Rorug04G0064600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000336
Physical Location & Seq
Forward (+)
10039656 .. 10048131
8476 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0064600.1

Sequence Viewer

Length: 2049 bp
ATGCCCATCTCTCTTTCTACTCAAACCCACTTCTTTCCTTTGACCATGGGCTCTCTCCTCCCAATTCTTGACCTCAATGCCTCCCCTCAATCCGCCCCCAATTCAACCCTAGCCACCTCCACCATATCCCTCAAGGTCCCCAAAATCGAACCCAAGCCCGAACCCTGCGACGACCCAGCTCCCCAACCCCCCTTCGATCCCAGCCCCAGCTCCGACATTTTCCCCAATCCCCAAACTACCCCCCTCCCCCACCAGACCTCGATCGTCGCCGCCGCGCCGGACCACGTCTACTCCGAGTTCCACCGCATTTCGGAGCTCTTCCACACCGCGTTCGCGAAAGGGATTCAGAACTGCGACGGCGGCGGCGGAGACGAGGACGGCGTCGTTCTGGACCCGGAGTCCGGCGCGATTGTTCCCGTGGAGGACAACAACCAGCAGCAGCAGCTGGCGGAGGTGGTGCAGCGGAAGAAGTACCCGCAGCGGTCGAACGAGCTCGTCCGGGTCACCGATCTCAGCGACGAGGACCACCGCTACTTCCGTGAGGTCGTCAGGAAGACCCGGATGCTCTACGATTCGATTCGGATCCTCTCGGTGGCGGAGGAGGACAAGAAGAATCCGGGTCAGGGGAAACGGACCCGGGGCGACTTGCGGGCCGCCTCCGTGCTCCGGGACCGTGGGCTCTGGCTCAACCGTGATAAGCGGATTGTGGGGTCCATTCCGGGAGTCTATGTTGGTGACCTCTTCTTCTTTCGCATGGAGCTATGTGTGGTTGGGATACATGGACAAGTCCAAGCTGGAATTGACTATCTTCCGGGGAGCCAGAGCTCGAACAGGGAGCCGATTGCGACGAGCATTATCGTCTCCGGCGGGTACGAGGACGATGAGGATGCTGGGGATATTATTATCTACACAGGACACGGAGGGCAGGACAAGTTCAACAGGCAATGTGCGCACCAGAAGCTGGAGGGTGGGAACTTGGCGTTGGAGAGGAGTATGCATTATGGGATTGAAGTGAGGGTTATTAGGGGGAGGAAGATTCATGGAGCTGTTTCGAGTAAGGTGTATGTTTATGATGGGTTGTATAGGATTCTTGAAACTTGGCTTGATGTTGGGAAGTCGGGGTTTGGTGTTTATAAGTTTAAGCTTTTGAGAATCGAGGGGCAGCCTGAGATGGGGAGCTCCATTCTTAAGTTTGCCGAGAGTCTTAGGAGTAGGCCGTTGAGTGTGAGGCCGAGGGGTTATCTTAGTTTGGATCTTTCGCAGCAGAGGGAGAAGATTCCGGTGTTGCTTTTCAATGACATTGATGCTGATCAGGACCCCTTGTATTATGAGTATCTTAGGACACCTGCATTTCCAACTCATGTGTATCATCAGTCGGCAAATGGTACTGGCTGTGAATGTGTTAACGGCTGCAATGAAAATTGCTTTTGTGCTATGAAAAATGGAGGGGAGTTTCCTTATGATCAGAATGGGTTTCTTTTGAGGGGGAAGCCTGTTGTATTTGAGTGTGGGACCTTCTGCCGTTGCCCCCCGCAGTGTCGGAATCGTGTCTCCCAACATGGATTGAGAAATAGACTGGAAGTGTTTAGATCAAGGGAAACAGGTTGGGGCGTTAGGTCTTTGGACTTGATACATGCCGGCGCTTTTATATGTGAGTATACAGGAGTTATTCTCACGAGGGAGCAGGCTCAAATTTTTTCCATGGATGGTGATAGCTTGATCTATCCCCATCGGTTTTCTGATAAATGGGCAGAATGGGGAGATTTATCTCAGATCTATCCTGATTATGCGCGTCCAACTTATCCGGCAATCCCACCGCTGGATTTTGCAATGGATGTGTCAAAAATGAGGAATGTTGCTTGTTATATGAGCCAGAGTACCACTCCAAATGTGATGGTGCAGTTCGTTCTCTATGATCACAACAACTTGATGTTTCCTCATCTTATGCTATTTGCAATGGAGAACATTCCTCCTCTGAGGGAGTTAAGCCTTGACTATGGGGTGGCGGTGGCTGATGAATGGACAGGGAAACTTGCTATCTGTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

682

Amino Acids

76.45

Weight (kDa)

5.81

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 236 - 388 3.7e-52 SAD/SRA domain
Pre-SET PF05033 420 - 516 2.5e-20 Pre-SET motif
SET PF00856 535 - 667 3.9e-11 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000336)

Species Orthologous Gene IDs
malus_domestica MD05G1024100.v1.1
rosa_chinensis RchiOBHm_Chr2g0152291 RchiOBHm_Chr4g0387411
rosa_laevigata RLG00000009698 RLG00000009699 RLG00000009700 RLG00000023615 RLG00000024265 RLG00000034445 RLG00000036567
rosa_multiflora Rmu_co8268067.1_g000001 Rmu_co8313555.1_g000001 Rmu_co8324697.1_g000001 Rmu_sc0000318.1_g000023 Rmu_sc0000342.1_g000016 Rmu_sc0000447.1_g000010 Rmu_sc0000528.1_g000009 Rmu_sc0000539.1_g000060 Rmu_sc0000929.1_g000023 Rmu_sc0001493.1_g000081 Rmu_sc0001674.1_g000003 Rmu_sc0001791.1_g000023 Rmu_sc0001822.1_g000005 Rmu_sc0001822.1_g000033 Rmu_sc0002061.1_g000010 Rmu_sc0002286.1_g000012 Rmu_sc0002979.1_g000009 Rmu_sc0003158.1_g000016 Rmu_sc0003221.1_g000001 Rmu_sc0003641.1_g000029 Rmu_sc0003742.1_g000012 Rmu_sc0003814.1_g000004 Rmu_sc0003823.1_g000005 Rmu_sc0004002.1_g000005 Rmu_sc0004564.1_g000005 Rmu_sc0005415.1_g000003 Rmu_sc0006050.1_g000025 Rmu_sc0006555.1_g000002 Rmu_sc0009954.1_g000020 Rmu_sc0010421.1_g000007 Rmu_sc0010607.1_g000002 Rmu_sc0011044.1_g000005 Rmu_sc0011497.1_g000015 Rmu_sc0014532.1_g000004 Rmu_sc0014745.1_g000005 Rmu_sc0014821.1_g000001 Rmu_sc0015038.1_g000002 Rmu_sc0015454.1_g000004 Rmu_sc0019663.1_g000002 Rmu_sc0027475.1_g000003 Rmu_sc0028826.1_g000002 Rmu_ssc0000117.1_g000019 Rmu_ssc0000175.1_g000034
rosa_roxburghii Rroxscaffold_3G00237080 Rroxscaffold_4G00288290 Rroxscaffold_6G00397510
rosa_rugosa Rorug01G0035000 Rorug01G0190900 Rorug01G0208500 Rorug01G0208600 Rorug01G0208600 Rorug01G0344900 Rorug01G0479500 Rorug01G0479600 Rorug02G0230400 Rorug02G0320900 Rorug02G0433300 Rorug03G0147400 Rorug03G0169700.1 Rorug03G0169800 Rorug03G0198300 Rorug04G0022900 Rorug04G0064600 Rorug04G0322100 Rorug04G0388900 Rorug05G0153200 Rorug05G0231600 Rorug05G0231700 Rorug05G0231800 Rorug05G0276200 Rorug05G0292400 Rorug05G0314700 Rorug06G0092000 Rorug06G0260900 Rorug06G0273600 RorugPtG0003200
rosa_samantha Rh1AG264100 Rh1AG292500 Rh1AG423800 Rh2AG098000 Rh2AG232700 Rh2AG361100 Rh2AG372900 Rh2BG099200 Rh2BG246000 Rh2BG260600 Rh2BG378400 Rh3BG142300 Rh3BG252900 Rh3BG267700 Rh3DG302400 Rh5BG059800 Rh5BG320000 Rh5BG530500 Rh6AG089300 Rh6AG089400 Rh6AG127400 Rh6AG376700 Rh7CG488700 Rh7DG265900
rosa_wichuraiana Rw2G040640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1134
AarI CACCTGC 1 cut(s) 1354
Acc16I TGCGCA 1 cut(s) 951
Acc36I ACCTGC 1 cut(s) 1354
AccB7I CCANNNNNTGG 1 cut(s) 961
AccI GTMKAC 2 cut(s) 288, 1658
AccII CGCG 5 cut(s) 275, 329, 335, 407, 1792
AclWI GGATC 4 cut(s) 191, 577, 590, 1260
AcsI RAATTY 1 cut(s) 1692
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 4 cut(s) 473, 872, 1387, 1879
AfiI CCNNNNNNNGG 8 cut(s) 310, 401, 592, 623, 666, 961, 1172, 1819
AflII CTTAAG 1 cut(s) 1187
AgsI TTSAA 5 cut(s) 105, 937, 1010, 1094, 1294
AhdI GACNNNNNGTC 1 cut(s) 397
AjiI CACGTC 1 cut(s) 286
AjuI GAANNNNNNNTTGG 2 cut(s) 965, 997
Alw21I GWGCWC 5 cut(s) 318, 495, 666, 827, 1181
Alw26I GTCTC 3 cut(s) 363, 865, 1555
AlwI GGATC 4 cut(s) 191, 577, 590, 1260
AlwNI CAGNNNCTG 2 cut(s) 445, 961
Ama87I CYCGRG 1 cut(s) 636
AoxI GGCC 3 cut(s) 651, 1214, 1229
ApeKI GCWGC 8 cut(s) 436, 439, 442, 460, 478, 1162, 1261, 1410
ApoI RAATTY 1 cut(s) 1692
AspLEI GCGC 5 cut(s) 277, 407, 952, 1643, 1792
AsuC2I CCSGG 9 cut(s) 395, 500, 559, 618, 637, 638, 668, 720, 813
AsuHPI GGTGA 3 cut(s) 496, 746, 1721
AvaI CYCGRG 1 cut(s) 636
AvaII GGWCC 9 cut(s) 136, 280, 391, 523, 633, 670, 711, 1315, 1512
BamHI GGATCC 1 cut(s) 582
BanII GRGCYC 6 cut(s) 53, 318, 495, 681, 827, 1181
BauI CACGAG 1 cut(s) 1675
BbsI GAAGAC 1 cut(s) 560
Bbv12I GWGCWC 5 cut(s) 318, 495, 666, 827, 1181
BbvI GCAGC 8 cut(s) 448, 451, 454, 472, 490, 1174, 1273, 1397
BccI CCATC 6 cut(s) 14, 1067, 1165, 1700, 1737, 1888
BceAI ACGGC 5 cut(s) 373, 394, 1201, 1423, 1506
BcgI CGANNNNNNTGC 2 cut(s) 869, 903
BciVI GTATCC 1 cut(s) 768
BclI TGATCA 3 cut(s) 1309, 1462, 1915
BcnI CCSGG 9 cut(s) 395, 500, 559, 618, 637, 638, 668, 720, 813
BcoDI GTCTC 3 cut(s) 363, 865, 1555
BfaI CTAG 1 cut(s) 110
BfoI RGCGCY 1 cut(s) 1644
BfrI CTTAAG 1 cut(s) 1187
BfuAI ACCTGC 1 cut(s) 1354
BfuI GTATCC 1 cut(s) 768
BglII AGATCT 1 cut(s) 1773
Bme1390I CCNGG 9 cut(s) 395, 500, 559, 618, 637, 638, 668, 720, 813
Bme18I GGWCC 9 cut(s) 136, 280, 391, 523, 633, 670, 711, 1315, 1512
BmeRI GACNNNNNGTC 1 cut(s) 397
BmeT110I CYCGRG 1 cut(s) 636
BmgBI CACGTC 1 cut(s) 286
BmrFI CCNGG 9 cut(s) 395, 500, 559, 618, 637, 638, 668, 720, 813
BmsI GCATC 3 cut(s) 552, 877, 1294
BpiI GAAGAC 1 cut(s) 560
BplI GAGNNNNNCTC 4 cut(s) 1656, 1688, 1867, 1899
BpmI CTGGAG 1 cut(s) 983
BpuEI CTTGAG 1 cut(s) 116
BpuMI CCSGG 9 cut(s) 395, 500, 559, 618, 637, 638, 668, 720, 813
BsaBI GATNNNNATC 2 cut(s) 581, 1308
BsaHI GRCGYC 1 cut(s) 381
BsaJI CCNNGG 8 cut(s) 45, 417, 636, 637, 673, 812, 1232, 1701
BsaWI WCCGGW 1 cut(s) 1279
BsaXI ACNNNNNCTCC 4 cut(s) 275, 305, 714, 744
Bsc4I CCNNNNNNNGG 8 cut(s) 310, 401, 592, 623, 666, 961, 1172, 1819
Bse118I RCCGGY 1 cut(s) 1637
Bse1I ACTGG 2 cut(s) 1393, 1581
Bse3DI GCAATG 4 cut(s) 950, 1420, 1836, 1962
Bse8I GATNNNNATC 2 cut(s) 581, 1308
BseDI CCNNGG 8 cut(s) 45, 417, 636, 637, 673, 812, 1232, 1701
BseGI GGATG 4 cut(s) 567, 892, 1711, 1840
BseJI GATNNNNATC 2 cut(s) 581, 1308
BseLI CCNNNNNNNGG 8 cut(s) 310, 401, 592, 623, 666, 961, 1172, 1819
BseMI GCAATG 4 cut(s) 950, 1420, 1836, 1962
BseMII CTCAG 4 cut(s) 526, 1158, 1784, 1967
BseNI ACTGG 2 cut(s) 1393, 1581
BseRI GAGGAG 4 cut(s) 47, 614, 1003, 1962
BseXI GCAGC 8 cut(s) 448, 451, 454, 472, 490, 1174, 1273, 1397
BseYI CCCAGC 4 cut(s) 175, 200, 206, 890
BsgI GTGCAG 2 cut(s) 479, 1919
Bsh1236I CGCG 5 cut(s) 275, 329, 335, 407, 1792
Bsh1285I CGRYCG 2 cut(s) 264, 485
BshFI GGCC 3 cut(s) 653, 1216, 1231
BsiEI CGRYCG 2 cut(s) 264, 485
BsiHKAI GWGCWC 5 cut(s) 318, 495, 666, 827, 1181
BsiHKCI CYCGRG 1 cut(s) 636
BslFI GGGAC 3 cut(s) 122, 683, 1525
BslI CCNNNNNNNGG 8 cut(s) 310, 401, 592, 623, 666, 961, 1172, 1819
BsmAI GTCTC 3 cut(s) 363, 865, 1555
BsmBI CGTCTC 2 cut(s) 363, 865
BsmFI GGGAC 3 cut(s) 122, 683, 1525
BsnI GGCC 3 cut(s) 653, 1216, 1231
BsoBI CYCGRG 1 cut(s) 636
Bsp1286I GDGCHC 7 cut(s) 53, 318, 495, 666, 681, 827, 1181
Bsp19I CCATGG 2 cut(s) 45, 1701
Bsp68I TCGCGA 1 cut(s) 335
BspANI GGCC 3 cut(s) 653, 1216, 1231
BspCNI CTCAG 4 cut(s) 525, 1159, 1783, 1968
BspFNI CGCG 5 cut(s) 275, 329, 335, 407, 1792
BspMI ACCTGC 1 cut(s) 1354
BspPI GGATC 4 cut(s) 191, 577, 590, 1260
BspQI GCTCTTC 1 cut(s) 323
BspTI CTTAAG 1 cut(s) 1187
BsrDI GCAATG 4 cut(s) 950, 1420, 1836, 1962
BsrFI RCCGGY 1 cut(s) 1637
BsrI ACTGG 2 cut(s) 1393, 1581
BssAI RCCGGY 1 cut(s) 1637
BssECI CCNNGG 8 cut(s) 45, 417, 636, 637, 673, 812, 1232, 1701
BssNAI GTATAC 1 cut(s) 1659
BssNI GRCGYC 1 cut(s) 381
BssSI CACGAG 1 cut(s) 1675
BssT1I CCWWGG 2 cut(s) 45, 1701
Bst1107I GTATAC 1 cut(s) 1659
Bst2BI CACGAG 1 cut(s) 1675
Bst4CI ACNGT 2 cut(s) 674, 692
Bst6I CTCTTC 2 cut(s) 323, 746
BstACI GRCGYC 1 cut(s) 381
BstAFI CTTAAG 1 cut(s) 1187
BstC8I GCNNGC 4 cut(s) 447, 651, 1639, 1686
BstDEI CTNAG 7 cut(s) 512, 1167, 1205, 1244, 1337, 1770, 1976
BstDSI CCRYGG 4 cut(s) 45, 417, 673, 1701
BstEII GGTNACC 2 cut(s) 502, 734
BstF5I GGATG 4 cut(s) 567, 892, 1711, 1840
BstFNI CGCG 5 cut(s) 275, 329, 335, 407, 1792
BstH2I RGCGCY 1 cut(s) 1644
BstHHI GCGC 5 cut(s) 277, 407, 952, 1643, 1792
BstMAI GTCTC 3 cut(s) 363, 865, 1555
BstMCI CGRYCG 2 cut(s) 264, 485
BstMWI GCNNNNNNNGC 4 cut(s) 360, 442, 949, 958
BstNSI RCATGY 1 cut(s) 1637
BstPI GGTNACC 2 cut(s) 502, 734
BstSCI CCNGG 9 cut(s) 393, 498, 557, 616, 635, 636, 666, 718, 811
BstUI CGCG 5 cut(s) 275, 329, 335, 407, 1792
BstV1I GCAGC 8 cut(s) 448, 451, 454, 472, 490, 1174, 1273, 1397
BstV2I GAAGAC 1 cut(s) 560
BstX2I RGATCY 3 cut(s) 582, 1252, 1773
BstYI RGATCY 3 cut(s) 582, 1252, 1773
BstZ17I GTATAC 1 cut(s) 1659
BsuI GTATCC 1 cut(s) 768
BsuRI GGCC 3 cut(s) 653, 1216, 1231
BtgI CCRYGG 4 cut(s) 45, 417, 673, 1701
BtrI CACGTC 1 cut(s) 286
BtsCI GGATG 4 cut(s) 567, 892, 1711, 1840
BtsI GCAGTG 1 cut(s) 1541
BtsIMutI CAGTG 1 cut(s) 1541
BtuMI TCGCGA 1 cut(s) 335
BveI ACCTGC 1 cut(s) 1354
Cac8I GCNNGC 4 cut(s) 447, 651, 1639, 1686
CaiI CAGNNNCTG 2 cut(s) 445, 961
CfoI GCGC 5 cut(s) 277, 407, 952, 1643, 1792
Cfr10I RCCGGY 1 cut(s) 1637
Cfr9I CCCGGG 1 cut(s) 636
CseI GACGC 2 cut(s) 370, 1781
Csp6I GTAC 4 cut(s) 472, 871, 1386, 1878
CviAII CATG 8 cut(s) 46, 754, 779, 1040, 1361, 1559, 1634, 1702
CviQI GTAC 4 cut(s) 472, 871, 1386, 1878
DdeI CTNAG 7 cut(s) 512, 1167, 1205, 1244, 1337, 1770, 1976
DriI GACNNNNNGTC 1 cut(s) 397
Eam1104I CTCTTC 2 cut(s) 323, 746
Eam1105I GACNNNNNGTC 1 cut(s) 397
EarI CTCTTC 2 cut(s) 323, 746
EciI GGCGGA 4 cut(s) 82, 381, 464, 611
Ecl136II GAGCTC 4 cut(s) 316, 493, 825, 1179
Eco130I CCWWGG 2 cut(s) 45, 1701
Eco24I GRGCYC 6 cut(s) 53, 318, 495, 681, 827, 1181
Eco47I GGWCC 9 cut(s) 136, 280, 391, 523, 633, 670, 711, 1315, 1512
Eco53kI GAGCTC 4 cut(s) 316, 493, 825, 1179
Eco88I CYCGRG 1 cut(s) 636
Eco91I GGTNACC 2 cut(s) 502, 734
EcoICRI GAGCTC 4 cut(s) 316, 493, 825, 1179
EcoO109I RGGNCCY 3 cut(s) 136, 1315, 1512
EcoO65I GGTNACC 2 cut(s) 502, 734
EcoT14I CCWWGG 2 cut(s) 45, 1701
EcoT22I ATGCAT 1 cut(s) 999
EcoT38I GRGCYC 6 cut(s) 53, 318, 495, 681, 827, 1181
ErhI CCWWGG 2 cut(s) 45, 1701
Esp3I CGTCTC 2 cut(s) 363, 865
FaeI CATG 8 cut(s) 49, 757, 782, 1043, 1364, 1562, 1637, 1705
FaqI GGGAC 3 cut(s) 122, 683, 1525
FatI CATG 8 cut(s) 45, 753, 778, 1039, 1360, 1558, 1633, 1701
FauI CCCGC 4 cut(s) 483, 642, 860, 1539
FbaI TGATCA 3 cut(s) 1309, 1462, 1915
FblI GTMKAC 2 cut(s) 288, 1658
FokI GGATG 4 cut(s) 574, 899, 1718, 1847
FriOI GRGCYC 6 cut(s) 53, 318, 495, 681, 827, 1181
FspAI RTGCGCAY 1 cut(s) 951
FspBI CTAG 1 cut(s) 110
FspI TGCGCA 1 cut(s) 951
GlaI GCGC 5 cut(s) 276, 406, 951, 1642, 1791
GsaI CCCAGC 4 cut(s) 179, 204, 210, 894
GsuI CTGGAG 1 cut(s) 983
HaeII RGCGCY 1 cut(s) 1644
HaeIII GGCC 3 cut(s) 653, 1216, 1231
HgaI GACGC 2 cut(s) 370, 1781
HhaI GCGC 5 cut(s) 277, 407, 952, 1643, 1792
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 8 cut(s) 49, 757, 782, 1043, 1364, 1562, 1637, 1705
Hin6I GCGC 5 cut(s) 275, 405, 950, 1641, 1790
HinP1I GCGC 5 cut(s) 275, 405, 950, 1641, 1790
HincII GTYRAC 1 cut(s) 1405
HindII GTYRAC 1 cut(s) 1405
HindIII AAGCTT 1 cut(s) 1142
HpaI GTTAAC 1 cut(s) 1405
HphI GGTGA 3 cut(s) 496, 746, 1721
Hpy166II GTNNAC 3 cut(s) 289, 1405, 1659
Hpy188III TCNNGA 8 cut(s) 68, 334, 389, 550, 1091, 1313, 1675, 1781
Hpy8I GTNNAC 3 cut(s) 289, 1405, 1659
Hpy99I CGWCG 6 cut(s) 173, 269, 359, 386, 521, 850
HpyAV CCTTC 2 cut(s) 202, 1525
HpyCH4III ACNGT 2 cut(s) 674, 692
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 7 cut(s) 460, 997, 1349, 1413, 1829, 1900, 1955
HpyF10VI GCNNNNNNNGC 4 cut(s) 360, 442, 949, 958
HpyF3I CTNAG 7 cut(s) 512, 1167, 1205, 1244, 1337, 1770, 1976
HpySE526I ACGT 1 cut(s) 285
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 8 cut(s) 49, 757, 782, 1043, 1364, 1562, 1637, 1705
HspAI GCGC 5 cut(s) 275, 405, 950, 1641, 1790
KroI GCCGGC 1 cut(s) 1637
KroNI GCCGGC 1 cut(s) 1639
Ksp22I TGATCA 3 cut(s) 1309, 1462, 1915
KspAI GTTAAC 1 cut(s) 1405
LguI GCTCTTC 1 cut(s) 323
Lsp1109I GCAGC 8 cut(s) 448, 451, 454, 472, 490, 1174, 1273, 1397
LweI GCATC 3 cut(s) 552, 877, 1294
MaeI CTAG 1 cut(s) 110
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 3 cut(s) 502, 734, 2042
MboII GAAGA 9 cut(s) 310, 478, 565, 622, 733, 736, 800, 1045, 1285
MflI RGATCY 3 cut(s) 582, 1252, 1773
MhlI GDGCHC 7 cut(s) 53, 318, 495, 666, 681, 827, 1181
MluCI AATT 5 cut(s) 63, 100, 798, 1420, 1692
MlyI GAGTC 3 cut(s) 407, 732, 1210
MmeI TCCRAC 5 cut(s) 237, 963, 1379, 1520, 1820
Mph1103I ATGCAT 1 cut(s) 999
MroNI GCCGGC 1 cut(s) 1637
MseI TTAA 4 cut(s) 1140, 1188, 1404, 1985
MspA1I CMGCKG 4 cut(s) 445, 463, 481, 1819
MspCI CTTAAG 1 cut(s) 1187
MspR9I CCNGG 9 cut(s) 395, 500, 559, 618, 637, 638, 668, 720, 813
MvnI CGCG 5 cut(s) 275, 329, 335, 407, 1792
MwoI GCNNNNNNNGC 4 cut(s) 360, 442, 949, 958
NaeI GCCGGC 1 cut(s) 1639
NciI CCSGG 9 cut(s) 395, 500, 559, 618, 637, 638, 668, 720, 813
NcoI CCATGG 2 cut(s) 45, 1701
NgoMIV GCCGGC 1 cut(s) 1637
NlaIII CATG 8 cut(s) 49, 757, 782, 1043, 1364, 1562, 1637, 1705
NmeAIII GCCGAG 2 cut(s) 1222, 1257
NmuCI GTSAC 2 cut(s) 502, 734
NruI TCGCGA 1 cut(s) 335
NsbI TGCGCA 1 cut(s) 951
NsiI ATGCAT 1 cut(s) 999
NspI RCATGY 1 cut(s) 1637
PaqCI CACCTGC 1 cut(s) 1354
PciSI GCTCTTC 1 cut(s) 323
PcsI WCGNNNNNNNCGW 2 cut(s) 291, 378
PdiI GCCGGC 1 cut(s) 1639
PfeI GAWTC 9 cut(s) 343, 572, 577, 613, 1036, 1087, 1152, 1276, 1543
PflFI GACNNNGTC 2 cut(s) 284, 380
PflMI CCANNNNNTGG 1 cut(s) 961
PfoI TCCNGGA 2 cut(s) 666, 718
Ple19I CGATCG 1 cut(s) 264
PleI GAGTC 3 cut(s) 406, 731, 1209
PpsI GAGTC 3 cut(s) 406, 731, 1209
PpuMI RGGWCCY 3 cut(s) 136, 1315, 1512
PsiI TTATAA 1 cut(s) 1134
Psp124BI GAGCTC 4 cut(s) 318, 495, 827, 1181
Psp5II RGGWCCY 3 cut(s) 136, 1315, 1512
PspEI GGTNACC 2 cut(s) 502, 734
PspFI CCCAGC 4 cut(s) 175, 200, 206, 890
PspPPI RGGWCCY 3 cut(s) 136, 1315, 1512
PstNI CAGNNNCTG 2 cut(s) 445, 961
PsuI RGATCY 3 cut(s) 582, 1252, 1773
PsyI GACNNNGTC 2 cut(s) 284, 380
PvuI CGATCG 1 cut(s) 264
PvuII CAGCTG 1 cut(s) 445
RruI TCGCGA 1 cut(s) 335
RsaI GTAC 4 cut(s) 473, 872, 1387, 1879
RsaNI GTAC 4 cut(s) 472, 871, 1386, 1878
SacI GAGCTC 4 cut(s) 318, 495, 827, 1181
SapI GCTCTTC 1 cut(s) 323
SaqAI TTAA 4 cut(s) 1140, 1188, 1404, 1985
SchI GAGTC 3 cut(s) 407, 732, 1210
ScrFI CCNGG 9 cut(s) 395, 500, 559, 618, 637, 638, 668, 720, 813
SduI GDGCHC 7 cut(s) 53, 318, 495, 666, 681, 827, 1181
SfaNI GCATC 3 cut(s) 552, 877, 1294
SinI GGWCC 9 cut(s) 136, 280, 391, 523, 633, 670, 711, 1315, 1512
SmaI CCCGGG 1 cut(s) 638
SmlI CTYRAG 2 cut(s) 131, 1187
SmoI CTYRAG 2 cut(s) 131, 1187
Sse9I AATT 5 cut(s) 63, 100, 798, 1420, 1692
SspMI CTAG 1 cut(s) 110
SstI GAGCTC 4 cut(s) 318, 495, 827, 1181
StyD4I CCNGG 9 cut(s) 393, 498, 557, 616, 635, 636, 666, 718, 811
StyI CCWWGG 2 cut(s) 45, 1701
TaaI ACNGT 2 cut(s) 674, 692
TaiI ACGT 1 cut(s) 288
TaqI TCGA 8 cut(s) 147, 195, 260, 485, 575, 827, 1052, 1155
TasI AATT 5 cut(s) 63, 100, 798, 1420, 1692
TauI GCSGC 5 cut(s) 272, 275, 363, 366, 656
TfiI GAWTC 9 cut(s) 343, 572, 577, 613, 1036, 1087, 1152, 1276, 1543
Tru1I TTAA 4 cut(s) 1140, 1188, 1404, 1985
Tru9I TTAA 4 cut(s) 1140, 1188, 1404, 1985
TscAI CASTG 1 cut(s) 1541
TseFI GTSAC 2 cut(s) 502, 734
TseI GCWGC 8 cut(s) 436, 439, 442, 460, 478, 1162, 1261, 1410
Tsp45I GTSAC 2 cut(s) 502, 734
TspDTI ATGAA 4 cut(s) 1028, 1431, 1451, 2031
TspGWI ACGGA 4 cut(s) 527, 646, 649, 933
TspMI CCCGGG 1 cut(s) 636
TspRI CASTG 1 cut(s) 1541
Tth111I GACNNNGTC 2 cut(s) 284, 380
Van91I CCANNNNNTGG 1 cut(s) 961
Vha464I CTTAAG 1 cut(s) 1187
VpaK11BI GGWCC 9 cut(s) 136, 280, 391, 523, 633, 670, 711, 1315, 1512
XapI RAATTY 1 cut(s) 1692
XceI RCATGY 1 cut(s) 1637
XmaI CCCGGG 1 cut(s) 636
XmiI GTMKAC 2 cut(s) 288, 1658
XspI CTAG 1 cut(s) 110
Zsp2I ATGCAT 1 cut(s) 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.