RchiOBHm_Chr2g0094361

Thermospermine synthase ACAULIS5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004438
Physical Location & Seq
Reverse (-)
7304156 .. 7305505
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 537 bp
ATGGAAAGCTGCAGAGTGCTGAAAATGATGCCCCAAAACCATATTCATAATGGTGAAGCGTCAACAGCAAGGGAGGCACTGAAGCACAAAGATGTGGAGAAAGTTATCATGTGTGATATAGATGAGGTGATTGTCAATTTTTGCCGCGCACATCTGGAAGAAAATCAACTAGGCTTCAACTTGTTTTCAATGATCCTAAGTGAGGCTGAGTTGGAGAAAACTGAAGAGAAGTTTGATGTGATTGTGGGAGACCTACCAGACCCAATTGAGGGAGGCCCTTGCAATAATCTTTACACCAAAAACTTTTATGAGCAAGTCATAAAACCTCACCTCAAAGACAATGGCATCTTTGTCACTCAAGCTGGTCCAGCAGGAATTCTTTCTCATAAGGCTATCTTCACTTCAATAGACAAACACTGTAAAACAAGTGTTCAAATATGTGTTGGCATACACAGCTCATGTGCCATCTTATGCAGATTCATGTGGATGGGTGATGGCTTCAGATCAACCACTCAGATTGGATGCTGCACAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spermine_synt_N Spermine_synth

Protein Analysis

178

Amino Acids

19.91

Weight (kDa)

5.72

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synth PF01564 20 - 146 7e-21 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 147
AciI CCGC 1 cut(s) 145
AclWI GGATC 1 cut(s) 187
AcsI RAATTY 1 cut(s) 375
AcuI CTGAAG 3 cut(s) 101, 243, 484
AfiI CCNNNNNNNGG 3 cut(s) 202, 268, 269
AgsI TTSAA 4 cut(s) 178, 189, 405, 434
AjuI GAANNNNNNNTTGG 2 cut(s) 27, 59
AluBI AGCT 3 cut(s) 9, 362, 456
AluI AGCT 3 cut(s) 9, 362, 456
Alw26I GTCTC 1 cut(s) 243
AlwI GGATC 1 cut(s) 187
AoxI GGCC 1 cut(s) 274
ApeKI GCWGC 2 cut(s) 9, 525
ApoI RAATTY 1 cut(s) 375
Asp700I GAANNNNTTC 1 cut(s) 379
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 2 cut(s) 275, 365
AsuHPI GGTGA 4 cut(s) 65, 139, 320, 503
AvaII GGWCC 1 cut(s) 365
BbvI GCAGC 1 cut(s) 512
BccI CCATC 3 cut(s) 473, 481, 488
BcoDI GTCTC 1 cut(s) 243
BfaI CTAG 1 cut(s) 170
BfmI CTRYAG 1 cut(s) 10
BisI GCNGC 3 cut(s) 10, 145, 526
BlsI GCNGC 3 cut(s) 11, 146, 527
Bme18I GGWCC 1 cut(s) 365
BmgT120I GGNCC 2 cut(s) 275, 365
BmsI GCATC 3 cut(s) 18, 354, 512
BpuEI CTTGAG 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 243
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 268, 269
BseGI GGATG 2 cut(s) 492, 527
BseLI CCNNNNNNNGG 3 cut(s) 202, 268, 269
BseMII CTCAG 2 cut(s) 198, 527
BseXI GCAGC 1 cut(s) 512
BsgI GTGCAG 1 cut(s) 511
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 1 cut(s) 276
BslI CCNNNNNNNGG 3 cut(s) 202, 268, 269
BsmAI GTCTC 1 cut(s) 243
BsnI GGCC 1 cut(s) 276
Bso31I GGTCTC 1 cut(s) 243
Bsp143I GATC 2 cut(s) 192, 503
BspACI CCGC 1 cut(s) 145
BspANI GGCC 1 cut(s) 276
BspCNI CTCAG 2 cut(s) 199, 526
BspFNI CGCG 1 cut(s) 147
BspMAI CTGCAG 1 cut(s) 14
BspPI GGATC 1 cut(s) 187
BspTNI GGTCTC 1 cut(s) 243
BssMI GATC 2 cut(s) 192, 503
Bst4CI ACNGT 1 cut(s) 419
Bst6I CTCTTC 1 cut(s) 219
BstDEI CTNAG 3 cut(s) 197, 207, 513
BstENI CCTNNNNNAGG 1 cut(s) 200
BstF5I GGATG 2 cut(s) 492, 527
BstFNI CGCG 1 cut(s) 147
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 2 cut(s) 195, 506
BstMAI GTCTC 1 cut(s) 243
BstMBI GATC 2 cut(s) 192, 503
BstMWI GCNNNNNNNGC 4 cut(s) 65, 74, 368, 453
BstSFI CTRYAG 1 cut(s) 10
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 1 cut(s) 512
BsuRI GGCC 1 cut(s) 276
BtsCI GGATG 2 cut(s) 492, 527
BtsIMutI CAGTG 2 cut(s) 77, 415
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 275, 365
CseI GACGC 1 cut(s) 48
CspCI CAANNNNNGTGG 2 cut(s) 499, 534
CviAII CATG 3 cut(s) 109, 459, 481
CviJI RGCY 8 cut(s) 9, 174, 206, 276, 362, 392, 456, 498
CviKI_1 RGCY 8 cut(s) 9, 174, 206, 276, 362, 392, 456, 498
DdeI CTNAG 3 cut(s) 197, 207, 513
DpnI GATC 2 cut(s) 194, 505
DpnII GATC 2 cut(s) 192, 503
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Eco31I GGTCTC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 365
Eco57I CTGAAG 3 cut(s) 101, 243, 484
EcoNI CCTNNNNNAGG 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 275
EcoRI GAATTC 1 cut(s) 375
FaeI CATG 3 cut(s) 112, 462, 484
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FatI CATG 3 cut(s) 108, 458, 480
Fnu4HI GCNGC 3 cut(s) 10, 145, 526
FokI GGATG 1 cut(s) 499
Fsp4HI GCNGC 3 cut(s) 10, 145, 526
FspBI CTAG 1 cut(s) 170
GlaI GCGC 1 cut(s) 148
GluI GCNGC 3 cut(s) 10, 145, 526
HaeIII GGCC 1 cut(s) 276
HgaI GACGC 1 cut(s) 48
HhaI GCGC 1 cut(s) 149
Hin1II CATG 3 cut(s) 112, 462, 484
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HinfI GANTC 1 cut(s) 477
HphI GGTGA 4 cut(s) 65, 139, 320, 503
Hpy166II GTNNAC 1 cut(s) 63
Hpy188I TCNGA 2 cut(s) 503, 516
Hpy188III TCNNGA 1 cut(s) 155
Hpy8I GTNNAC 1 cut(s) 63
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4V TGCA 4 cut(s) 12, 282, 474, 528
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 74, 368, 453
HpyF3I CTNAG 3 cut(s) 197, 207, 513
Hsp92II CATG 3 cut(s) 112, 462, 484
HspAI GCGC 1 cut(s) 147
Kzo9I GATC 2 cut(s) 192, 503
LpnPI CCDG 5 cut(s) 140, 270, 348, 357, 381
Lsp1109I GCAGC 1 cut(s) 512
LweI GCATC 3 cut(s) 18, 354, 512
MaeI CTAG 1 cut(s) 170
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 194, 505
MboI GATC 2 cut(s) 192, 503
MboII GAAGA 3 cut(s) 170, 236, 388
MfeI CAATTG 1 cut(s) 264
MluCI AATT 3 cut(s) 136, 264, 375
MmeI TCCRAC 1 cut(s) 192
MnlI CCTC 7 cut(s) 67, 118, 196, 262, 266, 336, 341
MroXI GAANNNNTTC 1 cut(s) 379
MslI CAYNNNNRTG 3 cut(s) 51, 90, 485
MunI CAATTG 1 cut(s) 264
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 4 cut(s) 65, 74, 368, 453
NdeII GATC 2 cut(s) 192, 503
NlaIII CATG 3 cut(s) 112, 462, 484
NmuCI GTSAC 1 cut(s) 352
PdmI GAANNNNTTC 1 cut(s) 379
PfeI GAWTC 1 cut(s) 477
PkrI GCNGC 3 cut(s) 11, 146, 527
PspPI GGNCC 2 cut(s) 275, 365
PstI CTGCAG 1 cut(s) 14
RseI CAYNNNNRTG 3 cut(s) 51, 90, 485
SatI GCNGC 3 cut(s) 10, 145, 526
Sau3AI GATC 2 cut(s) 192, 503
Sau96I GGNCC 2 cut(s) 275, 365
SetI ASST 7 cut(s) 11, 129, 255, 328, 333, 364, 458
SfaNI GCATC 3 cut(s) 18, 354, 512
SfcI CTRYAG 1 cut(s) 10
SinI GGWCC 1 cut(s) 365
SmiMI CAYNNNNRTG 3 cut(s) 51, 90, 485
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
Sse9I AATT 3 cut(s) 136, 264, 375
SsiI CCGC 1 cut(s) 145
SspMI CTAG 1 cut(s) 170
TaaI ACNGT 1 cut(s) 419
TasI AATT 3 cut(s) 136, 264, 375
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 1 cut(s) 477
TscAI CASTG 2 cut(s) 84, 422
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 2 cut(s) 9, 525
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 2 cut(s) 35, 469
TspRI CASTG 2 cut(s) 84, 422
VpaK11BI GGWCC 1 cut(s) 365
XagI CCTNNNNNAGG 1 cut(s) 200
XapI RAATTY 1 cut(s) 375
XcmI CCANNNNNNNNNTGG 1 cut(s) 47
XmnI GAANNNNTTC 1 cut(s) 379
XspI CTAG 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.