Rorug01G0208500

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000336
Physical Location & Seq
Reverse (-)
29415363 .. 29416156
794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0208500.1

Sequence Viewer

Length: 561 bp
ATGACAACTCCCATATTCACCTTCACATTCCTCCCTCTCTTCCTCCTCTTCTCATCAACCTTCACTTCCATAACTGCCAACAATGTCATCTCTGAAACCTGCAAGGAATGTGCTCAAAAAAACCCCAGCCTGAGCTACAAGTTCTGCGTGACTTCTCTCCAAGCCGACCCAAACAGCCAAGGCGCCGATCTCCGGCAACTAGGCCTCATCTCCATGAAGCTAGTCCAGCACAACGTGACCAACACAAGGCGCTTCATCAAGCATCTTCTCAAGAACGAGGAGCTTGACAAGTTCGTAAGGATGTATTTGGATGACTGTTTGGACCTTTACACAGACGCCATACCTAGCATCAAGCAAGCCGTCAAGGATTACAAAATGAAGGACAACAGACACGCCAATATCGAAGTGAGTTCGGTCGCCGGTGCCGCCACGACTTGCGAAGATGGATTCGAAGAGAGACCAGGGTTGGTTTCGCCATTAACGAAGAGGAACAACGAGATGTTTCAGCTGTGTGTTATAGCGCTTAGGATTATCAATATGGTCAGTTCGCCGTTTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

186

Amino Acids

21.05

Weight (kDa)

7.59

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 29 - 177 4.6e-27 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000336)

Species Orthologous Gene IDs
malus_domestica MD05G1024100.v1.1
rosa_chinensis RchiOBHm_Chr2g0152291 RchiOBHm_Chr4g0387411
rosa_laevigata RLG00000009698 RLG00000009699 RLG00000009700 RLG00000023615 RLG00000024265 RLG00000034445 RLG00000036567
rosa_multiflora Rmu_co8268067.1_g000001 Rmu_co8313555.1_g000001 Rmu_co8324697.1_g000001 Rmu_sc0000318.1_g000023 Rmu_sc0000342.1_g000016 Rmu_sc0000447.1_g000010 Rmu_sc0000528.1_g000009 Rmu_sc0000539.1_g000060 Rmu_sc0000929.1_g000023 Rmu_sc0001493.1_g000081 Rmu_sc0001674.1_g000003 Rmu_sc0001791.1_g000023 Rmu_sc0001822.1_g000005 Rmu_sc0001822.1_g000033 Rmu_sc0002061.1_g000010 Rmu_sc0002286.1_g000012 Rmu_sc0002979.1_g000009 Rmu_sc0003158.1_g000016 Rmu_sc0003221.1_g000001 Rmu_sc0003641.1_g000029 Rmu_sc0003742.1_g000012 Rmu_sc0003814.1_g000004 Rmu_sc0003823.1_g000005 Rmu_sc0004002.1_g000005 Rmu_sc0004564.1_g000005 Rmu_sc0005415.1_g000003 Rmu_sc0006050.1_g000025 Rmu_sc0006555.1_g000002 Rmu_sc0009954.1_g000020 Rmu_sc0010421.1_g000007 Rmu_sc0010607.1_g000002 Rmu_sc0011044.1_g000005 Rmu_sc0011497.1_g000015 Rmu_sc0014532.1_g000004 Rmu_sc0014745.1_g000005 Rmu_sc0014821.1_g000001 Rmu_sc0015038.1_g000002 Rmu_sc0015454.1_g000004 Rmu_sc0019663.1_g000002 Rmu_sc0027475.1_g000003 Rmu_sc0028826.1_g000002 Rmu_ssc0000117.1_g000019 Rmu_ssc0000175.1_g000034
rosa_roxburghii Rroxscaffold_3G00237080 Rroxscaffold_4G00288290 Rroxscaffold_6G00397510
rosa_rugosa Rorug01G0035000 Rorug01G0190900 Rorug01G0208500 Rorug01G0208600 Rorug01G0208600 Rorug01G0344900 Rorug01G0479500 Rorug01G0479600 Rorug02G0230400 Rorug02G0320900 Rorug02G0433300 Rorug03G0147400 Rorug03G0169700.1 Rorug03G0169800 Rorug03G0198300 Rorug04G0022900 Rorug04G0064600 Rorug04G0322100 Rorug04G0388900 Rorug05G0153200 Rorug05G0231600 Rorug05G0231700 Rorug05G0231800 Rorug05G0276200 Rorug05G0292400 Rorug05G0314700 Rorug06G0092000 Rorug06G0260900 Rorug06G0273600 RorugPtG0003200
rosa_samantha Rh1AG264100 Rh1AG292500 Rh1AG423800 Rh2AG098000 Rh2AG232700 Rh2AG361100 Rh2AG372900 Rh2BG099200 Rh2BG246000 Rh2BG260600 Rh2BG378400 Rh3BG142300 Rh3BG252900 Rh3BG267700 Rh3DG302400 Rh5BG059800 Rh5BG320000 Rh5BG530500 Rh6AG089300 Rh6AG089400 Rh6AG127400 Rh6AG376700 Rh7CG488700 Rh7DG265900
rosa_wichuraiana Rw2G040640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 107
AccB1I GGYRCC 2 cut(s) 182, 422
AciI CCGC 1 cut(s) 426
AcyI GRCGYC 2 cut(s) 183, 336
AdeI CACNNNGTG 1 cut(s) 235
AfeI AGCGCT 1 cut(s) 522
AfiI CCNNNNNNNGG 2 cut(s) 192, 246
AjnI CCWGG 1 cut(s) 460
AluBI AGCT 4 cut(s) 135, 220, 283, 508
AluI AGCT 4 cut(s) 135, 220, 283, 508
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 1 cut(s) 451
Aor51HI AGCGCT 1 cut(s) 522
AoxI GGCC 1 cut(s) 202
AspLEI GCGC 3 cut(s) 185, 252, 523
AspS9I GGNCC 1 cut(s) 322
AsuHPI GGTGA 1 cut(s) 10
AsuII TTCGAA 1 cut(s) 450
AvaII GGWCC 1 cut(s) 322
BanI GGYRCC 2 cut(s) 182, 422
Bbv12I GWGCWC 1 cut(s) 115
BccI CCATC 1 cut(s) 437
BceAI ACGGC 2 cut(s) 344, 535
BciT130I CCWGG 1 cut(s) 462
BcoDI GTCTC 1 cut(s) 451
BfaI CTAG 3 cut(s) 200, 221, 345
BfoI RGCGCY 3 cut(s) 186, 253, 524
BfuAI ACCTGC 1 cut(s) 107
BisI GCNGC 1 cut(s) 426
BlsI GCNGC 1 cut(s) 427
Bme1390I CCNGG 1 cut(s) 462
Bme18I GGWCC 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 2 cut(s) 184, 424
BmrFI CCNGG 1 cut(s) 462
BmsI GCATC 2 cut(s) 271, 357
Bpu10I CCTNAGC 2 cut(s) 131, 524
Bpu14I TTCGAA 1 cut(s) 450
BpuEI CTTGAG 1 cut(s) 254
BsaHI GRCGYC 2 cut(s) 183, 336
BsaI GGTCTC 1 cut(s) 451
BsaJI CCNNGG 2 cut(s) 178, 461
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 246
Bse118I RCCGGY 1 cut(s) 419
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 2 cut(s) 178, 461
BseGI GGATG 2 cut(s) 306, 316
BseLI CCNNNNNNNGG 2 cut(s) 192, 246
BseMII CTCAG 1 cut(s) 122
BseRI GAGGAG 2 cut(s) 35, 293
BseYI CCCAGC 1 cut(s) 125
Bsh1285I CGRYCG 1 cut(s) 417
BshFI GGCC 1 cut(s) 204
BshNI GGYRCC 2 cut(s) 182, 422
BsiEI CGRYCG 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 115
BsiSI CCGG 2 cut(s) 193, 420
BslI CCNNNNNNNGG 2 cut(s) 192, 246
BsmAI GTCTC 1 cut(s) 451
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 451
Bsp119I TTCGAA 1 cut(s) 450
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 1 cut(s) 187
BspACI CCGC 1 cut(s) 426
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 1 cut(s) 123
BspLI GGNNCC 2 cut(s) 184, 424
BspMI ACCTGC 1 cut(s) 107
BspT104I TTCGAA 1 cut(s) 450
BspT107I GGYRCC 2 cut(s) 182, 422
BspTNI GGTCTC 1 cut(s) 451
BsrFI RCCGGY 1 cut(s) 419
BssAI RCCGGY 1 cut(s) 419
BssECI CCNNGG 2 cut(s) 178, 461
BssMI GATC 1 cut(s) 187
BssNI GRCGYC 2 cut(s) 183, 336
BssT1I CCWWGG 1 cut(s) 178
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 1 cut(s) 317
Bst6I CTCTTC 4 cut(s) 44, 53, 447, 479
BstACI GRCGYC 2 cut(s) 183, 336
BstBI TTCGAA 1 cut(s) 450
BstC8I GCNNGC 1 cut(s) 357
BstDEI CTNAG 2 cut(s) 131, 524
BstF5I GGATG 2 cut(s) 306, 316
BstH2I RGCGCY 3 cut(s) 186, 253, 524
BstHHI GCGC 3 cut(s) 185, 252, 523
BstKTI GATC 1 cut(s) 190
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 1 cut(s) 187
BstMCI CGRYCG 1 cut(s) 417
BstMWI GCNNNNNNNGC 2 cut(s) 226, 425
BstNI CCWGG 1 cut(s) 462
BstSCI CCNGG 1 cut(s) 460
BsuRI GGCC 1 cut(s) 204
BtsCI GGATG 2 cut(s) 306, 316
BveI ACCTGC 1 cut(s) 107
Cac8I GCNNGC 1 cut(s) 357
CfoI GCGC 3 cut(s) 185, 252, 523
Cfr10I RCCGGY 1 cut(s) 419
Cfr13I GGNCC 1 cut(s) 322
CseI GACGC 1 cut(s) 344
CviAII CATG 1 cut(s) 214
CviJI RGCY 9 cut(s) 129, 135, 164, 177, 204, 220, 283, 359, 508
CviKI_1 RGCY 9 cut(s) 129, 135, 164, 177, 204, 220, 283, 359, 508
DdeI CTNAG 2 cut(s) 131, 524
DinI GGCGCC 1 cut(s) 184
DpnI GATC 1 cut(s) 189
DpnII GATC 1 cut(s) 187
DraIII CACNNNGTG 1 cut(s) 235
Eam1104I CTCTTC 4 cut(s) 44, 53, 447, 479
EarI CTCTTC 4 cut(s) 44, 53, 447, 479
Eco130I CCWWGG 1 cut(s) 178
Eco147I AGGCCT 1 cut(s) 204
Eco31I GGTCTC 1 cut(s) 451
Eco47I GGWCC 1 cut(s) 322
Eco47III AGCGCT 1 cut(s) 522
EcoRII CCWGG 1 cut(s) 460
EcoT14I CCWWGG 1 cut(s) 178
EgeI GGCGCC 1 cut(s) 184
EheI GGCGCC 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 178
FaeI CATG 1 cut(s) 217
FaiI YATR 6 cut(s) 14, 71, 215, 341, 518, 539
FatI CATG 1 cut(s) 213
Fnu4HI GCNGC 1 cut(s) 426
FokI GGATG 2 cut(s) 313, 323
Fsp4HI GCNGC 1 cut(s) 426
FspBI CTAG 3 cut(s) 200, 221, 345
GlaI GCGC 3 cut(s) 184, 251, 522
GluI GCNGC 1 cut(s) 426
GsaI CCCAGC 1 cut(s) 129
HaeII RGCGCY 3 cut(s) 186, 253, 524
HaeIII GGCC 1 cut(s) 204
HapII CCGG 2 cut(s) 193, 420
HgaI GACGC 1 cut(s) 344
HhaI GCGC 3 cut(s) 185, 252, 523
Hin1I GRCGYC 2 cut(s) 183, 336
Hin1II CATG 1 cut(s) 217
Hin6I GCGC 3 cut(s) 183, 250, 521
HinP1I GCGC 3 cut(s) 183, 250, 521
HinfI GANTC 1 cut(s) 447
HpaII CCGG 2 cut(s) 193, 420
HphI GGTGA 1 cut(s) 10
Hpy188I TCNGA 1 cut(s) 94
Hpy188III TCNNGA 1 cut(s) 271
HpyAV CCTTC 3 cut(s) 31, 70, 373
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 1 cut(s) 102
HpyF10VI GCNNNNNNNGC 2 cut(s) 226, 425
HpyF3I CTNAG 2 cut(s) 131, 524
HpySE526I ACGT 1 cut(s) 234
Hsp92I GRCGYC 2 cut(s) 183, 336
Hsp92II CATG 1 cut(s) 217
HspAI GCGC 3 cut(s) 183, 250, 521
KasI GGCGCC 1 cut(s) 182
Kzo9I GATC 1 cut(s) 187
LmnI GCTCC 1 cut(s) 280
LpnPI CCDG 8 cut(s) 112, 139, 143, 206, 239, 433, 447, 474
LweI GCATC 2 cut(s) 271, 357
MaeI CTAG 3 cut(s) 200, 221, 345
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 2 cut(s) 148, 235
MalI GATC 1 cut(s) 189
MboI GATC 1 cut(s) 187
MboII GAAGA 6 cut(s) 31, 40, 257, 452, 464, 496
MhlI GDGCHC 1 cut(s) 115
Mly113I GGCGCC 1 cut(s) 183
MnlI CCTC 7 cut(s) 41, 45, 53, 56, 215, 271, 480
MseI TTAA 2 cut(s) 479, 559
MslI CAYNNNNRTG 1 cut(s) 212
MspA1I CMGCKG 1 cut(s) 508
MspI CCGG 2 cut(s) 193, 420
MspR9I CCNGG 1 cut(s) 462
MvaI CCWGG 1 cut(s) 462
MwoI GCNNNNNNNGC 2 cut(s) 226, 425
NarI GGCGCC 1 cut(s) 183
NdeII GATC 1 cut(s) 187
NlaIII CATG 1 cut(s) 217
NlaIV GGNNCC 2 cut(s) 184, 424
NmuCI GTSAC 2 cut(s) 148, 235
NspV TTCGAA 1 cut(s) 450
PceI AGGCCT 1 cut(s) 204
PcsI WCGNNNNNNNCGW 2 cut(s) 399, 479
PfeI GAWTC 1 cut(s) 447
PkrI GCNGC 1 cut(s) 427
PluTI GGCGCC 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 460
PspFI CCCAGC 1 cut(s) 125
PspGI CCWGG 1 cut(s) 460
PspN4I GGNNCC 2 cut(s) 184, 424
PspPI GGNCC 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 508
RseI CAYNNNNRTG 1 cut(s) 212
SaqAI TTAA 2 cut(s) 479, 559
SatI GCNGC 1 cut(s) 426
Sau3AI GATC 1 cut(s) 187
Sau96I GGNCC 1 cut(s) 322
ScrFI CCNGG 1 cut(s) 462
SduI GDGCHC 1 cut(s) 115
SfaNI GCATC 2 cut(s) 271, 357
SfoI GGCGCC 1 cut(s) 184
SfuI TTCGAA 1 cut(s) 450
SgrAI CRCCGGYG 1 cut(s) 419
SinI GGWCC 1 cut(s) 322
SmiMI CAYNNNNRTG 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
SseBI AGGCCT 1 cut(s) 204
SsiI CCGC 1 cut(s) 426
SspDI GGCGCC 1 cut(s) 182
SspMI CTAG 3 cut(s) 200, 221, 345
StuI AGGCCT 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 460
StyI CCWWGG 1 cut(s) 178
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 1 cut(s) 237
TaqI TCGA 2 cut(s) 402, 450
TaqII GACCGA 1 cut(s) 403
TauI GCSGC 1 cut(s) 428
TfiI GAWTC 1 cut(s) 447
Tru1I TTAA 2 cut(s) 479, 559
Tru9I TTAA 2 cut(s) 479, 559
TseFI GTSAC 2 cut(s) 148, 235
Tsp45I GTSAC 2 cut(s) 148, 235
TspDTI ATGAA 3 cut(s) 230, 244, 392
VpaK11BI GGWCC 1 cut(s) 322
XspI CTAG 3 cut(s) 200, 221, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.