RchiOBHm_Chr4g0447281

Protein of unknown function (DUF789)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0010858
Physical Location & Seq
Forward (+)
67102106 .. 67106648
4543 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 498 bp
ATGTTTATTGATCATATATGCAACGAAATGCTTGATCAGATCAATGAATTAGCTCAAATTTATCCTGGTTTGATGGAGTTTCAAAGCTTGGAGCTCTCTCCTTGTAGCTGGATGGCTGTTGCTTGGTATCCTATCTATCAAATTCCAGTTACAAGGAATGTGAAGGAATTATCTGCATATTTCATCACTTATCACACATTATCATCTTTTCAAGGCCTCCATAGTACCATATCAGAGAATAATGATAAGTTTCAATGCATTGATACATTGGGAAAGGATCATGGAAGCAAGACGCTAGAAAGAGATGAAAGCAAAGGTGAAATGGCTCTTCCTCCTTTTGCAGCGGCCACTTATAAGATGCATGGCGAACTTTGGACAACTCCTGAGACTTCGGATAAGGAAAAGATTGGCTTTTATCTGAACGCTGCAAATTCCTGGTTGAAGCATCGCAAATTCCGGCATCATGACTTTATTTCTTCATATCTCGTAGGTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF789

Protein Analysis

165

Amino Acids

19.06

Weight (kDa)

5.7

Isoelectric Point (pI)

34.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF789 PF05623 9 - 157 5.5e-45 Protein of unknown function (DUF789)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 354
AciI CCGC 1 cut(s) 344
AclWI GGATC 1 cut(s) 285
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 4 cut(s) 57, 141, 430, 452
AfaI GTAC 1 cut(s) 226
AgsI TTSAA 4 cut(s) 83, 212, 254, 442
AjnI CCWGG 2 cut(s) 64, 434
AluBI AGCT 4 cut(s) 53, 87, 94, 108
AluI AGCT 4 cut(s) 53, 87, 94, 108
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 380
AlwI GGATC 1 cut(s) 285
AoxI GGCC 2 cut(s) 214, 345
ApeKI GCWGC 2 cut(s) 341, 425
ApoI RAATTY 4 cut(s) 57, 141, 430, 452
AsuHPI GGTGA 1 cut(s) 329
BanII GRGCYC 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 2 cut(s) 353, 412
BccI CCATC 2 cut(s) 67, 106
BciT130I CCWGG 2 cut(s) 66, 436
BciVI GTATCC 1 cut(s) 138
BclI TGATCA 2 cut(s) 10, 34
BcoDI GTCTC 1 cut(s) 380
BfaI CTAG 1 cut(s) 296
BfuI GTATCC 1 cut(s) 138
BisI GCNGC 3 cut(s) 342, 345, 426
BlsI GCNGC 3 cut(s) 343, 346, 427
Bme1390I CCNGG 2 cut(s) 66, 436
BmrFI CCNGG 2 cut(s) 66, 436
BmsI GCATC 3 cut(s) 348, 454, 469
Bse1I ACTGG 1 cut(s) 146
BseBI CCWGG 2 cut(s) 66, 436
BseGI GGATG 1 cut(s) 117
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 1 cut(s) 146
BseXI GCAGC 2 cut(s) 353, 412
BshFI GGCC 2 cut(s) 216, 347
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 1 cut(s) 457
BsmAI GTCTC 1 cut(s) 380
BsnI GGCC 2 cut(s) 216, 347
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 4 cut(s) 10, 34, 39, 277
BspACI CCGC 1 cut(s) 344
BspANI GGCC 2 cut(s) 216, 347
BspCNI CTCAG 1 cut(s) 376
BspHI TCATGA 1 cut(s) 463
BspPI GGATC 1 cut(s) 285
BspQI GCTCTTC 1 cut(s) 333
BsrI ACTGG 1 cut(s) 146
BssMI GATC 4 cut(s) 10, 34, 39, 277
Bst2UI CCWGG 2 cut(s) 66, 436
Bst6I CTCTTC 1 cut(s) 333
BstDEI CTNAG 1 cut(s) 384
BstF5I GGATG 1 cut(s) 117
BstKTI GATC 4 cut(s) 13, 37, 42, 280
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 4 cut(s) 10, 34, 39, 277
BstNI CCWGG 2 cut(s) 66, 436
BstSCI CCNGG 2 cut(s) 64, 434
BstV1I GCAGC 2 cut(s) 353, 412
BsuI GTATCC 1 cut(s) 138
BsuRI GGCC 2 cut(s) 216, 347
BtgZI GCGATG 1 cut(s) 431
BtsCI GGATG 1 cut(s) 117
CciI TCATGA 1 cut(s) 463
CseI GACGC 1 cut(s) 301
Csp6I GTAC 1 cut(s) 225
CviAII CATG 3 cut(s) 281, 362, 464
CviJI RGCY 9 cut(s) 53, 87, 94, 108, 116, 216, 326, 347, 411
CviKI_1 RGCY 9 cut(s) 53, 87, 94, 108, 116, 216, 326, 347, 411
CviQI GTAC 1 cut(s) 225
DdeI CTNAG 1 cut(s) 384
DpnI GATC 4 cut(s) 12, 36, 41, 279
DpnII GATC 4 cut(s) 10, 34, 39, 277
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
Ecl136II GAGCTC 1 cut(s) 94
Eco147I AGGCCT 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 96
Eco53kI GAGCTC 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 94
EcoRII CCWGG 2 cut(s) 64, 434
EcoT22I ATGCAT 2 cut(s) 260, 363
EcoT38I GRGCYC 1 cut(s) 96
FaeI CATG 3 cut(s) 284, 365, 467
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FatI CATG 3 cut(s) 280, 361, 463
FbaI TGATCA 2 cut(s) 10, 34
Fnu4HI GCNGC 3 cut(s) 342, 345, 426
FokI GGATG 1 cut(s) 124
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 3 cut(s) 342, 345, 426
FspBI CTAG 1 cut(s) 296
GluI GCNGC 3 cut(s) 342, 345, 426
HaeIII GGCC 2 cut(s) 216, 347
HapII CCGG 1 cut(s) 457
HgaI GACGC 1 cut(s) 301
Hin1II CATG 3 cut(s) 284, 365, 467
HindIII AAGCTT 1 cut(s) 85
HpaII CCGG 1 cut(s) 457
HphI GGTGA 1 cut(s) 329
Hpy188I TCNGA 4 cut(s) 39, 235, 394, 420
Hpy188III TCNNGA 2 cut(s) 383, 464
HpyAV CCTTC 1 cut(s) 157
HpyCH4V TGCA 6 cut(s) 21, 176, 258, 341, 361, 428
HpyF3I CTNAG 1 cut(s) 384
Hsp92II CATG 3 cut(s) 284, 365, 467
Ksp22I TGATCA 2 cut(s) 10, 34
Kzo9I GATC 4 cut(s) 10, 34, 39, 277
LguI GCTCTTC 1 cut(s) 333
LmnI GCTCC 1 cut(s) 91
LpnPI CCDG 8 cut(s) 51, 78, 94, 159, 396, 421, 448, 470
Lsp1109I GCAGC 2 cut(s) 353, 412
LweI GCATC 3 cut(s) 348, 454, 469
MaeI CTAG 1 cut(s) 296
MaeIII GTNAC 2 cut(s) 148, 491
MalI GATC 4 cut(s) 12, 36, 41, 279
MboI GATC 4 cut(s) 10, 34, 39, 277
MboII GAAGA 2 cut(s) 320, 468
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 6 cut(s) 47, 57, 141, 167, 430, 452
MnlI CCTC 2 cut(s) 227, 342
Mph1103I ATGCAT 2 cut(s) 260, 363
MspA1I CMGCKG 1 cut(s) 344
MspI CCGG 1 cut(s) 457
MspR9I CCNGG 2 cut(s) 66, 436
MvaI CCWGG 2 cut(s) 66, 436
NdeII GATC 4 cut(s) 10, 34, 39, 277
NlaIII CATG 3 cut(s) 284, 365, 467
NsiI ATGCAT 2 cut(s) 260, 363
PagI TCATGA 1 cut(s) 463
PceI AGGCCT 1 cut(s) 216
PciSI GCTCTTC 1 cut(s) 333
PkrI GCNGC 3 cut(s) 343, 346, 427
PsiI TTATAA 1 cut(s) 354
Psp124BI GAGCTC 1 cut(s) 96
Psp6I CCWGG 2 cut(s) 64, 434
PspGI CCWGG 2 cut(s) 64, 434
RsaI GTAC 1 cut(s) 226
RsaNI GTAC 1 cut(s) 225
SacI GAGCTC 1 cut(s) 96
SapI GCTCTTC 1 cut(s) 333
SatI GCNGC 3 cut(s) 342, 345, 426
Sau3AI GATC 4 cut(s) 10, 34, 39, 277
ScrFI CCNGG 2 cut(s) 66, 436
SduI GDGCHC 1 cut(s) 96
SetI ASST 6 cut(s) 55, 89, 96, 110, 319, 493
SfaNI GCATC 3 cut(s) 348, 454, 469
Sse9I AATT 6 cut(s) 47, 57, 141, 167, 430, 452
SseBI AGGCCT 1 cut(s) 216
SsiI CCGC 1 cut(s) 344
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 1 cut(s) 96
StuI AGGCCT 1 cut(s) 216
StyD4I CCNGG 2 cut(s) 64, 434
TasI AATT 6 cut(s) 47, 57, 141, 167, 430, 452
TauI GCSGC 1 cut(s) 347
TseI GCWGC 2 cut(s) 341, 425
TspDTI ATGAA 4 cut(s) 60, 172, 321, 468
XapI RAATTY 4 cut(s) 57, 141, 430, 452
XspI CTAG 1 cut(s) 296
Zsp2I ATGCAT 2 cut(s) 260, 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.