Rh3BG252900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000336
Physical Location & Seq
Forward (+)
24459076 .. 24467622
8547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG252900.1

Sequence Viewer

Length: 669 bp
ATGAACTTTGGTGGATGCATGTTGAAAGGGTCCGTACCTGTAGCTGATGCTGCATATGAATGTCCGCTGTTAGGGGAGGCCCTAAGGGAGGATACTTCGCCCGTTCAGCGCAACTTGGAACGAGTGCTGGCTCGGGAAGACACCACTCCGGCCTCTGGACCAGCATTAGTCTTTAGGGCACGAGTGGAAGAGGAAGTTGTTGCAGGTCTTGATGGTAGCAGCAAGGCTACACGGGTGGTGGAGAGCCCTGTGGTGGGGGTGGAGTCCAGTGGTGGTAATGGCAGTAATGAATTGTCTTCTAGAGATGTGTTGCTCGATCAGAGTATGGCACAGTTGGTTGGTATGAGGAGGAAACGTGTTGAGAACCTGGGAGTTTCCCCCAAGAAGCCTCGATTTAGTCTTGCAGTTGGGAGAACCAACCTTGATAATGTCTCTATAGGGTTGGTTGTGATGAATGCTGCTCAAGAAGGCACTAAGAAACAAGGCCGTCCTCGTGGTGCCAAGAATAAGACCAAGGCAAACTACAAAGTGGTCAAGACTTCACCCATGAAGCGTAGCAAAGGTAGCAAGAAAGGAGAACAGCTGGCTAATGGCCTTCCCGAGTTCTCTATGCCAGGAAAAGTTAGTACTCCTAAATCTACTAAGGGGAAGGAGGTAGTTTCTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

23.52

Weight (kDa)

9.93

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000336)

Species Orthologous Gene IDs
malus_domestica MD05G1024100.v1.1
rosa_chinensis RchiOBHm_Chr2g0152291 RchiOBHm_Chr4g0387411
rosa_laevigata RLG00000009698 RLG00000009699 RLG00000009700 RLG00000023615 RLG00000024265 RLG00000034445 RLG00000036567
rosa_multiflora Rmu_co8268067.1_g000001 Rmu_co8313555.1_g000001 Rmu_co8324697.1_g000001 Rmu_sc0000318.1_g000023 Rmu_sc0000342.1_g000016 Rmu_sc0000447.1_g000010 Rmu_sc0000528.1_g000009 Rmu_sc0000539.1_g000060 Rmu_sc0000929.1_g000023 Rmu_sc0001493.1_g000081 Rmu_sc0001674.1_g000003 Rmu_sc0001791.1_g000023 Rmu_sc0001822.1_g000005 Rmu_sc0001822.1_g000033 Rmu_sc0002061.1_g000010 Rmu_sc0002286.1_g000012 Rmu_sc0002979.1_g000009 Rmu_sc0003158.1_g000016 Rmu_sc0003221.1_g000001 Rmu_sc0003641.1_g000029 Rmu_sc0003742.1_g000012 Rmu_sc0003814.1_g000004 Rmu_sc0003823.1_g000005 Rmu_sc0004002.1_g000005 Rmu_sc0004564.1_g000005 Rmu_sc0005415.1_g000003 Rmu_sc0006050.1_g000025 Rmu_sc0006555.1_g000002 Rmu_sc0009954.1_g000020 Rmu_sc0010421.1_g000007 Rmu_sc0010607.1_g000002 Rmu_sc0011044.1_g000005 Rmu_sc0011497.1_g000015 Rmu_sc0014532.1_g000004 Rmu_sc0014745.1_g000005 Rmu_sc0014821.1_g000001 Rmu_sc0015038.1_g000002 Rmu_sc0015454.1_g000004 Rmu_sc0019663.1_g000002 Rmu_sc0027475.1_g000003 Rmu_sc0028826.1_g000002 Rmu_ssc0000117.1_g000019 Rmu_ssc0000175.1_g000034
rosa_roxburghii Rroxscaffold_3G00237080 Rroxscaffold_4G00288290 Rroxscaffold_6G00397510
rosa_rugosa Rorug01G0035000 Rorug01G0190900 Rorug01G0208500 Rorug01G0208600 Rorug01G0208600 Rorug01G0344900 Rorug01G0479500 Rorug01G0479600 Rorug02G0230400 Rorug02G0320900 Rorug02G0433300 Rorug03G0147400 Rorug03G0169700.1 Rorug03G0169800 Rorug03G0198300 Rorug04G0022900 Rorug04G0064600 Rorug04G0322100 Rorug04G0388900 Rorug05G0153200 Rorug05G0231600 Rorug05G0231700 Rorug05G0231800 Rorug05G0276200 Rorug05G0292400 Rorug05G0314700 Rorug06G0092000 Rorug06G0260900 Rorug06G0273600 RorugPtG0003200
rosa_samantha Rh1AG264100 Rh1AG292500 Rh1AG423800 Rh2AG098000 Rh2AG232700 Rh2AG361100 Rh2AG372900 Rh2BG099200 Rh2BG246000 Rh2BG260600 Rh2BG378400 Rh3BG142300 Rh3BG252900 Rh3BG267700 Rh3DG302400 Rh5BG059800 Rh5BG320000 Rh5BG530500 Rh6AG089300 Rh6AG089400 Rh6AG127400 Rh6AG376700 Rh7CG488700 Rh7DG265900
rosa_wichuraiana Rw2G040640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 194
AccB1I GGYRCC 1 cut(s) 497
AciI CCGC 1 cut(s) 65
AfaI GTAC 2 cut(s) 36, 628
AfiI CCNNNNNNNGG 5 cut(s) 71, 88, 155, 253, 254
AflIII ACRYGT 1 cut(s) 355
AgsI TTSAA 1 cut(s) 25
AjnI CCWGG 2 cut(s) 366, 613
AluBI AGCT 2 cut(s) 44, 583
AluI AGCT 2 cut(s) 44, 583
Alw26I GTCTC 1 cut(s) 436
Ama87I CYCGRG 2 cut(s) 132, 599
AoxI GGCC 4 cut(s) 78, 150, 484, 592
ApeKI GCWGC 3 cut(s) 50, 219, 458
AspLEI GCGC 1 cut(s) 111
AspS9I GGNCC 3 cut(s) 30, 79, 158
AsuHPI GGTGA 1 cut(s) 534
AvaI CYCGRG 2 cut(s) 132, 599
AvaII GGWCC 2 cut(s) 30, 158
AxyI CCTNAGG 1 cut(s) 83
BaeGI GKGCMC 1 cut(s) 181
BanI GGYRCC 1 cut(s) 497
BanII GRGCYC 1 cut(s) 248
BauI CACGAG 2 cut(s) 180, 492
BbsI GAAGAC 2 cut(s) 144, 288
BbvI GCAGC 3 cut(s) 37, 231, 445
BccI CCATC 1 cut(s) 206
BceAI ACGGC 1 cut(s) 471
BciT130I CCWGG 2 cut(s) 368, 615
BciVI GTATCC 1 cut(s) 85
BcoDI GTCTC 1 cut(s) 436
BfaI CTAG 2 cut(s) 300, 667
BfmI CTRYAG 2 cut(s) 39, 435
BfuAI ACCTGC 1 cut(s) 194
BfuI GTATCC 1 cut(s) 85
BisI GCNGC 3 cut(s) 51, 220, 459
BlsI GCNGC 3 cut(s) 52, 221, 460
BmcAI AGTACT 1 cut(s) 628
Bme1390I CCNGG 2 cut(s) 368, 615
Bme18I GGWCC 2 cut(s) 30, 158
BmeT110I CYCGRG 2 cut(s) 132, 599
BmgT120I GGNCC 3 cut(s) 30, 79, 158
BmiI GGNNCC 2 cut(s) 31, 499
BmrFI CCNGG 2 cut(s) 368, 615
BmsI GCATC 2 cut(s) 5, 37
BpiI GAAGAC 2 cut(s) 144, 288
BpuEI CTTGAG 1 cut(s) 447
BsaJI CCNNGG 2 cut(s) 367, 513
BsaXI ACNNNNNCTCC 2 cut(s) 340, 370
Bsc4I CCNNNNNNNGG 5 cut(s) 71, 88, 155, 253, 254
Bse1I ACTGG 1 cut(s) 267
Bse21I CCTNAGG 1 cut(s) 83
BseBI CCWGG 2 cut(s) 368, 615
BseDI CCNNGG 2 cut(s) 367, 513
BseGI GGATG 1 cut(s) 20
BseLI CCNNNNNNNGG 5 cut(s) 71, 88, 155, 253, 254
BseNI ACTGG 1 cut(s) 267
BseRI GAGGAG 1 cut(s) 361
BseSI GKGCMC 1 cut(s) 181
BseXI GCAGC 3 cut(s) 37, 231, 445
BshFI GGCC 4 cut(s) 80, 152, 486, 594
BshNI GGYRCC 1 cut(s) 497
BsiHKCI CYCGRG 2 cut(s) 132, 599
BsiSI CCGG 1 cut(s) 149
BslI CCNNNNNNNGG 5 cut(s) 71, 88, 155, 253, 254
BsmAI GTCTC 1 cut(s) 436
BsmI GAATGC 1 cut(s) 460
BsnI GGCC 4 cut(s) 80, 152, 486, 594
BsoBI CYCGRG 2 cut(s) 132, 599
Bsp1286I GDGCHC 2 cut(s) 181, 248
Bsp143I GATC 1 cut(s) 316
BspACI CCGC 1 cut(s) 65
BspANI GGCC 4 cut(s) 80, 152, 486, 594
BspLI GGNNCC 2 cut(s) 31, 499
BspMI ACCTGC 1 cut(s) 194
BspT107I GGYRCC 1 cut(s) 497
BsrI ACTGG 1 cut(s) 267
BssECI CCNNGG 2 cut(s) 367, 513
BssMI GATC 1 cut(s) 316
BssSI CACGAG 2 cut(s) 180, 492
BssT1I CCWWGG 1 cut(s) 513
Bst2BI CACGAG 2 cut(s) 180, 492
Bst2UI CCWGG 2 cut(s) 368, 615
Bst4CI ACNGT 1 cut(s) 333
Bst6I CTCTTC 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 129, 585
BstDEI CTNAG 3 cut(s) 83, 474, 642
BstENI CCTNNNNNAGG 1 cut(s) 86
BstF5I GGATG 1 cut(s) 20
BstHHI GCGC 1 cut(s) 111
BstKTI GATC 1 cut(s) 319
BstMAI GTCTC 1 cut(s) 436
BstMBI GATC 1 cut(s) 316
BstMWI GCNNNNNNNGC 3 cut(s) 50, 106, 564
BstNI CCWGG 2 cut(s) 368, 615
BstNSI RCATGY 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 366, 613
BstSFI CTRYAG 2 cut(s) 39, 435
BstSLI GKGCMC 1 cut(s) 181
BstV1I GCAGC 3 cut(s) 37, 231, 445
BstV2I GAAGAC 2 cut(s) 144, 288
Bsu36I CCTNAGG 1 cut(s) 83
BsuI GTATCC 1 cut(s) 85
BsuRI GGCC 4 cut(s) 80, 152, 486, 594
BtsCI GGATG 1 cut(s) 20
BtsIMutI CAGTG 1 cut(s) 274
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 2 cut(s) 129, 585
CfoI GCGC 1 cut(s) 111
Cfr13I GGNCC 3 cut(s) 30, 79, 158
Csp6I GTAC 2 cut(s) 35, 627
CviAII CATG 2 cut(s) 19, 547
CviQI GTAC 2 cut(s) 35, 627
DdeI CTNAG 3 cut(s) 83, 474, 642
DpnI GATC 1 cut(s) 318
DpnII GATC 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 183
Eco130I CCWWGG 1 cut(s) 513
Eco24I GRGCYC 1 cut(s) 248
Eco47I GGWCC 2 cut(s) 30, 158
Eco81I CCTNAGG 1 cut(s) 83
Eco88I CYCGRG 2 cut(s) 132, 599
EcoNI CCTNNNNNAGG 1 cut(s) 86
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 2 cut(s) 366, 613
EcoT14I CCWWGG 1 cut(s) 513
EcoT22I ATGCAT 1 cut(s) 20
EcoT38I GRGCYC 1 cut(s) 248
ErhI CCWWGG 1 cut(s) 513
FaeI CATG 2 cut(s) 22, 550
FaiI YATR 8 cut(s) 20, 55, 57, 326, 344, 437, 548, 611
FatI CATG 2 cut(s) 18, 546
FauNDI CATATG 1 cut(s) 55
Fnu4HI GCNGC 3 cut(s) 51, 220, 459
FokI GGATG 1 cut(s) 27
FriOI GRGCYC 1 cut(s) 248
Fsp4HI GCNGC 3 cut(s) 51, 220, 459
FspBI CTAG 2 cut(s) 300, 667
GlaI GCGC 1 cut(s) 110
GluI GCNGC 3 cut(s) 51, 220, 459
HaeIII GGCC 4 cut(s) 80, 152, 486, 594
HapII CCGG 1 cut(s) 149
HhaI GCGC 1 cut(s) 111
Hin1II CATG 2 cut(s) 22, 550
Hin6I GCGC 1 cut(s) 109
HinP1I GCGC 1 cut(s) 109
HinfI GANTC 1 cut(s) 263
HpaII CCGG 1 cut(s) 149
HphI GGTGA 1 cut(s) 534
Hpy188I TCNGA 1 cut(s) 321
Hpy188III TCNNGA 7 cut(s) 134, 156, 209, 300, 464, 535, 599
HpyAV CCTTC 3 cut(s) 461, 605, 643
HpyCH4III ACNGT 1 cut(s) 333
HpyCH4IV ACGT 1 cut(s) 355
HpyCH4V TGCA 4 cut(s) 18, 53, 203, 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 106, 564
HpyF3I CTNAG 3 cut(s) 83, 474, 642
HpySE526I ACGT 1 cut(s) 355
Hsp92II CATG 2 cut(s) 22, 550
HspAI GCGC 1 cut(s) 109
Kzo9I GATC 1 cut(s) 316
Lsp1109I GCAGC 3 cut(s) 37, 231, 445
LweI GCATC 2 cut(s) 5, 37
MaeI CTAG 2 cut(s) 300, 667
MaeII ACGT 1 cut(s) 355
MalI GATC 1 cut(s) 318
MboI GATC 1 cut(s) 316
MboII GAAGA 3 cut(s) 149, 200, 288
MhlI GDGCHC 2 cut(s) 181, 248
MluCI AATT 1 cut(s) 290
MlyI GAGTC 1 cut(s) 272
MnlI CCTC 9 cut(s) 70, 82, 163, 184, 339, 342, 399, 501, 646
Mph1103I ATGCAT 1 cut(s) 20
MslI CAYNNNNRTG 1 cut(s) 58
MspA1I CMGCKG 2 cut(s) 67, 583
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 2 cut(s) 368, 615
Mva1269I GAATGC 1 cut(s) 460
MvaI CCWGG 2 cut(s) 368, 615
MwoI GCNNNNNNNGC 3 cut(s) 50, 106, 564
NdeI CATATG 1 cut(s) 55
NdeII GATC 1 cut(s) 316
NlaIII CATG 2 cut(s) 22, 550
NlaIV GGNNCC 2 cut(s) 31, 499
NsiI ATGCAT 1 cut(s) 20
NspI RCATGY 1 cut(s) 22
PctI GAATGC 1 cut(s) 460
PkrI GCNGC 3 cut(s) 52, 221, 460
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
Psp6I CCWGG 2 cut(s) 366, 613
PspGI CCWGG 2 cut(s) 366, 613
PspN4I GGNNCC 2 cut(s) 31, 499
PspPI GGNCC 3 cut(s) 30, 79, 158
PvuII CAGCTG 1 cut(s) 583
RsaI GTAC 2 cut(s) 36, 628
RsaNI GTAC 2 cut(s) 35, 627
RseI CAYNNNNRTG 1 cut(s) 58
SatI GCNGC 3 cut(s) 51, 220, 459
Sau3AI GATC 1 cut(s) 316
Sau96I GGNCC 3 cut(s) 30, 79, 158
ScaI AGTACT 1 cut(s) 628
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 2 cut(s) 368, 615
SduI GDGCHC 2 cut(s) 181, 248
SetI ASST 9 cut(s) 40, 46, 208, 358, 369, 423, 565, 585, 657
SfaNI GCATC 2 cut(s) 5, 37
SfcI CTRYAG 2 cut(s) 39, 435
SinI GGWCC 2 cut(s) 30, 158
SmiMI CAYNNNNRTG 1 cut(s) 58
SmlI CTYRAG 1 cut(s) 462
SmoI CTYRAG 1 cut(s) 462
Sse9I AATT 1 cut(s) 290
SsiI CCGC 1 cut(s) 65
SspMI CTAG 2 cut(s) 300, 667
StyD4I CCNGG 2 cut(s) 366, 613
StyI CCWWGG 1 cut(s) 513
TaaI ACNGT 1 cut(s) 333
TaiI ACGT 1 cut(s) 358
TaqI TCGA 2 cut(s) 315, 391
TasI AATT 1 cut(s) 290
TatI WGTACW 1 cut(s) 626
TscAI CASTG 1 cut(s) 274
TseI GCWGC 3 cut(s) 50, 219, 458
TspDTI ATGAA 5 cut(s) 17, 72, 303, 467, 563
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 1 cut(s) 274
VpaK11BI GGWCC 2 cut(s) 30, 158
XagI CCTNNNNNAGG 1 cut(s) 86
XbaI TCTAGA 1 cut(s) 299
XceI RCATGY 1 cut(s) 22
XspI CTAG 2 cut(s) 300, 667
ZrmI AGTACT 1 cut(s) 628
Zsp2I ATGCAT 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.