Rmu_sc0000539.1_g000060

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000539.1
OG0000336
Physical Location & Seq
Reverse (-)
282617 .. 284141
1525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000539.1_g000060.1.cds

Sequence Viewer

Length: 819 bp
atggtacctgaacttatcatctatcttatcgatgacattcacaggccggtggatcttcgtcttgctcgtggagagggtttgtgtcatgttcagacgtatctggtggcgaagttgttgacggctagggctttcaataaaagatcgttcatgggcatgttccggcatgcatggaggctgaatggacagtacactgctcagaaacatggagaccgctttctattcaccttaacggatccaacagaccgtaatcggatccttcgagttggtccttggggctttgatagagctccggtacgtggcctgccaccagattttcatactgatcgttgcttgagaaggattggtagcgctttagggcggtatgtgcagaaggacacaacagaatttgaagaaggaaggattcggatccgagttgagattgatcttctgcagcctgtgatttttcgaaggaatttcatggtggatgatggggttgatgtggatatggattttttcttcgaaaacctctatggaagatgtcaagaatgtggtttgattactcatgtgggattaccatgcgttgggacgccgttgtcggttgaggccaggactgaccaatcgccggtacgacgaaatcttgaaagggttttgaaccatgtggagttgtctcatcaggttggaccgtcgatggtttttggagcacagtcgagtccacaagtagggctagagagatttctgccagcagctaaatcaaatgggagaaaggaaattaggagtcgacttgtcaccggtagtggtgaagaaagttcaggggtgcacaatgcatctttgaacgagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4 zf-RING_2

Protein Analysis

272

Amino Acids

30.94

Weight (kDa)

8.89

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000336)

Species Orthologous Gene IDs
malus_domestica MD05G1024100.v1.1
rosa_chinensis RchiOBHm_Chr2g0152291 RchiOBHm_Chr4g0387411
rosa_laevigata RLG00000009698 RLG00000009699 RLG00000009700 RLG00000023615 RLG00000024265 RLG00000034445 RLG00000036567
rosa_multiflora Rmu_co8268067.1_g000001 Rmu_co8313555.1_g000001 Rmu_co8324697.1_g000001 Rmu_sc0000318.1_g000023 Rmu_sc0000342.1_g000016 Rmu_sc0000447.1_g000010 Rmu_sc0000528.1_g000009 Rmu_sc0000539.1_g000060 Rmu_sc0000929.1_g000023 Rmu_sc0001493.1_g000081 Rmu_sc0001674.1_g000003 Rmu_sc0001791.1_g000023 Rmu_sc0001822.1_g000005 Rmu_sc0001822.1_g000033 Rmu_sc0002061.1_g000010 Rmu_sc0002286.1_g000012 Rmu_sc0002979.1_g000009 Rmu_sc0003158.1_g000016 Rmu_sc0003221.1_g000001 Rmu_sc0003641.1_g000029 Rmu_sc0003742.1_g000012 Rmu_sc0003814.1_g000004 Rmu_sc0003823.1_g000005 Rmu_sc0004002.1_g000005 Rmu_sc0004564.1_g000005 Rmu_sc0005415.1_g000003 Rmu_sc0006050.1_g000025 Rmu_sc0006555.1_g000002 Rmu_sc0009954.1_g000020 Rmu_sc0010421.1_g000007 Rmu_sc0010607.1_g000002 Rmu_sc0011044.1_g000005 Rmu_sc0011497.1_g000015 Rmu_sc0014532.1_g000004 Rmu_sc0014745.1_g000005 Rmu_sc0014821.1_g000001 Rmu_sc0015038.1_g000002 Rmu_sc0015454.1_g000004 Rmu_sc0019663.1_g000002 Rmu_sc0027475.1_g000003 Rmu_sc0028826.1_g000002 Rmu_ssc0000117.1_g000019 Rmu_ssc0000175.1_g000034
rosa_roxburghii Rroxscaffold_3G00237080 Rroxscaffold_4G00288290 Rroxscaffold_6G00397510
rosa_rugosa Rorug01G0035000 Rorug01G0190900 Rorug01G0208500 Rorug01G0208600 Rorug01G0208600 Rorug01G0344900 Rorug01G0479500 Rorug01G0479600 Rorug02G0230400 Rorug02G0320900 Rorug02G0433300 Rorug03G0147400 Rorug03G0169700.1 Rorug03G0169800 Rorug03G0198300 Rorug04G0022900 Rorug04G0064600 Rorug04G0322100 Rorug04G0388900 Rorug05G0153200 Rorug05G0231600 Rorug05G0231700 Rorug05G0231800 Rorug05G0276200 Rorug05G0292400 Rorug05G0314700 Rorug06G0092000 Rorug06G0260900 Rorug06G0273600 RorugPtG0003200
rosa_samantha Rh1AG264100 Rh1AG292500 Rh1AG423800 Rh2AG098000 Rh2AG232700 Rh2AG361100 Rh2AG372900 Rh2BG099200 Rh2BG246000 Rh2BG260600 Rh2BG378400 Rh3BG142300 Rh3BG252900 Rh3BG267700 Rh3DG302400 Rh5BG059800 Rh5BG320000 Rh5BG530500 Rh6AG089300 Rh6AG089400 Rh6AG127400 Rh6AG376700 Rh7CG488700 Rh7DG265900
rosa_wichuraiana Rw2G040640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 571
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 560
AccI GTMKAC 1 cut(s) 757
AciI CCGC 2 cut(s) 211, 358
AclWI GGATC 7 cut(s) 60, 227, 240, 247, 260, 400, 413
AcsI RAATTY 2 cut(s) 383, 451
AcyI GRCGYC 1 cut(s) 566
AfaI GTAC 4 cut(s) 6, 188, 294, 606
AfeI AGCGCT 1 cut(s) 349
AfiI CCNNNNNNNGG 4 cut(s) 296, 560, 601, 698
AgeI ACCGGT 1 cut(s) 767
AgsI TTSAA 5 cut(s) 133, 389, 620, 631, 811
AjnI CCWGG 1 cut(s) 584
AluBI AGCT 2 cut(s) 287, 725
AluI AGCT 2 cut(s) 287, 725
Alw21I GWGCWC 3 cut(s) 289, 682, 798
Alw26I GTCTC 2 cut(s) 201, 651
Alw44I GTGCAC 1 cut(s) 794
AlwI GGATC 7 cut(s) 60, 227, 240, 247, 260, 400, 413
Aor51HI AGCGCT 1 cut(s) 349
AoxI GGCC 3 cut(s) 44, 298, 582
ApaLI GTGCAC 1 cut(s) 794
ApeKI GCWGC 2 cut(s) 430, 722
ApoI RAATTY 2 cut(s) 383, 451
AsiGI ACCGGT 1 cut(s) 767
Asp718I GGTACC 1 cut(s) 4
AspLEI GCGC 1 cut(s) 350
AspS9I GGNCC 2 cut(s) 266, 659
AsuHPI GGTGA 3 cut(s) 214, 757, 788
AsuII TTCGAA 2 cut(s) 445, 498
AvaII GGWCC 2 cut(s) 266, 659
BaeGI GKGCMC 1 cut(s) 798
BamHI GGATCC 3 cut(s) 232, 252, 405
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 289
BauI CACGAG 1 cut(s) 66
Bbv12I GWGCWC 3 cut(s) 289, 682, 798
BbvI GCAGC 2 cut(s) 442, 734
BccI CCATC 2 cut(s) 461, 661
BceAI ACGGC 2 cut(s) 135, 553
BciT130I CCWGG 1 cut(s) 586
BcoDI GTCTC 2 cut(s) 201, 651
BfaI CTAG 2 cut(s) 123, 704
BfmI CTRYAG 1 cut(s) 428
BfoI RGCGCY 1 cut(s) 351
BisI GCNGC 2 cut(s) 431, 723
BlsI GCNGC 2 cut(s) 432, 724
Bme1390I CCNGG 1 cut(s) 586
Bme18I GGWCC 2 cut(s) 266, 659
BmgT120I GGNCC 2 cut(s) 266, 659
BmiI GGNNCC 4 cut(s) 6, 234, 254, 407
BmrFI CCNGG 1 cut(s) 586
BmsI GCATC 1 cut(s) 812
Bpu14I TTCGAA 2 cut(s) 445, 498
BpuEI CTTGAG 1 cut(s) 352
Bsa29I ATCGAT 1 cut(s) 30
BsaAI YACGTR 1 cut(s) 296
BsaBI GATNNNNATC 1 cut(s) 404
BsaHI GRCGYC 1 cut(s) 566
BsaI GGTCTC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 269
BsaWI WCCGGW 2 cut(s) 289, 767
Bsc4I CCNNNNNNNGG 4 cut(s) 296, 560, 601, 698
Bse118I RCCGGY 3 cut(s) 46, 601, 767
Bse8I GATNNNNATC 1 cut(s) 404
BseBI CCWGG 1 cut(s) 586
BseCI ATCGAT 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 269
BseGI GGATG 1 cut(s) 469
BseJI GATNNNNATC 1 cut(s) 404
BseLI CCNNNNNNNGG 4 cut(s) 296, 560, 601, 698
BseMII CTCAG 1 cut(s) 209
BseSI GKGCMC 1 cut(s) 798
BseXI GCAGC 2 cut(s) 442, 734
BsgI GTGCAG 1 cut(s) 386
BshFI GGCC 3 cut(s) 46, 300, 584
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 767
BshVI ATCGAT 1 cut(s) 30
BsiHKAI GWGCWC 3 cut(s) 289, 682, 798
BsiSI CCGG 5 cut(s) 47, 160, 290, 602, 768
BslFI GGGAC 1 cut(s) 577
BslI CCNNNNNNNGG 4 cut(s) 296, 560, 601, 698
BsmAI GTCTC 2 cut(s) 201, 651
BsmFI GGGAC 1 cut(s) 577
BsnI GGCC 3 cut(s) 46, 300, 584
Bso31I GGTCTC 1 cut(s) 201
Bsp119I TTCGAA 2 cut(s) 445, 498
Bsp1286I GDGCHC 3 cut(s) 289, 682, 798
Bsp143I GATC 7 cut(s) 52, 140, 232, 252, 322, 405, 421
BspACI CCGC 2 cut(s) 211, 358
BspANI GGCC 3 cut(s) 46, 300, 584
BspCNI CTCAG 1 cut(s) 208
BspDI ATCGAT 1 cut(s) 30
BspLI GGNNCC 4 cut(s) 6, 234, 254, 407
BspMAI CTGCAG 1 cut(s) 432
BspPI GGATC 7 cut(s) 60, 227, 240, 247, 260, 400, 413
BspT104I TTCGAA 2 cut(s) 445, 498
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 201
BsrFI RCCGGY 3 cut(s) 46, 601, 767
BssAI RCCGGY 3 cut(s) 46, 601, 767
BssECI CCNNGG 1 cut(s) 269
BssMI GATC 7 cut(s) 52, 140, 232, 252, 322, 405, 421
BssNI GRCGYC 1 cut(s) 566
BssSI CACGAG 1 cut(s) 66
BssT1I CCWWGG 1 cut(s) 269
Bst2BI CACGAG 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 586
Bst4CI ACNGT 4 cut(s) 186, 245, 663, 684
BstACI GRCGYC 1 cut(s) 566
BstBAI YACGTR 1 cut(s) 296
BstBI TTCGAA 2 cut(s) 445, 498
BstC8I GCNNGC 3 cut(s) 165, 302, 720
BstDEI CTNAG 1 cut(s) 195
BstF5I GGATG 1 cut(s) 469
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 1 cut(s) 350
BstKTI GATC 7 cut(s) 55, 143, 235, 255, 325, 408, 424
BstMAI GTCTC 2 cut(s) 201, 651
BstMBI GATC 7 cut(s) 52, 140, 232, 252, 322, 405, 421
BstMWI GCNNNNNNNGC 1 cut(s) 364
BstNI CCWGG 1 cut(s) 586
BstNSI RCATGY 2 cut(s) 157, 167
BstSCI CCNGG 1 cut(s) 584
BstSFI CTRYAG 1 cut(s) 428
BstSLI GKGCMC 1 cut(s) 798
BstV1I GCAGC 2 cut(s) 442, 734
BstX2I RGATCY 4 cut(s) 52, 232, 252, 405
BstYI RGATCY 4 cut(s) 52, 232, 252, 405
Bsu15I ATCGAT 1 cut(s) 30
BsuRI GGCC 3 cut(s) 46, 300, 584
BsuTUI ATCGAT 1 cut(s) 30
BtsCI GGATG 1 cut(s) 469
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 3 cut(s) 165, 302, 720
CfoI GCGC 1 cut(s) 350
Cfr10I RCCGGY 3 cut(s) 46, 601, 767
Cfr13I GGNCC 2 cut(s) 266, 659
ClaI ATCGAT 1 cut(s) 30
CseI GACGC 1 cut(s) 574
Csp6I GTAC 4 cut(s) 5, 187, 293, 605
CspAI ACCGGT 1 cut(s) 767
CviQI GTAC 4 cut(s) 5, 187, 293, 605
DdeI CTNAG 1 cut(s) 195
DpnI GATC 7 cut(s) 54, 142, 234, 254, 324, 407, 423
DpnII GATC 7 cut(s) 52, 140, 232, 252, 322, 405, 421
DrdI GACNNNNNNGTC 1 cut(s) 571
DseDI GACNNNNNNGTC 1 cut(s) 571
Ecl136II GAGCTC 1 cut(s) 287
Eco130I CCWWGG 1 cut(s) 269
Eco24I GRGCYC 1 cut(s) 289
Eco31I GGTCTC 1 cut(s) 201
Eco47I GGWCC 2 cut(s) 266, 659
Eco47III AGCGCT 1 cut(s) 349
Eco53kI GAGCTC 1 cut(s) 287
EcoICRI GAGCTC 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 584
EcoT14I CCWWGG 1 cut(s) 269
EcoT22I ATGCAT 2 cut(s) 169, 805
EcoT38I GRGCYC 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 269
FaqI GGGAC 1 cut(s) 577
FblI GTMKAC 1 cut(s) 757
Fnu4HI GCNGC 2 cut(s) 431, 723
FokI GGATG 1 cut(s) 476
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 2 cut(s) 431, 723
FspBI CTAG 2 cut(s) 123, 704
GlaI GCGC 1 cut(s) 349
GluI GCNGC 2 cut(s) 431, 723
HaeII RGCGCY 1 cut(s) 351
HaeIII GGCC 3 cut(s) 46, 300, 584
HapII CCGG 5 cut(s) 47, 160, 290, 602, 768
HgaI GACGC 1 cut(s) 574
HhaI GCGC 1 cut(s) 350
Hin1I GRCGYC 1 cut(s) 566
Hin6I GCGC 1 cut(s) 348
HinP1I GCGC 1 cut(s) 348
HincII GTYRAC 2 cut(s) 117, 758
HindII GTYRAC 2 cut(s) 117, 758
HinfI GANTC 3 cut(s) 400, 688, 754
HpaII CCGG 5 cut(s) 47, 160, 290, 602, 768
HphI GGTGA 3 cut(s) 214, 757, 788
Hpy166II GTNNAC 5 cut(s) 117, 189, 692, 758, 796
Hpy188I TCNGA 5 cut(s) 93, 198, 252, 405, 410
Hpy188III TCNNGA 2 cut(s) 521, 617
Hpy8I GTNNAC 5 cut(s) 117, 189, 692, 758, 796
Hpy99I CGWCG 2 cut(s) 612, 667
HpyAV CCTTC 6 cut(s) 266, 330, 364, 386, 390, 441
HpyCH4III ACNGT 4 cut(s) 186, 245, 663, 684
HpyCH4IV ACGT 2 cut(s) 95, 295
HpyCH4V TGCA 5 cut(s) 167, 367, 430, 796, 803
HpyF10VI GCNNNNNNNGC 1 cut(s) 364
HpyF3I CTNAG 1 cut(s) 195
HpySE526I ACGT 2 cut(s) 95, 295
Hsp92I GRCGYC 1 cut(s) 566
HspAI GCGC 1 cut(s) 348
KpnI GGTACC 1 cut(s) 8
Kzo9I GATC 7 cut(s) 52, 140, 232, 252, 322, 405, 421
LmnI GCTCC 2 cut(s) 292, 677
Lsp1109I GCAGC 2 cut(s) 442, 734
LweI GCATC 1 cut(s) 812
MaeI CTAG 2 cut(s) 123, 704
MaeII ACGT 2 cut(s) 95, 295
MaeIII GTNAC 1 cut(s) 763
MalI GATC 7 cut(s) 54, 142, 234, 254, 324, 407, 423
MboI GATC 7 cut(s) 52, 140, 232, 252, 322, 405, 421
MboII GAAGA 6 cut(s) 47, 401, 416, 487, 525, 791
MflI RGATCY 4 cut(s) 52, 232, 252, 405
MhlI GDGCHC 3 cut(s) 289, 682, 798
MluCI AATT 3 cut(s) 383, 451, 747
MlyI GAGTC 2 cut(s) 697, 763
MmeI TCCRAC 2 cut(s) 260, 637
MnlI CCTC 4 cut(s) 67, 165, 515, 574
Mph1103I ATGCAT 2 cut(s) 169, 805
MseI TTAA 1 cut(s) 227
MslI CAYNNNNRTG 1 cut(s) 152
MspI CCGG 5 cut(s) 47, 160, 290, 602, 768
MspR9I CCNGG 1 cut(s) 586
MvaI CCWGG 1 cut(s) 586
MwoI GCNNNNNNNGC 1 cut(s) 364
NdeII GATC 7 cut(s) 52, 140, 232, 252, 322, 405, 421
NlaIV GGNNCC 4 cut(s) 6, 234, 254, 407
NmuCI GTSAC 1 cut(s) 763
NsiI ATGCAT 2 cut(s) 169, 805
NspI RCATGY 2 cut(s) 157, 167
NspV TTCGAA 2 cut(s) 445, 498
PaeI GCATGC 1 cut(s) 167
PcsI WCGNNNNNNNCGW 2 cut(s) 64, 256
PfeI GAWTC 1 cut(s) 400
PflMI CCANNNNNTGG 1 cut(s) 560
PinAI ACCGGT 1 cut(s) 767
PkrI GCNGC 2 cut(s) 432, 724
PleI GAGTC 2 cut(s) 696, 762
PpsI GAGTC 2 cut(s) 696, 762
Ppu21I YACGTR 1 cut(s) 296
Psp124BI GAGCTC 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 584
PspGI CCWGG 1 cut(s) 584
PspN4I GGNNCC 4 cut(s) 6, 234, 254, 407
PspPI GGNCC 2 cut(s) 266, 659
PstI CTGCAG 1 cut(s) 432
PsuI RGATCY 4 cut(s) 52, 232, 252, 405
RsaI GTAC 4 cut(s) 6, 188, 294, 606
RsaNI GTAC 4 cut(s) 5, 187, 293, 605
RseI CAYNNNNRTG 1 cut(s) 152
SacI GAGCTC 1 cut(s) 289
SalI GTCGAC 1 cut(s) 756
SaqAI TTAA 1 cut(s) 227
SatI GCNGC 2 cut(s) 431, 723
Sau3AI GATC 7 cut(s) 52, 140, 232, 252, 322, 405, 421
Sau96I GGNCC 2 cut(s) 266, 659
SchI GAGTC 2 cut(s) 697, 763
ScrFI CCNGG 1 cut(s) 586
SduI GDGCHC 3 cut(s) 289, 682, 798
SetI ASST 8 cut(s) 10, 98, 227, 289, 298, 507, 657, 727
SfaNI GCATC 1 cut(s) 812
SfcI CTRYAG 1 cut(s) 428
SfuI TTCGAA 2 cut(s) 445, 498
SinI GGWCC 2 cut(s) 266, 659
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 1 cut(s) 331
SmoI CTYRAG 1 cut(s) 331
SphI GCATGC 1 cut(s) 167
Sse9I AATT 3 cut(s) 383, 451, 747
SsiI CCGC 2 cut(s) 211, 358
SspMI CTAG 2 cut(s) 123, 704
SstI GAGCTC 1 cut(s) 289
StyD4I CCNGG 1 cut(s) 584
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 4 cut(s) 186, 245, 663, 684
TaiI ACGT 2 cut(s) 98, 298
TaqI TCGA 7 cut(s) 30, 259, 445, 498, 665, 686, 757
TasI AATT 3 cut(s) 383, 451, 747
TatI WGTACW 1 cut(s) 186
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 1 cut(s) 227
Tru9I TTAA 1 cut(s) 227
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 1 cut(s) 763
TseI GCWGC 2 cut(s) 430, 722
Tsp45I GTSAC 1 cut(s) 763
TspDTI ATGAA 3 cut(s) 136, 305, 445
TspGWI ACGGA 1 cut(s) 245
TspRI CASTG 1 cut(s) 196
Van91I CCANNNNNTGG 1 cut(s) 560
VneI GTGCAC 1 cut(s) 794
VpaK11BI GGWCC 2 cut(s) 266, 659
XapI RAATTY 2 cut(s) 383, 451
XceI RCATGY 2 cut(s) 157, 167
XmiI GTMKAC 1 cut(s) 757
XspI CTAG 2 cut(s) 123, 704
Zsp2I ATGCAT 2 cut(s) 169, 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.