Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

SuperScaffold_420

Orthogroup ID

OG0008431

Physical Location & Seq

Genomic Coordinates Reverse (-)

1913894 .. 1920120

Gene Structure 6227 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2919 bp

ATGGGGAAGAAAGTTAAGAAGAAGGCTAGAGCTCCTCCAAGGGATAAGTTGGTGGCAGCCGATTCCCCAAAAAAAGCTCCCCAACAATGCAACCCAAGTGTTAAGGATGGTGACAATGGAGTTTCAGTTGCGAAAGAGAGAAGGCCTTGTCCTCATGTTGACAAAGGTGTAAATTTGGATAAATTGTCTGCTAAAATTGGGTCTTCAGGGCCTGTTAGGTGTGAATATTGCAGGGAAGGAGCGCTTGATAGGAGGGGCGGTAAGGGAAAGGGAAAACATGGGAAGAAGAAAGGGAGCTCCTCCGTGGATTCAAACTCAGAGTCCAAAGCCATTTGGGTTTGTTTGCAGTGTGGTCATTATTCTTGCGGTGGGTTTGGACTTCCCACAACCCCTCAATGCCATGCAGTTCAGCATGCTAGGCAAACTCGTCATCCAATAGTGATCCACTTTGAAAAGCCGCAGATGCGATGGTGCTTCCCGTGCAACATGCTTATTACAATTGACAAGACAGAAGAGGATGGTGAACAAAAAGTTGTGTTTGTGGATGTTGTTAAATTGATAAAGGGTCATTCATCGGAGGAATCATTGGTTAATGCTGAGGATGTGTGGTTTGTGAGTGGCAGTTTGACAAGCGAAATCAAATCAGCTAGCAATATATCATGTGATTTGGATGGAAGAGTTGGTTATACGGTGAGAGGCTTGGTTAATCTTGGTAACACTTGCTTCTTTAATTCTGTCTTACAGAACCTTCTAGCCATGGATAGGTTGCGGGGTTACTTCTTAAATTTAGATGCATCTACTGGAGCTCTTACTGTTTCCTTGAAGAAACTCTTTACTGAAGCAAAACCAGAGTCAGGAGTTAAGAATGTGATAAACCCAAGGTCTCTTTTTGGGTGTGTTTGTTCCAAGGCTCCCCAATTTAGGGGGTATCAGCAGCAAGACAGCCATGAATTGCTTCGTTGCTTACTGGATGGATTCTGCATTGAGGAGTTGAGTATGAGGAAACGTATAAATTCTTCTCAGGAAAATGGGAATCCTTCAAATTCGGGTCCTACTTTTGTGGATACTGCATTTGGGGGCCAAATAGCAAGTACTGTTCGCTGTGTCGAATGTGGTCACTCCTCAACTGTGTACGAGTCATTTTTAGATCGATCATTGTCAGTTCCAACCAGAAAATCCCCATCTAAGACTGCCCAACCCACCTCTCGAGCAAATAAGACGAAGTTGCCGCCCAAGAGGAGTGGAAAGCTTCGGTCTAAAAATAACAAAGAGAAGAATTCTGTACCATCACCAAGTGTTCCAACCCCATCAACCAGCAGTGAGGTTTCTGAACAAAAGGAATTAGTTGTAAATAGTCTATCTGCTGTCCAAGAATCTGCAAATGATGTCTGTGAGGATGCAGCAGAGCAAACATCACCTTTGTTAGATAACTGTTCATGGTTGGATTATCTTGATTCTGGAAATATGTTAGATGACAATGATTTATCTTCACAAAATAATGATATTTCAATTTTTCAAGATTCCAAAAACAAGAATACACATCTTAATGATGCCTCTCTACTGAGTGGCTCCGAATCTAGTAATCAGGTTTTTATGCCTACCGGGGAGCCAAATGTAAAACCTGACTTTTCTTCAGTGAATTCTTGGGAGGAAGAGCTCCCATTACAGGTTCAGGATTCTGAAGTTTTGTTGCTTCCGTATAAAGCAGAGTGTTCTATCACTGAGGAAATCATGGGAGGCGAAGGTGAGGCCTCCTCCTCGGTTGTGGGTTGTGGACAAGACGACTTTGGCTTTGGGGATTTATTCAACGAACCTGAAGTTTATGGCCCACCTACAAGACCCTCTGTAGGTGAGGGTGGCACGGACACTATAGCTAGCGAGTCTGATCCAGATGAAGTTGATGATACTGATTCTCCAGTGTCTGTAGAGAGTTGTTTGGCTCATTTCATAAAGCCTGAGCTTCTTGCCAGTGAAAATGCTTGGCATTGTGAGAAATGTTCCAAAACTCTTCAACGCCAAAAGTTGGAAGAAAAGAAGCAGGTAAAATCTGCTGCTAAAGTTTTGCTAAATGGATGTGAGACTAGAACACAAGGTGATTCACTGAGTTTAAATATGGGTCCTTGGCCTGCCGATGTCAGAAGGCTTGGTAATGGGAATGTCAAAAGCAATACTGGTTGTAACCAGTTTGGTGAAAACTTGGTTTTGCATGATGGAAAAATAAATTGCTTGAGCCAAAATTGCTCAGCAATTGAGAATGATCAATCACATAAGTTGAATTCTGTTGTTCGTAGACGACAATATGAGATTAAAGATGCTCCTCCAGTGCAATCAAATACTTCAGATTGTGATCAAGTCATTGATTCTAGGGCTGATGAATCTAGTAGTGCTAGTTTCACCTCGGACACTGTACCGCAGACTAATGATGGGATATTGGATGGACATCGTGAATCAGAAAAAAGTGAGGATGAGGAAATAAATTCCAAACGTGTGAATGTGATGAGGAATGCGACTAAAAGGTGCCTCATCAACAGGGCCCCACCTATTTTGACTATTCATCTTAAAAGGTTCAGCCAAGATGCTCGTGGTCGCTTGAGTAAATTGAACGGCCATGTCAGTTTTCGAGAAAAAATTGATCTTAGGCCATATATGGATTCCAGAACTTCCCGATGGGTCACCCATCCTGGGAATGCTCTCGCGCACTACTCGCTTAACTTCGGAGTTCCTATGGAACCCGAAGCCAGTGAGCTCCCAAAAGGCCTCGTGCTAGATAGGGATGGGAATATACATATAAGCGTGATCGATGATGTGATCACTCCCCTGGGCGATGTGGGATGTCACAATCCACCCCCCTTAGGGGCCCGACGTCCTCGTCGGCACACCACGACCAGGGTTAGGTTCCACAGATGGGGAGAAGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009987 GO:0019538 GO:0019941 GO:0030163 GO:0032991 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH zf-UBP

Protein Analysis

973

Amino Acids

106.53

Weight (kDa)

6.42

Isoelectric Point (pI)

51.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UBP	PF02148	107 - 166	7.5e-13	Zn-finger in ubiquitin-hydrolases and other protein
UCH	PF00443	232 - 887	1.8e-34	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04860
fragaria_vesca	FvH4_4g16490 FvH4_4g16490
malus_domestica	MD04G1009400.v1.1 MD13G1284700.v1.1
prunus_persica	Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1
pyrus_communis	pycom04g00720 pycom420g01370
rosa_chinensis	RchiOBHm_Chr4g0415171
rosa_laevigata	RLG00000008090
rosa_multiflora	Rmu_sc0003748.1_g000031 Rmu_sc0033472.1_g000001
rosa_roxburghii	Rroxscaffold_5G00359000
rosa_rugosa	Rorug04G0132500 Rorug04G0132500
rosa_samantha	Rh4AG192800 Rh4BG190900 Rh4CG203600 Rh4DG190700
rosa_wichuraiana	Rw4G016390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2868
AatII	GACGTC	1 cut(s)	2866
Acc36I	ACCTGC	1 cut(s)	2029
AccB1I	GGYRCC	1 cut(s)	2517
AccB7I	CCANNNNNTGG	1 cut(s)	2023
AccI	GTMKAC	1 cut(s)	2290
AccII	CGCG	1 cut(s)	2696
AciI	CCGC	6 cut(s)	258, 366, 458, 769, 1229, 2411
AclWI	GGATC	2 cut(s)	436, 1880
AcoI	YGGCCR	1 cut(s)	2605
AcsI	RAATTY	8 cut(s)	172, 784, 1012, 1042, 1276, 1639, 2275, 2476
AcuI	CTGAAG	6 cut(s)	189, 858, 1617, 1701, 1836, 2322
AcyI	GRCGYC	1 cut(s)	2863
AdeI	CACNNNGTG	2 cut(s)	1295, 2093
AfaI	GTAC	4 cut(s)	1093, 1133, 1284, 2409
AfeI	AGCGCT	1 cut(s)	243
AflIII	ACRYGT	1 cut(s)	2485
AjnI	CCWGG	3 cut(s)	2680, 2817, 2885
Alw21I	GWGCWC	5 cut(s)	34, 299, 808, 1659, 2748
Alw26I	GTCTC	2 cut(s)	888, 2072
AlwI	GGATC	2 cut(s)	436, 1880
AlwNI	CAGNNNCTG	2 cut(s)	212, 1922
Ama87I	CYCGRG	1 cut(s)	1206
Aor51HI	AGCGCT	1 cut(s)	243
ApaI	GGGCCC	2 cut(s)	2536, 2860
ApeKI	GCWGC	4 cut(s)	56, 934, 1400, 2051
ApoI	RAATTY	8 cut(s)	172, 784, 1012, 1042, 1276, 1639, 2275, 2476
ArsI	GACNNNNNNTTYG	6 cut(s)	305, 337, 836, 868, 1775, 1807
Asp700I	GAANNNNTTC	1 cut(s)	954
AspLEI	GCGC	2 cut(s)	244, 2698
AspS9I	GGNCC	9 cut(s)	209, 1049, 1078, 1826, 2117, 2532, 2533, 2856, 2857
AsuC2I	CCSGG	1 cut(s)	1603
AsuNHI	GCTAGC	2 cut(s)	647, 1874
AvaI	CYCGRG	1 cut(s)	1206
AvaII	GGWCC	2 cut(s)	1049, 2117
AxyI	CCTNAGG	1 cut(s)	2851
BaeGI	GKGCMC	2 cut(s)	2536, 2860
BanI	GGYRCC	1 cut(s)	2517
BanII	GRGCYC	7 cut(s)	34, 299, 808, 1659, 2536, 2748, 2860
BarI	GAAGNNNNNNTAC	2 cut(s)	1000, 1032
BauI	CACGAG	2 cut(s)	2580, 2759
BbsI	GAAGAC	1 cut(s)	195
Bbv12I	GWGCWC	5 cut(s)	34, 299, 808, 1659, 2748
BbvCI	CCTCAGC	1 cut(s)	597
BbvI	GCAGC	4 cut(s)	68, 946, 1412, 2038
BceAI	ACGGC	1 cut(s)	2620
BciT130I	CCWGG	3 cut(s)	2682, 2819, 2887
BciVI	GTATCC	1 cut(s)	1057
BclI	TGATCA	3 cut(s)	2257, 2347, 2808
BcnI	CCSGG	1 cut(s)	1603
BcoDI	GTCTC	2 cut(s)	888, 2072
BfmI	CTRYAG	3 cut(s)	1845, 1869, 1923
BfoI	RGCGCY	1 cut(s)	245
BfuAI	ACCTGC	1 cut(s)	2029
BfuI	GTATCC	1 cut(s)	1057
BisI	GCNGC	6 cut(s)	57, 458, 935, 1229, 1401, 2052
BlpI	GCTNAGC	1 cut(s)	2242
BlsI	GCNGC	6 cut(s)	58, 459, 936, 1230, 1402, 2053
BmcAI	AGTACT	1 cut(s)	1093
Bme1390I	CCNGG	4 cut(s)	1603, 2682, 2819, 2887
Bme18I	GGWCC	2 cut(s)	1049, 2117
BmeT110I	CYCGRG	1 cut(s)	1206
BmgT120I	GGNCC	9 cut(s)	209, 1049, 1078, 1826, 2117, 2532, 2533, 2856, 2857
BmrFI	CCNGG	4 cut(s)	1603, 2682, 2819, 2887
BmsI	GCATC	7 cut(s)	453, 781, 803, 1387, 1540, 2302, 2566
BmtI	GCTAGC	2 cut(s)	651, 1878
BpiI	GAAGAC	1 cut(s)	195
BplI	GAGNNNNNCTC	2 cut(s)	1739, 1771
BpmI	CTGGAG	3 cut(s)	822, 1899, 2304
Bpu10I	CCTNAGC	2 cut(s)	597, 1956
Bpu1102I	GCTNAGC	1 cut(s)	2242
BpuEI	CTTGAG	2 cut(s)	2248, 2611
BpuMI	CCSGG	1 cut(s)	1603
Bsa29I	ATCGAT	2 cut(s)	1150, 2799
BsaBI	GATNNNNATC	2 cut(s)	2346, 2439
BsaHI	GRCGYC	1 cut(s)	2863
BsaI	GGTCTC	1 cut(s)	888
BsaXI	ACNNNNNCTCC	6 cut(s)	111, 141, 244, 274, 1897, 1927
Bse1I	ACTGG	8 cut(s)	805, 972, 1916, 1968, 2176, 2182, 2321, 2739
Bse21I	CCTNAGG	1 cut(s)	2851
Bse8I	GATNNNNATC	2 cut(s)	2346, 2439
BseBI	CCWGG	3 cut(s)	2682, 2819, 2887
BseCI	ATCGAT	2 cut(s)	1150, 2799
BseJI	GATNNNNATC	2 cut(s)	2346, 2439
BseMII	CTCAG	8 cut(s)	330, 588, 1034, 1553, 1713, 1947, 2093, 2256
BseNI	ACTGG	8 cut(s)	805, 972, 1916, 1968, 2176, 2182, 2321, 2739
BseRI	GAGGAG	8 cut(s)	24, 289, 1001, 1111, 1252, 1744, 1747, 2307
BseSI	GKGCMC	2 cut(s)	2536, 2860
BseXI	GCAGC	4 cut(s)	68, 946, 1412, 2038
Bsh1236I	CGCG	1 cut(s)	2696
BshNI	GGYRCC	1 cut(s)	2517
BshVI	ATCGAT	2 cut(s)	1150, 2799
BsiHKAI	GWGCWC	5 cut(s)	34, 299, 808, 1659, 2748
BsiHKCI	CYCGRG	1 cut(s)	1206
BsiSI	CCGG	1 cut(s)	1602
BsmAI	GTCTC	2 cut(s)	888, 2072
BsmI	GAATGC	2 cut(s)	2509, 2692
Bso31I	GGTCTC	1 cut(s)	888
BsoBI	CYCGRG	1 cut(s)	1206
Bsp120I	GGGCCC	2 cut(s)	2532, 2856
Bsp1286I	GDGCHC	7 cut(s)	34, 299, 808, 1659, 2536, 2748, 2860
Bsp143I	GATC	9 cut(s)	441, 1147, 1151, 1885, 2257, 2347, 2632, 2796, 2808
Bsp1720I	GCTNAGC	1 cut(s)	2242
Bsp19I	CCATGG	1 cut(s)	756
BspACI	CCGC	6 cut(s)	258, 366, 458, 769, 1229, 2411
BspCNI	CTCAG	8 cut(s)	329, 589, 1033, 1554, 1714, 1948, 2094, 2255
BspDI	ATCGAT	2 cut(s)	1150, 2799
BspFNI	CGCG	1 cut(s)	2696
BspMI	ACCTGC	1 cut(s)	2029
BspOI	GCTAGC	2 cut(s)	651, 1878
BspPI	GGATC	2 cut(s)	436, 1880
BspQI	GCTCTTC	1 cut(s)	1647
BspT107I	GGYRCC	1 cut(s)	2517
BspTNI	GGTCTC	1 cut(s)	888
BsrI	ACTGG	8 cut(s)	805, 972, 1916, 1968, 2176, 2182, 2321, 2739
BssMI	GATC	9 cut(s)	441, 1147, 1151, 1885, 2257, 2347, 2632, 2796, 2808
BssNI	GRCGYC	1 cut(s)	2863
BssSI	CACGAG	2 cut(s)	2580, 2759
BssT1I	CCWWGG	5 cut(s)	38, 756, 878, 906, 2120
Bst2BI	CACGAG	2 cut(s)	2580, 2759
Bst2UI	CCWGG	3 cut(s)	2682, 2819, 2887
Bst4CI	ACNGT	6 cut(s)	691, 814, 1096, 1129, 1433, 2407
Bst6I	CTCTTC	4 cut(s)	507, 670, 1647, 2013
BstACI	GRCGYC	1 cut(s)	2863
BstC8I	GCNNGC	4 cut(s)	414, 649, 1876, 2127
BstDSI	CCRYGG	2 cut(s)	303, 756
BstEII	GGTNACC	1 cut(s)	2671
BstFNI	CGCG	1 cut(s)	2696
BstH2I	RGCGCY	1 cut(s)	245
BstHHI	GCGC	2 cut(s)	244, 2698
BstKTI	GATC	9 cut(s)	444, 1150, 1154, 1888, 2260, 2350, 2635, 2799, 2811
BstMAI	GTCTC	2 cut(s)	888, 2072
BstMBI	GATC	9 cut(s)	441, 1147, 1151, 1885, 2257, 2347, 2632, 2796, 2808
BstMWI	GCNNNNNNNGC	5 cut(s)	418, 463, 480, 2238, 2704
BstNI	CCWGG	3 cut(s)	2682, 2819, 2887
BstNSI	RCATGY	2 cut(s)	416, 490
BstPI	GGTNACC	1 cut(s)	2671
BstSCI	CCNGG	4 cut(s)	1601, 2680, 2817, 2885
BstSFI	CTRYAG	3 cut(s)	1845, 1869, 1923
BstSLI	GKGCMC	2 cut(s)	2536, 2860
BstUI	CGCG	1 cut(s)	2696
BstV1I	GCAGC	4 cut(s)	68, 946, 1412, 2038
BstV2I	GAAGAC	1 cut(s)	195
Bsu15I	ATCGAT	2 cut(s)	1150, 2799
Bsu36I	CCTNAGG	1 cut(s)	2851
BsuI	GTATCC	1 cut(s)	1057
BsuTUI	ATCGAT	2 cut(s)	1150, 2799
BtgI	CCRYGG	2 cut(s)	303, 756
BtgZI	GCGATG	2 cut(s)	481, 2838
BtsI	GCAGTG	2 cut(s)	353, 1324
BveI	ACCTGC	1 cut(s)	2029
Cac8I	GCNNGC	4 cut(s)	414, 649, 1876, 2127
CaiI	CAGNNNCTG	2 cut(s)	212, 1922
CfoI	GCGC	2 cut(s)	244, 2698
Cfr13I	GGNCC	9 cut(s)	209, 1049, 1078, 1826, 2117, 2532, 2533, 2856, 2857
ClaI	ATCGAT	2 cut(s)	1150, 2799
Csp6I	GTAC	4 cut(s)	1092, 1132, 1283, 2408
CspCI	CAANNNNNGTGG	4 cut(s)	1222, 1257, 2526, 2561
CviQI	GTAC	4 cut(s)	1092, 1132, 1283, 2408
DpnI	GATC	9 cut(s)	443, 1149, 1153, 1887, 2259, 2349, 2634, 2798, 2810
DpnII	GATC	9 cut(s)	441, 1147, 1151, 1885, 2257, 2347, 2632, 2796, 2808
DraI	TTTAAA	1 cut(s)	2109
DraIII	CACNNNGTG	2 cut(s)	1295, 2093
DrdI	GACNNNNNNGTC	1 cut(s)	2868
DseDI	GACNNNNNNGTC	1 cut(s)	2868
EaeI	YGGCCR	1 cut(s)	2605
Eam1104I	CTCTTC	4 cut(s)	507, 670, 1647, 2013
EarI	CTCTTC	4 cut(s)	507, 670, 1647, 2013
Ecl136II	GAGCTC	5 cut(s)	32, 297, 806, 1657, 2746
Eco130I	CCWWGG	5 cut(s)	38, 756, 878, 906, 2120
Eco147I	AGGCCT	3 cut(s)	145, 1751, 2757
Eco24I	GRGCYC	7 cut(s)	34, 299, 808, 1659, 2536, 2748, 2860
Eco31I	GGTCTC	1 cut(s)	888
Eco47I	GGWCC	2 cut(s)	1049, 2117
Eco47III	AGCGCT	1 cut(s)	243
Eco53kI	GAGCTC	5 cut(s)	32, 297, 806, 1657, 2746
Eco57I	CTGAAG	6 cut(s)	189, 858, 1617, 1701, 1836, 2322
Eco81I	CCTNAGG	1 cut(s)	2851
Eco88I	CYCGRG	1 cut(s)	1206
Eco91I	GGTNACC	1 cut(s)	2671
EcoICRI	GAGCTC	5 cut(s)	32, 297, 806, 1657, 2746
EcoO109I	RGGNCCY	6 cut(s)	209, 1049, 2117, 2532, 2533, 2856
EcoO65I	GGTNACC	1 cut(s)	2671
EcoRI	GAATTC	3 cut(s)	1276, 1639, 2275
EcoRII	CCWGG	3 cut(s)	2680, 2817, 2885
EcoT14I	CCWWGG	5 cut(s)	38, 756, 878, 906, 2120
EcoT22I	ATGCAT	1 cut(s)	796
EcoT38I	GRGCYC	7 cut(s)	34, 299, 808, 1659, 2536, 2748, 2860
ErhI	CCWWGG	5 cut(s)	38, 756, 878, 906, 2120
FalI	AAGNNNNNCTT	4 cut(s)	228, 260, 815, 847
FauI	CCCGC	1 cut(s)	762
FbaI	TGATCA	3 cut(s)	2257, 2347, 2808
FblI	GTMKAC	1 cut(s)	2290
Fnu4HI	GCNGC	6 cut(s)	57, 458, 935, 1229, 1401, 2052
FriOI	GRGCYC	7 cut(s)	34, 299, 808, 1659, 2536, 2748, 2860
Fsp4HI	GCNGC	6 cut(s)	57, 458, 935, 1229, 1401, 2052
GlaI	GCGC	2 cut(s)	243, 2697
GluI	GCNGC	6 cut(s)	57, 458, 935, 1229, 1401, 2052
GsuI	CTGGAG	3 cut(s)	822, 1899, 2304
HaeII	RGCGCY	1 cut(s)	245
HapII	CCGG	1 cut(s)	1602
HhaI	GCGC	2 cut(s)	244, 2698
Hin1I	GRCGYC	1 cut(s)	2863
Hin6I	GCGC	2 cut(s)	242, 2696
HinP1I	GCGC	2 cut(s)	242, 2696
HincII	GTYRAC	1 cut(s)	160
HindII	GTYRAC	1 cut(s)	160
HindIII	AAGCTT	1 cut(s)	1247
HpaII	CCGG	1 cut(s)	1602
Hpy166II	GTNNAC	5 cut(s)	160, 524, 1132, 1775, 2291
Hpy8I	GTNNAC	5 cut(s)	160, 524, 1132, 1775, 2291
Hpy99I	CGWCG	2 cut(s)	2865, 2874
HpyAV	CCTTC	7 cut(s)	16, 135, 230, 758, 1047, 1736, 2133
HpyCH4III	ACNGT	6 cut(s)	691, 814, 1096, 1129, 1433, 2407
HpyCH4IV	ACGT	3 cut(s)	1006, 2485, 2863
HpyF10VI	GCNNNNNNNGC	5 cut(s)	418, 463, 480, 2238, 2704
HpySE526I	ACGT	3 cut(s)	1006, 2485, 2863
Hsp92I	GRCGYC	1 cut(s)	2863
HspAI	GCGC	2 cut(s)	242, 2696
Ksp22I	TGATCA	3 cut(s)	2257, 2347, 2808
Kzo9I	GATC	9 cut(s)	441, 1147, 1151, 1885, 2257, 2347, 2632, 2796, 2808
LguI	GCTCTTC	1 cut(s)	1647
Lsp1109I	GCAGC	4 cut(s)	68, 946, 1412, 2038
LweI	GCATC	7 cut(s)	453, 781, 803, 1387, 1540, 2302, 2566
MaeII	ACGT	3 cut(s)	1006, 2485, 2863
MaeIII	GTNAC	7 cut(s)	110, 713, 773, 1115, 2177, 2671, 2834
MalI	GATC	9 cut(s)	443, 1149, 1153, 1887, 2259, 2349, 2634, 2798, 2810
MboI	GATC	9 cut(s)	441, 1147, 1151, 1885, 2257, 2347, 2632, 2796, 2808
MfeI	CAATTG	2 cut(s)	498, 2247
MhlI	GDGCHC	7 cut(s)	34, 299, 808, 1659, 2536, 2748, 2860
MlyI	GAGTC	4 cut(s)	329, 860, 1145, 1889
MmeI	TCCRAC	4 cut(s)	1190, 1325, 1422, 2004
Mph1103I	ATGCAT	1 cut(s)	796
MroXI	GAANNNNTTC	1 cut(s)	954
MslI	CAYNNNNRTG	1 cut(s)	1545
MspI	CCGG	1 cut(s)	1602
MspR9I	CCNGG	4 cut(s)	1603, 2682, 2819, 2887
MunI	CAATTG	2 cut(s)	498, 2247
Mva1269I	GAATGC	2 cut(s)	2509, 2692
MvaI	CCWGG	3 cut(s)	2682, 2819, 2887
MvnI	CGCG	1 cut(s)	2696
MwoI	GCNNNNNNNGC	5 cut(s)	418, 463, 480, 2238, 2704
NciI	CCSGG	1 cut(s)	1603
NcoI	CCATGG	1 cut(s)	756
NdeII	GATC	9 cut(s)	441, 1147, 1151, 1885, 2257, 2347, 2632, 2796, 2808
NheI	GCTAGC	2 cut(s)	647, 1874
NmuCI	GTSAC	4 cut(s)	110, 1115, 2671, 2834
NsiI	ATGCAT	1 cut(s)	796
NspI	RCATGY	2 cut(s)	416, 490
PaeI	GCATGC	1 cut(s)	416
PaeR7I	CTCGAG	1 cut(s)	1206
PasI	CCCWGGG	1 cut(s)	2818
PceI	AGGCCT	3 cut(s)	145, 1751, 2757
PciSI	GCTCTTC	1 cut(s)	1647
PctI	GAATGC	2 cut(s)	2509, 2692
PdmI	GAANNNNTTC	1 cut(s)	954
PflMI	CCANNNNNTGG	1 cut(s)	2023
PkrI	GCNGC	6 cut(s)	58, 459, 936, 1230, 1402, 2053
PleI	GAGTC	4 cut(s)	328, 859, 1144, 1888
PpsI	GAGTC	4 cut(s)	328, 859, 1144, 1888
PpuMI	RGGWCCY	2 cut(s)	1049, 2117
Psp124BI	GAGCTC	5 cut(s)	34, 299, 808, 1659, 2748
Psp5II	RGGWCCY	2 cut(s)	1049, 2117
Psp6I	CCWGG	3 cut(s)	2680, 2817, 2885
PspEI	GGTNACC	1 cut(s)	2671
PspGI	CCWGG	3 cut(s)	2680, 2817, 2885
PspOMI	GGGCCC	2 cut(s)	2532, 2856
PspPI	GGNCC	9 cut(s)	209, 1049, 1078, 1826, 2117, 2532, 2533, 2856, 2857
PspPPI	RGGWCCY	2 cut(s)	1049, 2117
PstNI	CAGNNNCTG	2 cut(s)	212, 1922
RsaI	GTAC	4 cut(s)	1093, 1133, 1284, 2409
RsaNI	GTAC	4 cut(s)	1092, 1132, 1283, 2408
RseI	CAYNNNNRTG	1 cut(s)	1545
SacI	GAGCTC	5 cut(s)	34, 299, 808, 1659, 2748
SapI	GCTCTTC	1 cut(s)	1647
SatI	GCNGC	6 cut(s)	57, 458, 935, 1229, 1401, 2052
Sau3AI	GATC	9 cut(s)	441, 1147, 1151, 1885, 2257, 2347, 2632, 2796, 2808
Sau96I	GGNCC	9 cut(s)	209, 1049, 1078, 1826, 2117, 2532, 2533, 2856, 2857
ScaI	AGTACT	1 cut(s)	1093
SchI	GAGTC	4 cut(s)	329, 860, 1145, 1889
ScrFI	CCNGG	4 cut(s)	1603, 2682, 2819, 2887
SduI	GDGCHC	7 cut(s)	34, 299, 808, 1659, 2536, 2748, 2860
SfaNI	GCATC	7 cut(s)	453, 781, 803, 1387, 1540, 2302, 2566
SfcI	CTRYAG	3 cut(s)	1845, 1869, 1923
Sfr274I	CTCGAG	1 cut(s)	1206
SinI	GGWCC	2 cut(s)	1049, 2117
SlaI	CTCGAG	1 cut(s)	1206
SmiMI	CAYNNNNRTG	1 cut(s)	1545
SmlI	CTYRAG	3 cut(s)	1206, 2227, 2590
SmoI	CTYRAG	3 cut(s)	1206, 2227, 2590
SphI	GCATGC	1 cut(s)	416
SseBI	AGGCCT	3 cut(s)	145, 1751, 2757
SsiI	CCGC	6 cut(s)	258, 366, 458, 769, 1229, 2411
SspI	AATATT	1 cut(s)	227
SstI	GAGCTC	5 cut(s)	34, 299, 808, 1659, 2748
StuI	AGGCCT	3 cut(s)	145, 1751, 2757
StyD4I	CCNGG	4 cut(s)	1601, 2680, 2817, 2885
StyI	CCWWGG	5 cut(s)	38, 756, 878, 906, 2120
TaaI	ACNGT	6 cut(s)	691, 814, 1096, 1129, 1433, 2407
TaiI	ACGT	3 cut(s)	1009, 2488, 2866
TaqI	TCGA	5 cut(s)	1107, 1150, 1207, 2620, 2799
TaqII	GACCGA	1 cut(s)	1242
TatI	WGTACW	1 cut(s)	1091
TauI	GCSGC	2 cut(s)	460, 1231
TseFI	GTSAC	4 cut(s)	110, 1115, 2671, 2834
TseI	GCWGC	4 cut(s)	56, 934, 1400, 2051
Tsp45I	GTSAC	4 cut(s)	110, 1115, 2671, 2834
TspDTI	ATGAA	7 cut(s)	561, 963, 1425, 1908, 1936, 2388, 2543
TspGWI	ACGGA	3 cut(s)	292, 1686, 1877
Van91I	CCANNNNNTGG	1 cut(s)	2023
VpaK11BI	GGWCC	2 cut(s)	1049, 2117
XapI	RAATTY	8 cut(s)	172, 784, 1012, 1042, 1276, 1639, 2275, 2476
XceI	RCATGY	2 cut(s)	416, 490
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2579
XhoI	CTCGAG	1 cut(s)	1206
XmiI	GTMKAC	1 cut(s)	2290
XmnI	GAANNNNTTC	1 cut(s)	954
ZraI	GACGTC	1 cut(s)	2864
ZrmI	AGTACT	1 cut(s)	1093
Zsp2I	ATGCAT	1 cut(s)	796

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.