RchiOBHm_Chr5g0038701

Golgi to ER traffic protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008175
Physical Location & Seq
Forward (+)
33453898 .. 33456778
2881 bp
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UTR
Exon/CDS
Intron
PRQ31730

Sequence Viewer

Length: 435 bp
ATGTCGAAGGAGAGAGCCAAAAGAGGCACACTGCCTCCAGCCCAAGAGCAAATTGAGAAATTTGAGAAAGTTGTAGAGGAAGGTAACTACCATGGAGCTCAGCAGCTGTACAAATCTATTAGTGCGAGATATGTAGCTGCTCAGAGGTATACTGAAGCTTTAGATATTCTTCAGTCGGGTGCATGCATCCAGTTGAAACATGGGCAGGTTACGTGTGGGGCAGAGTTAGCTGCTTTGTTTGTGGATACACTTGTCAAAGCGAGATGCCCTTATGACGATGAAACCCTCGGTCAGTTGCTCGCCTCCAGCGTCAAGCACCGCCTCCCTCCAGTCCATCCTTCCCTCTTCTCTGTTGCCTCTGCCGGAGCTCCTTCAGTCTATTTCTTCCATCAAGGCCGATTACATTGCTCGCCAACAGTGCAAAGCATCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF410

Protein Analysis

144

Amino Acids

15.8

Weight (kDa)

8.36

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GET4 PF04190 48 - 100 1.2e-12 Golgi to ER traffic protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 149
AciI CCGC 1 cut(s) 319
AcsI RAATTY 1 cut(s) 59
AcuI CTGAAG 3 cut(s) 155, 174, 357
AfaI GTAC 1 cut(s) 110
AflIII ACRYGT 1 cut(s) 212
AgsI TTSAA 1 cut(s) 196
AluBI AGCT 6 cut(s) 98, 106, 137, 158, 230, 368
AluI AGCT 6 cut(s) 98, 106, 137, 158, 230, 368
Alw21I GWGCWC 2 cut(s) 100, 370
AlwNI CAGNNNCTG 1 cut(s) 106
AoxI GGCC 1 cut(s) 394
ApeKI GCWGC 3 cut(s) 103, 137, 230
ApoI RAATTY 1 cut(s) 59
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BanII GRGCYC 2 cut(s) 100, 370
Bbv12I GWGCWC 2 cut(s) 100, 370
BbvI GCAGC 3 cut(s) 115, 124, 217
BccI CCATC 2 cut(s) 342, 396
BcgI CGANNNNNNTGC 2 cut(s) 387, 421
BciVI GTATCC 1 cut(s) 238
BfaI CTAG 1 cut(s) 433
BfuAI ACCTGC 1 cut(s) 196
BfuI GTATCC 1 cut(s) 238
BisI GCNGC 3 cut(s) 104, 138, 231
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 3 cut(s) 105, 139, 232
BmsI GCATC 2 cut(s) 195, 254
BpmI CTGGAG 3 cut(s) 21, 289, 312
Bpu1102I GCTNAGC 1 cut(s) 99
BsaAI YACGTR 1 cut(s) 213
BsaJI CCNNGG 2 cut(s) 91, 286
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bse1I ACTGG 2 cut(s) 190, 329
Bse3DI GCAATG 1 cut(s) 403
BseDI CCNNGG 2 cut(s) 91, 286
BseGI GGATG 3 cut(s) 186, 334, 426
BseMI GCAATG 1 cut(s) 403
BseMII CTCAG 2 cut(s) 113, 155
BseNI ACTGG 2 cut(s) 190, 329
BseXI GCAGC 3 cut(s) 115, 124, 217
BshFI GGCC 1 cut(s) 396
BsiHKAI GWGCWC 2 cut(s) 100, 370
BsiSI CCGG 1 cut(s) 363
BsnI GGCC 1 cut(s) 396
Bsp1286I GDGCHC 2 cut(s) 100, 370
Bsp1407I TGTACA 1 cut(s) 108
Bsp1720I GCTNAGC 1 cut(s) 99
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 1 cut(s) 319
BspANI GGCC 1 cut(s) 396
BspCNI CTCAG 2 cut(s) 112, 154
BspMI ACCTGC 1 cut(s) 196
BsrDI GCAATG 1 cut(s) 403
BsrGI TGTACA 1 cut(s) 108
BsrI ACTGG 2 cut(s) 190, 329
BssECI CCNNGG 2 cut(s) 91, 286
BssNAI GTATAC 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 91
Bst1107I GTATAC 1 cut(s) 150
Bst4CI ACNGT 1 cut(s) 418
Bst6I CTCTTC 1 cut(s) 350
BstAUI TGTACA 1 cut(s) 108
BstBAI YACGTR 1 cut(s) 213
BstC8I GCNNGC 3 cut(s) 184, 300, 410
BstDEI CTNAG 2 cut(s) 99, 141
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 3 cut(s) 186, 334, 426
BstMWI GCNNNNNNNGC 2 cut(s) 227, 418
BstNSI RCATGY 1 cut(s) 186
BstV1I GCAGC 3 cut(s) 115, 124, 217
BstZ17I GTATAC 1 cut(s) 150
BsuI GTATCC 1 cut(s) 238
BsuRI GGCC 1 cut(s) 396
BtgI CCRYGG 1 cut(s) 91
BtsCI GGATG 3 cut(s) 186, 334, 426
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 2 cut(s) 29, 423
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 3 cut(s) 184, 300, 410
CaiI CAGNNNCTG 1 cut(s) 106
CseI GACGC 1 cut(s) 298
Csp6I GTAC 1 cut(s) 109
CviAII CATG 3 cut(s) 92, 183, 200
CviJI RGCY 9 cut(s) 17, 41, 98, 106, 137, 158, 230, 368, 396
CviKI_1 RGCY 9 cut(s) 17, 41, 98, 106, 137, 158, 230, 368, 396
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 2 cut(s) 99, 141
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
Ecl136II GAGCTC 2 cut(s) 98, 368
Eco130I CCWWGG 1 cut(s) 91
Eco24I GRGCYC 2 cut(s) 100, 370
Eco53kI GAGCTC 2 cut(s) 98, 368
Eco57I CTGAAG 3 cut(s) 155, 174, 357
EcoICRI GAGCTC 2 cut(s) 98, 368
EcoT14I CCWWGG 1 cut(s) 91
EcoT22I ATGCAT 1 cut(s) 188
EcoT38I GRGCYC 2 cut(s) 100, 370
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 3 cut(s) 95, 186, 203
FaiI YATR 6 cut(s) 93, 132, 150, 184, 201, 273
FatI CATG 3 cut(s) 91, 182, 199
FblI GTMKAC 1 cut(s) 149
Fnu4HI GCNGC 3 cut(s) 104, 138, 231
FokI GGATG 3 cut(s) 173, 321, 413
FriOI GRGCYC 2 cut(s) 100, 370
Fsp4HI GCNGC 3 cut(s) 104, 138, 231
FspBI CTAG 1 cut(s) 433
GluI GCNGC 3 cut(s) 104, 138, 231
GsuI CTGGAG 3 cut(s) 21, 289, 312
HaeIII GGCC 1 cut(s) 396
HapII CCGG 1 cut(s) 363
HgaI GACGC 1 cut(s) 298
Hin1II CATG 3 cut(s) 95, 186, 203
HindIII AAGCTT 1 cut(s) 156
HpaII CCGG 1 cut(s) 363
Hpy166II GTNNAC 1 cut(s) 150
Hpy188I TCNGA 1 cut(s) 144
Hpy8I GTNNAC 1 cut(s) 150
HpyAV CCTTC 3 cut(s) 74, 348, 381
HpyCH4III ACNGT 1 cut(s) 418
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 3 cut(s) 182, 186, 421
HpyF10VI GCNNNNNNNGC 2 cut(s) 227, 418
HpyF3I CTNAG 2 cut(s) 99, 141
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 3 cut(s) 95, 186, 203
LmnI GCTCC 3 cut(s) 95, 365, 373
LpnPI CCDG 6 cut(s) 51, 191, 203, 319, 342, 376
Lsp1109I GCAGC 3 cut(s) 115, 124, 217
LweI GCATC 2 cut(s) 195, 254
MaeI CTAG 1 cut(s) 433
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 2 cut(s) 83, 208
MboII GAAGA 3 cut(s) 161, 337, 376
MhlI GDGCHC 2 cut(s) 100, 370
MluCI AATT 2 cut(s) 51, 59
Mph1103I ATGCAT 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 106
MspI CCGG 1 cut(s) 363
MwoI GCNNNNNNNGC 2 cut(s) 227, 418
NcoI CCATGG 1 cut(s) 91
NlaIII CATG 3 cut(s) 95, 186, 203
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 1 cut(s) 186
PaeI GCATGC 1 cut(s) 186
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PkrI GCNGC 3 cut(s) 105, 139, 232
Ppu21I YACGTR 1 cut(s) 213
Psp124BI GAGCTC 2 cut(s) 100, 370
PstNI CAGNNNCTG 1 cut(s) 106
PvuII CAGCTG 1 cut(s) 106
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SacI GAGCTC 2 cut(s) 100, 370
SatI GCNGC 3 cut(s) 104, 138, 231
SduI GDGCHC 2 cut(s) 100, 370
SfaNI GCATC 2 cut(s) 195, 254
SphI GCATGC 1 cut(s) 186
Sse9I AATT 2 cut(s) 51, 59
SsiI CCGC 1 cut(s) 319
SspMI CTAG 1 cut(s) 433
SstI GAGCTC 2 cut(s) 100, 370
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 1 cut(s) 418
TaiI ACGT 1 cut(s) 215
TaqI TCGA 1 cut(s) 5
TaqII GACCGA 1 cut(s) 278
TasI AATT 2 cut(s) 51, 59
TatI WGTACW 1 cut(s) 108
TscAI CASTG 2 cut(s) 36, 423
TseI GCWGC 3 cut(s) 103, 137, 230
TspDTI ATGAA 1 cut(s) 294
TspRI CASTG 2 cut(s) 36, 423
XapI RAATTY 1 cut(s) 59
XceI RCATGY 1 cut(s) 186
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 149
XspI CTAG 1 cut(s) 433
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.