RchiOBHm_Chr6g0272471

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006914
Physical Location & Seq
Reverse (-)
32799383 .. 32801690
2308 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1128 bp
ATGACAAAAGAAGCTTCAAAACTGGTGTATTTGTATCTCTCTCTGATATTTGCTTCTGTATCTTCAGCTTCCGCAAGCACTGTTAAATGTAGCTTCAAACAACATGGTAGTGGGAATACGATCCAAGGGATGTTTGTGTTCGGAAGCTCTCTGGTGGACAATGGCAACAATAACTTCCTGGAAACCGAGTCAAAAGCCGATTACTTGCCGTATGGAATCGACTTCCCAAATGGAACTACTGGGAGATTCACAAATGGTAAAAATGTTATTGATCTCCTCGGTGATCAGCTTAAGCTTCCTTTCATTCCACCATTTCAAGACCCCTCCACTAAAGGAAGTAAAATTGTTCATGGTGTCAACCATGCCTCTGGCTCTACTGGTATCTTAGATGATACAGGATTAGTTCTGGGTAATGCAACGAGCTTGAGTCAACAAGTTAAGGACTTCGAGGTTGTGACACTGGCAGAGTTAGAAGCAGAGCTAGGGTGCAATAGCTCTCAATCTCTACCAAGCTACTTGATTGTTGTAGGAGTTGGTGGGAATGATTACATGCTCAACTATTTCCTTAGGAAATTGTACCTCCAAATCAGTATTGAAACCTTCACTGCAAATCTCATCGACTCTCTAGCTCAGAAACTCCAGGAATTGTACATTTTGGGAGGTCGTAAATTCGTGTTAACGTCGCTAATGCCATTAGGCTACAGTCCTGTGATTAACAGGCCAAATCATATTGGCACCCCATCAGCTCTGAATTACGCAGCTCAGCTCTACAACGCTCACTTGAAGACATTGCTCGACTCTCTCAAAACTCACTTATCCGACTTCAATTTCGTTTTGGTTGACCAATATAACATTATATCTAATCTCACTTATTCTCCTCCTTCCACTACAGGCTTTAAAGACATACACAACCCTTGTTGTACAGTTTCCCTCTCTCAAGGTGGAATTGGAATATCATGCGATAGAGGCGGGAAGGCATGTGACGATAGGACGGCTTATATGTATTTTGATGGGTTACATCCAACAGAAGCTGTAAATGTTCAAATAGCAACCAAAGCATATGCTTCTACTCAACAGGACGAGGCTTACCCAATCAATGTCAGCCAACTTGCCCAGCTTCAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

375

Amino Acids

40.64

Weight (kDa)

5.58

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 45 - 350 5.2e-25 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 734
AciI CCGC 2 cut(s) 72, 969
AclWI GGATC 1 cut(s) 115
AcsI RAATTY 2 cut(s) 668, 1122
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 3 cut(s) 578, 650, 922
AflII CTTAAG 1 cut(s) 290
AgsI TTSAA 8 cut(s) 18, 97, 317, 596, 784, 826, 1043, 1121
AjnI CCWGG 2 cut(s) 177, 639
AlwI GGATC 1 cut(s) 115
AlwNI CAGNNNCTG 1 cut(s) 1031
AoxI GGCC 1 cut(s) 719
ApeKI GCWGC 1 cut(s) 758
ApoI RAATTY 2 cut(s) 668, 1122
ArsI GACNNNNNNTTYG 2 cut(s) 812, 844
AsuHPI GGTGA 1 cut(s) 293
AxyI CCTNAGG 1 cut(s) 566
BanI GGYRCC 1 cut(s) 734
BbsI GAAGAC 1 cut(s) 791
BbvI GCAGC 1 cut(s) 770
BccI CCATC 2 cut(s) 748, 1004
BceAI ACGGC 2 cut(s) 193, 1008
BciT130I CCWGG 2 cut(s) 179, 641
BclI TGATCA 1 cut(s) 283
BfaI CTAG 2 cut(s) 482, 626
BfmI CTRYAG 2 cut(s) 700, 888
BfrI CTTAAG 1 cut(s) 290
BisI GCNGC 1 cut(s) 759
BlpI GCTNAGC 1 cut(s) 762
BlsI GCNGC 1 cut(s) 760
Bme1390I CCNGG 2 cut(s) 179, 641
BmiI GGNNCC 1 cut(s) 736
BmrFI CCNGG 2 cut(s) 179, 641
BmrI ACTGGG 1 cut(s) 249
BmuI ACTGGG 1 cut(s) 249
BpiI GAAGAC 1 cut(s) 791
BpmI CTGGAG 1 cut(s) 623
Bpu1102I GCTNAGC 1 cut(s) 762
BpuEI CTTGAG 2 cut(s) 445, 921
BsaJI CCNNGG 2 cut(s) 124, 277
BsaXI ACNNNNNCTCC 2 cut(s) 859, 889
Bse1I ACTGG 4 cut(s) 27, 244, 382, 465
Bse21I CCTNAGG 1 cut(s) 566
Bse3DI GCAATG 1 cut(s) 788
BseBI CCWGG 2 cut(s) 179, 641
BseDI CCNNGG 2 cut(s) 124, 277
BseGI GGATG 2 cut(s) 135, 1018
BseMI GCAATG 1 cut(s) 788
BseMII CTCAG 2 cut(s) 644, 776
BseNI ACTGG 4 cut(s) 27, 244, 382, 465
BseRI GAGGAG 2 cut(s) 266, 867
BseXI GCAGC 1 cut(s) 770
BseYI CCCAGC 1 cut(s) 1113
BshFI GGCC 1 cut(s) 721
BshNI GGYRCC 1 cut(s) 734
BsnI GGCC 1 cut(s) 721
Bsp1407I TGTACA 2 cut(s) 648, 920
Bsp143I GATC 3 cut(s) 120, 271, 283
Bsp1720I GCTNAGC 1 cut(s) 762
BspACI CCGC 2 cut(s) 72, 969
BspANI GGCC 1 cut(s) 721
BspCNI CTCAG 2 cut(s) 643, 775
BspLI GGNNCC 1 cut(s) 736
BspPI GGATC 1 cut(s) 115
BspT107I GGYRCC 1 cut(s) 734
BspTI CTTAAG 1 cut(s) 290
BsrDI GCAATG 1 cut(s) 788
BsrGI TGTACA 2 cut(s) 648, 920
BsrI ACTGG 4 cut(s) 27, 244, 382, 465
BssECI CCNNGG 2 cut(s) 124, 277
BssMI GATC 3 cut(s) 120, 271, 283
BssT1I CCWWGG 1 cut(s) 124
Bst2UI CCWGG 2 cut(s) 179, 641
Bst4CI ACNGT 3 cut(s) 82, 704, 925
BstAFI CTTAAG 1 cut(s) 290
BstAUI TGTACA 2 cut(s) 648, 920
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 4 cut(s) 385, 566, 630, 762
BstF5I GGATG 2 cut(s) 135, 1018
BstKTI GATC 3 cut(s) 123, 274, 286
BstMBI GATC 3 cut(s) 120, 271, 283
BstMWI GCNNNNNNNGC 2 cut(s) 966, 1055
BstNI CCWGG 2 cut(s) 179, 641
BstNSI RCATGY 2 cut(s) 553, 981
BstSCI CCNGG 2 cut(s) 177, 639
BstSFI CTRYAG 2 cut(s) 700, 888
BstV1I GCAGC 1 cut(s) 770
BstV2I GAAGAC 1 cut(s) 791
BstXI CCANNNNNNTGG 1 cut(s) 368
Bsu36I CCTNAGG 1 cut(s) 566
BsuRI GGCC 1 cut(s) 721
BtsCI GGATG 2 cut(s) 135, 1018
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 3 cut(s) 78, 458, 603
Cac8I GCNNGC 1 cut(s) 76
CaiI CAGNNNCTG 1 cut(s) 1031
Csp6I GTAC 3 cut(s) 577, 649, 921
CviAII CATG 6 cut(s) 104, 350, 362, 550, 957, 978
CviQI GTAC 3 cut(s) 577, 649, 921
DdeI CTNAG 4 cut(s) 385, 566, 630, 762
DpnI GATC 3 cut(s) 122, 273, 285
DpnII GATC 3 cut(s) 120, 271, 283
DraI TTTAAA 1 cut(s) 898
Eco130I CCWWGG 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 48
Eco81I CCTNAGG 1 cut(s) 566
EcoRII CCWGG 2 cut(s) 177, 639
EcoT14I CCWWGG 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 124
FaeI CATG 6 cut(s) 107, 353, 365, 553, 960, 981
FatI CATG 6 cut(s) 103, 349, 361, 549, 956, 977
FauI CCCGC 1 cut(s) 962
FauNDI CATATG 1 cut(s) 1060
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 1 cut(s) 759
FokI GGATG 2 cut(s) 142, 1005
Fsp4HI GCNGC 1 cut(s) 759
FspBI CTAG 2 cut(s) 482, 626
GluI GCNGC 1 cut(s) 759
GsaI CCCAGC 1 cut(s) 1117
GsuI CTGGAG 1 cut(s) 623
HaeIII GGCC 1 cut(s) 721
Hin1II CATG 6 cut(s) 107, 353, 365, 553, 960, 981
HincII GTYRAC 4 cut(s) 358, 431, 678, 841
HindII GTYRAC 4 cut(s) 358, 431, 678, 841
HindIII AAGCTT 2 cut(s) 12, 293
HinfI GANTC 6 cut(s) 188, 216, 246, 427, 620, 797
HpaI GTTAAC 1 cut(s) 678
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 5 cut(s) 157, 358, 431, 678, 841
Hpy188I TCNGA 5 cut(s) 45, 143, 633, 750, 820
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 5 cut(s) 157, 358, 431, 678, 841
Hpy99I CGWCG 1 cut(s) 685
HpyAV CCTTC 3 cut(s) 610, 891, 967
HpyCH4III ACNGT 3 cut(s) 82, 704, 925
HpyCH4IV ACGT 1 cut(s) 680
HpyCH4V TGCA 3 cut(s) 416, 489, 608
HpyF10VI GCNNNNNNNGC 2 cut(s) 966, 1055
HpyF3I CTNAG 4 cut(s) 385, 566, 630, 762
HpySE526I ACGT 1 cut(s) 680
Hsp92II CATG 6 cut(s) 107, 353, 365, 553, 960, 981
Ksp22I TGATCA 1 cut(s) 283
KspAI GTTAAC 1 cut(s) 678
Kzo9I GATC 3 cut(s) 120, 271, 283
Lsp1109I GCAGC 1 cut(s) 770
MaeI CTAG 2 cut(s) 482, 626
MaeII ACGT 1 cut(s) 680
MaeIII GTNAC 3 cut(s) 454, 980, 1014
MalI GATC 3 cut(s) 122, 273, 285
MboI GATC 3 cut(s) 120, 271, 283
MboII GAAGA 2 cut(s) 54, 796
MluCI AATT 8 cut(s) 342, 572, 644, 668, 751, 826, 945, 1122
MlyI GAGTC 4 cut(s) 197, 436, 614, 791
MmeI TCCRAC 2 cut(s) 843, 1046
MseI TTAA 7 cut(s) 84, 291, 438, 677, 714, 897, 1126
MslI CAYNNNNRTG 1 cut(s) 108
MspCI CTTAAG 1 cut(s) 290
MspR9I CCNGG 2 cut(s) 179, 641
MvaI CCWGG 2 cut(s) 179, 641
MwoI GCNNNNNNNGC 2 cut(s) 966, 1055
NdeI CATATG 1 cut(s) 1060
NdeII GATC 3 cut(s) 120, 271, 283
NlaIII CATG 6 cut(s) 107, 353, 365, 553, 960, 981
NlaIV GGNNCC 1 cut(s) 736
NmuCI GTSAC 2 cut(s) 454, 980
NspI RCATGY 2 cut(s) 553, 981
PfeI GAWTC 2 cut(s) 216, 246
PfoI TCCNGGA 2 cut(s) 177, 639
PkrI GCNGC 1 cut(s) 760
PleI GAGTC 4 cut(s) 196, 435, 614, 791
PpsI GAGTC 4 cut(s) 196, 435, 614, 791
Psp6I CCWGG 2 cut(s) 177, 639
PspFI CCCAGC 1 cut(s) 1113
PspGI CCWGG 2 cut(s) 177, 639
PspN4I GGNNCC 1 cut(s) 736
PstNI CAGNNNCTG 1 cut(s) 1031
RsaI GTAC 3 cut(s) 578, 650, 922
RsaNI GTAC 3 cut(s) 577, 649, 921
RseI CAYNNNNRTG 1 cut(s) 108
SaqAI TTAA 7 cut(s) 84, 291, 438, 677, 714, 897, 1126
SatI GCNGC 1 cut(s) 759
Sau3AI GATC 3 cut(s) 120, 271, 283
SchI GAGTC 4 cut(s) 197, 436, 614, 791
ScrFI CCNGG 2 cut(s) 179, 641
SfcI CTRYAG 2 cut(s) 700, 888
SmiMI CAYNNNNRTG 1 cut(s) 108
SmlI CTYRAG 3 cut(s) 290, 424, 936
SmoI CTYRAG 3 cut(s) 290, 424, 936
Sse9I AATT 8 cut(s) 342, 572, 644, 668, 751, 826, 945, 1122
SsiI CCGC 2 cut(s) 72, 969
SspMI CTAG 2 cut(s) 482, 626
StyD4I CCNGG 2 cut(s) 177, 639
StyI CCWWGG 1 cut(s) 124
TaaI ACNGT 3 cut(s) 82, 704, 925
TaiI ACGT 1 cut(s) 683
TaqI TCGA 4 cut(s) 219, 447, 618, 795
TasI AATT 8 cut(s) 342, 572, 644, 668, 751, 826, 945, 1122
TatI WGTACW 2 cut(s) 648, 920
TfiI GAWTC 2 cut(s) 216, 246
Tru1I TTAA 7 cut(s) 84, 291, 438, 677, 714, 897, 1126
Tru9I TTAA 7 cut(s) 84, 291, 438, 677, 714, 897, 1126
TscAI CASTG 3 cut(s) 85, 465, 610
TseFI GTSAC 2 cut(s) 454, 980
TseI GCWGC 1 cut(s) 758
Tsp45I GTSAC 2 cut(s) 454, 980
TspDTI ATGAA 2 cut(s) 292, 338
TspRI CASTG 3 cut(s) 85, 465, 610
Vha464I CTTAAG 1 cut(s) 290
XapI RAATTY 2 cut(s) 668, 1122
XceI RCATGY 2 cut(s) 553, 981
XspI CTAG 2 cut(s) 482, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.