RchiOBHm_Chr2g0110191

lactoylglutathione

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010618
Physical Location & Seq
Forward (+)
21692394 .. 21692801
408 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 408 bp
ATGAGGATATTCGTTGCTTCGGAGCTTCTAGAATTGCCCAAGAAGGACAAGCGCCGCCTCCTCCATGCCGTGTACTGCGTCGGTGATCTCGAGCGCACCATCAAGTTTCACACTGAGGCTCTTGGGATGAAGCTGTTGTGGCAGAGGGATATTCCGGACGAGAAGTACTCGAATGCTTTCCTCGAATTCGGGCCGGAAGAAACGAACTTTGTAATGGAGCTCACTTACAATTATGGAGTAATTCCTATGATATTGGGATTGGTTTTGGTTACAAAAGAGCTTGGAGGAAAGATAACAAGAGAGTCGGTAAAGGACTGGGAAAATTTCTGGTCACAGCCATGGAGGAAGAGAGGGAGAGTGTCAAAGTCAGAAAGAGGTACAAGTACTAAATTACCCTCTGACAAGTGA

Protein Analysis

135

Amino Acids

15.78

Weight (kDa)

9.21

Isoelectric Point (pI)

23.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 19 - 67 1.8e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 154
AciI CCGC 1 cut(s) 55
AcsI RAATTY 2 cut(s) 185, 322
AfaI GTAC 4 cut(s) 74, 167, 379, 385
AluBI AGCT 4 cut(s) 25, 133, 220, 280
AluI AGCT 4 cut(s) 25, 133, 220, 280
Alw21I GWGCWC 1 cut(s) 222
Ama87I CYCGRG 1 cut(s) 89
Aor13HI TCCGGA 1 cut(s) 154
AoxI GGCC 1 cut(s) 191
ApoI RAATTY 2 cut(s) 185, 322
Asp700I GAANNNNTTC 1 cut(s) 176
AspLEI GCGC 2 cut(s) 54, 96
AspS9I GGNCC 1 cut(s) 191
AsuHPI GGTGA 1 cut(s) 95
AvaI CYCGRG 1 cut(s) 89
BanII GRGCYC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 222
BccI CCATC 1 cut(s) 107
BceAI ACGGC 1 cut(s) 53
BfaI CTAG 1 cut(s) 29
BfoI RGCGCY 1 cut(s) 55
BisI GCNGC 1 cut(s) 55
BlsI GCNGC 1 cut(s) 56
BmcAI AGTACT 2 cut(s) 167, 385
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 191
BmrI ACTGGG 1 cut(s) 325
BmuI ACTGGG 1 cut(s) 325
BplI GAGNNNNNCTC 2 cut(s) 152, 184
BsaJI CCNNGG 1 cut(s) 338
BsaWI WCCGGW 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 320
BseAI TCCGGA 1 cut(s) 154
BseDI CCNNGG 1 cut(s) 338
BseGI GGATG 1 cut(s) 132
BseMII CTCAG 1 cut(s) 105
BseNI ACTGG 1 cut(s) 320
BseRI GAGGAG 1 cut(s) 50
BshFI GGCC 1 cut(s) 193
BsiHKAI GWGCWC 1 cut(s) 222
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 2 cut(s) 155, 194
BsmI GAATGC 1 cut(s) 178
BsnI GGCC 1 cut(s) 193
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 222
Bsp13I TCCGGA 1 cut(s) 154
Bsp143I GATC 1 cut(s) 85
Bsp19I CCATGG 1 cut(s) 338
BspACI CCGC 1 cut(s) 55
BspANI GGCC 1 cut(s) 193
BspCNI CTCAG 1 cut(s) 106
BspEI TCCGGA 1 cut(s) 154
BsrI ACTGG 1 cut(s) 320
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 1 cut(s) 85
BssT1I CCWWGG 1 cut(s) 338
Bst6I CTCTTC 1 cut(s) 341
BstDEI CTNAG 1 cut(s) 114
BstDSI CCRYGG 1 cut(s) 338
BstF5I GGATG 1 cut(s) 132
BstH2I RGCGCY 1 cut(s) 55
BstHHI GCGC 2 cut(s) 54, 96
BstKTI GATC 1 cut(s) 88
BstMBI GATC 1 cut(s) 85
BstMWI GCNNNNNNNGC 1 cut(s) 139
BsuRI GGCC 1 cut(s) 193
BtgI CCRYGG 1 cut(s) 338
BtsCI GGATG 1 cut(s) 132
BtsIMutI CAGTG 1 cut(s) 111
CfoI GCGC 2 cut(s) 54, 96
Cfr13I GGNCC 1 cut(s) 191
CseI GACGC 1 cut(s) 67
Csp6I GTAC 4 cut(s) 73, 166, 378, 384
CviAII CATG 2 cut(s) 65, 339
CviJI RGCY 7 cut(s) 25, 119, 133, 193, 220, 280, 337
CviKI_1 RGCY 7 cut(s) 25, 119, 133, 193, 220, 280, 337
CviQI GTAC 4 cut(s) 73, 166, 378, 384
DdeI CTNAG 1 cut(s) 114
DpnI GATC 1 cut(s) 87
DpnII GATC 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 341
EarI CTCTTC 1 cut(s) 341
Ecl136II GAGCTC 1 cut(s) 220
Eco130I CCWWGG 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 222
Eco53kI GAGCTC 1 cut(s) 220
Eco88I CYCGRG 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 220
EcoRI GAATTC 1 cut(s) 185
EcoT14I CCWWGG 1 cut(s) 338
EcoT38I GRGCYC 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 338
FaeI CATG 2 cut(s) 68, 342
FaiI YATR 4 cut(s) 66, 234, 248, 340
FatI CATG 2 cut(s) 64, 338
Fnu4HI GCNGC 1 cut(s) 55
FokI GGATG 1 cut(s) 139
FriOI GRGCYC 1 cut(s) 222
Fsp4HI GCNGC 1 cut(s) 55
FspBI CTAG 1 cut(s) 29
GlaI GCGC 2 cut(s) 53, 95
GluI GCNGC 1 cut(s) 55
HaeII RGCGCY 1 cut(s) 55
HaeIII GGCC 1 cut(s) 193
HapII CCGG 2 cut(s) 155, 194
HgaI GACGC 1 cut(s) 67
HhaI GCGC 2 cut(s) 54, 96
Hin1II CATG 2 cut(s) 68, 342
Hin6I GCGC 2 cut(s) 52, 94
HinP1I GCGC 2 cut(s) 52, 94
HinfI GANTC 1 cut(s) 302
HpaII CCGG 2 cut(s) 155, 194
HphI GGTGA 1 cut(s) 95
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 3 cut(s) 22, 370, 400
Hpy188III TCNNGA 3 cut(s) 29, 89, 155
Hpy8I GTNNAC 1 cut(s) 73
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 1 cut(s) 37
HpyF10VI GCNNNNNNNGC 1 cut(s) 139
HpyF3I CTNAG 1 cut(s) 114
Hsp92II CATG 2 cut(s) 68, 342
HspAI GCGC 2 cut(s) 52, 94
Kpn2I TCCGGA 1 cut(s) 154
Kzo9I GATC 1 cut(s) 85
LmnI GCTCC 2 cut(s) 22, 217
LpnPI CCDG 4 cut(s) 168, 207, 301, 313
MaeI CTAG 1 cut(s) 29
MaeIII GTNAC 2 cut(s) 268, 330
MalI GATC 1 cut(s) 87
MboI GATC 1 cut(s) 85
MboII GAAGA 2 cut(s) 209, 358
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 6 cut(s) 32, 185, 229, 240, 322, 389
MlyI GAGTC 1 cut(s) 311
MroI TCCGGA 1 cut(s) 154
MroXI GAANNNNTTC 1 cut(s) 176
MslI CAYNNNNRTG 1 cut(s) 337
MspI CCGG 2 cut(s) 155, 194
Mva1269I GAATGC 1 cut(s) 178
MwoI GCNNNNNNNGC 1 cut(s) 139
NcoI CCATGG 1 cut(s) 338
NdeII GATC 1 cut(s) 85
NlaIII CATG 2 cut(s) 68, 342
NmuCI GTSAC 1 cut(s) 330
PaeR7I CTCGAG 1 cut(s) 89
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PctI GAATGC 1 cut(s) 178
PdmI GAANNNNTTC 1 cut(s) 176
PkrI GCNGC 1 cut(s) 56
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
Psp124BI GAGCTC 1 cut(s) 222
PspPI GGNCC 1 cut(s) 191
RsaI GTAC 4 cut(s) 74, 167, 379, 385
RsaNI GTAC 4 cut(s) 73, 166, 378, 384
RseI CAYNNNNRTG 1 cut(s) 337
SacI GAGCTC 1 cut(s) 222
SatI GCNGC 1 cut(s) 55
Sau3AI GATC 1 cut(s) 85
Sau96I GGNCC 1 cut(s) 191
ScaI AGTACT 2 cut(s) 167, 385
SchI GAGTC 1 cut(s) 311
SduI GDGCHC 1 cut(s) 222
SetI ASST 5 cut(s) 27, 135, 222, 282, 379
Sfr274I CTCGAG 1 cut(s) 89
SlaI CTCGAG 1 cut(s) 89
SmiMI CAYNNNNRTG 1 cut(s) 337
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
Sse9I AATT 6 cut(s) 32, 185, 229, 240, 322, 389
SsiI CCGC 1 cut(s) 55
SspMI CTAG 1 cut(s) 29
SstI GAGCTC 1 cut(s) 222
StyI CCWWGG 1 cut(s) 338
TaqI TCGA 3 cut(s) 90, 170, 183
TasI AATT 6 cut(s) 32, 185, 229, 240, 322, 389
TatI WGTACW 3 cut(s) 72, 165, 383
TauI GCSGC 1 cut(s) 57
TscAI CASTG 1 cut(s) 118
TseFI GTSAC 1 cut(s) 330
Tsp45I GTSAC 1 cut(s) 330
TspDTI ATGAA 1 cut(s) 143
TspRI CASTG 1 cut(s) 118
XapI RAATTY 2 cut(s) 185, 322
XbaI TCTAGA 1 cut(s) 28
XhoI CTCGAG 1 cut(s) 89
XmnI GAANNNNTTC 1 cut(s) 176
XspI CTAG 1 cut(s) 29
ZrmI AGTACT 2 cut(s) 167, 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.