Rh4BG449900

Protein of unknown function (DUF789)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0010858
Physical Location & Seq
Forward (+)
58770707 .. 58772820
2114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG449900.1

Sequence Viewer

Length: 480 bp
ATGTACACCGATTTGTTCTGGTTTTCAAGGTTGGGGATCGGCTGCACAGCTAAACCAGAGAGTGACTGTTGGAGTGACGATAGTGACAGCGATAAGAAATCTGTTTGTTTTAGCAATACTACTGCCAGCACATATGCTACTTCAGATGATTCTGTTTCTACCAGTGACCATGAGATTGAGGCCGATTGCCTTCACCACCTGTATTGCCACTACAACGAAACATCTAGTCCTCATGATAGAGTTCCTTTGACCCTCAAGATCAATGAATTAGCTCAAATTTATCCTGGTTTGATGGAGTTCCAAAGCTTGGAGCTCTCTCCTTATAGCTGGATGGCTGTTGCTTGGTATCCTATCTATCAAATTCCAGTTACAAGGAATGTGAAGGAATTATCTGCATGTTTCATCACTTATCACACATTATCATCTTTTCAAGGTATAATGGCTACTATTTCTTCGTCTCCCTTGTTTGGTAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF789

Protein Analysis

159

Amino Acids

17.96

Weight (kDa)

4.66

Isoelectric Point (pI)

54.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF789 PF05623 19 - 143 2.7e-40 Protein of unknown function (DUF789)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 307
AclWI GGATC 1 cut(s) 44
AcsI RAATTY 2 cut(s) 276, 360
AcuI CTGAAG 1 cut(s) 126
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 307, 467
AgsI TTSAA 2 cut(s) 27, 431
AjnI CCWGG 1 cut(s) 283
AluBI AGCT 6 cut(s) 50, 272, 306, 313, 327, 474
AluI AGCT 6 cut(s) 50, 272, 306, 313, 327, 474
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 1 cut(s) 462
AlwI GGATC 1 cut(s) 44
AoxI GGCC 1 cut(s) 180
ApeKI GCWGC 1 cut(s) 42
ApoI RAATTY 2 cut(s) 276, 360
AsuHPI GGTGA 1 cut(s) 185
BanII GRGCYC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 1 cut(s) 29
BccI CCATC 2 cut(s) 286, 325
BciT130I CCWGG 1 cut(s) 285
BciVI GTATCC 1 cut(s) 357
BcoDI GTCTC 1 cut(s) 462
BfaI CTAG 1 cut(s) 225
BfuI GTATCC 1 cut(s) 357
BisI GCNGC 1 cut(s) 43
BlsI GCNGC 1 cut(s) 44
Bme1390I CCNGG 1 cut(s) 285
BmrFI CCNGG 1 cut(s) 285
BpuEI CTTGAG 1 cut(s) 239
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 467
Bse1I ACTGG 2 cut(s) 162, 365
BseBI CCWGG 1 cut(s) 285
BseGI GGATG 1 cut(s) 336
BseLI CCNNNNNNNGG 2 cut(s) 307, 467
BseNI ACTGG 2 cut(s) 162, 365
BseXI GCAGC 1 cut(s) 29
BsgI GTGCAG 1 cut(s) 28
BshFI GGCC 1 cut(s) 182
BsiHKAI GWGCWC 1 cut(s) 315
BslI CCNNNNNNNGG 2 cut(s) 307, 467
BsmAI GTCTC 1 cut(s) 462
BsmBI CGTCTC 1 cut(s) 462
BsnI GGCC 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 315
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 36, 258
BspANI GGCC 1 cut(s) 182
BspHI TCATGA 1 cut(s) 232
BspPI GGATC 1 cut(s) 44
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 2 cut(s) 162, 365
BssMI GATC 2 cut(s) 36, 258
Bst2UI CCWGG 1 cut(s) 285
Bst4CI ACNGT 1 cut(s) 68
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 1 cut(s) 127
BstF5I GGATG 1 cut(s) 336
BstKTI GATC 2 cut(s) 39, 261
BstMAI GTCTC 1 cut(s) 462
BstMBI GATC 2 cut(s) 36, 258
BstNI CCWGG 1 cut(s) 285
BstNSI RCATGY 1 cut(s) 399
BstSCI CCNGG 1 cut(s) 283
BstV1I GCAGC 1 cut(s) 29
BsuI GTATCC 1 cut(s) 357
BsuRI GGCC 1 cut(s) 182
BtsCI GGATG 1 cut(s) 336
BtsIMutI CAGTG 1 cut(s) 169
Cac8I GCNNGC 1 cut(s) 127
CciI TCATGA 1 cut(s) 232
Csp6I GTAC 1 cut(s) 4
CspCI CAANNNNNGTGG 2 cut(s) 185, 220
CviAII CATG 3 cut(s) 170, 233, 396
CviQI GTAC 1 cut(s) 4
DpnI GATC 2 cut(s) 38, 260
DpnII GATC 2 cut(s) 36, 258
Ecl136II GAGCTC 1 cut(s) 313
Eco24I GRGCYC 1 cut(s) 315
Eco53kI GAGCTC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 126
EcoICRI GAGCTC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 315
Esp3I CGTCTC 1 cut(s) 462
FaeI CATG 3 cut(s) 173, 236, 399
FaiI YATR 7 cut(s) 133, 135, 171, 234, 324, 397, 437
FatI CATG 3 cut(s) 169, 232, 395
FauNDI CATATG 1 cut(s) 133
Fnu4HI GCNGC 1 cut(s) 43
FokI GGATG 1 cut(s) 343
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 1 cut(s) 43
FspBI CTAG 1 cut(s) 225
GluI GCNGC 1 cut(s) 43
HaeIII GGCC 1 cut(s) 182
Hin1II CATG 3 cut(s) 173, 236, 399
HindIII AAGCTT 1 cut(s) 304
HinfI GANTC 1 cut(s) 149
HphI GGTGA 1 cut(s) 185
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 2 cut(s) 233, 256
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 2 cut(s) 200, 376
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4V TGCA 2 cut(s) 45, 395
Hsp92II CATG 3 cut(s) 173, 236, 399
Kzo9I GATC 2 cut(s) 36, 258
LmnI GCTCC 1 cut(s) 310
LpnPI CCDG 9 cut(s) 4, 69, 139, 175, 212, 270, 297, 313, 378
Lsp1109I GCAGC 1 cut(s) 29
MaeI CTAG 1 cut(s) 225
MaeIII GTNAC 5 cut(s) 62, 74, 83, 164, 367
MalI GATC 2 cut(s) 38, 260
MboI GATC 2 cut(s) 36, 258
MboII GAAGA 1 cut(s) 444
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 4 cut(s) 266, 276, 360, 386
MmeI TCCRAC 1 cut(s) 50
MnlI CCTC 3 cut(s) 172, 240, 263
MspR9I CCNGG 1 cut(s) 285
MvaI CCWGG 1 cut(s) 285
NdeI CATATG 1 cut(s) 133
NdeII GATC 2 cut(s) 36, 258
NlaIII CATG 3 cut(s) 173, 236, 399
NmuCI GTSAC 4 cut(s) 62, 74, 83, 164
NspI RCATGY 1 cut(s) 399
PagI TCATGA 1 cut(s) 232
PfeI GAWTC 1 cut(s) 149
PflMI CCANNNNNTGG 1 cut(s) 307
PkrI GCNGC 1 cut(s) 44
Psp124BI GAGCTC 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 283
PspGI CCWGG 1 cut(s) 283
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SacI GAGCTC 1 cut(s) 315
SatI GCNGC 1 cut(s) 43
Sau3AI GATC 2 cut(s) 36, 258
ScrFI CCNGG 1 cut(s) 285
SduI GDGCHC 1 cut(s) 315
SetI ASST 9 cut(s) 32, 52, 201, 274, 308, 315, 329, 436, 476
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 4 cut(s) 266, 276, 360, 386
SspMI CTAG 1 cut(s) 225
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 1 cut(s) 283
TaaI ACNGT 1 cut(s) 68
TasI AATT 4 cut(s) 266, 276, 360, 386
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 1 cut(s) 149
TscAI CASTG 1 cut(s) 169
TseFI GTSAC 4 cut(s) 62, 74, 83, 164
TseI GCWGC 1 cut(s) 42
Tsp45I GTSAC 4 cut(s) 62, 74, 83, 164
TspDTI ATGAA 2 cut(s) 279, 391
TspRI CASTG 1 cut(s) 169
Van91I CCANNNNNTGG 1 cut(s) 307
XapI RAATTY 2 cut(s) 276, 360
XceI RCATGY 1 cut(s) 399
XspI CTAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.