RLG00000031303

Golgi to ER traffic protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008175
Physical Location & Seq
Forward (+)
3882156 .. 3883395
1240 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031303

Sequence Viewer

Length: 564 bp
ATGTCGAAGGAGAGAGCCAAAACAGGCACACTGCCTCCAGCCCAAGAGCAAATTGAGAAATTTGAGAAAGTTGTAGAGGAAGGTAACTACTATGGAGCTCAGCAGCTGTACAAATCTATTAGTGCGAGATATGTAGCTACTCAGAGGTATACTGAAGCTTTAGATATTCTTCAGTCGGGTGCATGCATCCAGTTGAAACATGGGCAGGTTACGTGTGGGGCAGAGTTAGCTGCTTTGTTTGTGGATACACTTGTCAAAGCGAGATGCCCTTATGACGATGAAACCCTCGGTCAGTTGCTCGCCTCCAGCGTCAAGCACCGCCTCCCTCCAGTCCATCCTTCCCTCTTCTCTGTTGCCTCTGCCGGAGCTCCTTCAGTCTATTTCTTCCATCAAGGCCGATTACATTGTTCGCCAACAGGTCTTCTATCCCTTCACCTTTTCTACCTTTCGTTTTTACGCATACTCTCAATTTATTATTATCATCAATCACAACCAGGTTCAACACGCTCATGCTTGCCATTTATTCTAAATCTATGGGTATATAGAAAATCAGTAGTTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF410

Protein Analysis

188

Amino Acids

20.92

Weight (kDa)

8.94

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GET4 PF04190 48 - 100 3e-12 Golgi to ER traffic protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 149
AciI CCGC 1 cut(s) 319
AcsI RAATTY 1 cut(s) 59
AcuI CTGAAG 3 cut(s) 155, 174, 357
AfaI GTAC 1 cut(s) 110
AflIII ACRYGT 1 cut(s) 212
AgsI TTSAA 2 cut(s) 196, 501
AjnI CCWGG 1 cut(s) 493
AluBI AGCT 6 cut(s) 98, 106, 137, 158, 230, 368
AluI AGCT 6 cut(s) 98, 106, 137, 158, 230, 368
Alw21I GWGCWC 2 cut(s) 100, 370
AlwNI CAGNNNCTG 1 cut(s) 106
AoxI GGCC 1 cut(s) 394
ApeKI GCWGC 2 cut(s) 103, 230
ApoI RAATTY 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 425
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BanII GRGCYC 2 cut(s) 100, 370
BbsI GAAGAC 1 cut(s) 413
Bbv12I GWGCWC 2 cut(s) 100, 370
BbvI GCAGC 2 cut(s) 115, 217
BccI CCATC 2 cut(s) 342, 396
BciT130I CCWGG 1 cut(s) 495
BciVI GTATCC 1 cut(s) 238
BfuAI ACCTGC 1 cut(s) 196
BfuI GTATCC 1 cut(s) 238
BisI GCNGC 2 cut(s) 104, 231
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 2 cut(s) 105, 232
Bme1390I CCNGG 1 cut(s) 495
BmrFI CCNGG 1 cut(s) 495
BmsI GCATC 2 cut(s) 195, 254
BpiI GAAGAC 1 cut(s) 413
BpmI CTGGAG 3 cut(s) 21, 289, 312
Bpu1102I GCTNAGC 1 cut(s) 99
BsaAI YACGTR 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 286
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bse1I ACTGG 2 cut(s) 190, 329
BseBI CCWGG 1 cut(s) 495
BseDI CCNNGG 1 cut(s) 286
BseGI GGATG 2 cut(s) 186, 334
BseMII CTCAG 2 cut(s) 113, 155
BseNI ACTGG 2 cut(s) 190, 329
BseXI GCAGC 2 cut(s) 115, 217
BshFI GGCC 1 cut(s) 396
BsiHKAI GWGCWC 2 cut(s) 100, 370
BsiSI CCGG 1 cut(s) 363
BsnI GGCC 1 cut(s) 396
Bsp1286I GDGCHC 2 cut(s) 100, 370
Bsp1407I TGTACA 1 cut(s) 108
Bsp1720I GCTNAGC 1 cut(s) 99
BspACI CCGC 1 cut(s) 319
BspANI GGCC 1 cut(s) 396
BspCNI CTCAG 2 cut(s) 112, 154
BspMI ACCTGC 1 cut(s) 196
BsrGI TGTACA 1 cut(s) 108
BsrI ACTGG 2 cut(s) 190, 329
BssECI CCNNGG 1 cut(s) 286
BssNAI GTATAC 1 cut(s) 150
Bst1107I GTATAC 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 495
Bst6I CTCTTC 1 cut(s) 350
BstAUI TGTACA 1 cut(s) 108
BstBAI YACGTR 1 cut(s) 213
BstC8I GCNNGC 3 cut(s) 184, 300, 515
BstDEI CTNAG 2 cut(s) 99, 141
BstF5I GGATG 2 cut(s) 186, 334
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstNI CCWGG 1 cut(s) 495
BstNSI RCATGY 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 493
BstV1I GCAGC 2 cut(s) 115, 217
BstV2I GAAGAC 1 cut(s) 413
BstZ17I GTATAC 1 cut(s) 150
BsuI GTATCC 1 cut(s) 238
BsuRI GGCC 1 cut(s) 396
BtsCI GGATG 2 cut(s) 186, 334
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 29
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 3 cut(s) 184, 300, 515
CaiI CAGNNNCTG 1 cut(s) 106
CseI GACGC 1 cut(s) 298
CsiI ACCWGGT 1 cut(s) 493
Csp6I GTAC 1 cut(s) 109
CviAII CATG 3 cut(s) 183, 200, 510
CviJI RGCY 9 cut(s) 17, 41, 98, 106, 137, 158, 230, 368, 396
CviKI_1 RGCY 9 cut(s) 17, 41, 98, 106, 137, 158, 230, 368, 396
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 2 cut(s) 99, 141
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
Ecl136II GAGCTC 2 cut(s) 98, 368
Eco24I GRGCYC 2 cut(s) 100, 370
Eco53kI GAGCTC 2 cut(s) 98, 368
Eco57I CTGAAG 3 cut(s) 155, 174, 357
EcoICRI GAGCTC 2 cut(s) 98, 368
EcoRII CCWGG 1 cut(s) 493
EcoT22I ATGCAT 1 cut(s) 188
EcoT38I GRGCYC 2 cut(s) 100, 370
FaeI CATG 3 cut(s) 186, 203, 513
FatI CATG 3 cut(s) 182, 199, 509
FblI GTMKAC 1 cut(s) 149
Fnu4HI GCNGC 2 cut(s) 104, 231
FokI GGATG 2 cut(s) 173, 321
FriOI GRGCYC 2 cut(s) 100, 370
Fsp4HI GCNGC 2 cut(s) 104, 231
GluI GCNGC 2 cut(s) 104, 231
GsuI CTGGAG 3 cut(s) 21, 289, 312
HaeIII GGCC 1 cut(s) 396
HapII CCGG 1 cut(s) 363
HgaI GACGC 1 cut(s) 298
Hin1II CATG 3 cut(s) 186, 203, 513
HindIII AAGCTT 1 cut(s) 156
HpaII CCGG 1 cut(s) 363
HphI GGTGA 1 cut(s) 425
Hpy166II GTNNAC 1 cut(s) 150
Hpy188I TCNGA 1 cut(s) 144
Hpy188III TCNNGA 1 cut(s) 561
Hpy8I GTNNAC 1 cut(s) 150
HpyAV CCTTC 4 cut(s) 74, 348, 381, 440
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 2 cut(s) 182, 186
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 2 cut(s) 99, 141
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 3 cut(s) 186, 203, 513
LmnI GCTCC 3 cut(s) 95, 365, 373
Lsp1109I GCAGC 2 cut(s) 115, 217
LweI GCATC 2 cut(s) 195, 254
MabI ACCWGGT 1 cut(s) 493
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 2 cut(s) 83, 208
MboII GAAGA 4 cut(s) 161, 337, 376, 413
MhlI GDGCHC 2 cut(s) 100, 370
MluCI AATT 3 cut(s) 51, 59, 468
MnlI CCTC 9 cut(s) 45, 70, 138, 296, 313, 332, 336, 353, 367
Mph1103I ATGCAT 1 cut(s) 188
MslI CAYNNNNRTG 1 cut(s) 508
MspA1I CMGCKG 1 cut(s) 106
MspI CCGG 1 cut(s) 363
MspR9I CCNGG 1 cut(s) 495
MvaI CCWGG 1 cut(s) 495
MwoI GCNNNNNNNGC 1 cut(s) 227
NlaIII CATG 3 cut(s) 186, 203, 513
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 1 cut(s) 186
PaeI GCATGC 1 cut(s) 186
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PkrI GCNGC 2 cut(s) 105, 232
Ppu21I YACGTR 1 cut(s) 213
Psp124BI GAGCTC 2 cut(s) 100, 370
Psp6I CCWGG 1 cut(s) 493
PspGI CCWGG 1 cut(s) 493
PstNI CAGNNNCTG 1 cut(s) 106
PvuII CAGCTG 1 cut(s) 106
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
RseI CAYNNNNRTG 1 cut(s) 508
SacI GAGCTC 2 cut(s) 100, 370
SatI GCNGC 2 cut(s) 104, 231
ScrFI CCNGG 1 cut(s) 495
SduI GDGCHC 2 cut(s) 100, 370
SexAI ACCWGGT 1 cut(s) 493
SfaNI GCATC 2 cut(s) 195, 254
SmiMI CAYNNNNRTG 1 cut(s) 508
SphI GCATGC 1 cut(s) 186
Sse9I AATT 3 cut(s) 51, 59, 468
SsiI CCGC 1 cut(s) 319
SstI GAGCTC 2 cut(s) 100, 370
StyD4I CCNGG 1 cut(s) 493
TaiI ACGT 1 cut(s) 215
TaqI TCGA 1 cut(s) 5
TaqII GACCGA 1 cut(s) 278
TasI AATT 3 cut(s) 51, 59, 468
TatI WGTACW 1 cut(s) 108
TscAI CASTG 1 cut(s) 36
TseI GCWGC 2 cut(s) 103, 230
TspDTI ATGAA 1 cut(s) 294
TspRI CASTG 1 cut(s) 36
XapI RAATTY 1 cut(s) 59
XceI RCATGY 1 cut(s) 186
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 149
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.