pycom2874g00110

Protein of unknown function (DUF1308)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002874
OG0007299
Physical Location & Seq
Reverse (-)
77670 .. 80456
2787 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1353 bp
ATGGAAGTCGGAAGAAAGGAGGTGATTGATGGAGCAGGAGCAGAAGCAGACGCAGATGCATATGCATATGCAGCACAACAAGCAGCAGTGGAATTATTGGCAAGGAAGAGATGCAGATTTGTGATAGGGCGGATAGAGGGATTGCCAGCTCCTACCAACATCAAACGCACTCTTCTCAAACTGGTTCACTCCGAACTCACTTTTCTCTCCCGCTCATCCTCCGCCGCCTCCTCCTCCACTTCTTCAACTCCACTCACCAACATCAAGAGTGTCAATATCGGACACATCGAGGCAGTTGTTCACATTCTCCGACAGCCTTTCATTACTGGGGTTTCTCGCGTTTGCAAGCCAATCCCTTTCTCAACCTTAGCCCCAATTGCACAGCAAACCGACCCTTGTGTCAACCACAAACACGTCCATGTTGATATAGTTTGCACACTCCATAGGAACCCTGTCTGGATTGTTGTCTCCGACAGGAACCCTAAATACATTACTTGGACTGGCTTCCGATCCCCTTCCCATCATAAGAGTAGGGGCTTAAAATTGCGTATTCAGCAACTCACTGATGCTGCCCGCTCTGCTGTGGCACTCAAACCTTCCTCGATTATTCTTTTCTTCTCATACCGAAACGGTCTTAGCAGCGTTATTTGTGATAGACTAAAAGAGGAGTTTGGGGCAACTGAGTTTCGATTGGATTTTCCTATTTTAGACTTTGATTTTGATTTATCCGAGGAACCTGGTGACTGGACTAATGTACTTGCCGCAAGAACGTTTCAACAGGCTTGTGTATTTGAAATAAAGGTTAATGATCCAAGGGACACTGTTTTCAGTTCAGAATCTGATGTCAAGGACTCATGCCTGGGAGAGGCGGCTGCTGAGACTTACCAGGATCCGTCTGTCCCTTTGAAGCACACTCAAATTTTCCCTGTTTTCTCTTCTCTGATTTCAAGAATGGGAATTCACTCATTGGATGCAAAAAATGTGGAATATGTGAAACTGGGGCGTATGGGTGATGGTGAACTTATTAACTTTGATACAACTGCTTTGATTGCTCTTATATCGGGTATTAGTAATGGTGGTACAGACAAGTTTTTGGCTACTCCAGAAAGTGAATTGAGGCAGCGGTTTAAGGGTAACTACGAGTTTGTGATTCAACAGGTGATGTCTGAGATTCAGAATCCAATTCTTGTTGAACTTGGTCGCGCAATGTCTGGAAAGAGAGGCATAATCTGTGAAAGTGTCCTTTTGGAGTTCAAAGAGTTAGCATTGATGTGTGGTGGGCCCAATGAGAACTTGAGAGCTAGCCATTTGCTAAAGTATCTTACGTCATTGCACATACGAGGAACTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1308

Protein Analysis

451

Amino Acids

49.71

Weight (kDa)

7.63

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1308 PF07000 342 - 443 1.3e-08 Protein of unknown function (DUF1308)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 398
AccBSI CCGCTC 2 cut(s) 213, 576
AccII CGCG 2 cut(s) 339, 1203
AciI CCGC 8 cut(s) 130, 211, 222, 225, 574, 762, 869, 1123
AclI AACGTT 1 cut(s) 770
AclWI GGATC 4 cut(s) 504, 803, 884, 897
AcsI RAATTY 2 cut(s) 918, 957
AfaI GTAC 2 cut(s) 756, 1081
AfiI CCNNNNNNNGG 1 cut(s) 865
AflIII ACRYGT 1 cut(s) 412
AgsI TTSAA 8 cut(s) 246, 776, 794, 907, 948, 1154, 1193, 1255
AjiI CACGTC 1 cut(s) 415
AjnI CCWGG 3 cut(s) 736, 858, 885
AluBI AGCT 2 cut(s) 149, 1301
AluI AGCT 2 cut(s) 149, 1301
Alw26I GTCTC 2 cut(s) 472, 872
AlwI GGATC 4 cut(s) 504, 803, 884, 897
AlwNI CAGNNNCTG 1 cut(s) 839
AoxI GGCC 1 cut(s) 1280
ApaI GGGCCC 1 cut(s) 1284
ApeKI GCWGC 6 cut(s) 71, 83, 569, 639, 872, 1120
ApoI RAATTY 2 cut(s) 918, 957
ArsI GACNNNNNNTTYG 2 cut(s) 701, 733
AspLEI GCGC 1 cut(s) 1205
AspS9I GGNCC 2 cut(s) 1280, 1281
AsuHPI GGTGA 6 cut(s) 34, 247, 752, 1022, 1028, 1171
AsuNHI GCTAGC 1 cut(s) 1301
BaeGI GKGCMC 1 cut(s) 1284
BamHI GGATCC 1 cut(s) 889
BanII GRGCYC 1 cut(s) 1284
BbvI GCAGC 6 cut(s) 83, 95, 556, 651, 859, 1132
BccI CCATC 3 cut(s) 23, 528, 1007
BciT130I CCWGG 3 cut(s) 738, 860, 887
BcoDI GTCTC 2 cut(s) 472, 872
BfaI CTAG 1 cut(s) 1302
BisI GCNGC 9 cut(s) 72, 84, 225, 570, 640, 762, 870, 873, 1121
BlsI GCNGC 9 cut(s) 73, 85, 226, 571, 641, 763, 871, 874, 1122
Bme1390I CCNGG 3 cut(s) 738, 860, 887
BmgBI CACGTC 1 cut(s) 415
BmgT120I GGNCC 2 cut(s) 1280, 1281
BmiI GGNNCC 5 cut(s) 449, 479, 735, 891, 1282
BmrFI CCNGG 3 cut(s) 738, 860, 887
BmrI ACTGGG 2 cut(s) 336, 1007
BmsI GCATC 4 cut(s) 46, 101, 556, 961
BmtI GCTAGC 1 cut(s) 1305
BmuI ACTGGG 2 cut(s) 336, 1007
BpmI CTGGAG 1 cut(s) 1086
Bpu10I CCTNAGC 1 cut(s) 367
BpuEI CTTGAG 1 cut(s) 1315
BsaJI CCNNGG 3 cut(s) 729, 812, 859
Bsc4I CCNNNNNNNGG 1 cut(s) 865
Bse1I ACTGG 5 cut(s) 186, 331, 505, 749, 1002
Bse3DI GCAATG 2 cut(s) 1212, 1328
BseBI CCWGG 3 cut(s) 738, 860, 887
BseDI CCNNGG 3 cut(s) 729, 812, 859
BseGI GGATG 2 cut(s) 215, 976
BseLI CCNNNNNNNGG 1 cut(s) 865
BseMI GCAATG 2 cut(s) 1212, 1328
BseMII CTCAG 3 cut(s) 672, 867, 1158
BseNI ACTGG 5 cut(s) 186, 331, 505, 749, 1002
BseRI GAGGAG 3 cut(s) 220, 223, 680
BseSI GKGCMC 1 cut(s) 1284
BseXI GCAGC 6 cut(s) 83, 95, 556, 651, 859, 1132
Bsh1236I CGCG 2 cut(s) 339, 1203
BshFI GGCC 1 cut(s) 1282
BslFI GGGAC 2 cut(s) 830, 884
BslI CCNNNNNNNGG 1 cut(s) 865
BsmAI GTCTC 2 cut(s) 472, 872
BsmFI GGGAC 2 cut(s) 830, 884
BsnI GGCC 1 cut(s) 1282
Bsp120I GGGCCC 1 cut(s) 1280
Bsp1286I GDGCHC 1 cut(s) 1284
Bsp143I GATC 3 cut(s) 509, 808, 889
BspACI CCGC 8 cut(s) 130, 211, 222, 225, 574, 762, 869, 1123
BspANI GGCC 1 cut(s) 1282
BspCNI CTCAG 3 cut(s) 673, 868, 1159
BspFNI CGCG 2 cut(s) 339, 1203
BspLI GGNNCC 5 cut(s) 449, 479, 735, 891, 1282
BspOI GCTAGC 1 cut(s) 1305
BspPI GGATC 4 cut(s) 504, 803, 884, 897
BsrBI CCGCTC 2 cut(s) 213, 576
BsrDI GCAATG 2 cut(s) 1212, 1328
BsrI ACTGG 5 cut(s) 186, 331, 505, 749, 1002
BssECI CCNNGG 3 cut(s) 729, 812, 859
BssMI GATC 3 cut(s) 509, 808, 889
BssT1I CCWWGG 1 cut(s) 812
Bst2UI CCWGG 3 cut(s) 738, 860, 887
Bst4CI ACNGT 2 cut(s) 632, 823
Bst6I CTCTTC 3 cut(s) 101, 177, 940
BstC8I GCNNGC 4 cut(s) 147, 347, 574, 1303
BstDEI CTNAG 5 cut(s) 367, 635, 681, 876, 1167
BstENI CCTNNNNNAGG 1 cut(s) 863
BstF5I GGATG 2 cut(s) 215, 976
BstFNI CGCG 2 cut(s) 339, 1203
BstHHI GCGC 1 cut(s) 1205
BstKTI GATC 3 cut(s) 512, 811, 892
BstMAI GTCTC 2 cut(s) 472, 872
BstMBI GATC 3 cut(s) 509, 808, 889
BstMWI GCNNNNNNNGC 6 cut(s) 71, 80, 377, 553, 578, 1049
BstNI CCWGG 3 cut(s) 738, 860, 887
BstSCI CCNGG 3 cut(s) 736, 858, 885
BstSLI GKGCMC 1 cut(s) 1284
BstUI CGCG 2 cut(s) 339, 1203
BstV1I GCAGC 6 cut(s) 83, 95, 556, 651, 859, 1132
BstX2I RGATCY 1 cut(s) 889
BstYI RGATCY 1 cut(s) 889
BsuRI GGCC 1 cut(s) 1282
BtrI CACGTC 1 cut(s) 415
BtsCI GGATG 2 cut(s) 215, 976
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 3 cut(s) 93, 561, 819
Cac8I GCNNGC 4 cut(s) 147, 347, 574, 1303
CaiI CAGNNNCTG 1 cut(s) 839
CfoI GCGC 1 cut(s) 1205
Cfr13I GGNCC 2 cut(s) 1280, 1281
CseI GACGC 1 cut(s) 59
CsiI ACCWGGT 1 cut(s) 736
Csp6I GTAC 2 cut(s) 755, 1080
CspCI CAANNNNNGTGG 2 cut(s) 963, 998
CviAII CATG 2 cut(s) 419, 855
CviQI GTAC 2 cut(s) 755, 1080
DdeI CTNAG 5 cut(s) 367, 635, 681, 876, 1167
DpnI GATC 3 cut(s) 511, 810, 891
DpnII GATC 3 cut(s) 509, 808, 889
DrdI GACNNNNNNGTC 1 cut(s) 398
DseDI GACNNNNNNGTC 1 cut(s) 398
Eam1104I CTCTTC 3 cut(s) 101, 177, 940
EarI CTCTTC 3 cut(s) 101, 177, 940
EciI GGCGGA 2 cut(s) 145, 211
Eco130I CCWWGG 1 cut(s) 812
Eco24I GRGCYC 1 cut(s) 1284
EcoNI CCTNNNNNAGG 1 cut(s) 863
EcoRI GAATTC 1 cut(s) 957
EcoRII CCWGG 3 cut(s) 736, 858, 885
EcoT14I CCWWGG 1 cut(s) 812
EcoT22I ATGCAT 2 cut(s) 61, 67
EcoT38I GRGCYC 1 cut(s) 1284
ErhI CCWWGG 1 cut(s) 812
FaeI CATG 2 cut(s) 422, 858
FaqI GGGAC 2 cut(s) 830, 884
FatI CATG 2 cut(s) 418, 854
FauI CCCGC 2 cut(s) 218, 581
FauNDI CATATG 2 cut(s) 61, 67
Fnu4HI GCNGC 9 cut(s) 72, 84, 225, 570, 640, 762, 870, 873, 1121
FokI GGATG 2 cut(s) 202, 983
FriOI GRGCYC 1 cut(s) 1284
Fsp4HI GCNGC 9 cut(s) 72, 84, 225, 570, 640, 762, 870, 873, 1121
FspBI CTAG 1 cut(s) 1302
GlaI GCGC 1 cut(s) 1204
GluI GCNGC 9 cut(s) 72, 84, 225, 570, 640, 762, 870, 873, 1121
GsuI CTGGAG 1 cut(s) 1086
HaeIII GGCC 1 cut(s) 1282
HgaI GACGC 1 cut(s) 59
HhaI GCGC 1 cut(s) 1205
Hin1II CATG 2 cut(s) 422, 858
Hin6I GCGC 1 cut(s) 1203
HinP1I GCGC 1 cut(s) 1203
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 5 cut(s) 836, 851, 1150, 1171, 1177
HphI GGTGA 6 cut(s) 34, 247, 752, 1022, 1028, 1171
Hpy166II GTNNAC 4 cut(s) 187, 301, 403, 1019
Hpy188III TCNNGA 5 cut(s) 265, 457, 948, 1103, 1212
Hpy8I GTNNAC 4 cut(s) 187, 301, 403, 1019
HpyAV CCTTC 2 cut(s) 525, 606
HpyCH4III ACNGT 2 cut(s) 632, 823
HpyCH4IV ACGT 3 cut(s) 414, 770, 1325
HpyCH4V TGCA 9 cut(s) 59, 65, 71, 114, 345, 380, 435, 974, 1333
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 80, 377, 553, 578, 1049
HpyF3I CTNAG 5 cut(s) 367, 635, 681, 876, 1167
HpySE526I ACGT 3 cut(s) 414, 770, 1325
Hsp92II CATG 2 cut(s) 422, 858
HspAI GCGC 1 cut(s) 1203
Kzo9I GATC 3 cut(s) 509, 808, 889
LmnI GCTCC 3 cut(s) 32, 38, 154
Lsp1109I GCAGC 6 cut(s) 83, 95, 556, 651, 859, 1132
LweI GCATC 4 cut(s) 46, 101, 556, 961
MabI ACCWGGT 1 cut(s) 736
MaeI CTAG 1 cut(s) 1302
MaeII ACGT 3 cut(s) 414, 770, 1325
MaeIII GTNAC 2 cut(s) 740, 1133
MalI GATC 3 cut(s) 511, 810, 891
MbiI CCGCTC 2 cut(s) 213, 576
MboI GATC 3 cut(s) 509, 808, 889
MboII GAAGA 6 cut(s) 24, 118, 164, 234, 607, 927
MfeI CAATTG 1 cut(s) 375
MflI RGATCY 1 cut(s) 889
MhlI GDGCHC 1 cut(s) 1284
MluCI AATT 7 cut(s) 92, 375, 542, 918, 957, 1112, 1182
MlyI GAGTC 1 cut(s) 845
MmeI TCCRAC 2 cut(s) 334, 495
Mph1103I ATGCAT 2 cut(s) 61, 67
MseI TTAA 4 cut(s) 539, 804, 1026, 1128
MslI CAYNNNNRTG 2 cut(s) 417, 1270
MspA1I CMGCKG 1 cut(s) 1123
MspR9I CCNGG 3 cut(s) 738, 860, 887
MunI CAATTG 1 cut(s) 375
MvaI CCWGG 3 cut(s) 738, 860, 887
MvnI CGCG 2 cut(s) 339, 1203
MwoI GCNNNNNNNGC 6 cut(s) 71, 80, 377, 553, 578, 1049
NdeI CATATG 2 cut(s) 61, 67
NdeII GATC 3 cut(s) 509, 808, 889
NheI GCTAGC 1 cut(s) 1301
NlaIII CATG 2 cut(s) 422, 858
NlaIV GGNNCC 5 cut(s) 449, 479, 735, 891, 1282
NmuCI GTSAC 1 cut(s) 740
NsiI ATGCAT 2 cut(s) 61, 67
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PfeI GAWTC 4 cut(s) 836, 1150, 1171, 1177
PkrI GCNGC 9 cut(s) 73, 85, 226, 571, 641, 763, 871, 874, 1122
PleI GAGTC 1 cut(s) 845
PpsI GAGTC 1 cut(s) 845
Psp1406I AACGTT 1 cut(s) 770
Psp6I CCWGG 3 cut(s) 736, 858, 885
PspGI CCWGG 3 cut(s) 736, 858, 885
PspN4I GGNNCC 5 cut(s) 449, 479, 735, 891, 1282
PspOMI GGGCCC 1 cut(s) 1280
PspPI GGNCC 2 cut(s) 1280, 1281
PsrI GAACNNNNNNTAC 2 cut(s) 470, 502
PstNI CAGNNNCTG 1 cut(s) 839
PsuI RGATCY 1 cut(s) 889
RsaI GTAC 2 cut(s) 756, 1081
RsaNI GTAC 2 cut(s) 755, 1080
RseI CAYNNNNRTG 2 cut(s) 417, 1270
SaqAI TTAA 4 cut(s) 539, 804, 1026, 1128
SatI GCNGC 9 cut(s) 72, 84, 225, 570, 640, 762, 870, 873, 1121
Sau3AI GATC 3 cut(s) 509, 808, 889
Sau96I GGNCC 2 cut(s) 1280, 1281
SchI GAGTC 1 cut(s) 845
ScrFI CCNGG 3 cut(s) 738, 860, 887
SduI GDGCHC 1 cut(s) 1284
SexAI ACCWGGT 1 cut(s) 736
SfaNI GCATC 4 cut(s) 46, 101, 556, 961
SmiMI CAYNNNNRTG 2 cut(s) 417, 1270
SmlI CTYRAG 1 cut(s) 1294
SmoI CTYRAG 1 cut(s) 1294
Sse9I AATT 7 cut(s) 92, 375, 542, 918, 957, 1112, 1182
SsiI CCGC 8 cut(s) 130, 211, 222, 225, 574, 762, 869, 1123
SspMI CTAG 1 cut(s) 1302
StyD4I CCNGG 3 cut(s) 736, 858, 885
StyI CCWWGG 1 cut(s) 812
TaaI ACNGT 2 cut(s) 632, 823
TaiI ACGT 3 cut(s) 417, 773, 1328
TaqI TCGA 3 cut(s) 288, 602, 688
TasI AATT 7 cut(s) 92, 375, 542, 918, 957, 1112, 1182
TatI WGTACW 1 cut(s) 754
TauI GCSGC 3 cut(s) 227, 764, 872
TfiI GAWTC 4 cut(s) 836, 1150, 1171, 1177
Tru1I TTAA 4 cut(s) 539, 804, 1026, 1128
Tru9I TTAA 4 cut(s) 539, 804, 1026, 1128
TscAI CASTG 3 cut(s) 93, 568, 826
TseFI GTSAC 1 cut(s) 740
TseI GCWGC 6 cut(s) 71, 83, 569, 639, 872, 1120
Tsp45I GTSAC 1 cut(s) 740
TspDTI ATGAA 1 cut(s) 310
TspGWI ACGGA 1 cut(s) 882
TspRI CASTG 3 cut(s) 93, 568, 826
XagI CCTNNNNNAGG 1 cut(s) 863
XapI RAATTY 2 cut(s) 918, 957
XspI CTAG 1 cut(s) 1302
Zsp2I ATGCAT 2 cut(s) 61, 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.