Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0006120

Physical Location & Seq

Genomic Coordinates Forward (+)

78026466 .. 78031884

Gene Structure 5419 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2559 bp

ATGGAATTGCCAGACTACTCATTGGTTAAGAATCTTGTAATGAAGGTTAAAAAGGAGATGTTCTCATCTTCATCTTCATCATCAGAACATGATGATGTATATCGTCTTGTGTCTCCTTCTGCTTACGACACTGCTTGGCTCGCCATGGTTCCGAATCCTGAAAAACCAGATCGGCCCTTGTTCCGGGGATGCTTGCAATGGGTGCTCCGGAACCAAAAACCAGGAGGCTTCTGGGGATCATCAGAGTCGACCTCTATAAGTACCTTATACTCCCTCACTTCGACTCTCGCTTGCATTGTTGCACTCACCACCTGGAGTGTTGGCCACACTGCCATTCAAAAGGGGTTGGGGTTCATTCATGCAAATGCAGAAGAGATACTGAAAGAGCAAAATGGCTGCTTTCCCGAGTGGTTTGTACTTCTTTTACCGGCAATAGTTGACCTTGCAAAGACCAAAGGCCTGCATGTTCATTTCTCAAATGGTTCAAAAGCGCTTATACAACAGATCTTCCATGAGAGACAACAGCTATTCCAAATCAACAGGTCGGGACAGGAGTACTATGAGACACTGATGCCATATCTTGAAGCTTTGCCGGAGAGTTATAATGAGATTTTGCTACAACAGAGTGAAGATTATGGGATCCTCATCCAGTTTCCATCAGCCACCGCCTATGCTTTTATGAAGACAGGAAATAAGAATCTTTTGGTGAATTTGAATTCTGTTGTCCAAAGATGTGGATATGGAGTTCCTGCGATTTACCCCATGGATGAAGACCTCGTAAAGATTTGTCTTATTAACCGGATTGAGACACTGGGCCTAGCAGAGTATTTCATGGAGGAGATAACGATTCTACTTGATCAAGTTTTCAGTTATGTGAACAATGAAGGGACAGAGATGACGGTGAAGCATGGTCTGCTTCCACTAGAACTTTATAGAGATTCACTAGCATTTCGGCTACTAAGACTACATGGCTATACTGTATCTCCTTGGAAGTTCTGCTGGTTCCTCCAAGATGAAGATATATTGGTGCACATAGAAGAGCACCATGAAACGTTTTTGAGTGCCATGTATAATGTATTCAGGGCATCAGATCTTACTTTTGCCGGAGAAAGTCAGCTTGAAGATGCTAGGTTATTCTCTACAAGAATACTGGAAAAGGAAACAACGAATTCCGAGAAATTGCTAGTAGACCAGATTAAGCATGAACTTAGTCATCCATGGCTTGCTCGGTTGGATCATTTGGAACACAGGAAATGCATTGAGAGGGAAGATGCTATTCATCCATGGACAGGAAAAGCCTTTTCGTATAGACTATCATTTTCGAGTGATGCTCTGCTGCAACAACTTGCCATAGAGAATTATTCACTCAGACAATCAATATTCAGAGATGAACTAACCGAGTTAGAGAGGTGGTCAAAGGACATGGGACTTTGTGACATGGGGTTTGCACGACAGAAAACCGCACACTGTTATTTTGCAGTTGCATCAACTGTATCACCTCCTTCCCTTTCGGATGTGAGGCTGGCACTTGTCAAAAGTGCAATCCTTGTTACGGTGGCTGATGATTTTTTTGATGCGGAAGGTTCATTGAGTGATCTCAAAAGTTTTACAAATGCCGTCAAAAGACGTCAGCAGATAGGCCTGAATGGACACAGCAAAACTATATTTAAAGCTCTCAAGGATCTCCTCGATGACATTTCTATGCAATTCTTCAATAAATATGGATATGACGTAAACATATACCTTCAAGATCTATGGCATCAAACATTTGTGAAATGGTTAAAGGAGGCAGAGTGGAGCAGTACTGGGCATACCCCATCAATAGCTGAGTACCTTCAAGTAGCCACGAGTTCAATAGCTGCACAAACTATTATTCTTCCAGCAGCTCTTCTTCTTAGTCCACAACCCGGAATAGACATCCTCAAGTGCCCTCAAAATGAGCCGCTTACAAATTTACTTATGGTTTCAACACGCTTTTTGAACGATATTGGAACCTATAAGCGGGAACAAGAGGAGGGGAAGCCAAACCTTGTTCTGCTACACATGAAGGAAAATCCGGATCTGGGAGTTGAAGAATCAATTGAGGTTATACAGAAGATATTGGACGAAAAGAAGAAACAGTTCCTGGAACAGGTTTTGGTGAATGGAGTGAATGATATGCCAGAAGCATGTAAACTACTCCACTTTCAATGCCTGAAAGCATTTCAGATGTTCTACAACTCAACAAATGCATTTGACTCCCAAACAGAGTTGCTTGCTGACATCAACAAAGCGATTTATTTTCCATTAAAGGTGGATCATAGAAGTTTGGCTAGTAGCCATAGCCAAGTTGGTACTAAAAGCCTTCAAATCCAACAGAGAGTAATTGGAACAAGTTCAACATTCTTGAAGAGCATTACTGAGAACAGAACTTGTACTACTGGTGCTATAAAGTTCCTCCAAGGAAAGAAGTTTTCTGCAAAGCCTTACGCGGGGATCTTATCACTTAAGATGAGAAAATCAATAACTCCTACGACTTTCCCAAAGCCGAAAGCTTGTACTTATTCGTGGCATAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0000304 GO:0001101 GO:0003674 GO:0003824 GO:0006629 GO:0006720 GO:0006721 GO:0006950 GO:0006979 GO:0007584 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0016101 GO:0016102 GO:0016114 GO:0016829 GO:0016835 GO:0016838 GO:0031667 GO:0042221 GO:0042493 GO:0043207 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0080013 GO:0080027 GO:1901576 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00904 map00904

Pfam Domains

Protein Families

Terpene_synth Terpene_synth_C

Protein Analysis

852

Amino Acids

96.91

Weight (kDa)

6.11

Isoelectric Point (pI)

45.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Terpene_synth	PF01397	219 - 403	1.1e-30	Terpene synthase, N-terminal domain
Terpene_synth_C	PF03936	473 - 706	1.5e-52	Terpene synthase family, metal binding domain
Terpene_syn_C_2	PF19086	513 - 706	1.7e-35	Terpene synthase family 2, C-terminal metal binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006120)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61120
malus_domestica	MD17G1080300.v1.1 MD17G1080400.v1.1 MD17G1080500.v1.1 MD17G1080700.v1.1 MD17G1080800.v1.1
prunus_persica	Prupe.3G234300_v2.0.a1 Prupe.3G234400_v2.0.a1 Prupe.3G234400_v2.0.a1 Prupe.3G234500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0162311
rosa_laevigata	RLG00000021334 RLG00000021337
rosa_multiflora	Rmu_co8462281.1_g000001 Rmu_sc0004020.1_g000013
rosa_roxburghii	Rroxscaffold_2G00088040 Rroxscaffold_2G00088060 Rroxscaffold_2G00088110
rosa_rugosa	Rorug02G0496200 Rorug02G0496300
rosa_samantha	Rh2AG562300 Rh2AG562400 Rh2BG575400 Rh2CG545800 Rh2DG585200 Rh2DG585600
rosa_wichuraiana	Rw2G046570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	603
AatII	GACGTC	1 cut(s)	1630
AccI	GTMKAC	2 cut(s)	248, 1188
AccII	CGCG	1 cut(s)	2471
AccIII	TCCGGA	2 cut(s)	207, 2056
AciI	CCGC	6 cut(s)	666, 1461, 1577, 1943, 2002, 2471
AclI	AACGTT	1 cut(s)	1052
AclWI	GGATC	8 cut(s)	244, 634, 647, 1242, 1689, 2067, 2304, 2483
AcoI	YGGCCR	1 cut(s)	322
AcsI	RAATTY	4 cut(s)	709, 715, 1168, 1951
AcyI	GRCGYC	1 cut(s)	1627
AfaI	GTAC	8 cut(s)	262, 417, 557, 1804, 1832, 2335, 2416, 2539
AfeI	AGCGCT	1 cut(s)	492
AfiI	CCNNNNNNNGG	8 cut(s)	183, 1289, 1552, 1907, 2001, 2063, 2131, 2471
AflII	CTTAAG	1 cut(s)	2486
AjnI	CCWGG	3 cut(s)	220, 311, 2124
AloI	GAACNNNNNNTCC	2 cut(s)	729, 761
AluBI	AGCT	8 cut(s)	526, 587, 1117, 1673, 1826, 1859, 1886, 2534
AluI	AGCT	8 cut(s)	526, 587, 1117, 1673, 1826, 1859, 1886, 2534
Alw21I	GWGCWC	3 cut(s)	207, 1032, 1044
Alw26I	GTCTC	4 cut(s)	117, 511, 557, 800
Alw44I	GTGCAC	1 cut(s)	1028
AlwI	GGATC	8 cut(s)	244, 634, 647, 1242, 1689, 2067, 2304, 2483
AlwNI	CAGNNNCTG	1 cut(s)	2125
Ama87I	CYCGRG	1 cut(s)	404
Aor13HI	TCCGGA	2 cut(s)	207, 2056
Aor51HI	AGCGCT	1 cut(s)	492
AoxI	GGCC	5 cut(s)	173, 322, 457, 814, 1639
ApaLI	GTGCAC	1 cut(s)	1028
ApeKI	GCWGC	4 cut(s)	396, 1336, 1859, 1883
ApoI	RAATTY	4 cut(s)	709, 715, 1168, 1951
ArsI	GACNNNNNNTTYG	2 cut(s)	1427, 1459
Asp700I	GAANNNNTTC	2 cut(s)	2120, 2374
AspLEI	GCGC	1 cut(s)	493
AspS9I	GGNCC	2 cut(s)	174, 814
AsuC2I	CCSGG	2 cut(s)	185, 1908
AsuHPI	GGTGA	5 cut(s)	298, 718, 913, 1488, 2152
AvaI	CYCGRG	1 cut(s)	404
BaeGI	GKGCMC	2 cut(s)	1032, 1931
BalI	TGGCCA	1 cut(s)	324
BamHI	GGATCC	1 cut(s)	639
BauI	CACGAG	1 cut(s)	1846
BbsI	GAAGAC	2 cut(s)	689, 777
Bbv12I	GWGCWC	3 cut(s)	207, 1032, 1044
BbvI	GCAGC	4 cut(s)	383, 1323, 1846, 1895
BccI	CCATC	2 cut(s)	664, 1825
BceAI	ACGGC	1 cut(s)	1601
BciT130I	CCWGG	3 cut(s)	222, 313, 2126
BclI	TGATCA	1 cut(s)	856
BcnI	CCSGG	2 cut(s)	185, 1908
BcoDI	GTCTC	4 cut(s)	117, 511, 557, 800
BfaI	CTAG	6 cut(s)	818, 923, 944, 1128, 1184, 2313
BfoI	RGCGCY	1 cut(s)	494
BfrI	CTTAAG	1 cut(s)	2486
BglII	AGATCT	3 cut(s)	504, 1090, 1750
BisI	GCNGC	5 cut(s)	397, 1337, 1860, 1884, 1943
BlsI	GCNGC	5 cut(s)	398, 1338, 1861, 1885, 1944
BmcAI	AGTACT	2 cut(s)	557, 1804
Bme1390I	CCNGG	5 cut(s)	185, 222, 313, 1908, 2126
BmeT110I	CYCGRG	1 cut(s)	404
BmgT120I	GGNCC	2 cut(s)	174, 814
BmiI	GGNNCC	5 cut(s)	150, 212, 641, 1004, 1993
BmrFI	CCNGG	5 cut(s)	185, 222, 313, 1908, 2126
BmrI	ACTGGG	2 cut(s)	821, 1815
BmsI	GCATC	9 cut(s)	179, 561, 1094, 1114, 1261, 1318, 1493, 1564, 1768
BmuI	ACTGGG	2 cut(s)	821, 1815
BpiI	GAAGAC	2 cut(s)	689, 777
BplI	GAGNNNNNCTC	6 cut(s)	47, 79, 236, 268, 1315, 1347
BpmI	CTGGAG	1 cut(s)	334
BpuEI	CTTGAG	2 cut(s)	1661, 1907
BpuMI	CCSGG	2 cut(s)	185, 1908
BsaBI	GATNNNNATC	2 cut(s)	99, 644
BsaHI	GRCGYC	1 cut(s)	1627
BsaJI	CCNNGG	7 cut(s)	144, 184, 762, 986, 1217, 1283, 2440
BsaWI	WCCGGW	3 cut(s)	207, 798, 2056
BsaXI	ACNNNNNCTCC	4 cut(s)	967, 997, 1778, 1808
Bsc4I	CCNNNNNNNGG	8 cut(s)	183, 1289, 1552, 1907, 2001, 2063, 2131, 2471
Bse118I	RCCGGY	1 cut(s)	427
Bse1I	ACTGG	5 cut(s)	649, 816, 1155, 1810, 2425
Bse3DI	GCAATG	1 cut(s)	203
Bse8I	GATNNNNATC	2 cut(s)	99, 644
BseAI	TCCGGA	2 cut(s)	207, 2056
BseBI	CCWGG	3 cut(s)	222, 313, 2126
BseDI	CCNNGG	7 cut(s)	144, 184, 762, 986, 1217, 1283, 2440
BseGI	GGATG	7 cut(s)	194, 645, 772, 1213, 1279, 1519, 1917
BseJI	GATNNNNATC	2 cut(s)	99, 644
BseLI	CCNNNNNNNGG	8 cut(s)	183, 1289, 1552, 1907, 2001, 2063, 2131, 2471
BseMI	GCAATG	1 cut(s)	203
BseMII	CTCAG	3 cut(s)	1381, 1818, 2391
BseNI	ACTGG	5 cut(s)	649, 816, 1155, 1810, 2425
BseRI	GAGGAG	3 cut(s)	851, 1676, 2027
BseSI	GKGCMC	2 cut(s)	1032, 1931
BseXI	GCAGC	4 cut(s)	383, 1323, 1846, 1895
BsgI	GTGCAG	1 cut(s)	1845
Bsh1236I	CGCG	1 cut(s)	2471
BshFI	GGCC	5 cut(s)	175, 324, 459, 816, 1641
BsiHKAI	GWGCWC	3 cut(s)	207, 1032, 1044
BsiHKCI	CYCGRG	1 cut(s)	404
BsiSI	CCGG	8 cut(s)	184, 208, 428, 593, 799, 1104, 1908, 2057
BslFI	GGGAC	3 cut(s)	561, 901, 1440
BslI	CCNNNNNNNGG	8 cut(s)	183, 1289, 1552, 1907, 2001, 2063, 2131, 2471
BsmAI	GTCTC	4 cut(s)	117, 511, 557, 800
BsmFI	GGGAC	3 cut(s)	561, 901, 1440
BsnI	GGCC	5 cut(s)	175, 324, 459, 816, 1641
BsoBI	CYCGRG	1 cut(s)	404
Bsp1286I	GDGCHC	4 cut(s)	207, 1032, 1044, 1931
Bsp13I	TCCGGA	2 cut(s)	207, 2056
Bsp19I	CCATGG	4 cut(s)	144, 762, 1217, 1283
BspACI	CCGC	6 cut(s)	666, 1461, 1577, 1943, 2002, 2471
BspANI	GGCC	5 cut(s)	175, 324, 459, 816, 1641
BspCNI	CTCAG	3 cut(s)	1380, 1819, 2392
BspEI	TCCGGA	2 cut(s)	207, 2056
BspFNI	CGCG	1 cut(s)	2471
BspLI	GGNNCC	5 cut(s)	150, 212, 641, 1004, 1993
BspPI	GGATC	8 cut(s)	244, 634, 647, 1242, 1689, 2067, 2304, 2483
BspQI	GCTCTTC	3 cut(s)	1032, 1893, 2384
BspTI	CTTAAG	1 cut(s)	2486
BsrDI	GCAATG	1 cut(s)	203
BsrFI	RCCGGY	1 cut(s)	427
BsrI	ACTGG	5 cut(s)	649, 816, 1155, 1810, 2425
BssAI	RCCGGY	1 cut(s)	427
BssECI	CCNNGG	7 cut(s)	144, 184, 762, 986, 1217, 1283, 2440
BssNI	GRCGYC	1 cut(s)	1627
BssSI	CACGAG	1 cut(s)	1846
BssT1I	CCWWGG	6 cut(s)	144, 762, 986, 1217, 1283, 2440
Bst2BI	CACGAG	1 cut(s)	1846
Bst2UI	CCWGG	3 cut(s)	222, 313, 2126
Bst4CI	ACNGT	6 cut(s)	901, 979, 1469, 1492, 1555, 2121
Bst6I	CTCTTC	4 cut(s)	366, 1032, 1893, 2384
BstACI	GRCGYC	1 cut(s)	1627
BstAFI	CTTAAG	1 cut(s)	2486
BstAPI	GCANNNNNTGC	2 cut(s)	202, 913
BstC8I	GCNNGC	7 cut(s)	141, 194, 292, 461, 1224, 1524, 2256
BstDEI	CTNAG	6 cut(s)	959, 1208, 1367, 1827, 1895, 2400
BstDSI	CCRYGG	4 cut(s)	144, 762, 1217, 1283
BstENI	CCTNNNNNAGG	1 cut(s)	2129
BstF5I	GGATG	7 cut(s)	194, 645, 772, 1213, 1279, 1519, 1917
BstFNI	CGCG	1 cut(s)	2471
BstH2I	RGCGCY	1 cut(s)	494
BstHHI	GCGC	1 cut(s)	493
BstMAI	GTCTC	4 cut(s)	117, 511, 557, 800
BstMWI	GCNNNNNNNGC	3 cut(s)	140, 202, 913
BstNI	CCWGG	3 cut(s)	222, 313, 2126
BstNSI	RCATGY	2 cut(s)	467, 2172
BstSCI	CCNGG	5 cut(s)	183, 220, 311, 1906, 2124
BstSLI	GKGCMC	2 cut(s)	1032, 1931
BstUI	CGCG	1 cut(s)	2471
BstV1I	GCAGC	4 cut(s)	383, 1323, 1846, 1895
BstV2I	GAAGAC	2 cut(s)	689, 777
BstX2I	RGATCY	7 cut(s)	504, 639, 1090, 1681, 1750, 2059, 2475
BstXI	CCANNNNNNTGG	1 cut(s)	734
BstYI	RGATCY	7 cut(s)	504, 639, 1090, 1681, 1750, 2059, 2475
BsuRI	GGCC	5 cut(s)	175, 324, 459, 816, 1641
BtgI	CCRYGG	4 cut(s)	144, 762, 1217, 1283
BtsCI	GGATG	7 cut(s)	194, 645, 772, 1213, 1279, 1519, 1917
BtsI	GCAGTG	2 cut(s)	129, 327
BtsIMutI	CAGTG	5 cut(s)	129, 327, 566, 809, 1465
Cac8I	GCNNGC	7 cut(s)	141, 194, 292, 461, 1224, 1524, 2256
CaiI	CAGNNNCTG	1 cut(s)	2125
CfoI	GCGC	1 cut(s)	493
Cfr10I	RCCGGY	1 cut(s)	427
Cfr13I	GGNCC	2 cut(s)	174, 814
Csp6I	GTAC	8 cut(s)	261, 416, 556, 1803, 1831, 2334, 2415, 2538
CviQI	GTAC	8 cut(s)	261, 416, 556, 1803, 1831, 2334, 2415, 2538
DdeI	CTNAG	6 cut(s)	959, 1208, 1367, 1827, 1895, 2400
DraI	TTTAAA	1 cut(s)	1669
EaeI	YGGCCR	1 cut(s)	322
Eam1104I	CTCTTC	4 cut(s)	366, 1032, 1893, 2384
EarI	CTCTTC	4 cut(s)	366, 1032, 1893, 2384
Eco130I	CCWWGG	6 cut(s)	144, 762, 986, 1217, 1283, 2440
Eco147I	AGGCCT	2 cut(s)	459, 1641
Eco47III	AGCGCT	1 cut(s)	492
Eco88I	CYCGRG	1 cut(s)	404
EcoNI	CCTNNNNNAGG	1 cut(s)	2129
EcoRI	GAATTC	2 cut(s)	715, 1168
EcoRII	CCWGG	3 cut(s)	220, 311, 2124
EcoT14I	CCWWGG	6 cut(s)	144, 762, 986, 1217, 1283, 2440
EcoT22I	ATGCAT	2 cut(s)	1259, 2233
ErhI	CCWWGG	6 cut(s)	144, 762, 986, 1217, 1283, 2440
FaqI	GGGAC	3 cut(s)	561, 901, 1440
FauI	CCCGC	2 cut(s)	1995, 2464
FbaI	TGATCA	1 cut(s)	856
FblI	GTMKAC	2 cut(s)	248, 1188
Fnu4HI	GCNGC	5 cut(s)	397, 1337, 1860, 1884, 1943
FokI	GGATG	7 cut(s)	201, 632, 779, 1200, 1266, 1526, 1904
Fsp4HI	GCNGC	5 cut(s)	397, 1337, 1860, 1884, 1943
FspBI	CTAG	6 cut(s)	818, 923, 944, 1128, 1184, 2313
GlaI	GCGC	1 cut(s)	492
GluI	GCNGC	5 cut(s)	397, 1337, 1860, 1884, 1943
GsuI	CTGGAG	1 cut(s)	334
HaeII	RGCGCY	1 cut(s)	494
HaeIII	GGCC	5 cut(s)	175, 324, 459, 816, 1641
HapII	CCGG	8 cut(s)	184, 208, 428, 593, 799, 1104, 1908, 2057
HhaI	GCGC	1 cut(s)	493
Hin1I	GRCGYC	1 cut(s)	1627
Hin6I	GCGC	1 cut(s)	491
HinP1I	GCGC	1 cut(s)	491
HincII	GTYRAC	2 cut(s)	249, 439
HindII	GTYRAC	2 cut(s)	249, 439
HindIII	AAGCTT	2 cut(s)	585, 2532
HinfI	GANTC	9 cut(s)	31, 154, 245, 283, 697, 847, 938, 2075, 2237
HpaII	CCGG	8 cut(s)	184, 208, 428, 593, 799, 1104, 1908, 2057
HphI	GGTGA	5 cut(s)	298, 718, 913, 1488, 2152
Hpy166II	GTNNAC	8 cut(s)	249, 439, 877, 1030, 1189, 1735, 1901, 2174
Hpy188I	TCNGA	9 cut(s)	85, 153, 244, 1090, 1174, 1370, 1385, 1513, 2208
Hpy188III	TCNNGA	8 cut(s)	158, 208, 404, 546, 581, 1748, 2057, 2386
Hpy8I	GTNNAC	8 cut(s)	249, 439, 877, 1030, 1189, 1735, 1901, 2174
HpyAV	CCTTC	9 cut(s)	37, 126, 878, 1512, 1574, 1754, 1844, 2041, 2354
HpyCH4III	ACNGT	6 cut(s)	901, 979, 1469, 1492, 1555, 2121
HpyCH4IV	ACGT	3 cut(s)	1052, 1627, 1731
HpyF10VI	GCNNNNNNNGC	3 cut(s)	140, 202, 913
HpyF3I	CTNAG	6 cut(s)	959, 1208, 1367, 1827, 1895, 2400
HpySE526I	ACGT	3 cut(s)	1052, 1627, 1731
Hsp92I	GRCGYC	1 cut(s)	1627
HspAI	GCGC	1 cut(s)	491
Kpn2I	TCCGGA	2 cut(s)	207, 2056
Ksp22I	TGATCA	1 cut(s)	856
LguI	GCTCTTC	3 cut(s)	1032, 1893, 2384
LmnI	GCTCC	2 cut(s)	210, 1797
Lsp1109I	GCAGC	4 cut(s)	383, 1323, 1846, 1895
LweI	GCATC	9 cut(s)	179, 561, 1094, 1114, 1261, 1318, 1493, 1564, 1768
MaeI	CTAG	6 cut(s)	818, 923, 944, 1128, 1184, 2313
MaeII	ACGT	3 cut(s)	1052, 1627, 1731
MaeIII	GTNAC	2 cut(s)	1433, 1549
MfeI	CAATTG	1 cut(s)	2079
MflI	RGATCY	7 cut(s)	504, 639, 1090, 1681, 1750, 2059, 2475
MhlI	GDGCHC	4 cut(s)	207, 1032, 1044, 1931
MlsI	TGGCCA	1 cut(s)	324
MluNI	TGGCCA	1 cut(s)	324
MlyI	GAGTC	3 cut(s)	254, 277, 2231
MmeI	TCCRAC	2 cut(s)	1212, 2377
Mox20I	TGGCCA	1 cut(s)	324
Mph1103I	ATGCAT	2 cut(s)	1259, 2233
MroI	TCCGGA	2 cut(s)	207, 2056
MroXI	GAANNNNTTC	2 cut(s)	2120, 2374
MscI	TGGCCA	1 cut(s)	324
MseI	TTAA	8 cut(s)	27, 48, 795, 1197, 1668, 1781, 2288, 2487
MslI	CAYNNNNRTG	3 cut(s)	93, 363, 1700
Msp20I	TGGCCA	1 cut(s)	324
MspCI	CTTAAG	1 cut(s)	2486
MspI	CCGG	8 cut(s)	184, 208, 428, 593, 799, 1104, 1908, 2057
MspR9I	CCNGG	5 cut(s)	185, 222, 313, 1908, 2126
MunI	CAATTG	1 cut(s)	2079
MvaI	CCWGG	3 cut(s)	222, 313, 2126
MvnI	CGCG	1 cut(s)	2471
MwoI	GCNNNNNNNGC	3 cut(s)	140, 202, 913
NciI	CCSGG	2 cut(s)	185, 1908
NcoI	CCATGG	4 cut(s)	144, 762, 1217, 1283
NlaIV	GGNNCC	5 cut(s)	150, 212, 641, 1004, 1993
NmuCI	GTSAC	1 cut(s)	1433
NsiI	ATGCAT	2 cut(s)	1259, 2233
NspI	RCATGY	2 cut(s)	467, 2172
PceI	AGGCCT	2 cut(s)	459, 1641
PciSI	GCTCTTC	3 cut(s)	1032, 1893, 2384
PdmI	GAANNNNTTC	2 cut(s)	2120, 2374
PfeI	GAWTC	6 cut(s)	31, 154, 697, 847, 938, 2075
PfoI	TCCNGGA	1 cut(s)	2124
PkrI	GCNGC	5 cut(s)	398, 1338, 1861, 1885, 1944
PleI	GAGTC	3 cut(s)	253, 277, 2231
PpsI	GAGTC	3 cut(s)	253, 277, 2231
PsiI	TTATAA	1 cut(s)	603
Psp1406I	AACGTT	1 cut(s)	1052
Psp6I	CCWGG	3 cut(s)	220, 311, 2124
PspGI	CCWGG	3 cut(s)	220, 311, 2124
PspN4I	GGNNCC	5 cut(s)	150, 212, 641, 1004, 1993
PspPI	GGNCC	2 cut(s)	174, 814
PsrI	GAACNNNNNNTAC	2 cut(s)	2401, 2433
PstNI	CAGNNNCTG	1 cut(s)	2125
PsuI	RGATCY	7 cut(s)	504, 639, 1090, 1681, 1750, 2059, 2475
RsaI	GTAC	8 cut(s)	262, 417, 557, 1804, 1832, 2335, 2416, 2539
RsaNI	GTAC	8 cut(s)	261, 416, 556, 1803, 1831, 2334, 2415, 2538
RseI	CAYNNNNRTG	3 cut(s)	93, 363, 1700
SalI	GTCGAC	1 cut(s)	247
SapI	GCTCTTC	3 cut(s)	1032, 1893, 2384
SaqAI	TTAA	8 cut(s)	27, 48, 795, 1197, 1668, 1781, 2288, 2487
SatI	GCNGC	5 cut(s)	397, 1337, 1860, 1884, 1943
Sau96I	GGNCC	2 cut(s)	174, 814
ScaI	AGTACT	2 cut(s)	557, 1804
SchI	GAGTC	3 cut(s)	254, 277, 2231
ScrFI	CCNGG	5 cut(s)	185, 222, 313, 1908, 2126
SduI	GDGCHC	4 cut(s)	207, 1032, 1044, 1931
SfaNI	GCATC	9 cut(s)	179, 561, 1094, 1114, 1261, 1318, 1493, 1564, 1768
SmiMI	CAYNNNNRTG	3 cut(s)	93, 363, 1700
SmlI	CTYRAG	3 cut(s)	1676, 1922, 2486
SmoI	CTYRAG	3 cut(s)	1676, 1922, 2486
SseBI	AGGCCT	2 cut(s)	459, 1641
SsiI	CCGC	6 cut(s)	666, 1461, 1577, 1943, 2002, 2471
SspI	AATATT	1 cut(s)	1380
SspMI	CTAG	6 cut(s)	818, 923, 944, 1128, 1184, 2313
StuI	AGGCCT	2 cut(s)	459, 1641
StyD4I	CCNGG	5 cut(s)	183, 220, 311, 1906, 2124
StyI	CCWWGG	6 cut(s)	144, 762, 986, 1217, 1283, 2440
TaaI	ACNGT	6 cut(s)	901, 979, 1469, 1492, 1555, 2121
TaiI	ACGT	3 cut(s)	1055, 1630, 1734
TaqI	TCGA	4 cut(s)	248, 281, 1322, 1689
TatI	WGTACW	5 cut(s)	415, 555, 1802, 2414, 2537
TauI	GCSGC	1 cut(s)	1945
TfiI	GAWTC	6 cut(s)	31, 154, 697, 847, 938, 2075
Tru1I	TTAA	8 cut(s)	27, 48, 795, 1197, 1668, 1781, 2288, 2487
Tru9I	TTAA	8 cut(s)	27, 48, 795, 1197, 1668, 1781, 2288, 2487
TscAI	CASTG	5 cut(s)	136, 334, 573, 816, 1472
TseFI	GTSAC	1 cut(s)	1433
TseI	GCWGC	4 cut(s)	396, 1336, 1859, 1883
Tsp45I	GTSAC	1 cut(s)	1433
TspRI	CASTG	5 cut(s)	136, 334, 573, 816, 1472
Vha464I	CTTAAG	1 cut(s)	2486
VneI	GTGCAC	1 cut(s)	1028
XagI	CCTNNNNNAGG	1 cut(s)	2129
XapI	RAATTY	4 cut(s)	709, 715, 1168, 1951
XceI	RCATGY	2 cut(s)	467, 2172
XcmI	CCANNNNNNNNNTGG	2 cut(s)	228, 2327
XmiI	GTMKAC	2 cut(s)	248, 1188
XmnI	GAANNNNTTC	2 cut(s)	2120, 2374
XspI	CTAG	6 cut(s)	818, 923, 944, 1128, 1184, 2313
ZraI	GACGTC	1 cut(s)	1628
ZrmI	AGTACT	2 cut(s)	557, 1804
Zsp2I	ATGCAT	2 cut(s)	1259, 2233

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.