pycom17g28130

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007718
Physical Location & Seq
Forward (+)
26161955 .. 26167044
5090 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 825 bp
ATGATAATGGAAGTGGCACAGACAGGGTGGAAATTATGGTATTCATATTCATGGGGGTTAAGAGTAGTAGGGGGAGTAGGTGGTGTTTCAAGAAGAAGCAGTGGCAGAGCAGCTGCTGCTGCTGTAACACTAAGAAGAAAGGAAAGCTTCAATAACTGGGCGTGGTCCCAAAAGCATCCACCACCGCCACAATCCTTCTTCTTCGTACGGTGCTGTAAGTACGGAAGTAGCAGCAGCAGCAGCAGTAGCAGCGTCAGTGCTAGTAATAGTAATGGGCCTAATGGCTCCAATGTCGGAAAGGGATATCTGCAATCTACGGACCAAGAGCTGATGAGCCAGTGCCAAATGGACACTTTCAAGACGTCGGGTCCGGGGGGTCAGCACCGTAACAAGCGAGAGTCTGCCGTACGCCTCAAGCATCTCCCCACCGGTATTACTGCGCAGGCTGCGGAGGACCGATCCCAGCATATGAATCGTGCCTCAGCCTTGGCTCGTCTTCGCACTCTTATAGCACTCAAAGTCAGGAACACCGTCGATCTCGATGCTTATTCCCCTCCTCGAGAGCTTCTTCAAATTCTTCCCCCAAAGTCTACCATCAGAGGCTCAGATTGTGGTCCCCAAATTGGTCCCAACAATCCTAAGTTCATTTTGGGAATGCAAGCTCTGCTGGATCTCATTTTCACAGTTGACGGTTCTGTCTCGGAGACAGCAAAGTTCCTGGGGTTGAGCACAGGTGCTCTGTCGCGGTTAATACTCTCGAATGATTCTTTCCGACTAGCAGTCAATGAACTAAGGTTTTCCAAGGGTATGAAGCCTCTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

275

Amino Acids

29.8

Weight (kDa)

10.26

Isoelectric Point (pI)

44.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RF-1 PF00472 112 - 171 2e-19 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 695
AatII GACGTC 1 cut(s) 365
Acc16I TGCGCA 1 cut(s) 441
AccI GTMKAC 1 cut(s) 590
AccII CGCG 1 cut(s) 745
AciI CCGC 3 cut(s) 185, 449, 745
AclWI GGATC 2 cut(s) 453, 678
AcsI RAATTY 1 cut(s) 573
AcyI GRCGYC 1 cut(s) 362
AfaI GTAC 3 cut(s) 207, 221, 408
AfiI CCNNNNNNNGG 1 cut(s) 623
AgeI ACCGGT 1 cut(s) 428
AgsI TTSAA 4 cut(s) 90, 151, 358, 572
AhdI GACNNNNNGTC 2 cut(s) 366, 779
AjnI CCWGG 1 cut(s) 717
AluBI AGCT 5 cut(s) 113, 147, 328, 565, 662
AluI AGCT 5 cut(s) 113, 147, 328, 565, 662
Alw21I GWGCWC 2 cut(s) 731, 739
Alw26I GTCTC 2 cut(s) 698, 703
AlwI GGATC 2 cut(s) 453, 678
AlwNI CAGNNNCTG 1 cut(s) 116
Ama87I CYCGRG 1 cut(s) 558
AoxI GGCC 1 cut(s) 275
ApoI RAATTY 1 cut(s) 573
AsiGI ACCGGT 1 cut(s) 428
AspLEI GCGC 1 cut(s) 442
AspS9I GGNCC 7 cut(s) 165, 275, 319, 368, 454, 614, 626
AsuC2I CCSGG 1 cut(s) 372
AvaI CYCGRG 1 cut(s) 558
AvaII GGWCC 6 cut(s) 165, 319, 368, 454, 614, 626
BbsI GAAGAC 1 cut(s) 488
Bbv12I GWGCWC 2 cut(s) 731, 739
BbvCI CCTCAGC 1 cut(s) 481
BccI CCATC 1 cut(s) 602
BceAI ACGGC 1 cut(s) 389
BcgI CGANNNNNNTGC 4 cut(s) 455, 489, 524, 558
BciT130I CCWGG 1 cut(s) 719
BcnI CCSGG 1 cut(s) 372
BcoDI GTCTC 2 cut(s) 698, 703
BfaI CTAG 2 cut(s) 261, 776
Bme1390I CCNGG 2 cut(s) 372, 719
Bme18I GGWCC 6 cut(s) 165, 319, 368, 454, 614, 626
BmeRI GACNNNNNGTC 2 cut(s) 366, 779
BmeT110I CYCGRG 1 cut(s) 558
BmgT120I GGNCC 7 cut(s) 165, 275, 319, 368, 454, 614, 626
BmiI GGNNCC 5 cut(s) 167, 286, 369, 616, 628
BmrFI CCNGG 2 cut(s) 372, 719
BmrI ACTGGG 1 cut(s) 166
BmsI GCATC 3 cut(s) 184, 427, 532
BmuI ACTGGG 1 cut(s) 166
BpiI GAAGAC 1 cut(s) 488
Bpu10I CCTNAGC 1 cut(s) 481
BpuEI CTTGAG 2 cut(s) 398, 803
BpuMI CCSGG 1 cut(s) 372
BsaHI GRCGYC 1 cut(s) 362
BsaJI CCNNGG 4 cut(s) 371, 486, 718, 801
BsaWI WCCGGW 1 cut(s) 428
Bsc4I CCNNNNNNNGG 1 cut(s) 623
Bse118I RCCGGY 1 cut(s) 428
Bse1I ACTGG 2 cut(s) 161, 337
BseBI CCWGG 1 cut(s) 719
BseDI CCNNGG 4 cut(s) 371, 486, 718, 801
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 1 cut(s) 623
BseMII CTCAG 2 cut(s) 495, 618
BseNI ACTGG 2 cut(s) 161, 337
BseRI GAGGAG 1 cut(s) 546
BseYI CCCAGC 1 cut(s) 462
Bsh1236I CGCG 1 cut(s) 745
BshFI GGCC 1 cut(s) 277
BshTI ACCGGT 1 cut(s) 428
BsiHKAI GWGCWC 2 cut(s) 731, 739
BsiHKCI CYCGRG 1 cut(s) 558
BsiSI CCGG 2 cut(s) 371, 429
BsiWI CGTACG 2 cut(s) 205, 406
BslFI GGGAC 3 cut(s) 151, 600, 612
BslI CCNNNNNNNGG 1 cut(s) 623
BsmAI GTCTC 2 cut(s) 698, 703
BsmFI GGGAC 3 cut(s) 151, 600, 612
BsmI GAATGC 1 cut(s) 660
BsnI GGCC 1 cut(s) 277
BsoBI CYCGRG 1 cut(s) 558
Bsp1286I GDGCHC 2 cut(s) 731, 739
Bsp143I GATC 3 cut(s) 458, 535, 670
BspACI CCGC 3 cut(s) 185, 449, 745
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 2 cut(s) 494, 617
BspFNI CGCG 1 cut(s) 745
BspLI GGNNCC 5 cut(s) 167, 286, 369, 616, 628
BspPI GGATC 2 cut(s) 453, 678
BsrFI RCCGGY 1 cut(s) 428
BsrI ACTGG 2 cut(s) 161, 337
BssAI RCCGGY 1 cut(s) 428
BssECI CCNNGG 4 cut(s) 371, 486, 718, 801
BssMI GATC 3 cut(s) 458, 535, 670
BssNI GRCGYC 1 cut(s) 362
BssT1I CCWWGG 2 cut(s) 486, 801
Bst2UI CCWGG 1 cut(s) 719
Bst4CI ACNGT 5 cut(s) 210, 386, 532, 685, 692
BstACI GRCGYC 1 cut(s) 362
BstAPI GCANNNNNTGC 2 cut(s) 116, 664
BstC8I GCNNGC 2 cut(s) 444, 660
BstDEI CTNAG 5 cut(s) 131, 481, 604, 639, 791
BstF5I GGATG 1 cut(s) 175
BstFNI CGCG 1 cut(s) 745
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 3 cut(s) 461, 538, 673
BstMAI GTCTC 2 cut(s) 698, 703
BstMBI GATC 3 cut(s) 458, 535, 670
BstMWI GCNNNNNNNGC 8 cut(s) 116, 119, 237, 240, 246, 249, 446, 664
BstNI CCWGG 1 cut(s) 719
BstSCI CCNGG 2 cut(s) 370, 717
BstUI CGCG 1 cut(s) 745
BstV2I GAAGAC 1 cut(s) 488
BstX2I RGATCY 1 cut(s) 670
BstYI RGATCY 1 cut(s) 670
BsuRI GGCC 1 cut(s) 277
BtsCI GGATG 1 cut(s) 175
BtsI GCAGTG 1 cut(s) 106
BtsIMutI CAGTG 3 cut(s) 106, 262, 344
Cac8I GCNNGC 2 cut(s) 444, 660
CaiI CAGNNNCTG 1 cut(s) 116
CfoI GCGC 1 cut(s) 442
Cfr10I RCCGGY 1 cut(s) 428
Cfr13I GGNCC 7 cut(s) 165, 275, 319, 368, 454, 614, 626
CseI GACGC 1 cut(s) 241
Csp6I GTAC 3 cut(s) 206, 220, 407
CspAI ACCGGT 1 cut(s) 428
CviAII CATG 1 cut(s) 51
CviQI GTAC 3 cut(s) 206, 220, 407
DdeI CTNAG 5 cut(s) 131, 481, 604, 639, 791
DpnI GATC 3 cut(s) 460, 537, 672
DpnII GATC 3 cut(s) 458, 535, 670
DrdI GACNNNNNNGTC 1 cut(s) 695
DriI GACNNNNNGTC 2 cut(s) 366, 779
DseDI GACNNNNNNGTC 1 cut(s) 695
Eam1105I GACNNNNNGTC 2 cut(s) 366, 779
Eco130I CCWWGG 2 cut(s) 486, 801
Eco32I GATATC 1 cut(s) 305
Eco47I GGWCC 6 cut(s) 165, 319, 368, 454, 614, 626
Eco88I CYCGRG 1 cut(s) 558
EcoRII CCWGG 1 cut(s) 717
EcoRV GATATC 1 cut(s) 305
EcoT14I CCWWGG 2 cut(s) 486, 801
ErhI CCWWGG 2 cut(s) 486, 801
FaeI CATG 1 cut(s) 54
FaiI YATR 7 cut(s) 37, 46, 52, 468, 470, 509, 809
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FaqI GGGAC 3 cut(s) 151, 600, 612
FatI CATG 1 cut(s) 50
FauNDI CATATG 1 cut(s) 468
FblI GTMKAC 1 cut(s) 590
FokI GGATG 1 cut(s) 162
FspBI CTAG 2 cut(s) 261, 776
FspI TGCGCA 1 cut(s) 441
GlaI GCGC 1 cut(s) 441
GsaI CCCAGC 1 cut(s) 466
HaeIII GGCC 1 cut(s) 277
HapII CCGG 2 cut(s) 371, 429
HgaI GACGC 1 cut(s) 241
HhaI GCGC 1 cut(s) 442
Hin1I GRCGYC 1 cut(s) 362
Hin1II CATG 1 cut(s) 54
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HincII GTYRAC 1 cut(s) 688
HindII GTYRAC 1 cut(s) 688
HindIII AAGCTT 1 cut(s) 145
HinfI GANTC 3 cut(s) 398, 472, 764
HpaII CCGG 2 cut(s) 371, 429
Hpy166II GTNNAC 2 cut(s) 591, 688
Hpy188I TCNGA 5 cut(s) 296, 599, 607, 703, 773
Hpy188III TCNNGA 6 cut(s) 90, 358, 523, 539, 560, 757
Hpy8I GTNNAC 2 cut(s) 591, 688
Hpy99I CGWCG 2 cut(s) 367, 536
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 5 cut(s) 210, 386, 532, 685, 692
HpyCH4IV ACGT 1 cut(s) 362
HpyCH4V TGCA 2 cut(s) 310, 658
HpyF10VI GCNNNNNNNGC 8 cut(s) 116, 119, 237, 240, 246, 249, 446, 664
HpyF3I CTNAG 5 cut(s) 131, 481, 604, 639, 791
HpySE526I ACGT 1 cut(s) 362
Hsp92I GRCGYC 1 cut(s) 362
Hsp92II CATG 1 cut(s) 54
HspAI GCGC 1 cut(s) 440
Kzo9I GATC 3 cut(s) 458, 535, 670
LmnI GCTCC 1 cut(s) 290
LweI GCATC 3 cut(s) 184, 427, 532
MaeI CTAG 2 cut(s) 261, 776
MaeII ACGT 1 cut(s) 362
MaeIII GTNAC 2 cut(s) 124, 386
MalI GATC 3 cut(s) 460, 537, 672
MboI GATC 3 cut(s) 458, 535, 670
MboII GAAGA 7 cut(s) 105, 147, 190, 193, 488, 560, 569
MflI RGATCY 1 cut(s) 670
MhlI GDGCHC 2 cut(s) 731, 739
MluCI AATT 3 cut(s) 32, 573, 621
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 2 cut(s) 274, 796
MnlI CCTC 7 cut(s) 422, 445, 490, 564, 567, 593, 825
MseI TTAA 2 cut(s) 59, 749
MslI CAYNNNNRTG 1 cut(s) 49
MspA1I CMGCKG 1 cut(s) 113
MspI CCGG 2 cut(s) 371, 429
MspR9I CCNGG 2 cut(s) 372, 719
Mva1269I GAATGC 1 cut(s) 660
MvaI CCWGG 1 cut(s) 719
MvnI CGCG 1 cut(s) 745
MwoI GCNNNNNNNGC 8 cut(s) 116, 119, 237, 240, 246, 249, 446, 664
NciI CCSGG 1 cut(s) 372
NdeI CATATG 1 cut(s) 468
NdeII GATC 3 cut(s) 458, 535, 670
NlaIII CATG 1 cut(s) 54
NlaIV GGNNCC 5 cut(s) 167, 286, 369, 616, 628
NsbI TGCGCA 1 cut(s) 441
PaeR7I CTCGAG 1 cut(s) 558
PctI GAATGC 1 cut(s) 660
PfeI GAWTC 2 cut(s) 472, 764
Pfl23II CGTACG 2 cut(s) 205, 406
PinAI ACCGGT 1 cut(s) 428
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
Psp6I CCWGG 1 cut(s) 717
PspFI CCCAGC 1 cut(s) 462
PspGI CCWGG 1 cut(s) 717
PspLI CGTACG 2 cut(s) 205, 406
PspN4I GGNNCC 5 cut(s) 167, 286, 369, 616, 628
PspPI GGNCC 7 cut(s) 165, 275, 319, 368, 454, 614, 626
PstNI CAGNNNCTG 1 cut(s) 116
PsuI RGATCY 1 cut(s) 670
PvuII CAGCTG 1 cut(s) 113
RsaI GTAC 3 cut(s) 207, 221, 408
RsaNI GTAC 3 cut(s) 206, 220, 407
RseI CAYNNNNRTG 1 cut(s) 49
SaqAI TTAA 2 cut(s) 59, 749
Sau3AI GATC 3 cut(s) 458, 535, 670
Sau96I GGNCC 7 cut(s) 165, 275, 319, 368, 454, 614, 626
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 2 cut(s) 372, 719
SduI GDGCHC 2 cut(s) 731, 739
SetI ASST 9 cut(s) 82, 115, 149, 330, 365, 567, 664, 736, 797
SfaNI GCATC 3 cut(s) 184, 427, 532
Sfr274I CTCGAG 1 cut(s) 558
SinI GGWCC 6 cut(s) 165, 319, 368, 454, 614, 626
SlaI CTCGAG 1 cut(s) 558
SmiMI CAYNNNNRTG 1 cut(s) 49
SmlI CTYRAG 3 cut(s) 413, 558, 818
SmoI CTYRAG 3 cut(s) 413, 558, 818
Sse9I AATT 3 cut(s) 32, 573, 621
SsiI CCGC 3 cut(s) 185, 449, 745
SspMI CTAG 2 cut(s) 261, 776
StyD4I CCNGG 2 cut(s) 370, 717
StyI CCWWGG 2 cut(s) 486, 801
TaaI ACNGT 5 cut(s) 210, 386, 532, 685, 692
TaiI ACGT 1 cut(s) 365
TaqI TCGA 4 cut(s) 534, 540, 559, 758
TaqII GACCGA 1 cut(s) 471
TasI AATT 3 cut(s) 32, 573, 621
TfiI GAWTC 2 cut(s) 472, 764
Tru1I TTAA 2 cut(s) 59, 749
Tru9I TTAA 2 cut(s) 59, 749
TscAI CASTG 3 cut(s) 106, 262, 344
TspDTI ATGAA 6 cut(s) 33, 39, 485, 634, 801, 824
TspGWI ACGGA 2 cut(s) 237, 332
TspRI CASTG 3 cut(s) 106, 262, 344
VpaK11BI GGWCC 6 cut(s) 165, 319, 368, 454, 614, 626
XapI RAATTY 1 cut(s) 573
XhoI CTCGAG 1 cut(s) 558
XmiI GTMKAC 1 cut(s) 590
XspI CTAG 2 cut(s) 261, 776
ZraI GACGTC 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.