Rorug05G0314700

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000336
Physical Location & Seq
Forward (+)
37327042 .. 37328005
964 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0314700.1

Sequence Viewer

Length: 699 bp
ATGGCCGACTCTAAATCTACAGTCAGCCTGAAGCTACTCATCGATACAAAGCGTCAAAAGGTTCTGCTTGCCGAAGCGGGTAAGGACTTTGTGGATTTTCTTTTCACCCTTCTCTCTTTGCCAGTTGGCACTGTTATTAGGCTTCTGTCAAAGGATGGCATGGTTGGTAGCTTTGGTAAACTGTATGACAGTGTTGAAAATCTAGATTTAACATATATGGAACCCAAACTAAACAAGGATATCCTGCTCAAACCCAAGGCACCTGTTGCTGGACCTAATATCCTCGGTTTGTTGGACTTGGCCAACGTTGACGAACCAGCTGCTAGAAATAAGCCTACCGCCCACCTTTATGTGACTGAAGATCCTAACGAACAATGTCCAAAGTGCAATCACCATATGTCTTCTCAGGCGACTTATGTTGGTCAACCAAAGACCACGGTAGCAGCTTCTCGCTTTGGTGCAGCAGGATATGTCAAAGGCCTAGTTACGTACATTATAATGGATGATCTGGAGGTGAAGCCTATGTCCACTATGTCAAGCATAACTCTGCTCAACAGGTTCAATGTTAAGGATGTTGGCACCCTCGAAGAGAAGGTTGTAGATCTAGGCATGAATGAGGGTCTGAAACTGCTGAAAGAATCCCTACAATCCAAAGCAGTTCTCACAAATGTGTTCCTGGGGAAGAAAGCTGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_3 zf-CCHC_4

Protein Analysis

232

Amino Acids

25.21

Weight (kDa)

8.37

Isoelectric Point (pI)

32.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 5 - 229 3.9e-45 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000336)

Species Orthologous Gene IDs
malus_domestica MD05G1024100.v1.1
rosa_chinensis RchiOBHm_Chr2g0152291 RchiOBHm_Chr4g0387411
rosa_laevigata RLG00000009698 RLG00000009699 RLG00000009700 RLG00000023615 RLG00000024265 RLG00000034445 RLG00000036567
rosa_multiflora Rmu_co8268067.1_g000001 Rmu_co8313555.1_g000001 Rmu_co8324697.1_g000001 Rmu_sc0000318.1_g000023 Rmu_sc0000342.1_g000016 Rmu_sc0000447.1_g000010 Rmu_sc0000528.1_g000009 Rmu_sc0000539.1_g000060 Rmu_sc0000929.1_g000023 Rmu_sc0001493.1_g000081 Rmu_sc0001674.1_g000003 Rmu_sc0001791.1_g000023 Rmu_sc0001822.1_g000005 Rmu_sc0001822.1_g000033 Rmu_sc0002061.1_g000010 Rmu_sc0002286.1_g000012 Rmu_sc0002979.1_g000009 Rmu_sc0003158.1_g000016 Rmu_sc0003221.1_g000001 Rmu_sc0003641.1_g000029 Rmu_sc0003742.1_g000012 Rmu_sc0003814.1_g000004 Rmu_sc0003823.1_g000005 Rmu_sc0004002.1_g000005 Rmu_sc0004564.1_g000005 Rmu_sc0005415.1_g000003 Rmu_sc0006050.1_g000025 Rmu_sc0006555.1_g000002 Rmu_sc0009954.1_g000020 Rmu_sc0010421.1_g000007 Rmu_sc0010607.1_g000002 Rmu_sc0011044.1_g000005 Rmu_sc0011497.1_g000015 Rmu_sc0014532.1_g000004 Rmu_sc0014745.1_g000005 Rmu_sc0014821.1_g000001 Rmu_sc0015038.1_g000002 Rmu_sc0015454.1_g000004 Rmu_sc0019663.1_g000002 Rmu_sc0027475.1_g000003 Rmu_sc0028826.1_g000002 Rmu_ssc0000117.1_g000019 Rmu_ssc0000175.1_g000034
rosa_roxburghii Rroxscaffold_3G00237080 Rroxscaffold_4G00288290 Rroxscaffold_6G00397510
rosa_rugosa Rorug01G0035000 Rorug01G0190900 Rorug01G0208500 Rorug01G0208600 Rorug01G0208600 Rorug01G0344900 Rorug01G0479500 Rorug01G0479600 Rorug02G0230400 Rorug02G0320900 Rorug02G0433300 Rorug03G0147400 Rorug03G0169700.1 Rorug03G0169800 Rorug03G0198300 Rorug04G0022900 Rorug04G0064600 Rorug04G0322100 Rorug04G0388900 Rorug05G0153200 Rorug05G0231600 Rorug05G0231700 Rorug05G0231800 Rorug05G0276200 Rorug05G0292400 Rorug05G0314700 Rorug06G0092000 Rorug06G0260900 Rorug06G0273600 RorugPtG0003200
rosa_samantha Rh1AG264100 Rh1AG292500 Rh1AG423800 Rh2AG098000 Rh2AG232700 Rh2AG361100 Rh2AG372900 Rh2BG099200 Rh2BG246000 Rh2BG260600 Rh2BG378400 Rh3BG142300 Rh3BG252900 Rh3BG267700 Rh3DG302400 Rh5BG059800 Rh5BG320000 Rh5BG530500 Rh6AG089300 Rh6AG089400 Rh6AG127400 Rh6AG376700 Rh7CG488700 Rh7DG265900
rosa_wichuraiana Rw2G040640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 497
AccB1I GGYRCC 2 cut(s) 259, 578
AciI CCGC 2 cut(s) 77, 339
AclI AACGTT 1 cut(s) 306
AclWI GGATC 1 cut(s) 356
AcoI YGGCCR 2 cut(s) 3, 300
AcuI CTGAAG 2 cut(s) 50, 378
AfaI GTAC 1 cut(s) 491
AfiI CCNNNNNNNGG 1 cut(s) 269
AgsI TTSAA 2 cut(s) 197, 562
AjnI CCWGG 1 cut(s) 675
AleI CACNNNNGTG 1 cut(s) 668
AluBI AGCT 5 cut(s) 34, 171, 320, 446, 689
AluI AGCT 5 cut(s) 34, 171, 320, 446, 689
AlwI GGATC 1 cut(s) 356
AoxI GGCC 3 cut(s) 3, 300, 478
ApeKI GCWGC 5 cut(s) 320, 443, 461, 689, 692
AspS9I GGNCC 1 cut(s) 272
AsuHPI GGTGA 3 cut(s) 97, 383, 526
AvaII GGWCC 1 cut(s) 272
BalI TGGCCA 1 cut(s) 302
BanI GGYRCC 2 cut(s) 259, 578
BbsI GAAGAC 1 cut(s) 393
BbvI GCAGC 5 cut(s) 307, 455, 473, 676, 679
BccI CCATC 1 cut(s) 149
BcgI CGANNNNNNTGC 2 cut(s) 302, 336
BciT130I CCWGG 1 cut(s) 677
BfaI CTAG 4 cut(s) 203, 324, 482, 605
BfmI CTRYAG 1 cut(s) 18
BglII AGATCT 1 cut(s) 601
BisI GCNGC 5 cut(s) 321, 444, 462, 690, 693
BlsI GCNGC 5 cut(s) 322, 445, 463, 691, 694
Bme1390I CCNGG 1 cut(s) 677
Bme18I GGWCC 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 3 cut(s) 222, 261, 580
BmrFI CCNGG 1 cut(s) 677
BpiI GAAGAC 1 cut(s) 393
BpmI CTGGAG 1 cut(s) 530
Bsa29I ATCGAT 1 cut(s) 42
BsaAI YACGTR 1 cut(s) 489
BsaJI CCNNGG 4 cut(s) 255, 283, 435, 676
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse1I ACTGG 1 cut(s) 122
BseBI CCWGG 1 cut(s) 677
BseCI ATCGAT 1 cut(s) 42
BseDI CCNNGG 4 cut(s) 255, 283, 435, 676
BseGI GGATG 3 cut(s) 160, 508, 577
BseLI CCNNNNNNNGG 1 cut(s) 269
BseMII CTCAG 1 cut(s) 419
BseNI ACTGG 1 cut(s) 122
BseXI GCAGC 5 cut(s) 307, 455, 473, 676, 679
BsgI GTGCAG 1 cut(s) 480
BshFI GGCC 3 cut(s) 5, 302, 480
BshNI GGYRCC 2 cut(s) 259, 578
BshVI ATCGAT 1 cut(s) 42
BslI CCNNNNNNNGG 1 cut(s) 269
BsnI GGCC 3 cut(s) 5, 302, 480
Bsp143I GATC 3 cut(s) 361, 505, 601
BspACI CCGC 2 cut(s) 77, 339
BspANI GGCC 3 cut(s) 5, 302, 480
BspCNI CTCAG 1 cut(s) 418
BspDI ATCGAT 1 cut(s) 42
BspLI GGNNCC 3 cut(s) 222, 261, 580
BspPI GGATC 1 cut(s) 356
BspT107I GGYRCC 2 cut(s) 259, 578
BsrI ACTGG 1 cut(s) 122
BssECI CCNNGG 4 cut(s) 255, 283, 435, 676
BssMI GATC 3 cut(s) 361, 505, 601
BssT1I CCWWGG 1 cut(s) 255
Bst2UI CCWGG 1 cut(s) 677
Bst4CI ACNGT 5 cut(s) 22, 133, 183, 191, 439
Bst6I CTCTTC 1 cut(s) 582
BstAPI GCANNNNNTGC 1 cut(s) 266
BstBAI YACGTR 1 cut(s) 489
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 1 cut(s) 405
BstDSI CCRYGG 1 cut(s) 435
BstF5I GGATG 3 cut(s) 160, 508, 577
BstKTI GATC 3 cut(s) 364, 508, 604
BstMBI GATC 3 cut(s) 361, 505, 601
BstMWI GCNNNNNNNGC 1 cut(s) 266
BstNI CCWGG 1 cut(s) 677
BstSCI CCNGG 1 cut(s) 675
BstSFI CTRYAG 1 cut(s) 18
BstSNI TACGTA 1 cut(s) 489
BstV1I GCAGC 5 cut(s) 307, 455, 473, 676, 679
BstV2I GAAGAC 1 cut(s) 393
BstX2I RGATCY 2 cut(s) 361, 601
BstYI RGATCY 2 cut(s) 361, 601
Bsu15I ATCGAT 1 cut(s) 42
BsuRI GGCC 3 cut(s) 5, 302, 480
BsuTUI ATCGAT 1 cut(s) 42
BtgI CCRYGG 1 cut(s) 435
BtsCI GGATG 3 cut(s) 160, 508, 577
BtsIMutI CAGTG 2 cut(s) 129, 196
Cac8I GCNNGC 1 cut(s) 69
Cfr13I GGNCC 1 cut(s) 272
ClaI ATCGAT 1 cut(s) 42
CseI GACGC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 490
CviAII CATG 3 cut(s) 160, 610, 696
CviQI GTAC 1 cut(s) 490
DdeI CTNAG 1 cut(s) 405
DpnI GATC 3 cut(s) 363, 507, 603
DpnII GATC 3 cut(s) 361, 505, 601
EaeI YGGCCR 2 cut(s) 3, 300
Eam1104I CTCTTC 1 cut(s) 582
EarI CTCTTC 1 cut(s) 582
Eco105I TACGTA 1 cut(s) 489
Eco130I CCWWGG 1 cut(s) 255
Eco147I AGGCCT 1 cut(s) 480
Eco32I GATATC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 272
Eco57I CTGAAG 2 cut(s) 50, 378
EcoRII CCWGG 1 cut(s) 675
EcoRV GATATC 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 3 cut(s) 163, 613, 699
FatI CATG 3 cut(s) 159, 609, 695
FauI CCCGC 1 cut(s) 70
FauNDI CATATG 1 cut(s) 396
Fnu4HI GCNGC 5 cut(s) 321, 444, 462, 690, 693
FokI GGATG 3 cut(s) 167, 515, 584
Fsp4HI GCNGC 5 cut(s) 321, 444, 462, 690, 693
FspBI CTAG 4 cut(s) 203, 324, 482, 605
GluI GCNGC 5 cut(s) 321, 444, 462, 690, 693
GsuI CTGGAG 1 cut(s) 530
HaeIII GGCC 3 cut(s) 5, 302, 480
HgaI GACGC 1 cut(s) 41
Hin1II CATG 3 cut(s) 163, 613, 699
HincII GTYRAC 2 cut(s) 310, 425
HindII GTYRAC 2 cut(s) 310, 425
HinfI GANTC 2 cut(s) 8, 638
HphI GGTGA 3 cut(s) 97, 383, 526
Hpy166II GTNNAC 4 cut(s) 179, 310, 425, 528
Hpy188I TCNGA 1 cut(s) 624
Hpy188III TCNNGA 2 cut(s) 203, 509
Hpy8I GTNNAC 4 cut(s) 179, 310, 425, 528
HpyAV CCTTC 2 cut(s) 119, 586
HpyCH4III ACNGT 5 cut(s) 22, 133, 183, 191, 439
HpyCH4IV ACGT 2 cut(s) 306, 488
HpyCH4V TGCA 3 cut(s) 387, 461, 695
HpyF10VI GCNNNNNNNGC 1 cut(s) 266
HpyF3I CTNAG 1 cut(s) 405
HpySE526I ACGT 2 cut(s) 306, 488
Hsp92II CATG 3 cut(s) 163, 613, 699
Kzo9I GATC 3 cut(s) 361, 505, 601
Lsp1109I GCAGC 5 cut(s) 307, 455, 473, 676, 679
MaeI CTAG 4 cut(s) 203, 324, 482, 605
MaeII ACGT 2 cut(s) 306, 488
MaeIII GTNAC 2 cut(s) 352, 484
MalI GATC 3 cut(s) 363, 507, 603
MboI GATC 3 cut(s) 361, 505, 601
MboII GAAGA 4 cut(s) 371, 393, 599, 694
MflI RGATCY 2 cut(s) 361, 601
MlsI TGGCCA 1 cut(s) 302
MluNI TGGCCA 1 cut(s) 302
MlyI GAGTC 1 cut(s) 2
MmeI TCCRAC 1 cut(s) 273
MnlI CCTC 4 cut(s) 293, 505, 593, 610
Mox20I TGGCCA 1 cut(s) 302
MscI TGGCCA 1 cut(s) 302
MseI TTAA 2 cut(s) 209, 567
MslI CAYNNNNRTG 3 cut(s) 348, 497, 668
Msp20I TGGCCA 1 cut(s) 302
MspA1I CMGCKG 1 cut(s) 320
MspR9I CCNGG 1 cut(s) 677
MvaI CCWGG 1 cut(s) 677
MwoI GCNNNNNNNGC 1 cut(s) 266
NdeI CATATG 1 cut(s) 396
NdeII GATC 3 cut(s) 361, 505, 601
NlaIII CATG 3 cut(s) 163, 613, 699
NlaIV GGNNCC 3 cut(s) 222, 261, 580
NmuCI GTSAC 1 cut(s) 352
OliI CACNNNNGTG 1 cut(s) 668
PceI AGGCCT 1 cut(s) 480
PfeI GAWTC 1 cut(s) 638
PkrI GCNGC 5 cut(s) 322, 445, 463, 691, 694
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
Ppu21I YACGTR 1 cut(s) 489
PsiI TTATAA 1 cut(s) 497
Psp1406I AACGTT 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 675
PspGI CCWGG 1 cut(s) 675
PspN4I GGNNCC 3 cut(s) 222, 261, 580
PspPI GGNCC 1 cut(s) 272
PsuI RGATCY 2 cut(s) 361, 601
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
RseI CAYNNNNRTG 3 cut(s) 348, 497, 668
SaqAI TTAA 2 cut(s) 209, 567
SatI GCNGC 5 cut(s) 321, 444, 462, 690, 693
Sau3AI GATC 3 cut(s) 361, 505, 601
Sau96I GGNCC 1 cut(s) 272
SchI GAGTC 1 cut(s) 2
ScrFI CCNGG 1 cut(s) 677
SfcI CTRYAG 1 cut(s) 18
SinI GGWCC 1 cut(s) 272
SmiMI CAYNNNNRTG 3 cut(s) 348, 497, 668
SnaBI TACGTA 1 cut(s) 489
SseBI AGGCCT 1 cut(s) 480
SsiI CCGC 2 cut(s) 77, 339
SspMI CTAG 4 cut(s) 203, 324, 482, 605
StuI AGGCCT 1 cut(s) 480
StyD4I CCNGG 1 cut(s) 675
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 5 cut(s) 22, 133, 183, 191, 439
TaiI ACGT 2 cut(s) 309, 491
TaqI TCGA 2 cut(s) 42, 585
TfiI GAWTC 1 cut(s) 638
Tru1I TTAA 2 cut(s) 209, 567
Tru9I TTAA 2 cut(s) 209, 567
TscAI CASTG 2 cut(s) 136, 196
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 5 cut(s) 320, 443, 461, 689, 692
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 1 cut(s) 626
TspRI CASTG 2 cut(s) 136, 196
VpaK11BI GGWCC 1 cut(s) 272
XbaI TCTAGA 1 cut(s) 202
XspI CTAG 4 cut(s) 203, 324, 482, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.