RchiOBHm_Chr2g0152291

Zinc knuckle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000336
Physical Location & Seq
Forward (+)
69696319 .. 69698764
2446 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52145

Sequence Viewer

Length: 1188 bp
ATGATTGTCACATATTTCAGTCTAGCTAACTATCATTTAACTTGCCTTTTCAAAAAAAAGAAAAAGACTCGAGTCGACTCCATTCATTGGTTAAGTGTTGGAGCTATCAAAGATGTGCCGCTTAATACACTTGCTTTCTGGATCAAGGTGCGTGGACTATCGCCGGATTTTCATACGGATCGCTATCTTAGGAGGATAGGGAGTGCCATAGGTCGGTTTGTGCAGAAGGACACCACAAAGTTTGCTGCAGGACAAATCCGGATTCGGGTTGAGATGGACCTGCTGCAGCCTATCATCTTTCAGCGTAGATTCACGGTAGATGATGGTATTGTGGTGGATTTGGAATTCTTCTTCGAAAATTTGTATGGGCGGTGCAAGGATTGTGGACTTGTCACTCATGTTGGCTTACCGTGTTCGGAAACTCCTTTGCAGGCAGAGCTGCAAACTGATACTCCTTTAGCACGTCAAAATCTGGAGCGGGATTTGAACCAAGTCGGAGTGATACAGCCGGCTGGGCCAATGTTGGTTTTTGGGGCACAGAATTCCCCCCAGGTTGGGTTAGAGAGGTTTATGCCGACTGTTAAACCTAAAGTGAGGAAAGAAATACTTTGGAGGCCTTCTGTTGGCAGTGGAGATGGTAGCTCTGGGGTGCAGACAGACTCAAATGAGGTGGAGCTTGCTGGTGGTAGTGAACCTAAGTCTGATGGCTCAGGTAAGGAGACATTTGGAGCAAGCAGTTCGACAGATCATGCACCGAATCCTGCAATGCATTCTCCTGGTCGTGGACTGCGTCGTAAGCGTGTGGATGAGGATGGGATTTCTCCAAAGAAAATGAGATTGAGCTTGGCAGTTGGAAGAACTAATCTGGATGCTTTGACCATGGGTTTGTGCAATGTGAAGCCGGCTGAGAAGGTATACAAGAAGAGAGGTAGGTCGAAAGGTACAAAAAACAACACAAAGGAGTTCAAGGTAGTTCAAGCTTCTCCTATGAAAAAGTCTCCATCAAAGAAGAGTAAGTCTCCACGAAAAGTGGAAAAGTTGGTGAATGGTTTTCCGGAATTTACTTTAGGTGAGGTTGTCAATGGTTCTAATGGGAAGGAACCTATATCTTCTGTACTATTCTTGATCTATGAACCGGGACATACTATTGGTATGTTTCCATTTTATAAAAAAAAAAGTGTTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

395

Amino Acids

43.83

Weight (kDa)

9.86

Isoelectric Point (pI)

34.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 92 - 138 1e-05 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000336)

Species Orthologous Gene IDs
malus_domestica MD05G1024100.v1.1
rosa_chinensis RchiOBHm_Chr2g0152291 RchiOBHm_Chr4g0387411
rosa_laevigata RLG00000009698 RLG00000009699 RLG00000009700 RLG00000023615 RLG00000024265 RLG00000034445 RLG00000036567
rosa_multiflora Rmu_co8268067.1_g000001 Rmu_co8313555.1_g000001 Rmu_co8324697.1_g000001 Rmu_sc0000318.1_g000023 Rmu_sc0000342.1_g000016 Rmu_sc0000447.1_g000010 Rmu_sc0000528.1_g000009 Rmu_sc0000539.1_g000060 Rmu_sc0000929.1_g000023 Rmu_sc0001493.1_g000081 Rmu_sc0001674.1_g000003 Rmu_sc0001791.1_g000023 Rmu_sc0001822.1_g000005 Rmu_sc0001822.1_g000033 Rmu_sc0002061.1_g000010 Rmu_sc0002286.1_g000012 Rmu_sc0002979.1_g000009 Rmu_sc0003158.1_g000016 Rmu_sc0003221.1_g000001 Rmu_sc0003641.1_g000029 Rmu_sc0003742.1_g000012 Rmu_sc0003814.1_g000004 Rmu_sc0003823.1_g000005 Rmu_sc0004002.1_g000005 Rmu_sc0004564.1_g000005 Rmu_sc0005415.1_g000003 Rmu_sc0006050.1_g000025 Rmu_sc0006555.1_g000002 Rmu_sc0009954.1_g000020 Rmu_sc0010421.1_g000007 Rmu_sc0010607.1_g000002 Rmu_sc0011044.1_g000005 Rmu_sc0011497.1_g000015 Rmu_sc0014532.1_g000004 Rmu_sc0014745.1_g000005 Rmu_sc0014821.1_g000001 Rmu_sc0015038.1_g000002 Rmu_sc0015454.1_g000004 Rmu_sc0019663.1_g000002 Rmu_sc0027475.1_g000003 Rmu_sc0028826.1_g000002 Rmu_ssc0000117.1_g000019 Rmu_ssc0000175.1_g000034
rosa_roxburghii Rroxscaffold_3G00237080 Rroxscaffold_4G00288290 Rroxscaffold_6G00397510
rosa_rugosa Rorug01G0035000 Rorug01G0190900 Rorug01G0208500 Rorug01G0208600 Rorug01G0208600 Rorug01G0344900 Rorug01G0479500 Rorug01G0479600 Rorug02G0230400 Rorug02G0320900 Rorug02G0433300 Rorug03G0147400 Rorug03G0169700.1 Rorug03G0169800 Rorug03G0198300 Rorug04G0022900 Rorug04G0064600 Rorug04G0322100 Rorug04G0388900 Rorug05G0153200 Rorug05G0231600 Rorug05G0231700 Rorug05G0231800 Rorug05G0276200 Rorug05G0292400 Rorug05G0314700 Rorug06G0092000 Rorug06G0260900 Rorug06G0273600 RorugPtG0003200
rosa_samantha Rh1AG264100 Rh1AG292500 Rh1AG423800 Rh2AG098000 Rh2AG232700 Rh2AG361100 Rh2AG372900 Rh2BG099200 Rh2BG246000 Rh2BG260600 Rh2BG378400 Rh3BG142300 Rh3BG252900 Rh3BG267700 Rh3DG302400 Rh5BG059800 Rh5BG320000 Rh5BG530500 Rh6AG089300 Rh6AG089400 Rh6AG127400 Rh6AG376700 Rh7CG488700 Rh7DG265900
rosa_wichuraiana Rw2G040640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1167
Acc36I ACCTGC 1 cut(s) 288
AccB7I CCANNNNNTGG 1 cut(s) 87
AccBSI CCGCTC 1 cut(s) 478
AccI GTMKAC 2 cut(s) 75, 915
AccIII TCCGGA 2 cut(s) 258, 1054
AciI CCGC 3 cut(s) 119, 370, 478
AclWI GGATC 2 cut(s) 149, 186
AcsI RAATTY 4 cut(s) 344, 358, 541, 1058
AfaI GTAC 2 cut(s) 943, 1116
AfiI CCNNNNNNNGG 7 cut(s) 87, 213, 265, 554, 555, 623, 782
AgsI TTSAA 4 cut(s) 52, 487, 967, 977
AjiI CACGTC 1 cut(s) 464
AjnI CCWGG 2 cut(s) 549, 775
AluBI AGCT 7 cut(s) 26, 104, 439, 642, 676, 843, 980
AluI AGCT 7 cut(s) 26, 104, 439, 642, 676, 843, 980
Alw26I GTCTC 3 cut(s) 713, 1002, 1023
AlwI GGATC 2 cut(s) 149, 186
Ama87I CYCGRG 1 cut(s) 69
Aor13HI TCCGGA 2 cut(s) 258, 1054
AoxI GGCC 2 cut(s) 515, 614
ApeKI GCWGC 4 cut(s) 245, 283, 286, 439
ApoI RAATTY 4 cut(s) 344, 358, 541, 1058
ArsI GACNNNNNNTTYG 2 cut(s) 868, 900
AspS9I GGNCC 2 cut(s) 277, 515
AsuC2I CCSGG 1 cut(s) 1137
AsuHPI GGTGA 2 cut(s) 1054, 1082
AsuII TTCGAA 1 cut(s) 354
AvaI CYCGRG 1 cut(s) 69
AvaII GGWCC 1 cut(s) 277
BaeGI GKGCMC 1 cut(s) 538
BbvI GCAGC 4 cut(s) 232, 270, 298, 426
BccI CCATC 6 cut(s) 268, 317, 629, 698, 806, 1009
BcgI CGANNNNNNTGC 2 cut(s) 720, 754
BciT130I CCWGG 2 cut(s) 551, 777
BcnI CCSGG 1 cut(s) 1137
BcoDI GTCTC 3 cut(s) 713, 1002, 1023
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 2 cut(s) 246, 284
BfuAI ACCTGC 1 cut(s) 288
BglI GCCNNNNNGGC 1 cut(s) 514
BisI GCNGC 5 cut(s) 119, 246, 284, 287, 440
BlsI GCNGC 5 cut(s) 120, 247, 285, 288, 441
Bme1390I CCNGG 3 cut(s) 551, 777, 1137
Bme18I GGWCC 1 cut(s) 277
BmeT110I CYCGRG 1 cut(s) 69
BmgBI CACGTC 1 cut(s) 464
BmgT120I GGNCC 2 cut(s) 277, 515
BmiI GGNNCC 1 cut(s) 1101
BmrFI CCNGG 3 cut(s) 551, 777, 1137
BmsI GCATC 1 cut(s) 859
BoxI GACNNNNGTC 1 cut(s) 71
BplI GAGNNNNNCTC 2 cut(s) 1003, 1035
BpmI CTGGAG 1 cut(s) 494
Bpu10I CCTNAGC 1 cut(s) 709
Bpu14I TTCGAA 1 cut(s) 354
BpuMI CCSGG 1 cut(s) 1137
BsaBI GATNNNNATC 1 cut(s) 183
BsaJI CCNNGG 2 cut(s) 549, 879
BsaWI WCCGGW 2 cut(s) 258, 1054
BsaXI ACNNNNNCTCC 2 cut(s) 436, 466
Bsc4I CCNNNNNNNGG 7 cut(s) 87, 213, 265, 554, 555, 623, 782
Bse118I RCCGGY 2 cut(s) 508, 901
Bse3DI GCAATG 2 cut(s) 771, 898
Bse8I GATNNNNATC 1 cut(s) 183
BseAI TCCGGA 2 cut(s) 258, 1054
BseBI CCWGG 2 cut(s) 551, 777
BseDI CCNNGG 2 cut(s) 549, 879
BseGI GGATG 3 cut(s) 811, 817, 874
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 7 cut(s) 87, 213, 265, 554, 555, 623, 782
BseMI GCAATG 2 cut(s) 771, 898
BseMII CTCAG 2 cut(s) 723, 897
BseSI GKGCMC 1 cut(s) 538
BseXI GCAGC 4 cut(s) 232, 270, 298, 426
BseYI CCCAGC 1 cut(s) 512
BsgI GTGCAG 2 cut(s) 242, 671
BshFI GGCC 2 cut(s) 517, 616
BsiHKCI CYCGRG 1 cut(s) 69
BsiSI CCGG 6 cut(s) 164, 259, 509, 902, 1055, 1136
BslFI GGGAC 1 cut(s) 1152
BslI CCNNNNNNNGG 7 cut(s) 87, 213, 265, 554, 555, 623, 782
BsmAI GTCTC 3 cut(s) 713, 1002, 1023
BsmFI GGGAC 1 cut(s) 1152
BsmI GAATGC 1 cut(s) 769
BsnI GGCC 2 cut(s) 517, 616
BsoBI CYCGRG 1 cut(s) 69
Bsp119I TTCGAA 1 cut(s) 354
Bsp1286I GDGCHC 1 cut(s) 538
Bsp13I TCCGGA 2 cut(s) 258, 1054
Bsp143I GATC 4 cut(s) 141, 178, 745, 1125
Bsp19I CCATGG 1 cut(s) 879
BspACI CCGC 3 cut(s) 119, 370, 478
BspANI GGCC 2 cut(s) 517, 616
BspCNI CTCAG 2 cut(s) 722, 898
BspEI TCCGGA 2 cut(s) 258, 1054
BspLI GGNNCC 1 cut(s) 1101
BspMAI CTGCAG 2 cut(s) 250, 288
BspMI ACCTGC 1 cut(s) 288
BspPI GGATC 2 cut(s) 149, 186
BspT104I TTCGAA 1 cut(s) 354
BsrBI CCGCTC 1 cut(s) 478
BsrDI GCAATG 2 cut(s) 771, 898
BsrFI RCCGGY 2 cut(s) 508, 901
BssAI RCCGGY 2 cut(s) 508, 901
BssECI CCNNGG 2 cut(s) 549, 879
BssMI GATC 4 cut(s) 141, 178, 745, 1125
BssNAI GTATAC 1 cut(s) 916
BssT1I CCWWGG 1 cut(s) 879
Bst1107I GTATAC 1 cut(s) 916
Bst2UI CCWGG 2 cut(s) 551, 777
Bst4CI ACNGT 3 cut(s) 316, 411, 580
Bst6I CTCTTC 2 cut(s) 917, 1004
BstBI TTCGAA 1 cut(s) 354
BstC8I GCNNGC 5 cut(s) 432, 510, 678, 733, 903
BstDEI CTNAG 4 cut(s) 188, 696, 709, 906
BstDSI CCRYGG 1 cut(s) 879
BstF5I GGATG 3 cut(s) 811, 817, 874
BstKTI GATC 4 cut(s) 144, 181, 748, 1128
BstMAI GTCTC 3 cut(s) 713, 1002, 1023
BstMBI GATC 4 cut(s) 141, 178, 745, 1125
BstMWI GCNNNNNNNGC 3 cut(s) 436, 514, 796
BstNI CCWGG 2 cut(s) 551, 777
BstPAI GACNNNNGTC 1 cut(s) 71
BstSCI CCNGG 3 cut(s) 549, 775, 1135
BstSFI CTRYAG 2 cut(s) 246, 284
BstSLI GKGCMC 1 cut(s) 538
BstV1I GCAGC 4 cut(s) 232, 270, 298, 426
BstZ17I GTATAC 1 cut(s) 916
BsuRI GGCC 2 cut(s) 517, 616
BtgI CCRYGG 1 cut(s) 879
BtrI CACGTC 1 cut(s) 464
BtsCI GGATG 3 cut(s) 811, 817, 874
BtsI GCAGTG 1 cut(s) 634
BtsIMutI CAGTG 1 cut(s) 634
BveI ACCTGC 1 cut(s) 288
Cac8I GCNNGC 5 cut(s) 432, 510, 678, 733, 903
Cfr10I RCCGGY 2 cut(s) 508, 901
Cfr13I GGNCC 2 cut(s) 277, 515
CseI GACGC 1 cut(s) 779
Csp6I GTAC 2 cut(s) 942, 1115
CspCI CAANNNNNGTGG 8 cut(s) 133, 168, 223, 258, 364, 399, 651, 686
CviAII CATG 3 cut(s) 398, 749, 880
CviQI GTAC 2 cut(s) 942, 1115
DdeI CTNAG 4 cut(s) 188, 696, 709, 906
DpnI GATC 4 cut(s) 143, 180, 747, 1127
DpnII GATC 4 cut(s) 141, 178, 745, 1125
Eam1104I CTCTTC 2 cut(s) 917, 1004
EarI CTCTTC 2 cut(s) 917, 1004
Eco130I CCWWGG 1 cut(s) 879
Eco147I AGGCCT 1 cut(s) 616
Eco47I GGWCC 1 cut(s) 277
Eco88I CYCGRG 1 cut(s) 69
EcoRI GAATTC 2 cut(s) 344, 541
EcoRII CCWGG 2 cut(s) 549, 775
EcoT14I CCWWGG 1 cut(s) 879
EcoT22I ATGCAT 1 cut(s) 771
ErhI CCWWGG 1 cut(s) 879
FaeI CATG 3 cut(s) 401, 752, 883
FalI AAGNNNNNCTT 2 cut(s) 591, 623
FaqI GGGAC 1 cut(s) 1152
FatI CATG 3 cut(s) 397, 748, 879
FauI CCCGC 1 cut(s) 471
FblI GTMKAC 2 cut(s) 75, 915
Fnu4HI GCNGC 5 cut(s) 119, 246, 284, 287, 440
FokI GGATG 3 cut(s) 818, 824, 881
Fsp4HI GCNGC 5 cut(s) 119, 246, 284, 287, 440
FspBI CTAG 1 cut(s) 23
GluI GCNGC 5 cut(s) 119, 246, 284, 287, 440
GsaI CCCAGC 1 cut(s) 516
GsuI CTGGAG 1 cut(s) 494
HaeIII GGCC 2 cut(s) 517, 616
HapII CCGG 6 cut(s) 164, 259, 509, 902, 1055, 1136
HgaI GACGC 1 cut(s) 779
Hin1II CATG 3 cut(s) 401, 752, 883
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HindIII AAGCTT 1 cut(s) 978
HinfI GANTC 7 cut(s) 67, 72, 77, 262, 309, 659, 757
HpaII CCGG 6 cut(s) 164, 259, 509, 902, 1055, 1136
HphI GGTGA 2 cut(s) 1054, 1082
Hpy166II GTNNAC 6 cut(s) 76, 155, 386, 692, 785, 916
Hpy188I TCNGA 3 cut(s) 418, 497, 703
Hpy188III TCNNGA 6 cut(s) 139, 259, 473, 866, 1055, 1123
Hpy8I GTNNAC 6 cut(s) 76, 155, 386, 692, 785, 916
Hpy99I CGWCG 1 cut(s) 795
HpyAV CCTTC 4 cut(s) 220, 627, 904, 1090
HpyCH4III ACNGT 3 cut(s) 316, 411, 580
HpyCH4IV ACGT 1 cut(s) 463
HpyF10VI GCNNNNNNNGC 3 cut(s) 436, 514, 796
HpyF3I CTNAG 4 cut(s) 188, 696, 709, 906
HpySE526I ACGT 1 cut(s) 463
Hsp92II CATG 3 cut(s) 401, 752, 883
Kpn2I TCCGGA 2 cut(s) 258, 1054
KroI GCCGGC 2 cut(s) 508, 901
KroNI GCCGGC 2 cut(s) 510, 903
Kzo9I GATC 4 cut(s) 141, 178, 745, 1125
LmnI GCTCC 4 cut(s) 101, 475, 673, 728
Lsp1109I GCAGC 4 cut(s) 232, 270, 298, 426
LweI GCATC 1 cut(s) 859
MaeI CTAG 1 cut(s) 23
MaeII ACGT 1 cut(s) 463
MaeIII GTNAC 2 cut(s) 7, 391
MalI GATC 4 cut(s) 143, 180, 747, 1127
MbiI CCGCTC 1 cut(s) 478
MboI GATC 4 cut(s) 141, 178, 745, 1125
MboII GAAGA 6 cut(s) 340, 343, 867, 934, 1021, 1101
MhlI GDGCHC 1 cut(s) 538
MluCI AATT 4 cut(s) 344, 358, 541, 1058
MlyI GAGTC 4 cut(s) 61, 71, 81, 653
MmeI TCCRAC 3 cut(s) 79, 475, 832
MnlI CCTC 8 cut(s) 186, 558, 588, 606, 661, 802, 920, 1066
Mph1103I ATGCAT 1 cut(s) 771
MroI TCCGGA 2 cut(s) 258, 1054
MroNI GCCGGC 2 cut(s) 508, 901
MseI TTAA 4 cut(s) 38, 92, 123, 582
MspI CCGG 6 cut(s) 164, 259, 509, 902, 1055, 1136
MspR9I CCNGG 3 cut(s) 551, 777, 1137
Mva1269I GAATGC 1 cut(s) 769
MvaI CCWGG 2 cut(s) 551, 777
MwoI GCNNNNNNNGC 3 cut(s) 436, 514, 796
NaeI GCCGGC 2 cut(s) 510, 903
NciI CCSGG 1 cut(s) 1137
NcoI CCATGG 1 cut(s) 879
NdeII GATC 4 cut(s) 141, 178, 745, 1125
NgoMIV GCCGGC 2 cut(s) 508, 901
NlaIII CATG 3 cut(s) 401, 752, 883
NlaIV GGNNCC 1 cut(s) 1101
NmuCI GTSAC 2 cut(s) 7, 391
NsiI ATGCAT 1 cut(s) 771
NspV TTCGAA 1 cut(s) 354
PaeR7I CTCGAG 1 cut(s) 69
PceI AGGCCT 1 cut(s) 616
PcsI WCGNNNNNNNCGW 1 cut(s) 787
PctI GAATGC 1 cut(s) 769
PdiI GCCGGC 2 cut(s) 510, 903
PfeI GAWTC 3 cut(s) 262, 309, 757
PflFI GACNNNGTC 1 cut(s) 789
PflMI CCANNNNNTGG 1 cut(s) 87
PkrI GCNGC 5 cut(s) 120, 247, 285, 288, 441
PleI GAGTC 4 cut(s) 61, 71, 80, 653
PpsI GAGTC 4 cut(s) 61, 71, 80, 653
PshAI GACNNNNGTC 1 cut(s) 71
PsiI TTATAA 1 cut(s) 1167
Psp6I CCWGG 2 cut(s) 549, 775
PspFI CCCAGC 1 cut(s) 512
PspGI CCWGG 2 cut(s) 549, 775
PspN4I GGNNCC 1 cut(s) 1101
PspPI GGNCC 2 cut(s) 277, 515
PspXI VCTCGAGB 1 cut(s) 69
PstI CTGCAG 2 cut(s) 250, 288
PsyI GACNNNGTC 1 cut(s) 789
RsaI GTAC 2 cut(s) 943, 1116
RsaNI GTAC 2 cut(s) 942, 1115
SalI GTCGAC 1 cut(s) 74
SaqAI TTAA 4 cut(s) 38, 92, 123, 582
SatI GCNGC 5 cut(s) 119, 246, 284, 287, 440
Sau3AI GATC 4 cut(s) 141, 178, 745, 1125
Sau96I GGNCC 2 cut(s) 277, 515
SchI GAGTC 4 cut(s) 61, 71, 81, 653
ScrFI CCNGG 3 cut(s) 551, 777, 1137
SduI GDGCHC 1 cut(s) 538
SfaNI GCATC 1 cut(s) 859
SfcI CTRYAG 2 cut(s) 246, 284
Sfr274I CTCGAG 1 cut(s) 69
SfuI TTCGAA 1 cut(s) 354
SinI GGWCC 1 cut(s) 277
SlaI CTCGAG 1 cut(s) 69
SmlI CTYRAG 1 cut(s) 69
SmoI CTYRAG 1 cut(s) 69
Sse9I AATT 4 cut(s) 344, 358, 541, 1058
SseBI AGGCCT 1 cut(s) 616
SsiI CCGC 3 cut(s) 119, 370, 478
SspMI CTAG 1 cut(s) 23
StuI AGGCCT 1 cut(s) 616
StyD4I CCNGG 3 cut(s) 549, 775, 1135
StyI CCWWGG 1 cut(s) 879
TaaI ACNGT 3 cut(s) 316, 411, 580
TaiI ACGT 1 cut(s) 466
TaqI TCGA 5 cut(s) 70, 75, 354, 740, 935
TasI AATT 4 cut(s) 344, 358, 541, 1058
TatI WGTACW 1 cut(s) 1114
TauI GCSGC 1 cut(s) 121
TfiI GAWTC 3 cut(s) 262, 309, 757
Tru1I TTAA 4 cut(s) 38, 92, 123, 582
Tru9I TTAA 4 cut(s) 38, 92, 123, 582
TscAI CASTG 1 cut(s) 634
TseFI GTSAC 2 cut(s) 7, 391
TseI GCWGC 4 cut(s) 245, 283, 286, 439
Tsp45I GTSAC 2 cut(s) 7, 391
TspDTI ATGAA 4 cut(s) 74, 161, 1004, 1146
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 1 cut(s) 634
Tth111I GACNNNGTC 1 cut(s) 789
Van91I CCANNNNNTGG 1 cut(s) 87
VpaK11BI GGWCC 1 cut(s) 277
XapI RAATTY 4 cut(s) 344, 358, 541, 1058
XhoI CTCGAG 1 cut(s) 69
XmiI GTMKAC 2 cut(s) 75, 915
XspI CTAG 1 cut(s) 23
Zsp2I ATGCAT 1 cut(s) 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.