pycom17g13080

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0009173
Physical Location & Seq
Forward (+)
10761271 .. 10775242
13972 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4248 bp
ATGGCGAGCTCCAGCGGGACGAAGACCGACTACCCGGGGGGGCTTCCGCGGTCGTTTTCGAGGGGAATGACGAGAATGTCCACTAAGGTTTTGGACATGCCGCCCGAGGACGAGGCGTCGGACATTAATGTGCCCCCGGGACTTCCCTCAATTGCTCCGATTTTTCGAGTCGCCAATGAGATTGAGGAGGACAACCCTAGGGTTGCTTATCTCTGCCGCTTCCATGGATTTGAGAAGGCACACAATTTGGATCCAACATCAAGTGGACGTGGTGTTCGACAGTTTAAGACGCTTCTTTTGCACAGACTTGAAAAGGAAGAAACAGAGACAAATCATCAGCTTAGTAGATCTGATCCACAAGAAATTCTGAAGTATTACCACCAATTCTATGAGAAAAATATCAAAGATGGAGAGTACACAAAGACACCGGAAGAGATGGCTAAGAATTGTCAGATTGCAACAGTACTGTATGATGTGCTCCAGACAGTGGTACCTCAAACCCAAATAGATAAACAGACAAAGAAGATGGCTGAAGATGTCAAGAAGAAAAGGGAGCAGTATGTCAACTATAACATTCTTCCGTTATATACTGTTGGTGTTAAACCAGCAATTATGGAACTTCCCGAGATCAAAGCAGCGCTTCGTGCTGTACAAAATGTGAATGGTCTTCCAATGCCAAGATTTCATGTACAACCCACGAACCCTGAAGACAAGTCAACAATGCCTGCAGAGAGGATTAAACCTGTCAATGATATACTTGATTGGCTTTCCTCTATCTTTGGGTTTCAGAAAGGAAATGTGGCAAATCAGAGGGAACACTTACTCTTGCTACTTGCCAACATGGATGTAAGACATAGAAATCTTGAAAATTACACTGAGTTGAATAGTTCCACGGTACAACGTTTGATGGACAAAGTTTTTAAAAATTATCGTTCCTGGTTTAACTATTTGCATTGTAAATCAAATCTTAGGTTCCCTCGAGATTCTGACAGACGACAATTGGAACTTATTTACATTGCGCTCTATCTTCTCATATGGGGTGAAGCTTCAAATATTCGATTCATGCCTGAATGCTTATGCTATATTTTCCACCAGATGGCCAATGAGATGTATGGAATTTTATTTAGCAATGTTCATCCGGCTACCGGAGAAACATACCAAGCAGCAGCACGGGATGAAGAGTCTTTTTTGAGGGATGTTGTAACTCCGATCTACCAAGTCTTGTACATGGAAGCAAAGAGAAACAAACATGGCACGGCAAGTCATTCAAGATGGAGAAATTATGATGACCTAAATGAATACTTCTGGTCCGACAGATGTTTTAGGCTAGGGTGGCCAATGGATCCTAAAGCAGATTTTTTTAGGCAAGATGAGAGGCCAAATCAAGCTGCTGGTGGGATGAGGAAACCTAAAACAAATTTTGTAGAAGTCCGAACATTTTGGCACCTCTACAGAAGTTTCGATCGAATGTGGATATTCTTGATATTGGCTTTCCAGGCTATGCTTATTGTTGCATGGAGTCCTTCTGGTTCTCTGACTGCTTTCTTTGATGCGGATGTCTTCAGAAGCGTTTTAAGCATCTTCATCACTTATGCTTTCCTCAACTTGTTACAAGCCACTCTGGATATAATTCTTAGTTGGCATTGTTGGAAGAGCTTGAAATTCACTCAAATACTACGATACCTCTTGAAATTCGCTGTAGCTGGTGTATGGGCTGTGGTTCTGCCTATCGGTTATTCTAGTTCTGTGCAGAATCCTACAGGACTTTTGAAATTCTTCAGCAGTTGGGCTAGGGATTGGCGGAATCAATCATTTTATAATTATTCTGTTGCACTCTATCTGCTTCCTAATATATTGGCCGCCGTGCTATTTTTTCTTCCACCTTTACGGAGGCACATAGAGCGCTCAAATTGGAGGATTGTCACTCTTTTTATGTGGTGGGCTCAGCCAAAACTGTATATAGGAAGAGGGCTTCATGAGGACATCTTTTCACTGCTGAAATATACACTTTTTTGGATCATGCTTCTAATCAGCAAGCTATCGTTCAGCTATTTTGTGGAGATACTGCCACTTGTTGCACCAACAAAAATAATAATGGAGATGCCTATAAGTAATTATCAATGGCATGAGTTCTTTCCACATGTAACTCATAATATTGGCGTTGTCATTGCAATATGGGCTCCAATTGTCCTGGTTTATTTTATGGATGCACAAATATGGTATGCAATATTTTCTACTGTCTTTGGTGGGATTCATGGAGCATTCAGCCATTTGGGTGAGATACGGACCCTTGGGATGTTGAGGTCCAGATTTGACTCTGTGCCTTCTGCTTTCTGTAATCGTCTTATGCCGTCAGGCAATAAGGATGCAAAGGAGAAAAGACAATTGGTGGATGAGGCACTCGAAATGTCGCAGGCTGAGGCACTCGAACGAAAGAACATTGATAATTTCGCTAATGTCTGGAATGAGTTCATAAATTCAATGCGATCGGAAGACCTTATCAGCAATAAAGATAAAGATCTGCTGCTTGTTCCATATTCATCAGAAGATGTGACTGTTGTCCAGTGGCCTCCTTTCTTGCTTGCTAGTAAGATTCCAATAGCATTGGACATGGCAAAAGATTTTACAGGGAAGGCAGATGATGATTTATTTAGAAAGATCAAGAGTGATGATTATATGTACTCAGCAGTAATTGAATGCTATGAGACACTCAGGGATATTATATCTGGCCTTCTGGAAGAGGAAGAGGATAAGATGATTGTAAGGCACATATGCAACGAAGTAGACAGTAGTATAGAGCAGCGGAAATTTTTGACTACTTTCCGCATGAGTGGGCTGCCATTTCTTAGCGAGAGGTTGGAGAAATTTCTAAAGCTCTTGCAAGCTGAAGATGAGAATTTCGATAATTCCATGCGTCAGATAATCAATGTCCTTCAAGATATAGTGGAGATTATCACGCAGGATGTTATGATCAACAGCGATGCGATTGCAATACTGGAGAAAGCACATTACAATGATGGTGAAAACGTCAAGGAGCAAAGGTTTCAAAAGCTTAAGATTGACCTTAGACGTAATACATCTCAGAGGGAGAAGGCTTGGATAGACAAGGTAGTGAGACTTCATTTGCTTTTGACCGTCAAAGAGTCTGCCATCAATGTGCCTCAAAACTTGGAAGCTCGCAGGCGCATCACCTTTTTTGCGAACTCCTTATTCATGAACATGCCACGTGCTCCCAAAGTTCGCGACATGCTCTCATTTAGTGTTCTGACTCCTTATTACAAAGAAGATGTCCTCTATTCTGATGAGGAACTTACGAAAGAAAACGAGGATGGAATCTCAATTTTGTTCTACCTGCAGAAAATATATCCTGATGAATGGACAAATTTTGGGGAACGGATAAATGATCCTAAGAACAAGTTTTCTGAGAACAACAAGTCAGAGTTAACCCGTCAGTGGGTATCTTACAGAGCACAGACACTTTCTAGAACAGTGAGAGGGATGATGTACTATAGGCAGGCTCTAGAACTTCAGTGCGTCCTGGAAACAGAAGGAGATAGTGCTTCTTTTGGAGGCTACCAAACCTCAACCTTAAGCGAAAATGACGAGCAGGCTGACCAGGCTCGTGCAAAAGCTCTGGCAGATTTGAAGTTTACTTATGTAGTTTCTTGTCAGGTCTATGGTGCTCAGAAAATCTCCAATGAATCTCGGGAAAAAAGTTATTACACTAATATCCTGAAGCTGATGCTAACGTATTCATCTCTACGTATTGCTTACATAGATACAACGGAGGAGCAAGTGAATGGAAAACCCATAAAAACTTACTATTCTGTTCTAGTCAAGGGTGGTGACAAATGGGATGAGGAAATATACCGCATCAAGCTCCCAGGTCCTCCTACAGATATTGGCGAAGGAAAACCTGAAAATCAAAATCATGCCATTATCTTTACTCGTGGCGAAGCCCTGCAGACAATAGACATGAATCAGGATAATTACTTTGAAGAAGCTTTCAAGATGAGAAATGTATTGGAGGAATTCTTAAAGCATCGTCGTGGGCAACGAAAACCTACAATATTGGGTCTAAGGGAGCATATTTTCACTGGAAGTTTGTTTTGCACTTTATTCCTGGGTTTGGGCGTGGTGAGAGGATTAATATCCTTCAATTCTAGCCACCAGAACTGTAATCCAATCCTCAATTGGTACGGTTCCATTATGGTCATCCCGATATATTTGACAGAATCTTCCACATAA

Protein Analysis

1416

Amino Acids

164.57

Weight (kDa)

6.69

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 50 - 189 1.2e-11 Vta1 like
FKS1_dom1 PF14288 335 - 446 5.1e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 857 - 1046 5.7e-66 Callose synthase 1 domain
Glucan_synthase PF02364 1053 - 1142 2.7e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1142 - 1368 3.2e-62 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1818
AasI GACNNNNNNGTC 1 cut(s) 76
Acc36I ACCTGC 1 cut(s) 3360
Acc65I GGTACC 1 cut(s) 490
AccB1I GGYRCC 2 cut(s) 490, 1443
AccB7I CCANNNNNTGG 2 cut(s) 487, 1096
AccI GTMKAC 1 cut(s) 2784
AccII CGCG 2 cut(s) 49, 3243
AclI AACGTT 1 cut(s) 901
AclWI GGATC 7 cut(s) 245, 258, 347, 1337, 1350, 2024, 3398
AcoI YGGCCR 3 cut(s) 1098, 1334, 1857
AcuI CTGAAG 8 cut(s) 389, 552, 726, 1546, 1762, 2907, 3512, 3755
AcvI CACGTG 1 cut(s) 3227
AcyI GRCGYC 1 cut(s) 116
AfeI AGCGCT 2 cut(s) 639, 1904
AfiI CCNNNNNNNGG 6 cut(s) 487, 1096, 1145, 3453, 3454, 4129
AflII CTTAAG 2 cut(s) 3053, 3589
AflIII ACRYGT 1 cut(s) 2140
AhdI GACNNNNNGTC 1 cut(s) 115
AjiI CACGTC 1 cut(s) 269
AjnI CCWGG 7 cut(s) 935, 1494, 2190, 3537, 3615, 3883, 4122
AjuI GAANNNNNNNTTGG 2 cut(s) 2369, 2401
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
Alw21I GWGCWC 5 cut(s) 11, 480, 3232, 3472, 3685
Alw26I GTCTC 3 cut(s) 320, 2699, 3109
AlwI GGATC 7 cut(s) 245, 258, 347, 1337, 1350, 2024, 3398
Ama87I CYCGRG 6 cut(s) 34, 104, 136, 623, 978, 3705
Aor51HI AGCGCT 2 cut(s) 639, 1904
AoxI GGCC 6 cut(s) 1098, 1334, 1376, 1857, 2567, 2728
ApeKI GCWGC 7 cut(s) 635, 1163, 1166, 1388, 2524, 2800, 2836
ArsI GACNNNNNNTTYG 6 cut(s) 258, 280, 290, 312, 3273, 3305
AseI ATTAAT 2 cut(s) 126, 4148
Asp700I GAANNNNTTC 2 cut(s) 1775, 2468
Asp718I GGTACC 1 cut(s) 490
AspA2I CCTAGG 1 cut(s) 197
AspLEI GCGC 4 cut(s) 640, 1021, 1905, 3186
AspS9I GGNCC 4 cut(s) 1308, 2286, 2304, 3887
AsuC2I CCSGG 4 cut(s) 35, 36, 137, 138
AsuHPI GGTGA 6 cut(s) 1052, 2288, 3032, 3181, 3857, 4150
AvaI CYCGRG 6 cut(s) 34, 104, 136, 623, 978, 3705
AvaII GGWCC 4 cut(s) 1308, 2286, 2304, 3887
AvrII CCTAGG 1 cut(s) 197
BaeGI GKGCMC 1 cut(s) 135
BaeI ACNNNNGTAYC 2 cut(s) 474, 507
BalI TGGCCA 2 cut(s) 1100, 1336
BamHI GGATCC 2 cut(s) 250, 1342
BanI GGYRCC 2 cut(s) 490, 1443
BanII GRGCYC 3 cut(s) 11, 1945, 2182
BarI GAAGNNNNNNTAC 4 cut(s) 14, 46, 3102, 3134
BauI CACGAG 2 cut(s) 3621, 3948
BbrPI CACGTG 1 cut(s) 3227
BbsI GAAGAC 5 cut(s) 29, 659, 714, 1552, 2499
Bbv12I GWGCWC 5 cut(s) 11, 480, 3232, 3472, 3685
BbvCI CCTCAGC 1 cut(s) 2418
BbvI GCAGC 7 cut(s) 647, 1175, 1178, 1375, 2511, 2812, 2823
BccI CCATC 9 cut(s) 401, 430, 520, 901, 1090, 1266, 3011, 3158, 3323
BceAI ACGGC 3 cut(s) 1272, 1847, 2335
BcgI CGANNNNNNTGC 2 cut(s) 2969, 3003
BciT130I CCWGG 7 cut(s) 937, 1496, 2192, 3539, 3617, 3885, 4124
BclI TGATCA 1 cut(s) 2970
BcnI CCSGG 4 cut(s) 35, 36, 137, 138
BcoDI GTCTC 3 cut(s) 320, 2699, 3109
BfaI CTAG 9 cut(s) 198, 1328, 1740, 1791, 2586, 3483, 3521, 3833, 4164
BfmI CTRYAG 8 cut(s) 726, 1450, 1698, 1758, 3353, 3508, 3894, 3962
BfoI RGCGCY 2 cut(s) 641, 1906
BfrI CTTAAG 2 cut(s) 3053, 3589
BfuAI ACCTGC 1 cut(s) 3360
BglII AGATCT 2 cut(s) 347, 2518
BlnI CCTAGG 1 cut(s) 197
BlpI GCTNAGC 1 cut(s) 1944
BmcAI AGTACT 1 cut(s) 465
Bme18I GGWCC 4 cut(s) 1308, 2286, 2304, 3887
BmeRI GACNNNNNGTC 1 cut(s) 115
BmeT110I CYCGRG 6 cut(s) 34, 104, 136, 623, 978, 3705
BmgBI CACGTC 1 cut(s) 269
BmgT120I GGNCC 4 cut(s) 1308, 2286, 2304, 3887
BmiI GGNNCC 8 cut(s) 252, 492, 974, 1344, 1445, 2181, 2288, 4204
BoxI GACNNNNGTC 1 cut(s) 2558
BpiI GAAGAC 5 cut(s) 29, 659, 714, 1552, 2499
BpmI CTGGAG 2 cut(s) 464, 3017
Bpu10I CCTNAGC 1 cut(s) 2418
Bpu1102I GCTNAGC 1 cut(s) 1944
BpuMI CCSGG 4 cut(s) 35, 36, 137, 138
BsaAI YACGTR 2 cut(s) 3227, 3764
BsaBI GATNNNNATC 2 cut(s) 2517, 4150
BsaHI GRCGYC 1 cut(s) 116
BsaWI WCCGGW 2 cut(s) 427, 1145
BsaXI ACNNNNNCTCC 2 cut(s) 3026, 3056
Bsc4I CCNNNNNNNGG 6 cut(s) 487, 1096, 1145, 3453, 3454, 4129
Bse1I ACTGG 3 cut(s) 2563, 3000, 4102
Bse3DI GCAATG 3 cut(s) 1014, 1135, 2166
Bse8I GATNNNNATC 2 cut(s) 2517, 4150
BseBI CCWGG 7 cut(s) 937, 1496, 2192, 3539, 3617, 3885, 4124
BseJI GATNNNNATC 2 cut(s) 2517, 4150
BseLI CCNNNNNNNGG 6 cut(s) 487, 1096, 1145, 3453, 3454, 4129
BseMI GCAATG 3 cut(s) 1014, 1135, 2166
BseMII CTCAG 8 cut(s) 867, 1958, 2409, 2697, 2725, 3095, 3414, 3698
BseNI ACTGG 3 cut(s) 2563, 3000, 4102
BseRI GAGGAG 2 cut(s) 200, 3803
BseSI GKGCMC 1 cut(s) 135
BseXI GCAGC 7 cut(s) 647, 1175, 1178, 1375, 2511, 2812, 2823
BsgI GTGCAG 1 cut(s) 1769
Bsh1236I CGCG 2 cut(s) 49, 3243
Bsh1285I CGRYCG 3 cut(s) 53, 1465, 2489
BshFI GGCC 6 cut(s) 1100, 1336, 1378, 1859, 2569, 2730
BshNI GGYRCC 2 cut(s) 490, 1443
BsiEI CGRYCG 3 cut(s) 53, 1465, 2489
BsiHKAI GWGCWC 5 cut(s) 11, 480, 3232, 3472, 3685
BsiHKCI CYCGRG 6 cut(s) 34, 104, 136, 623, 978, 3705
BsiSI CCGG 5 cut(s) 35, 137, 428, 1139, 1146
BslFI GGGAC 2 cut(s) 31, 153
BslI CCNNNNNNNGG 6 cut(s) 487, 1096, 1145, 3453, 3454, 4129
BsmAI GTCTC 3 cut(s) 320, 2699, 3109
BsmFI GGGAC 2 cut(s) 31, 153
BsmI GAATGC 3 cut(s) 1076, 2261, 2702
BsnI GGCC 6 cut(s) 1100, 1336, 1378, 1859, 2569, 2730
BsoBI CYCGRG 6 cut(s) 34, 104, 136, 623, 978, 3705
Bsp1286I GDGCHC 8 cut(s) 11, 135, 480, 1945, 2182, 3232, 3472, 3685
Bsp1407I TGTACA 3 cut(s) 649, 688, 1224
Bsp1720I GCTNAGC 1 cut(s) 1944
Bsp19I CCATGG 1 cut(s) 223
Bsp68I TCGCGA 1 cut(s) 3243
BspANI GGCC 6 cut(s) 1100, 1336, 1378, 1859, 2569, 2730
BspCNI CTCAG 8 cut(s) 868, 1957, 2410, 2696, 2724, 3094, 3415, 3697
BspFNI CGCG 2 cut(s) 49, 3243
BspHI TCATGA 2 cut(s) 1975, 3213
BspLI GGNNCC 8 cut(s) 252, 492, 974, 1344, 1445, 2181, 2288, 4204
BspMAI CTGCAG 3 cut(s) 730, 3357, 3966
BspMI ACCTGC 1 cut(s) 3360
BspPI GGATC 7 cut(s) 245, 258, 347, 1337, 1350, 2024, 3398
BspQI GCTCTTC 1 cut(s) 1646
BspT107I GGYRCC 2 cut(s) 490, 1443
BspTI CTTAAG 2 cut(s) 3053, 3589
BsrDI GCAATG 3 cut(s) 1014, 1135, 2166
BsrGI TGTACA 3 cut(s) 649, 688, 1224
BsrI ACTGG 3 cut(s) 2563, 3000, 4102
BssNI GRCGYC 1 cut(s) 116
BssSI CACGAG 2 cut(s) 3621, 3948
BssT1I CCWWGG 3 cut(s) 197, 223, 2290
Bst2BI CACGAG 2 cut(s) 3621, 3948
Bst2UI CCWGG 7 cut(s) 937, 1496, 2192, 3539, 3617, 3885, 4124
Bst6I CTCTTC 6 cut(s) 426, 1173, 1646, 1960, 2733, 2739
BstACI GRCGYC 1 cut(s) 116
BstAFI CTTAAG 2 cut(s) 3053, 3589
BstAUI TGTACA 3 cut(s) 649, 688, 1224
BstBAI YACGTR 2 cut(s) 3227, 3764
BstDSI CCRYGG 3 cut(s) 47, 223, 891
BstFNI CGCG 2 cut(s) 49, 3243
BstH2I RGCGCY 2 cut(s) 641, 1906
BstHHI GCGC 4 cut(s) 640, 1021, 1905, 3186
BstMAI GTCTC 3 cut(s) 320, 2699, 3109
BstMCI CGRYCG 3 cut(s) 53, 1465, 2489
BstMWI GCNNNNNNNGC 8 cut(s) 298, 644, 1333, 1496, 1575, 1900, 2177, 3617
BstNI CCWGG 7 cut(s) 937, 1496, 2192, 3539, 3617, 3885, 4124
BstNSI RCATGY 4 cut(s) 100, 2144, 3223, 3250
BstPAI GACNNNNGTC 1 cut(s) 2558
BstSFI CTRYAG 8 cut(s) 726, 1450, 1698, 1758, 3353, 3508, 3894, 3962
BstSLI GKGCMC 1 cut(s) 135
BstSNI TACGTA 1 cut(s) 3764
BstUI CGCG 2 cut(s) 49, 3243
BstV1I GCAGC 7 cut(s) 647, 1175, 1178, 1375, 2511, 2812, 2823
BstV2I GAAGAC 5 cut(s) 29, 659, 714, 1552, 2499
BstX2I RGATCY 4 cut(s) 250, 347, 1342, 2518
BstYI RGATCY 4 cut(s) 250, 347, 1342, 2518
BsuRI GGCC 6 cut(s) 1100, 1336, 1378, 1859, 2569, 2730
BtgI CCRYGG 3 cut(s) 47, 223, 891
BtgZI GCGATG 1 cut(s) 2994
BtrI CACGTC 1 cut(s) 269
BtsI GCAGTG 1 cut(s) 1991
BtsIMutI CAGTG 8 cut(s) 492, 873, 1991, 2570, 3458, 3495, 3536, 4095
BtuMI TCGCGA 1 cut(s) 3243
BveI ACCTGC 1 cut(s) 3360
CciI TCATGA 2 cut(s) 1975, 3213
CfoI GCGC 4 cut(s) 640, 1021, 1905, 3186
Cfr13I GGNCC 4 cut(s) 1308, 2286, 2304, 3887
Cfr42I CCGCGG 1 cut(s) 50
Cfr9I CCCGGG 2 cut(s) 34, 136
CseI GACGC 4 cut(s) 105, 298, 2903, 3523
DraI TTTAAA 1 cut(s) 922
DrdI GACNNNNNNGTC 1 cut(s) 76
DriI GACNNNNNGTC 1 cut(s) 115
DseDI GACNNNNNNGTC 1 cut(s) 76
EaeI YGGCCR 3 cut(s) 1098, 1334, 1857
Eam1104I CTCTTC 6 cut(s) 426, 1173, 1646, 1960, 2733, 2739
Eam1105I GACNNNNNGTC 1 cut(s) 115
EarI CTCTTC 6 cut(s) 426, 1173, 1646, 1960, 2733, 2739
EciI GGCGGA 1 cut(s) 1816
Ecl136II GAGCTC 1 cut(s) 9
Eco105I TACGTA 1 cut(s) 3764
Eco130I CCWWGG 3 cut(s) 197, 223, 2290
Eco24I GRGCYC 3 cut(s) 11, 1945, 2182
Eco47I GGWCC 4 cut(s) 1308, 2286, 2304, 3887
Eco47III AGCGCT 2 cut(s) 639, 1904
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 8 cut(s) 389, 552, 726, 1546, 1762, 2907, 3512, 3755
Eco72I CACGTG 1 cut(s) 3227
Eco88I CYCGRG 6 cut(s) 34, 104, 136, 623, 978, 3705
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 3887
EcoRI GAATTC 1 cut(s) 4031
EcoRII CCWGG 7 cut(s) 935, 1494, 2190, 3537, 3615, 3883, 4122
EcoT14I CCWWGG 3 cut(s) 197, 223, 2290
EcoT38I GRGCYC 3 cut(s) 11, 1945, 2182
ErhI CCWWGG 3 cut(s) 197, 223, 2290
FalI AAGNNNNNCTT 2 cut(s) 624, 656
FaqI GGGAC 2 cut(s) 31, 153
FauI CCCGC 1 cut(s) 8
FauNDI CATATG 2 cut(s) 1034, 2771
FbaI TGATCA 1 cut(s) 2970
FblI GTMKAC 1 cut(s) 2784
FriOI GRGCYC 3 cut(s) 11, 1945, 2182
FspBI CTAG 9 cut(s) 198, 1328, 1740, 1791, 2586, 3483, 3521, 3833, 4164
GlaI GCGC 4 cut(s) 639, 1020, 1904, 3185
GsuI CTGGAG 2 cut(s) 464, 3017
HaeII RGCGCY 2 cut(s) 641, 1906
HaeIII GGCC 6 cut(s) 1100, 1336, 1378, 1859, 2569, 2730
HapII CCGG 5 cut(s) 35, 137, 428, 1139, 1146
HgaI GACGC 4 cut(s) 105, 298, 2903, 3523
HhaI GCGC 4 cut(s) 640, 1021, 1905, 3186
Hin1I GRCGYC 1 cut(s) 116
Hin6I GCGC 4 cut(s) 638, 1019, 1903, 3184
HinP1I GCGC 4 cut(s) 638, 1019, 1903, 3184
HincII GTYRAC 3 cut(s) 565, 717, 3444
HindII GTYRAC 3 cut(s) 565, 717, 3444
HindIII AAGCTT 3 cut(s) 1044, 3050, 4002
HpaI GTTAAC 1 cut(s) 3444
HpaII CCGG 5 cut(s) 35, 137, 428, 1139, 1146
HphI GGTGA 6 cut(s) 1052, 2288, 3032, 3181, 3857, 4150
Hpy166II GTNNAC 8 cut(s) 81, 266, 417, 565, 717, 2785, 3444, 3651
Hpy8I GTNNAC 8 cut(s) 81, 266, 417, 565, 717, 2785, 3444, 3651
Hpy99I CGWCG 2 cut(s) 121, 4050
HpyCH4IV ACGT 7 cut(s) 268, 901, 3027, 3070, 3226, 3749, 3763
HpyF10VI GCNNNNNNNGC 8 cut(s) 298, 644, 1333, 1496, 1575, 1900, 2177, 3617
HpySE526I ACGT 7 cut(s) 268, 901, 3027, 3070, 3226, 3749, 3763
Hsp92I GRCGYC 1 cut(s) 116
HspAI GCGC 4 cut(s) 638, 1019, 1903, 3184
KpnI GGTACC 1 cut(s) 494
Ksp22I TGATCA 1 cut(s) 2970
KspAI GTTAAC 1 cut(s) 3444
KspI CCGCGG 1 cut(s) 50
LguI GCTCTTC 1 cut(s) 1646
Lsp1109I GCAGC 7 cut(s) 647, 1175, 1178, 1375, 2511, 2812, 2823
MaeI CTAG 9 cut(s) 198, 1328, 1740, 1791, 2586, 3483, 3521, 3833, 4164
MaeII ACGT 7 cut(s) 268, 901, 3027, 3070, 3226, 3749, 3763
MaeIII GTNAC 6 cut(s) 1201, 1608, 1921, 2143, 2551, 3845
MfeI CAATTG 5 cut(s) 150, 998, 2184, 2384, 4192
MflI RGATCY 4 cut(s) 250, 347, 1342, 2518
MhlI GDGCHC 8 cut(s) 11, 135, 480, 1945, 2182, 3232, 3472, 3685
MlsI TGGCCA 2 cut(s) 1100, 1336
MluNI TGGCCA 2 cut(s) 1100, 1336
MlyI GAGTC 6 cut(s) 177, 1190, 1528, 2309, 3152, 3262
MmeI TCCRAC 5 cut(s) 99, 278, 1335, 1628, 2838
Mox20I TGGCCA 2 cut(s) 1100, 1336
MroXI GAANNNNTTC 2 cut(s) 1775, 2468
MscI TGGCCA 2 cut(s) 1100, 1336
MslI CAYNNNNRTG 7 cut(s) 128, 2215, 2274, 3012, 3155, 3218, 4047
Msp20I TGGCCA 2 cut(s) 1100, 1336
MspA1I CMGCKG 3 cut(s) 15, 49, 2803
MspCI CTTAAG 2 cut(s) 3053, 3589
MspI CCGG 5 cut(s) 35, 137, 428, 1139, 1146
MunI CAATTG 5 cut(s) 150, 998, 2184, 2384, 4192
Mva1269I GAATGC 3 cut(s) 1076, 2261, 2702
MvaI CCWGG 7 cut(s) 937, 1496, 2192, 3539, 3617, 3885, 4124
MvnI CGCG 2 cut(s) 49, 3243
MwoI GCNNNNNNNGC 8 cut(s) 298, 644, 1333, 1496, 1575, 1900, 2177, 3617
NciI CCSGG 4 cut(s) 35, 36, 137, 138
NcoI CCATGG 1 cut(s) 223
NdeI CATATG 2 cut(s) 1034, 2771
NlaIV GGNNCC 8 cut(s) 252, 492, 974, 1344, 1445, 2181, 2288, 4204
NmuCI GTSAC 3 cut(s) 1921, 2551, 3845
NruI TCGCGA 1 cut(s) 3243
NspI RCATGY 4 cut(s) 100, 2144, 3223, 3250
PaeR7I CTCGAG 1 cut(s) 978
PagI TCATGA 2 cut(s) 1975, 3213
PciI ACATGT 1 cut(s) 2140
PciSI GCTCTTC 1 cut(s) 1646
PcsI WCGNNNNNNNCGW 2 cut(s) 274, 4054
PctI GAATGC 3 cut(s) 1076, 2261, 2702
PdmI GAANNNNTTC 2 cut(s) 1775, 2468
PflMI CCANNNNNTGG 2 cut(s) 487, 1096
PfoI TCCNGGA 1 cut(s) 3537
Ple19I CGATCG 2 cut(s) 1465, 2489
PleI GAGTC 6 cut(s) 176, 1189, 1527, 2309, 3151, 3262
PmaCI CACGTG 1 cut(s) 3227
PmlI CACGTG 1 cut(s) 3227
PpsI GAGTC 6 cut(s) 176, 1189, 1527, 2309, 3151, 3262
Ppu21I YACGTR 2 cut(s) 3227, 3764
PpuMI RGGWCCY 1 cut(s) 3887
PscI ACATGT 1 cut(s) 2140
PshAI GACNNNNGTC 1 cut(s) 2558
PshBI ATTAAT 2 cut(s) 126, 4148
PsiI TTATAA 1 cut(s) 1818
Psp124BI GAGCTC 1 cut(s) 11
Psp1406I AACGTT 1 cut(s) 901
Psp5II RGGWCCY 1 cut(s) 3887
Psp6I CCWGG 7 cut(s) 935, 1494, 2190, 3537, 3615, 3883, 4122
PspCI CACGTG 1 cut(s) 3227
PspGI CCWGG 7 cut(s) 935, 1494, 2190, 3537, 3615, 3883, 4122
PspN4I GGNNCC 8 cut(s) 252, 492, 974, 1344, 1445, 2181, 2288, 4204
PspPI GGNCC 4 cut(s) 1308, 2286, 2304, 3887
PspPPI RGGWCCY 1 cut(s) 3887
PstI CTGCAG 3 cut(s) 730, 3357, 3966
PsuI RGATCY 4 cut(s) 250, 347, 1342, 2518
PvuI CGATCG 2 cut(s) 1465, 2489
RruI TCGCGA 1 cut(s) 3243
RseI CAYNNNNRTG 7 cut(s) 128, 2215, 2274, 3012, 3155, 3218, 4047
SacI GAGCTC 1 cut(s) 11
SacII CCGCGG 1 cut(s) 50
SapI GCTCTTC 1 cut(s) 1646
Sau96I GGNCC 4 cut(s) 1308, 2286, 2304, 3887
ScaI AGTACT 1 cut(s) 465
SchI GAGTC 6 cut(s) 177, 1190, 1528, 2309, 3152, 3262
SduI GDGCHC 8 cut(s) 11, 135, 480, 1945, 2182, 3232, 3472, 3685
SfcI CTRYAG 8 cut(s) 726, 1450, 1698, 1758, 3353, 3508, 3894, 3962
Sfr274I CTCGAG 1 cut(s) 978
Sfr303I CCGCGG 1 cut(s) 50
SgrBI CCGCGG 1 cut(s) 50
SinI GGWCC 4 cut(s) 1308, 2286, 2304, 3887
SlaI CTCGAG 1 cut(s) 978
SmaI CCCGGG 2 cut(s) 36, 138
SmiMI CAYNNNNRTG 7 cut(s) 128, 2215, 2274, 3012, 3155, 3218, 4047
SmlI CTYRAG 3 cut(s) 978, 3053, 3589
SmoI CTYRAG 3 cut(s) 978, 3053, 3589
SnaBI TACGTA 1 cut(s) 3764
SspI AATATT 4 cut(s) 1054, 2155, 2229, 4071
SspMI CTAG 9 cut(s) 198, 1328, 1740, 1791, 2586, 3483, 3521, 3833, 4164
SstI GAGCTC 1 cut(s) 11
StyI CCWWGG 3 cut(s) 197, 223, 2290
TaiI ACGT 7 cut(s) 271, 904, 3030, 3073, 3229, 3752, 3766
TaqII GACCGA 1 cut(s) 41
TatI WGTACW 7 cut(s) 414, 463, 649, 688, 1224, 2679, 3504
TauI GCSGC 3 cut(s) 103, 219, 1862
TscAI CASTG 8 cut(s) 492, 880, 1998, 2570, 3458, 3495, 3536, 4102
TseFI GTSAC 3 cut(s) 1921, 2551, 3845
TseI GCWGC 7 cut(s) 635, 1163, 1166, 1388, 2524, 2800, 2836
Tsp45I GTSAC 3 cut(s) 1921, 2551, 3845
TspGWI ACGGA 5 cut(s) 570, 1903, 2299, 3409, 3800
TspMI CCCGGG 2 cut(s) 34, 136
TspRI CASTG 8 cut(s) 492, 880, 1998, 2570, 3458, 3495, 3536, 4102
Van91I CCANNNNNTGG 2 cut(s) 487, 1096
Vha464I CTTAAG 2 cut(s) 3053, 3589
VpaK11BI GGWCC 4 cut(s) 1308, 2286, 2304, 3887
VspI ATTAAT 2 cut(s) 126, 4148
XbaI TCTAGA 2 cut(s) 3482, 3520
XceI RCATGY 4 cut(s) 100, 2144, 3223, 3250
XcmI CCANNNNNNNNNTGG 2 cut(s) 88, 4191
XhoI CTCGAG 1 cut(s) 978
XmaI CCCGGG 2 cut(s) 34, 136
XmaJI CCTAGG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 2784
XmnI GAANNNNTTC 2 cut(s) 1775, 2468
XspI CTAG 9 cut(s) 198, 1328, 1740, 1791, 2586, 3483, 3521, 3833, 4164
ZrmI AGTACT 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.