RchiOBHm_Chr2g0132781

Pentatricopeptide repeat-containing protein At1g80880

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007278
Physical Location & Seq
Forward (+)
49376347 .. 49378080
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1200 bp
ATGGCACTTCCCTCCATAGCAAGAAGGCTACGAACTAAACACCCCCTCTGCCTATCACTCACTCTACTCCACCACCACCACCACCACCCCATCACTAACTCACCCTATTCTTCGCTTTCTCTGCACCCCCGTCTCTCTCAGCCATTTCATCCAACATCTATCGTTCCATCCCAAACCCCTCGCCATTTCTCTACTCTCCGACCCTTCTCCGCCGAAATCCCCCACCACCCATTGGGCTTCAATGGCCAGCGTTTCAACACAGACCACTCCCACAACTTGGGTCTCACCCAGTTTCTTGAATTGCTTACAAGTACGGCCGGTTTGGCCTCTGAAGCCGACGCCGTGGCTTTTCTCGACGAGTCGGGCGTCGAGCCGAAGCGAGATACAGTCTTTCAGGCGATTTGGGAGCTGAGGGGAGATTGGAAGTTGGCGTTTTTGGGTTTCAAATGGGGTGAGAAGTGGGGCTGTTGCGATGAAGAGGCGTGTAGTTTGATGGTTTGGGTTTTCGGGAGTCACCGAAAGTTTAGTACTGCTTGGTGTTTGATACGGGACTTGAATCAGGCTTTGATTGATACGCGCCGCGCAATGCTTATAATGATTGATAGATATGCATCTGCAAATCATCCAAGGAAGGCCATTAAGACATTTCAGATAATGGAGAAGTTCAGGTTGAGCCCTGATCAAGAAGCATTCCACATCCTCTTGAATGCACTTTGTAAAAATGGAAACATGGAGGAAGCTGAAGAATTCATGCTTGTAAGTAAGAAGTTCTTCCCACTGGAGGTCGAGGGCTTTAACATTGTTCTCAATGGGTGGTGTAACATATCTGTTGATGTATATCAAGCCAAGAGAGTTTGGAGAGAAATGTCTAAATACTGTATTACACCAGACGCAACTTCTTATACACACATGATTTCCTGCTTCTCAAAGGTTGGGAATCTTTTCGACTCCCTTAGACTATATGATGAAATGAAGAAAAGGGATTGGGTTCCTGGTCTCAAGGTTTACAATTCTTTAATTTATGTACTAACCCGTGAAAATTGCTTTAAGGAAGCACTCAAAGTACTGGACAAAGTGAAAGAAATTGGTTTGCAGCCAGATGCTACCACCTATAATTCAATGATAGGTCCTTTATGTGAATCAAAGAAGATAGAGGAGGCGCAACAGATGCTGAAGTCCAGGTTCAAAGGCAACTGTTGA

Protein Analysis

399

Amino Acids

45.87

Weight (kDa)

8.63

Isoelectric Point (pI)

34.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 198 - 240 1.7e-07 PPR repeat family
PPR_2 PF13041 264 - 310 3.1e-06 PPR repeat family
PPR_3 PF13812 284 - 343 2.2e-06 Pentatricopeptide repeat domain
PPR PF01535 300 - 327 6.5e-06 PPR repeat
PPR_2 PF13041 334 - 379 2.8e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 593
AccB7I CCANNNNNTGG 2 cut(s) 232, 277
AccII CGCG 2 cut(s) 577, 582
AciI CCGC 2 cut(s) 210, 580
AcoI YGGCCR 2 cut(s) 244, 315
AcsI RAATTY 1 cut(s) 746
AcuI CTGAAG 3 cut(s) 351, 762, 1193
AcyI GRCGYC 2 cut(s) 339, 366
AfaI GTAC 4 cut(s) 313, 529, 1026, 1065
AfiI CCNNNNNNNGG 3 cut(s) 232, 277, 781
AgsI TTSAA 8 cut(s) 241, 256, 299, 445, 556, 706, 1119, 1186
AjnI CCWGG 2 cut(s) 991, 1178
AluBI AGCT 2 cut(s) 409, 740
AluI AGCT 2 cut(s) 409, 740
Alw26I GTCTC 3 cut(s) 137, 287, 1001
AlwNI CAGNNNCTG 1 cut(s) 1171
AoxI GGCC 4 cut(s) 244, 315, 324, 633
ApeKI GCWGC 1 cut(s) 1093
ApoI RAATTY 1 cut(s) 746
Asp700I GAANNNNTTC 2 cut(s) 770, 941
AspLEI GCGC 3 cut(s) 579, 584, 1162
AspS9I GGNCC 1 cut(s) 1127
AsuHPI GGTGA 4 cut(s) 93, 277, 464, 506
AvaII GGWCC 1 cut(s) 1127
BalI TGGCCA 1 cut(s) 246
BanII GRGCYC 1 cut(s) 677
BbvCI CCTCAGC 1 cut(s) 410
BbvI GCAGC 1 cut(s) 1105
BccI CCATC 3 cut(s) 98, 175, 487
BceAI ACGGC 2 cut(s) 326, 330
BciT130I CCWGG 2 cut(s) 993, 1180
BclI TGATCA 1 cut(s) 679
BcoDI GTCTC 3 cut(s) 137, 287, 1001
BglI GCCNNNNNGGC 1 cut(s) 323
BisI GCNGC 2 cut(s) 580, 1094
BlsI GCNGC 2 cut(s) 581, 1095
BmcAI AGTACT 2 cut(s) 529, 1065
Bme1390I CCNGG 2 cut(s) 993, 1180
Bme18I GGWCC 1 cut(s) 1127
BmgT120I GGNCC 1 cut(s) 1127
BmiI GGNNCC 1 cut(s) 990
BmrFI CCNGG 2 cut(s) 993, 1180
BmrI ACTGGG 1 cut(s) 283
BmsI GCATC 3 cut(s) 620, 1090, 1158
BmuI ACTGGG 1 cut(s) 283
BpmI CTGGAG 1 cut(s) 800
Bpu10I CCTNAGC 1 cut(s) 410
BpuEI CTTGAG 1 cut(s) 983
BsaBI GATNNNNATC 2 cut(s) 610, 837
BsaHI GRCGYC 2 cut(s) 339, 366
BsaI GGTCTC 2 cut(s) 287, 1001
BsaJI CCNNGG 2 cut(s) 342, 626
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 277, 781
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 3 cut(s) 289, 783, 1071
Bse3DI GCAATG 1 cut(s) 591
Bse8I GATNNNNATC 2 cut(s) 610, 837
BseBI CCWGG 2 cut(s) 993, 1180
BseDI CCNNGG 2 cut(s) 342, 626
BseGI GGATG 4 cut(s) 148, 167, 622, 696
BseJI GATNNNNATC 2 cut(s) 610, 837
BseLI CCNNNNNNNGG 3 cut(s) 232, 277, 781
BseMI GCAATG 1 cut(s) 591
BseMII CTCAG 2 cut(s) 152, 401
BseNI ACTGG 3 cut(s) 289, 783, 1071
BseRI GAGGAG 1 cut(s) 1169
BseX3I CGGCCG 1 cut(s) 315
BseXI GCAGC 1 cut(s) 1105
BsgI GTGCAG 1 cut(s) 107
Bsh1236I CGCG 2 cut(s) 577, 582
Bsh1285I CGRYCG 1 cut(s) 318
BshFI GGCC 4 cut(s) 246, 317, 326, 635
BsiEI CGRYCG 1 cut(s) 318
BsiSI CCGG 1 cut(s) 318
BslFI GGGAC 1 cut(s) 563
BslI CCNNNNNNNGG 3 cut(s) 232, 277, 781
BsmAI GTCTC 3 cut(s) 137, 287, 1001
BsmBI CGTCTC 1 cut(s) 137
BsmFI GGGAC 1 cut(s) 563
BsmI GAATGC 2 cut(s) 689, 712
BsnI GGCC 4 cut(s) 246, 317, 326, 635
Bso31I GGTCTC 2 cut(s) 287, 1001
Bsp1286I GDGCHC 1 cut(s) 677
Bsp143I GATC 1 cut(s) 679
BspACI CCGC 2 cut(s) 210, 580
BspANI GGCC 4 cut(s) 246, 317, 326, 635
BspCNI CTCAG 2 cut(s) 151, 402
BspFNI CGCG 2 cut(s) 577, 582
BspLI GGNNCC 1 cut(s) 990
BspTNI GGTCTC 2 cut(s) 287, 1001
BsrDI GCAATG 1 cut(s) 591
BsrFI RCCGGY 1 cut(s) 317
BsrI ACTGG 3 cut(s) 289, 783, 1071
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 2 cut(s) 342, 626
BssMI GATC 1 cut(s) 679
BssNI GRCGYC 2 cut(s) 339, 366
BssT1I CCWWGG 1 cut(s) 626
Bst2UI CCWGG 2 cut(s) 993, 1180
Bst4CI ACNGT 3 cut(s) 388, 878, 1196
Bst6I CTCTTC 1 cut(s) 471
BstACI GRCGYC 2 cut(s) 339, 366
BstAPI GCANNNNNTGC 1 cut(s) 1168
BstC8I GCNNGC 1 cut(s) 248
BstDEI CTNAG 3 cut(s) 138, 410, 953
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 4 cut(s) 148, 167, 622, 696
BstFNI CGCG 2 cut(s) 577, 582
BstHHI GCGC 3 cut(s) 579, 584, 1162
BstKTI GATC 1 cut(s) 682
BstMAI GTCTC 3 cut(s) 137, 287, 1001
BstMBI GATC 1 cut(s) 679
BstMCI CGRYCG 1 cut(s) 318
BstMWI GCNNNNNNNGC 5 cut(s) 121, 243, 323, 332, 1168
BstNI CCWGG 2 cut(s) 993, 1180
BstSCI CCNGG 2 cut(s) 991, 1178
BstUI CGCG 2 cut(s) 577, 582
BstV1I GCAGC 1 cut(s) 1105
BstZI CGGCCG 1 cut(s) 315
BsuRI GGCC 4 cut(s) 246, 317, 326, 635
BtgI CCRYGG 1 cut(s) 342
BtgZI GCGATG 1 cut(s) 486
BtsCI GGATG 4 cut(s) 148, 167, 622, 696
BtsIMutI CAGTG 1 cut(s) 776
Cac8I GCNNGC 1 cut(s) 248
CaiI CAGNNNCTG 1 cut(s) 1171
CfoI GCGC 3 cut(s) 579, 584, 1162
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 1 cut(s) 1127
CseI GACGC 3 cut(s) 347, 355, 899
Csp6I GTAC 4 cut(s) 312, 528, 1025, 1064
CviAII CATG 3 cut(s) 730, 751, 910
CviQI GTAC 4 cut(s) 312, 528, 1025, 1064
DdeI CTNAG 3 cut(s) 138, 410, 953
DpnI GATC 1 cut(s) 681
DpnII GATC 1 cut(s) 679
EaeI YGGCCR 2 cut(s) 244, 315
EagI CGGCCG 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
EciI GGCGGA 1 cut(s) 199
EclXI CGGCCG 1 cut(s) 315
Eco130I CCWWGG 1 cut(s) 626
Eco24I GRGCYC 1 cut(s) 677
Eco31I GGTCTC 2 cut(s) 287, 1001
Eco47I GGWCC 1 cut(s) 1127
Eco52I CGGCCG 1 cut(s) 315
Eco57I CTGAAG 3 cut(s) 351, 762, 1193
EcoO109I RGGNCCY 1 cut(s) 1127
EcoRI GAATTC 1 cut(s) 746
EcoRII CCWGG 2 cut(s) 991, 1178
EcoT14I CCWWGG 1 cut(s) 626
EcoT22I ATGCAT 1 cut(s) 613
EcoT38I GRGCYC 1 cut(s) 677
ErhI CCWWGG 1 cut(s) 626
Esp3I CGTCTC 1 cut(s) 137
FaeI CATG 3 cut(s) 733, 754, 913
FalI AAGNNNNNCTT 2 cut(s) 755, 787
FaqI GGGAC 1 cut(s) 563
FatI CATG 3 cut(s) 729, 750, 909
FbaI TGATCA 1 cut(s) 679
Fnu4HI GCNGC 2 cut(s) 580, 1094
FokI GGATG 4 cut(s) 135, 154, 609, 683
FriOI GRGCYC 1 cut(s) 677
Fsp4HI GCNGC 2 cut(s) 580, 1094
GlaI GCGC 3 cut(s) 578, 583, 1161
GluI GCNGC 2 cut(s) 580, 1094
GsuI CTGGAG 1 cut(s) 800
HaeIII GGCC 4 cut(s) 246, 317, 326, 635
HapII CCGG 1 cut(s) 318
HgaI GACGC 3 cut(s) 347, 355, 899
HhaI GCGC 3 cut(s) 579, 584, 1162
Hin1I GRCGYC 2 cut(s) 339, 366
Hin1II CATG 3 cut(s) 733, 754, 913
Hin6I GCGC 3 cut(s) 577, 582, 1160
HinP1I GCGC 3 cut(s) 577, 582, 1160
HinfI GANTC 6 cut(s) 359, 511, 556, 937, 947, 1139
HpaII CCGG 1 cut(s) 318
HphI GGTGA 4 cut(s) 93, 277, 464, 506
Hpy166II GTNNAC 1 cut(s) 1006
Hpy188I TCNGA 3 cut(s) 200, 331, 651
Hpy188III TCNNGA 5 cut(s) 296, 353, 508, 683, 703
Hpy8I GTNNAC 1 cut(s) 1006
Hpy99I CGWCG 3 cut(s) 341, 359, 371
HpyAV CCTTC 3 cut(s) 18, 214, 625
HpyCH4III ACNGT 3 cut(s) 388, 878, 1196
HpyCH4V TGCA 5 cut(s) 124, 611, 617, 710, 1093
HpyF10VI GCNNNNNNNGC 5 cut(s) 121, 243, 323, 332, 1168
HpyF3I CTNAG 3 cut(s) 138, 410, 953
Hsp92I GRCGYC 2 cut(s) 339, 366
Hsp92II CATG 3 cut(s) 733, 754, 913
HspAI GCGC 3 cut(s) 577, 582, 1160
Ksp22I TGATCA 1 cut(s) 679
Kzo9I GATC 1 cut(s) 679
LmnI GCTCC 1 cut(s) 406
Lsp1109I GCAGC 1 cut(s) 1105
LweI GCATC 3 cut(s) 620, 1090, 1158
MaeIII GTNAC 2 cut(s) 512, 818
MalI GATC 1 cut(s) 681
MboI GATC 1 cut(s) 679
MboII GAAGA 6 cut(s) 102, 488, 755, 763, 985, 1159
MhlI GDGCHC 1 cut(s) 677
MlsI TGGCCA 1 cut(s) 246
MluCI AATT 7 cut(s) 299, 746, 1009, 1017, 1039, 1083, 1114
MluNI TGGCCA 1 cut(s) 246
MlyI GAGTC 3 cut(s) 368, 520, 941
MmeI TCCRAC 2 cut(s) 176, 223
Mox20I TGGCCA 1 cut(s) 246
Mph1103I ATGCAT 1 cut(s) 613
MroXI GAANNNNTTC 2 cut(s) 770, 941
MscI TGGCCA 1 cut(s) 246
MseI TTAA 4 cut(s) 639, 795, 1016, 1047
Msp20I TGGCCA 1 cut(s) 246
MspI CCGG 1 cut(s) 318
MspR9I CCNGG 2 cut(s) 993, 1180
MteI GCGCNGCGC 1 cut(s) 580
Mva1269I GAATGC 2 cut(s) 689, 712
MvaI CCWGG 2 cut(s) 993, 1180
MvnI CGCG 2 cut(s) 577, 582
MwoI GCNNNNNNNGC 5 cut(s) 121, 243, 323, 332, 1168
NdeII GATC 1 cut(s) 679
NlaIII CATG 3 cut(s) 733, 754, 913
NlaIV GGNNCC 1 cut(s) 990
NmuCI GTSAC 1 cut(s) 512
NsiI ATGCAT 1 cut(s) 613
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PctI GAATGC 2 cut(s) 689, 712
PdmI GAANNNNTTC 2 cut(s) 770, 941
PfeI GAWTC 3 cut(s) 556, 937, 1139
PflMI CCANNNNNTGG 2 cut(s) 232, 277
PkrI GCNGC 2 cut(s) 581, 1095
PleI GAGTC 3 cut(s) 367, 519, 941
PpsI GAGTC 3 cut(s) 367, 519, 941
PpuMI RGGWCCY 1 cut(s) 1127
PsiI TTATAA 1 cut(s) 593
Psp5II RGGWCCY 1 cut(s) 1127
Psp6I CCWGG 2 cut(s) 991, 1178
PspGI CCWGG 2 cut(s) 991, 1178
PspN4I GGNNCC 1 cut(s) 990
PspPI GGNCC 1 cut(s) 1127
PspPPI RGGWCCY 1 cut(s) 1127
PstNI CAGNNNCTG 1 cut(s) 1171
RsaI GTAC 4 cut(s) 313, 529, 1026, 1065
RsaNI GTAC 4 cut(s) 312, 528, 1025, 1064
SaqAI TTAA 4 cut(s) 639, 795, 1016, 1047
SatI GCNGC 2 cut(s) 580, 1094
Sau3AI GATC 1 cut(s) 679
Sau96I GGNCC 1 cut(s) 1127
ScaI AGTACT 2 cut(s) 529, 1065
SchI GAGTC 3 cut(s) 368, 520, 941
ScrFI CCNGG 2 cut(s) 993, 1180
SduI GDGCHC 1 cut(s) 677
SetI ASST 9 cut(s) 411, 671, 742, 786, 933, 1005, 1112, 1129, 1184
SfaNI GCATC 3 cut(s) 620, 1090, 1158
SfiI GGCCNNNNNGGCC 1 cut(s) 323
SinI GGWCC 1 cut(s) 1127
SmlI CTYRAG 1 cut(s) 998
SmoI CTYRAG 1 cut(s) 998
Sse9I AATT 7 cut(s) 299, 746, 1009, 1017, 1039, 1083, 1114
SsiI CCGC 2 cut(s) 210, 580
StyD4I CCNGG 2 cut(s) 991, 1178
StyI CCWWGG 1 cut(s) 626
TaaI ACNGT 3 cut(s) 388, 878, 1196
TaqI TCGA 4 cut(s) 354, 369, 786, 945
TasI AATT 7 cut(s) 299, 746, 1009, 1017, 1039, 1083, 1114
TatI WGTACW 3 cut(s) 527, 1024, 1063
TauI GCSGC 1 cut(s) 582
TfiI GAWTC 3 cut(s) 556, 937, 1139
Tru1I TTAA 4 cut(s) 639, 795, 1016, 1047
Tru9I TTAA 4 cut(s) 639, 795, 1016, 1047
TscAI CASTG 1 cut(s) 783
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 1 cut(s) 1093
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 5 cut(s) 137, 489, 739, 981, 986
TspRI CASTG 1 cut(s) 783
Van91I CCANNNNNTGG 2 cut(s) 232, 277
VpaK11BI GGWCC 1 cut(s) 1127
XapI RAATTY 1 cut(s) 746
XmnI GAANNNNTTC 2 cut(s) 770, 941
ZrmI AGTACT 2 cut(s) 529, 1065
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.