Rmu_sc0014821.1_g000001

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0014821.1
OG0000336
Physical Location & Seq
Reverse (-)
2444 .. 3771
1328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0014821.1_g000001.1.cds

Sequence Viewer

Length: 960 bp
atggaggaggtggatgagatggttgttcgactggcagcttctattagaccggcagatattagcaggccggtggatcatcgtccagcttatggagagggtttgcgtcgttcccagacgtatctggtaggaaagttgttgacggctagggccttcaataagagatcattcatgggcatgttccgtcttgcatggaggttgaatggctggtatactgctcaggaacatggagaccgctttctgttcaccttgacggatccaacaaatcgtaacaggatccttcgaggtggtccttggggttttgatagagctccggttgtcctaggaccgtatgatgggatggggacgatcaaggatgttctgctctctactttagccttttggatcaaggtacgtggtctgccaccagattttcatactgatcgacgtcaagaatgtggtttgattactcatgtggtgggacgccgctgctggtggaggccaggactgagtcggcagaatcttgaaagggttttgaaccgcgtggagttgtctccacatgttggaccatcgatggtgtttggagcacagctgagtccacaaagcgagcaagtggagagtggtagtgaaccatcttcgggaggtgtagggcatgagctgattgggaggggtagttcatgtgagaagctgcagttgagcttagcagtgggcagaaaaaatttggatgctatgacaatgggattgagttctgtgaaagctgtgaagaaagtatacaagaagaggccgggacggccaaagggggcaaagaataaggcaaaggagcttaaggttgtgcatgcttcacctatgaagaagtcaccggcaaagaagctcaagactcctaggaaggaggagaaattggtgaacggactacaggaattcaccgtgatggaggcagctcatggttccaacccatcacggggtaaggagccaatagtagtttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

319

Amino Acids

35.55

Weight (kDa)

10.31

Isoelectric Point (pI)

47.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000336)

Species Orthologous Gene IDs
malus_domestica MD05G1024100.v1.1
rosa_chinensis RchiOBHm_Chr2g0152291 RchiOBHm_Chr4g0387411
rosa_laevigata RLG00000009698 RLG00000009699 RLG00000009700 RLG00000023615 RLG00000024265 RLG00000034445 RLG00000036567
rosa_multiflora Rmu_co8268067.1_g000001 Rmu_co8313555.1_g000001 Rmu_co8324697.1_g000001 Rmu_sc0000318.1_g000023 Rmu_sc0000342.1_g000016 Rmu_sc0000447.1_g000010 Rmu_sc0000528.1_g000009 Rmu_sc0000539.1_g000060 Rmu_sc0000929.1_g000023 Rmu_sc0001493.1_g000081 Rmu_sc0001674.1_g000003 Rmu_sc0001791.1_g000023 Rmu_sc0001822.1_g000005 Rmu_sc0001822.1_g000033 Rmu_sc0002061.1_g000010 Rmu_sc0002286.1_g000012 Rmu_sc0002979.1_g000009 Rmu_sc0003158.1_g000016 Rmu_sc0003221.1_g000001 Rmu_sc0003641.1_g000029 Rmu_sc0003742.1_g000012 Rmu_sc0003814.1_g000004 Rmu_sc0003823.1_g000005 Rmu_sc0004002.1_g000005 Rmu_sc0004564.1_g000005 Rmu_sc0005415.1_g000003 Rmu_sc0006050.1_g000025 Rmu_sc0006555.1_g000002 Rmu_sc0009954.1_g000020 Rmu_sc0010421.1_g000007 Rmu_sc0010607.1_g000002 Rmu_sc0011044.1_g000005 Rmu_sc0011497.1_g000015 Rmu_sc0014532.1_g000004 Rmu_sc0014745.1_g000005 Rmu_sc0014821.1_g000001 Rmu_sc0015038.1_g000002 Rmu_sc0015454.1_g000004 Rmu_sc0019663.1_g000002 Rmu_sc0027475.1_g000003 Rmu_sc0028826.1_g000002 Rmu_ssc0000117.1_g000019 Rmu_ssc0000175.1_g000034
rosa_roxburghii Rroxscaffold_3G00237080 Rroxscaffold_4G00288290 Rroxscaffold_6G00397510
rosa_rugosa Rorug01G0035000 Rorug01G0190900 Rorug01G0208500 Rorug01G0208600 Rorug01G0208600 Rorug01G0344900 Rorug01G0479500 Rorug01G0479600 Rorug02G0230400 Rorug02G0320900 Rorug02G0433300 Rorug03G0147400 Rorug03G0169700.1 Rorug03G0169800 Rorug03G0198300 Rorug04G0022900 Rorug04G0064600 Rorug04G0322100 Rorug04G0388900 Rorug05G0153200 Rorug05G0231600 Rorug05G0231700 Rorug05G0231800 Rorug05G0276200 Rorug05G0292400 Rorug05G0314700 Rorug06G0092000 Rorug06G0260900 Rorug06G0273600 RorugPtG0003200
rosa_samantha Rh1AG264100 Rh1AG292500 Rh1AG423800 Rh2AG098000 Rh2AG232700 Rh2AG361100 Rh2AG372900 Rh2BG099200 Rh2BG246000 Rh2BG260600 Rh2BG378400 Rh3BG142300 Rh3BG252900 Rh3BG267700 Rh3DG302400 Rh5BG059800 Rh5BG320000 Rh5BG530500 Rh6AG089300 Rh6AG089400 Rh6AG127400 Rh6AG376700 Rh7CG488700 Rh7DG265900
rosa_wichuraiana Rw2G040640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 427
AccB7I CCANNNNNTGG 2 cut(s) 89, 539
AccI GTMKAC 2 cut(s) 209, 747
AccII CGCG 1 cut(s) 519
AciI CCGC 3 cut(s) 232, 463, 517
AclWI GGATC 6 cut(s) 81, 248, 261, 268, 281, 389
AcoI YGGCCR 1 cut(s) 767
AcsI RAATTY 2 cut(s) 694, 893
AcyI GRCGYC 2 cut(s) 424, 460
AfaI GTAC 1 cut(s) 390
AfiI CCNNNNNNNGG 6 cut(s) 89, 332, 539, 614, 934, 935
AflII CTTAAG 1 cut(s) 800
AflIII ACRYGT 1 cut(s) 535
AgsI TTSAA 4 cut(s) 154, 199, 503, 514
AjnI CCWGG 1 cut(s) 478
AloI GAACNNNNNNTCC 2 cut(s) 634, 666
Alw21I GWGCWC 2 cut(s) 310, 565
Alw26I GTCTC 2 cut(s) 222, 534
AlwI GGATC 6 cut(s) 81, 248, 261, 268, 281, 389
AoxI GGCC 5 cut(s) 65, 147, 476, 758, 767
ApeKI GCWGC 4 cut(s) 35, 465, 664, 911
ApoI RAATTY 2 cut(s) 694, 893
AspA2I CCTAGG 2 cut(s) 319, 857
AspS9I GGNCC 4 cut(s) 147, 287, 323, 542
AsuC2I CCSGG 1 cut(s) 762
AsuHPI GGTGA 5 cut(s) 235, 810, 825, 889, 889
AvaII GGWCC 3 cut(s) 287, 323, 542
AvrII CCTAGG 2 cut(s) 319, 857
BamHI GGATCC 2 cut(s) 253, 273
BanII GRGCYC 1 cut(s) 310
BarI GAAGNNNNNNTAC 2 cut(s) 731, 763
Bbv12I GWGCWC 2 cut(s) 310, 565
BbvI GCAGC 4 cut(s) 47, 452, 651, 923
BccI CCATC 8 cut(s) 13, 326, 331, 544, 553, 616, 898, 937
BceAI ACGGC 2 cut(s) 156, 782
BciT130I CCWGG 1 cut(s) 480
BcnI CCSGG 1 cut(s) 762
BcoDI GTCTC 2 cut(s) 222, 534
BfaI CTAG 3 cut(s) 144, 320, 858
BfmI CTRYAG 2 cut(s) 665, 887
BfrI CTTAAG 1 cut(s) 800
BglI GCCNNNNNGGC 1 cut(s) 766
BisI GCNGC 5 cut(s) 36, 463, 466, 665, 912
BlnI CCTAGG 2 cut(s) 319, 857
BlpI GCTNAGC 1 cut(s) 676
BlsI GCNGC 5 cut(s) 37, 464, 467, 666, 913
Bme1390I CCNGG 2 cut(s) 480, 762
Bme18I GGWCC 3 cut(s) 287, 323, 542
BmgT120I GGNCC 4 cut(s) 147, 287, 323, 542
BmiI GGNNCC 4 cut(s) 255, 275, 922, 945
BmrFI CCNGG 2 cut(s) 480, 762
BmsI GCATC 1 cut(s) 691
Bpu10I CCTNAGC 1 cut(s) 216
Bpu1102I GCTNAGC 1 cut(s) 676
BpuEI CTTGAG 1 cut(s) 833
BpuMI CCSGG 1 cut(s) 762
Bsa29I ATCGAT 1 cut(s) 548
BsaAI YACGTR 1 cut(s) 392
BsaHI GRCGYC 2 cut(s) 424, 460
BsaI GGTCTC 1 cut(s) 222
BsaJI CCNNGG 3 cut(s) 290, 319, 857
BsaWI WCCGGW 1 cut(s) 310
BsaXI ACNNNNNCTCC 2 cut(s) 634, 664
Bsc4I CCNNNNNNNGG 6 cut(s) 89, 332, 539, 614, 934, 935
Bse118I RCCGGY 3 cut(s) 49, 67, 835
Bse1I ACTGG 1 cut(s) 36
BseBI CCWGG 1 cut(s) 480
BseCI ATCGAT 1 cut(s) 548
BseDI CCNNGG 3 cut(s) 290, 319, 857
BseGI GGATG 4 cut(s) 19, 342, 358, 706
BseLI CCNNNNNNNGG 6 cut(s) 89, 332, 539, 614, 934, 935
BseMII CTCAG 3 cut(s) 230, 476, 560
BseNI ACTGG 1 cut(s) 36
BseRI GAGGAG 2 cut(s) 20, 881
BseXI GCAGC 4 cut(s) 47, 452, 651, 923
Bsh1236I CGCG 1 cut(s) 519
BshFI GGCC 5 cut(s) 67, 149, 478, 760, 769
BshVI ATCGAT 1 cut(s) 548
BsiHKAI GWGCWC 2 cut(s) 310, 565
BsiSI CCGG 5 cut(s) 50, 68, 311, 761, 836
BslFI GGGAC 3 cut(s) 355, 471, 777
BslI CCNNNNNNNGG 6 cut(s) 89, 332, 539, 614, 934, 935
BsmAI GTCTC 2 cut(s) 222, 534
BsmFI GGGAC 3 cut(s) 355, 471, 777
BsnI GGCC 5 cut(s) 67, 149, 478, 760, 769
Bso31I GGTCTC 1 cut(s) 222
Bsp1286I GDGCHC 2 cut(s) 310, 565
Bsp143I GATC 7 cut(s) 73, 161, 253, 273, 345, 381, 418
Bsp1720I GCTNAGC 1 cut(s) 676
BspACI CCGC 3 cut(s) 232, 463, 517
BspANI GGCC 5 cut(s) 67, 149, 478, 760, 769
BspCNI CTCAG 3 cut(s) 229, 477, 561
BspDI ATCGAT 1 cut(s) 548
BspFNI CGCG 1 cut(s) 519
BspLI GGNNCC 4 cut(s) 255, 275, 922, 945
BspMAI CTGCAG 1 cut(s) 669
BspPI GGATC 6 cut(s) 81, 248, 261, 268, 281, 389
BspTI CTTAAG 1 cut(s) 800
BspTNI GGTCTC 1 cut(s) 222
BsrFI RCCGGY 3 cut(s) 49, 67, 835
BsrI ACTGG 1 cut(s) 36
BssAI RCCGGY 3 cut(s) 49, 67, 835
BssECI CCNNGG 3 cut(s) 290, 319, 857
BssMI GATC 7 cut(s) 73, 161, 253, 273, 345, 381, 418
BssNAI GTATAC 2 cut(s) 210, 748
BssNI GRCGYC 2 cut(s) 424, 460
BssT1I CCWWGG 3 cut(s) 290, 319, 857
Bst1107I GTATAC 2 cut(s) 210, 748
Bst2UI CCWGG 1 cut(s) 480
Bst4CI ACNGT 2 cut(s) 327, 901
Bst6I CTCTTC 1 cut(s) 749
BstACI GRCGYC 2 cut(s) 424, 460
BstAFI CTTAAG 1 cut(s) 800
BstBAI YACGTR 1 cut(s) 392
BstC8I GCNNGC 3 cut(s) 65, 584, 813
BstDEI CTNAG 4 cut(s) 216, 485, 569, 676
BstF5I GGATG 4 cut(s) 19, 342, 358, 706
BstFNI CGCG 1 cut(s) 519
BstKTI GATC 7 cut(s) 76, 164, 256, 276, 348, 384, 421
BstMAI GTCTC 2 cut(s) 222, 534
BstMBI GATC 7 cut(s) 73, 161, 253, 273, 345, 381, 418
BstMWI GCNNNNNNNGC 1 cut(s) 766
BstNI CCWGG 1 cut(s) 480
BstNSI RCATGY 3 cut(s) 178, 539, 815
BstSCI CCNGG 2 cut(s) 478, 760
BstSFI CTRYAG 2 cut(s) 665, 887
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 4 cut(s) 47, 452, 651, 923
BstX2I RGATCY 2 cut(s) 253, 273
BstYI RGATCY 2 cut(s) 253, 273
BstZ17I GTATAC 2 cut(s) 210, 748
Bsu15I ATCGAT 1 cut(s) 548
BsuRI GGCC 5 cut(s) 67, 149, 478, 760, 769
BsuTUI ATCGAT 1 cut(s) 548
BtsCI GGATG 4 cut(s) 19, 342, 358, 706
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 1 cut(s) 687
Cac8I GCNNGC 3 cut(s) 65, 584, 813
Cfr10I RCCGGY 3 cut(s) 49, 67, 835
Cfr13I GGNCC 4 cut(s) 147, 287, 323, 542
ClaI ATCGAT 1 cut(s) 548
CseI GACGC 2 cut(s) 92, 468
Csp6I GTAC 1 cut(s) 389
CspCI CAANNNNNGTGG 2 cut(s) 373, 408
CviQI GTAC 1 cut(s) 389
DdeI CTNAG 4 cut(s) 216, 485, 569, 676
DpnI GATC 7 cut(s) 75, 163, 255, 275, 347, 383, 420
DpnII GATC 7 cut(s) 73, 161, 253, 273, 345, 381, 418
EaeI YGGCCR 1 cut(s) 767
Eam1104I CTCTTC 1 cut(s) 749
EarI CTCTTC 1 cut(s) 749
Ecl136II GAGCTC 1 cut(s) 308
Eco130I CCWWGG 3 cut(s) 290, 319, 857
Eco24I GRGCYC 1 cut(s) 310
Eco31I GGTCTC 1 cut(s) 222
Eco47I GGWCC 3 cut(s) 287, 323, 542
Eco53kI GAGCTC 1 cut(s) 308
EcoICRI GAGCTC 1 cut(s) 308
EcoO109I RGGNCCY 1 cut(s) 147
EcoRI GAATTC 1 cut(s) 893
EcoRII CCWGG 1 cut(s) 478
EcoT14I CCWWGG 3 cut(s) 290, 319, 857
EcoT38I GRGCYC 1 cut(s) 310
ErhI CCWWGG 3 cut(s) 290, 319, 857
FaqI GGGAC 3 cut(s) 355, 471, 777
FblI GTMKAC 2 cut(s) 209, 747
Fnu4HI GCNGC 5 cut(s) 36, 463, 466, 665, 912
FokI GGATG 4 cut(s) 26, 349, 365, 713
FriOI GRGCYC 1 cut(s) 310
Fsp4HI GCNGC 5 cut(s) 36, 463, 466, 665, 912
FspBI CTAG 3 cut(s) 144, 320, 858
GluI GCNGC 5 cut(s) 36, 463, 466, 665, 912
HaeIII GGCC 5 cut(s) 67, 149, 478, 760, 769
HapII CCGG 5 cut(s) 50, 68, 311, 761, 836
HgaI GACGC 2 cut(s) 92, 468
Hin1I GRCGYC 2 cut(s) 424, 460
HincII GTYRAC 1 cut(s) 138
HindII GTYRAC 1 cut(s) 138
HinfI GANTC 4 cut(s) 487, 496, 571, 853
HpaII CCGG 5 cut(s) 50, 68, 311, 761, 836
HphI GGTGA 5 cut(s) 235, 810, 825, 889, 889
Hpy166II GTNNAC 7 cut(s) 138, 210, 243, 575, 605, 748, 880
Hpy188III TCNNGA 5 cut(s) 218, 428, 500, 615, 850
Hpy8I GTNNAC 7 cut(s) 138, 210, 243, 575, 605, 748, 880
Hpy99I CGWCG 2 cut(s) 108, 426
HpyAV CCTTC 3 cut(s) 160, 287, 856
HpyCH4III ACNGT 2 cut(s) 327, 901
HpyCH4IV ACGT 3 cut(s) 116, 391, 424
HpyCH4V TGCA 3 cut(s) 188, 667, 811
HpyF10VI GCNNNNNNNGC 1 cut(s) 766
HpyF3I CTNAG 4 cut(s) 216, 485, 569, 676
HpySE526I ACGT 3 cut(s) 116, 391, 424
Hsp92I GRCGYC 2 cut(s) 424, 460
Kzo9I GATC 7 cut(s) 73, 161, 253, 273, 345, 381, 418
LmnI GCTCC 4 cut(s) 313, 560, 796, 943
Lsp1109I GCAGC 4 cut(s) 47, 452, 651, 923
LweI GCATC 1 cut(s) 691
MaeI CTAG 3 cut(s) 144, 320, 858
MaeII ACGT 3 cut(s) 116, 391, 424
MaeIII GTNAC 2 cut(s) 266, 831
MalI GATC 7 cut(s) 75, 163, 255, 275, 347, 383, 420
MboI GATC 7 cut(s) 73, 161, 253, 273, 345, 381, 418
MboII GAAGA 4 cut(s) 603, 751, 766, 838
MflI RGATCY 2 cut(s) 253, 273
MhlI GDGCHC 2 cut(s) 310, 565
MluCI AATT 3 cut(s) 694, 872, 893
MlyI GAGTC 3 cut(s) 496, 580, 847
MmeI TCCRAC 3 cut(s) 281, 520, 948
MnlI CCTC 9 cut(s) 88, 186, 275, 468, 611, 636, 750, 859, 901
MseI TTAA 1 cut(s) 801
MslI CAYNNNNRTG 2 cut(s) 173, 902
MspA1I CMGCKG 2 cut(s) 465, 568
MspCI CTTAAG 1 cut(s) 800
MspI CCGG 5 cut(s) 50, 68, 311, 761, 836
MspR9I CCNGG 2 cut(s) 480, 762
MvaI CCWGG 1 cut(s) 480
MvnI CGCG 1 cut(s) 519
MwoI GCNNNNNNNGC 1 cut(s) 766
NciI CCSGG 1 cut(s) 762
NdeII GATC 7 cut(s) 73, 161, 253, 273, 345, 381, 418
NlaIV GGNNCC 4 cut(s) 255, 275, 922, 945
NmuCI GTSAC 1 cut(s) 831
NspI RCATGY 3 cut(s) 178, 539, 815
PaeI GCATGC 1 cut(s) 815
PciI ACATGT 1 cut(s) 535
PfeI GAWTC 1 cut(s) 496
PflFI GACNNNGTC 1 cut(s) 486
PflMI CCANNNNNTGG 2 cut(s) 89, 539
PkrI GCNGC 5 cut(s) 37, 464, 467, 666, 913
PleI GAGTC 3 cut(s) 495, 579, 847
PpsI GAGTC 3 cut(s) 495, 579, 847
Ppu21I YACGTR 1 cut(s) 392
PscI ACATGT 1 cut(s) 535
Psp124BI GAGCTC 1 cut(s) 310
Psp6I CCWGG 1 cut(s) 478
PspGI CCWGG 1 cut(s) 478
PspN4I GGNNCC 4 cut(s) 255, 275, 922, 945
PspPI GGNCC 4 cut(s) 147, 287, 323, 542
PstI CTGCAG 1 cut(s) 669
PsuI RGATCY 2 cut(s) 253, 273
PsyI GACNNNGTC 1 cut(s) 486
PvuII CAGCTG 1 cut(s) 568
RsaI GTAC 1 cut(s) 390
RsaNI GTAC 1 cut(s) 389
RseI CAYNNNNRTG 2 cut(s) 173, 902
SacI GAGCTC 1 cut(s) 310
SaqAI TTAA 1 cut(s) 801
SatI GCNGC 5 cut(s) 36, 463, 466, 665, 912
Sau3AI GATC 7 cut(s) 73, 161, 253, 273, 345, 381, 418
Sau96I GGNCC 4 cut(s) 147, 287, 323, 542
SchI GAGTC 3 cut(s) 496, 580, 847
ScrFI CCNGG 2 cut(s) 480, 762
SduI GDGCHC 2 cut(s) 310, 565
SfaNI GCATC 1 cut(s) 691
SfcI CTRYAG 2 cut(s) 665, 887
SfiI GGCCNNNNNGGCC 1 cut(s) 766
SinI GGWCC 3 cut(s) 287, 323, 542
SmiMI CAYNNNNRTG 2 cut(s) 173, 902
SmlI CTYRAG 2 cut(s) 800, 848
SmoI CTYRAG 2 cut(s) 800, 848
SphI GCATGC 1 cut(s) 815
Sse9I AATT 3 cut(s) 694, 872, 893
SsiI CCGC 3 cut(s) 232, 463, 517
SspMI CTAG 3 cut(s) 144, 320, 858
SstI GAGCTC 1 cut(s) 310
StyD4I CCNGG 2 cut(s) 478, 760
StyI CCWWGG 3 cut(s) 290, 319, 857
TaaI ACNGT 2 cut(s) 327, 901
TaiI ACGT 3 cut(s) 119, 394, 427
TaqI TCGA 4 cut(s) 28, 280, 421, 548
TasI AATT 3 cut(s) 694, 872, 893
TauI GCSGC 1 cut(s) 465
TfiI GAWTC 1 cut(s) 496
Tru1I TTAA 1 cut(s) 801
Tru9I TTAA 1 cut(s) 801
TscAI CASTG 1 cut(s) 687
TseFI GTSAC 1 cut(s) 831
TseI GCWGC 4 cut(s) 35, 465, 664, 911
Tsp45I GTSAC 1 cut(s) 831
TspDTI ATGAA 4 cut(s) 157, 401, 642, 839
TspGWI ACGGA 3 cut(s) 170, 266, 897
TspRI CASTG 1 cut(s) 687
Tth111I GACNNNGTC 1 cut(s) 486
Van91I CCANNNNNTGG 2 cut(s) 89, 539
Vha464I CTTAAG 1 cut(s) 800
VpaK11BI GGWCC 3 cut(s) 287, 323, 542
XapI RAATTY 2 cut(s) 694, 893
XceI RCATGY 3 cut(s) 178, 539, 815
XmaJI CCTAGG 2 cut(s) 319, 857
XmiI GTMKAC 2 cut(s) 209, 747
XspI CTAG 3 cut(s) 144, 320, 858
ZraI GACGTC 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.