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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfaI | GTAC | 1 cut(s) | 22 |
| AgsI | TTSAA | 1 cut(s) | 329 |
| AluBI | AGCT | 1 cut(s) | 357 |
| AluI | AGCT | 1 cut(s) | 357 |
| Alw26I | GTCTC | 1 cut(s) | 271 |
| AoxI | GGCC | 1 cut(s) | 310 |
| AspA2I | CCTAGG | 1 cut(s) | 179 |
| AsuHPI | GGTGA | 2 cut(s) | 299, 328 |
| AvrII | CCTAGG | 1 cut(s) | 179 |
| BaeGI | GKGCMC | 1 cut(s) | 138 |
| BccI | CCATC | 1 cut(s) | 152 |
| BcoDI | GTCTC | 1 cut(s) | 271 |
| BfaI | CTAG | 3 cut(s) | 36, 48, 180 |
| BlnI | CCTAGG | 1 cut(s) | 179 |
| BmcAI | AGTACT | 1 cut(s) | 22 |
| BmiI | GGNNCC | 1 cut(s) | 109 |
| BmsI | GCATC | 1 cut(s) | 205 |
| BsaI | GGTCTC | 1 cut(s) | 271 |
| BsaJI | CCNNGG | 2 cut(s) | 111, 179 |
| BseDI | CCNNGG | 2 cut(s) | 111, 179 |
| BseSI | GKGCMC | 1 cut(s) | 138 |
| BshFI | GGCC | 1 cut(s) | 312 |
| BsmAI | GTCTC | 1 cut(s) | 271 |
| BsnI | GGCC | 1 cut(s) | 312 |
| Bso31I | GGTCTC | 1 cut(s) | 271 |
| Bsp1286I | GDGCHC | 1 cut(s) | 138 |
| Bsp143I | GATC | 2 cut(s) | 289, 333 |
| BspANI | GGCC | 1 cut(s) | 312 |
| BspLI | GGNNCC | 1 cut(s) | 109 |
| BspQI | GCTCTTC | 1 cut(s) | 87 |
| BspTNI | GGTCTC | 1 cut(s) | 271 |
| BssECI | CCNNGG | 2 cut(s) | 111, 179 |
| BssMI | GATC | 2 cut(s) | 289, 333 |
| BssT1I | CCWWGG | 2 cut(s) | 111, 179 |
| Bst6I | CTCTTC | 1 cut(s) | 87 |
| BstDEI | CTNAG | 2 cut(s) | 171, 185 |
| BstKTI | GATC | 2 cut(s) | 292, 336 |
| BstMAI | GTCTC | 1 cut(s) | 271 |
| BstMBI | GATC | 2 cut(s) | 289, 333 |
| BstNSI | RCATGY | 1 cut(s) | 83 |
| BstSLI | GKGCMC | 1 cut(s) | 138 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 346 |
| BsuRI | GGCC | 1 cut(s) | 312 |
| BtsI | GCAGTG | 1 cut(s) | 109 |
| BtsIMutI | CAGTG | 1 cut(s) | 109 |
| Csp6I | GTAC | 1 cut(s) | 21 |
| CviAII | CATG | 1 cut(s) | 80 |
| CviJI | RGCY | 7 cut(s) | 47, 116, 184, 236, 247, 312, 357 |
| CviKI_1 | RGCY | 7 cut(s) | 47, 116, 184, 236, 247, 312, 357 |
| CviQI | GTAC | 1 cut(s) | 21 |
| DdeI | CTNAG | 2 cut(s) | 171, 185 |
| DpnI | GATC | 2 cut(s) | 291, 335 |
| DpnII | GATC | 2 cut(s) | 289, 333 |
| Eam1104I | CTCTTC | 1 cut(s) | 87 |
| EarI | CTCTTC | 1 cut(s) | 87 |
| Eco130I | CCWWGG | 2 cut(s) | 111, 179 |
| Eco31I | GGTCTC | 1 cut(s) | 271 |
| EcoT14I | CCWWGG | 2 cut(s) | 111, 179 |
| ErhI | CCWWGG | 2 cut(s) | 111, 179 |
| FaeI | CATG | 1 cut(s) | 83 |
| FaiI | YATR | 2 cut(s) | 81, 227 |
| FatI | CATG | 1 cut(s) | 79 |
| FspBI | CTAG | 3 cut(s) | 36, 48, 180 |
| HaeIII | GGCC | 1 cut(s) | 312 |
| Hin1II | CATG | 1 cut(s) | 83 |
| HphI | GGTGA | 2 cut(s) | 299, 328 |
| Hpy188III | TCNNGA | 2 cut(s) | 212, 329 |
| HpyCH4V | TGCA | 3 cut(s) | 77, 121, 128 |
| HpyF3I | CTNAG | 2 cut(s) | 171, 185 |
| Hsp92II | CATG | 1 cut(s) | 83 |
| Kzo9I | GATC | 2 cut(s) | 289, 333 |
| LguI | GCTCTTC | 1 cut(s) | 87 |
| LmnI | GCTCC | 1 cut(s) | 265 |
| LpnPI | CCDG | 3 cut(s) | 29, 151, 187 |
| LweI | GCATC | 1 cut(s) | 205 |
| MaeI | CTAG | 3 cut(s) | 36, 48, 180 |
| MaeIII | GTNAC | 1 cut(s) | 64 |
| MalI | GATC | 2 cut(s) | 291, 335 |
| MboI | GATC | 2 cut(s) | 289, 333 |
| MboII | GAAGA | 1 cut(s) | 104 |
| MhlI | GDGCHC | 1 cut(s) | 138 |
| MluCI | AATT | 2 cut(s) | 269, 377 |
| MnlI | CCTC | 3 cut(s) | 46, 258, 278 |
| MslI | CAYNNNNRTG | 1 cut(s) | 150 |
| NdeII | GATC | 2 cut(s) | 289, 333 |
| NlaIII | CATG | 1 cut(s) | 83 |
| NlaIV | GGNNCC | 1 cut(s) | 109 |
| NspI | RCATGY | 1 cut(s) | 83 |
| PciSI | GCTCTTC | 1 cut(s) | 87 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 298 |
| PspN4I | GGNNCC | 1 cut(s) | 109 |
| RsaI | GTAC | 1 cut(s) | 22 |
| RsaNI | GTAC | 1 cut(s) | 21 |
| RseI | CAYNNNNRTG | 1 cut(s) | 150 |
| SapI | GCTCTTC | 1 cut(s) | 87 |
| Sau3AI | GATC | 2 cut(s) | 289, 333 |
| ScaI | AGTACT | 1 cut(s) | 22 |
| SduI | GDGCHC | 1 cut(s) | 138 |
| SetI | ASST | 6 cut(s) | 57, 75, 181, 289, 325, 359 |
| SfaNI | GCATC | 1 cut(s) | 205 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 150 |
| Sse9I | AATT | 2 cut(s) | 269, 377 |
| SspMI | CTAG | 3 cut(s) | 36, 48, 180 |
| StyI | CCWWGG | 2 cut(s) | 111, 179 |
| TaqI | TCGA | 2 cut(s) | 150, 292 |
| TasI | AATT | 2 cut(s) | 269, 377 |
| TatI | WGTACW | 1 cut(s) | 20 |
| TscAI | CASTG | 1 cut(s) | 109 |
| TspDTI | ATGAA | 2 cut(s) | 105, 357 |
| TspRI | CASTG | 1 cut(s) | 109 |
| XceI | RCATGY | 1 cut(s) | 83 |
| XmaJI | CCTAGG | 1 cut(s) | 179 |
| XspI | CTAG | 3 cut(s) | 36, 48, 180 |
| ZrmI | AGTACT | 1 cut(s) | 22 |