Rh4DG448300

Protein of unknown function (DUF789)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0010858
Physical Location & Seq
Forward (+)
64747232 .. 64752363
5132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG448300.1

Sequence Viewer

Length: 717 bp
ATGTACACCGATTTGTTCTGGTTTTCAAGGTTGGGGATCGGCTGCACAGCTAAACCAGAGAGTGACTGTTGGAGTGACGATAGTGACAGCGATAAGAAATCTGTTTCTTTTAGCAATACTATTGCCAGCACATATGCTACTTCAGATGATTCTGTTTCTACCAGTGGCCATGAGATTGAGGCCGATTGCCTTCACCACCTGTATTGCCAGTACAACGAAACATCTAGTCCTCATGATAGAGTTCCTTTGACCCTCAAGATCAATGAATTAGCTCAAATTTATCCTGGTTTGATGGAGTTTCAAAGCTTGGAGCTCTCTCCTTGTAGCTGGATGGCTGTTGCTTGGTATCCTATCTATCAAATTCCAGTTACAAGGAATGTGAAGGAATTATCTGCATATTTCATCACTTATCACACATTATCATCTTTTCAAGGCCTCCATAGTACCATATCAGAGAATAATGATAAGTTTCAATGCATTGATACATTGGGAAAGGATCATGGAAGCAAGACGCTAGAAAGAGATGAAAGCAAAGGTGAAATGGCTCTTCCTCCTTTTGCAGCGGCCACTTATAAGATGCATGGCGAACTTTGGACAACTCCTGAGACTTCGGATAAGGAAAAGATTGGCTTTTATCTGAACGCTGCAAATTCCTGGTTGAAGCATCGCAAATTCCGGCATCATGACTTTATTTCTTCATATCTCGTAGGTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF789

Protein Analysis

238

Amino Acids

27.05

Weight (kDa)

5.39

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF789 PF05623 19 - 230 1.9e-56 Protein of unknown function (DUF789)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 573
AciI CCGC 1 cut(s) 563
AclWI GGATC 2 cut(s) 44, 504
AcoI YGGCCR 2 cut(s) 166, 564
AcsI RAATTY 4 cut(s) 276, 360, 649, 671
AcuI CTGAAG 1 cut(s) 126
AfaI GTAC 3 cut(s) 5, 212, 445
AgsI TTSAA 5 cut(s) 27, 302, 431, 473, 661
AjnI CCWGG 2 cut(s) 283, 653
AluBI AGCT 5 cut(s) 50, 272, 306, 313, 327
AluI AGCT 5 cut(s) 50, 272, 306, 313, 327
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 1 cut(s) 599
AlwI GGATC 2 cut(s) 44, 504
AoxI GGCC 4 cut(s) 166, 180, 433, 564
ApeKI GCWGC 3 cut(s) 42, 560, 644
ApoI RAATTY 4 cut(s) 276, 360, 649, 671
AsuHPI GGTGA 2 cut(s) 185, 548
BalI TGGCCA 1 cut(s) 168
BanII GRGCYC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 3 cut(s) 29, 572, 631
BccI CCATC 2 cut(s) 286, 325
BciT130I CCWGG 2 cut(s) 285, 655
BciVI GTATCC 1 cut(s) 357
BcoDI GTCTC 1 cut(s) 599
BfaI CTAG 2 cut(s) 225, 515
BfuI GTATCC 1 cut(s) 357
BisI GCNGC 4 cut(s) 43, 561, 564, 645
BlsI GCNGC 4 cut(s) 44, 562, 565, 646
Bme1390I CCNGG 2 cut(s) 285, 655
BmrFI CCNGG 2 cut(s) 285, 655
BmsI GCATC 3 cut(s) 567, 673, 688
BpuEI CTTGAG 1 cut(s) 239
Bse1I ACTGG 3 cut(s) 162, 208, 365
BseBI CCWGG 2 cut(s) 285, 655
BseGI GGATG 1 cut(s) 336
BseMII CTCAG 1 cut(s) 594
BseNI ACTGG 3 cut(s) 162, 208, 365
BseXI GCAGC 3 cut(s) 29, 572, 631
BsgI GTGCAG 1 cut(s) 28
BshFI GGCC 4 cut(s) 168, 182, 435, 566
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 1 cut(s) 676
BsmAI GTCTC 1 cut(s) 599
BsnI GGCC 4 cut(s) 168, 182, 435, 566
Bsp1286I GDGCHC 1 cut(s) 315
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 36, 258, 496
BspACI CCGC 1 cut(s) 563
BspANI GGCC 4 cut(s) 168, 182, 435, 566
BspCNI CTCAG 1 cut(s) 595
BspHI TCATGA 2 cut(s) 232, 682
BspPI GGATC 2 cut(s) 44, 504
BspQI GCTCTTC 1 cut(s) 552
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 3 cut(s) 162, 208, 365
BssMI GATC 3 cut(s) 36, 258, 496
Bst2UI CCWGG 2 cut(s) 285, 655
Bst4CI ACNGT 1 cut(s) 68
Bst6I CTCTTC 1 cut(s) 552
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 1 cut(s) 127
BstDEI CTNAG 1 cut(s) 603
BstF5I GGATG 1 cut(s) 336
BstKTI GATC 3 cut(s) 39, 261, 499
BstMAI GTCTC 1 cut(s) 599
BstMBI GATC 3 cut(s) 36, 258, 496
BstNI CCWGG 2 cut(s) 285, 655
BstSCI CCNGG 2 cut(s) 283, 653
BstV1I GCAGC 3 cut(s) 29, 572, 631
BsuI GTATCC 1 cut(s) 357
BsuRI GGCC 4 cut(s) 168, 182, 435, 566
BtgZI GCGATG 1 cut(s) 650
BtsCI GGATG 1 cut(s) 336
BtsIMutI CAGTG 1 cut(s) 169
Cac8I GCNNGC 1 cut(s) 127
CciI TCATGA 2 cut(s) 232, 682
CseI GACGC 1 cut(s) 520
Csp6I GTAC 3 cut(s) 4, 211, 444
CspCI CAANNNNNGTGG 2 cut(s) 185, 220
CviAII CATG 5 cut(s) 170, 233, 500, 581, 683
CviQI GTAC 3 cut(s) 4, 211, 444
DdeI CTNAG 1 cut(s) 603
DpnI GATC 3 cut(s) 38, 260, 498
DpnII GATC 3 cut(s) 36, 258, 496
EaeI YGGCCR 2 cut(s) 166, 564
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
Ecl136II GAGCTC 1 cut(s) 313
Eco147I AGGCCT 1 cut(s) 435
Eco24I GRGCYC 1 cut(s) 315
Eco53kI GAGCTC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 126
EcoICRI GAGCTC 1 cut(s) 313
EcoRII CCWGG 2 cut(s) 283, 653
EcoT22I ATGCAT 2 cut(s) 479, 582
EcoT38I GRGCYC 1 cut(s) 315
FaeI CATG 5 cut(s) 173, 236, 503, 584, 686
FalI AAGNNNNNCTT 2 cut(s) 614, 646
FatI CATG 5 cut(s) 169, 232, 499, 580, 682
FauNDI CATATG 1 cut(s) 133
Fnu4HI GCNGC 4 cut(s) 43, 561, 564, 645
FokI GGATG 1 cut(s) 343
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 4 cut(s) 43, 561, 564, 645
FspBI CTAG 2 cut(s) 225, 515
GluI GCNGC 4 cut(s) 43, 561, 564, 645
HaeIII GGCC 4 cut(s) 168, 182, 435, 566
HapII CCGG 1 cut(s) 676
HgaI GACGC 1 cut(s) 520
Hin1II CATG 5 cut(s) 173, 236, 503, 584, 686
HindIII AAGCTT 1 cut(s) 304
HinfI GANTC 1 cut(s) 149
HpaII CCGG 1 cut(s) 676
HphI GGTGA 2 cut(s) 185, 548
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 4 cut(s) 145, 454, 613, 639
Hpy188III TCNNGA 4 cut(s) 233, 256, 602, 683
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 2 cut(s) 200, 376
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4V TGCA 6 cut(s) 45, 395, 477, 560, 580, 647
HpyF3I CTNAG 1 cut(s) 603
Hsp92II CATG 5 cut(s) 173, 236, 503, 584, 686
Kzo9I GATC 3 cut(s) 36, 258, 496
LguI GCTCTTC 1 cut(s) 552
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 3 cut(s) 29, 572, 631
LweI GCATC 3 cut(s) 567, 673, 688
MaeI CTAG 2 cut(s) 225, 515
MaeIII GTNAC 5 cut(s) 62, 74, 83, 367, 710
MalI GATC 3 cut(s) 38, 260, 498
MboI GATC 3 cut(s) 36, 258, 496
MboII GAAGA 2 cut(s) 539, 687
MhlI GDGCHC 1 cut(s) 315
MlsI TGGCCA 1 cut(s) 168
MluCI AATT 6 cut(s) 266, 276, 360, 386, 649, 671
MluNI TGGCCA 1 cut(s) 168
MmeI TCCRAC 1 cut(s) 50
MnlI CCTC 5 cut(s) 172, 240, 263, 446, 561
Mox20I TGGCCA 1 cut(s) 168
Mph1103I ATGCAT 2 cut(s) 479, 582
MscI TGGCCA 1 cut(s) 168
Msp20I TGGCCA 1 cut(s) 168
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 1 cut(s) 676
MspR9I CCNGG 2 cut(s) 285, 655
MvaI CCWGG 2 cut(s) 285, 655
NdeI CATATG 1 cut(s) 133
NdeII GATC 3 cut(s) 36, 258, 496
NlaIII CATG 5 cut(s) 173, 236, 503, 584, 686
NmuCI GTSAC 3 cut(s) 62, 74, 83
NsiI ATGCAT 2 cut(s) 479, 582
PagI TCATGA 2 cut(s) 232, 682
PceI AGGCCT 1 cut(s) 435
PciSI GCTCTTC 1 cut(s) 552
PfeI GAWTC 1 cut(s) 149
PkrI GCNGC 4 cut(s) 44, 562, 565, 646
PsiI TTATAA 1 cut(s) 573
Psp124BI GAGCTC 1 cut(s) 315
Psp6I CCWGG 2 cut(s) 283, 653
PspGI CCWGG 2 cut(s) 283, 653
RsaI GTAC 3 cut(s) 5, 212, 445
RsaNI GTAC 3 cut(s) 4, 211, 444
SacI GAGCTC 1 cut(s) 315
SapI GCTCTTC 1 cut(s) 552
SatI GCNGC 4 cut(s) 43, 561, 564, 645
Sau3AI GATC 3 cut(s) 36, 258, 496
ScrFI CCNGG 2 cut(s) 285, 655
SduI GDGCHC 1 cut(s) 315
SetI ASST 9 cut(s) 32, 52, 201, 274, 308, 315, 329, 538, 712
SfaNI GCATC 3 cut(s) 567, 673, 688
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 6 cut(s) 266, 276, 360, 386, 649, 671
SseBI AGGCCT 1 cut(s) 435
SsiI CCGC 1 cut(s) 563
SspMI CTAG 2 cut(s) 225, 515
SstI GAGCTC 1 cut(s) 315
StuI AGGCCT 1 cut(s) 435
StyD4I CCNGG 2 cut(s) 283, 653
TaaI ACNGT 1 cut(s) 68
TasI AATT 6 cut(s) 266, 276, 360, 386, 649, 671
TatI WGTACW 2 cut(s) 3, 210
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 1 cut(s) 149
TscAI CASTG 1 cut(s) 169
TseFI GTSAC 3 cut(s) 62, 74, 83
TseI GCWGC 3 cut(s) 42, 560, 644
Tsp45I GTSAC 3 cut(s) 62, 74, 83
TspDTI ATGAA 4 cut(s) 279, 391, 540, 687
TspRI CASTG 1 cut(s) 169
XapI RAATTY 4 cut(s) 276, 360, 649, 671
XspI CTAG 2 cut(s) 225, 515
Zsp2I ATGCAT 2 cut(s) 479, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.