Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0006120

Physical Location & Seq

Genomic Coordinates Forward (+)

79132889 .. 79138625

Gene Structure 5737 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG575400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2562 bp

ATGGAATTGCCAGACTACTCATTGGTTAAGAATCTTGTAATGAAGGTTAAAAAGGAGATGTTCTCATCTTCATCTTCATCATCAGAACATGATGATGTATATCGTCTTGTGTCTCCTTCTGCTTACGACACTGCTTGGCTCGCCATGGTTCCGAATCCTGAAAAACCAGATCGGCCCTTGTTCCGGGGATGCTTGCAATGGGTGCTCCGGAACCAAAAACCAGGAGGCTTCTGGGGATCATCAGAGTCGACCTCTATAAGTACCTTATACTCCCTCACTTCGACTCTCGCTTGCATTGTTGCACTCACCACCTGGAGTGTTGGCCACACTGCCATTCAAAAGGGGTTGGGGTTCATTCATGCAAATGCAGAAGAGATACTGAAAGAGCAAAATGGCTGCTTTCCCGAGTGGTTTGTACTTCTTTTACCGGCAATAGTTGACCTTGCAAAGACCAAAGGCCTGCATGTTCATTTCTCTTATGGTTCAAAAGCGCTTATACAACAGATCTTCCATGAGAGACAACATCTATTCCAAATCAACAGGTTGGGACAGGAGTACTATGAGACACTGATGCCATATCTTGAAGCTTTGCCGGAGAGTTATAATGAGATTTTGCTACAACAGAGTGAAGATTATGGGACCCTCATCCAGTTTCCATCAGCCACCGCCTATGCTTTTATGAAGACAGGAAATAAGAATCTTTTGGTGAATTTGAATTCTGTTGTCCAAAGATGTGGATATGGAGTTCCTGCGATTTACCCCATGGATGAAGACCTCGTAAAGATTTGTCTTATTAACCGGATTGAGACACTGGGCCTAGCAGAGTATTTCATGGAGGAGATCACGATTCTACTTGATCAAGTTTTCAGAAGTTATGTGAACAATGAAGGGACAGAGATGACGGTGAAGCATGGTCTGCTTCCACTAGAACTTTATAGAGATTCACTAGCATTTCGGCTACTGAGACTACATGGCTATACTGTATCTCCTTGGAAGTTCTGCTGGTTCCTCCAAGATGAAGATATATTGGTGCACATAGAAGAGCACCATGAAACGTTTTTGAGTGCCATGTATAATGTATTCAGGGCATCAGATCTTACTTTTGCCGGAGAAAGTCAGCTTGAAGATGCTAGGTTATTCTCTACAAGAATACTGGAAAAGGAAACAACAAATTCCGAGAAATTGCTAGTAGACCAGATTAAGCATGAACTTAGTCATCCATGGCTTGCTCGGTTGGATCATTTGGAACACAGGAAATGCATTGAGAAGGAAGATGCTATTCATCCATGGACAGGAAAAGCCTTTTCGTATAGACTATCATTTTCGAGTGATGCTCTGCTGCAACAACTTGCCATAGAGAATTATTCACTCAGACAATCAATATTCAGAGATGAACTAACCGAGTTAGAGAGGTGGTCAAAGGACATGGGACTTTGTGACATGGGGTTTGCACGACAGAAAACCGCACACTGTTATTTTGCAGTTGCATCAACTGTATCACCTCCTTCCCTTTCGGATGTGAGGCTGGCACTTGTCAAAAGTGCAATCCTTGTTACGGTGGCTGATGATTTTTTTGATGCGGAAGGTTCATTGAGTGATCTCAAAAGTTTTACAAATGCCGTCAAAAGACGTCAGCAGATAGGCCTGAATGGACACAGCAAAACTATATTTAAAGCTCTCAAGGATCTCCTCGATGACATTTCTATGCAATTCTTCAATAAATATGGATATGACGTAAACATATACCTTCAAGATCTATGGCATCAAACATTTGTGAAATGGTTAAAGGAGGCAGAGTGGAGCAGTACTGGGCATACCCCATCAATAGCTGAGTACCTTCAAGTAGCCACGAGTTCAATAGCTGCACAAACTATTATTCTTCCAGCAGCTCTTCTTCTTAGTCCACAACCCGGAATAGACATCCTCAAGTGCCCTCAAAATGAGCCGCTTACAAATTTACTTATGGTTTCAACACGCTTTTTGAACGATATTGGAACCTATAAGCGGGAACAAGAGGAGGGGAAGCCAAACCTTGTTCTGCTACACATGAAGGAAAATCCGGATCTGGGAGTTGAAGAATCAATTGAGGTTATACAGAAGATATTGGACGAAAAGAAGAAACAGTTCCTGGAACAGGTTTTGGTGAATGGAGTGAATGATATGCCAGAAGCATGTAAACTACTCCACTTTCAATGCCTGAAAGCATTTCAGATGTTCTACAACTCAACAAATGCATTTGACTCCCAAACAGAGTTGCTTGCTGACATCAACAAAGCGATTTATTTTCCATTAAAGGTGGATCATAGAAGTTTGGCTAGTAGCCATAGCCAAGTTGGTACTAAAAGCCTTCAAATCCAACAGAGAGTAATTGGAACAAGTTCAACATTCTCGAAGAGCATTACTGAGAACAGAACTTGTACTACTGGTGCTATAAAGTTCCTCCAAGGAAAGAAGTTTTCTGCAAAGCCTTACGCGGGGATCTTATCACTTAAGATGAGAAAATCAATAACTCCTACGACTTTCCCAAAGCCGAAAGCTTGTACTTATTCGTGGCTTAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0000304 GO:0001101 GO:0003674 GO:0003824 GO:0006629 GO:0006720 GO:0006721 GO:0006950 GO:0006979 GO:0007584 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0016101 GO:0016102 GO:0016114 GO:0016829 GO:0016835 GO:0016838 GO:0031667 GO:0042221 GO:0042493 GO:0043207 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0080013 GO:0080027 GO:1901576 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00904 map00904

Pfam Domains

Protein Families

Terpene_synth Terpene_synth_C

Protein Analysis

853

Amino Acids

97.06

Weight (kDa)

6.17

Isoelectric Point (pI)

44.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Terpene_synth	PF01397	219 - 404	6.6e-31	Terpene synthase, N-terminal domain
Terpene_synth_C	PF03936	474 - 707	1.5e-52	Terpene synthase family, metal binding domain
Terpene_syn_C_2	PF19086	514 - 707	1.7e-35	Terpene synthase family 2, C-terminal metal binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006120)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61120
malus_domestica	MD17G1080300.v1.1 MD17G1080400.v1.1 MD17G1080500.v1.1 MD17G1080700.v1.1 MD17G1080800.v1.1
prunus_persica	Prupe.3G234300_v2.0.a1 Prupe.3G234400_v2.0.a1 Prupe.3G234400_v2.0.a1 Prupe.3G234500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0162311
rosa_laevigata	RLG00000021334 RLG00000021337
rosa_multiflora	Rmu_co8462281.1_g000001 Rmu_sc0004020.1_g000013
rosa_roxburghii	Rroxscaffold_2G00088040 Rroxscaffold_2G00088060 Rroxscaffold_2G00088110
rosa_rugosa	Rorug02G0496200 Rorug02G0496300
rosa_samantha	Rh2AG562300 Rh2AG562400 Rh2BG575400 Rh2CG545800 Rh2DG585200 Rh2DG585600
rosa_wichuraiana	Rw2G046570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	603
AatII	GACGTC	1 cut(s)	1633
AccI	GTMKAC	2 cut(s)	248, 1191
AccII	CGCG	1 cut(s)	2474
AccIII	TCCGGA	2 cut(s)	207, 2059
AciI	CCGC	6 cut(s)	666, 1464, 1580, 1946, 2005, 2474
AclI	AACGTT	1 cut(s)	1055
AclWI	GGATC	6 cut(s)	244, 1245, 1692, 2070, 2307, 2486
AcoI	YGGCCR	1 cut(s)	322
AcsI	RAATTY	4 cut(s)	709, 715, 1171, 1954
AcyI	GRCGYC	1 cut(s)	1630
AfaI	GTAC	8 cut(s)	262, 417, 557, 1807, 1835, 2338, 2419, 2542
AfeI	AGCGCT	1 cut(s)	492
AfiI	CCNNNNNNNGG	8 cut(s)	183, 1292, 1555, 1910, 2004, 2066, 2134, 2474
AflII	CTTAAG	2 cut(s)	2489, 2555
AjnI	CCWGG	3 cut(s)	220, 311, 2127
AloI	GAACNNNNNNTCC	2 cut(s)	729, 761
AluBI	AGCT	7 cut(s)	587, 1120, 1676, 1829, 1862, 1889, 2537
AluI	AGCT	7 cut(s)	587, 1120, 1676, 1829, 1862, 1889, 2537
Alw21I	GWGCWC	3 cut(s)	207, 1035, 1047
Alw26I	GTCTC	5 cut(s)	117, 511, 557, 800, 958
Alw44I	GTGCAC	1 cut(s)	1031
AlwI	GGATC	6 cut(s)	244, 1245, 1692, 2070, 2307, 2486
AlwNI	CAGNNNCTG	1 cut(s)	2128
Ama87I	CYCGRG	1 cut(s)	404
Aor13HI	TCCGGA	2 cut(s)	207, 2059
Aor51HI	AGCGCT	1 cut(s)	492
AoxI	GGCC	5 cut(s)	173, 322, 457, 814, 1642
ApaLI	GTGCAC	1 cut(s)	1031
ApeKI	GCWGC	4 cut(s)	396, 1339, 1862, 1886
ApoI	RAATTY	4 cut(s)	709, 715, 1171, 1954
ArsI	GACNNNNNNTTYG	2 cut(s)	1430, 1462
Asp700I	GAANNNNTTC	2 cut(s)	2123, 2377
AspLEI	GCGC	1 cut(s)	493
AspS9I	GGNCC	3 cut(s)	174, 639, 814
AsuC2I	CCSGG	2 cut(s)	185, 1911
AsuHPI	GGTGA	5 cut(s)	298, 718, 916, 1491, 2155
AvaI	CYCGRG	1 cut(s)	404
AvaII	GGWCC	1 cut(s)	639
BaeGI	GKGCMC	2 cut(s)	1035, 1934
BalI	TGGCCA	1 cut(s)	324
BauI	CACGAG	1 cut(s)	1849
BbsI	GAAGAC	2 cut(s)	689, 777
Bbv12I	GWGCWC	3 cut(s)	207, 1035, 1047
BbvI	GCAGC	4 cut(s)	383, 1326, 1849, 1898
BccI	CCATC	2 cut(s)	664, 1828
BceAI	ACGGC	1 cut(s)	1604
BciT130I	CCWGG	3 cut(s)	222, 313, 2129
BclI	TGATCA	1 cut(s)	856
BcnI	CCSGG	2 cut(s)	185, 1911
BcoDI	GTCTC	5 cut(s)	117, 511, 557, 800, 958
BfaI	CTAG	6 cut(s)	818, 926, 947, 1131, 1187, 2316
BfoI	RGCGCY	1 cut(s)	494
BfrI	CTTAAG	2 cut(s)	2489, 2555
BglII	AGATCT	3 cut(s)	504, 1093, 1753
BisI	GCNGC	5 cut(s)	397, 1340, 1863, 1887, 1946
BlsI	GCNGC	5 cut(s)	398, 1341, 1864, 1888, 1947
BmcAI	AGTACT	2 cut(s)	557, 1807
Bme1390I	CCNGG	5 cut(s)	185, 222, 313, 1911, 2129
Bme18I	GGWCC	1 cut(s)	639
BmeT110I	CYCGRG	1 cut(s)	404
BmgT120I	GGNCC	3 cut(s)	174, 639, 814
BmiI	GGNNCC	6 cut(s)	150, 212, 640, 641, 1007, 1996
BmrFI	CCNGG	5 cut(s)	185, 222, 313, 1911, 2129
BmrI	ACTGGG	2 cut(s)	821, 1818
BmsI	GCATC	9 cut(s)	179, 561, 1097, 1117, 1264, 1321, 1496, 1567, 1771
BmuI	ACTGGG	2 cut(s)	821, 1818
BpiI	GAAGAC	2 cut(s)	689, 777
BplI	GAGNNNNNCTC	6 cut(s)	47, 79, 236, 268, 1318, 1350
BpmI	CTGGAG	1 cut(s)	334
BpuEI	CTTGAG	2 cut(s)	1664, 1910
BpuMI	CCSGG	2 cut(s)	185, 1911
BsaBI	GATNNNNATC	1 cut(s)	99
BsaHI	GRCGYC	1 cut(s)	1630
BsaJI	CCNNGG	7 cut(s)	144, 184, 762, 989, 1220, 1286, 2443
BsaWI	WCCGGW	3 cut(s)	207, 798, 2059
BsaXI	ACNNNNNCTCC	4 cut(s)	970, 1000, 1781, 1811
Bsc4I	CCNNNNNNNGG	8 cut(s)	183, 1292, 1555, 1910, 2004, 2066, 2134, 2474
Bse118I	RCCGGY	1 cut(s)	427
Bse1I	ACTGG	5 cut(s)	649, 816, 1158, 1813, 2428
Bse3DI	GCAATG	1 cut(s)	203
Bse8I	GATNNNNATC	1 cut(s)	99
BseAI	TCCGGA	2 cut(s)	207, 2059
BseBI	CCWGG	3 cut(s)	222, 313, 2129
BseDI	CCNNGG	7 cut(s)	144, 184, 762, 989, 1220, 1286, 2443
BseGI	GGATG	7 cut(s)	194, 645, 772, 1216, 1282, 1522, 1920
BseJI	GATNNNNATC	1 cut(s)	99
BseLI	CCNNNNNNNGG	8 cut(s)	183, 1292, 1555, 1910, 2004, 2066, 2134, 2474
BseMI	GCAATG	1 cut(s)	203
BseMII	CTCAG	4 cut(s)	953, 1384, 1821, 2394
BseNI	ACTGG	5 cut(s)	649, 816, 1158, 1813, 2428
BseRI	GAGGAG	3 cut(s)	851, 1679, 2030
BseSI	GKGCMC	2 cut(s)	1035, 1934
BseXI	GCAGC	4 cut(s)	383, 1326, 1849, 1898
BsgI	GTGCAG	1 cut(s)	1848
Bsh1236I	CGCG	1 cut(s)	2474
BshFI	GGCC	5 cut(s)	175, 324, 459, 816, 1644
BsiHKAI	GWGCWC	3 cut(s)	207, 1035, 1047
BsiHKCI	CYCGRG	1 cut(s)	404
BsiSI	CCGG	8 cut(s)	184, 208, 428, 593, 799, 1107, 1911, 2060
BslFI	GGGAC	4 cut(s)	561, 652, 904, 1443
BslI	CCNNNNNNNGG	8 cut(s)	183, 1292, 1555, 1910, 2004, 2066, 2134, 2474
BsmAI	GTCTC	5 cut(s)	117, 511, 557, 800, 958
BsmFI	GGGAC	4 cut(s)	561, 652, 904, 1443
BsnI	GGCC	5 cut(s)	175, 324, 459, 816, 1644
BsoBI	CYCGRG	1 cut(s)	404
Bsp1286I	GDGCHC	4 cut(s)	207, 1035, 1047, 1934
Bsp13I	TCCGGA	2 cut(s)	207, 2059
Bsp19I	CCATGG	4 cut(s)	144, 762, 1220, 1286
BspACI	CCGC	6 cut(s)	666, 1464, 1580, 1946, 2005, 2474
BspANI	GGCC	5 cut(s)	175, 324, 459, 816, 1644
BspCNI	CTCAG	4 cut(s)	954, 1383, 1822, 2395
BspEI	TCCGGA	2 cut(s)	207, 2059
BspFNI	CGCG	1 cut(s)	2474
BspLI	GGNNCC	6 cut(s)	150, 212, 640, 641, 1007, 1996
BspPI	GGATC	6 cut(s)	244, 1245, 1692, 2070, 2307, 2486
BspQI	GCTCTTC	3 cut(s)	1035, 1896, 2387
BspTI	CTTAAG	2 cut(s)	2489, 2555
BsrDI	GCAATG	1 cut(s)	203
BsrFI	RCCGGY	1 cut(s)	427
BsrI	ACTGG	5 cut(s)	649, 816, 1158, 1813, 2428
BssAI	RCCGGY	1 cut(s)	427
BssECI	CCNNGG	7 cut(s)	144, 184, 762, 989, 1220, 1286, 2443
BssNI	GRCGYC	1 cut(s)	1630
BssSI	CACGAG	1 cut(s)	1849
BssT1I	CCWWGG	6 cut(s)	144, 762, 989, 1220, 1286, 2443
Bst2BI	CACGAG	1 cut(s)	1849
Bst2UI	CCWGG	3 cut(s)	222, 313, 2129
Bst4CI	ACNGT	6 cut(s)	904, 982, 1472, 1495, 1558, 2124
Bst6I	CTCTTC	4 cut(s)	366, 1035, 1896, 2387
BstACI	GRCGYC	1 cut(s)	1630
BstAFI	CTTAAG	2 cut(s)	2489, 2555
BstAPI	GCANNNNNTGC	2 cut(s)	202, 916
BstC8I	GCNNGC	7 cut(s)	141, 194, 292, 461, 1227, 1527, 2259
BstDEI	CTNAG	6 cut(s)	962, 1211, 1370, 1830, 1898, 2403
BstDSI	CCRYGG	4 cut(s)	144, 762, 1220, 1286
BstENI	CCTNNNNNAGG	1 cut(s)	2132
BstF5I	GGATG	7 cut(s)	194, 645, 772, 1216, 1282, 1522, 1920
BstFNI	CGCG	1 cut(s)	2474
BstH2I	RGCGCY	1 cut(s)	494
BstHHI	GCGC	1 cut(s)	493
BstMAI	GTCTC	5 cut(s)	117, 511, 557, 800, 958
BstMWI	GCNNNNNNNGC	3 cut(s)	140, 202, 916
BstNI	CCWGG	3 cut(s)	222, 313, 2129
BstNSI	RCATGY	2 cut(s)	467, 2175
BstSCI	CCNGG	5 cut(s)	183, 220, 311, 1909, 2127
BstSLI	GKGCMC	2 cut(s)	1035, 1934
BstUI	CGCG	1 cut(s)	2474
BstV1I	GCAGC	4 cut(s)	383, 1326, 1849, 1898
BstV2I	GAAGAC	2 cut(s)	689, 777
BstX2I	RGATCY	6 cut(s)	504, 1093, 1684, 1753, 2062, 2478
BstXI	CCANNNNNNTGG	1 cut(s)	734
BstYI	RGATCY	6 cut(s)	504, 1093, 1684, 1753, 2062, 2478
BsuRI	GGCC	5 cut(s)	175, 324, 459, 816, 1644
BtgI	CCRYGG	4 cut(s)	144, 762, 1220, 1286
BtsCI	GGATG	7 cut(s)	194, 645, 772, 1216, 1282, 1522, 1920
BtsI	GCAGTG	2 cut(s)	129, 327
BtsIMutI	CAGTG	5 cut(s)	129, 327, 566, 809, 1468
Cac8I	GCNNGC	7 cut(s)	141, 194, 292, 461, 1227, 1527, 2259
CaiI	CAGNNNCTG	1 cut(s)	2128
CfoI	GCGC	1 cut(s)	493
Cfr10I	RCCGGY	1 cut(s)	427
Cfr13I	GGNCC	3 cut(s)	174, 639, 814
Csp6I	GTAC	8 cut(s)	261, 416, 556, 1806, 1834, 2337, 2418, 2541
CviQI	GTAC	8 cut(s)	261, 416, 556, 1806, 1834, 2337, 2418, 2541
DdeI	CTNAG	6 cut(s)	962, 1211, 1370, 1830, 1898, 2403
DraI	TTTAAA	1 cut(s)	1672
EaeI	YGGCCR	1 cut(s)	322
Eam1104I	CTCTTC	4 cut(s)	366, 1035, 1896, 2387
EarI	CTCTTC	4 cut(s)	366, 1035, 1896, 2387
Eco130I	CCWWGG	6 cut(s)	144, 762, 989, 1220, 1286, 2443
Eco147I	AGGCCT	2 cut(s)	459, 1644
Eco47I	GGWCC	1 cut(s)	639
Eco47III	AGCGCT	1 cut(s)	492
Eco88I	CYCGRG	1 cut(s)	404
EcoNI	CCTNNNNNAGG	1 cut(s)	2132
EcoO109I	RGGNCCY	1 cut(s)	639
EcoRI	GAATTC	1 cut(s)	715
EcoRII	CCWGG	3 cut(s)	220, 311, 2127
EcoT14I	CCWWGG	6 cut(s)	144, 762, 989, 1220, 1286, 2443
EcoT22I	ATGCAT	2 cut(s)	1262, 2236
ErhI	CCWWGG	6 cut(s)	144, 762, 989, 1220, 1286, 2443
FaqI	GGGAC	4 cut(s)	561, 652, 904, 1443
FauI	CCCGC	2 cut(s)	1998, 2467
FbaI	TGATCA	1 cut(s)	856
FblI	GTMKAC	2 cut(s)	248, 1191
Fnu4HI	GCNGC	5 cut(s)	397, 1340, 1863, 1887, 1946
FokI	GGATG	7 cut(s)	201, 632, 779, 1203, 1269, 1529, 1907
Fsp4HI	GCNGC	5 cut(s)	397, 1340, 1863, 1887, 1946
FspBI	CTAG	6 cut(s)	818, 926, 947, 1131, 1187, 2316
GlaI	GCGC	1 cut(s)	492
GluI	GCNGC	5 cut(s)	397, 1340, 1863, 1887, 1946
GsuI	CTGGAG	1 cut(s)	334
HaeII	RGCGCY	1 cut(s)	494
HaeIII	GGCC	5 cut(s)	175, 324, 459, 816, 1644
HapII	CCGG	8 cut(s)	184, 208, 428, 593, 799, 1107, 1911, 2060
HhaI	GCGC	1 cut(s)	493
Hin1I	GRCGYC	1 cut(s)	1630
Hin6I	GCGC	1 cut(s)	491
HinP1I	GCGC	1 cut(s)	491
HincII	GTYRAC	2 cut(s)	249, 439
HindII	GTYRAC	2 cut(s)	249, 439
HindIII	AAGCTT	2 cut(s)	585, 2535
HinfI	GANTC	9 cut(s)	31, 154, 245, 283, 697, 847, 941, 2078, 2240
HpaII	CCGG	8 cut(s)	184, 208, 428, 593, 799, 1107, 1911, 2060
HphI	GGTGA	5 cut(s)	298, 718, 916, 1491, 2155
Hpy166II	GTNNAC	8 cut(s)	249, 439, 880, 1033, 1192, 1738, 1904, 2177
Hpy188III	TCNNGA	8 cut(s)	158, 208, 404, 581, 844, 1751, 2060, 2389
Hpy8I	GTNNAC	8 cut(s)	249, 439, 880, 1033, 1192, 1738, 1904, 2177
HpyCH4III	ACNGT	6 cut(s)	904, 982, 1472, 1495, 1558, 2124
HpyCH4IV	ACGT	3 cut(s)	1055, 1630, 1734
HpyF10VI	GCNNNNNNNGC	3 cut(s)	140, 202, 916
HpyF3I	CTNAG	6 cut(s)	962, 1211, 1370, 1830, 1898, 2403
HpySE526I	ACGT	3 cut(s)	1055, 1630, 1734
Hsp92I	GRCGYC	1 cut(s)	1630
HspAI	GCGC	1 cut(s)	491
KflI	GGGWCCC	1 cut(s)	639
Kpn2I	TCCGGA	2 cut(s)	207, 2059
Ksp22I	TGATCA	1 cut(s)	856
LguI	GCTCTTC	3 cut(s)	1035, 1896, 2387
LmnI	GCTCC	2 cut(s)	210, 1800
Lsp1109I	GCAGC	4 cut(s)	383, 1326, 1849, 1898
LweI	GCATC	9 cut(s)	179, 561, 1097, 1117, 1264, 1321, 1496, 1567, 1771
MaeI	CTAG	6 cut(s)	818, 926, 947, 1131, 1187, 2316
MaeII	ACGT	3 cut(s)	1055, 1630, 1734
MaeIII	GTNAC	2 cut(s)	1436, 1552
MfeI	CAATTG	1 cut(s)	2082
MflI	RGATCY	6 cut(s)	504, 1093, 1684, 1753, 2062, 2478
MhlI	GDGCHC	4 cut(s)	207, 1035, 1047, 1934
MlsI	TGGCCA	1 cut(s)	324
MluNI	TGGCCA	1 cut(s)	324
MlyI	GAGTC	3 cut(s)	254, 277, 2234
MmeI	TCCRAC	2 cut(s)	1215, 2380
Mox20I	TGGCCA	1 cut(s)	324
Mph1103I	ATGCAT	2 cut(s)	1262, 2236
MroI	TCCGGA	2 cut(s)	207, 2059
MroXI	GAANNNNTTC	2 cut(s)	2123, 2377
MscI	TGGCCA	1 cut(s)	324
MseI	TTAA	9 cut(s)	27, 48, 795, 1200, 1671, 1784, 2291, 2490, 2556
MslI	CAYNNNNRTG	3 cut(s)	93, 363, 1703
Msp20I	TGGCCA	1 cut(s)	324
MspCI	CTTAAG	2 cut(s)	2489, 2555
MspI	CCGG	8 cut(s)	184, 208, 428, 593, 799, 1107, 1911, 2060
MspR9I	CCNGG	5 cut(s)	185, 222, 313, 1911, 2129
MunI	CAATTG	1 cut(s)	2082
MvaI	CCWGG	3 cut(s)	222, 313, 2129
MvnI	CGCG	1 cut(s)	2474
MwoI	GCNNNNNNNGC	3 cut(s)	140, 202, 916
NciI	CCSGG	2 cut(s)	185, 1911
NcoI	CCATGG	4 cut(s)	144, 762, 1220, 1286
NlaIV	GGNNCC	6 cut(s)	150, 212, 640, 641, 1007, 1996
NmuCI	GTSAC	1 cut(s)	1436
NsiI	ATGCAT	2 cut(s)	1262, 2236
NspI	RCATGY	2 cut(s)	467, 2175
PceI	AGGCCT	2 cut(s)	459, 1644
PciSI	GCTCTTC	3 cut(s)	1035, 1896, 2387
PdmI	GAANNNNTTC	2 cut(s)	2123, 2377
PfeI	GAWTC	6 cut(s)	31, 154, 697, 847, 941, 2078
PfoI	TCCNGGA	1 cut(s)	2127
PkrI	GCNGC	5 cut(s)	398, 1341, 1864, 1888, 1947
PleI	GAGTC	3 cut(s)	253, 277, 2234
PpsI	GAGTC	3 cut(s)	253, 277, 2234
PpuMI	RGGWCCY	1 cut(s)	639
PsiI	TTATAA	1 cut(s)	603
Psp1406I	AACGTT	1 cut(s)	1055
Psp5II	RGGWCCY	1 cut(s)	639
Psp6I	CCWGG	3 cut(s)	220, 311, 2127
PspGI	CCWGG	3 cut(s)	220, 311, 2127
PspN4I	GGNNCC	6 cut(s)	150, 212, 640, 641, 1007, 1996
PspPI	GGNCC	3 cut(s)	174, 639, 814
PspPPI	RGGWCCY	1 cut(s)	639
PsrI	GAACNNNNNNTAC	2 cut(s)	2404, 2436
PstNI	CAGNNNCTG	1 cut(s)	2128
PsuI	RGATCY	6 cut(s)	504, 1093, 1684, 1753, 2062, 2478
RsaI	GTAC	8 cut(s)	262, 417, 557, 1807, 1835, 2338, 2419, 2542
RsaNI	GTAC	8 cut(s)	261, 416, 556, 1806, 1834, 2337, 2418, 2541
RseI	CAYNNNNRTG	3 cut(s)	93, 363, 1703
SalI	GTCGAC	1 cut(s)	247
SapI	GCTCTTC	3 cut(s)	1035, 1896, 2387
SaqAI	TTAA	9 cut(s)	27, 48, 795, 1200, 1671, 1784, 2291, 2490, 2556
SatI	GCNGC	5 cut(s)	397, 1340, 1863, 1887, 1946
Sau96I	GGNCC	3 cut(s)	174, 639, 814
ScaI	AGTACT	2 cut(s)	557, 1807
SchI	GAGTC	3 cut(s)	254, 277, 2234
ScrFI	CCNGG	5 cut(s)	185, 222, 313, 1911, 2129
SduI	GDGCHC	4 cut(s)	207, 1035, 1047, 1934
SfaNI	GCATC	9 cut(s)	179, 561, 1097, 1117, 1264, 1321, 1496, 1567, 1771
SinI	GGWCC	1 cut(s)	639
SmiMI	CAYNNNNRTG	3 cut(s)	93, 363, 1703
SmlI	CTYRAG	4 cut(s)	1679, 1925, 2489, 2555
SmoI	CTYRAG	4 cut(s)	1679, 1925, 2489, 2555
SseBI	AGGCCT	2 cut(s)	459, 1644
SsiI	CCGC	6 cut(s)	666, 1464, 1580, 1946, 2005, 2474
SspI	AATATT	1 cut(s)	1383
SspMI	CTAG	6 cut(s)	818, 926, 947, 1131, 1187, 2316
StuI	AGGCCT	2 cut(s)	459, 1644
StyD4I	CCNGG	5 cut(s)	183, 220, 311, 1909, 2127
StyI	CCWWGG	6 cut(s)	144, 762, 989, 1220, 1286, 2443
TaaI	ACNGT	6 cut(s)	904, 982, 1472, 1495, 1558, 2124
TaiI	ACGT	3 cut(s)	1058, 1633, 1737
TaqI	TCGA	5 cut(s)	248, 281, 1325, 1692, 2390
TatI	WGTACW	5 cut(s)	415, 555, 1805, 2417, 2540
TauI	GCSGC	1 cut(s)	1948
TfiI	GAWTC	6 cut(s)	31, 154, 697, 847, 941, 2078
Tru1I	TTAA	9 cut(s)	27, 48, 795, 1200, 1671, 1784, 2291, 2490, 2556
Tru9I	TTAA	9 cut(s)	27, 48, 795, 1200, 1671, 1784, 2291, 2490, 2556
TscAI	CASTG	5 cut(s)	136, 334, 573, 816, 1475
TseFI	GTSAC	1 cut(s)	1436
TseI	GCWGC	4 cut(s)	396, 1339, 1862, 1886
Tsp45I	GTSAC	1 cut(s)	1436
TspRI	CASTG	5 cut(s)	136, 334, 573, 816, 1475
Vha464I	CTTAAG	2 cut(s)	2489, 2555
VneI	GTGCAC	1 cut(s)	1031
VpaK11BI	GGWCC	1 cut(s)	639
XagI	CCTNNNNNAGG	1 cut(s)	2132
XapI	RAATTY	4 cut(s)	709, 715, 1171, 1954
XceI	RCATGY	2 cut(s)	467, 2175
XcmI	CCANNNNNNNNNTGG	2 cut(s)	228, 2330
XmiI	GTMKAC	2 cut(s)	248, 1191
XmnI	GAANNNNTTC	2 cut(s)	2123, 2377
XspI	CTAG	6 cut(s)	818, 926, 947, 1131, 1187, 2316
ZraI	GACGTC	1 cut(s)	1631
ZrmI	AGTACT	2 cut(s)	557, 1807
Zsp2I	ATGCAT	2 cut(s)	1262, 2236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.