Rorug02G0230400

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000336
Physical Location & Seq
Forward (+)
22969527 .. 22971351
1825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0230400.1

Sequence Viewer

Length: 1203 bp
ATGAACCGCATTATTAAACTGGCTCACCGTGATGGAAGGACAGTCATTTCATCAATTCATCAACCTAGCAGTGAAGTGTTTGAGCTTTTCCAAAATCTTTGTCTTCTCTCCTCTGGTAGAACAGTCTATTTCGGCCCTGCTTCAGCGGCAGAACAGTTTTTTGCTTTAAATGGCTTCCCACGCCCAGCTCTGAGAAACCCATCAGATCACTGCCTGAGAACTATAAACAAGGATTTTGATAGTGACATTGAACAAGGGCTTGATGGAAAAACAAGCACCGAGGAAGCAATTGAGATTCTCATAAATTCATACAAGTCTTCAGGAACTTTCAAGCAACTTGAGCACCGAGTAACTGAAATTTGCCAACAGAAAGGTGGAGCTCTAATGAAAGGAAGCCAAGCCAACTTTATAACTCAATTAATGCCTGGTTTTGACAAGGAGATCATTCGGACTATTTATCATGAAATTGACTCCACTTTTGACTCAATACAGGATAGAGCATCAATGCTCATGTTTGTTGCAGCATTCTTAACTTTCATGGCAATAGGTGGATTTCCTTCTTTTGTAGAAGACATGAAGATTTTCGAGCGTGAAAGATTAAACGGGCACTATGGTGTTGCTGCATTTGCTGTTGGAAACTCATTTTCTTCCATTCCATACTTACTGATGATCTCTTTAATTCCTAGAGCAATAGCCTATTTCCTTGTTGGCCTTCAGAAGAGCTTTGACCACTTCACCTATTTTGCATTGGTTCTGTTTGTGTGTCTGATGTTGGTTGAGAGCCTGATGATGATTGTAGCAAGCATTGTGCTAGATTTCCTCATGGGTGTTATCACAGGTTCTGGAATTCTAGGAGTGATGTTGTTGAATGGAGGTTTCTTCCGAAAACCTCACGATCTTCCAAACCCCTTCTGGAGATACCCAATGTACTATATTGGATTCCACAAGTATGTAAATCAAGGATTCTACAAGAATGAGTTTGAAGGACTAACATTTCCCAGTAATCAAGCAGGAGGGCCACCCATCATTACTGGTGAAGAAATATTGAAGAGTATTTGGCAAATGGAGATGGGCTACTCTAAGTGGGTTGATCTTGCCATTTTGTTTGGAATGGTAATTGTTTACAGGCTCATGTTCTTTGGAATCATCAAGACAAGTGAAAAGGTCGCACCAATCATCAAAGCTCTTCTTAACAGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

400

Amino Acids

45.04

Weight (kDa)

6.51

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC2_membrane_7 PF19055 20 - 165 1.3e-07 ABC-2 type transporter
ABC2_membrane PF01061 148 - 327 1.4e-32 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000336)

Species Orthologous Gene IDs
malus_domestica MD05G1024100.v1.1
rosa_chinensis RchiOBHm_Chr2g0152291 RchiOBHm_Chr4g0387411
rosa_laevigata RLG00000009698 RLG00000009699 RLG00000009700 RLG00000023615 RLG00000024265 RLG00000034445 RLG00000036567
rosa_multiflora Rmu_co8268067.1_g000001 Rmu_co8313555.1_g000001 Rmu_co8324697.1_g000001 Rmu_sc0000318.1_g000023 Rmu_sc0000342.1_g000016 Rmu_sc0000447.1_g000010 Rmu_sc0000528.1_g000009 Rmu_sc0000539.1_g000060 Rmu_sc0000929.1_g000023 Rmu_sc0001493.1_g000081 Rmu_sc0001674.1_g000003 Rmu_sc0001791.1_g000023 Rmu_sc0001822.1_g000005 Rmu_sc0001822.1_g000033 Rmu_sc0002061.1_g000010 Rmu_sc0002286.1_g000012 Rmu_sc0002979.1_g000009 Rmu_sc0003158.1_g000016 Rmu_sc0003221.1_g000001 Rmu_sc0003641.1_g000029 Rmu_sc0003742.1_g000012 Rmu_sc0003814.1_g000004 Rmu_sc0003823.1_g000005 Rmu_sc0004002.1_g000005 Rmu_sc0004564.1_g000005 Rmu_sc0005415.1_g000003 Rmu_sc0006050.1_g000025 Rmu_sc0006555.1_g000002 Rmu_sc0009954.1_g000020 Rmu_sc0010421.1_g000007 Rmu_sc0010607.1_g000002 Rmu_sc0011044.1_g000005 Rmu_sc0011497.1_g000015 Rmu_sc0014532.1_g000004 Rmu_sc0014745.1_g000005 Rmu_sc0014821.1_g000001 Rmu_sc0015038.1_g000002 Rmu_sc0015454.1_g000004 Rmu_sc0019663.1_g000002 Rmu_sc0027475.1_g000003 Rmu_sc0028826.1_g000002 Rmu_ssc0000117.1_g000019 Rmu_ssc0000175.1_g000034
rosa_roxburghii Rroxscaffold_3G00237080 Rroxscaffold_4G00288290 Rroxscaffold_6G00397510
rosa_rugosa Rorug01G0035000 Rorug01G0190900 Rorug01G0208500 Rorug01G0208600 Rorug01G0208600 Rorug01G0344900 Rorug01G0479500 Rorug01G0479600 Rorug02G0230400 Rorug02G0320900 Rorug02G0433300 Rorug03G0147400 Rorug03G0169700.1 Rorug03G0169800 Rorug03G0198300 Rorug04G0022900 Rorug04G0064600 Rorug04G0322100 Rorug04G0388900 Rorug05G0153200 Rorug05G0231600 Rorug05G0231700 Rorug05G0231800 Rorug05G0276200 Rorug05G0292400 Rorug05G0314700 Rorug06G0092000 Rorug06G0260900 Rorug06G0273600 RorugPtG0003200
rosa_samantha Rh1AG264100 Rh1AG292500 Rh1AG423800 Rh2AG098000 Rh2AG232700 Rh2AG361100 Rh2AG372900 Rh2BG099200 Rh2BG246000 Rh2BG260600 Rh2BG378400 Rh3BG142300 Rh3BG252900 Rh3BG267700 Rh3DG302400 Rh5BG059800 Rh5BG320000 Rh5BG530500 Rh6AG089300 Rh6AG089400 Rh6AG127400 Rh6AG376700 Rh7CG488700 Rh7DG265900
rosa_wichuraiana Rw2G040640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 410
AciI CCGC 2 cut(s) 7, 146
AcsI RAATTY 3 cut(s) 304, 357, 846
AcuI CTGAAG 3 cut(s) 126, 303, 698
AfaI GTAC 1 cut(s) 929
AgsI TTSAA 5 cut(s) 251, 331, 868, 983, 1048
AjnI CCWGG 1 cut(s) 424
AleI CACNNNNGTG 1 cut(s) 612
AluBI AGCT 5 cut(s) 85, 188, 380, 723, 1184
AluI AGCT 5 cut(s) 85, 188, 380, 723, 1184
Alw21I GWGCWC 2 cut(s) 345, 382
AlwNI CAGNNNCTG 1 cut(s) 842
AoxI GGCC 3 cut(s) 133, 709, 1016
ApeKI GCWGC 2 cut(s) 521, 620
ApoI RAATTY 3 cut(s) 304, 357, 846
ArsI GACNNNNNNTTYG 4 cut(s) 85, 117, 461, 493
AseI ATTAAT 1 cut(s) 419
Asp700I GAANNNNTTC 1 cut(s) 581
AspS9I GGNCC 2 cut(s) 134, 1016
AsuHPI GGTGA 3 cut(s) 17, 727, 1046
BaeGI GKGCMC 1 cut(s) 609
BanII GRGCYC 1 cut(s) 382
BbsI GAAGAC 3 cut(s) 95, 309, 576
Bbv12I GWGCWC 2 cut(s) 345, 382
BbvI GCAGC 2 cut(s) 533, 607
BccI CCATC 5 cut(s) 26, 208, 257, 1031, 1063
BciT130I CCWGG 1 cut(s) 426
BfaI CTAG 4 cut(s) 66, 684, 812, 851
BisI GCNGC 3 cut(s) 147, 522, 621
BlsI GCNGC 3 cut(s) 148, 523, 622
Bme1390I CCNGG 1 cut(s) 426
BmgT120I GGNCC 2 cut(s) 134, 1016
BmrFI CCNGG 1 cut(s) 426
BmrI ACTGGG 1 cut(s) 993
BmsI GCATC 1 cut(s) 509
BmuI ACTGGG 1 cut(s) 993
BpiI GAAGAC 3 cut(s) 95, 309, 576
BpmI CTGGAG 1 cut(s) 934
BpuEI CTTGAG 1 cut(s) 359
BsaJI CCNNGG 1 cut(s) 279
Bse1I ACTGG 3 cut(s) 24, 999, 1036
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 1 cut(s) 279
BseMII CTCAG 2 cut(s) 182, 206
BseNI ACTGG 3 cut(s) 24, 999, 1036
BseRI GAGGAG 1 cut(s) 100
BseSI GKGCMC 1 cut(s) 609
BseXI GCAGC 2 cut(s) 533, 607
BseYI CCCAGC 1 cut(s) 184
BshFI GGCC 3 cut(s) 135, 711, 1018
BsiHKAI GWGCWC 2 cut(s) 345, 382
BsmI GAATGC 1 cut(s) 524
BsnI GGCC 3 cut(s) 135, 711, 1018
Bsp1286I GDGCHC 3 cut(s) 345, 382, 609
Bsp143I GATC 5 cut(s) 205, 441, 669, 895, 1090
BspACI CCGC 2 cut(s) 7, 146
BspANI GGCC 3 cut(s) 135, 711, 1018
BspCNI CTCAG 2 cut(s) 183, 207
BspHI TCATGA 1 cut(s) 460
BspQI GCTCTTC 2 cut(s) 713, 1191
BsrI ACTGG 3 cut(s) 24, 999, 1036
BssECI CCNNGG 1 cut(s) 279
BssMI GATC 5 cut(s) 205, 441, 669, 895, 1090
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 5 cut(s) 29, 43, 124, 156, 1196
Bst6I CTCTTC 3 cut(s) 713, 1043, 1191
BstC8I GCNNGC 1 cut(s) 802
BstDEI CTNAG 3 cut(s) 191, 215, 1080
BstKTI GATC 5 cut(s) 208, 444, 672, 898, 1093
BstMBI GATC 5 cut(s) 205, 441, 669, 895, 1090
BstMWI GCNNNNNNNGC 4 cut(s) 146, 180, 340, 626
BstNI CCWGG 1 cut(s) 426
BstSCI CCNGG 1 cut(s) 424
BstSLI GKGCMC 1 cut(s) 609
BstV1I GCAGC 2 cut(s) 533, 607
BstV2I GAAGAC 3 cut(s) 95, 309, 576
BsuRI GGCC 3 cut(s) 135, 711, 1018
BtsI GCAGTG 2 cut(s) 76, 208
BtsIMutI CAGTG 2 cut(s) 76, 208
Cac8I GCNNGC 1 cut(s) 802
CaiI CAGNNNCTG 1 cut(s) 842
CciI TCATGA 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 134, 1016
Csp6I GTAC 1 cut(s) 928
CviAII CATG 6 cut(s) 461, 511, 538, 574, 823, 1132
CviQI GTAC 1 cut(s) 928
DdeI CTNAG 3 cut(s) 191, 215, 1080
DpnI GATC 5 cut(s) 207, 443, 671, 897, 1092
DpnII GATC 5 cut(s) 205, 441, 669, 895, 1090
DraI TTTAAA 1 cut(s) 168
Eam1104I CTCTTC 3 cut(s) 713, 1043, 1191
EarI CTCTTC 3 cut(s) 713, 1043, 1191
Ecl136II GAGCTC 1 cut(s) 380
Eco24I GRGCYC 1 cut(s) 382
Eco53kI GAGCTC 1 cut(s) 380
Eco57I CTGAAG 3 cut(s) 126, 303, 698
EcoICRI GAGCTC 1 cut(s) 380
EcoRI GAATTC 1 cut(s) 846
EcoRII CCWGG 1 cut(s) 424
EcoT38I GRGCYC 1 cut(s) 382
FaeI CATG 6 cut(s) 464, 514, 541, 577, 826, 1135
FalI AAGNNNNNCTT 1 cut(s) 1173
FatI CATG 6 cut(s) 460, 510, 537, 573, 822, 1131
Fnu4HI GCNGC 3 cut(s) 147, 522, 621
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 3 cut(s) 147, 522, 621
FspBI CTAG 4 cut(s) 66, 684, 812, 851
GluI GCNGC 3 cut(s) 147, 522, 621
GsaI CCCAGC 1 cut(s) 188
GsuI CTGGAG 1 cut(s) 934
HaeIII GGCC 3 cut(s) 135, 711, 1018
Hin1II CATG 6 cut(s) 464, 514, 541, 577, 826, 1135
HinfI GANTC 6 cut(s) 295, 470, 482, 939, 963, 1143
HphI GGTGA 3 cut(s) 17, 727, 1046
Hpy166II GTNNAC 1 cut(s) 1123
Hpy188I TCNGA 6 cut(s) 192, 205, 450, 717, 768, 884
Hpy188III TCNNGA 6 cut(s) 321, 461, 843, 893, 913, 1150
Hpy8I GTNNAC 1 cut(s) 1123
HpyAV CCTTC 5 cut(s) 30, 567, 722, 919, 977
HpyCH4III ACNGT 5 cut(s) 29, 43, 124, 156, 1196
HpyCH4V TGCA 3 cut(s) 521, 623, 746
HpyF10VI GCNNNNNNNGC 4 cut(s) 146, 180, 340, 626
HpyF3I CTNAG 3 cut(s) 191, 215, 1080
Hsp92II CATG 6 cut(s) 464, 514, 541, 577, 826, 1135
Kzo9I GATC 5 cut(s) 205, 441, 669, 895, 1090
LguI GCTCTTC 2 cut(s) 713, 1191
LmnI GCTCC 1 cut(s) 377
Lsp1109I GCAGC 2 cut(s) 533, 607
LweI GCATC 1 cut(s) 509
MaeI CTAG 4 cut(s) 66, 684, 812, 851
MaeIII GTNAC 2 cut(s) 242, 349
MalI GATC 5 cut(s) 207, 443, 671, 897, 1092
MboI GATC 5 cut(s) 205, 441, 669, 895, 1090
MfeI CAATTG 1 cut(s) 288
MhlI GDGCHC 3 cut(s) 345, 382, 609
MluCI AATT 9 cut(s) 54, 288, 304, 357, 416, 465, 678, 846, 1116
MlyI GAGTC 2 cut(s) 464, 476
MmeI TCCRAC 1 cut(s) 613
MnlI CCTC 6 cut(s) 121, 274, 830, 866, 900, 1007
MroXI GAANNNNTTC 1 cut(s) 581
MseI TTAA 8 cut(s) 15, 167, 419, 530, 599, 677, 1191, 1201
MslI CAYNNNNRTG 3 cut(s) 30, 612, 948
MspA1I CMGCKG 1 cut(s) 146
MspR9I CCNGG 1 cut(s) 426
MunI CAATTG 1 cut(s) 288
Mva1269I GAATGC 1 cut(s) 524
MvaI CCWGG 1 cut(s) 426
MwoI GCNNNNNNNGC 4 cut(s) 146, 180, 340, 626
NdeII GATC 5 cut(s) 205, 441, 669, 895, 1090
NlaIII CATG 6 cut(s) 464, 514, 541, 577, 826, 1135
NmuCI GTSAC 1 cut(s) 242
OliI CACNNNNGTG 1 cut(s) 612
PagI TCATGA 1 cut(s) 460
PciSI GCTCTTC 2 cut(s) 713, 1191
PctI GAATGC 1 cut(s) 524
PdmI GAANNNNTTC 1 cut(s) 581
PfeI GAWTC 4 cut(s) 295, 939, 963, 1143
PkrI GCNGC 3 cut(s) 148, 523, 622
PleI GAGTC 2 cut(s) 464, 476
PpsI GAGTC 2 cut(s) 464, 476
PshBI ATTAAT 1 cut(s) 419
PsiI TTATAA 1 cut(s) 410
Psp124BI GAGCTC 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 424
PspFI CCCAGC 1 cut(s) 184
PspGI CCWGG 1 cut(s) 424
PspPI GGNCC 2 cut(s) 134, 1016
PstNI CAGNNNCTG 1 cut(s) 842
RsaI GTAC 1 cut(s) 929
RsaNI GTAC 1 cut(s) 928
RseI CAYNNNNRTG 3 cut(s) 30, 612, 948
SacI GAGCTC 1 cut(s) 382
SapI GCTCTTC 2 cut(s) 713, 1191
SaqAI TTAA 8 cut(s) 15, 167, 419, 530, 599, 677, 1191, 1201
SatI GCNGC 3 cut(s) 147, 522, 621
Sau3AI GATC 5 cut(s) 205, 441, 669, 895, 1090
Sau96I GGNCC 2 cut(s) 134, 1016
SchI GAGTC 2 cut(s) 464, 476
ScrFI CCNGG 1 cut(s) 426
SduI GDGCHC 3 cut(s) 345, 382, 609
SfaNI GCATC 1 cut(s) 509
SmiMI CAYNNNNRTG 3 cut(s) 30, 612, 948
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
Sse9I AATT 9 cut(s) 54, 288, 304, 357, 416, 465, 678, 846, 1116
SsiI CCGC 2 cut(s) 7, 146
SspI AATATT 1 cut(s) 1044
SspMI CTAG 4 cut(s) 66, 684, 812, 851
SstI GAGCTC 1 cut(s) 382
StyD4I CCNGG 1 cut(s) 424
TaaI ACNGT 5 cut(s) 29, 43, 124, 156, 1196
TaqI TCGA 1 cut(s) 585
TasI AATT 9 cut(s) 54, 288, 304, 357, 416, 465, 678, 846, 1116
TatI WGTACW 1 cut(s) 927
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 4 cut(s) 295, 939, 963, 1143
Tru1I TTAA 8 cut(s) 15, 167, 419, 530, 599, 677, 1191, 1201
Tru9I TTAA 8 cut(s) 15, 167, 419, 530, 599, 677, 1191, 1201
TscAI CASTG 2 cut(s) 76, 215
TseFI GTSAC 1 cut(s) 242
TseI GCWGC 2 cut(s) 521, 620
Tsp45I GTSAC 1 cut(s) 242
TspDTI ATGAA 8 cut(s) 17, 39, 47, 297, 401, 477, 526, 590
TspRI CASTG 2 cut(s) 76, 215
VspI ATTAAT 1 cut(s) 419
XapI RAATTY 3 cut(s) 304, 357, 846
XcmI CCANNNNNNNNNTGG 2 cut(s) 371, 909
XmnI GAANNNNTTC 1 cut(s) 581
XspI CTAG 4 cut(s) 66, 684, 812, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.