pycom290g00220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_290
OG0006537
Physical Location & Seq
Forward (+)
149332 .. 150211
880 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 444 bp
ATGGCAACACCCTTGCCTTTGAGGCTCTTCCAGCCTCTCACCTCCATTACTCCCTATCCTTCTTCTTCACACTTCCCCTGCAAGTCCTTCAAATCCTTTCACTCTGCAAGCCCTAATTTCAATCTTTCTTCTCTCTCATCTCACAACCACTCATGTACTCGACAAAGGACAACCACTCACTCCGAACGGAAGTGGCTTTGCGGACACTTGAGAAGAGATCAATATATAGATAGTCGATCCAATGAAGACGCCACAGGTGCGAGTATGTTTGGTTCCGATGAGGAAGTAGGCACCCAAATCCCAACCCAGGCTCAATCTGTTGTCGAAGGATCAGGGGCAGTTATGGTTTCAGAGTTTAGACCAGTGGCCGATGTCGATTATCTACAGGTGCGTGATTATTCTTCAGTTTGGCTTCTTTCCATTTTGTTGACTCAATGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

16.4

Weight (kDa)

6.57

Isoelectric Point (pI)

51.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 290
AciI CCGC 1 cut(s) 201
AclWI GGATC 2 cut(s) 231, 337
AcoI YGGCCR 1 cut(s) 366
AcuI CTGAAG 1 cut(s) 387
AcyI GRCGYC 1 cut(s) 249
AfaI GTAC 1 cut(s) 157
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 2 cut(s) 91, 121
AjnI CCWGG 1 cut(s) 306
AlwI GGATC 2 cut(s) 231, 337
AoxI GGCC 1 cut(s) 366
AsuHPI GGTGA 1 cut(s) 31
BanI GGYRCC 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 252
BciT130I CCWGG 1 cut(s) 308
BfmI CTRYAG 1 cut(s) 383
BglI GCCNNNNNGGC 1 cut(s) 22
Bme1390I CCNGG 1 cut(s) 308
BmiI GGNNCC 2 cut(s) 274, 292
BmrFI CCNGG 1 cut(s) 308
BpiI GAAGAC 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 229
BsaHI GRCGYC 1 cut(s) 249
BsaJI CCNNGG 1 cut(s) 306
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse1I ACTGG 1 cut(s) 362
BseBI CCWGG 1 cut(s) 308
BseDI CCNNGG 1 cut(s) 306
BseLI CCNNNNNNNGG 1 cut(s) 307
BseNI ACTGG 1 cut(s) 362
BshFI GGCC 1 cut(s) 368
BshNI GGYRCC 1 cut(s) 290
BslI CCNNNNNNNGG 1 cut(s) 307
BsnI GGCC 1 cut(s) 368
Bsp143I GATC 3 cut(s) 217, 236, 329
BspACI CCGC 1 cut(s) 201
BspANI GGCC 1 cut(s) 368
BspLI GGNNCC 2 cut(s) 274, 292
BspPI GGATC 2 cut(s) 231, 337
BspQI GCTCTTC 1 cut(s) 32
BspT107I GGYRCC 1 cut(s) 290
BsrI ACTGG 1 cut(s) 362
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 3 cut(s) 217, 236, 329
BssNI GRCGYC 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 308
Bst6I CTCTTC 2 cut(s) 32, 208
BstACI GRCGYC 1 cut(s) 249
BstC8I GCNNGC 1 cut(s) 109
BstKTI GATC 3 cut(s) 220, 239, 332
BstMBI GATC 3 cut(s) 217, 236, 329
BstMWI GCNNNNNNNGC 3 cut(s) 22, 31, 257
BstNI CCWGG 1 cut(s) 308
BstSCI CCNGG 1 cut(s) 306
BstSFI CTRYAG 1 cut(s) 383
BstV2I GAAGAC 1 cut(s) 252
BsuRI GGCC 1 cut(s) 368
BtsIMutI CAGTG 1 cut(s) 369
Cac8I GCNNGC 1 cut(s) 109
CseI GACGC 1 cut(s) 257
Csp6I GTAC 1 cut(s) 156
CviAII CATG 1 cut(s) 153
CviJI RGCY 7 cut(s) 25, 34, 111, 196, 311, 368, 412
CviKI_1 RGCY 7 cut(s) 25, 34, 111, 196, 311, 368, 412
CviQI GTAC 1 cut(s) 156
DpnI GATC 3 cut(s) 219, 238, 331
DpnII GATC 3 cut(s) 217, 236, 329
EaeI YGGCCR 1 cut(s) 366
Eam1104I CTCTTC 2 cut(s) 32, 208
EarI CTCTTC 2 cut(s) 32, 208
Eco57I CTGAAG 1 cut(s) 387
EcoRII CCWGG 1 cut(s) 306
EcoT22I ATGCAT 1 cut(s) 440
FaeI CATG 1 cut(s) 156
FaiI YATR 7 cut(s) 154, 225, 227, 266, 344, 440, 442
FatI CATG 1 cut(s) 152
HaeIII GGCC 1 cut(s) 368
HgaI GACGC 1 cut(s) 257
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 1 cut(s) 156
HincII GTYRAC 1 cut(s) 429
HindII GTYRAC 1 cut(s) 429
HinfI GANTC 1 cut(s) 430
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 1 cut(s) 429
Hpy188I TCNGA 3 cut(s) 184, 277, 352
Hpy8I GTNNAC 1 cut(s) 429
HpyAV CCTTC 3 cut(s) 69, 97, 320
HpyCH4V TGCA 3 cut(s) 81, 107, 438
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 31, 257
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 1 cut(s) 156
Kzo9I GATC 3 cut(s) 217, 236, 329
LguI GCTCTTC 1 cut(s) 32
LpnPI CCDG 8 cut(s) 44, 91, 240, 293, 318, 320, 371, 375
MalI GATC 3 cut(s) 219, 238, 331
MboI GATC 3 cut(s) 217, 236, 329
MboII GAAGA 7 cut(s) 19, 54, 57, 120, 225, 257, 393
MluCI AATT 1 cut(s) 115
MlyI GAGTC 1 cut(s) 424
MnlI CCTC 4 cut(s) 15, 45, 52, 274
Mph1103I ATGCAT 1 cut(s) 440
MspR9I CCNGG 1 cut(s) 308
MvaI CCWGG 1 cut(s) 308
MwoI GCNNNNNNNGC 3 cut(s) 22, 31, 257
NdeII GATC 3 cut(s) 217, 236, 329
NlaIII CATG 1 cut(s) 156
NlaIV GGNNCC 2 cut(s) 274, 292
NsiI ATGCAT 1 cut(s) 440
PciSI GCTCTTC 1 cut(s) 32
PleI GAGTC 1 cut(s) 424
PpsI GAGTC 1 cut(s) 424
Psp6I CCWGG 1 cut(s) 306
PspGI CCWGG 1 cut(s) 306
PspN4I GGNNCC 2 cut(s) 274, 292
PsrI GAACNNNNNNTAC 2 cut(s) 256, 288
RsaI GTAC 1 cut(s) 157
RsaNI GTAC 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 32
Sau3AI GATC 3 cut(s) 217, 236, 329
SchI GAGTC 1 cut(s) 424
ScrFI CCNGG 1 cut(s) 308
SetI ASST 3 cut(s) 44, 259, 390
SfcI CTRYAG 1 cut(s) 383
SmlI CTYRAG 1 cut(s) 208
SmoI CTYRAG 1 cut(s) 208
Sse9I AATT 1 cut(s) 115
SsiI CCGC 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 306
TaqI TCGA 4 cut(s) 160, 235, 324, 375
TasI AATT 1 cut(s) 115
TatI WGTACW 1 cut(s) 155
TscAI CASTG 1 cut(s) 369
TspDTI ATGAA 1 cut(s) 258
TspGWI ACGGA 1 cut(s) 202
TspRI CASTG 1 cut(s) 369
Zsp2I ATGCAT 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.