Glutathione S-transferase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1154 |
| Acc65I | GGTACC | 1 cut(s) | 266 |
| AccB1I | GGYRCC | 1 cut(s) | 266 |
| AccBSI | CCGCTC | 1 cut(s) | 562 |
| AciI | CCGC | 4 cut(s) | 230, 560, 1079, 1098 |
| AclWI | GGATC | 2 cut(s) | 853, 1139 |
| AcoI | YGGCCR | 1 cut(s) | 67 |
| AcsI | RAATTY | 2 cut(s) | 160, 426 |
| AcuI | CTGAAG | 2 cut(s) | 432, 848 |
| AdeI | CACNNNGTG | 1 cut(s) | 503 |
| AfaI | GTAC | 2 cut(s) | 268, 926 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 565, 566, 1159 |
| AgsI | TTSAA | 2 cut(s) | 373, 1110 |
| AjnI | CCWGG | 2 cut(s) | 564, 1158 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 42, 74 |
| Alw21I | GWGCWC | 4 cut(s) | 243, 812, 959, 1128 |
| Alw26I | GTCTC | 2 cut(s) | 138, 341 |
| AlwI | GGATC | 2 cut(s) | 853, 1139 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 821, 824 |
| AoxI | GGCC | 3 cut(s) | 67, 136, 354 |
| ApeKI | GCWGC | 4 cut(s) | 227, 815, 818, 821 |
| ApoI | RAATTY | 2 cut(s) | 160, 426 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 19, 51 |
| AseI | ATTAAT | 1 cut(s) | 540 |
| Asp718I | GGTACC | 1 cut(s) | 266 |
| AspS9I | GGNCC | 1 cut(s) | 988 |
| AsuHPI | GGTGA | 3 cut(s) | 283, 656, 878 |
| AsuII | TTCGAA | 1 cut(s) | 157 |
| AvaII | GGWCC | 1 cut(s) | 988 |
| BalI | TGGCCA | 1 cut(s) | 69 |
| BanI | GGYRCC | 1 cut(s) | 266 |
| BanII | GRGCYC | 2 cut(s) | 243, 812 |
| BbsI | GAAGAC | 1 cut(s) | 735 |
| Bbv12I | GWGCWC | 4 cut(s) | 243, 812, 959, 1128 |
| BbvI | GCAGC | 4 cut(s) | 214, 808, 827, 830 |
| BccI | CCATC | 5 cut(s) | 9, 82, 156, 307, 956 |
| BciT130I | CCWGG | 2 cut(s) | 566, 1160 |
| BclI | TGATCA | 2 cut(s) | 85, 1000 |
| BcoDI | GTCTC | 2 cut(s) | 138, 341 |
| BfaI | CTAG | 3 cut(s) | 176, 515, 1154 |
| BfmI | CTRYAG | 2 cut(s) | 549, 813 |
| BisI | GCNGC | 5 cut(s) | 228, 231, 816, 819, 822 |
| BlpI | GCTNAGC | 1 cut(s) | 835 |
| BlsI | GCNGC | 5 cut(s) | 229, 232, 817, 820, 823 |
| Bme1390I | CCNGG | 2 cut(s) | 566, 1160 |
| Bme18I | GGWCC | 1 cut(s) | 988 |
| BmgT120I | GGNCC | 1 cut(s) | 988 |
| BmiI | GGNNCC | 1 cut(s) | 268 |
| BmrFI | CCNGG | 2 cut(s) | 566, 1160 |
| BmrI | ACTGGG | 2 cut(s) | 20, 1078 |
| BmsI | GCATC | 6 cut(s) | 9, 31, 242, 501, 817, 1132 |
| BmuI | ACTGGG | 2 cut(s) | 20, 1078 |
| BpiI | GAAGAC | 1 cut(s) | 735 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 206, 238, 622, 654, 681, 713 |
| BpmI | CTGGAG | 2 cut(s) | 548, 708 |
| Bpu10I | CCTNAGC | 2 cut(s) | 186, 417 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 835 |
| Bpu14I | TTCGAA | 1 cut(s) | 157 |
| BsaI | GGTCTC | 1 cut(s) | 138 |
| BsaJI | CCNNGG | 3 cut(s) | 247, 565, 976 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 972, 1002 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 565, 566, 1159 |
| Bse1I | ACTGG | 3 cut(s) | 15, 1084, 1133 |
| Bse3DI | GCAATG | 2 cut(s) | 439, 538 |
| BseBI | CCWGG | 2 cut(s) | 566, 1160 |
| BseDI | CCNNGG | 3 cut(s) | 247, 565, 976 |
| BseGI | GGATG | 3 cut(s) | 20, 24, 427 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 565, 566, 1159 |
| BseMI | GCAATG | 2 cut(s) | 439, 538 |
| BseMII | CTCAG | 5 cut(s) | 228, 291, 491, 712, 849 |
| BseNI | ACTGG | 3 cut(s) | 15, 1084, 1133 |
| BseRI | GAGGAG | 1 cut(s) | 722 |
| BseXI | GCAGC | 4 cut(s) | 214, 808, 827, 830 |
| BseYI | CCCAGC | 1 cut(s) | 905 |
| BshFI | GGCC | 3 cut(s) | 69, 138, 356 |
| BshNI | GGYRCC | 1 cut(s) | 266 |
| BsiHKAI | GWGCWC | 4 cut(s) | 243, 812, 959, 1128 |
| BslFI | GGGAC | 1 cut(s) | 506 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 565, 566, 1159 |
| BsmAI | GTCTC | 2 cut(s) | 138, 341 |
| BsmBI | CGTCTC | 1 cut(s) | 341 |
| BsmFI | GGGAC | 1 cut(s) | 506 |
| BsmI | GAATGC | 2 cut(s) | 799, 907 |
| BsnI | GGCC | 3 cut(s) | 69, 138, 356 |
| Bso31I | GGTCTC | 1 cut(s) | 138 |
| Bsp119I | TTCGAA | 1 cut(s) | 157 |
| Bsp1286I | GDGCHC | 4 cut(s) | 243, 812, 959, 1128 |
| Bsp143I | GATC | 7 cut(s) | 85, 193, 721, 858, 982, 1000, 1131 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 835 |
| Bsp19I | CCATGG | 2 cut(s) | 247, 976 |
| BspACI | CCGC | 4 cut(s) | 230, 560, 1079, 1098 |
| BspANI | GGCC | 3 cut(s) | 69, 138, 356 |
| BspCNI | CTCAG | 5 cut(s) | 229, 290, 492, 711, 848 |
| BspHI | TCATGA | 1 cut(s) | 771 |
| BspLI | GGNNCC | 1 cut(s) | 268 |
| BspMAI | CTGCAG | 1 cut(s) | 817 |
| BspPI | GGATC | 2 cut(s) | 853, 1139 |
| BspQI | GCTCTTC | 1 cut(s) | 248 |
| BspT104I | TTCGAA | 1 cut(s) | 157 |
| BspT107I | GGYRCC | 1 cut(s) | 266 |
| BspTNI | GGTCTC | 1 cut(s) | 138 |
| BsrBI | CCGCTC | 1 cut(s) | 562 |
| BsrDI | GCAATG | 2 cut(s) | 439, 538 |
| BsrI | ACTGG | 3 cut(s) | 15, 1084, 1133 |
| BssECI | CCNNGG | 3 cut(s) | 247, 565, 976 |
| BssMI | GATC | 7 cut(s) | 85, 193, 721, 858, 982, 1000, 1131 |
| BssT1I | CCWWGG | 2 cut(s) | 247, 976 |
| Bst2UI | CCWGG | 2 cut(s) | 566, 1160 |
| Bst4CI | ACNGT | 2 cut(s) | 553, 613 |
| Bst6I | CTCTTC | 4 cut(s) | 207, 248, 653, 669 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 821 |
| BstBI | TTCGAA | 1 cut(s) | 157 |
| BstC8I | GCNNGC | 6 cut(s) | 71, 560, 717, 832, 1052, 1100 |
| BstDEI | CTNAG | 7 cut(s) | 186, 237, 277, 417, 500, 698, 835 |
| BstDSI | CCRYGG | 2 cut(s) | 247, 976 |
| BstF5I | GGATG | 3 cut(s) | 20, 24, 427 |
| BstKTI | GATC | 7 cut(s) | 88, 196, 724, 861, 985, 1003, 1134 |
| BstMAI | GTCTC | 2 cut(s) | 138, 341 |
| BstMBI | GATC | 7 cut(s) | 85, 193, 721, 858, 982, 1000, 1131 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 462, 821, 827, 836, 1056, 1065 |
| BstNI | CCWGG | 2 cut(s) | 566, 1160 |
| BstNSI | RCATGY | 1 cut(s) | 1054 |
| BstSCI | CCNGG | 2 cut(s) | 564, 1158 |
| BstSFI | CTRYAG | 2 cut(s) | 549, 813 |
| BstV1I | GCAGC | 4 cut(s) | 214, 808, 827, 830 |
| BstV2I | GAAGAC | 1 cut(s) | 735 |
| BstX2I | RGATCY | 1 cut(s) | 858 |
| BstYI | RGATCY | 1 cut(s) | 858 |
| BsuRI | GGCC | 3 cut(s) | 69, 138, 356 |
| BtgI | CCRYGG | 2 cut(s) | 247, 976 |
| BtsCI | GGATG | 3 cut(s) | 20, 24, 427 |
| BtsI | GCAGTG | 1 cut(s) | 311 |
| BtsIMutI | CAGTG | 4 cut(s) | 311, 497, 957, 1126 |
| Cac8I | GCNNGC | 6 cut(s) | 71, 560, 717, 832, 1052, 1100 |
| CaiI | CAGNNNCTG | 2 cut(s) | 821, 824 |
| CciI | TCATGA | 1 cut(s) | 771 |
| Cfr13I | GGNCC | 1 cut(s) | 988 |
| CseI | GACGC | 1 cut(s) | 1065 |
| Csp6I | GTAC | 2 cut(s) | 267, 925 |
| CviAII | CATG | 8 cut(s) | 248, 458, 482, 772, 790, 977, 1012, 1051 |
| CviQI | GTAC | 2 cut(s) | 267, 925 |
| DdeI | CTNAG | 7 cut(s) | 186, 237, 277, 417, 500, 698, 835 |
| DpnI | GATC | 7 cut(s) | 87, 195, 723, 860, 984, 1002, 1133 |
| DpnII | GATC | 7 cut(s) | 85, 193, 721, 858, 982, 1000, 1131 |
| DraIII | CACNNNGTG | 1 cut(s) | 503 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1154 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1154 |
| EaeI | YGGCCR | 1 cut(s) | 67 |
| Eam1104I | CTCTTC | 4 cut(s) | 207, 248, 653, 669 |
| EarI | CTCTTC | 4 cut(s) | 207, 248, 653, 669 |
| Ecl136II | GAGCTC | 2 cut(s) | 241, 810 |
| Eco130I | CCWWGG | 2 cut(s) | 247, 976 |
| Eco24I | GRGCYC | 2 cut(s) | 243, 812 |
| Eco31I | GGTCTC | 1 cut(s) | 138 |
| Eco32I | GATATC | 1 cut(s) | 572 |
| Eco47I | GGWCC | 1 cut(s) | 988 |
| Eco53kI | GAGCTC | 2 cut(s) | 241, 810 |
| Eco57I | CTGAAG | 2 cut(s) | 432, 848 |
| EcoICRI | GAGCTC | 2 cut(s) | 241, 810 |
| EcoO109I | RGGNCCY | 1 cut(s) | 988 |
| EcoRII | CCWGG | 2 cut(s) | 564, 1158 |
| EcoRV | GATATC | 1 cut(s) | 572 |
| EcoT14I | CCWWGG | 2 cut(s) | 247, 976 |
| EcoT22I | ATGCAT | 1 cut(s) | 24 |
| EcoT38I | GRGCYC | 2 cut(s) | 243, 812 |
| ErhI | CCWWGG | 2 cut(s) | 247, 976 |
| Esp3I | CGTCTC | 1 cut(s) | 341 |
| FaeI | CATG | 8 cut(s) | 251, 461, 485, 775, 793, 980, 1015, 1054 |
| FaqI | GGGAC | 1 cut(s) | 506 |
| FatI | CATG | 8 cut(s) | 247, 457, 481, 771, 789, 976, 1011, 1050 |
| FauI | CCCGC | 3 cut(s) | 567, 1086, 1091 |
| FauNDI | CATATG | 1 cut(s) | 589 |
| FbaI | TGATCA | 2 cut(s) | 85, 1000 |
| Fnu4HI | GCNGC | 5 cut(s) | 228, 231, 816, 819, 822 |
| FokI | GGATG | 3 cut(s) | 27, 31, 434 |
| FriOI | GRGCYC | 2 cut(s) | 243, 812 |
| Fsp4HI | GCNGC | 5 cut(s) | 228, 231, 816, 819, 822 |
| FspBI | CTAG | 3 cut(s) | 176, 515, 1154 |
| GluI | GCNGC | 5 cut(s) | 228, 231, 816, 819, 822 |
| GsaI | CCCAGC | 1 cut(s) | 909 |
| GsuI | CTGGAG | 2 cut(s) | 548, 708 |
| HaeIII | GGCC | 3 cut(s) | 69, 138, 356 |
| HgaI | GACGC | 1 cut(s) | 1065 |
| Hin1II | CATG | 8 cut(s) | 251, 461, 485, 775, 793, 980, 1015, 1054 |
| HinfI | GANTC | 4 cut(s) | 179, 218, 437, 1106 |
| HphI | GGTGA | 3 cut(s) | 283, 656, 878 |
| Hpy166II | GTNNAC | 1 cut(s) | 886 |
| Hpy188I | TCNGA | 8 cut(s) | 39, 238, 280, 601, 657, 726, 807, 867 |
| Hpy188III | TCNNGA | 8 cut(s) | 176, 197, 328, 444, 687, 772, 1004, 1022 |
| Hpy8I | GTNNAC | 1 cut(s) | 886 |
| Hpy99I | CGWCG | 1 cut(s) | 1059 |
| HpyAV | CCTTC | 5 cut(s) | 391, 441, 616, 721, 872 |
| HpyCH4III | ACNGT | 2 cut(s) | 553, 613 |
| HpyCH4V | TGCA | 7 cut(s) | 22, 260, 432, 815, 972, 1091, 1145 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 462, 821, 827, 836, 1056, 1065 |
| HpyF3I | CTNAG | 7 cut(s) | 186, 237, 277, 417, 500, 698, 835 |
| Hsp92II | CATG | 8 cut(s) | 251, 461, 485, 775, 793, 980, 1015, 1054 |
| KpnI | GGTACC | 1 cut(s) | 270 |
| Ksp22I | TGATCA | 2 cut(s) | 85, 1000 |
| Kzo9I | GATC | 7 cut(s) | 85, 193, 721, 858, 982, 1000, 1131 |
| LguI | GCTCTTC | 1 cut(s) | 248 |
| LmnI | GCTCC | 6 cut(s) | 567, 625, 689, 807, 1123, 1162 |
| Lsp1109I | GCAGC | 4 cut(s) | 214, 808, 827, 830 |
| LweI | GCATC | 6 cut(s) | 9, 31, 242, 501, 817, 1132 |
| MaeI | CTAG | 3 cut(s) | 176, 515, 1154 |
| MaeIII | GTNAC | 1 cut(s) | 6 |
| MalI | GATC | 7 cut(s) | 87, 195, 723, 860, 984, 1002, 1133 |
| MbiI | CCGCTC | 1 cut(s) | 562 |
| MboI | GATC | 7 cut(s) | 85, 193, 721, 858, 982, 1000, 1131 |
| MfeI | CAATTG | 1 cut(s) | 261 |
| MflI | RGATCY | 1 cut(s) | 858 |
| MhlI | GDGCHC | 4 cut(s) | 243, 812, 959, 1128 |
| MlsI | TGGCCA | 1 cut(s) | 69 |
| MluCI | AATT | 6 cut(s) | 160, 261, 368, 426, 665, 679 |
| MluNI | TGGCCA | 1 cut(s) | 69 |
| MmeI | TCCRAC | 1 cut(s) | 372 |
| MnlI | CCTC | 7 cut(s) | 71, 208, 232, 346, 624, 700, 1001 |
| Mox20I | TGGCCA | 1 cut(s) | 69 |
| Mph1103I | ATGCAT | 1 cut(s) | 24 |
| MscI | TGGCCA | 1 cut(s) | 69 |
| MseI | TTAA | 2 cut(s) | 129, 540 |
| MslI | CAYNNNNRTG | 1 cut(s) | 792 |
| Msp20I | TGGCCA | 1 cut(s) | 69 |
| MspA1I | CMGCKG | 1 cut(s) | 821 |
| MspR9I | CCNGG | 2 cut(s) | 566, 1160 |
| MunI | CAATTG | 1 cut(s) | 261 |
| Mva1269I | GAATGC | 2 cut(s) | 799, 907 |
| MvaI | CCWGG | 2 cut(s) | 566, 1160 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 462, 821, 827, 836, 1056, 1065 |
| NcoI | CCATGG | 2 cut(s) | 247, 976 |
| NdeI | CATATG | 1 cut(s) | 589 |
| NdeII | GATC | 7 cut(s) | 85, 193, 721, 858, 982, 1000, 1131 |
| NlaIII | CATG | 8 cut(s) | 251, 461, 485, 775, 793, 980, 1015, 1054 |
| NlaIV | GGNNCC | 1 cut(s) | 268 |
| NsiI | ATGCAT | 1 cut(s) | 24 |
| NspI | RCATGY | 1 cut(s) | 1054 |
| NspV | TTCGAA | 1 cut(s) | 157 |
| PaeI | GCATGC | 1 cut(s) | 1054 |
| PagI | TCATGA | 1 cut(s) | 771 |
| PciSI | GCTCTTC | 1 cut(s) | 248 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 31 |
| PctI | GAATGC | 2 cut(s) | 799, 907 |
| PfeI | GAWTC | 4 cut(s) | 179, 218, 437, 1106 |
| PflFI | GACNNNGTC | 1 cut(s) | 284 |
| PfoI | TCCNGGA | 1 cut(s) | 1158 |
| PkrI | GCNGC | 5 cut(s) | 229, 232, 817, 820, 823 |
| PpuMI | RGGWCCY | 1 cut(s) | 988 |
| PshBI | ATTAAT | 1 cut(s) | 540 |
| Psp124BI | GAGCTC | 2 cut(s) | 243, 812 |
| Psp5II | RGGWCCY | 1 cut(s) | 988 |
| Psp6I | CCWGG | 2 cut(s) | 564, 1158 |
| PspFI | CCCAGC | 1 cut(s) | 905 |
| PspGI | CCWGG | 2 cut(s) | 564, 1158 |
| PspN4I | GGNNCC | 1 cut(s) | 268 |
| PspPI | GGNCC | 1 cut(s) | 988 |
| PspPPI | RGGWCCY | 1 cut(s) | 988 |
| PstI | CTGCAG | 1 cut(s) | 817 |
| PstNI | CAGNNNCTG | 2 cut(s) | 821, 824 |
| PsuI | RGATCY | 1 cut(s) | 858 |
| PsyI | GACNNNGTC | 1 cut(s) | 284 |
| PvuII | CAGCTG | 1 cut(s) | 821 |
| RsaI | GTAC | 2 cut(s) | 268, 926 |
| RsaNI | GTAC | 2 cut(s) | 267, 925 |
| RseI | CAYNNNNRTG | 1 cut(s) | 792 |
| SacI | GAGCTC | 2 cut(s) | 243, 812 |
| SapI | GCTCTTC | 1 cut(s) | 248 |
| SaqAI | TTAA | 2 cut(s) | 129, 540 |
| SatI | GCNGC | 5 cut(s) | 228, 231, 816, 819, 822 |
| Sau3AI | GATC | 7 cut(s) | 85, 193, 721, 858, 982, 1000, 1131 |
| Sau96I | GGNCC | 1 cut(s) | 988 |
| ScrFI | CCNGG | 2 cut(s) | 566, 1160 |
| SduI | GDGCHC | 4 cut(s) | 243, 812, 959, 1128 |
| SfaNI | GCATC | 6 cut(s) | 9, 31, 242, 501, 817, 1132 |
| SfcI | CTRYAG | 2 cut(s) | 549, 813 |
| SfuI | TTCGAA | 1 cut(s) | 157 |
| SinI | GGWCC | 1 cut(s) | 988 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 792 |
| SphI | GCATGC | 1 cut(s) | 1054 |
| Sse9I | AATT | 6 cut(s) | 160, 261, 368, 426, 665, 679 |
| SsiI | CCGC | 4 cut(s) | 230, 560, 1079, 1098 |
| SspMI | CTAG | 3 cut(s) | 176, 515, 1154 |
| SstI | GAGCTC | 2 cut(s) | 243, 812 |
| StyD4I | CCNGG | 2 cut(s) | 564, 1158 |
| StyI | CCWWGG | 2 cut(s) | 247, 976 |
| TaaI | ACNGT | 2 cut(s) | 553, 613 |
| TaqI | TCGA | 5 cut(s) | 25, 81, 157, 327, 899 |
| TaqII | GACCGA | 1 cut(s) | 686 |
| TasI | AATT | 6 cut(s) | 160, 261, 368, 426, 665, 679 |
| TauI | GCSGC | 1 cut(s) | 233 |
| TfiI | GAWTC | 4 cut(s) | 179, 218, 437, 1106 |
| Tru1I | TTAA | 2 cut(s) | 129, 540 |
| Tru9I | TTAA | 2 cut(s) | 129, 540 |
| TscAI | CASTG | 4 cut(s) | 318, 504, 964, 1133 |
| TseI | GCWGC | 4 cut(s) | 227, 815, 818, 821 |
| TspGWI | ACGGA | 2 cut(s) | 365, 483 |
| TspRI | CASTG | 4 cut(s) | 318, 504, 964, 1133 |
| Tth111I | GACNNNGTC | 1 cut(s) | 284 |
| VpaK11BI | GGWCC | 1 cut(s) | 988 |
| VspI | ATTAAT | 1 cut(s) | 540 |
| XapI | RAATTY | 2 cut(s) | 160, 426 |
| XbaI | TCTAGA | 1 cut(s) | 175 |
| XceI | RCATGY | 1 cut(s) | 1054 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1091 |
| XspI | CTAG | 3 cut(s) | 176, 515, 1154 |
| Zsp2I | ATGCAT | 1 cut(s) | 24 |