pycom17g05100

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004453
Physical Location & Seq
Forward (+)
3533503 .. 3534826
1324 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
ATGGCGGCCCTCGTTCTCAGGGTCGCGCAGGACGGCTCCGGCGACTTCCGGACGGTGCAGGAGGCCATAGACGCCGTCCCACTCTGCAATACCCGTCGCACCGTGATTCGCGTCGCTCCCGGCATCTACAGGCAGCCGGTGTACGTGCCCAAGACGAAGAACCTCATCACGCTCGCGGGTCTGGCTCCGGAGCTCACTGTGCTCACCTGGAACAACACCGCCACCAAAATCGAGCACCACCAGGCTCCTCGGGTGATTGGGACTGGGACTTTCGGGTGTGGGAGTGCTATTGTGGAAGGAGAGGATTTTATTGCAGAGAACATTACTTTTGAGAACTCTGCTCCTGAGGGTTCAGGTCAAGCTGTGGCAATTAGAGTAACATCTGATCGATGTGCCTTCTATAACTGCAGGTTCCTTGGATGGCAGGTATACCCATTACCCTGTACTTGCATTATGGAAAACAGTATCTGA

Protein Analysis

157

Amino Acids

16.96

Weight (kDa)

6.09

Isoelectric Point (pI)

35.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 8 - 142 1.3e-27 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 399, 415
AccI GTMKAC 1 cut(s) 429
AccII CGCG 3 cut(s) 26, 111, 176
AccIII TCCGGA 2 cut(s) 48, 187
AciI CCGC 3 cut(s) 5, 176, 219
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 2 cut(s) 143, 445
AjnI CCWGG 2 cut(s) 206, 240
AluBI AGCT 2 cut(s) 193, 362
AluI AGCT 2 cut(s) 193, 362
Alw21I GWGCWC 3 cut(s) 195, 204, 237
AlwNI CAGNNNCTG 1 cut(s) 468
Ama87I CYCGRG 1 cut(s) 249
Aor13HI TCCGGA 2 cut(s) 48, 187
AoxI GGCC 2 cut(s) 6, 63
ApeKI GCWGC 1 cut(s) 133
AspLEI GCGC 1 cut(s) 28
AspS9I GGNCC 1 cut(s) 7
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 196, 265
AvaI CYCGRG 1 cut(s) 249
AxyI CCTNAGG 1 cut(s) 345
BaeGI GKGCMC 1 cut(s) 150
BanII GRGCYC 1 cut(s) 195
Bbv12I GWGCWC 3 cut(s) 195, 204, 237
BbvI GCAGC 1 cut(s) 145
BccI CCATC 1 cut(s) 414
BceAI ACGGC 2 cut(s) 49, 59
BciT130I CCWGG 2 cut(s) 208, 242
BcnI CCSGG 1 cut(s) 120
BfmI CTRYAG 2 cut(s) 127, 406
BfuAI ACCTGC 2 cut(s) 399, 415
BisI GCNGC 2 cut(s) 6, 134
BlsI GCNGC 2 cut(s) 7, 135
Bme1390I CCNGG 3 cut(s) 120, 208, 242
BmeT110I CYCGRG 1 cut(s) 249
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 4 cut(s) 37, 186, 246, 413
BmrFI CCNGG 3 cut(s) 120, 208, 242
BmrI ACTGGG 1 cut(s) 273
BmsI GCATC 1 cut(s) 132
BmuI ACTGGG 1 cut(s) 273
BpuMI CCSGG 1 cut(s) 120
Bsa29I ATCGAT 1 cut(s) 388
BsaAI YACGTR 1 cut(s) 145
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 2 cut(s) 248, 415
BsaWI WCCGGW 2 cut(s) 48, 187
Bse118I RCCGGY 1 cut(s) 136
Bse1I ACTGG 1 cut(s) 268
Bse21I CCTNAGG 1 cut(s) 345
BseAI TCCGGA 2 cut(s) 48, 187
BseBI CCWGG 2 cut(s) 208, 242
BseCI ATCGAT 1 cut(s) 388
BseDI CCNNGG 2 cut(s) 248, 415
BseGI GGATG 1 cut(s) 425
BseMII CTCAG 2 cut(s) 31, 336
BseNI ACTGG 1 cut(s) 268
BseRI GAGGAG 1 cut(s) 237
BseSI GKGCMC 1 cut(s) 150
BseXI GCAGC 1 cut(s) 145
BsgI GTGCAG 1 cut(s) 77
Bsh1236I CGCG 3 cut(s) 26, 111, 176
BshFI GGCC 2 cut(s) 8, 65
BshVI ATCGAT 1 cut(s) 388
BsiHKAI GWGCWC 3 cut(s) 195, 204, 237
BsiHKCI CYCGRG 1 cut(s) 249
BsiSI CCGG 5 cut(s) 39, 49, 120, 137, 188
BslFI GGGAC 3 cut(s) 62, 274, 280
BsmFI GGGAC 3 cut(s) 62, 274, 280
BsnI GGCC 2 cut(s) 8, 65
BsoBI CYCGRG 1 cut(s) 249
Bsp1286I GDGCHC 4 cut(s) 150, 195, 204, 237
Bsp13I TCCGGA 2 cut(s) 48, 187
Bsp143I GATC 1 cut(s) 385
BspACI CCGC 3 cut(s) 5, 176, 219
BspANI GGCC 2 cut(s) 8, 65
BspCNI CTCAG 2 cut(s) 30, 337
BspDI ATCGAT 1 cut(s) 388
BspEI TCCGGA 2 cut(s) 48, 187
BspFNI CGCG 3 cut(s) 26, 111, 176
BspLI GGNNCC 4 cut(s) 37, 186, 246, 413
BspMAI CTGCAG 1 cut(s) 410
BspMI ACCTGC 2 cut(s) 399, 415
BsrFI RCCGGY 1 cut(s) 136
BsrI ACTGG 1 cut(s) 268
BssAI RCCGGY 1 cut(s) 136
BssECI CCNNGG 2 cut(s) 248, 415
BssMI GATC 1 cut(s) 385
BssNAI GTATAC 1 cut(s) 430
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 1 cut(s) 415
Bst1107I GTATAC 1 cut(s) 430
Bst2UI CCWGG 2 cut(s) 208, 242
Bst4CI ACNGT 4 cut(s) 55, 103, 199, 464
BstACI GRCGYC 1 cut(s) 72
BstBAI YACGTR 1 cut(s) 145
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 2 cut(s) 17, 345
BstF5I GGATG 1 cut(s) 425
BstFNI CGCG 3 cut(s) 26, 111, 176
BstHHI GCGC 1 cut(s) 28
BstKTI GATC 1 cut(s) 388
BstMBI GATC 1 cut(s) 385
BstMWI GCNNNNNNNGC 3 cut(s) 71, 182, 199
BstNI CCWGG 2 cut(s) 208, 242
BstSCI CCNGG 3 cut(s) 118, 206, 240
BstSFI CTRYAG 2 cut(s) 127, 406
BstSLI GKGCMC 1 cut(s) 150
BstUI CGCG 3 cut(s) 26, 111, 176
BstV1I GCAGC 1 cut(s) 145
BstZ17I GTATAC 1 cut(s) 430
Bsu15I ATCGAT 1 cut(s) 388
Bsu36I CCTNAGG 1 cut(s) 345
BsuRI GGCC 2 cut(s) 8, 65
BsuTUI ATCGAT 1 cut(s) 388
BtsCI GGATG 1 cut(s) 425
BtsIMutI CAGTG 1 cut(s) 195
BveI ACCTGC 2 cut(s) 399, 415
Cac8I GCNNGC 1 cut(s) 174
CaiI CAGNNNCTG 1 cut(s) 468
CfoI GCGC 1 cut(s) 28
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 1 cut(s) 7
ClaI ATCGAT 1 cut(s) 388
CseI GACGC 2 cut(s) 80, 100
Csp6I GTAC 2 cut(s) 142, 444
CviJI RGCY 8 cut(s) 8, 36, 65, 136, 185, 193, 245, 362
CviKI_1 RGCY 8 cut(s) 8, 36, 65, 136, 185, 193, 245, 362
CviQI GTAC 2 cut(s) 142, 444
DdeI CTNAG 2 cut(s) 17, 345
DpnI GATC 1 cut(s) 387
DpnII GATC 1 cut(s) 385
Ecl136II GAGCTC 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 415
Eco24I GRGCYC 1 cut(s) 195
Eco53kI GAGCTC 1 cut(s) 193
Eco81I CCTNAGG 1 cut(s) 345
Eco88I CYCGRG 1 cut(s) 249
EcoICRI GAGCTC 1 cut(s) 193
EcoRII CCWGG 2 cut(s) 206, 240
EcoT14I CCWWGG 1 cut(s) 415
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 415
FaiI YATR 4 cut(s) 68, 402, 430, 455
FaqI GGGAC 3 cut(s) 62, 274, 280
FauI CCCGC 1 cut(s) 169
FblI GTMKAC 1 cut(s) 429
Fnu4HI GCNGC 2 cut(s) 6, 134
FokI GGATG 1 cut(s) 432
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 2 cut(s) 6, 134
GlaI GCGC 1 cut(s) 27
GluI GCNGC 2 cut(s) 6, 134
HaeIII GGCC 2 cut(s) 8, 65
HapII CCGG 5 cut(s) 39, 49, 120, 137, 188
HgaI GACGC 2 cut(s) 80, 100
HhaI GCGC 1 cut(s) 28
Hin1I GRCGYC 1 cut(s) 72
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HinfI GANTC 1 cut(s) 106
HpaII CCGG 5 cut(s) 39, 49, 120, 137, 188
HphI GGTGA 2 cut(s) 196, 265
Hpy166II GTNNAC 2 cut(s) 142, 430
Hpy188I TCNGA 2 cut(s) 385, 470
Hpy188III TCNNGA 3 cut(s) 49, 188, 344
Hpy8I GTNNAC 2 cut(s) 142, 430
Hpy99I CGWCG 2 cut(s) 99, 116
HpyAV CCTTC 2 cut(s) 290, 406
HpyCH4III ACNGT 4 cut(s) 55, 103, 199, 464
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 5 cut(s) 58, 87, 314, 408, 450
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 182, 199
HpyF3I CTNAG 2 cut(s) 17, 345
HpySE526I ACGT 1 cut(s) 144
Hsp92I GRCGYC 1 cut(s) 72
HspAI GCGC 1 cut(s) 26
Kpn2I TCCGGA 2 cut(s) 48, 187
Kzo9I GATC 1 cut(s) 385
LmnI GCTCC 6 cut(s) 41, 121, 190, 190, 250, 346
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 1 cut(s) 132
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 1 cut(s) 376
MalI GATC 1 cut(s) 387
MboI GATC 1 cut(s) 385
MboII GAAGA 1 cut(s) 169
MhlI GDGCHC 4 cut(s) 150, 195, 204, 237
MluCI AATT 1 cut(s) 369
MnlI CCTC 6 cut(s) 20, 55, 173, 258, 295, 340
MroI TCCGGA 2 cut(s) 48, 187
MspI CCGG 5 cut(s) 39, 49, 120, 137, 188
MspR9I CCNGG 3 cut(s) 120, 208, 242
MvaI CCWGG 2 cut(s) 208, 242
MvnI CGCG 3 cut(s) 26, 111, 176
MwoI GCNNNNNNNGC 3 cut(s) 71, 182, 199
NciI CCSGG 1 cut(s) 120
NdeII GATC 1 cut(s) 385
NlaIV GGNNCC 4 cut(s) 37, 186, 246, 413
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PfeI GAWTC 1 cut(s) 106
PflFI GACNNNGTC 1 cut(s) 74
PkrI GCNGC 2 cut(s) 7, 135
Ppu21I YACGTR 1 cut(s) 145
Psp124BI GAGCTC 1 cut(s) 195
Psp6I CCWGG 2 cut(s) 206, 240
PspGI CCWGG 2 cut(s) 206, 240
PspN4I GGNNCC 4 cut(s) 37, 186, 246, 413
PspPI GGNCC 1 cut(s) 7
PstI CTGCAG 1 cut(s) 410
PstNI CAGNNNCTG 1 cut(s) 468
PsyI GACNNNGTC 1 cut(s) 74
RsaI GTAC 2 cut(s) 143, 445
RsaNI GTAC 2 cut(s) 142, 444
SacI GAGCTC 1 cut(s) 195
SatI GCNGC 2 cut(s) 6, 134
Sau3AI GATC 1 cut(s) 385
Sau96I GGNCC 1 cut(s) 7
ScrFI CCNGG 3 cut(s) 120, 208, 242
SduI GDGCHC 4 cut(s) 150, 195, 204, 237
SetI ASST 8 cut(s) 147, 165, 195, 209, 358, 364, 413, 429
SfaNI GCATC 1 cut(s) 132
SfcI CTRYAG 2 cut(s) 127, 406
Sse9I AATT 1 cut(s) 369
SsiI CCGC 3 cut(s) 5, 176, 219
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 3 cut(s) 118, 206, 240
StyI CCWWGG 1 cut(s) 415
TaaI ACNGT 4 cut(s) 55, 103, 199, 464
TaiI ACGT 1 cut(s) 147
TaqI TCGA 2 cut(s) 231, 388
TasI AATT 1 cut(s) 369
TatI WGTACW 1 cut(s) 443
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 106
TscAI CASTG 1 cut(s) 202
TseI GCWGC 1 cut(s) 133
TspRI CASTG 1 cut(s) 202
Tth111I GACNNNGTC 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.