Rorug03G0147400

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000336
Physical Location & Seq
Forward (+)
12119865 .. 12121100
1236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0147400.1

Sequence Viewer

Length: 882 bp
ATGGCTGTTAAAACTCTTGTACTTGTTTATGCTATGAAGACCATTGATGAGCTGAAATACAAGCTTCTCAGCATCAACTATGAACTAGAATCAGCGCAAATAAGGGCGAATCAGGAGGTAAGCAAAAACGAGGAGATCACAAAGCAGTTGTTTCATCTCTTGAAGGTAGCTTGTCGAGAAAGAGATGAAGCAAAAGACCAGTTGCAACAACTACTCAAAATGTTCTTGCCCATACCAGCTGAAAATGAAACATGCCCTATGTTCTCTGAGGTCAACAATGAAAATTATCCAAGTCTGCAAAAAGCCTCAAATTTCTCAACAGTTAAAGAATCAGATGTTATATCCAAAACTAGTTCATATCCCACTAAATACAACTTTGCTGCTGATTCAAGTAACTTAGGATTTTCCAAGAATGCTGCTGTTCAAGGGTGTGATGAGTCCGTTCAGGTGGCTACGATGTCTTTATCGAACTCAAAGATCATTGATCGTGCTGATGTTTTGATTGATAATATTGTTAGGGTCAGACCACTGCCTAAGAAAGGAAGATTGTTCCAGGCTGTAATGGAAAATGAGCCTTTACTTCAGACACTTCTTGTTGCTCCTCTTCCCAAGTGGAAAAACCCTCCACCTCTTCCTCCTTTAAAAAGTTCATCCAATCTCATTTATGGATGTGACTTTGGCTCACAAGAACAGATTGAAGCAGTCAAGCCAATTCGTGCAGTACAAGGTTCAAGCTCACTCGGATTTTCTCACATACCATCTTCATCTGTGTTGGACTTTGAACATGGTTCTGCTAGTGTGAAGACTCGAGTTGGTAACAGTTCAAGTGATCTCATGCAGAGTCAGATTGGGACTTGTAAGAGAAGAAAAAGAAGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

293

Amino Acids

32.65

Weight (kDa)

8.96

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1635 PF07795 13 - 211 1e-30 Protein of unknown function (DUF1635)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000336)

Species Orthologous Gene IDs
malus_domestica MD05G1024100.v1.1
rosa_chinensis RchiOBHm_Chr2g0152291 RchiOBHm_Chr4g0387411
rosa_laevigata RLG00000009698 RLG00000009699 RLG00000009700 RLG00000023615 RLG00000024265 RLG00000034445 RLG00000036567
rosa_multiflora Rmu_co8268067.1_g000001 Rmu_co8313555.1_g000001 Rmu_co8324697.1_g000001 Rmu_sc0000318.1_g000023 Rmu_sc0000342.1_g000016 Rmu_sc0000447.1_g000010 Rmu_sc0000528.1_g000009 Rmu_sc0000539.1_g000060 Rmu_sc0000929.1_g000023 Rmu_sc0001493.1_g000081 Rmu_sc0001674.1_g000003 Rmu_sc0001791.1_g000023 Rmu_sc0001822.1_g000005 Rmu_sc0001822.1_g000033 Rmu_sc0002061.1_g000010 Rmu_sc0002286.1_g000012 Rmu_sc0002979.1_g000009 Rmu_sc0003158.1_g000016 Rmu_sc0003221.1_g000001 Rmu_sc0003641.1_g000029 Rmu_sc0003742.1_g000012 Rmu_sc0003814.1_g000004 Rmu_sc0003823.1_g000005 Rmu_sc0004002.1_g000005 Rmu_sc0004564.1_g000005 Rmu_sc0005415.1_g000003 Rmu_sc0006050.1_g000025 Rmu_sc0006555.1_g000002 Rmu_sc0009954.1_g000020 Rmu_sc0010421.1_g000007 Rmu_sc0010607.1_g000002 Rmu_sc0011044.1_g000005 Rmu_sc0011497.1_g000015 Rmu_sc0014532.1_g000004 Rmu_sc0014745.1_g000005 Rmu_sc0014821.1_g000001 Rmu_sc0015038.1_g000002 Rmu_sc0015454.1_g000004 Rmu_sc0019663.1_g000002 Rmu_sc0027475.1_g000003 Rmu_sc0028826.1_g000002 Rmu_ssc0000117.1_g000019 Rmu_ssc0000175.1_g000034
rosa_roxburghii Rroxscaffold_3G00237080 Rroxscaffold_4G00288290 Rroxscaffold_6G00397510
rosa_rugosa Rorug01G0035000 Rorug01G0190900 Rorug01G0208500 Rorug01G0208600 Rorug01G0208600 Rorug01G0344900 Rorug01G0479500 Rorug01G0479600 Rorug02G0230400 Rorug02G0320900 Rorug02G0433300 Rorug03G0147400 Rorug03G0169700.1 Rorug03G0169800 Rorug03G0198300 Rorug04G0022900 Rorug04G0064600 Rorug04G0322100 Rorug04G0388900 Rorug05G0153200 Rorug05G0231600 Rorug05G0231700 Rorug05G0231800 Rorug05G0276200 Rorug05G0292400 Rorug05G0314700 Rorug06G0092000 Rorug06G0260900 Rorug06G0273600 RorugPtG0003200
rosa_samantha Rh1AG264100 Rh1AG292500 Rh1AG423800 Rh2AG098000 Rh2AG232700 Rh2AG361100 Rh2AG372900 Rh2BG099200 Rh2BG246000 Rh2BG260600 Rh2BG378400 Rh3BG142300 Rh3BG252900 Rh3BG267700 Rh3DG302400 Rh5BG059800 Rh5BG320000 Rh5BG530500 Rh6AG089300 Rh6AG089400 Rh6AG127400 Rh6AG376700 Rh7CG488700 Rh7DG265900
rosa_wichuraiana Rw2G040640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 310
AcuI CTGAAG 1 cut(s) 566
AfaI GTAC 2 cut(s) 21, 723
AfiI CCNNNNNNNGG 1 cut(s) 539
AgsI TTSAA 7 cut(s) 163, 390, 425, 698, 732, 782, 825
AhlI ACTAGT 1 cut(s) 350
AjnI CCWGG 1 cut(s) 552
AluBI AGCT 5 cut(s) 52, 64, 170, 239, 735
AluI AGCT 5 cut(s) 52, 64, 170, 239, 735
Ama87I CYCGRG 1 cut(s) 807
ApeKI GCWGC 2 cut(s) 380, 416
ApoI RAATTY 1 cut(s) 310
AspLEI GCGC 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 807
BbsI GAAGAC 2 cut(s) 44, 809
BbvI GCAGC 2 cut(s) 367, 403
BccI CCATC 1 cut(s) 766
BciT130I CCWGG 1 cut(s) 554
BcuI ACTAGT 1 cut(s) 350
BfaI CTAG 3 cut(s) 86, 351, 795
BisI GCNGC 2 cut(s) 381, 417
BlsI GCNGC 2 cut(s) 382, 418
Bme1390I CCNGG 1 cut(s) 554
BmeT110I CYCGRG 1 cut(s) 807
BmrFI CCNGG 1 cut(s) 554
BmsI GCATC 1 cut(s) 81
BpiI GAAGAC 2 cut(s) 44, 809
Bsc4I CCNNNNNNNGG 1 cut(s) 539
Bse1I ACTGG 1 cut(s) 199
BseBI CCWGG 1 cut(s) 554
BseGI GGATG 2 cut(s) 650, 674
BseLI CCNNNNNNNGG 1 cut(s) 539
BseMII CTCAG 2 cut(s) 82, 258
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 2 cut(s) 146, 591
BseXI GCAGC 2 cut(s) 367, 403
BsgI GTGCAG 1 cut(s) 738
BsiHKCI CYCGRG 1 cut(s) 807
BslFI GGGAC 1 cut(s) 865
BslI CCNNNNNNNGG 1 cut(s) 539
BsmFI GGGAC 1 cut(s) 865
BsmI GAATGC 1 cut(s) 418
BsoBI CYCGRG 1 cut(s) 807
Bsp143I GATC 4 cut(s) 135, 477, 484, 829
BspCNI CTCAG 2 cut(s) 81, 259
BsrI ACTGG 1 cut(s) 199
BssMI GATC 4 cut(s) 135, 477, 484, 829
Bst2UI CCWGG 1 cut(s) 554
Bst4CI ACNGT 2 cut(s) 322, 821
Bst6I CTCTTC 2 cut(s) 609, 636
BstDEI CTNAG 4 cut(s) 68, 267, 397, 534
BstENI CCTNNNNNAGG 1 cut(s) 537
BstF5I GGATG 2 cut(s) 650, 674
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 4 cut(s) 138, 480, 487, 832
BstMBI GATC 4 cut(s) 135, 477, 484, 829
BstNI CCWGG 1 cut(s) 554
BstNSI RCATGY 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 552
BstV1I GCAGC 2 cut(s) 367, 403
BstV2I GAAGAC 2 cut(s) 44, 809
BtsCI GGATG 2 cut(s) 650, 674
BtsI GCAGTG 1 cut(s) 527
BtsIMutI CAGTG 1 cut(s) 527
CfoI GCGC 1 cut(s) 97
Csp6I GTAC 2 cut(s) 20, 722
CviAII CATG 3 cut(s) 252, 785, 835
CviQI GTAC 2 cut(s) 20, 722
DdeI CTNAG 4 cut(s) 68, 267, 397, 534
DpnI GATC 4 cut(s) 137, 479, 486, 831
DpnII GATC 4 cut(s) 135, 477, 484, 829
DraI TTTAAA 1 cut(s) 642
Eam1104I CTCTTC 2 cut(s) 609, 636
EarI CTCTTC 2 cut(s) 609, 636
Eco57I CTGAAG 1 cut(s) 566
Eco88I CYCGRG 1 cut(s) 807
EcoNI CCTNNNNNAGG 1 cut(s) 537
EcoRII CCWGG 1 cut(s) 552
FaeI CATG 3 cut(s) 255, 788, 838
FaqI GGGAC 1 cut(s) 865
FatI CATG 3 cut(s) 251, 784, 834
Fnu4HI GCNGC 2 cut(s) 381, 417
FokI GGATG 2 cut(s) 637, 681
Fsp4HI GCNGC 2 cut(s) 381, 417
FspBI CTAG 3 cut(s) 86, 351, 795
GlaI GCGC 1 cut(s) 96
GluI GCNGC 2 cut(s) 381, 417
HhaI GCGC 1 cut(s) 97
Hin1II CATG 3 cut(s) 255, 788, 838
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 7 cut(s) 89, 109, 329, 386, 437, 805, 841
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 7 cut(s) 268, 334, 524, 585, 743, 846, 881
Hpy188III TCNNGA 3 cut(s) 113, 160, 176
Hpy8I GTNNAC 1 cut(s) 274
HpyAV CCTTC 2 cut(s) 157, 867
HpyCH4III ACNGT 2 cut(s) 322, 821
HpyCH4V TGCA 4 cut(s) 205, 298, 719, 838
HpyF3I CTNAG 4 cut(s) 68, 267, 397, 534
Hsp92II CATG 3 cut(s) 255, 788, 838
HspAI GCGC 1 cut(s) 95
Kzo9I GATC 4 cut(s) 135, 477, 484, 829
LmnI GCTCC 1 cut(s) 604
LpnPI CCDG 6 cut(s) 98, 212, 249, 431, 539, 566
Lsp1109I GCAGC 2 cut(s) 367, 403
LweI GCATC 1 cut(s) 81
MaeI CTAG 3 cut(s) 86, 351, 795
MaeIII GTNAC 3 cut(s) 392, 671, 815
MalI GATC 4 cut(s) 137, 479, 486, 831
MboI GATC 4 cut(s) 135, 477, 484, 829
MboII GAAGA 7 cut(s) 49, 555, 596, 623, 753, 814, 876
MluCI AATT 3 cut(s) 283, 310, 711
MlyI GAGTC 3 cut(s) 446, 799, 850
MmeI TCCRAC 1 cut(s) 753
MnlI CCTC 8 cut(s) 109, 124, 262, 316, 612, 633, 639, 645
MseI TTAA 3 cut(s) 9, 324, 641
MspA1I CMGCKG 1 cut(s) 239
MspR9I CCNGG 1 cut(s) 554
Mva1269I GAATGC 1 cut(s) 418
MvaI CCWGG 1 cut(s) 554
NdeII GATC 4 cut(s) 135, 477, 484, 829
NlaIII CATG 3 cut(s) 255, 788, 838
NmuCI GTSAC 1 cut(s) 671
NspI RCATGY 1 cut(s) 255
PaeR7I CTCGAG 1 cut(s) 807
PctI GAATGC 1 cut(s) 418
PfeI GAWTC 4 cut(s) 89, 109, 329, 386
PkrI GCNGC 2 cut(s) 382, 418
PleI GAGTC 3 cut(s) 445, 799, 849
PpsI GAGTC 3 cut(s) 445, 799, 849
Psp6I CCWGG 1 cut(s) 552
PspGI CCWGG 1 cut(s) 552
PspXI VCTCGAGB 1 cut(s) 807
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 2 cut(s) 21, 723
RsaNI GTAC 2 cut(s) 20, 722
SaqAI TTAA 3 cut(s) 9, 324, 641
SatI GCNGC 2 cut(s) 381, 417
Sau3AI GATC 4 cut(s) 135, 477, 484, 829
SchI GAGTC 3 cut(s) 446, 799, 850
ScrFI CCNGG 1 cut(s) 554
SfaNI GCATC 1 cut(s) 81
Sfr274I CTCGAG 1 cut(s) 807
SlaI CTCGAG 1 cut(s) 807
SmlI CTYRAG 1 cut(s) 807
SmoI CTYRAG 1 cut(s) 807
SpeI ACTAGT 1 cut(s) 350
Sse9I AATT 3 cut(s) 283, 310, 711
SspI AATATT 1 cut(s) 511
SspMI CTAG 3 cut(s) 86, 351, 795
StyD4I CCNGG 1 cut(s) 552
TaaI ACNGT 2 cut(s) 322, 821
TaqI TCGA 3 cut(s) 175, 467, 808
TasI AATT 3 cut(s) 283, 310, 711
TatI WGTACW 2 cut(s) 19, 721
TfiI GAWTC 4 cut(s) 89, 109, 329, 386
Tru1I TTAA 3 cut(s) 9, 324, 641
Tru9I TTAA 3 cut(s) 9, 324, 641
TscAI CASTG 1 cut(s) 534
TseFI GTSAC 1 cut(s) 671
TseI GCWGC 2 cut(s) 380, 416
Tsp45I GTSAC 1 cut(s) 671
TspDTI ATGAA 9 cut(s) 50, 96, 143, 201, 261, 294, 345, 639, 753
TspGWI ACGGA 1 cut(s) 430
TspRI CASTG 1 cut(s) 534
XagI CCTNNNNNAGG 1 cut(s) 537
XapI RAATTY 1 cut(s) 310
XceI RCATGY 1 cut(s) 255
XhoI CTCGAG 1 cut(s) 807
XspI CTAG 3 cut(s) 86, 351, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.