RchiOBHm_Chr2g0153331

Golgi to ER traffic protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008175
Physical Location & Seq
Forward (+)
70678178 .. 70681039
2862 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 417 bp
ATGTCGAAGGAGAGAGCCAAAAGAGGCACACTGCCTCCAGCCCAAGAGAAAGTTGTAGAGGAAGGTAACTACCATGGAGCTCAGCAGCTGTACAAATCTATTAGTGCGAGATATGTAGCTGCTCAGAGGTATACTGAAGCTTTAGATATTCTTCAGTCGGGTGCATGCATCCAGTTGAAACATGGGCAGGTTACGTGTGGGGCAGAGTTAGCTGCTTTGTTTGTGGATACACTTGTCAAAGCGAGATGCCCTTATGACGATGAAACCCTCGGTCAGTTGCTCGCCTCCAGCGTCAAGCACCGCCTCCCTCCAGTCCATCCTTCCCTCTTCTCTGTTGCCTCTGCTGGAGCTCCTTCAGTCTATTTCTTCCATCAAGGCCGATTACATTGCTCGCCAACAGTGCAAAGCATCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF410

Protein Analysis

138

Amino Acids

15.02

Weight (kDa)

8.72

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GET4 PF04190 42 - 94 1.1e-12 Golgi to ER traffic protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 178
AccI GTMKAC 1 cut(s) 131
AciI CCGC 1 cut(s) 301
AcuI CTGAAG 3 cut(s) 137, 156, 339
AfaI GTAC 1 cut(s) 92
AflIII ACRYGT 1 cut(s) 194
AgsI TTSAA 1 cut(s) 178
AluBI AGCT 6 cut(s) 80, 88, 119, 140, 212, 350
AluI AGCT 6 cut(s) 80, 88, 119, 140, 212, 350
Alw21I GWGCWC 2 cut(s) 82, 352
AlwNI CAGNNNCTG 1 cut(s) 88
AoxI GGCC 1 cut(s) 376
ApeKI GCWGC 3 cut(s) 85, 119, 212
BaeI ACNNNNGTAYC 2 cut(s) 219, 252
BanII GRGCYC 2 cut(s) 82, 352
Bbv12I GWGCWC 2 cut(s) 82, 352
BbvI GCAGC 3 cut(s) 97, 106, 199
BccI CCATC 2 cut(s) 324, 378
BcgI CGANNNNNNTGC 2 cut(s) 369, 403
BciVI GTATCC 1 cut(s) 220
BfaI CTAG 1 cut(s) 415
BfuAI ACCTGC 1 cut(s) 178
BfuI GTATCC 1 cut(s) 220
BisI GCNGC 3 cut(s) 86, 120, 213
BlpI GCTNAGC 1 cut(s) 81
BlsI GCNGC 3 cut(s) 87, 121, 214
BmsI GCATC 2 cut(s) 177, 236
BpmI CTGGAG 4 cut(s) 21, 271, 294, 366
Bpu1102I GCTNAGC 1 cut(s) 81
BsaAI YACGTR 1 cut(s) 195
BsaJI CCNNGG 2 cut(s) 73, 268
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bse1I ACTGG 2 cut(s) 172, 311
Bse3DI GCAATG 1 cut(s) 385
BseDI CCNNGG 2 cut(s) 73, 268
BseGI GGATG 3 cut(s) 168, 316, 408
BseMI GCAATG 1 cut(s) 385
BseMII CTCAG 2 cut(s) 95, 137
BseNI ACTGG 2 cut(s) 172, 311
BseXI GCAGC 3 cut(s) 97, 106, 199
BshFI GGCC 1 cut(s) 378
BsiHKAI GWGCWC 2 cut(s) 82, 352
BsnI GGCC 1 cut(s) 378
Bsp1286I GDGCHC 2 cut(s) 82, 352
Bsp1407I TGTACA 1 cut(s) 90
Bsp1720I GCTNAGC 1 cut(s) 81
Bsp19I CCATGG 1 cut(s) 73
BspACI CCGC 1 cut(s) 301
BspANI GGCC 1 cut(s) 378
BspCNI CTCAG 2 cut(s) 94, 136
BspMI ACCTGC 1 cut(s) 178
BsrDI GCAATG 1 cut(s) 385
BsrGI TGTACA 1 cut(s) 90
BsrI ACTGG 2 cut(s) 172, 311
BssECI CCNNGG 2 cut(s) 73, 268
BssNAI GTATAC 1 cut(s) 132
BssT1I CCWWGG 1 cut(s) 73
Bst1107I GTATAC 1 cut(s) 132
Bst4CI ACNGT 1 cut(s) 400
Bst6I CTCTTC 1 cut(s) 332
BstAUI TGTACA 1 cut(s) 90
BstBAI YACGTR 1 cut(s) 195
BstC8I GCNNGC 3 cut(s) 166, 282, 392
BstDEI CTNAG 2 cut(s) 81, 123
BstDSI CCRYGG 1 cut(s) 73
BstF5I GGATG 3 cut(s) 168, 316, 408
BstMWI GCNNNNNNNGC 2 cut(s) 209, 400
BstNSI RCATGY 1 cut(s) 168
BstV1I GCAGC 3 cut(s) 97, 106, 199
BstZ17I GTATAC 1 cut(s) 132
BsuI GTATCC 1 cut(s) 220
BsuRI GGCC 1 cut(s) 378
BtgI CCRYGG 1 cut(s) 73
BtsCI GGATG 3 cut(s) 168, 316, 408
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 2 cut(s) 29, 405
BveI ACCTGC 1 cut(s) 178
Cac8I GCNNGC 3 cut(s) 166, 282, 392
CaiI CAGNNNCTG 1 cut(s) 88
CseI GACGC 1 cut(s) 280
Csp6I GTAC 1 cut(s) 91
CviAII CATG 3 cut(s) 74, 165, 182
CviJI RGCY 9 cut(s) 17, 41, 80, 88, 119, 140, 212, 350, 378
CviKI_1 RGCY 9 cut(s) 17, 41, 80, 88, 119, 140, 212, 350, 378
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 2 cut(s) 81, 123
Eam1104I CTCTTC 1 cut(s) 332
EarI CTCTTC 1 cut(s) 332
Ecl136II GAGCTC 2 cut(s) 80, 350
Eco130I CCWWGG 1 cut(s) 73
Eco24I GRGCYC 2 cut(s) 82, 352
Eco53kI GAGCTC 2 cut(s) 80, 350
Eco57I CTGAAG 3 cut(s) 137, 156, 339
EcoICRI GAGCTC 2 cut(s) 80, 350
EcoT14I CCWWGG 1 cut(s) 73
EcoT22I ATGCAT 1 cut(s) 170
EcoT38I GRGCYC 2 cut(s) 82, 352
ErhI CCWWGG 1 cut(s) 73
FaeI CATG 3 cut(s) 77, 168, 185
FaiI YATR 6 cut(s) 75, 114, 132, 166, 183, 255
FatI CATG 3 cut(s) 73, 164, 181
FblI GTMKAC 1 cut(s) 131
Fnu4HI GCNGC 3 cut(s) 86, 120, 213
FokI GGATG 3 cut(s) 155, 303, 395
FriOI GRGCYC 2 cut(s) 82, 352
Fsp4HI GCNGC 3 cut(s) 86, 120, 213
FspBI CTAG 1 cut(s) 415
GluI GCNGC 3 cut(s) 86, 120, 213
GsuI CTGGAG 4 cut(s) 21, 271, 294, 366
HaeIII GGCC 1 cut(s) 378
HgaI GACGC 1 cut(s) 280
Hin1II CATG 3 cut(s) 77, 168, 185
HindIII AAGCTT 1 cut(s) 138
Hpy166II GTNNAC 1 cut(s) 132
Hpy188I TCNGA 1 cut(s) 126
Hpy8I GTNNAC 1 cut(s) 132
HpyAV CCTTC 3 cut(s) 56, 330, 363
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4IV ACGT 1 cut(s) 194
HpyCH4V TGCA 3 cut(s) 164, 168, 403
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 400
HpyF3I CTNAG 2 cut(s) 81, 123
HpySE526I ACGT 1 cut(s) 194
Hsp92II CATG 3 cut(s) 77, 168, 185
LmnI GCTCC 3 cut(s) 77, 347, 355
LpnPI CCDG 6 cut(s) 51, 173, 185, 301, 324, 330
Lsp1109I GCAGC 3 cut(s) 97, 106, 199
LweI GCATC 2 cut(s) 177, 236
MaeI CTAG 1 cut(s) 415
MaeII ACGT 1 cut(s) 194
MaeIII GTNAC 2 cut(s) 65, 190
MboII GAAGA 3 cut(s) 143, 319, 358
MhlI GDGCHC 2 cut(s) 82, 352
Mph1103I ATGCAT 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 88
MwoI GCNNNNNNNGC 2 cut(s) 209, 400
NcoI CCATGG 1 cut(s) 73
NlaIII CATG 3 cut(s) 77, 168, 185
NsiI ATGCAT 1 cut(s) 170
NspI RCATGY 1 cut(s) 168
PaeI GCATGC 1 cut(s) 168
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PkrI GCNGC 3 cut(s) 87, 121, 214
Ppu21I YACGTR 1 cut(s) 195
Psp124BI GAGCTC 2 cut(s) 82, 352
PstNI CAGNNNCTG 1 cut(s) 88
PvuII CAGCTG 1 cut(s) 88
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
SacI GAGCTC 2 cut(s) 82, 352
SatI GCNGC 3 cut(s) 86, 120, 213
SduI GDGCHC 2 cut(s) 82, 352
SfaNI GCATC 2 cut(s) 177, 236
SphI GCATGC 1 cut(s) 168
SsiI CCGC 1 cut(s) 301
SspMI CTAG 1 cut(s) 415
SstI GAGCTC 2 cut(s) 82, 352
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 1 cut(s) 400
TaiI ACGT 1 cut(s) 197
TaqI TCGA 1 cut(s) 5
TaqII GACCGA 1 cut(s) 260
TatI WGTACW 1 cut(s) 90
TscAI CASTG 2 cut(s) 36, 405
TseI GCWGC 3 cut(s) 85, 119, 212
TspDTI ATGAA 1 cut(s) 276
TspRI CASTG 2 cut(s) 36, 405
XceI RCATGY 1 cut(s) 168
XcmI CCANNNNNNNNNTGG 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 131
XspI CTAG 1 cut(s) 415
Zsp2I ATGCAT 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.