pycom17g04650

vacuolar amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004035
Physical Location & Seq
Forward (+)
3212091 .. 3213981
1891 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1299 bp
ATGGATGCTGATCATTTAGAAAAATCGTCATCCTTGAGCTTGCCTCTTATTCTTGGTGAGGGGCAGCATGAGCAAGCTGGAAGCAAGGTAGTAGAAGAAGTAGAGTCAAGCTTTCATCACCAAACTACAGCCACAACCTCCTTCCTCAAAACCTGTTTCAATGGACTCAATGCACTCTCAGGGATACTGTCAGTTCCTTATGCACTAGCATCTGGAGGATGGCTAAGCTTGATCTTCCTTTTCTTAGTTGCGGCTGCAGCCTTCTACTCAGGTTTGTTAATCCAGAGATGCATGGATGTGGATTCTGACATAAGAACTTATCAGGACATAGGTGAACGCGCATTCGGGAAGAAGGGAAGAATACTGCTGTCGATTGTCATGAATGTAGATCTGCACTTGGTTGCAACGGGGTTCCTAATTTTGGAAGGAGATAACTTAAGCAATTTATTTCCTGGCGTAGAATTAGACGTGGCCGGATTAAGAATTGGCGGAGAACAATGCTTTATAATTGTTGTTGCTCTCATTATTCTGCCAACAGTTTGGTTAGATAACCTAAGCCTTCTTTCTTACGTATCTGCAAGTGGAGTTTTAGCCTCTGCCATCATCCTCGGTTCGATTTTGTGGACTGGTGCTTTTGAAGGAATTGGATTTCACGAAGAGGGCACACCACTGAAGTGGAATGGAATCCCTACGGCTATTAGCTTGTACGCGTTTTGTTACTGCGCGCATCCGGTGTTCCCGACCCTCTACACTTCAATGAAAAACAAACGTCAGTTCTCCAATGTTCTTCTCCTATGTTTCATCTTGTGCACCATCTGTTACGCATCAATGGCAGTTTTCGGGTACCTAATGTTTGGATCTACGGTTCAATCACAAATAACTTTAAATCTCCCAACTCACAAAATCAGCTCAAAAGTCGCGATATACACCACGCTGGTCAATCCCATATCCAAATATGCTTTGATGGTTACACCAGTTGTGAATGCTGCAAAGAAGAGGTTCCCGAGTCACTACAACAAGAGACTCATCGGCATGTTAGCCAGCACCACCTTGGTGACAAGCACCGTTATAGTAGCTTTGGCTATACCATTTTTCGCTTATCTCATGTCACTCGTCGGAGCATTTTTAAGTGTAACAGCTTCAATTATATTGCCGTGCTTGTGCTACCTTAAAATTTCAGGTATTTATCGACGCCTCGGATGCGAGATGTTGATTATAGGGCTTATATTACTACTGAGTGCTGCAGTTGTTGTATTCGGCACTTACACATCTCTAGTAGAAATAATAGGGCACTTGTAA

Protein Analysis

433

Amino Acids

46.75

Weight (kDa)

7.11

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 45 - 423 2.1e-58 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 506
Acc65I GGTACC 1 cut(s) 843
AccB1I GGYRCC 1 cut(s) 843
AccII CGCG 4 cut(s) 339, 710, 725, 920
AciI CCGC 2 cut(s) 251, 489
AclWI GGATC 1 cut(s) 865
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 1 cut(s) 1173
AcuI CTGAAG 1 cut(s) 692
AcyI GRCGYC 1 cut(s) 1192
AfaI GTAC 2 cut(s) 707, 845
AfiI CCNNNNNNNGG 1 cut(s) 421
AflII CTTAAG 1 cut(s) 436
AflIII ACRYGT 1 cut(s) 708
AgsI TTSAA 5 cut(s) 160, 638, 756, 869, 1143
AjiI CACGTC 1 cut(s) 469
AjnI CCWGG 1 cut(s) 451
AleI CACNNNNGTG 2 cut(s) 673, 1052
AloI GAACNNNNNNTCC 2 cut(s) 849, 881
AluBI AGCT 8 cut(s) 39, 77, 111, 228, 702, 909, 1076, 1139
AluI AGCT 8 cut(s) 39, 77, 111, 228, 702, 909, 1076, 1139
Alw21I GWGCWC 1 cut(s) 812
Alw26I GTCTC 1 cut(s) 1015
Alw44I GTGCAC 1 cut(s) 808
AlwI GGATC 1 cut(s) 865
Ama87I CYCGRG 1 cut(s) 1003
AoxI GGCC 1 cut(s) 471
ApaLI GTGCAC 1 cut(s) 808
ApeKI GCWGC 5 cut(s) 64, 254, 257, 986, 1241
ApoI RAATTY 1 cut(s) 1173
Asp700I GAANNNNTTC 1 cut(s) 998
Asp718I GGTACC 1 cut(s) 843
AspLEI GCGC 3 cut(s) 341, 725, 727
AsuHPI GGTGA 4 cut(s) 68, 110, 344, 1066
AvaI CYCGRG 1 cut(s) 1003
BaeGI GKGCMC 3 cut(s) 665, 812, 1293
BanI GGYRCC 1 cut(s) 843
Bbv12I GWGCWC 1 cut(s) 812
BbvI GCAGC 5 cut(s) 76, 241, 269, 973, 1228
BccI CCATC 4 cut(s) 213, 608, 821, 958
BceAI ACGGC 2 cut(s) 708, 1138
BciT130I CCWGG 1 cut(s) 453
BciVI GTATCC 1 cut(s) 177
BclI TGATCA 1 cut(s) 10
BcoDI GTCTC 1 cut(s) 1015
BfaI CTAG 2 cut(s) 206, 1274
BfmI CTRYAG 3 cut(s) 126, 255, 1242
BfrI CTTAAG 1 cut(s) 436
BfuI GTATCC 1 cut(s) 177
BglII AGATCT 1 cut(s) 388
BisI GCNGC 6 cut(s) 65, 252, 255, 258, 987, 1242
BlpI GCTNAGC 1 cut(s) 224
BlsI GCNGC 6 cut(s) 66, 253, 256, 259, 988, 1243
Bme1390I CCNGG 1 cut(s) 453
BmeT110I CYCGRG 1 cut(s) 1003
BmgBI CACGTC 1 cut(s) 469
BmiI GGNNCC 3 cut(s) 413, 845, 1001
BmrFI CCNGG 1 cut(s) 453
BmsI GCATC 5 cut(s) 218, 278, 736, 833, 1190
BplI GAGNNNNNCTC 2 cut(s) 28, 60
BpmI CTGGAG 1 cut(s) 234
Bpu10I CCTNAGC 1 cut(s) 554
Bpu1102I GCTNAGC 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 55
BsaAI YACGTR 1 cut(s) 571
BsaBI GATNNNNATC 1 cut(s) 9
BsaHI GRCGYC 1 cut(s) 1192
BsaJI CCNNGG 3 cut(s) 607, 1050, 1195
BsaWI WCCGGW 1 cut(s) 730
Bsc4I CCNNNNNNNGG 1 cut(s) 421
Bse1I ACTGG 2 cut(s) 631, 974
Bse8I GATNNNNATC 1 cut(s) 9
BseBI CCWGG 1 cut(s) 453
BseDI CCNNGG 3 cut(s) 607, 1050, 1195
BseGI GGATG 7 cut(s) 10, 29, 224, 301, 603, 727, 1205
BseJI GATNNNNATC 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 421
BseMII CTCAG 3 cut(s) 192, 282, 1226
BseNI ACTGG 2 cut(s) 631, 974
BsePI GCGCGC 1 cut(s) 723
BseSI GKGCMC 3 cut(s) 665, 812, 1293
BseXI GCAGC 5 cut(s) 76, 241, 269, 973, 1228
BsgI GTGCAG 1 cut(s) 377
Bsh1236I CGCG 4 cut(s) 339, 710, 725, 920
BshFI GGCC 1 cut(s) 473
BshNI GGYRCC 1 cut(s) 843
BsiHKAI GWGCWC 1 cut(s) 812
BsiHKCI CYCGRG 1 cut(s) 1003
BsiSI CCGG 2 cut(s) 474, 731
BslI CCNNNNNNNGG 1 cut(s) 421
BsmAI GTCTC 1 cut(s) 1015
BsmI GAATGC 2 cut(s) 341, 988
BsnI GGCC 1 cut(s) 473
BsoBI CYCGRG 1 cut(s) 1003
Bsp1286I GDGCHC 3 cut(s) 665, 812, 1293
Bsp143I GATC 4 cut(s) 10, 231, 388, 857
Bsp1720I GCTNAGC 1 cut(s) 224
Bsp68I TCGCGA 1 cut(s) 920
BspACI CCGC 2 cut(s) 251, 489
BspANI GGCC 1 cut(s) 473
BspCNI CTCAG 3 cut(s) 191, 281, 1227
BspFNI CGCG 4 cut(s) 339, 710, 725, 920
BspHI TCATGA 1 cut(s) 378
BspLI GGNNCC 3 cut(s) 413, 845, 1001
BspMAI CTGCAG 2 cut(s) 259, 1246
BspPI GGATC 1 cut(s) 865
BspT107I GGYRCC 1 cut(s) 843
BspTI CTTAAG 1 cut(s) 436
BsrI ACTGG 2 cut(s) 631, 974
BssECI CCNNGG 3 cut(s) 607, 1050, 1195
BssHII GCGCGC 1 cut(s) 723
BssMI GATC 4 cut(s) 10, 231, 388, 857
BssNI GRCGYC 1 cut(s) 1192
BssT1I CCWWGG 1 cut(s) 1050
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 4 cut(s) 189, 538, 865, 1066
Bst6I CTCTTC 2 cut(s) 651, 989
BstACI GRCGYC 1 cut(s) 1192
BstAFI CTTAAG 1 cut(s) 436
BstBAI YACGTR 1 cut(s) 571
BstC8I GCNNGC 4 cut(s) 41, 75, 725, 1042
BstDEI CTNAG 6 cut(s) 178, 224, 244, 268, 554, 1235
BstF5I GGATG 7 cut(s) 10, 29, 224, 301, 603, 727, 1205
BstFNI CGCG 4 cut(s) 339, 710, 725, 920
BstHHI GCGC 3 cut(s) 341, 725, 727
BstKTI GATC 4 cut(s) 13, 234, 391, 860
BstMAI GTCTC 1 cut(s) 1015
BstMBI GATC 4 cut(s) 10, 231, 388, 857
BstMWI GCNNNNNNNGC 4 cut(s) 70, 257, 830, 1200
BstNI CCWGG 1 cut(s) 453
BstNSI RCATGY 1 cut(s) 1036
BstSCI CCNGG 1 cut(s) 451
BstSFI CTRYAG 3 cut(s) 126, 255, 1242
BstSLI GKGCMC 3 cut(s) 665, 812, 1293
BstSNI TACGTA 1 cut(s) 571
BstUI CGCG 4 cut(s) 339, 710, 725, 920
BstV1I GCAGC 5 cut(s) 76, 241, 269, 973, 1228
BstX2I RGATCY 2 cut(s) 388, 857
BstXI CCANNNNNNTGG 2 cut(s) 540, 675
BstYI RGATCY 2 cut(s) 388, 857
BsuI GTATCC 1 cut(s) 177
BsuRI GGCC 1 cut(s) 473
BtrI CACGTC 1 cut(s) 469
BtsCI GGATG 7 cut(s) 10, 29, 224, 301, 603, 727, 1205
BtsIMutI CAGTG 1 cut(s) 668
BtuMI TCGCGA 1 cut(s) 920
Cac8I GCNNGC 4 cut(s) 41, 75, 725, 1042
CciI TCATGA 1 cut(s) 378
CfoI GCGC 3 cut(s) 341, 725, 727
CseI GACGC 1 cut(s) 1200
Csp6I GTAC 2 cut(s) 706, 844
CviAII CATG 5 cut(s) 68, 292, 379, 1033, 1105
CviQI GTAC 2 cut(s) 706, 844
DdeI CTNAG 6 cut(s) 178, 224, 244, 268, 554, 1235
DpnI GATC 4 cut(s) 12, 233, 390, 859
DpnII GATC 4 cut(s) 10, 231, 388, 857
DraI TTTAAA 1 cut(s) 885
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 2 cut(s) 651, 989
EarI CTCTTC 2 cut(s) 651, 989
EciI GGCGGA 1 cut(s) 504
Eco105I TACGTA 1 cut(s) 571
Eco130I CCWWGG 1 cut(s) 1050
Eco57I CTGAAG 1 cut(s) 692
Eco88I CYCGRG 1 cut(s) 1003
EcoRII CCWGG 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 1050
EcoT22I ATGCAT 1 cut(s) 293
ErhI CCWWGG 1 cut(s) 1050
FaeI CATG 5 cut(s) 71, 295, 382, 1036, 1108
FatI CATG 5 cut(s) 67, 291, 378, 1032, 1104
FbaI TGATCA 1 cut(s) 10
Fnu4HI GCNGC 6 cut(s) 65, 252, 255, 258, 987, 1242
FokI GGATG 7 cut(s) 16, 17, 231, 308, 590, 714, 1212
Fsp4HI GCNGC 6 cut(s) 65, 252, 255, 258, 987, 1242
FspBI CTAG 2 cut(s) 206, 1274
GlaI GCGC 3 cut(s) 340, 724, 726
GluI GCNGC 6 cut(s) 65, 252, 255, 258, 987, 1242
GsuI CTGGAG 1 cut(s) 234
HaeIII GGCC 1 cut(s) 473
HapII CCGG 2 cut(s) 474, 731
HgaI GACGC 1 cut(s) 1200
HhaI GCGC 3 cut(s) 341, 725, 727
Hin1I GRCGYC 1 cut(s) 1192
Hin1II CATG 5 cut(s) 71, 295, 382, 1036, 1108
Hin6I GCGC 3 cut(s) 339, 723, 725
HinP1I GCGC 3 cut(s) 339, 723, 725
HindIII AAGCTT 2 cut(s) 109, 226
HinfI GANTC 6 cut(s) 104, 165, 302, 684, 1006, 1023
HpaII CCGG 2 cut(s) 474, 731
HphI GGTGA 4 cut(s) 68, 110, 344, 1066
Hpy166II GTNNAC 3 cut(s) 335, 624, 810
Hpy188I TCNGA 3 cut(s) 307, 1118, 1199
Hpy188III TCNNGA 9 cut(s) 213, 283, 323, 346, 379, 653, 739, 919, 1003
Hpy8I GTNNAC 3 cut(s) 335, 624, 810
Hpy99I CGWCG 2 cut(s) 1118, 1194
HpyAV CCTTC 6 cut(s) 151, 271, 346, 419, 569, 632
HpyCH4III ACNGT 4 cut(s) 189, 538, 865, 1066
HpyCH4IV ACGT 3 cut(s) 468, 570, 769
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 257, 830, 1200
HpyF3I CTNAG 6 cut(s) 178, 224, 244, 268, 554, 1235
HpySE526I ACGT 3 cut(s) 468, 570, 769
Hsp92I GRCGYC 1 cut(s) 1192
Hsp92II CATG 5 cut(s) 71, 295, 382, 1036, 1108
HspAI GCGC 3 cut(s) 339, 723, 725
KpnI GGTACC 1 cut(s) 847
Ksp22I TGATCA 1 cut(s) 10
Kzo9I GATC 4 cut(s) 10, 231, 388, 857
LmnI GCTCC 1 cut(s) 1118
Lsp1109I GCAGC 5 cut(s) 76, 241, 269, 973, 1228
LweI GCATC 5 cut(s) 218, 278, 736, 833, 1190
MaeI CTAG 2 cut(s) 206, 1274
MaeII ACGT 3 cut(s) 468, 570, 769
MaeIII GTNAC 7 cut(s) 716, 818, 967, 1007, 1054, 1107, 1132
MalI GATC 4 cut(s) 12, 233, 390, 859
MboI GATC 4 cut(s) 10, 231, 388, 857
MboII GAAGA 7 cut(s) 107, 226, 361, 369, 668, 779, 1006
MflI RGATCY 2 cut(s) 388, 857
MhlI GDGCHC 3 cut(s) 665, 812, 1293
MluCI AATT 8 cut(s) 417, 442, 461, 483, 507, 642, 1143, 1173
MluI ACGCGT 1 cut(s) 708
MlyI GAGTC 4 cut(s) 113, 159, 1015, 1017
MmeI TCCRAC 1 cut(s) 1096
Mph1103I ATGCAT 1 cut(s) 293
MroXI GAANNNNTTC 1 cut(s) 998
MseI TTAA 6 cut(s) 278, 437, 479, 884, 1127, 1170
MslI CAYNNNNRTG 5 cut(s) 296, 673, 755, 1031, 1052
MspCI CTTAAG 1 cut(s) 436
MspI CCGG 2 cut(s) 474, 731
MspR9I CCNGG 1 cut(s) 453
Mva1269I GAATGC 2 cut(s) 341, 988
MvaI CCWGG 1 cut(s) 453
MvnI CGCG 4 cut(s) 339, 710, 725, 920
MwoI GCNNNNNNNGC 4 cut(s) 70, 257, 830, 1200
NdeII GATC 4 cut(s) 10, 231, 388, 857
NlaIII CATG 5 cut(s) 71, 295, 382, 1036, 1108
NlaIV GGNNCC 3 cut(s) 413, 845, 1001
NmuCI GTSAC 3 cut(s) 1007, 1054, 1107
NruI TCGCGA 1 cut(s) 920
NsiI ATGCAT 1 cut(s) 293
NspI RCATGY 1 cut(s) 1036
OliI CACNNNNGTG 2 cut(s) 673, 1052
PagI TCATGA 1 cut(s) 378
PauI GCGCGC 1 cut(s) 723
PctI GAATGC 2 cut(s) 341, 988
PdmI GAANNNNTTC 1 cut(s) 998
PfeI GAWTC 2 cut(s) 302, 684
PkrI GCNGC 6 cut(s) 66, 253, 256, 259, 988, 1243
PleI GAGTC 4 cut(s) 112, 159, 1014, 1017
PpsI GAGTC 4 cut(s) 112, 159, 1014, 1017
Ppu21I YACGTR 1 cut(s) 571
PsiI TTATAA 1 cut(s) 506
Psp6I CCWGG 1 cut(s) 451
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 3 cut(s) 413, 845, 1001
PstI CTGCAG 2 cut(s) 259, 1246
PsuI RGATCY 2 cut(s) 388, 857
PteI GCGCGC 1 cut(s) 723
RruI TCGCGA 1 cut(s) 920
RsaI GTAC 2 cut(s) 707, 845
RsaNI GTAC 2 cut(s) 706, 844
RseI CAYNNNNRTG 5 cut(s) 296, 673, 755, 1031, 1052
SaqAI TTAA 6 cut(s) 278, 437, 479, 884, 1127, 1170
SatI GCNGC 6 cut(s) 65, 252, 255, 258, 987, 1242
Sau3AI GATC 4 cut(s) 10, 231, 388, 857
SchI GAGTC 4 cut(s) 113, 159, 1015, 1017
ScrFI CCNGG 1 cut(s) 453
SduI GDGCHC 3 cut(s) 665, 812, 1293
SfaNI GCATC 5 cut(s) 218, 278, 736, 833, 1190
SfcI CTRYAG 3 cut(s) 126, 255, 1242
SmiMI CAYNNNNRTG 5 cut(s) 296, 673, 755, 1031, 1052
SmlI CTYRAG 2 cut(s) 34, 436
SmoI CTYRAG 2 cut(s) 34, 436
SnaBI TACGTA 1 cut(s) 571
Sse9I AATT 8 cut(s) 417, 442, 461, 483, 507, 642, 1143, 1173
SsiI CCGC 2 cut(s) 251, 489
SspMI CTAG 2 cut(s) 206, 1274
StyD4I CCNGG 1 cut(s) 451
StyI CCWWGG 1 cut(s) 1050
TaaI ACNGT 4 cut(s) 189, 538, 865, 1066
TaiI ACGT 3 cut(s) 471, 573, 772
TaqI TCGA 3 cut(s) 371, 614, 1189
TasI AATT 8 cut(s) 417, 442, 461, 483, 507, 642, 1143, 1173
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 2 cut(s) 302, 684
Tru1I TTAA 6 cut(s) 278, 437, 479, 884, 1127, 1170
Tru9I TTAA 6 cut(s) 278, 437, 479, 884, 1127, 1170
TscAI CASTG 1 cut(s) 675
TseFI GTSAC 3 cut(s) 1007, 1054, 1107
TseI GCWGC 5 cut(s) 64, 254, 257, 986, 1241
Tsp45I GTSAC 3 cut(s) 1007, 1054, 1107
TspDTI ATGAA 4 cut(s) 104, 395, 773, 790
TspRI CASTG 1 cut(s) 675
Vha464I CTTAAG 1 cut(s) 436
VneI GTGCAC 1 cut(s) 808
XapI RAATTY 1 cut(s) 1173
XceI RCATGY 1 cut(s) 1036
XcmI CCANNNNNNNNNTGG 1 cut(s) 1048
XmnI GAANNNNTTC 1 cut(s) 998
XspI CTAG 2 cut(s) 206, 1274
Zsp2I ATGCAT 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.