RLG00000028845

Golgi to ER traffic protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008175
Physical Location & Seq
Reverse (-)
29160580 .. 29164726
4147 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028845

Sequence Viewer

Length: 783 bp
ATGTCGAAGGAGAGAGCCAAAACAGGCACACTGCCTCCAGCCCAAGAGCAAATTGAGAAATTTGAGAAAGTTGTAGAGGAAGGTAACTACTATGGAGCTCAGCAGCTGTACAAATCTATTAGTGCGAGATATGTAGCTACTCAGAGGTATACTGAAGCTTTAGATATTCTTCAGTCGGGTGCATGCATCCAGTTGAAACATGGGCAGGTTACGTGTGGGGCAGAGTTAGCTGCTTTGTTTGTGGATACACTTGTCAAAGCGAGATGCCCTTATGACGATGAAACCCTCGGTCAGTTGCTCGCCTCCAGCGCCGATTACATTGTTCGCCAACAGTGCAAAGCATCCTCTAGTCCTCTAGTCCTCTGCACCGATGATTCTGGGGTCTTTTCTACCAGCCCTTCCAACGAGTACAACCTTGCTGCTTCTGCATTTGTGCCACAACAATCGCCGCTTGCCACAACAAAGCTCCGTTCAAGCTCTTCACACGCCTTCCACCATCAAACCCAAAACCCATCTCTTGATGCAACCTCTCTCTCCCATCCCGACCCACATTCCTCATTGCCAAGCCCAAACCACTCTACACCTCCATCAAGGCCTTCGCTTCCGCCATTATCGCCAACCTTGACCTCGACTTTGACTTTCTCAGATGCCCAATCGACGGCCTCAGCGACTTCATCTCCGTCACCGTGGACCCACAAGTCTTCCCCGAAAAGATCTCCAAAACTCCTCACAATCGATATATGCGGGGCTCAAATCGAAGGAAAAGATCTCCCACCACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF410

Protein Analysis

261

Amino Acids

27.93

Weight (kDa)

6.7

Isoelectric Point (pI)

66.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GET4 PF04190 48 - 100 5.7e-12 Golgi to ER traffic protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 697
Acc36I ACCTGC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 149
AciI CCGC 3 cut(s) 449, 605, 744
AcsI RAATTY 1 cut(s) 59
AcuI CTGAAG 2 cut(s) 155, 174
AfaI GTAC 2 cut(s) 110, 410
AfiI CCNNNNNNNGG 1 cut(s) 658
AflIII ACRYGT 1 cut(s) 212
AgsI TTSAA 2 cut(s) 196, 474
AleI CACNNNNGTG 1 cut(s) 778
AluBI AGCT 7 cut(s) 98, 106, 137, 158, 230, 466, 477
AluI AGCT 7 cut(s) 98, 106, 137, 158, 230, 466, 477
Alw21I GWGCWC 1 cut(s) 100
AlwNI CAGNNNCTG 1 cut(s) 106
AoxI GGCC 2 cut(s) 593, 660
ApeKI GCWGC 3 cut(s) 103, 230, 419
ApoI RAATTY 1 cut(s) 59
ArsI GACNNNNNNTTYG 2 cut(s) 616, 648
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 1 cut(s) 690
AsuHPI GGTGA 1 cut(s) 675
AvaII GGWCC 1 cut(s) 690
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BanII GRGCYC 2 cut(s) 100, 751
BbsI GAAGAC 1 cut(s) 693
Bbv12I GWGCWC 1 cut(s) 100
BbvCI CCTCAGC 1 cut(s) 664
BbvI GCAGC 3 cut(s) 115, 217, 406
BccI CCATC 4 cut(s) 504, 520, 546, 595
BceAI ACGGC 1 cut(s) 675
BciVI GTATCC 1 cut(s) 238
BfaI CTAG 2 cut(s) 348, 356
BfoI RGCGCY 1 cut(s) 312
BfuAI ACCTGC 1 cut(s) 196
BfuI GTATCC 1 cut(s) 238
BglII AGATCT 2 cut(s) 713, 766
BisI GCNGC 4 cut(s) 104, 231, 420, 449
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 4 cut(s) 105, 232, 421, 450
Bme18I GGWCC 1 cut(s) 690
BmgT120I GGNCC 1 cut(s) 690
BmiI GGNNCC 1 cut(s) 692
BmsI GCATC 5 cut(s) 195, 254, 350, 511, 637
BpiI GAAGAC 1 cut(s) 693
BpmI CTGGAG 2 cut(s) 21, 289
Bpu10I CCTNAGC 1 cut(s) 664
Bpu1102I GCTNAGC 1 cut(s) 99
Bsa29I ATCGAT 1 cut(s) 735
BsaAI YACGTR 1 cut(s) 213
BsaJI CCNNGG 3 cut(s) 286, 686, 776
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 661, 691
Bsc4I CCNNNNNNNGG 1 cut(s) 658
Bse1I ACTGG 1 cut(s) 190
Bse3DI GCAATG 1 cut(s) 557
BseCI ATCGAT 1 cut(s) 735
BseDI CCNNGG 3 cut(s) 286, 686, 776
BseGI GGATG 3 cut(s) 186, 341, 538
BseLI CCNNNNNNNGG 1 cut(s) 658
BseMI GCAATG 1 cut(s) 557
BseMII CTCAG 4 cut(s) 113, 155, 657, 678
BseNI ACTGG 1 cut(s) 190
BseRI GAGGAG 1 cut(s) 716
BseXI GCAGC 3 cut(s) 115, 217, 406
BsgI GTGCAG 1 cut(s) 349
BshFI GGCC 2 cut(s) 595, 662
BshVI ATCGAT 1 cut(s) 735
BsiHKAI GWGCWC 1 cut(s) 100
BslI CCNNNNNNNGG 1 cut(s) 658
BsnI GGCC 2 cut(s) 595, 662
Bsp1286I GDGCHC 2 cut(s) 100, 751
Bsp1407I TGTACA 1 cut(s) 108
Bsp143I GATC 2 cut(s) 713, 766
Bsp1720I GCTNAGC 1 cut(s) 99
BspACI CCGC 3 cut(s) 449, 605, 744
BspANI GGCC 2 cut(s) 595, 662
BspCNI CTCAG 4 cut(s) 112, 154, 656, 677
BspDI ATCGAT 1 cut(s) 735
BspLI GGNNCC 1 cut(s) 692
BspMI ACCTGC 1 cut(s) 196
BspQI GCTCTTC 1 cut(s) 484
BsrDI GCAATG 1 cut(s) 557
BsrGI TGTACA 1 cut(s) 108
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 3 cut(s) 286, 686, 776
BssMI GATC 2 cut(s) 713, 766
BssNAI GTATAC 1 cut(s) 150
Bst1107I GTATAC 1 cut(s) 150
Bst4CI ACNGT 3 cut(s) 333, 687, 780
Bst6I CTCTTC 1 cut(s) 484
BstAUI TGTACA 1 cut(s) 108
BstBAI YACGTR 1 cut(s) 213
BstC8I GCNNGC 3 cut(s) 184, 300, 453
BstDEI CTNAG 4 cut(s) 99, 141, 643, 664
BstDSI CCRYGG 2 cut(s) 686, 776
BstF5I GGATG 3 cut(s) 186, 341, 538
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 2 cut(s) 716, 769
BstMBI GATC 2 cut(s) 713, 766
BstMWI GCNNNNNNNGC 5 cut(s) 227, 308, 333, 425, 613
BstNSI RCATGY 1 cut(s) 186
BstV1I GCAGC 3 cut(s) 115, 217, 406
BstV2I GAAGAC 1 cut(s) 693
BstX2I RGATCY 2 cut(s) 713, 766
BstYI RGATCY 2 cut(s) 713, 766
BstZ17I GTATAC 1 cut(s) 150
Bsu15I ATCGAT 1 cut(s) 735
BsuI GTATCC 1 cut(s) 238
BsuRI GGCC 2 cut(s) 595, 662
BsuTUI ATCGAT 1 cut(s) 735
BtgI CCRYGG 2 cut(s) 686, 776
BtsCI GGATG 3 cut(s) 186, 341, 538
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 2 cut(s) 29, 338
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 3 cut(s) 184, 300, 453
CaiI CAGNNNCTG 1 cut(s) 106
CfoI GCGC 1 cut(s) 311
Cfr13I GGNCC 1 cut(s) 690
ClaI ATCGAT 1 cut(s) 735
Csp6I GTAC 2 cut(s) 109, 409
CviAII CATG 2 cut(s) 183, 200
CviQI GTAC 2 cut(s) 109, 409
DdeI CTNAG 4 cut(s) 99, 141, 643, 664
DpnI GATC 2 cut(s) 715, 768
DpnII GATC 2 cut(s) 713, 766
DrdI GACNNNNNNGTC 1 cut(s) 697
DseDI GACNNNNNNGTC 1 cut(s) 697
Eam1104I CTCTTC 1 cut(s) 484
EarI CTCTTC 1 cut(s) 484
EciI GGCGGA 1 cut(s) 594
Ecl136II GAGCTC 1 cut(s) 98
Eco147I AGGCCT 1 cut(s) 595
Eco24I GRGCYC 2 cut(s) 100, 751
Eco47I GGWCC 1 cut(s) 690
Eco53kI GAGCTC 1 cut(s) 98
Eco57I CTGAAG 2 cut(s) 155, 174
EcoICRI GAGCTC 1 cut(s) 98
EcoT22I ATGCAT 1 cut(s) 188
EcoT38I GRGCYC 2 cut(s) 100, 751
FaeI CATG 2 cut(s) 186, 203
FaiI YATR 8 cut(s) 93, 132, 150, 184, 201, 273, 740, 742
FatI CATG 2 cut(s) 182, 199
FauI CCCGC 1 cut(s) 737
FblI GTMKAC 1 cut(s) 149
Fnu4HI GCNGC 4 cut(s) 104, 231, 420, 449
FokI GGATG 3 cut(s) 173, 328, 525
FriOI GRGCYC 2 cut(s) 100, 751
Fsp4HI GCNGC 4 cut(s) 104, 231, 420, 449
FspBI CTAG 2 cut(s) 348, 356
GlaI GCGC 1 cut(s) 310
GluI GCNGC 4 cut(s) 104, 231, 420, 449
GsuI CTGGAG 2 cut(s) 21, 289
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 2 cut(s) 595, 662
HhaI GCGC 1 cut(s) 311
Hin1II CATG 2 cut(s) 186, 203
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HindIII AAGCTT 1 cut(s) 156
HinfI GANTC 1 cut(s) 374
HphI GGTGA 1 cut(s) 675
Hpy166II GTNNAC 2 cut(s) 150, 690
Hpy188I TCNGA 2 cut(s) 144, 646
Hpy188III TCNNGA 2 cut(s) 518, 542
Hpy8I GTNNAC 2 cut(s) 150, 690
Hpy99I CGWCG 1 cut(s) 661
HpyAV CCTTC 5 cut(s) 74, 408, 499, 606, 752
HpyCH4III ACNGT 3 cut(s) 333, 687, 780
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 6 cut(s) 182, 186, 336, 366, 428, 524
HpyF10VI GCNNNNNNNGC 5 cut(s) 227, 308, 333, 425, 613
HpyF3I CTNAG 4 cut(s) 99, 141, 643, 664
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 2 cut(s) 186, 203
HspAI GCGC 1 cut(s) 309
Kzo9I GATC 2 cut(s) 713, 766
LguI GCTCTTC 1 cut(s) 484
LmnI GCTCC 2 cut(s) 95, 471
LpnPI CCDG 7 cut(s) 9, 51, 191, 203, 319, 363, 406
Lsp1109I GCAGC 3 cut(s) 115, 217, 406
LweI GCATC 5 cut(s) 195, 254, 350, 511, 637
MaeI CTAG 2 cut(s) 348, 356
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 3 cut(s) 83, 208, 681
MalI GATC 2 cut(s) 715, 768
MboI GATC 2 cut(s) 713, 766
MboII GAAGA 3 cut(s) 161, 471, 693
MflI RGATCY 2 cut(s) 713, 766
MhlI GDGCHC 2 cut(s) 100, 751
MluCI AATT 2 cut(s) 51, 59
MmeI TCCRAC 1 cut(s) 426
Mph1103I ATGCAT 1 cut(s) 188
MslI CAYNNNNRTG 1 cut(s) 778
MspA1I CMGCKG 1 cut(s) 106
MwoI GCNNNNNNNGC 5 cut(s) 227, 308, 333, 425, 613
NdeII GATC 2 cut(s) 713, 766
NlaIII CATG 2 cut(s) 186, 203
NlaIV GGNNCC 1 cut(s) 692
NmuCI GTSAC 1 cut(s) 681
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 1 cut(s) 186
OliI CACNNNNGTG 1 cut(s) 778
PaeI GCATGC 1 cut(s) 186
PceI AGGCCT 1 cut(s) 595
PciSI GCTCTTC 1 cut(s) 484
PcsI WCGNNNNNNNCGW 1 cut(s) 665
PfeI GAWTC 1 cut(s) 374
PkrI GCNGC 4 cut(s) 105, 232, 421, 450
Ppu21I YACGTR 1 cut(s) 213
Psp124BI GAGCTC 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 692
PspPI GGNCC 1 cut(s) 690
PstNI CAGNNNCTG 1 cut(s) 106
PsuI RGATCY 2 cut(s) 713, 766
PvuII CAGCTG 1 cut(s) 106
RsaI GTAC 2 cut(s) 110, 410
RsaNI GTAC 2 cut(s) 109, 409
RseI CAYNNNNRTG 1 cut(s) 778
SacI GAGCTC 1 cut(s) 100
SapI GCTCTTC 1 cut(s) 484
SatI GCNGC 4 cut(s) 104, 231, 420, 449
Sau3AI GATC 2 cut(s) 713, 766
Sau96I GGNCC 1 cut(s) 690
SduI GDGCHC 2 cut(s) 100, 751
SfaNI GCATC 5 cut(s) 195, 254, 350, 511, 637
SinI GGWCC 1 cut(s) 690
SmiMI CAYNNNNRTG 1 cut(s) 778
SphI GCATGC 1 cut(s) 186
Sse9I AATT 2 cut(s) 51, 59
SseBI AGGCCT 1 cut(s) 595
SsiI CCGC 3 cut(s) 449, 605, 744
SspMI CTAG 2 cut(s) 348, 356
SstI GAGCTC 1 cut(s) 100
StuI AGGCCT 1 cut(s) 595
TaaI ACNGT 3 cut(s) 333, 687, 780
TaiI ACGT 1 cut(s) 215
TaqI TCGA 5 cut(s) 5, 629, 656, 735, 756
TaqII GACCGA 1 cut(s) 278
TasI AATT 2 cut(s) 51, 59
TatI WGTACW 2 cut(s) 108, 408
TauI GCSGC 1 cut(s) 451
TfiI GAWTC 1 cut(s) 374
TscAI CASTG 2 cut(s) 36, 338
TseFI GTSAC 1 cut(s) 681
TseI GCWGC 3 cut(s) 103, 230, 419
Tsp45I GTSAC 1 cut(s) 681
TspDTI ATGAA 2 cut(s) 294, 663
TspGWI ACGGA 2 cut(s) 458, 669
TspRI CASTG 2 cut(s) 36, 338
VpaK11BI GGWCC 1 cut(s) 690
XapI RAATTY 1 cut(s) 59
XceI RCATGY 1 cut(s) 186
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 149
XspI CTAG 2 cut(s) 348, 356
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.