Rh2BG615900

vacuolar amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004035
Physical Location & Seq
Reverse (-)
83797740 .. 83802579
4840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG615900.1

Sequence Viewer

Length: 1008 bp
ATGGATATGGATTCTAGCATAAGAACTTATCCTGAAATAGGCGAACGCGCATTTGGGAAAAAGGGAAAAATATTGATGTCAATTTTCATGAACTTAGAACTCTACTTAGTCGCAACAGGTTTCCTGATTTTGGAAGGAGATAACTTACATAACATGTTTCCAACCATTGGATTTGAAGTGGAGGGTTTTAAAATTGGTGGGAAAGAATGCTTCATAGTGATTGCTGCCTTCATTGTTCTGCCAACTGTATGGTTAGACAACTTGAGCCTTCTTTCCTATGTATCTGCTAGTGGGGTTTTGGCTTCTGCTATCATTCTGGGATCAATTTTGTGGACTGGTGCTCTTGACGGAGTTGGATTTCATCAGAAGGGGTCACCGATTAAGTGGAGTGGAATCCCTACTGCTGTCAGCTTATACGCCTTTTGTTATTGTGCTCATCCTGTGTTCCCTACCCTGTACACTTCTATGAAAAACAAAAAACAGTTCTCCAATGTTCTCCTTGTCTGCTTTATATTATGCACCATCTGTTATGCATCAATGGCAGTTTTTGGGTACTTAATGTTCGGGTCAACAGTTCAATCGCAGATAACTCTAAACCTCCCGACTAGCAAAATTAGCTCACAAGTCGCAATATACACCACCCTGGTCAATCCAATATCCAAATTTGCTTTGATGGTTACACCAATAATGAATGCTGCAAAAAGATGGTTTCCTGGTTACCAGAATAAAAGAGTCTTTAACATAGTAGTCAGTACCACCCTGGTAATCAGCTGTGTTATAGTAGCTTTGGCTGTACCCTTTTTCGCATATCTCATGGCACTTGTTGGAGCGTTTTTGAGTGTCACAGCTTCAATTATAATGCCATGCTTTTGCTACCTTAAGATTTCTGGCACTTATCGAAAAATAGGATGTGAGATGTTGATCATATGGGGTATAATACTACTAGGTGCTGCAGTAGTCATTTTTGGTACTTATACATCTCTGGTAGAAATAATTGGGCATCTGTAG

Protein Analysis

335

Amino Acids

36.63

Weight (kDa)

8.84

Isoelectric Point (pI)

30.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 5 - 327 2.2e-47 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 857
AccB7I CCANNNNNTGG 1 cut(s) 167
AccII CGCG 1 cut(s) 48
AclWI GGATC 1 cut(s) 328
AcsI RAATTY 1 cut(s) 662
AfaI GTAC 5 cut(s) 458, 554, 754, 795, 970
AfiI CCNNNNNNNGG 3 cut(s) 38, 130, 167
AflII CTTAAG 1 cut(s) 878
AflIII ACRYGT 1 cut(s) 153
AgsI TTSAA 3 cut(s) 176, 578, 852
AjnI CCWGG 3 cut(s) 642, 712, 759
AjuI GAANNNNNNNTTGG 2 cut(s) 36, 68
AluBI AGCT 5 cut(s) 411, 618, 771, 785, 848
AluI AGCT 5 cut(s) 411, 618, 771, 785, 848
Alw21I GWGCWC 2 cut(s) 343, 436
AlwI GGATC 1 cut(s) 328
ApeKI GCWGC 3 cut(s) 224, 695, 950
ApoI RAATTY 1 cut(s) 662
AspLEI GCGC 1 cut(s) 50
AsuHPI GGTGA 1 cut(s) 366
Bbv12I GWGCWC 2 cut(s) 343, 436
BbvI GCAGC 3 cut(s) 211, 682, 937
BccI CCATC 3 cut(s) 530, 667, 699
BciT130I CCWGG 3 cut(s) 644, 714, 761
BclI TGATCA 1 cut(s) 921
BfaI CTAG 4 cut(s) 15, 288, 606, 944
BfmI CTRYAG 2 cut(s) 951, 1004
BfrI CTTAAG 1 cut(s) 878
BisI GCNGC 3 cut(s) 225, 696, 951
BlsI GCNGC 3 cut(s) 226, 697, 952
Bme1390I CCNGG 3 cut(s) 644, 714, 761
BmrFI CCNGG 3 cut(s) 644, 714, 761
BmsI GCATC 1 cut(s) 542
BpuEI CTTGAG 1 cut(s) 283
BsaBI GATNNNNATC 1 cut(s) 920
BsaJI CCNNGG 2 cut(s) 642, 759
Bsc4I CCNNNNNNNGG 3 cut(s) 38, 130, 167
Bse1I ACTGG 1 cut(s) 340
Bse8I GATNNNNATC 1 cut(s) 920
BseBI CCWGG 3 cut(s) 644, 714, 761
BseDI CCNNGG 2 cut(s) 642, 759
BseGI GGATG 2 cut(s) 436, 914
BseJI GATNNNNATC 1 cut(s) 920
BseLI CCNNNNNNNGG 3 cut(s) 38, 130, 167
BseNI ACTGG 1 cut(s) 340
BseXI GCAGC 3 cut(s) 211, 682, 937
Bsh1236I CGCG 1 cut(s) 48
BsiHKAI GWGCWC 2 cut(s) 343, 436
BslI CCNNNNNNNGG 3 cut(s) 38, 130, 167
BsmI GAATGC 2 cut(s) 212, 697
Bsp1286I GDGCHC 2 cut(s) 343, 436
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 2 cut(s) 320, 921
BspFNI CGCG 1 cut(s) 48
BspHI TCATGA 1 cut(s) 87
BspMAI CTGCAG 1 cut(s) 955
BspPI GGATC 1 cut(s) 328
BspTI CTTAAG 1 cut(s) 878
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 1 cut(s) 340
BssECI CCNNGG 2 cut(s) 642, 759
BssMI GATC 2 cut(s) 320, 921
Bst2UI CCWGG 3 cut(s) 644, 714, 761
Bst4CI ACNGT 3 cut(s) 247, 483, 574
BstAFI CTTAAG 1 cut(s) 878
BstAUI TGTACA 1 cut(s) 456
BstDEI CTNAG 2 cut(s) 94, 106
BstEII GGTNACC 2 cut(s) 372, 716
BstENI CCTNNNNNAGG 1 cut(s) 36
BstF5I GGATG 2 cut(s) 436, 914
BstFNI CGCG 1 cut(s) 48
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 2 cut(s) 323, 924
BstMBI GATC 2 cut(s) 320, 921
BstMWI GCNNNNNNNGC 2 cut(s) 539, 615
BstNI CCWGG 3 cut(s) 644, 714, 761
BstNSI RCATGY 1 cut(s) 157
BstPI GGTNACC 2 cut(s) 372, 716
BstSCI CCNGG 3 cut(s) 642, 712, 759
BstSFI CTRYAG 2 cut(s) 951, 1004
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 3 cut(s) 211, 682, 937
BstXI CCANNNNNNTGG 1 cut(s) 249
BtsCI GGATG 2 cut(s) 436, 914
CciI TCATGA 1 cut(s) 87
CfoI GCGC 1 cut(s) 50
Csp6I GTAC 5 cut(s) 457, 553, 753, 794, 969
CviAII CATG 4 cut(s) 88, 154, 814, 864
CviJI RGCY 8 cut(s) 267, 302, 411, 618, 771, 785, 791, 848
CviKI_1 RGCY 8 cut(s) 267, 302, 411, 618, 771, 785, 791, 848
CviQI GTAC 5 cut(s) 457, 553, 753, 794, 969
DdeI CTNAG 2 cut(s) 94, 106
DpnI GATC 2 cut(s) 322, 923
DpnII GATC 2 cut(s) 320, 921
DraI TTTAAA 1 cut(s) 190
Eco91I GGTNACC 2 cut(s) 372, 716
EcoNI CCTNNNNNAGG 1 cut(s) 36
EcoO65I GGTNACC 2 cut(s) 372, 716
EcoRII CCWGG 3 cut(s) 642, 712, 759
EcoT22I ATGCAT 1 cut(s) 535
FaeI CATG 4 cut(s) 91, 157, 817, 867
FatI CATG 4 cut(s) 87, 153, 813, 863
FauNDI CATATG 1 cut(s) 926
FbaI TGATCA 1 cut(s) 921
Fnu4HI GCNGC 3 cut(s) 225, 696, 951
FokI GGATG 2 cut(s) 423, 921
Fsp4HI GCNGC 3 cut(s) 225, 696, 951
FspBI CTAG 4 cut(s) 15, 288, 606, 944
GlaI GCGC 1 cut(s) 49
GluI GCNGC 3 cut(s) 225, 696, 951
HhaI GCGC 1 cut(s) 50
Hin1II CATG 4 cut(s) 91, 157, 817, 867
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HincII GTYRAC 1 cut(s) 570
HindII GTYRAC 1 cut(s) 570
HinfI GANTC 3 cut(s) 11, 393, 732
HphI GGTGA 1 cut(s) 366
Hpy166II GTNNAC 3 cut(s) 333, 459, 570
Hpy188I TCNGA 1 cut(s) 366
Hpy188III TCNNGA 5 cut(s) 32, 88, 124, 344, 601
Hpy8I GTNNAC 3 cut(s) 333, 459, 570
HpyAV CCTTC 4 cut(s) 128, 238, 278, 361
HpyCH4III ACNGT 3 cut(s) 247, 483, 574
HpyCH4V TGCA 4 cut(s) 519, 533, 698, 953
HpyF10VI GCNNNNNNNGC 2 cut(s) 539, 615
HpyF3I CTNAG 2 cut(s) 94, 106
Hsp92II CATG 4 cut(s) 91, 157, 817, 867
HspAI GCGC 1 cut(s) 48
Ksp22I TGATCA 1 cut(s) 921
Kzo9I GATC 2 cut(s) 320, 921
LmnI GCTCC 1 cut(s) 827
Lsp1109I GCAGC 3 cut(s) 211, 682, 937
LweI GCATC 1 cut(s) 542
MaeI CTAG 4 cut(s) 15, 288, 606, 944
MaeIII GTNAC 4 cut(s) 372, 676, 716, 841
MalI GATC 2 cut(s) 322, 923
MboI GATC 2 cut(s) 320, 921
MhlI GDGCHC 2 cut(s) 343, 436
MluCI AATT 7 cut(s) 81, 192, 324, 612, 662, 852, 993
MlyI GAGTC 1 cut(s) 741
MmeI TCCRAC 3 cut(s) 185, 334, 805
MnlI CCTC 2 cut(s) 175, 608
Mph1103I ATGCAT 1 cut(s) 535
MseI TTAA 5 cut(s) 189, 381, 557, 738, 879
MslI CAYNNNNRTG 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 771
MspCI CTTAAG 1 cut(s) 878
MspR9I CCNGG 3 cut(s) 644, 714, 761
Mva1269I GAATGC 2 cut(s) 212, 697
MvaI CCWGG 3 cut(s) 644, 714, 761
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 2 cut(s) 539, 615
NdeI CATATG 1 cut(s) 926
NdeII GATC 2 cut(s) 320, 921
NlaIII CATG 4 cut(s) 91, 157, 817, 867
NmuCI GTSAC 2 cut(s) 372, 841
NsiI ATGCAT 1 cut(s) 535
NspI RCATGY 1 cut(s) 157
PagI TCATGA 1 cut(s) 87
PciI ACATGT 1 cut(s) 153
PctI GAATGC 2 cut(s) 212, 697
PfeI GAWTC 2 cut(s) 11, 393
PflMI CCANNNNNTGG 1 cut(s) 167
PkrI GCNGC 3 cut(s) 226, 697, 952
PleI GAGTC 1 cut(s) 740
PpsI GAGTC 1 cut(s) 740
PscI ACATGT 1 cut(s) 153
PsiI TTATAA 1 cut(s) 857
Psp6I CCWGG 3 cut(s) 642, 712, 759
PspEI GGTNACC 2 cut(s) 372, 716
PspGI CCWGG 3 cut(s) 642, 712, 759
PsrI GAACNNNNNNTAC 2 cut(s) 545, 577
PstI CTGCAG 1 cut(s) 955
PvuII CAGCTG 1 cut(s) 771
RsaI GTAC 5 cut(s) 458, 554, 754, 795, 970
RsaNI GTAC 5 cut(s) 457, 553, 753, 794, 969
RseI CAYNNNNRTG 1 cut(s) 464
SaqAI TTAA 5 cut(s) 189, 381, 557, 738, 879
SatI GCNGC 3 cut(s) 225, 696, 951
Sau3AI GATC 2 cut(s) 320, 921
SchI GAGTC 1 cut(s) 741
ScrFI CCNGG 3 cut(s) 644, 714, 761
SduI GDGCHC 2 cut(s) 343, 436
SetI ASST 9 cut(s) 121, 413, 600, 620, 773, 787, 850, 879, 949
SfaNI GCATC 1 cut(s) 542
SfcI CTRYAG 2 cut(s) 951, 1004
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 2 cut(s) 262, 878
SmoI CTYRAG 2 cut(s) 262, 878
Sse9I AATT 7 cut(s) 81, 192, 324, 612, 662, 852, 993
SspI AATATT 1 cut(s) 72
SspMI CTAG 4 cut(s) 15, 288, 606, 944
StyD4I CCNGG 3 cut(s) 642, 712, 759
TaaI ACNGT 3 cut(s) 247, 483, 574
TaqI TCGA 1 cut(s) 898
TasI AATT 7 cut(s) 81, 192, 324, 612, 662, 852, 993
TatI WGTACW 1 cut(s) 456
TfiI GAWTC 2 cut(s) 11, 393
Tru1I TTAA 5 cut(s) 189, 381, 557, 738, 879
Tru9I TTAA 5 cut(s) 189, 381, 557, 738, 879
TseFI GTSAC 2 cut(s) 372, 841
TseI GCWGC 3 cut(s) 224, 695, 950
Tsp45I GTSAC 2 cut(s) 372, 841
TspDTI ATGAA 7 cut(s) 76, 104, 202, 220, 350, 482, 704
TspGWI ACGGA 1 cut(s) 363
Van91I CCANNNNNTGG 1 cut(s) 167
Vha464I CTTAAG 1 cut(s) 878
XagI CCTNNNNNAGG 1 cut(s) 36
XapI RAATTY 1 cut(s) 662
XceI RCATGY 1 cut(s) 157
XspI CTAG 4 cut(s) 15, 288, 606, 944
Zsp2I ATGCAT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.