Rh4AG440800

Protein of unknown function (DUF789)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0010858
Physical Location & Seq
Forward (+)
73196413 .. 73202258
5846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG440800.1

Sequence Viewer

Length: 561 bp
ATGTACACCGATTTGTTCTGGTTTTCAAGGTTGGGGATCGGCTGCACAGCTAAACCAGAGAGTGACTGTTGGAGTGACGATAGTGACAGCGATAAGAAAGCTGTTTCTTTTAGCAATACAATTGCCAGCACATATGCTACTTCAGATGATTCTGTTTCTACCAGTGGCCATGAGATTGAGGCCGATTGCCTTCACCACCTGTATTGCCAGTACAACGAAACATCTAGTCCTCATGATAGAGTTCCTTTGACCCTCAAGATCAATGAATTAGCTCAAATTTATCCTGGTTTGATGGAGTTTCAAAGCTTGGAGCTCTCTCCTTGTAGCTGGATGGCTGTTGCTTGGTATCCTATCTATCAAATTCCAGTTACAAGGAATGTGAAGGAATTATCTGCATATTTCATCACTTATCACACATTATCATCTTTTCAAGCATACGATGCAAATTTATTGGCAACAGTTTCAGATGCTCATAACATGGGAGGTTTACCAGGTCTATTGTGTCCTTGTAGGATGCAAGGTGTGCCCTCGAGAGTTTACCAACATAAGATAGCTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF789

Protein Analysis

186

Amino Acids

20.81

Weight (kDa)

5.05

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF789 PF05623 19 - 144 8.6e-39 Protein of unknown function (DUF789)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 44
AcoI YGGCCR 1 cut(s) 166
AcsI RAATTY 3 cut(s) 276, 360, 445
AcuI CTGAAG 1 cut(s) 126
AfaI GTAC 2 cut(s) 5, 212
AgsI TTSAA 3 cut(s) 27, 302, 431
AjnI CCWGG 2 cut(s) 283, 490
AluBI AGCT 7 cut(s) 50, 101, 272, 306, 313, 327, 554
AluI AGCT 7 cut(s) 50, 101, 272, 306, 313, 327, 554
Alw21I GWGCWC 1 cut(s) 315
AlwI GGATC 1 cut(s) 44
Ama87I CYCGRG 1 cut(s) 529
AoxI GGCC 2 cut(s) 166, 180
ApeKI GCWGC 1 cut(s) 42
ApoI RAATTY 3 cut(s) 276, 360, 445
AsuHPI GGTGA 1 cut(s) 185
AvaI CYCGRG 1 cut(s) 529
BaeGI GKGCMC 1 cut(s) 528
BalI TGGCCA 1 cut(s) 168
BanII GRGCYC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 1 cut(s) 29
BccI CCATC 2 cut(s) 286, 325
BciT130I CCWGG 2 cut(s) 285, 492
BciVI GTATCC 1 cut(s) 357
BfaI CTAG 1 cut(s) 225
BfuI GTATCC 1 cut(s) 357
BisI GCNGC 1 cut(s) 43
BlsI GCNGC 1 cut(s) 44
Bme1390I CCNGG 2 cut(s) 285, 492
BmeT110I CYCGRG 1 cut(s) 529
BmrFI CCNGG 2 cut(s) 285, 492
BmsI GCATC 3 cut(s) 430, 457, 504
BpuEI CTTGAG 1 cut(s) 239
Bse1I ACTGG 3 cut(s) 162, 208, 365
BseBI CCWGG 2 cut(s) 285, 492
BseGI GGATG 2 cut(s) 336, 519
BseNI ACTGG 3 cut(s) 162, 208, 365
BseSI GKGCMC 1 cut(s) 528
BseXI GCAGC 1 cut(s) 29
BsgI GTGCAG 1 cut(s) 28
BshFI GGCC 2 cut(s) 168, 182
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 529
BsnI GGCC 2 cut(s) 168, 182
BsoBI CYCGRG 1 cut(s) 529
Bsp1286I GDGCHC 2 cut(s) 315, 528
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 36, 258
BspANI GGCC 2 cut(s) 168, 182
BspHI TCATGA 1 cut(s) 232
BspPI GGATC 1 cut(s) 44
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 3 cut(s) 162, 208, 365
BssMI GATC 2 cut(s) 36, 258
Bst2UI CCWGG 2 cut(s) 285, 492
Bst4CI ACNGT 2 cut(s) 68, 460
BstAPI GCANNNNNTGC 2 cut(s) 440, 523
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 1 cut(s) 127
BstF5I GGATG 2 cut(s) 336, 519
BstKTI GATC 2 cut(s) 39, 261
BstMBI GATC 2 cut(s) 36, 258
BstMWI GCNNNNNNNGC 2 cut(s) 440, 523
BstNI CCWGG 2 cut(s) 285, 492
BstSCI CCNGG 2 cut(s) 283, 490
BstSLI GKGCMC 1 cut(s) 528
BstV1I GCAGC 1 cut(s) 29
BsuI GTATCC 1 cut(s) 357
BsuRI GGCC 2 cut(s) 168, 182
BtsCI GGATG 2 cut(s) 336, 519
BtsIMutI CAGTG 1 cut(s) 169
Cac8I GCNNGC 1 cut(s) 127
CciI TCATGA 1 cut(s) 232
CsiI ACCWGGT 1 cut(s) 490
Csp6I GTAC 2 cut(s) 4, 211
CspCI CAANNNNNGTGG 2 cut(s) 185, 220
CviAII CATG 3 cut(s) 170, 233, 478
CviQI GTAC 2 cut(s) 4, 211
DpnI GATC 2 cut(s) 38, 260
DpnII GATC 2 cut(s) 36, 258
EaeI YGGCCR 1 cut(s) 166
Ecl136II GAGCTC 1 cut(s) 313
Eco24I GRGCYC 1 cut(s) 315
Eco53kI GAGCTC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 529
EcoICRI GAGCTC 1 cut(s) 313
EcoRII CCWGG 2 cut(s) 283, 490
EcoT38I GRGCYC 1 cut(s) 315
FaeI CATG 3 cut(s) 173, 236, 481
FaiI YATR 9 cut(s) 133, 135, 171, 234, 397, 436, 474, 479, 546
FatI CATG 3 cut(s) 169, 232, 477
FauNDI CATATG 1 cut(s) 133
Fnu4HI GCNGC 1 cut(s) 43
FokI GGATG 2 cut(s) 343, 526
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 1 cut(s) 43
FspBI CTAG 1 cut(s) 225
GluI GCNGC 1 cut(s) 43
HaeIII GGCC 2 cut(s) 168, 182
Hin1II CATG 3 cut(s) 173, 236, 481
HindIII AAGCTT 1 cut(s) 304
HinfI GANTC 1 cut(s) 149
HphI GGTGA 1 cut(s) 185
Hpy166II GTNNAC 3 cut(s) 6, 488, 538
Hpy188I TCNGA 2 cut(s) 145, 466
Hpy188III TCNNGA 3 cut(s) 233, 256, 531
Hpy8I GTNNAC 3 cut(s) 6, 488, 538
HpyAV CCTTC 2 cut(s) 200, 376
HpyCH4III ACNGT 2 cut(s) 68, 460
HpyCH4V TGCA 4 cut(s) 45, 395, 443, 517
HpyF10VI GCNNNNNNNGC 2 cut(s) 440, 523
Hsp92II CATG 3 cut(s) 173, 236, 481
Kzo9I GATC 2 cut(s) 36, 258
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 3 cut(s) 430, 457, 504
MabI ACCWGGT 1 cut(s) 490
MaeI CTAG 1 cut(s) 225
MaeIII GTNAC 4 cut(s) 62, 74, 83, 367
MalI GATC 2 cut(s) 38, 260
MboI GATC 2 cut(s) 36, 258
MfeI CAATTG 1 cut(s) 120
MhlI GDGCHC 2 cut(s) 315, 528
MlsI TGGCCA 1 cut(s) 168
MluCI AATT 6 cut(s) 120, 266, 276, 360, 386, 445
MluNI TGGCCA 1 cut(s) 168
MmeI TCCRAC 1 cut(s) 50
MnlI CCTC 5 cut(s) 172, 240, 263, 476, 538
Mox20I TGGCCA 1 cut(s) 168
MscI TGGCCA 1 cut(s) 168
MseI TTAA 1 cut(s) 559
Msp20I TGGCCA 1 cut(s) 168
MspR9I CCNGG 2 cut(s) 285, 492
MunI CAATTG 1 cut(s) 120
MvaI CCWGG 2 cut(s) 285, 492
MwoI GCNNNNNNNGC 2 cut(s) 440, 523
NdeI CATATG 1 cut(s) 133
NdeII GATC 2 cut(s) 36, 258
NlaIII CATG 3 cut(s) 173, 236, 481
NmuCI GTSAC 3 cut(s) 62, 74, 83
PaeR7I CTCGAG 1 cut(s) 529
PagI TCATGA 1 cut(s) 232
PfeI GAWTC 1 cut(s) 149
PkrI GCNGC 1 cut(s) 44
Psp124BI GAGCTC 1 cut(s) 315
Psp6I CCWGG 2 cut(s) 283, 490
PspGI CCWGG 2 cut(s) 283, 490
RsaI GTAC 2 cut(s) 5, 212
RsaNI GTAC 2 cut(s) 4, 211
SacI GAGCTC 1 cut(s) 315
SaqAI TTAA 1 cut(s) 559
SatI GCNGC 1 cut(s) 43
Sau3AI GATC 2 cut(s) 36, 258
ScrFI CCNGG 2 cut(s) 285, 492
SduI GDGCHC 2 cut(s) 315, 528
SexAI ACCWGGT 1 cut(s) 490
SfaNI GCATC 3 cut(s) 430, 457, 504
Sfr274I CTCGAG 1 cut(s) 529
SlaI CTCGAG 1 cut(s) 529
SmlI CTYRAG 2 cut(s) 254, 529
SmoI CTYRAG 2 cut(s) 254, 529
Sse9I AATT 6 cut(s) 120, 266, 276, 360, 386, 445
SspMI CTAG 1 cut(s) 225
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 2 cut(s) 283, 490
TaaI ACNGT 2 cut(s) 68, 460
TaqI TCGA 1 cut(s) 530
TasI AATT 6 cut(s) 120, 266, 276, 360, 386, 445
TatI WGTACW 2 cut(s) 3, 210
TfiI GAWTC 1 cut(s) 149
Tru1I TTAA 1 cut(s) 559
Tru9I TTAA 1 cut(s) 559
TscAI CASTG 1 cut(s) 169
TseFI GTSAC 3 cut(s) 62, 74, 83
TseI GCWGC 1 cut(s) 42
Tsp45I GTSAC 3 cut(s) 62, 74, 83
TspDTI ATGAA 2 cut(s) 279, 391
TspRI CASTG 1 cut(s) 169
XapI RAATTY 3 cut(s) 276, 360, 445
XhoI CTCGAG 1 cut(s) 529
XspI CTAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.