Rh2BG092200

Golgi to ER traffic protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008175
Physical Location & Seq
Forward (+)
7174952 .. 7177815
2864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG092200.1

Sequence Viewer

Length: 450 bp
ATGTCGAAGGAGAGAGCCAAAAGAGGCACATTGCCTCCAGCCCAAGAGCAAATTGAGAAATTTGAGAAAGTTGTAGAGGAAGGTAACTACTATGGAGCTCAGCAGCTGTACAAATCTATTAGTGCGAGATATGTAGCTGCTCAGAGGTATACTGAAGCTTTAGATATTCTTCAGTCGGGTGCATGCATCCAGTTGAAACATGGGCAGGTTACGTGTGGGGCAGAGTTAGCTGCTTTGTTTGTGGATACACTTGTCAAAGCGAGATGCCCTTATGACGATGAAACCCTCGGTCAGTTGCTCGCCTCCAGATTGCTCGCCTCCAGCGTCAAGCACCGCCTCCCTCCGGTCCATCCTTCCCTCTTCTCTGTTGCCTCTGCCGGAGCTCCTTCAGTCTATTTCTTCCATCAAGGCCGATTACATTGCTCGCCAACAGTGCAAAGCATCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF410

Protein Analysis

149

Amino Acids

16.36

Weight (kDa)

8.69

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GET4 PF04190 48 - 100 1.1e-12 Golgi to ER traffic protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 149
AciI CCGC 1 cut(s) 334
AcsI RAATTY 1 cut(s) 59
AcuI CTGAAG 3 cut(s) 155, 174, 372
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 1 cut(s) 343
AflIII ACRYGT 1 cut(s) 212
AgsI TTSAA 1 cut(s) 196
AluBI AGCT 6 cut(s) 98, 106, 137, 158, 230, 383
AluI AGCT 6 cut(s) 98, 106, 137, 158, 230, 383
Alw21I GWGCWC 2 cut(s) 100, 385
AlwNI CAGNNNCTG 1 cut(s) 106
AoxI GGCC 1 cut(s) 409
ApeKI GCWGC 3 cut(s) 103, 137, 230
ApoI RAATTY 1 cut(s) 59
AspS9I GGNCC 1 cut(s) 346
AvaII GGWCC 1 cut(s) 346
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BanII GRGCYC 2 cut(s) 100, 385
Bbv12I GWGCWC 2 cut(s) 100, 385
BbvI GCAGC 3 cut(s) 115, 124, 217
BccI CCATC 2 cut(s) 357, 411
BcgI CGANNNNNNTGC 2 cut(s) 402, 436
BciVI GTATCC 1 cut(s) 238
BfaI CTAG 1 cut(s) 448
BfuAI ACCTGC 1 cut(s) 196
BfuI GTATCC 1 cut(s) 238
BisI GCNGC 3 cut(s) 104, 138, 231
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 3 cut(s) 105, 139, 232
Bme18I GGWCC 1 cut(s) 346
BmgT120I GGNCC 1 cut(s) 346
BmsI GCATC 2 cut(s) 195, 254
BpmI CTGGAG 3 cut(s) 21, 289, 304
Bpu1102I GCTNAGC 1 cut(s) 99
BsaAI YACGTR 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 286
BsaWI WCCGGW 1 cut(s) 343
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 1 cut(s) 343
Bse1I ACTGG 1 cut(s) 190
Bse3DI GCAATG 2 cut(s) 29, 418
BseDI CCNNGG 1 cut(s) 286
BseGI GGATG 3 cut(s) 186, 349, 441
BseLI CCNNNNNNNGG 1 cut(s) 343
BseMI GCAATG 2 cut(s) 29, 418
BseMII CTCAG 2 cut(s) 113, 155
BseNI ACTGG 1 cut(s) 190
BseXI GCAGC 3 cut(s) 115, 124, 217
BshFI GGCC 1 cut(s) 411
BsiHKAI GWGCWC 2 cut(s) 100, 385
BsiSI CCGG 2 cut(s) 344, 378
BslI CCNNNNNNNGG 1 cut(s) 343
BsnI GGCC 1 cut(s) 411
Bsp1286I GDGCHC 2 cut(s) 100, 385
Bsp1407I TGTACA 1 cut(s) 108
Bsp1720I GCTNAGC 1 cut(s) 99
BspACI CCGC 1 cut(s) 334
BspANI GGCC 1 cut(s) 411
BspCNI CTCAG 2 cut(s) 112, 154
BspMI ACCTGC 1 cut(s) 196
BsrDI GCAATG 2 cut(s) 29, 418
BsrGI TGTACA 1 cut(s) 108
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 1 cut(s) 286
BssNAI GTATAC 1 cut(s) 150
Bst1107I GTATAC 1 cut(s) 150
Bst4CI ACNGT 1 cut(s) 433
Bst6I CTCTTC 1 cut(s) 365
BstAUI TGTACA 1 cut(s) 108
BstBAI YACGTR 1 cut(s) 213
BstC8I GCNNGC 4 cut(s) 184, 300, 315, 425
BstDEI CTNAG 2 cut(s) 99, 141
BstF5I GGATG 3 cut(s) 186, 349, 441
BstMWI GCNNNNNNNGC 2 cut(s) 227, 433
BstNSI RCATGY 1 cut(s) 186
BstV1I GCAGC 3 cut(s) 115, 124, 217
BstZ17I GTATAC 1 cut(s) 150
BsuI GTATCC 1 cut(s) 238
BsuRI GGCC 1 cut(s) 411
BtsCI GGATG 3 cut(s) 186, 349, 441
BtsIMutI CAGTG 1 cut(s) 438
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 4 cut(s) 184, 300, 315, 425
CaiI CAGNNNCTG 1 cut(s) 106
Cfr13I GGNCC 1 cut(s) 346
CseI GACGC 1 cut(s) 313
Csp6I GTAC 1 cut(s) 109
CviAII CATG 2 cut(s) 183, 200
CviJI RGCY 9 cut(s) 17, 41, 98, 106, 137, 158, 230, 383, 411
CviKI_1 RGCY 9 cut(s) 17, 41, 98, 106, 137, 158, 230, 383, 411
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 2 cut(s) 99, 141
Eam1104I CTCTTC 1 cut(s) 365
EarI CTCTTC 1 cut(s) 365
Ecl136II GAGCTC 2 cut(s) 98, 383
Eco24I GRGCYC 2 cut(s) 100, 385
Eco47I GGWCC 1 cut(s) 346
Eco53kI GAGCTC 2 cut(s) 98, 383
Eco57I CTGAAG 3 cut(s) 155, 174, 372
EcoICRI GAGCTC 2 cut(s) 98, 383
EcoT22I ATGCAT 1 cut(s) 188
EcoT38I GRGCYC 2 cut(s) 100, 385
FaeI CATG 2 cut(s) 186, 203
FaiI YATR 6 cut(s) 93, 132, 150, 184, 201, 273
FatI CATG 2 cut(s) 182, 199
FblI GTMKAC 1 cut(s) 149
Fnu4HI GCNGC 3 cut(s) 104, 138, 231
FokI GGATG 3 cut(s) 173, 336, 428
FriOI GRGCYC 2 cut(s) 100, 385
Fsp4HI GCNGC 3 cut(s) 104, 138, 231
FspBI CTAG 1 cut(s) 448
GluI GCNGC 3 cut(s) 104, 138, 231
GsuI CTGGAG 3 cut(s) 21, 289, 304
HaeIII GGCC 1 cut(s) 411
HapII CCGG 2 cut(s) 344, 378
HgaI GACGC 1 cut(s) 313
Hin1II CATG 2 cut(s) 186, 203
HindIII AAGCTT 1 cut(s) 156
HpaII CCGG 2 cut(s) 344, 378
Hpy166II GTNNAC 1 cut(s) 150
Hpy188I TCNGA 1 cut(s) 144
Hpy188III TCNNGA 1 cut(s) 306
Hpy8I GTNNAC 1 cut(s) 150
HpyAV CCTTC 3 cut(s) 74, 363, 396
HpyCH4III ACNGT 1 cut(s) 433
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 3 cut(s) 182, 186, 436
HpyF10VI GCNNNNNNNGC 2 cut(s) 227, 433
HpyF3I CTNAG 2 cut(s) 99, 141
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 2 cut(s) 186, 203
LmnI GCTCC 3 cut(s) 95, 380, 388
LpnPI CCDG 7 cut(s) 51, 191, 203, 319, 334, 357, 391
Lsp1109I GCAGC 3 cut(s) 115, 124, 217
LweI GCATC 2 cut(s) 195, 254
MaeI CTAG 1 cut(s) 448
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 2 cut(s) 83, 208
MboII GAAGA 3 cut(s) 161, 352, 391
MhlI GDGCHC 2 cut(s) 100, 385
MluCI AATT 2 cut(s) 51, 59
Mph1103I ATGCAT 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 106
MspI CCGG 2 cut(s) 344, 378
MwoI GCNNNNNNNGC 2 cut(s) 227, 433
NlaIII CATG 2 cut(s) 186, 203
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 1 cut(s) 186
PaeI GCATGC 1 cut(s) 186
PcsI WCGNNNNNNNCGW 1 cut(s) 321
PkrI GCNGC 3 cut(s) 105, 139, 232
Ppu21I YACGTR 1 cut(s) 213
Psp124BI GAGCTC 2 cut(s) 100, 385
PspPI GGNCC 1 cut(s) 346
PstNI CAGNNNCTG 1 cut(s) 106
PvuII CAGCTG 1 cut(s) 106
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SacI GAGCTC 2 cut(s) 100, 385
SatI GCNGC 3 cut(s) 104, 138, 231
Sau96I GGNCC 1 cut(s) 346
SduI GDGCHC 2 cut(s) 100, 385
SfaNI GCATC 2 cut(s) 195, 254
SinI GGWCC 1 cut(s) 346
SphI GCATGC 1 cut(s) 186
Sse9I AATT 2 cut(s) 51, 59
SsiI CCGC 1 cut(s) 334
SspMI CTAG 1 cut(s) 448
SstI GAGCTC 2 cut(s) 100, 385
TaaI ACNGT 1 cut(s) 433
TaiI ACGT 1 cut(s) 215
TaqI TCGA 1 cut(s) 5
TaqII GACCGA 1 cut(s) 278
TasI AATT 2 cut(s) 51, 59
TatI WGTACW 1 cut(s) 108
TscAI CASTG 1 cut(s) 438
TseI GCWGC 3 cut(s) 103, 137, 230
TspDTI ATGAA 1 cut(s) 294
TspRI CASTG 1 cut(s) 438
VpaK11BI GGWCC 1 cut(s) 346
XapI RAATTY 1 cut(s) 59
XceI RCATGY 1 cut(s) 186
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 149
XspI CTAG 1 cut(s) 448
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.