Rmu_sc0000528.1_g000009

Zinc knuckle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000528.1
OG0000336
Physical Location & Seq
Forward (+)
43476 .. 44072
597 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000528.1_g000009.1.cds

Sequence Viewer

Length: 468 bp
atggaggacgtcaacaagttggcagtgcgcctggctacatccttgggactggtggatgcgcgccgatcggtggacttgcaaccttctcgtggtgaaggattacatcaatcaaaaacgtaccttgtggggaaacttctgacagccaaagctttcaacaaaagatcttttatgggaatagttctggtggttttggcaccatatgacagagttggagcaatcaaagatgtgtcgcttgctacactagctttctggatcaaggtgtgtggactactgccggattttcacatggatcgctgtcttaggaggattggcagtgccataggtcgatttgttcaaaaggacaccacagagtttgatgcaggaagaattcggattttggttgagttggacctgctgcagcctatcatctttcgatgtagcttcacggtagatgatgttattgtggtggatttagaattcttcttctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

155

Amino Acids

17.4

Weight (kDa)

8.88

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000336)

Species Orthologous Gene IDs
malus_domestica MD05G1024100.v1.1
rosa_chinensis RchiOBHm_Chr2g0152291 RchiOBHm_Chr4g0387411
rosa_laevigata RLG00000009698 RLG00000009699 RLG00000009700 RLG00000023615 RLG00000024265 RLG00000034445 RLG00000036567
rosa_multiflora Rmu_co8268067.1_g000001 Rmu_co8313555.1_g000001 Rmu_co8324697.1_g000001 Rmu_sc0000318.1_g000023 Rmu_sc0000342.1_g000016 Rmu_sc0000447.1_g000010 Rmu_sc0000528.1_g000009 Rmu_sc0000539.1_g000060 Rmu_sc0000929.1_g000023 Rmu_sc0001493.1_g000081 Rmu_sc0001674.1_g000003 Rmu_sc0001791.1_g000023 Rmu_sc0001822.1_g000005 Rmu_sc0001822.1_g000033 Rmu_sc0002061.1_g000010 Rmu_sc0002286.1_g000012 Rmu_sc0002979.1_g000009 Rmu_sc0003158.1_g000016 Rmu_sc0003221.1_g000001 Rmu_sc0003641.1_g000029 Rmu_sc0003742.1_g000012 Rmu_sc0003814.1_g000004 Rmu_sc0003823.1_g000005 Rmu_sc0004002.1_g000005 Rmu_sc0004564.1_g000005 Rmu_sc0005415.1_g000003 Rmu_sc0006050.1_g000025 Rmu_sc0006555.1_g000002 Rmu_sc0009954.1_g000020 Rmu_sc0010421.1_g000007 Rmu_sc0010607.1_g000002 Rmu_sc0011044.1_g000005 Rmu_sc0011497.1_g000015 Rmu_sc0014532.1_g000004 Rmu_sc0014745.1_g000005 Rmu_sc0014821.1_g000001 Rmu_sc0015038.1_g000002 Rmu_sc0015454.1_g000004 Rmu_sc0019663.1_g000002 Rmu_sc0027475.1_g000003 Rmu_sc0028826.1_g000002 Rmu_ssc0000117.1_g000019 Rmu_ssc0000175.1_g000034
rosa_roxburghii Rroxscaffold_3G00237080 Rroxscaffold_4G00288290 Rroxscaffold_6G00397510
rosa_rugosa Rorug01G0035000 Rorug01G0190900 Rorug01G0208500 Rorug01G0208600 Rorug01G0208600 Rorug01G0344900 Rorug01G0479500 Rorug01G0479600 Rorug02G0230400 Rorug02G0320900 Rorug02G0433300 Rorug03G0147400 Rorug03G0169700.1 Rorug03G0169800 Rorug03G0198300 Rorug04G0022900 Rorug04G0064600 Rorug04G0322100 Rorug04G0388900 Rorug05G0153200 Rorug05G0231600 Rorug05G0231700 Rorug05G0231800 Rorug05G0276200 Rorug05G0292400 Rorug05G0314700 Rorug06G0092000 Rorug06G0260900 Rorug06G0273600 RorugPtG0003200
rosa_samantha Rh1AG264100 Rh1AG292500 Rh1AG423800 Rh2AG098000 Rh2AG232700 Rh2AG361100 Rh2AG372900 Rh2BG099200 Rh2BG246000 Rh2BG260600 Rh2BG378400 Rh3BG142300 Rh3BG252900 Rh3BG267700 Rh3DG302400 Rh5BG059800 Rh5BG320000 Rh5BG530500 Rh6AG089300 Rh6AG089400 Rh6AG127400 Rh6AG376700 Rh7CG488700 Rh7DG265900
rosa_wichuraiana Rw2G040640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 12
Acc36I ACCTGC 1 cut(s) 399
AccB1I GGYRCC 1 cut(s) 193
AccII CGCG 1 cut(s) 61
AclWI GGATC 2 cut(s) 260, 297
AcsI RAATTY 2 cut(s) 366, 455
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 1 cut(s) 119
AfiI CCNNNNNNNGG 2 cut(s) 70, 89
AgsI TTSAA 2 cut(s) 154, 335
AjnI CCWGG 1 cut(s) 30
AluBI AGCT 3 cut(s) 149, 245, 420
AluI AGCT 3 cut(s) 149, 245, 420
AlwI GGATC 2 cut(s) 260, 297
ApeKI GCWGC 2 cut(s) 394, 397
ApoI RAATTY 2 cut(s) 366, 455
AspLEI GCGC 3 cut(s) 30, 61, 63
AspS9I GGNCC 1 cut(s) 388
AsuHPI GGTGA 1 cut(s) 104
AvaII GGWCC 1 cut(s) 388
BanI GGYRCC 1 cut(s) 193
BauI CACGAG 1 cut(s) 87
BbvI GCAGC 2 cut(s) 381, 409
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 1 cut(s) 32
BfaI CTAG 2 cut(s) 242, 466
BfmI CTRYAG 1 cut(s) 395
BfuAI ACCTGC 1 cut(s) 399
BglII AGATCT 1 cut(s) 161
BisI GCNGC 2 cut(s) 395, 398
BlsI GCNGC 2 cut(s) 396, 399
Bme1390I CCNGG 1 cut(s) 32
Bme18I GGWCC 1 cut(s) 388
BmgT120I GGNCC 1 cut(s) 388
BmiI GGNNCC 1 cut(s) 195
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 2 cut(s) 46, 346
BsaHI GRCGYC 1 cut(s) 9
BsaJI CCNNGG 1 cut(s) 42
Bsc4I CCNNNNNNNGG 2 cut(s) 70, 89
Bse1I ACTGG 1 cut(s) 54
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 1 cut(s) 42
BseGI GGATG 2 cut(s) 38, 61
BseLI CCNNNNNNNGG 2 cut(s) 70, 89
BseNI ACTGG 1 cut(s) 54
BsePI GCGCGC 1 cut(s) 59
BseXI GCAGC 2 cut(s) 381, 409
Bsh1236I CGCG 1 cut(s) 61
Bsh1285I CGRYCG 1 cut(s) 68
BshNI GGYRCC 1 cut(s) 193
BsiEI CGRYCG 1 cut(s) 68
BsiSI CCGG 1 cut(s) 275
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 70, 89
BsmFI GGGAC 1 cut(s) 60
Bsp143I GATC 4 cut(s) 65, 161, 252, 289
BspFNI CGCG 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 195
BspMAI CTGCAG 1 cut(s) 399
BspMI ACCTGC 1 cut(s) 399
BspPI GGATC 2 cut(s) 260, 297
BspT107I GGYRCC 1 cut(s) 193
BsrI ACTGG 1 cut(s) 54
BssECI CCNNGG 1 cut(s) 42
BssHII GCGCGC 1 cut(s) 59
BssMI GATC 4 cut(s) 65, 161, 252, 289
BssNI GRCGYC 1 cut(s) 9
BssSI CACGAG 1 cut(s) 87
BssT1I CCWWGG 1 cut(s) 42
Bst2BI CACGAG 1 cut(s) 87
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 1 cut(s) 427
BstACI GRCGYC 1 cut(s) 9
BstC8I GCNNGC 2 cut(s) 61, 234
BstDEI CTNAG 1 cut(s) 299
BstF5I GGATG 2 cut(s) 38, 61
BstFNI CGCG 1 cut(s) 61
BstHHI GCGC 3 cut(s) 30, 61, 63
BstKTI GATC 4 cut(s) 68, 164, 255, 292
BstMBI GATC 4 cut(s) 65, 161, 252, 289
BstMCI CGRYCG 1 cut(s) 68
BstMWI GCNNNNNNNGC 1 cut(s) 242
BstNI CCWGG 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 30
BstSFI CTRYAG 1 cut(s) 395
BstUI CGCG 1 cut(s) 61
BstV1I GCAGC 2 cut(s) 381, 409
BstX2I RGATCY 1 cut(s) 161
BstYI RGATCY 1 cut(s) 161
BtsCI GGATG 2 cut(s) 38, 61
BtsI GCAGTG 2 cut(s) 30, 319
BtsIMutI CAGTG 2 cut(s) 30, 319
BveI ACCTGC 1 cut(s) 399
Cac8I GCNNGC 2 cut(s) 61, 234
CfoI GCGC 3 cut(s) 30, 61, 63
Cfr13I GGNCC 1 cut(s) 388
Csp6I GTAC 1 cut(s) 118
CspCI CAANNNNNGTGG 4 cut(s) 244, 279, 334, 369
CviAII CATG 1 cut(s) 286
CviJI RGCY 6 cut(s) 35, 143, 149, 245, 400, 420
CviKI_1 RGCY 6 cut(s) 35, 143, 149, 245, 400, 420
CviQI GTAC 1 cut(s) 118
DdeI CTNAG 1 cut(s) 299
DpnI GATC 4 cut(s) 67, 163, 254, 291
DpnII GATC 4 cut(s) 65, 161, 252, 289
Eco130I CCWWGG 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 388
EcoRI GAATTC 2 cut(s) 366, 455
EcoRII CCWGG 1 cut(s) 30
EcoT14I CCWWGG 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 1 cut(s) 289
FaiI YATR 5 cut(s) 170, 199, 201, 287, 320
FaqI GGGAC 1 cut(s) 60
FatI CATG 1 cut(s) 285
FauNDI CATATG 1 cut(s) 199
Fnu4HI GCNGC 2 cut(s) 395, 398
FokI GGATG 2 cut(s) 25, 68
Fsp4HI GCNGC 2 cut(s) 395, 398
FspBI CTAG 2 cut(s) 242, 466
GlaI GCGC 3 cut(s) 29, 60, 62
GluI GCNGC 2 cut(s) 395, 398
HapII CCGG 1 cut(s) 275
HhaI GCGC 3 cut(s) 30, 61, 63
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 1 cut(s) 289
Hin6I GCGC 3 cut(s) 28, 59, 61
HinP1I GCGC 3 cut(s) 28, 59, 61
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HindIII AAGCTT 1 cut(s) 147
HpaII CCGG 1 cut(s) 275
HphI GGTGA 1 cut(s) 104
Hpy166II GTNNAC 3 cut(s) 13, 73, 266
Hpy188I TCNGA 2 cut(s) 138, 372
Hpy188III TCNNGA 1 cut(s) 250
Hpy8I GTNNAC 3 cut(s) 13, 73, 266
HpyAV CCTTC 2 cut(s) 89, 93
HpyCH4III ACNGT 1 cut(s) 427
HpyCH4IV ACGT 2 cut(s) 9, 116
HpyCH4V TGCA 3 cut(s) 79, 359, 397
HpyF10VI GCNNNNNNNGC 1 cut(s) 242
HpyF3I CTNAG 1 cut(s) 299
HpySE526I ACGT 2 cut(s) 9, 116
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 1 cut(s) 289
HspAI GCGC 3 cut(s) 28, 59, 61
Kzo9I GATC 4 cut(s) 65, 161, 252, 289
LmnI GCTCC 1 cut(s) 212
LpnPI CCDG 8 cut(s) 17, 35, 44, 167, 235, 288, 345, 404
Lsp1109I GCAGC 2 cut(s) 381, 409
LweI GCATC 2 cut(s) 46, 346
MaeI CTAG 2 cut(s) 242, 466
MaeII ACGT 2 cut(s) 9, 116
MalI GATC 4 cut(s) 67, 163, 254, 291
MboI GATC 4 cut(s) 65, 161, 252, 289
MboII GAAGA 3 cut(s) 375, 451, 454
MflI RGATCY 1 cut(s) 161
MluCI AATT 2 cut(s) 366, 455
MmeI TCCRAC 2 cut(s) 190, 366
MnlI CCTC 1 cut(s) 297
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 1 cut(s) 32
MvaI CCWGG 1 cut(s) 32
MvnI CGCG 1 cut(s) 61
MwoI GCNNNNNNNGC 1 cut(s) 242
NdeI CATATG 1 cut(s) 199
NdeII GATC 4 cut(s) 65, 161, 252, 289
NlaIII CATG 1 cut(s) 289
NlaIV GGNNCC 1 cut(s) 195
PauI GCGCGC 1 cut(s) 59
PkrI GCNGC 2 cut(s) 396, 399
Ple19I CGATCG 1 cut(s) 68
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 1 cut(s) 195
PspPI GGNCC 1 cut(s) 388
PstI CTGCAG 1 cut(s) 399
PsuI RGATCY 1 cut(s) 161
PteI GCGCGC 1 cut(s) 59
PvuI CGATCG 1 cut(s) 68
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
SatI GCNGC 2 cut(s) 395, 398
Sau3AI GATC 4 cut(s) 65, 161, 252, 289
Sau96I GGNCC 1 cut(s) 388
ScrFI CCNGG 1 cut(s) 32
SfaNI GCATC 2 cut(s) 46, 346
SfcI CTRYAG 1 cut(s) 395
SinI GGWCC 1 cut(s) 388
Sse9I AATT 2 cut(s) 366, 455
SspMI CTAG 2 cut(s) 242, 466
StyD4I CCNGG 1 cut(s) 30
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 1 cut(s) 427
TaiI ACGT 2 cut(s) 12, 119
TaqI TCGA 2 cut(s) 325, 412
TasI AATT 2 cut(s) 366, 455
TscAI CASTG 2 cut(s) 30, 319
TseI GCWGC 2 cut(s) 394, 397
TspRI CASTG 2 cut(s) 30, 319
VpaK11BI GGWCC 1 cut(s) 388
XapI RAATTY 2 cut(s) 366, 455
XspI CTAG 2 cut(s) 242, 466
ZraI GACGTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.