pycom17g10990

Rrp15p

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0009316
Physical Location & Seq
Forward (+)
8522022 .. 8524665
2644 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 960 bp
ATGGCCGAAGAAACCCATCTGCCAGAAGCAGCAACAGAGCCAAGGAAGCGGAAAATAGGGAAGAAGAAAGGCTCCAAGGCAAAGAAGAAGGCGAAGATGTTACATTCGTCAGAGGAAATCAGAGCCATGAAGCCGAAGAAGTTTAACAATAAAATGAAGAAACTGTTTCTGAAACGAGCTAAGGACTACAATTCGGAGTCGGAGGAGGAGTCCAAACAGGAAGATGTGAAACCGAAACCGTTGGGAAACAATGAGGAGGAGCCGAGTGGCGGTGCCGCGTCGTCTTCAGGAGAAGAGTCGGAGGAAGGTGGTGGTGGTGGTGGTGGTCGCAATAGGGGTGATGGAGTTTCAGATGACGAGGAGCACGGCGAGATTCTGCCCGGAATCATGAAGTTTTCGGAGGGTTGCAATGCGTTTAGGTTGGCATTCAAGAGTATAATCAAGAAGTCAGTTTCTCTGAATGCAGTGGGACCAGTGATGTCGGCGCACAAGAAGCTTGTTGCAGAAAAGCTTGCCGAGGAGCAGACGGAGCGCAAGGCCAAGGGCGAGGCTAAGAAGGAAAAGCATACGGTGATAGAAAAGGGGCATGTGATGCCGGCCAATTATTTGGACACGCATGAAAAGTTTCTAATCGGTGTTGCTACTAAAGGGGTGGTGAAGTTGTTCAATGCTGTCAACAAGGCACAAAATGCTCAGAAAGGACTGAATCCATCCAAGTTTAAAGATGCCAAAGTGATAAAAAAGCGGAGGAAAGAAGCATTCTTTTCAGAGTTGGGCAAAACGTCTTCGAAGGCTCATGCGTCTAAAGGCGCAGTGGATGGTGAAGGGCCAGCCTGGGCTCCCTTAAGAGATAATTACATGCTCACAAATCCCAAATTGAAAGACTGGGATAAGATGCCGGACACCGTTGTGGACGATGACATTGGAAGGGTCGCTGGAGATAGTTCCGATGACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rrp15p

Protein Analysis

320

Amino Acids

34.87

Weight (kDa)

9.32

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rrp15p PF07890 139 - 258 1.3e-20 Rrp15p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 272
AccII CGCG 1 cut(s) 278
AciI CCGC 4 cut(s) 49, 270, 276, 745
AcoI YGGCCR 2 cut(s) 3, 597
AcuI CTGAAG 1 cut(s) 270
AfiI CCNNNNNNNGG 1 cut(s) 269
AflII CTTAAG 1 cut(s) 844
AgsI TTSAA 3 cut(s) 430, 667, 880
AjnI CCWGG 1 cut(s) 833
AleI CACNNNNGTG 1 cut(s) 908
AluBI AGCT 3 cut(s) 179, 496, 511
AluI AGCT 3 cut(s) 179, 496, 511
Alw21I GWGCWC 1 cut(s) 366
AoxI GGCC 4 cut(s) 3, 537, 597, 827
ApeKI GCWGC 1 cut(s) 29
Asp700I GAANNNNTTC 2 cut(s) 624, 662
AspLEI GCGC 3 cut(s) 487, 534, 812
AspS9I GGNCC 2 cut(s) 470, 827
AsuC2I CCSGG 1 cut(s) 381
AsuHPI GGTGA 4 cut(s) 350, 583, 667, 833
AsuII TTCGAA 1 cut(s) 788
AvaII GGWCC 1 cut(s) 470
BanI GGYRCC 1 cut(s) 272
BanII GRGCYC 1 cut(s) 841
BbsI GAAGAC 2 cut(s) 276, 777
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 1 cut(s) 41
BccI CCATC 4 cut(s) 24, 335, 718, 812
BceAI ACGGC 1 cut(s) 382
BciT130I CCWGG 1 cut(s) 835
BcnI CCSGG 1 cut(s) 381
BfrI CTTAAG 1 cut(s) 844
BisI GCNGC 2 cut(s) 30, 276
BlsI GCNGC 2 cut(s) 31, 277
Bme1390I CCNGG 2 cut(s) 381, 835
Bme18I GGWCC 1 cut(s) 470
BmgT120I GGNCC 2 cut(s) 470, 827
BmiI GGNNCC 5 cut(s) 73, 261, 274, 471, 840
BmrFI CCNGG 2 cut(s) 381, 835
BmrI ACTGGG 1 cut(s) 895
BmsI GCATC 3 cut(s) 582, 715, 885
BmuI ACTGGG 1 cut(s) 895
BpiI GAAGAC 2 cut(s) 276, 777
BpmI CTGGAG 1 cut(s) 957
Bpu10I CCTNAGC 1 cut(s) 180
Bpu14I TTCGAA 1 cut(s) 788
BpuMI CCSGG 1 cut(s) 381
BsaJI CCNNGG 5 cut(s) 41, 75, 516, 540, 834
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse118I RCCGGY 1 cut(s) 595
Bse1I ACTGG 2 cut(s) 473, 890
Bse3DI GCAATG 1 cut(s) 415
BseBI CCWGG 1 cut(s) 835
BseDI CCNNGG 5 cut(s) 41, 75, 516, 540, 834
BseGI GGATG 2 cut(s) 710, 823
BseLI CCNNNNNNNGG 1 cut(s) 269
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 1 cut(s) 707
BseNI ACTGG 2 cut(s) 473, 890
BseRI GAGGAG 6 cut(s) 218, 221, 269, 272, 374, 533
BseXI GCAGC 1 cut(s) 41
Bsh1236I CGCG 1 cut(s) 278
BshFI GGCC 4 cut(s) 5, 539, 599, 829
BshNI GGYRCC 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 366
BsiSI CCGG 3 cut(s) 381, 596, 899
BslFI GGGAC 1 cut(s) 483
BslI CCNNNNNNNGG 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 483
BsmI GAATGC 3 cut(s) 425, 466, 758
BsnI GGCC 4 cut(s) 5, 539, 599, 829
Bsp119I TTCGAA 1 cut(s) 788
Bsp1286I GDGCHC 2 cut(s) 366, 841
BspACI CCGC 4 cut(s) 49, 270, 276, 745
BspANI GGCC 4 cut(s) 5, 539, 599, 829
BspCNI CTCAG 1 cut(s) 706
BspFNI CGCG 1 cut(s) 278
BspHI TCATGA 1 cut(s) 387
BspLI GGNNCC 5 cut(s) 73, 261, 274, 471, 840
BspT104I TTCGAA 1 cut(s) 788
BspT107I GGYRCC 1 cut(s) 272
BspTI CTTAAG 1 cut(s) 844
BsrDI GCAATG 1 cut(s) 415
BsrFI RCCGGY 1 cut(s) 595
BsrI ACTGG 2 cut(s) 473, 890
BssAI RCCGGY 1 cut(s) 595
BssECI CCNNGG 5 cut(s) 41, 75, 516, 540, 834
BssT1I CCWWGG 3 cut(s) 41, 75, 540
Bst2UI CCWGG 1 cut(s) 835
Bst4CI ACNGT 4 cut(s) 165, 240, 571, 907
Bst6I CTCTTC 1 cut(s) 288
BstAFI CTTAAG 1 cut(s) 844
BstAPI GCANNNNNTGC 2 cut(s) 592, 689
BstBI TTCGAA 1 cut(s) 788
BstC8I GCNNGC 3 cut(s) 513, 597, 831
BstDEI CTNAG 3 cut(s) 180, 552, 693
BstF5I GGATG 2 cut(s) 710, 823
BstFNI CGCG 1 cut(s) 278
BstHHI GCGC 3 cut(s) 487, 534, 812
BstMWI GCNNNNNNNGC 5 cut(s) 46, 493, 529, 592, 689
BstNI CCWGG 1 cut(s) 835
BstNSI RCATGY 2 cut(s) 590, 862
BstSCI CCNGG 2 cut(s) 379, 833
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 1 cut(s) 41
BstV2I GAAGAC 2 cut(s) 276, 777
BstXI CCANNNNNNTGG 1 cut(s) 607
BsuRI GGCC 4 cut(s) 5, 539, 599, 829
BtsCI GGATG 2 cut(s) 710, 823
BtsI GCAGTG 2 cut(s) 471, 819
BtsIMutI CAGTG 3 cut(s) 471, 480, 819
Cac8I GCNNGC 3 cut(s) 513, 597, 831
CciI TCATGA 1 cut(s) 387
CfoI GCGC 3 cut(s) 487, 534, 812
Cfr10I RCCGGY 1 cut(s) 595
Cfr13I GGNCC 2 cut(s) 470, 827
CseI GACGC 2 cut(s) 267, 789
CviAII CATG 6 cut(s) 127, 388, 587, 617, 797, 859
DdeI CTNAG 3 cut(s) 180, 552, 693
DraI TTTAAA 1 cut(s) 721
EaeI YGGCCR 2 cut(s) 3, 597
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Eco130I CCWWGG 3 cut(s) 41, 75, 540
Eco24I GRGCYC 1 cut(s) 841
Eco47I GGWCC 1 cut(s) 470
Eco57I CTGAAG 1 cut(s) 270
EcoRII CCWGG 1 cut(s) 833
EcoT14I CCWWGG 3 cut(s) 41, 75, 540
EcoT38I GRGCYC 1 cut(s) 841
ErhI CCWWGG 3 cut(s) 41, 75, 540
FaeI CATG 6 cut(s) 130, 391, 590, 620, 800, 862
FaiI YATR 8 cut(s) 128, 389, 437, 567, 588, 618, 798, 860
FaqI GGGAC 1 cut(s) 483
FatI CATG 6 cut(s) 126, 387, 586, 616, 796, 858
Fnu4HI GCNGC 2 cut(s) 30, 276
FokI GGATG 2 cut(s) 697, 830
FriOI GRGCYC 1 cut(s) 841
Fsp4HI GCNGC 2 cut(s) 30, 276
GlaI GCGC 3 cut(s) 486, 533, 811
GluI GCNGC 2 cut(s) 30, 276
GsuI CTGGAG 1 cut(s) 957
HaeIII GGCC 4 cut(s) 5, 539, 599, 829
HapII CCGG 3 cut(s) 381, 596, 899
HgaI GACGC 2 cut(s) 267, 789
HhaI GCGC 3 cut(s) 487, 534, 812
Hin1II CATG 6 cut(s) 130, 391, 590, 620, 800, 862
Hin6I GCGC 3 cut(s) 485, 532, 810
HinP1I GCGC 3 cut(s) 485, 532, 810
HincII GTYRAC 1 cut(s) 676
HindII GTYRAC 1 cut(s) 676
HindIII AAGCTT 2 cut(s) 494, 509
HinfI GANTC 6 cut(s) 197, 209, 296, 373, 384, 706
HpaII CCGG 3 cut(s) 381, 596, 899
HphI GGTGA 4 cut(s) 350, 583, 667, 833
Hpy166II GTNNAC 2 cut(s) 676, 913
Hpy188III TCNNGA 4 cut(s) 288, 388, 430, 442
Hpy8I GTNNAC 2 cut(s) 676, 913
Hpy99I CGWCG 1 cut(s) 283
HpyAV CCTTC 6 cut(s) 82, 299, 550, 784, 818, 921
HpyCH4III ACNGT 4 cut(s) 165, 240, 571, 907
HpyCH4IV ACGT 1 cut(s) 782
HpyCH4V TGCA 3 cut(s) 408, 464, 503
HpyF10VI GCNNNNNNNGC 5 cut(s) 46, 493, 529, 592, 689
HpyF3I CTNAG 3 cut(s) 180, 552, 693
HpySE526I ACGT 1 cut(s) 782
Hsp92II CATG 6 cut(s) 130, 391, 590, 620, 800, 862
HspAI GCGC 3 cut(s) 485, 532, 810
KroI GCCGGC 1 cut(s) 595
KroNI GCCGGC 1 cut(s) 597
LmnI GCTCC 6 cut(s) 77, 259, 361, 520, 529, 844
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 3 cut(s) 582, 715, 885
MaeII ACGT 1 cut(s) 782
MaeIII GTNAC 1 cut(s) 99
MhlI GDGCHC 2 cut(s) 366, 841
MluCI AATT 4 cut(s) 190, 601, 853, 875
MlyI GAGTC 3 cut(s) 206, 218, 305
MmeI TCCRAC 2 cut(s) 180, 279
MroNI GCCGGC 1 cut(s) 595
MroXI GAANNNNTTC 2 cut(s) 624, 662
MseI TTAA 3 cut(s) 144, 720, 845
MslI CAYNNNNRTG 1 cut(s) 908
MspCI CTTAAG 1 cut(s) 844
MspI CCGG 3 cut(s) 381, 596, 899
MspR9I CCNGG 2 cut(s) 381, 835
Mva1269I GAATGC 3 cut(s) 425, 466, 758
MvaI CCWGG 1 cut(s) 835
MvnI CGCG 1 cut(s) 278
MwoI GCNNNNNNNGC 5 cut(s) 46, 493, 529, 592, 689
NaeI GCCGGC 1 cut(s) 597
NciI CCSGG 1 cut(s) 381
NgoMIV GCCGGC 1 cut(s) 595
NlaIII CATG 6 cut(s) 130, 391, 590, 620, 800, 862
NlaIV GGNNCC 5 cut(s) 73, 261, 274, 471, 840
NmeAIII GCCGAG 2 cut(s) 288, 541
NspI RCATGY 2 cut(s) 590, 862
NspV TTCGAA 1 cut(s) 788
OliI CACNNNNGTG 1 cut(s) 908
PagI TCATGA 1 cut(s) 387
PctI GAATGC 3 cut(s) 425, 466, 758
PdiI GCCGGC 1 cut(s) 597
PdmI GAANNNNTTC 2 cut(s) 624, 662
PfeI GAWTC 3 cut(s) 373, 384, 706
PkrI GCNGC 2 cut(s) 31, 277
PleI GAGTC 3 cut(s) 205, 217, 304
PpsI GAGTC 3 cut(s) 205, 217, 304
Psp6I CCWGG 1 cut(s) 833
PspGI CCWGG 1 cut(s) 833
PspN4I GGNNCC 5 cut(s) 73, 261, 274, 471, 840
PspPI GGNCC 2 cut(s) 470, 827
RseI CAYNNNNRTG 1 cut(s) 908
SaqAI TTAA 3 cut(s) 144, 720, 845
SatI GCNGC 2 cut(s) 30, 276
Sau96I GGNCC 2 cut(s) 470, 827
SchI GAGTC 3 cut(s) 206, 218, 305
ScrFI CCNGG 2 cut(s) 381, 835
SduI GDGCHC 2 cut(s) 366, 841
SetI ASST 6 cut(s) 181, 310, 422, 498, 513, 785
SfaNI GCATC 3 cut(s) 582, 715, 885
SfuI TTCGAA 1 cut(s) 788
SinI GGWCC 1 cut(s) 470
SmiMI CAYNNNNRTG 1 cut(s) 908
SmlI CTYRAG 1 cut(s) 844
SmoI CTYRAG 1 cut(s) 844
Sse9I AATT 4 cut(s) 190, 601, 853, 875
SsiI CCGC 4 cut(s) 49, 270, 276, 745
StyD4I CCNGG 2 cut(s) 379, 833
StyI CCWWGG 3 cut(s) 41, 75, 540
TaaI ACNGT 4 cut(s) 165, 240, 571, 907
TaiI ACGT 1 cut(s) 785
TaqI TCGA 1 cut(s) 788
TasI AATT 4 cut(s) 190, 601, 853, 875
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 3 cut(s) 373, 384, 706
Tru1I TTAA 3 cut(s) 144, 720, 845
Tru9I TTAA 3 cut(s) 144, 720, 845
TscAI CASTG 3 cut(s) 471, 480, 819
TseI GCWGC 1 cut(s) 29
TspDTI ATGAA 4 cut(s) 143, 170, 404, 633
TspGWI ACGGA 1 cut(s) 542
TspRI CASTG 3 cut(s) 471, 480, 819
Vha464I CTTAAG 1 cut(s) 844
VpaK11BI GGWCC 1 cut(s) 470
XceI RCATGY 2 cut(s) 590, 862
XmnI GAANNNNTTC 2 cut(s) 624, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.