BmsI GCATC 2 cut(s) 567, 951
BmtI GCTAGC 2 cut(s) 875, 1119
BpiI GAAGAC 1 cut(s) 226
Bpu14I TTCGAA 2 cut(s) 678, 1160
BpuMI CCSGG 5 cut(s) 79, 441, 442, 458, 841
Bsa29I ATCGAT 1 cut(s) 588
BsaHI GRCGYC 2 cut(s) 1056, 1095
BsaJI CCNNGG 5 cut(s) 440, 441, 456, 489, 953
BsaWI WCCGGW 2 cut(s) 17, 1021
BsaXI ACNNNNNCTCC 4 cut(s) 221, 251, 300, 330
Bsc4I CCNNNNNNNGG 3 cut(s) 839, 1132, 1203
Bse118I RCCGGY 2 cut(s) 17, 1097
Bse1I ACTGG 1 cut(s) 1074
BseAI TCCGGA 1 cut(s) 1021
BseBI CCWGG 1 cut(s) 954
BseCI ATCGAT 1 cut(s) 588
BseDI CCNNGG 5 cut(s) 440, 441, 456, 489, 953
BseGI GGATG 2 cut(s) 92, 369
BseLI CCNNNNNNNGG 3 cut(s) 839, 1132, 1203
BseNI ACTGG 1 cut(s) 1074
BseRI GAGGAG 7 cut(s) 305, 344, 475, 557, 717, 720, 956
BseX3I CGGCCG 1 cut(s) 803
BseXI GCAGC 1 cut(s) 190
Bsh1236I CGCG 2 cut(s) 515, 1150
Bsh1285I CGRYCG 3 cut(s) 762, 806, 1201
BshFI GGCC 5 cut(s) 715, 805, 827, 1207, 1237
BshTI ACCGGT 1 cut(s) 17
BshVI ATCGAT 1 cut(s) 588
BsiEI CGRYCG 3 cut(s) 762, 806, 1201
BsiHKAI GWGCWC 2 cut(s) 498, 1125
BsiHKCI CYCGRG 2 cut(s) 440, 683
BsiSI CCGG 8 cut(s) 18, 79, 441, 458, 815, 840, 1022, 1098
BsiWI CGTACG 1 cut(s) 1145
BslI CCNNNNNNNGG 3 cut(s) 839, 1132, 1203
BsmAI GTCTC 1 cut(s) 1063
BsmBI CGTCTC 1 cut(s) 1063
BsnI GGCC 5 cut(s) 715, 805, 827, 1207, 1237
BsoBI CYCGRG 2 cut(s) 440, 683
Bsp119I TTCGAA 2 cut(s) 678, 1160
Bsp1286I GDGCHC 2 cut(s) 498, 1125
Bsp13I TCCGGA 1 cut(s) 1021
Bsp143I GATC 2 cut(s) 1167, 1198
Bsp19I CCATGG 1 cut(s) 489
BspANI GGCC 5 cut(s) 715, 805, 827, 1207, 1237
BspDI ATCGAT 1 cut(s) 588
BspEI TCCGGA 1 cut(s) 1021
BspFNI CGCG 2 cut(s) 515, 1150
BspLI GGNNCC 3 cut(s) 819, 828, 951
BspMAI CTGCAG 1 cut(s) 1035
BspMI ACCTGC 1 cut(s) 1038
BspOI GCTAGC 2 cut(s) 875, 1119
BspT104I TTCGAA 2 cut(s) 678, 1160
BsrBI CCGCTC 1 cut(s) 812
BsrFI RCCGGY 2 cut(s) 17, 1097
BsrI ACTGG 1 cut(s) 1074
BssAI RCCGGY 2 cut(s) 17, 1097
BssECI CCNNGG 5 cut(s) 440, 441, 456, 489, 953
BssMI GATC 2 cut(s) 1167, 1198
BssNI GRCGYC 2 cut(s) 1056, 1095
BssT1I CCWWGG 1 cut(s) 489
Bst2UI CCWGG 1 cut(s) 954
Bst4CI ACNGT 1 cut(s) 763
Bst6I CTCTTC 2 cut(s) 694, 911
BstACI GRCGYC 2 cut(s) 1056, 1095
BstBI TTCGAA 2 cut(s) 678, 1160
BstC8I GCNNGC 4 cut(s) 873, 894, 1117, 1121
BstDSI CCRYGG 1 cut(s) 489
BstF5I GGATG 2 cut(s) 92, 369
BstFNI CGCG 2 cut(s) 515, 1150
BstH2I RGCGCY 1 cut(s) 1044
BstHHI GCGC 3 cut(s) 1043, 1075, 1152
BstKTI GATC 2 cut(s) 1170, 1201
BstMAI GTCTC 1 cut(s) 1063
BstMBI GATC 2 cut(s) 1167, 1198
BstMCI CGRYCG 3 cut(s) 762, 806, 1201
BstMWI GCNNNNNNNGC 5 cut(s) 721, 824, 1070, 1125, 1147
BstNI CCWGG 1 cut(s) 954
BstSCI CCNGG 6 cut(s) 77, 439, 440, 456, 839, 952
BstSFI CTRYAG 1 cut(s) 1031
BstUI CGCG 2 cut(s) 515, 1150
BstV1I GCAGC 1 cut(s) 190
BstV2I GAAGAC 1 cut(s) 226
BstZI CGGCCG 1 cut(s) 803
Bsu15I ATCGAT 1 cut(s) 588
BsuRI GGCC 5 cut(s) 715, 805, 827, 1207, 1237
BsuTUI ATCGAT 1 cut(s) 588
BtgI CCRYGG 1 cut(s) 489
BtgZI GCGATG 1 cut(s) 516
BtsCI GGATG 2 cut(s) 92, 369
BtsI GCAGTG 2 cut(s) 216, 619
BtsIMutI CAGTG 2 cut(s) 216, 619
BveI ACCTGC 1 cut(s) 1038
Cac8I GCNNGC 4 cut(s) 873, 894, 1117, 1121
CfoI GCGC 3 cut(s) 1043, 1075, 1152
Cfr10I RCCGGY 2 cut(s) 17, 1097
Cfr13I GGNCC 3 cut(s) 826, 999, 1027
Cfr9I CCCGGG 1 cut(s) 440
ClaI ATCGAT 1 cut(s) 588
CseI GACGC 3 cut(s) 521, 999, 1103
Csp6I GTAC 1 cut(s) 1146
CspAI ACCGGT 1 cut(s) 17
CviAII CATG 3 cut(s) 490, 562, 580
CviQI GTAC 1 cut(s) 1146
DpnI GATC 2 cut(s) 1169, 1200
DpnII GATC 2 cut(s) 1167, 1198
DriI GACNNNNNGTC 1 cut(s) 1009
EaeI YGGCCR 2 cut(s) 803, 1205
EagI CGGCCG 1 cut(s) 803
Eam1104I CTCTTC 2 cut(s) 694, 911
Eam1105I GACNNNNNGTC 1 cut(s) 1009
EarI CTCTTC 2 cut(s) 694, 911
EciI GGCGGA 3 cut(s) 39, 331, 911
Ecl136II GAGCTC 1 cut(s) 496
EclXI CGGCCG 1 cut(s) 803
Eco130I CCWWGG 1 cut(s) 489
Eco24I GRGCYC 1 cut(s) 498
Eco47I GGWCC 2 cut(s) 999, 1027
Eco52I CGGCCG 1 cut(s) 803
Eco53kI GAGCTC 1 cut(s) 496
Eco57I CTGAAG 1 cut(s) 986
Eco88I CYCGRG 2 cut(s) 440, 683
EcoICRI GAGCTC 1 cut(s) 496
EcoO109I RGGNCCY 1 cut(s) 1027
EcoRII CCWGG 1 cut(s) 952
EcoT14I CCWWGG 1 cut(s) 489
EcoT22I ATGCAT 1 cut(s) 40
EcoT38I GRGCYC 1 cut(s) 498
ErhI CCWWGG 1 cut(s) 489
Esp3I CGTCTC 1 cut(s) 1063
FaeI CATG 3 cut(s) 493, 565, 583
FaiI YATR 8 cut(s) 36, 53, 491, 524, 563, 581, 642, 1276
FatI CATG 3 cut(s) 489, 561, 579
FauI CCCGC 2 cut(s) 167, 662
FblI GTMKAC 1 cut(s) 729
Fnu4HI GCNGC 4 cut(s) 201, 204, 905, 1039
FokI GGATG 2 cut(s) 79, 356
FriOI GRGCYC 1 cut(s) 498
Fsp4HI GCNGC 4 cut(s) 201, 204, 905, 1039
FspBI CTAG 4 cut(s) 609, 872, 1116, 1245
GlaI GCGC 3 cut(s) 1042, 1074, 1151
GluI GCNGC 4 cut(s) 201, 204, 905, 1039
HaeII RGCGCY 1 cut(s) 1044
HaeIII GGCC 5 cut(s) 715, 805, 827, 1207, 1237
HapII CCGG 8 cut(s) 18, 79, 441, 458, 815, 840, 1022, 1098
HgaI GACGC 3 cut(s) 521, 999, 1103
HhaI GCGC 3 cut(s) 1043, 1075, 1152
Hin1I GRCGYC 2 cut(s) 1056, 1095
Hin1II CATG 3 cut(s) 493, 565, 583
Hin6I GCGC 3 cut(s) 1041, 1073, 1150
HinP1I GCGC 3 cut(s) 1041, 1073, 1150
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HpaII CCGG 8 cut(s) 18, 79, 441, 458, 815, 840, 1022, 1098
HphI GGTGA 2 cut(s) 90, 472
Hpy166II GTNNAC 2 cut(s) 606, 730
Hpy188III TCNNGA 3 cut(s) 390, 757, 1022
Hpy8I GTNNAC 2 cut(s) 606, 730
HpyAV CCTTC 7 cut(s) 75, 111, 298, 843, 931, 1210, 1227
HpyCH4III ACNGT 1 cut(s) 763
HpyCH4IV ACGT 4 cut(s) 231, 984, 1056, 1144
HpyCH4V TGCA 3 cut(s) 38, 206, 1033
HpyF10VI GCNNNNNNNGC 5 cut(s) 721, 824, 1070, 1125, 1147
HpySE526I ACGT 4 cut(s) 231, 984, 1056, 1144
Hsp92I GRCGYC 2 cut(s) 1056, 1095
Hsp92II CATG 3 cut(s) 493, 565, 583
HspAI GCGC 3 cut(s) 1041, 1073, 1150
Kpn2I TCCGGA 1 cut(s) 1021
Kzo9I GATC 2 cut(s) 1167, 1198
LmnI GCTCC 6 cut(s) 318, 493, 817, 823, 949, 1128
Lsp1109I GCAGC 1 cut(s) 190
LweI GCATC 2 cut(s) 567, 951
MaeI CTAG 4 cut(s) 609, 872, 1116, 1245
MaeII ACGT 4 cut(s) 231, 984, 1056, 1144
MaeIII GTNAC 1 cut(s) 614
MalI GATC 2 cut(s) 1169, 1200
MbiI CCGCTC 1 cut(s) 812
MboI GATC 2 cut(s) 1167, 1198
MhlI GDGCHC 2 cut(s) 498, 1125
MluCI AATT 9 cut(s) 127, 337, 412, 433, 451, 463, 537, 673, 742
MlyI GAGTC 7 cut(s) 278, 321, 611, 626, 893, 928, 953
MmeI TCCRAC 3 cut(s) 397, 927, 1026
Mph1103I ATGCAT 1 cut(s) 40
MroI TCCGGA 1 cut(s) 1021
MslI CAYNNNNRTG 1 cut(s) 578
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 8 cut(s) 18, 79, 441, 458, 815, 840, 1022, 1098
MspR9I CCNGG 6 cut(s) 79, 441, 442, 458, 841, 954
MvaI CCWGG 1 cut(s) 954
MvnI CGCG 2 cut(s) 515, 1150
MwoI GCNNNNNNNGC 5 cut(s) 721, 824, 1070, 1125, 1147
NciI CCSGG 5 cut(s) 79, 441, 442, 458, 841
NcoI CCATGG 1 cut(s) 489
NdeII GATC 2 cut(s) 1167, 1198
NheI GCTAGC 2 cut(s) 871, 1115
NlaIII CATG 3 cut(s) 493, 565, 583
NlaIV GGNNCC 3 cut(s) 819, 828, 951
NmeAIII GCCGAG 1 cut(s) 781
NmuCI GTSAC 1 cut(s) 614
NsiI ATGCAT 1 cut(s) 40
NspV TTCGAA 2 cut(s) 678, 1160
PcsI WCGNNNNNNNCGW 1 cut(s) 594
PfeI GAWTC 7 cut(s) 119, 393, 518, 590, 693, 925, 931
Pfl23II CGTACG 1 cut(s) 1145
PflFI GACNNNGTC 1 cut(s) 53
PinAI ACCGGT 1 cut(s) 17
PkrI GCNGC 4 cut(s) 202, 205, 906, 1040
Ple19I CGATCG 1 cut(s) 1201
PleI GAGTC 7 cut(s) 278, 321, 610, 626, 892, 927, 952
PpsI GAGTC 7 cut(s) 278, 321, 610, 626, 892, 927, 952
PpuMI RGGWCCY 1 cut(s) 1027
Psp124BI GAGCTC 1 cut(s) 498
Psp5II RGGWCCY 1 cut(s) 1027
Psp6I CCWGG 1 cut(s) 952
PspGI CCWGG 1 cut(s) 952
PspLI CGTACG 1 cut(s) 1145
PspN4I GGNNCC 3 cut(s) 819, 828, 951
PspPI GGNCC 3 cut(s) 826, 999, 1027
PspPPI RGGWCCY 1 cut(s) 1027
PstI CTGCAG 1 cut(s) 1035
PsyI GACNNNGTC 1 cut(s) 53
PvuI CGATCG 1 cut(s) 1201
RsaI GTAC 1 cut(s) 1147
RsaNI GTAC 1 cut(s) 1146
RseI CAYNNNNRTG 1 cut(s) 578
SacI GAGCTC 1 cut(s) 498
SalI GTCGAC 1 cut(s) 728
SatI GCNGC 4 cut(s) 201, 204, 905, 1039
Sau3AI GATC 2 cut(s) 1167, 1198
Sau96I GGNCC 3 cut(s) 826, 999, 1027
SbfI CCTGCAGG 1 cut(s) 1035
SchI GAGTC 7 cut(s) 278, 321, 611, 626, 893, 928, 953
ScrFI CCNGG 6 cut(s) 79, 441, 442, 458, 841, 954
SdaI CCTGCAGG 1 cut(s) 1035
SduI GDGCHC 2 cut(s) 498, 1125
SfaNI GCATC 2 cut(s) 567, 951
SfcI CTRYAG 1 cut(s) 1031
SfuI TTCGAA 2 cut(s) 678, 1160
SgrAI CRCCGGYG 1 cut(s) 1097
SinI GGWCC 2 cut(s) 999, 1027
SmaI CCCGGG 1 cut(s) 442
SmiMI CAYNNNNRTG 1 cut(s) 578
Sse8387I CCTGCAGG 1 cut(s) 1035
Sse9I AATT 9 cut(s) 127, 337, 412, 433, 451, 463, 537, 673, 742
SspMI CTAG 4 cut(s) 609, 872, 1116, 1245
SstI GAGCTC 1 cut(s) 498
StyD4I CCNGG 6 cut(s) 77, 439, 440, 456, 839, 952
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 1 cut(s) 763
TaiI ACGT 4 cut(s) 234, 987, 1059, 1147
TasI AATT 9 cut(s) 127, 337, 412, 433, 451, 463, 537, 673, 742
TauI GCSGC 3 cut(s) 203, 907, 1041
TfiI GAWTC 7 cut(s) 119, 393, 518, 590, 693, 925, 931
TscAI CASTG 2 cut(s) 223, 619
TseFI GTSAC 1 cut(s) 614
TseI GCWGC 1 cut(s) 203
Tsp45I GTSAC 1 cut(s) 614
TspDTI ATGAA 4 cut(s) 210, 405, 596, 1229
TspGWI ACGGA 4 cut(s) 583, 644, 810, 1106
TspMI CCCGGG 1 cut(s) 440
TspRI CASTG 2 cut(s) 223, 619
Tth111I GACNNNGTC 1 cut(s) 53
VpaK11BI GGWCC 2 cut(s) 999, 1027
XapI RAATTY 2 cut(s) 463, 673
XcmI CCANNNNNNNNNTGG 1 cut(s) 487
XmaI CCCGGG 1 cut(s) 440
XmiI GTMKAC 1 cut(s) 729
XspI CTAG 4 cut(s) 609, 872, 1116, 1245
ZraI GACGTC 1 cut(s) 1057
Zsp2I ATGCAT 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.