RchiOBHm_Chr5g0075701

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012601
Physical Location & Seq
Forward (+)
81496072 .. 81496875
804 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 804 bp
ATGACAACCAAGAATGGGAACAGAAGATGGGCAGTTTTTGTATGTTGTGGTTTTGGGTATGTGTCAGCAACTGATGACTACAAATTTGTTATTCTATTCCATGATTTACCTTTTTTGTTGGGTGTTCACATTTTCTCGGTGAGAGATAATTCATGGAAAATTGTTCAAGTCGCAGGCATTCACGACGACTGGATCTTCAGCAGCAAGGGGGTTCTTTCAAATGAAGTACTCCATTGGATCACTGAGAACAAAACTAATGAGCTAGTTATCTCTGCTTTCGATTTGGCTGAGGAGGAGTTCCATAAAGAGTCGTTGCTATTCCCTGCGGATGTAGACTGCAACATTTATGATCTAGACTACAACATTTGTAATCCGTGGAAGCGGATAGAACTGGGGTGTTTAGATGGATGCCTTTGCATACACTCTCTGAATACTAAAGCTGGTGGGGATATTGAAGTCTGGGTGATGAGAGAATACAGAGTACTTGAATCTTGGATCAAACTCTTTAAATTTACTGTAGACGATATTCCAGGTGTGTTTGTTTCACAAATTCCTTTGTTTCAATTTTTCGTTTCGGAAAGTGGTACTGCTTTCATCAATTCAGATTTCAAGGAGTTGGTCAGAATTGAATGCCACAAAGAAGAGAAGCCGGCTTTGAGTGGCCGGTATAAGATTGAGAAGTGGGAGCCCTTGAATTTAGGTTTATGGGAGCAGATTCTTGAAAGTTTTCAGAAGGAAAAGGAGGATGCAAAATTTTTTTCGGTGATGTTTTACGATGAAACTTTAGTTTCAGTTCCTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1

Protein Analysis

267

Amino Acids

31.01

Weight (kDa)

4.86

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 14 - 210 4e-13 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 333, 519
AciI CCGC 2 cut(s) 326, 382
AclWI GGATC 3 cut(s) 200, 245, 503
AcoI YGGCCR 1 cut(s) 661
AcsI RAATTY 5 cut(s) 83, 509, 549, 694, 752
AcuI CTGAAG 1 cut(s) 181
AfaI GTAC 3 cut(s) 228, 483, 586
AfiI CCNNNNNNNGG 1 cut(s) 15
AgsI TTSAA 9 cut(s) 167, 219, 455, 488, 563, 610, 629, 694, 722
AjnI CCWGG 1 cut(s) 529
AluBI AGCT 2 cut(s) 262, 440
AluI AGCT 2 cut(s) 262, 440
AlwI GGATC 3 cut(s) 200, 245, 503
AlwNI CAGNNNCTG 2 cut(s) 71, 797
AoxI GGCC 1 cut(s) 661
ApeKI GCWGC 1 cut(s) 201
ApoI RAATTY 5 cut(s) 83, 509, 549, 694, 752
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 1 cut(s) 726
AsuHPI GGTGA 3 cut(s) 151, 475, 775
BanII GRGCYC 1 cut(s) 690
BbvCI CCTCAGC 1 cut(s) 288
BbvI GCAGC 1 cut(s) 213
BccI CCATC 2 cut(s) 21, 398
BciT130I CCWGG 1 cut(s) 531
BfaI CTAG 2 cut(s) 263, 353
BfmI CTRYAG 1 cut(s) 516
BisI GCNGC 1 cut(s) 202
BlsI GCNGC 1 cut(s) 203
BmcAI AGTACT 2 cut(s) 228, 483
Bme1390I CCNGG 1 cut(s) 531
BmiI GGNNCC 1 cut(s) 687
BmrFI CCNGG 1 cut(s) 531
BmrI ACTGGG 1 cut(s) 401
BmsI GCATC 2 cut(s) 398, 736
BmuI ACTGGG 1 cut(s) 401
Bpu10I CCTNAGC 1 cut(s) 288
BsaJI CCNNGG 1 cut(s) 374
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse118I RCCGGY 2 cut(s) 649, 663
Bse1I ACTGG 2 cut(s) 194, 396
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 1 cut(s) 374
BseGI GGATG 3 cut(s) 334, 413, 751
BseLI CCNNNNNNNGG 1 cut(s) 15
BseMII CTCAG 2 cut(s) 234, 279
BseNI ACTGG 2 cut(s) 194, 396
BseRI GAGGAG 2 cut(s) 305, 308
BseXI GCAGC 1 cut(s) 213
BshFI GGCC 1 cut(s) 663
BsiSI CCGG 2 cut(s) 650, 664
BslI CCNNNNNNNGG 1 cut(s) 15
BsmI GAATGC 2 cut(s) 177, 635
BsnI GGCC 1 cut(s) 663
Bsp1286I GDGCHC 1 cut(s) 690
Bsp143I GATC 4 cut(s) 192, 237, 349, 495
BspACI CCGC 2 cut(s) 326, 382
BspANI GGCC 1 cut(s) 663
BspCNI CTCAG 2 cut(s) 235, 280
BspLI GGNNCC 1 cut(s) 687
BspPI GGATC 3 cut(s) 200, 245, 503
BsrFI RCCGGY 2 cut(s) 649, 663
BsrI ACTGG 2 cut(s) 194, 396
BssAI RCCGGY 2 cut(s) 649, 663
BssECI CCNNGG 1 cut(s) 374
BssMI GATC 4 cut(s) 192, 237, 349, 495
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 1 cut(s) 517
Bst6I CTCTTC 1 cut(s) 636
BstC8I GCNNGC 2 cut(s) 175, 651
BstDEI CTNAG 2 cut(s) 243, 288
BstDSI CCRYGG 1 cut(s) 374
BstF5I GGATG 3 cut(s) 334, 413, 751
BstKTI GATC 4 cut(s) 195, 240, 352, 498
BstMBI GATC 4 cut(s) 192, 237, 349, 495
BstNI CCWGG 1 cut(s) 531
BstSCI CCNGG 1 cut(s) 529
BstSFI CTRYAG 1 cut(s) 516
BstV1I GCAGC 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 192
BstYI RGATCY 1 cut(s) 192
BsuRI GGCC 1 cut(s) 663
BtgI CCRYGG 1 cut(s) 374
BtsCI GGATG 3 cut(s) 334, 413, 751
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 2 cut(s) 175, 651
CaiI CAGNNNCTG 2 cut(s) 71, 797
Cfr10I RCCGGY 2 cut(s) 649, 663
Csp6I GTAC 3 cut(s) 227, 482, 585
CviAII CATG 2 cut(s) 101, 153
CviJI RGCY 7 cut(s) 262, 287, 440, 649, 653, 663, 688
CviKI_1 RGCY 7 cut(s) 262, 287, 440, 649, 653, 663, 688
CviQI GTAC 3 cut(s) 227, 482, 585
DdeI CTNAG 2 cut(s) 243, 288
DpnI GATC 4 cut(s) 194, 239, 351, 497
DpnII GATC 4 cut(s) 192, 237, 349, 495
DraI TTTAAA 1 cut(s) 508
EaeI YGGCCR 1 cut(s) 661
Eam1104I CTCTTC 1 cut(s) 636
EarI CTCTTC 1 cut(s) 636
Eco24I GRGCYC 1 cut(s) 690
Eco57I CTGAAG 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 529
EcoT38I GRGCYC 1 cut(s) 690
FaeI CATG 2 cut(s) 104, 156
FaiI YATR 9 cut(s) 43, 60, 102, 154, 303, 348, 419, 669, 706
FatI CATG 2 cut(s) 100, 152
FblI GTMKAC 2 cut(s) 333, 519
Fnu4HI GCNGC 1 cut(s) 202
FokI GGATG 3 cut(s) 341, 420, 758
FriOI GRGCYC 1 cut(s) 690
Fsp4HI GCNGC 1 cut(s) 202
FspBI CTAG 2 cut(s) 263, 353
GluI GCNGC 1 cut(s) 202
HaeIII GGCC 1 cut(s) 663
HapII CCGG 2 cut(s) 650, 664
Hin1II CATG 2 cut(s) 104, 156
HinfI GANTC 3 cut(s) 308, 488, 715
HpaII CCGG 2 cut(s) 650, 664
HphI GGTGA 3 cut(s) 151, 475, 775
Hpy166II GTNNAC 3 cut(s) 127, 334, 520
Hpy188I TCNGA 5 cut(s) 429, 577, 604, 623, 732
Hpy188III TCNNGA 4 cut(s) 182, 353, 719, 797
Hpy8I GTNNAC 3 cut(s) 127, 334, 520
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 1 cut(s) 727
HpyCH4III ACNGT 1 cut(s) 517
HpyCH4V TGCA 3 cut(s) 339, 417, 749
HpyF3I CTNAG 2 cut(s) 243, 288
Hsp92II CATG 2 cut(s) 104, 156
KroI GCCGGC 1 cut(s) 649
KroNI GCCGGC 1 cut(s) 651
Kzo9I GATC 4 cut(s) 192, 237, 349, 495
LmnI GCTCC 2 cut(s) 685, 709
Lsp1109I GCAGC 1 cut(s) 213
LweI GCATC 2 cut(s) 398, 736
MaeI CTAG 2 cut(s) 263, 353
MalI GATC 4 cut(s) 194, 239, 351, 497
MboI GATC 4 cut(s) 192, 237, 349, 495
MboII GAAGA 3 cut(s) 36, 187, 653
MflI RGATCY 1 cut(s) 192
MhlI GDGCHC 1 cut(s) 690
MlyI GAGTC 1 cut(s) 317
MnlI CCTC 3 cut(s) 283, 286, 736
MroNI GCCGGC 1 cut(s) 649
MroXI GAANNNNTTC 1 cut(s) 726
MseI TTAA 1 cut(s) 507
MspI CCGG 2 cut(s) 650, 664
MspR9I CCNGG 1 cut(s) 531
Mva1269I GAATGC 2 cut(s) 177, 635
MvaI CCWGG 1 cut(s) 531
NaeI GCCGGC 1 cut(s) 651
NdeII GATC 4 cut(s) 192, 237, 349, 495
NgoMIV GCCGGC 1 cut(s) 649
NlaIII CATG 2 cut(s) 104, 156
NlaIV GGNNCC 1 cut(s) 687
PctI GAATGC 2 cut(s) 177, 635
PdiI GCCGGC 1 cut(s) 651
PdmI GAANNNNTTC 1 cut(s) 726
PfeI GAWTC 2 cut(s) 488, 715
PkrI GCNGC 1 cut(s) 203
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 529
PspN4I GGNNCC 1 cut(s) 687
PstNI CAGNNNCTG 2 cut(s) 71, 797
PsuI RGATCY 1 cut(s) 192
RsaI GTAC 3 cut(s) 228, 483, 586
RsaNI GTAC 3 cut(s) 227, 482, 585
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 1 cut(s) 202
Sau3AI GATC 4 cut(s) 192, 237, 349, 495
ScaI AGTACT 2 cut(s) 228, 483
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 531
SduI GDGCHC 1 cut(s) 690
SetI ASST 5 cut(s) 112, 264, 442, 535, 703
SfaNI GCATC 2 cut(s) 398, 736
SfcI CTRYAG 1 cut(s) 516
SsiI CCGC 2 cut(s) 326, 382
SspMI CTAG 2 cut(s) 263, 353
StyD4I CCNGG 1 cut(s) 529
TaaI ACNGT 1 cut(s) 517
TaqI TCGA 1 cut(s) 279
TatI WGTACW 2 cut(s) 226, 481
TfiI GAWTC 2 cut(s) 488, 715
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TscAI CASTG 1 cut(s) 247
TseI GCWGC 1 cut(s) 201
TspDTI ATGAA 4 cut(s) 141, 237, 583, 792
TspGWI ACGGA 1 cut(s) 363
TspRI CASTG 1 cut(s) 247
XapI RAATTY 5 cut(s) 83, 509, 549, 694, 752
XbaI TCTAGA 1 cut(s) 352
XmiI GTMKAC 2 cut(s) 333, 519
XmnI GAANNNNTTC 1 cut(s) 726
XspI CTAG 2 cut(s) 263, 353
ZrmI AGTACT 2 cut(s) 228, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.