pycom420g01380

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0005611
Physical Location & Seq
Reverse (-)
1925246 .. 1926874
1629 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1476 bp
ATGTTTTACTGTTTTGAAGATGCGCTGCAAGATCTTGCAGACTCATCTTTGTTTCATTTCTGCACATATTATGCTAATACTACTTTTTTCATTTTTGTGGATGCTCTTGTAATTTTAGATGGCATCGAAGCTTCTACAGATATGAGTTCGCCGGAAAGCATTTCACGTGTTGAGTTTAATGAACATGTTGATGAAACTAAACCAATGTATGGATCACTTACAACTACGATTAGAGTACTGGAACAGGCACTCAAAGAAGAGCATGCTGCTCGTGCTGCTCTGTATCTTGAACTAGAGGAGGAGAGAATTGATGCTGCCACCGCTGCAGATGAGGTCATGGCCATGATATTGCGCCTGCAAGAGGTGAAGGCATCAATTGAAATGGAGGCAAGGCAATACCAAAGGATGATAAAGGAAAAATCGGCTTACGATGCTGAGGAAATGAACATTCTTAAAGAGATGTTAGTTAGGAGGAAGAGGGAAAAACATTTCTTGGAGAATGAGGACCAGGTACTGAAGCGGGCAAGTGAATCCATCACTGAGAAGCCAACGCTGGAATTGGGACATAGTTCTCCTAGTTATGCGATCCCATCAACCGAGATAAAAGAGTATACTCTTAATTGTGGAAAAGAACTACCATTGCCAGATTTGGATGAAGATTCCGGTTCTTTGAAGCAGGTGGACATGCATTTGCATCCAAGCATAGACTATTCACATTTCTTAACTAGTTCTGATAAGTTCAATCGCGAGTTTCAAGAAAAGGAAGTGGTGTCTGTGTATGAGAAACCTTCTTCTCAAGGAAAAAAGCTCCAGAAACTAGAGGTATATCAACAACGCAGCCTGTCACCTAGTCCCAAGGGACTTAGTTTCCGTAAAAGTACTAGCCCTCAGCATAAGCAGGAACTCAATTTAGCTGCTAGCACTAGCTTTTGTGAAGGGTTGGCTTCAAAGACATCTGGGACTGGCAATGAAAATAGGAATCCTATTCCATACAATAATGATAAGAGAGCTAAGCAAGGGAACGACCAGGATGAGATCAGCAACAATCCACGGAGCACAGTGTTGAATACAGAGCCTAGTGTTCTTGATGTTCATGTTATTGATGATAAGTCTAATGAAATGAATGCAGAGAAAGGTGATCGCAATATGCCAACTATGACTGGGTTGAATACTCAGTGCAAATTCCAGAGAGTTAGCAGATCTTCACCTTCTAGTATGCGGAGAAATTCAATGTCTGCAATTGATTATTATAAAATTGATAATGAAGTTGAGCGTCTTAGAGAAAGGTTAAGGACTGTGCAAGAGGGAAGAGAGAAGCTCAACTTCTCAGTGGGGCACAAAGAAAGGGAAAGGATTCAGCTGCAACTTTTGGAGGATATAGCAAGCCAGCTACAAGAGATACAGAAGTTAACAGAACCTAGAAAAGCTGAGGGCCAGGCCGCAAAGCTGCCTTCATTATCTAAGGTGTATTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

492

Amino Acids

56.07

Weight (kDa)

5.36

Isoelectric Point (pI)

53.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 80 - 168 1.1e-26 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1251
AarI CACCTGC 1 cut(s) 667
Acc36I ACCTGC 1 cut(s) 667
AccB7I CCANNNNNTGG 1 cut(s) 209
AccI GTMKAC 1 cut(s) 611
AccII CGCG 1 cut(s) 747
AciI CCGC 4 cut(s) 321, 520, 1219, 1440
AclWI GGATC 2 cut(s) 220, 580
AcoI YGGCCR 1 cut(s) 339
AcsI RAATTY 2 cut(s) 1181, 1225
AcuI CTGAAG 1 cut(s) 536
AcvI CACGTG 1 cut(s) 167
AfaI GTAC 3 cut(s) 237, 513, 880
AfiI CCNNNNNNNGG 2 cut(s) 209, 361
AflIII ACRYGT 2 cut(s) 166, 184
AhlI ACTAGT 1 cut(s) 725
AjnI CCWGG 3 cut(s) 507, 1026, 1434
Alw21I GWGCWC 1 cut(s) 1058
AlwI GGATC 2 cut(s) 220, 580
AlwNI CAGNNNCTG 1 cut(s) 514
AoxI GGCC 3 cut(s) 339, 1432, 1437
ApeKI GCWGC 9 cut(s) 25, 266, 275, 314, 323, 837, 914, 1360, 1447
ApoI RAATTY 2 cut(s) 1181, 1225
Asp700I GAANNNNTTC 1 cut(s) 1353
AspLEI GCGC 2 cut(s) 25, 354
AspS9I GGNCC 2 cut(s) 505, 1432
AsuHPI GGTGA 4 cut(s) 376, 837, 1148, 1197
AsuNHI GCTAGC 1 cut(s) 917
AvaII GGWCC 1 cut(s) 505
BaeGI GKGCMC 1 cut(s) 1338
BaeI ACNNNNGTAYC 2 cut(s) 1391, 1424
BalI TGGCCA 1 cut(s) 341
BauI CACGAG 1 cut(s) 270
BbrPI CACGTG 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 1058
BbvCI CCTCAGC 3 cut(s) 435, 888, 1428
BbvI GCAGC 9 cut(s) 12, 253, 262, 301, 310, 849, 901, 1347, 1434
BccI CCATC 3 cut(s) 113, 542, 598
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 3 cut(s) 509, 1028, 1436
BcuI ACTAGT 1 cut(s) 725
BfmI CTRYAG 2 cut(s) 135, 324
BfuAI ACCTGC 1 cut(s) 667
BglII AGATCT 2 cut(s) 31, 1199
BlpI GCTNAGC 1 cut(s) 1011
BmcAI AGTACT 2 cut(s) 237, 880
Bme1390I CCNGG 3 cut(s) 509, 1028, 1436
Bme18I GGWCC 1 cut(s) 505
BmgT120I GGNCC 2 cut(s) 505, 1432
BmrFI CCNGG 3 cut(s) 509, 1028, 1436
BmrI ACTGGG 1 cut(s) 1170
BmsI GCATC 7 cut(s) 10, 91, 132, 301, 380, 421, 703
BmtI GCTAGC 1 cut(s) 921
BmuI ACTGGG 1 cut(s) 1170
BplI GAGNNNNNCTC 2 cut(s) 1302, 1334
BpmI CTGGAG 1 cut(s) 794
Bpu10I CCTNAGC 3 cut(s) 435, 888, 1428
Bpu1102I GCTNAGC 1 cut(s) 1011
BpuEI CTTGAG 1 cut(s) 780
BsaAI YACGTR 1 cut(s) 167
BsaJI CCNNGG 2 cut(s) 855, 1049
BsaWI WCCGGW 1 cut(s) 662
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 361
Bse1I ACTGG 3 cut(s) 243, 967, 1165
Bse3DI GCAATG 2 cut(s) 638, 973
BseBI CCWGG 3 cut(s) 509, 1028, 1436
BseDI CCNNGG 2 cut(s) 855, 1049
BseGI GGATG 5 cut(s) 106, 411, 658, 694, 1036
BseLI CCNNNNNNNGG 2 cut(s) 209, 361
BseMI GCAATG 2 cut(s) 638, 973
BseMII CTCAG 6 cut(s) 426, 531, 902, 1187, 1341, 1419
BseNI ACTGG 3 cut(s) 243, 967, 1165
BseRI GAGGAG 2 cut(s) 311, 314
BseSI GKGCMC 1 cut(s) 1338
BseXI GCAGC 9 cut(s) 12, 253, 262, 301, 310, 849, 901, 1347, 1434
BsgI GTGCAG 1 cut(s) 46
Bsh1236I CGCG 1 cut(s) 747
BshFI GGCC 3 cut(s) 341, 1434, 1439
BsiHKAI GWGCWC 1 cut(s) 1058
BsiSI CCGG 2 cut(s) 152, 663
BslFI GGGAC 4 cut(s) 576, 837, 873, 973
BslI CCNNNNNNNGG 2 cut(s) 209, 361
BsmFI GGGAC 4 cut(s) 576, 837, 873, 973
BsmI GAATGC 1 cut(s) 1129
BsnI GGCC 3 cut(s) 341, 1434, 1439
Bsp1286I GDGCHC 2 cut(s) 1058, 1338
Bsp143I GATC 6 cut(s) 31, 212, 585, 1035, 1138, 1199
Bsp1720I GCTNAGC 1 cut(s) 1011
Bsp68I TCGCGA 1 cut(s) 747
BspACI CCGC 4 cut(s) 321, 520, 1219, 1440
BspANI GGCC 3 cut(s) 341, 1434, 1439
BspCNI CTCAG 6 cut(s) 427, 532, 901, 1186, 1340, 1420
BspFNI CGCG 1 cut(s) 747
BspMAI CTGCAG 1 cut(s) 328
BspMI ACCTGC 1 cut(s) 667
BspOI GCTAGC 1 cut(s) 921
BspPI GGATC 2 cut(s) 220, 580
BspQI GCTCTTC 1 cut(s) 252
BsrDI GCAATG 2 cut(s) 638, 973
BsrI ACTGG 3 cut(s) 243, 967, 1165
BssECI CCNNGG 2 cut(s) 855, 1049
BssMI GATC 6 cut(s) 31, 212, 585, 1035, 1138, 1199
BssNAI GTATAC 1 cut(s) 612
BssSI CACGAG 1 cut(s) 270
BssT1I CCWWGG 1 cut(s) 855
Bst1107I GTATAC 1 cut(s) 612
Bst2BI CACGAG 1 cut(s) 270
Bst2UI CCWGG 3 cut(s) 509, 1028, 1436
Bst4CI ACNGT 3 cut(s) 11, 1060, 1297
Bst6I CTCTTC 3 cut(s) 252, 470, 1303
BstBAI YACGTR 1 cut(s) 167
BstC8I GCNNGC 6 cut(s) 264, 356, 522, 919, 1384, 1388
BstDSI CCRYGG 1 cut(s) 1049
BstENI CCTNNNNNAGG 1 cut(s) 359
BstF5I GGATG 5 cut(s) 106, 411, 658, 694, 1036
BstFNI CGCG 1 cut(s) 747
BstHHI GCGC 2 cut(s) 25, 354
BstKTI GATC 6 cut(s) 34, 215, 588, 1038, 1141, 1202
BstMBI GATC 6 cut(s) 31, 212, 585, 1035, 1138, 1199
BstMWI GCNNNNNNNGC 5 cut(s) 272, 275, 320, 323, 431
BstNI CCWGG 3 cut(s) 509, 1028, 1436
BstNSI RCATGY 3 cut(s) 188, 266, 688
BstSCI CCNGG 3 cut(s) 507, 1026, 1434
BstSFI CTRYAG 2 cut(s) 135, 324
BstSLI GKGCMC 1 cut(s) 1338
BstUI CGCG 1 cut(s) 747
BstV1I GCAGC 9 cut(s) 12, 253, 262, 301, 310, 849, 901, 1347, 1434
BstX2I RGATCY 2 cut(s) 31, 1199
BstYI RGATCY 2 cut(s) 31, 1199
BstZ17I GTATAC 1 cut(s) 612
BsuRI GGCC 3 cut(s) 341, 1434, 1439
BtgI CCRYGG 1 cut(s) 1049
BtsCI GGATG 5 cut(s) 106, 411, 658, 694, 1036
BtsIMutI CAGTG 4 cut(s) 537, 1065, 1181, 1335
BtuMI TCGCGA 1 cut(s) 747
BveI ACCTGC 1 cut(s) 667
Cac8I GCNNGC 6 cut(s) 264, 356, 522, 919, 1384, 1388
CaiI CAGNNNCTG 1 cut(s) 514
CfoI GCGC 2 cut(s) 25, 354
Cfr13I GGNCC 2 cut(s) 505, 1432
CseI GACGC 1 cut(s) 1262
CsiI ACCWGGT 1 cut(s) 507
Csp6I GTAC 3 cut(s) 236, 512, 879
CviAII CATG 6 cut(s) 185, 263, 337, 343, 685, 1094
CviQI GTAC 3 cut(s) 236, 512, 879
DpnI GATC 6 cut(s) 33, 214, 587, 1037, 1140, 1201
DpnII GATC 6 cut(s) 31, 212, 585, 1035, 1138, 1199
EaeI YGGCCR 1 cut(s) 339
Eam1104I CTCTTC 3 cut(s) 252, 470, 1303
EarI CTCTTC 3 cut(s) 252, 470, 1303
Eco130I CCWWGG 1 cut(s) 855
Eco47I GGWCC 1 cut(s) 505
Eco57I CTGAAG 1 cut(s) 536
Eco72I CACGTG 1 cut(s) 167
EcoNI CCTNNNNNAGG 1 cut(s) 359
EcoRII CCWGG 3 cut(s) 507, 1026, 1434
EcoT14I CCWWGG 1 cut(s) 855
EcoT22I ATGCAT 1 cut(s) 690
ErhI CCWWGG 1 cut(s) 855
FaeI CATG 6 cut(s) 188, 266, 340, 346, 688, 1097
FalI AAGNNNNNCTT 2 cut(s) 1307, 1339
FaqI GGGAC 4 cut(s) 576, 837, 873, 973
FatI CATG 6 cut(s) 184, 262, 336, 342, 684, 1093
FauI CCCGC 1 cut(s) 513
FblI GTMKAC 1 cut(s) 611
FokI GGATG 5 cut(s) 113, 418, 665, 681, 1043
GlaI GCGC 2 cut(s) 24, 353
GsuI CTGGAG 1 cut(s) 794
HaeIII GGCC 3 cut(s) 341, 1434, 1439
HapII CCGG 2 cut(s) 152, 663
HgaI GACGC 1 cut(s) 1262
HhaI GCGC 2 cut(s) 25, 354
Hin1II CATG 6 cut(s) 188, 266, 340, 346, 688, 1097
Hin6I GCGC 2 cut(s) 23, 352
HinP1I GCGC 2 cut(s) 23, 352
HincII GTYRAC 1 cut(s) 1410
HindII GTYRAC 1 cut(s) 1410
HindIII AAGCTT 1 cut(s) 129
HinfI GANTC 5 cut(s) 41, 530, 659, 979, 1354
HpaI GTTAAC 1 cut(s) 1410
HpaII CCGG 2 cut(s) 152, 663
HphI GGTGA 4 cut(s) 376, 837, 1148, 1197
Hpy166II GTNNAC 3 cut(s) 612, 682, 1410
Hpy188I TCNGA 1 cut(s) 733
Hpy188III TCNNGA 6 cut(s) 287, 746, 755, 811, 1085, 1186
Hpy8I GTNNAC 3 cut(s) 612, 682, 1410
HpyAV CCTTC 5 cut(s) 361, 798, 929, 1218, 1461
HpyCH4III ACNGT 3 cut(s) 11, 1060, 1297
HpyCH4IV ACGT 1 cut(s) 166
HpyF10VI GCNNNNNNNGC 5 cut(s) 272, 275, 320, 323, 431
HpySE526I ACGT 1 cut(s) 166
Hsp92II CATG 6 cut(s) 188, 266, 340, 346, 688, 1097
HspAI GCGC 2 cut(s) 23, 352
KspAI GTTAAC 1 cut(s) 1410
Kzo9I GATC 6 cut(s) 31, 212, 585, 1035, 1138, 1199
LguI GCTCTTC 1 cut(s) 252
LmnI GCTCC 2 cut(s) 813, 1053
Lsp1109I GCAGC 9 cut(s) 12, 253, 262, 301, 310, 849, 901, 1347, 1434
LweI GCATC 7 cut(s) 10, 91, 132, 301, 380, 421, 703
MabI ACCWGGT 1 cut(s) 507
MaeII ACGT 1 cut(s) 166
MaeIII GTNAC 1 cut(s) 843
MalI GATC 6 cut(s) 33, 214, 587, 1037, 1140, 1201
MboI GATC 6 cut(s) 31, 212, 585, 1035, 1138, 1199
MboII GAAGA 7 cut(s) 29, 269, 487, 668, 783, 1194, 1320
MfeI CAATTG 2 cut(s) 375, 1239
MflI RGATCY 2 cut(s) 31, 1199
MhlI GDGCHC 2 cut(s) 1058, 1338
MlsI TGGCCA 1 cut(s) 341
MluNI TGGCCA 1 cut(s) 341
MlyI GAGTC 1 cut(s) 35
Mox20I TGGCCA 1 cut(s) 341
Mph1103I ATGCAT 1 cut(s) 690
MroXI GAANNNNTTC 1 cut(s) 1353
MscI TGGCCA 1 cut(s) 341
MseI TTAA 7 cut(s) 177, 453, 618, 722, 1289, 1409, 1474
MslI CAYNNNNRTG 3 cut(s) 95, 189, 341
Msp20I TGGCCA 1 cut(s) 341
MspA1I CMGCKG 2 cut(s) 323, 1360
MspI CCGG 2 cut(s) 152, 663
MspR9I CCNGG 3 cut(s) 509, 1028, 1436
MunI CAATTG 2 cut(s) 375, 1239
Mva1269I GAATGC 1 cut(s) 1129
MvaI CCWGG 3 cut(s) 509, 1028, 1436
MvnI CGCG 1 cut(s) 747
MwoI GCNNNNNNNGC 5 cut(s) 272, 275, 320, 323, 431
NdeII GATC 6 cut(s) 31, 212, 585, 1035, 1138, 1199
NheI GCTAGC 1 cut(s) 917
NlaIII CATG 6 cut(s) 188, 266, 340, 346, 688, 1097
NmuCI GTSAC 1 cut(s) 843
NruI TCGCGA 1 cut(s) 747
NsiI ATGCAT 1 cut(s) 690
NspI RCATGY 3 cut(s) 188, 266, 688
PaeI GCATGC 1 cut(s) 266
PaqCI CACCTGC 1 cut(s) 667
PciI ACATGT 1 cut(s) 184
PciSI GCTCTTC 1 cut(s) 252
PctI GAATGC 1 cut(s) 1129
PdmI GAANNNNTTC 1 cut(s) 1353
PfeI GAWTC 4 cut(s) 530, 659, 979, 1354
PflMI CCANNNNNTGG 1 cut(s) 209
PleI GAGTC 1 cut(s) 35
PmaCI CACGTG 1 cut(s) 167
PmlI CACGTG 1 cut(s) 167
PpsI GAGTC 1 cut(s) 35
Ppu21I YACGTR 1 cut(s) 167
PscI ACATGT 1 cut(s) 184
PsiI TTATAA 1 cut(s) 1251
Psp6I CCWGG 3 cut(s) 507, 1026, 1434
PspCI CACGTG 1 cut(s) 167
PspGI CCWGG 3 cut(s) 507, 1026, 1434
PspPI GGNCC 2 cut(s) 505, 1432
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 514
PsuI RGATCY 2 cut(s) 31, 1199
PvuII CAGCTG 1 cut(s) 1360
RruI TCGCGA 1 cut(s) 747
RsaI GTAC 3 cut(s) 237, 513, 880
RsaNI GTAC 3 cut(s) 236, 512, 879
RseI CAYNNNNRTG 3 cut(s) 95, 189, 341
SapI GCTCTTC 1 cut(s) 252
SaqAI TTAA 7 cut(s) 177, 453, 618, 722, 1289, 1409, 1474
Sau3AI GATC 6 cut(s) 31, 212, 585, 1035, 1138, 1199
Sau96I GGNCC 2 cut(s) 505, 1432
ScaI AGTACT 2 cut(s) 237, 880
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 3 cut(s) 509, 1028, 1436
SduI GDGCHC 2 cut(s) 1058, 1338
SexAI ACCWGGT 1 cut(s) 507
SfaNI GCATC 7 cut(s) 10, 91, 132, 301, 380, 421, 703
SfcI CTRYAG 2 cut(s) 135, 324
SinI GGWCC 1 cut(s) 505
SmiMI CAYNNNNRTG 3 cut(s) 95, 189, 341
SmlI CTYRAG 1 cut(s) 795
SmoI CTYRAG 1 cut(s) 795
SpeI ACTAGT 1 cut(s) 725
SphI GCATGC 1 cut(s) 266
SsiI CCGC 4 cut(s) 321, 520, 1219, 1440
StyD4I CCNGG 3 cut(s) 507, 1026, 1434
StyI CCWWGG 1 cut(s) 855
TaaI ACNGT 3 cut(s) 11, 1060, 1297
TaiI ACGT 1 cut(s) 169
TaqI TCGA 1 cut(s) 126
TatI WGTACW 2 cut(s) 235, 878
TauI GCSGC 1 cut(s) 1442
TfiI GAWTC 4 cut(s) 530, 659, 979, 1354
Tru1I TTAA 7 cut(s) 177, 453, 618, 722, 1289, 1409, 1474
Tru9I TTAA 7 cut(s) 177, 453, 618, 722, 1289, 1409, 1474
TscAI CASTG 4 cut(s) 544, 1065, 1181, 1335
TseFI GTSAC 1 cut(s) 843
TseI GCWGC 9 cut(s) 25, 266, 275, 314, 323, 837, 914, 1360, 1447
Tsp45I GTSAC 1 cut(s) 843
TspGWI ACGGA 2 cut(s) 860, 1066
TspRI CASTG 4 cut(s) 544, 1065, 1181, 1335
Van91I CCANNNNNTGG 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 505
XagI CCTNNNNNAGG 1 cut(s) 359
XapI RAATTY 2 cut(s) 1181, 1225
XceI RCATGY 3 cut(s) 188, 266, 688
XmiI GTMKAC 1 cut(s) 611
XmnI GAANNNNTTC 1 cut(s) 1353
ZrmI AGTACT 2 cut(s) 237, 880
Zsp2I ATGCAT 1 cut(s) 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.