RorugPtG0003200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000008
OG0000336
Physical Location & Seq
Forward (+)
59065 .. 61030
1966 bp
Loading structure...
UTR
Exon/CDS
Intron
RorugPtG0003200.1

Sequence Viewer

Length: 1464 bp
ATGGAAAAATGGTGGTTCGGTTCGCTCTTGTTTAGCGGGGAGTTAGAATACAGGTGTAGGCTAAGTAAATCAATGGCCAGTTTTGGTCCTTTTGAAAATCCCAGTGTAAGTGAAGATCCAATTATAGATGATATGGATAAAGACGTGTCTAATTGTAGTGACAAGTCTAATTACAGTAATGTTGATCATTTAGTCGGCGGCGGATCCATTCGGAATTTAATATCGGATGAGACTTTTTTCGTTATGGATAGTAATAGGGACGGTTATTCCATATATTTTGATATTGAAAATCAAAATTGTGAGATTGACACCGATCATTCTTTTCTAAGTGAACTAAAAAGTTCGTTTTATAGTTATCAGACTTCGAGTTATATGAATAATGCAACTAAAAGTGACGATAATCGCCACAACCGTTACATGTATGATACTAATTCTAATTATAGTTGGAATAATCACATTACTAGTTGCATTGACAGTTATCTTCGTTCTCACATTTGTATTGATAGTTATATTTTAAGCAATAGTGACAATTACAGTGATAGTTACATTTATAGTTACATTTGTGGCGAAAGCGGAAATAGTAGTAAAAGCGGGAGTTCCAGTATCAAAACTAGCAAAGATGGTATAAGATCTAATAATTTAAATGTAACTCAAAAATACAGGCATTTGTGGATTCAATGCGAAAATTGTTATGGATTAAATTATAAGAAATTTTTAAAATCCAAAATGAATATTTGTGAACAGTGTGGATGTCATTTGAAAATGAGTAGTTTCGATAGAATCGAAAGTTCGATTGATCCAGGTACTTGGGATCCTATGAATGAAGACATGGTCTCTCTGGATCCCATTCAATTTCATTCGGAGGAGGAACCTTATAAGGATCGTATTGATTCTTATCAAAAAAATACAGGATTAACTGAGGCTGTTCAAACAGGCACAGGTCAATTAAATGGCATTCCCGTAGCAATTGGGGTTATGGATTTTCAGTTTATGGGGGGTAGTATGGGATCCGTAGTAGGTGAAAAAATCACACGTTTGGCTGAGTATGCTACCAATAAACTTTTACCTCTTATTCTAGTGTGTGCTTCCGGAGGAGCACGTATGCAAGAAGGAAGTTTGAGCTTGATGCAAATGGCTAAAATATCTTCCGCTTTATACGATTATCAATCAAATAAAAAGTTATTTTATGTCGCAGTCCTTACATCCCCTACCACAGGTGGGGTGACGGCTAGTTTTGGTATGCTGGGAGATATCATTATTGCTGAACCCAACGCTTACATTGCATTTGCGGGTAAAAGAGTAATTGAACAAACATTGAATAAGACAGTACCCGAGGATTCACAAGTGGCTGAATATTTATTCCATAAGGGCTTATTCGATCCAATCGTACCACGTAATCCTTTAAAGGGCGTTCTGAGTGAATTATTTCGGCTACATGCCTTCTTTCCTTTGAATCAAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

487

Amino Acids

54.78

Weight (kDa)

5.06

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carboxyl_trans PF01039 280 - 448 2.4e-21 Carboxyl transferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000336)

Species Orthologous Gene IDs
malus_domestica MD05G1024100.v1.1
rosa_chinensis RchiOBHm_Chr2g0152291 RchiOBHm_Chr4g0387411
rosa_laevigata RLG00000009698 RLG00000009699 RLG00000009700 RLG00000023615 RLG00000024265 RLG00000034445 RLG00000036567
rosa_multiflora Rmu_co8268067.1_g000001 Rmu_co8313555.1_g000001 Rmu_co8324697.1_g000001 Rmu_sc0000318.1_g000023 Rmu_sc0000342.1_g000016 Rmu_sc0000447.1_g000010 Rmu_sc0000528.1_g000009 Rmu_sc0000539.1_g000060 Rmu_sc0000929.1_g000023 Rmu_sc0001493.1_g000081 Rmu_sc0001674.1_g000003 Rmu_sc0001791.1_g000023 Rmu_sc0001822.1_g000005 Rmu_sc0001822.1_g000033 Rmu_sc0002061.1_g000010 Rmu_sc0002286.1_g000012 Rmu_sc0002979.1_g000009 Rmu_sc0003158.1_g000016 Rmu_sc0003221.1_g000001 Rmu_sc0003641.1_g000029 Rmu_sc0003742.1_g000012 Rmu_sc0003814.1_g000004 Rmu_sc0003823.1_g000005 Rmu_sc0004002.1_g000005 Rmu_sc0004564.1_g000005 Rmu_sc0005415.1_g000003 Rmu_sc0006050.1_g000025 Rmu_sc0006555.1_g000002 Rmu_sc0009954.1_g000020 Rmu_sc0010421.1_g000007 Rmu_sc0010607.1_g000002 Rmu_sc0011044.1_g000005 Rmu_sc0011497.1_g000015 Rmu_sc0014532.1_g000004 Rmu_sc0014745.1_g000005 Rmu_sc0014821.1_g000001 Rmu_sc0015038.1_g000002 Rmu_sc0015454.1_g000004 Rmu_sc0019663.1_g000002 Rmu_sc0027475.1_g000003 Rmu_sc0028826.1_g000002 Rmu_ssc0000117.1_g000019 Rmu_ssc0000175.1_g000034
rosa_roxburghii Rroxscaffold_3G00237080 Rroxscaffold_4G00288290 Rroxscaffold_6G00397510
rosa_rugosa Rorug01G0035000 Rorug01G0190900 Rorug01G0208500 Rorug01G0208600 Rorug01G0208600 Rorug01G0344900 Rorug01G0479500 Rorug01G0479600 Rorug02G0230400 Rorug02G0320900 Rorug02G0433300 Rorug03G0147400 Rorug03G0169700.1 Rorug03G0169800 Rorug03G0198300 Rorug04G0022900 Rorug04G0064600 Rorug04G0322100 Rorug04G0388900 Rorug05G0153200 Rorug05G0231600 Rorug05G0231700 Rorug05G0231800 Rorug05G0276200 Rorug05G0292400 Rorug05G0314700 Rorug06G0092000 Rorug06G0260900 Rorug06G0273600 RorugPtG0003200
rosa_samantha Rh1AG264100 Rh1AG292500 Rh1AG423800 Rh2AG098000 Rh2AG232700 Rh2AG361100 Rh2AG372900 Rh2BG099200 Rh2BG246000 Rh2BG260600 Rh2BG378400 Rh3BG142300 Rh3BG252900 Rh3BG267700 Rh3DG302400 Rh5BG059800 Rh5BG320000 Rh5BG530500 Rh6AG089300 Rh6AG089400 Rh6AG127400 Rh6AG376700 Rh7CG488700 Rh7DG265900
rosa_wichuraiana Rw2G040640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 705, 876
AccIII TCCGGA 1 cut(s) 1088
AciI CCGC 7 cut(s) 36, 198, 201, 573, 591, 1149, 1289
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 2 cut(s) 214, 710
AfaI GTAC 3 cut(s) 805, 1329, 1389
AfiI CCNNNNNNNGG 3 cut(s) 1214, 1218, 1406
AflIII ACRYGT 3 cut(s) 144, 417, 1031
AgsI TTSAA 9 cut(s) 95, 287, 677, 760, 851, 929, 1307, 1318, 1453
AhlI ACTAGT 1 cut(s) 461
AjiI CACGTC 1 cut(s) 145
AjnI CCWGG 1 cut(s) 799
AluBI AGCT 1 cut(s) 1122
AluI AGCT 1 cut(s) 1122
Alw21I GWGCWC 1 cut(s) 1099
Alw26I GTCTC 2 cut(s) 224, 838
Ama87I CYCGRG 1 cut(s) 1331
Aor13HI TCCGGA 1 cut(s) 1088
AoxI GGCC 1 cut(s) 75
ApoI RAATTY 2 cut(s) 214, 710
Asp700I GAANNNNTTC 1 cut(s) 1425
AspS9I GGNCC 1 cut(s) 86
AsuHPI GGTGA 2 cut(s) 1031, 1234
AvaI CYCGRG 1 cut(s) 1331
AvaII GGWCC 1 cut(s) 86
BalI TGGCCA 1 cut(s) 77
BamHI GGATCC 4 cut(s) 203, 811, 841, 1007
BbsI GAAGAC 1 cut(s) 831
Bbv12I GWGCWC 1 cut(s) 1099
BccI CCATC 1 cut(s) 614
BceAI ACGGC 1 cut(s) 1242
BciT130I CCWGG 1 cut(s) 801
BclI TGATCA 1 cut(s) 184
BcoDI GTCTC 2 cut(s) 224, 838
BcuI ACTAGT 1 cut(s) 461
BfaI CTAG 4 cut(s) 462, 612, 1076, 1230
BglII AGATCT 1 cut(s) 629
BisI GCNGC 1 cut(s) 199
BlsI GCNGC 1 cut(s) 200
Bme1390I CCNGG 1 cut(s) 801
Bme18I GGWCC 1 cut(s) 86
BmeT110I CYCGRG 1 cut(s) 1331
BmgBI CACGTC 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 5 cut(s) 205, 813, 843, 870, 1009
BmrFI CCNGG 1 cut(s) 801
BmrI ACTGGG 1 cut(s) 96
BmsI GCATC 1 cut(s) 1116
BmuI ACTGGG 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 831
BsaAI YACGTR 2 cut(s) 1100, 1394
BsaBI GATNNNNATC 1 cut(s) 894
BsaI GGTCTC 1 cut(s) 838
BsaJI CCNNGG 1 cut(s) 1332
BsaWI WCCGGW 1 cut(s) 1088
BsaXI ACNNNNNCTCC 2 cut(s) 586, 616
Bsc4I CCNNNNNNNGG 3 cut(s) 1214, 1218, 1406
Bse1I ACTGG 3 cut(s) 78, 102, 600
Bse3DI GCAATG 1 cut(s) 1278
Bse8I GATNNNNATC 1 cut(s) 894
BseAI TCCGGA 1 cut(s) 1088
BseBI CCWGG 1 cut(s) 801
BseDI CCNNGG 1 cut(s) 1332
BseGI GGATG 3 cut(s) 232, 755, 1202
BseJI GATNNNNATC 1 cut(s) 894
BseLI CCNNNNNNNGG 3 cut(s) 1214, 1218, 1406
BseMI GCAATG 1 cut(s) 1278
BseMII CTCAG 3 cut(s) 909, 1032, 1406
BseNI ACTGG 3 cut(s) 78, 102, 600
BseRI GAGGAG 2 cut(s) 878, 1107
BseYI CCCAGC 1 cut(s) 1243
BshFI GGCC 1 cut(s) 77
BsiHKAI GWGCWC 1 cut(s) 1099
BsiHKCI CYCGRG 1 cut(s) 1331
BsiSI CCGG 1 cut(s) 1089
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 3 cut(s) 1214, 1218, 1406
BsmAI GTCTC 2 cut(s) 224, 838
BsmFI GGGAC 1 cut(s) 272
BsmI GAATGC 1 cut(s) 954
BsnI GGCC 1 cut(s) 77
Bso31I GGTCTC 1 cut(s) 838
BsoBI CYCGRG 1 cut(s) 1331
Bsp1286I GDGCHC 1 cut(s) 1099
Bsp13I TCCGGA 1 cut(s) 1088
BspACI CCGC 7 cut(s) 36, 198, 201, 573, 591, 1149, 1289
BspANI GGCC 1 cut(s) 77
BspCNI CTCAG 3 cut(s) 910, 1033, 1407
BspEI TCCGGA 1 cut(s) 1088
BspLI GGNNCC 5 cut(s) 205, 813, 843, 870, 1009
BspTNI GGTCTC 1 cut(s) 838
BsrDI GCAATG 1 cut(s) 1278
BsrI ACTGG 3 cut(s) 78, 102, 600
BssECI CCNNGG 1 cut(s) 1332
Bst2UI CCWGG 1 cut(s) 801
Bst4CI ACNGT 7 cut(s) 176, 263, 413, 476, 536, 744, 1327
BstBAI YACGTR 2 cut(s) 1100, 1394
BstDEI CTNAG 6 cut(s) 62, 326, 918, 1041, 1415, 1461
BstENI CCTNNNNNAGG 1 cut(s) 1212
BstF5I GGATG 3 cut(s) 232, 755, 1202
BstMAI GTCTC 2 cut(s) 224, 838
BstMWI GCNNNNNNNGC 2 cut(s) 1046, 1280
BstNI CCWGG 1 cut(s) 801
BstNSI RCATGY 2 cut(s) 421, 1439
BstSCI CCNGG 1 cut(s) 799
BstV2I GAAGAC 1 cut(s) 831
BstX2I RGATCY 6 cut(s) 115, 203, 629, 811, 841, 1007
BstXI CCANNNNNNTGG 1 cut(s) 807
BstYI RGATCY 6 cut(s) 115, 203, 629, 811, 841, 1007
BsuRI GGCC 1 cut(s) 77
BtrI CACGTC 1 cut(s) 145
BtsCI GGATG 3 cut(s) 232, 755, 1202
BtsIMutI CAGTG 3 cut(s) 109, 541, 749
Cfr13I GGNCC 1 cut(s) 86
Csp6I GTAC 3 cut(s) 804, 1328, 1388
CviAII CATG 3 cut(s) 418, 829, 1436
CviQI GTAC 3 cut(s) 804, 1328, 1388
DdeI CTNAG 6 cut(s) 62, 326, 918, 1041, 1415, 1461
DraI TTTAAA 3 cut(s) 642, 717, 1404
EaeI YGGCCR 1 cut(s) 75
EciI GGCGGA 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 838
Eco32I GATATC 1 cut(s) 1252
Eco47I GGWCC 1 cut(s) 86
Eco88I CYCGRG 1 cut(s) 1331
EcoNI CCTNNNNNAGG 1 cut(s) 1212
EcoRII CCWGG 1 cut(s) 799
EcoRV GATATC 1 cut(s) 1252
FaeI CATG 3 cut(s) 421, 832, 1439
FaqI GGGAC 1 cut(s) 272
FatI CATG 3 cut(s) 417, 828, 1435
FauI CCCGC 3 cut(s) 29, 584, 1282
FbaI TGATCA 1 cut(s) 184
Fnu4HI GCNGC 1 cut(s) 199
FokI GGATG 3 cut(s) 239, 762, 1189
Fsp4HI GCNGC 1 cut(s) 199
FspBI CTAG 4 cut(s) 462, 612, 1076, 1230
GluI GCNGC 1 cut(s) 199
GsaI CCCAGC 1 cut(s) 1247
HaeIII GGCC 1 cut(s) 77
HapII CCGG 1 cut(s) 1089
Hin1II CATG 3 cut(s) 421, 832, 1439
HinfI GANTC 5 cut(s) 673, 780, 890, 1337, 1453
HpaII CCGG 1 cut(s) 1089
HphI GGTGA 2 cut(s) 1031, 1234
Hpy166II GTNNAC 2 cut(s) 332, 740
Hpy188I TCNGA 5 cut(s) 213, 226, 360, 862, 1416
Hpy188III TCNNGA 2 cut(s) 839, 1089
Hpy8I GTNNAC 2 cut(s) 332, 740
HpyAV CCTTC 2 cut(s) 1103, 1450
HpyCH4III ACNGT 7 cut(s) 176, 263, 413, 476, 536, 744, 1327
HpyCH4IV ACGT 4 cut(s) 144, 1033, 1099, 1393
HpyCH4V TGCA 5 cut(s) 383, 468, 1105, 1129, 1283
HpyF10VI GCNNNNNNNGC 2 cut(s) 1046, 1280
HpyF3I CTNAG 6 cut(s) 62, 326, 918, 1041, 1415, 1461
HpySE526I ACGT 4 cut(s) 144, 1033, 1099, 1393
Hsp92II CATG 3 cut(s) 421, 832, 1439
Kpn2I TCCGGA 1 cut(s) 1088
Ksp22I TGATCA 1 cut(s) 184
LmnI GCTCC 1 cut(s) 1094
LweI GCATC 1 cut(s) 1116
MaeI CTAG 4 cut(s) 462, 612, 1076, 1230
MaeII ACGT 4 cut(s) 144, 1033, 1099, 1393
MaeIII GTNAC 8 cut(s) 158, 392, 413, 524, 542, 554, 646, 1222
MboII GAAGA 4 cut(s) 125, 473, 836, 1137
MfeI CAATTG 1 cut(s) 966
MflI RGATCY 6 cut(s) 115, 203, 629, 811, 841, 1007
MhlI GDGCHC 1 cut(s) 1099
MlsI TGGCCA 1 cut(s) 77
MluNI TGGCCA 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 425
MnlI CCTC 6 cut(s) 856, 859, 913, 1077, 1085, 1327
Mox20I TGGCCA 1 cut(s) 77
MroI TCCGGA 1 cut(s) 1088
MroXI GAANNNNTTC 1 cut(s) 1425
MscI TGGCCA 1 cut(s) 77
MseI TTAA 8 cut(s) 218, 515, 641, 698, 716, 914, 947, 1403
Msp20I TGGCCA 1 cut(s) 77
MspI CCGG 1 cut(s) 1089
MspR9I CCNGG 1 cut(s) 801
MunI CAATTG 1 cut(s) 966
Mva1269I GAATGC 1 cut(s) 954
MvaI CCWGG 1 cut(s) 801
MwoI GCNNNNNNNGC 2 cut(s) 1046, 1280
NlaIII CATG 3 cut(s) 421, 832, 1439
NlaIV GGNNCC 5 cut(s) 205, 813, 843, 870, 1009
NmuCI GTSAC 4 cut(s) 158, 392, 524, 1222
NspI RCATGY 2 cut(s) 421, 1439
PciI ACATGT 1 cut(s) 417
PcsI WCGNNNNNNNCGW 3 cut(s) 409, 780, 1383
PctI GAATGC 1 cut(s) 954
PdmI GAANNNNTTC 1 cut(s) 1425
PfeI GAWTC 5 cut(s) 673, 780, 890, 1337, 1453
PflFI GACNNNGTC 1 cut(s) 830
PkrI GCNGC 1 cut(s) 200
Ppu21I YACGTR 2 cut(s) 1100, 1394
PscI ACATGT 1 cut(s) 417
PsiI TTATAA 2 cut(s) 705, 876
Psp6I CCWGG 1 cut(s) 799
PspFI CCCAGC 1 cut(s) 1243
PspGI CCWGG 1 cut(s) 799
PspN4I GGNNCC 5 cut(s) 205, 813, 843, 870, 1009
PspPI GGNCC 1 cut(s) 86
PsuI RGATCY 6 cut(s) 115, 203, 629, 811, 841, 1007
PsyI GACNNNGTC 1 cut(s) 830
RsaI GTAC 3 cut(s) 805, 1329, 1389
RsaNI GTAC 3 cut(s) 804, 1328, 1388
SaqAI TTAA 8 cut(s) 218, 515, 641, 698, 716, 914, 947, 1403
SatI GCNGC 1 cut(s) 199
Sau96I GGNCC 1 cut(s) 86
ScrFI CCNGG 1 cut(s) 801
SduI GDGCHC 1 cut(s) 1099
SfaNI GCATC 1 cut(s) 1116
SinI GGWCC 1 cut(s) 86
SmiI ATTTAAAT 1 cut(s) 642
SpeI ACTAGT 1 cut(s) 461
SsiI CCGC 7 cut(s) 36, 198, 201, 573, 591, 1149, 1289
SspI AATATT 2 cut(s) 733, 1355
SspMI CTAG 4 cut(s) 462, 612, 1076, 1230
StyD4I CCNGG 1 cut(s) 799
SwaI ATTTAAAT 1 cut(s) 642
TaaI ACNGT 7 cut(s) 176, 263, 413, 476, 536, 744, 1327
TaiI ACGT 4 cut(s) 147, 1036, 1102, 1396
TaqI TCGA 5 cut(s) 365, 774, 783, 791, 1377
TauI GCSGC 1 cut(s) 201
TfiI GAWTC 5 cut(s) 673, 780, 890, 1337, 1453
Tru1I TTAA 8 cut(s) 218, 515, 641, 698, 716, 914, 947, 1403
Tru9I TTAA 8 cut(s) 218, 515, 641, 698, 716, 914, 947, 1403
TscAI CASTG 3 cut(s) 109, 541, 749
TseFI GTSAC 4 cut(s) 158, 392, 524, 1222
Tsp45I GTSAC 4 cut(s) 158, 392, 524, 1222
TspDTI ATGAA 5 cut(s) 389, 743, 833, 837, 845
TspGWI ACGGA 1 cut(s) 1000
TspRI CASTG 3 cut(s) 109, 541, 749
Tth111I GACNNNGTC 1 cut(s) 830
VpaK11BI GGWCC 1 cut(s) 86
XagI CCTNNNNNAGG 1 cut(s) 1212
XapI RAATTY 2 cut(s) 214, 710
XceI RCATGY 2 cut(s) 421, 1439
XmnI GAANNNNTTC 1 cut(s) 1425
XspI CTAG 4 cut(s) 462, 612, 1076, 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.