pycom395g00020

ETHYLENE INSENSITIVE 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000395
OG0009800
Physical Location & Seq
Reverse (-)
39816 .. 41174
1359 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1107 bp
ATGGTAGAGTTCCATGGAGAAGTTGATACTGTAAGGGTTGGGATAGTTGTAGAAGATGAGGAAGCAGCTGAAGATCACATCAGCTACGAAGAGCTCAAGAAGCGAATGTGGAAGCATCGTATTCGCTTACAAAAGCTCAAAGAGTGGTCGTTGGCAAAGCAAGAAGTGTCTCGTCGAAAGAAAATGGCAAGATCCCAAGATTCGATACTGAAATACATGGGGAAAATCATGGAGGTATGCAAAGCAAAGGGCTTTGTGTATGGGATTGTGCCTGAGAAGGGAAAGCCAATGACGGGCTCCTCTGATAGCTTAAGAAAGTGGTGGAAAGACAAAGTGAGATTTGACCAAAACGCCCCCATTGCAATAGCCGAGCATCTGCCAGCTCTGATTTTTGAACAAGGTGAGTTAGATCATGGCTCTTATGCTCATTTGCTTCATGAATTGCAAGACACTACTTTGGCGTCTCTTCTTTCTGCTCTGATGCAGCACTGCATGCCGCCACAGCGGAGGTTCCCTCTGGAGAAGGACCAAGGGATTTCTGTAGAGCATGGCGCTCCTCCTTATCGGAAGCCCCACGATCTCGCAAAGGCATGGAAAGTGAGTGCCTTGGCTGCTGTGATCAAACACATGGCCCCTAATTCAGATAAAGTGAGAAAGCTTGTGAGGCAAAGCAAGTGTTTGCAAGATAAGATGACTGCTATTTTGTCCAAAGTGGTTAACCAAGAAGAATCATTTATACAAATTACTGAGAAATGCCTCAAAATCTCTGATCACTCAAATAAAAAGGTTAAGAATATGAAGTGGCCTGCTACTGCTAGCAATGAAAAGAGGAAGTTGATGAGTACTGATTTGGGATTAGGTGTAGTGGATTGGATCGATATGGAGCTAGACAAGATCAATCGGATTGATCACAACAATGGTGCACTTGTAATCAATGAGCAAGTTGGTGGGGACGTGTCTGGTACTAAAGTGGGAGGCTATGGATCAGCAAGCTATTGGGGAAGTGGTATTTATGATGATGATGATCTAGCTATGGATGGAGCATTTGAGATCGGAAGGGGAAACATGGATTCGATTCTTTCTTCTGAAGAGATGTTACATACATAA

Protein Analysis

369

Amino Acids

41.56

Weight (kDa)

7.72

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIN3_DNA-bd PF04873 30 - 237 2.5e-82 Ethylene insensitive 3, DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 497, 505
AclWI GGATC 3 cut(s) 186, 881, 991
AcuI CTGAAG 2 cut(s) 90, 1107
AcyI GRCGYC 1 cut(s) 461
AfaI GTAC 2 cut(s) 844, 964
AfiI CCNNNNNNNGG 2 cut(s) 278, 293
AflII CTTAAG 1 cut(s) 310
AflIII ACRYGT 1 cut(s) 954
AgsI TTSAA 1 cut(s) 395
AjiI CACGTC 1 cut(s) 955
Alw21I GWGCWC 2 cut(s) 96, 925
Alw26I GTCTC 2 cut(s) 174, 468
Alw44I GTGCAC 1 cut(s) 921
AlwI GGATC 3 cut(s) 186, 881, 991
AoxI GGCC 2 cut(s) 630, 803
ApaLI GTGCAC 1 cut(s) 921
ApeKI GCWGC 3 cut(s) 65, 484, 611
ArsI GACNNNNNNTTYG 2 cut(s) 685, 717
AspLEI GCGC 1 cut(s) 554
AspS9I GGNCC 2 cut(s) 526, 631
AsuHPI GGTGA 1 cut(s) 413
AsuNHI GCTAGC 1 cut(s) 815
AvaII GGWCC 1 cut(s) 526
BaeGI GKGCMC 1 cut(s) 925
BaeI ACNNNNGTAYC 2 cut(s) 954, 987
BanII GRGCYC 2 cut(s) 96, 299
BarI GAAGNNNNNNTAC 1 cut(s) 1080
Bbv12I GWGCWC 2 cut(s) 96, 925
BbvI GCAGC 3 cut(s) 77, 496, 598
BccI CCATC 1 cut(s) 1031
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BclI TGATCA 3 cut(s) 618, 769, 907
BcoDI GTCTC 2 cut(s) 174, 468
BfaI CTAG 3 cut(s) 816, 887, 1028
BfmI CTRYAG 1 cut(s) 540
BfoI RGCGCY 1 cut(s) 555
BfrI CTTAAG 1 cut(s) 310
BisI GCNGC 4 cut(s) 66, 485, 497, 612
BlsI GCNGC 4 cut(s) 67, 486, 498, 613
BmcAI AGTACT 1 cut(s) 844
Bme18I GGWCC 1 cut(s) 526
BmgBI CACGTC 1 cut(s) 955
BmgT120I GGNCC 2 cut(s) 526, 631
BmiI GGNNCC 3 cut(s) 298, 512, 633
BmsI GCATC 3 cut(s) 124, 382, 471
BmtI GCTAGC 1 cut(s) 819
BplI GAGNNNNNCTC 2 cut(s) 499, 531
BpmI CTGGAG 1 cut(s) 539
BpuEI CTTGAG 1 cut(s) 80
Bsa29I ATCGAT 1 cut(s) 876
BsaBI GATNNNNATC 1 cut(s) 1023
BsaHI GRCGYC 1 cut(s) 461
BsaJI CCNNGG 3 cut(s) 13, 529, 606
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 293
Bse3DI GCAATG 2 cut(s) 357, 826
Bse8I GATNNNNATC 1 cut(s) 1023
BseCI ATCGAT 1 cut(s) 876
BseDI CCNNGG 3 cut(s) 13, 529, 606
BseGI GGATG 1 cut(s) 1042
BseJI GATNNNNATC 1 cut(s) 1023
BseLI CCNNNNNNNGG 2 cut(s) 278, 293
BseMI GCAATG 2 cut(s) 357, 826
BseMII CTCAG 2 cut(s) 264, 738
BseRI GAGGAG 2 cut(s) 289, 546
BseSI GKGCMC 1 cut(s) 925
BseXI GCAGC 3 cut(s) 77, 496, 598
BshFI GGCC 2 cut(s) 632, 805
BshVI ATCGAT 1 cut(s) 876
BsiHKAI GWGCWC 2 cut(s) 96, 925
BslFI GGGAC 1 cut(s) 965
BslI CCNNNNNNNGG 2 cut(s) 278, 293
BsmAI GTCTC 2 cut(s) 174, 468
BsmBI CGTCTC 1 cut(s) 468
BsmFI GGGAC 1 cut(s) 965
BsnI GGCC 2 cut(s) 632, 805
Bsp1286I GDGCHC 3 cut(s) 96, 299, 925
Bsp19I CCATGG 1 cut(s) 13
BspACI CCGC 2 cut(s) 497, 505
BspANI GGCC 2 cut(s) 632, 805
BspCNI CTCAG 2 cut(s) 265, 739
BspDI ATCGAT 1 cut(s) 876
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 3 cut(s) 298, 512, 633
BspOI GCTAGC 1 cut(s) 819
BspPI GGATC 3 cut(s) 186, 881, 991
BspQI GCTCTTC 1 cut(s) 84
BspTI CTTAAG 1 cut(s) 310
BsrDI GCAATG 2 cut(s) 357, 826
BssECI CCNNGG 3 cut(s) 13, 529, 606
BssNI GRCGYC 1 cut(s) 461
BssT1I CCWWGG 3 cut(s) 13, 529, 606
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 3 cut(s) 84, 471, 1083
BstACI GRCGYC 1 cut(s) 461
BstAFI CTTAAG 1 cut(s) 310
BstAPI GCANNNNNTGC 1 cut(s) 493
BstC8I GCNNGC 5 cut(s) 381, 494, 807, 817, 991
BstDEI CTNAG 2 cut(s) 273, 747
BstDSI CCRYGG 1 cut(s) 13
BstF5I GGATG 1 cut(s) 1042
BstH2I RGCGCY 1 cut(s) 555
BstHHI GCGC 1 cut(s) 554
BstMAI GTCTC 2 cut(s) 174, 468
BstMWI GCNNNNNNNGC 6 cut(s) 100, 359, 493, 502, 611, 664
BstNSI RCATGY 1 cut(s) 496
BstSFI CTRYAG 1 cut(s) 540
BstSLI GKGCMC 1 cut(s) 925
BstV1I GCAGC 3 cut(s) 77, 496, 598
BstX2I RGATCY 1 cut(s) 191
BstYI RGATCY 1 cut(s) 191
Bsu15I ATCGAT 1 cut(s) 876
BsuRI GGCC 2 cut(s) 632, 805
BsuTUI ATCGAT 1 cut(s) 876
BtgI CCRYGG 1 cut(s) 13
BtrI CACGTC 1 cut(s) 955
BtsCI GGATG 1 cut(s) 1042
BtsI GCAGTG 1 cut(s) 487
BtsIMutI CAGTG 1 cut(s) 487
Cac8I GCNNGC 5 cut(s) 381, 494, 807, 817, 991
CciI TCATGA 1 cut(s) 436
CfoI GCGC 1 cut(s) 554
Cfr13I GGNCC 2 cut(s) 526, 631
ClaI ATCGAT 1 cut(s) 876
CseI GACGC 1 cut(s) 450
Csp6I GTAC 2 cut(s) 843, 963
CviQI GTAC 2 cut(s) 843, 963
DdeI CTNAG 2 cut(s) 273, 747
Eam1104I CTCTTC 3 cut(s) 84, 471, 1083
EarI CTCTTC 3 cut(s) 84, 471, 1083
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 3 cut(s) 13, 529, 606
Eco24I GRGCYC 2 cut(s) 96, 299
Eco47I GGWCC 1 cut(s) 526
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 2 cut(s) 90, 1107
EcoICRI GAGCTC 1 cut(s) 94
EcoT14I CCWWGG 3 cut(s) 13, 529, 606
EcoT38I GRGCYC 2 cut(s) 96, 299
ErhI CCWWGG 3 cut(s) 13, 529, 606
Esp3I CGTCTC 1 cut(s) 468
FaqI GGGAC 1 cut(s) 965
FbaI TGATCA 3 cut(s) 618, 769, 907
Fnu4HI GCNGC 4 cut(s) 66, 485, 497, 612
FokI GGATG 1 cut(s) 1049
FriOI GRGCYC 2 cut(s) 96, 299
Fsp4HI GCNGC 4 cut(s) 66, 485, 497, 612
FspBI CTAG 3 cut(s) 816, 887, 1028
GlaI GCGC 1 cut(s) 553
GluI GCNGC 4 cut(s) 66, 485, 497, 612
GsuI CTGGAG 1 cut(s) 539
HaeII RGCGCY 1 cut(s) 555
HaeIII GGCC 2 cut(s) 632, 805
HgaI GACGC 1 cut(s) 450
HhaI GCGC 1 cut(s) 554
Hin1I GRCGYC 1 cut(s) 461
Hin6I GCGC 1 cut(s) 552
HinP1I GCGC 1 cut(s) 552
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HindIII AAGCTT 1 cut(s) 656
HinfI GANTC 4 cut(s) 200, 728, 1070, 1075
HpaI GTTAAC 1 cut(s) 718
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 2 cut(s) 718, 923
Hpy188I TCNGA 9 cut(s) 304, 387, 480, 567, 643, 769, 903, 1055, 1087
Hpy188III TCNNGA 3 cut(s) 97, 437, 518
Hpy8I GTNNAC 2 cut(s) 718, 923
Hpy99I CGWCG 1 cut(s) 177
HpyAV CCTTC 3 cut(s) 271, 517, 1050
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4IV ACGT 1 cut(s) 954
HpyCH4V TGCA 7 cut(s) 240, 362, 445, 484, 492, 682, 923
HpyF10VI GCNNNNNNNGC 6 cut(s) 100, 359, 493, 502, 611, 664
HpyF3I CTNAG 2 cut(s) 273, 747
HpySE526I ACGT 1 cut(s) 954
Hsp92I GRCGYC 1 cut(s) 461
HspAI GCGC 1 cut(s) 552
Ksp22I TGATCA 3 cut(s) 618, 769, 907
KspAI GTTAAC 1 cut(s) 718
LguI GCTCTTC 1 cut(s) 84
LmnI GCTCC 4 cut(s) 302, 559, 883, 1040
LpnPI CCDG 5 cut(s) 285, 393, 503, 819, 945
Lsp1109I GCAGC 3 cut(s) 77, 496, 598
LweI GCATC 3 cut(s) 124, 382, 471
MaeI CTAG 3 cut(s) 816, 887, 1028
MaeII ACGT 1 cut(s) 954
MaeIII GTNAC 1 cut(s) 1095
MboII GAAGA 7 cut(s) 65, 83, 101, 458, 737, 1074, 1100
MflI RGATCY 1 cut(s) 191
MhlI GDGCHC 3 cut(s) 96, 299, 925
MluCI AATT 3 cut(s) 440, 637, 741
MseI TTAA 3 cut(s) 311, 717, 789
MslI CAYNNNNRTG 1 cut(s) 915
MspA1I CMGCKG 2 cut(s) 68, 505
MspCI CTTAAG 1 cut(s) 310
MwoI GCNNNNNNNGC 6 cut(s) 100, 359, 493, 502, 611, 664
NcoI CCATGG 1 cut(s) 13
NheI GCTAGC 1 cut(s) 815
NlaIV GGNNCC 3 cut(s) 298, 512, 633
NmeAIII GCCGAG 1 cut(s) 394
NspI RCATGY 1 cut(s) 496
PaeI GCATGC 1 cut(s) 496
PagI TCATGA 1 cut(s) 436
PciSI GCTCTTC 1 cut(s) 84
PfeI GAWTC 4 cut(s) 200, 728, 1070, 1075
PkrI GCNGC 4 cut(s) 67, 486, 498, 613
Psp124BI GAGCTC 1 cut(s) 96
PspN4I GGNNCC 3 cut(s) 298, 512, 633
PspPI GGNCC 2 cut(s) 526, 631
PsuI RGATCY 1 cut(s) 191
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 2 cut(s) 844, 964
RsaNI GTAC 2 cut(s) 843, 963
RseI CAYNNNNRTG 1 cut(s) 915
SacI GAGCTC 1 cut(s) 96
SapI GCTCTTC 1 cut(s) 84
SaqAI TTAA 3 cut(s) 311, 717, 789
SatI GCNGC 4 cut(s) 66, 485, 497, 612
Sau96I GGNCC 2 cut(s) 526, 631
ScaI AGTACT 1 cut(s) 844
SduI GDGCHC 3 cut(s) 96, 299, 925
SfaNI GCATC 3 cut(s) 124, 382, 471
SfcI CTRYAG 1 cut(s) 540
SinI GGWCC 1 cut(s) 526
SmiMI CAYNNNNRTG 1 cut(s) 915
SmlI CTYRAG 2 cut(s) 95, 310
SmoI CTYRAG 2 cut(s) 95, 310
SphI GCATGC 1 cut(s) 496
Sse9I AATT 3 cut(s) 440, 637, 741
SsiI CCGC 2 cut(s) 497, 505
SspMI CTAG 3 cut(s) 816, 887, 1028
SstI GAGCTC 1 cut(s) 96
StyI CCWWGG 3 cut(s) 13, 529, 606
TaaI ACNGT 1 cut(s) 31
TaiI ACGT 1 cut(s) 957
TaqI TCGA 4 cut(s) 175, 203, 876, 1073
TasI AATT 3 cut(s) 440, 637, 741
TatI WGTACW 1 cut(s) 842
TauI GCSGC 1 cut(s) 499
TfiI GAWTC 4 cut(s) 200, 728, 1070, 1075
Tru1I TTAA 3 cut(s) 311, 717, 789
Tru9I TTAA 3 cut(s) 311, 717, 789
TscAI CASTG 1 cut(s) 494
TseI GCWGC 3 cut(s) 65, 484, 611
TspDTI ATGAA 4 cut(s) 425, 453, 812, 837
TspRI CASTG 1 cut(s) 494
Vha464I CTTAAG 1 cut(s) 310
VneI GTGCAC 1 cut(s) 921
VpaK11BI GGWCC 1 cut(s) 526
XceI RCATGY 1 cut(s) 496
XspI CTAG 3 cut(s) 816, 887, 1028
ZrmI AGTACT 1 cut(s) 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.