Rh3BG051800

Golgi to ER traffic protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0008175
Physical Location & Seq
Reverse (-)
3515586 .. 3520900
5315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG051800.1

Sequence Viewer

Length: 480 bp
ATGTCGAAGGAGAGAGCCAAAAGAGGCACACTGCCTCCAGCCCAAGAGCAAATTGAAAAATTTGAGAAAGTTGTAAAGGAAGGTAACTACTATGCAGCTCAGCAGCTGTACAAATCTATTAGTGCGAGATATGTAGCTGCTCAGAGGTATACTGAAGATTTAGATATTCTTCAGTCGGGTGCATGCATCCAGTTGAAACATGGGCAGGTTACGTGTGGGGCAGAGTTAGCTGCTTTGTTTGTGGATACACTTGTCAAAGCGAGATGCCCTTATGACGATGAAACCCTCGAGCTCCTTCAGTCTATTTCTTCCATCAAGGCCGATTACATTGCTCGCCAACAGTGCAAAGCATCCTCTAGTCCTCTAGTCCTCTGCACTGATGATTCTGGGGTCTTTTCTACCAGCCCTTCCAACGAGTACAACCTTGCTGCTTCTGCATTTGGTATGTCCGATGTCACTAGTGCATTTAATATAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF410

Protein Analysis

159

Amino Acids

17.42

Weight (kDa)

5.45

Isoelectric Point (pI)

55.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GET4 PF04190 48 - 108 4.8e-11 Golgi to ER traffic protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 149
AcsI RAATTY 1 cut(s) 59
AcuI CTGAAG 3 cut(s) 155, 174, 281
AfaI GTAC 2 cut(s) 110, 419
AflIII ACRYGT 1 cut(s) 212
AgsI TTSAA 2 cut(s) 56, 196
AhlI ACTAGT 1 cut(s) 458
AluBI AGCT 5 cut(s) 98, 106, 137, 230, 292
AluI AGCT 5 cut(s) 98, 106, 137, 230, 292
Alw21I GWGCWC 1 cut(s) 294
AlwNI CAGNNNCTG 1 cut(s) 106
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 1 cut(s) 318
ApeKI GCWGC 5 cut(s) 95, 103, 137, 230, 428
ApoI RAATTY 1 cut(s) 59
AvaI CYCGRG 1 cut(s) 287
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BanII GRGCYC 1 cut(s) 294
Bbv12I GWGCWC 1 cut(s) 294
BbvI GCAGC 5 cut(s) 107, 115, 124, 217, 415
BccI CCATC 1 cut(s) 320
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciVI GTATCC 1 cut(s) 238
BcuI ACTAGT 1 cut(s) 458
BfaI CTAG 3 cut(s) 357, 365, 459
BfuAI ACCTGC 1 cut(s) 196
BfuI GTATCC 1 cut(s) 238
BisI GCNGC 5 cut(s) 96, 104, 138, 231, 429
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 5 cut(s) 97, 105, 139, 232, 430
BmeT110I CYCGRG 1 cut(s) 287
BmsI GCATC 3 cut(s) 195, 254, 359
BpmI CTGGAG 1 cut(s) 21
Bpu1102I GCTNAGC 1 cut(s) 99
BsaAI YACGTR 1 cut(s) 213
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bse1I ACTGG 1 cut(s) 190
Bse3DI GCAATG 1 cut(s) 327
BseGI GGATG 2 cut(s) 186, 350
BseMI GCAATG 1 cut(s) 327
BseMII CTCAG 2 cut(s) 113, 155
BseNI ACTGG 1 cut(s) 190
BseXI GCAGC 5 cut(s) 107, 115, 124, 217, 415
BsgI GTGCAG 1 cut(s) 358
BshFI GGCC 1 cut(s) 320
BsiHKAI GWGCWC 1 cut(s) 294
BsiHKCI CYCGRG 1 cut(s) 287
BsnI GGCC 1 cut(s) 320
BsoBI CYCGRG 1 cut(s) 287
Bsp1286I GDGCHC 1 cut(s) 294
Bsp1407I TGTACA 1 cut(s) 108
Bsp1720I GCTNAGC 1 cut(s) 99
BspANI GGCC 1 cut(s) 320
BspCNI CTCAG 2 cut(s) 112, 154
BspMI ACCTGC 1 cut(s) 196
BsrDI GCAATG 1 cut(s) 327
BsrGI TGTACA 1 cut(s) 108
BsrI ACTGG 1 cut(s) 190
BssNAI GTATAC 1 cut(s) 150
Bst1107I GTATAC 1 cut(s) 150
Bst4CI ACNGT 1 cut(s) 342
BstAUI TGTACA 1 cut(s) 108
BstBAI YACGTR 1 cut(s) 213
BstC8I GCNNGC 2 cut(s) 184, 334
BstDEI CTNAG 2 cut(s) 99, 141
BstF5I GGATG 2 cut(s) 186, 350
BstMWI GCNNNNNNNGC 3 cut(s) 227, 342, 434
BstNSI RCATGY 1 cut(s) 186
BstV1I GCAGC 5 cut(s) 107, 115, 124, 217, 415
BstZ17I GTATAC 1 cut(s) 150
BsuI GTATCC 1 cut(s) 238
BsuRI GGCC 1 cut(s) 320
BtsCI GGATG 2 cut(s) 186, 350
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 3 cut(s) 29, 347, 375
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 2 cut(s) 184, 334
CaiI CAGNNNCTG 1 cut(s) 106
Csp6I GTAC 2 cut(s) 109, 418
CviAII CATG 2 cut(s) 183, 200
CviJI RGCY 9 cut(s) 17, 41, 98, 106, 137, 230, 292, 320, 405
CviKI_1 RGCY 9 cut(s) 17, 41, 98, 106, 137, 230, 292, 320, 405
CviQI GTAC 2 cut(s) 109, 418
DdeI CTNAG 2 cut(s) 99, 141
Ecl136II GAGCTC 1 cut(s) 292
Eco24I GRGCYC 1 cut(s) 294
Eco53kI GAGCTC 1 cut(s) 292
Eco57I CTGAAG 3 cut(s) 155, 174, 281
Eco88I CYCGRG 1 cut(s) 287
EcoICRI GAGCTC 1 cut(s) 292
EcoT22I ATGCAT 1 cut(s) 188
EcoT38I GRGCYC 1 cut(s) 294
FaeI CATG 2 cut(s) 186, 203
FaiI YATR 8 cut(s) 93, 132, 150, 184, 201, 273, 446, 473
FatI CATG 2 cut(s) 182, 199
FblI GTMKAC 1 cut(s) 149
Fnu4HI GCNGC 5 cut(s) 96, 104, 138, 231, 429
FokI GGATG 2 cut(s) 173, 337
FriOI GRGCYC 1 cut(s) 294
Fsp4HI GCNGC 5 cut(s) 96, 104, 138, 231, 429
FspBI CTAG 3 cut(s) 357, 365, 459
GluI GCNGC 5 cut(s) 96, 104, 138, 231, 429
GsuI CTGGAG 1 cut(s) 21
HaeIII GGCC 1 cut(s) 320
Hin1II CATG 2 cut(s) 186, 203
HinfI GANTC 1 cut(s) 383
Hpy166II GTNNAC 1 cut(s) 150
Hpy188I TCNGA 2 cut(s) 144, 451
Hpy8I GTNNAC 1 cut(s) 150
HpyAV CCTTC 3 cut(s) 74, 305, 417
HpyCH4III ACNGT 1 cut(s) 342
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 7 cut(s) 95, 182, 186, 345, 375, 437, 464
HpyF10VI GCNNNNNNNGC 3 cut(s) 227, 342, 434
HpyF3I CTNAG 2 cut(s) 99, 141
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 2 cut(s) 186, 203
LmnI GCTCC 1 cut(s) 297
LpnPI CCDG 5 cut(s) 51, 191, 203, 372, 415
Lsp1109I GCAGC 5 cut(s) 107, 115, 124, 217, 415
LweI GCATC 3 cut(s) 195, 254, 359
MaeI CTAG 3 cut(s) 357, 365, 459
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 3 cut(s) 83, 208, 454
MboII GAAGA 3 cut(s) 161, 167, 300
MhlI GDGCHC 1 cut(s) 294
MluCI AATT 2 cut(s) 51, 59
MmeI TCCRAC 1 cut(s) 435
MnlI CCTC 7 cut(s) 17, 45, 138, 296, 364, 372, 380
Mph1103I ATGCAT 1 cut(s) 188
MseI TTAA 1 cut(s) 468
MspA1I CMGCKG 1 cut(s) 106
MwoI GCNNNNNNNGC 3 cut(s) 227, 342, 434
NlaIII CATG 2 cut(s) 186, 203
NmuCI GTSAC 1 cut(s) 454
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 1 cut(s) 186
PaeI GCATGC 1 cut(s) 186
PaeR7I CTCGAG 1 cut(s) 287
PfeI GAWTC 1 cut(s) 383
PkrI GCNGC 5 cut(s) 97, 105, 139, 232, 430
Ppu21I YACGTR 1 cut(s) 213
Psp124BI GAGCTC 1 cut(s) 294
PspXI VCTCGAGB 1 cut(s) 287
PstNI CAGNNNCTG 1 cut(s) 106
PvuII CAGCTG 1 cut(s) 106
RsaI GTAC 2 cut(s) 110, 419
RsaNI GTAC 2 cut(s) 109, 418
SacI GAGCTC 1 cut(s) 294
SaqAI TTAA 1 cut(s) 468
SatI GCNGC 5 cut(s) 96, 104, 138, 231, 429
SduI GDGCHC 1 cut(s) 294
SfaNI GCATC 3 cut(s) 195, 254, 359
Sfr274I CTCGAG 1 cut(s) 287
SlaI CTCGAG 1 cut(s) 287
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SpeI ACTAGT 1 cut(s) 458
SphI GCATGC 1 cut(s) 186
Sse9I AATT 2 cut(s) 51, 59
SspMI CTAG 3 cut(s) 357, 365, 459
SstI GAGCTC 1 cut(s) 294
TaaI ACNGT 1 cut(s) 342
TaiI ACGT 1 cut(s) 215
TaqI TCGA 2 cut(s) 5, 288
TasI AATT 2 cut(s) 51, 59
TatI WGTACW 2 cut(s) 108, 417
TfiI GAWTC 1 cut(s) 383
Tru1I TTAA 1 cut(s) 468
Tru9I TTAA 1 cut(s) 468
TscAI CASTG 3 cut(s) 36, 347, 382
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 5 cut(s) 95, 103, 137, 230, 428
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 1 cut(s) 294
TspRI CASTG 3 cut(s) 36, 347, 382
XapI RAATTY 1 cut(s) 59
XceI RCATGY 1 cut(s) 186
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XhoI CTCGAG 1 cut(s) 287
XmiI GTMKAC 1 cut(s) 149
XspI CTAG 3 cut(s) 357, 365, 459
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.