Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0006120

Physical Location & Seq

Genomic Coordinates Forward (+)

72367951 .. 72373838

Gene Structure 5888 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG545800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2568 bp

ATGGAATTGCCAGACTACTCATTGGTTAAGAATCTTGTAATGAAGGTTAAAAAGGAGATGTTCTCATCTTCATCTTCATCATCATCATCAGAACATGATGATGTATATCGTCTTGTGTCTCCTTCTGCTTACGACACTGCTTGGCTCGCCATGGTTCCGAATCCTGAAAAACCAGATCGGCCCTTGTTCCGGGGATGCTTGCAATGGGTGCTCCGGAACCAAAAACCAGGAGGCTTCTGGGGATCATCAGAGTCGACCTCTATAAGTACCTTATACTCCCTCACTTCGACTCTCGCTTGCATTGTTGCACTCACCACCTGGAGTGTTGGCCACACTGCCATTCAAAAGGGGTTGGGGTTCATTCATGCAAATGCAGAAGAGATACTGAAAGAGCAAAATGGCTGCTTTCCCGAGTGGTTTGTACTTCTTTTACCGGCAATAGTTGACCTTGCAAAGACCAAAGGCCTGCATGTTCATTTCTCAAATGGTTCAAAAGCGCTTATACAACAGATCTTCCATGAGAGACAACAACTATTCCAAATCAACAGGTCGGGACAGGAGTACTATGAGACACTGATGCCATATCTTGAAGCTTTGCCGGAGAGTTATAATGAGATTTTGCTACAACAGAGTGAAGATTATGGGATCCTCATCCAGTTTCCATCAGCCACCGCCTATGCTTTTATGAAGACAGGAAATAAGAATCTTTTGGTGAATTTGAATTCTGTTGTCCAAAGATGTGGATATGGAGTTCCTGCGATTTACCCCATGGACGAAGACCTCGTAAAGATTTGTCTTATTAACCGGATTGAGACACTGGGCCTAGCAGAGTATTTCATGGAGGAGATAACGATTCTACTTGATCAAGTTTTCAGAAGTTATGTGAACAATGAAGGGACAGAGATGACGGTGAAGCATGGTCTGCTTCCACTAGAACTTTATAGAGATTCACTAGCATTTCGGCTACTGAGACTACATGGCTATACTGTATCTCCTTGGAAGTTCTGCTGGTTCCTCCAAGATGAAGATATATTGATGCACATAGAAGAGCACCATGAAACGTTTCTGAGTGCCATGTATAATGTATTCAGGGCATCAGATCTTACTTTTGCCGGAGAAAGTCAGCTTGAAGATGCTAGGTTATTCTCTACAAGAATACTGGAAAAGGAAACAACAAATTCCGAGAAATTGCTAGTAGACCAGATTAAGCATGAACTTAGTCATCCATGGCTTGCTCGGTTGGATCATTTGGAACACAGGAAATGCATTGAGAGGGAAGATGCTATTCATCCATGGACAGGAAAAGCCTTTTCGTATAGACTATCATTTTCGAGTGATGCTCTGCTGCAACAACTTGCCATAGAGAATTATTCACTCAGACAATCAATATTCAGAGACGAACTAACCGAGTTAGAGAAGTGGTCAAAGGACATGGGACTTTGTGACCTGGGGTTTGCACGACAGAAAACCGCACACTGTTATTTTGCAGTTGCATCAACTGTATCACCTCCTTCCCTTTCGGATGTGAGGCTGGCACTAGTCAAAAGTGCAATCCTTGTTACGGTGGCTGATGATTTTTTTGATGCGGAAGGTTCCTTGAGTGATCTCAAAAGTTTTACAAATGCCGTCAAAAGACGTCAGCAGATAGGCCTGAATGGACACAGCAAAACTATATTTAAAGCTCTCAAGGATCTCCTCGATGACATTTCTATGCAATTCTTCAATAAATATGGATATGACGTAAACATATACCTTCAAGATCTATGGCATCAAACATTTGTGAAATGGTTAAAGGAGGCAGAGTGGAGCAGTACTGGGCATACCCCATCAATAGCTGAGTACCTTCAAGTAGCCACGAGTTCAATAGCTGCACAAACTATTATTCTTCCAGCAGCTCTTCTTCTTAGTCCACAACCCCGAATAGACATCCTCAAGTGCCCTCAAAATGAGCCGCTTACAAATTTACTTATGGTTTCAACACGCTTTTTGAACGATATTGGAACCTATAAGCGGGAACAAGAGGAGGGGAAGCCAAACCTTGTTCTGCTACACATGAAGGAAAATCCGGATCTGGGAGTTGAAGAATCAATTGAGGTTATACAGAAGATATTGGACGAAAAGAAGAAACAGTTCCTGGAACAGGGTTTGGTGAATGGAGTGAATGATATGCCAGAAGCATGTAAACTACTCCACTTTCAATGCCTGAAAGCATTTCAGATGTTCTACAACTCAACAAATGCATTTGACTCCCAAACAGAGTTGCTTGCTGACATCAACAAAGCGATTTATTTTCCATTAAAGGTGGATCATAGAAGTTTGGCTAGTAGCCATAGCCAAGTTGGTACTAAAAGCCTTCAAATCCAACAGAGAGTAATTGGAACTAGTTCAACATTCTCGAAGAGCATTACTGAGAACAGAACTTGTACTACTGGTGCTATAAAGTTCCTCCAAGGAAAGAAGTTTTCTGCAAAGCCTTACGCGGGGATCTTATCACTTAAGATGAGAAAATCAATAACTCCTACGACTTTCCCAAAGCCGAAAGCTTGTACTTATTCGTGGCATAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0000304 GO:0001101 GO:0003674 GO:0003824 GO:0006629 GO:0006720 GO:0006721 GO:0006950 GO:0006979 GO:0007584 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0016101 GO:0016102 GO:0016114 GO:0016829 GO:0016835 GO:0016838 GO:0031667 GO:0042221 GO:0042493 GO:0043207 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0080013 GO:0080027 GO:1901576 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00904 map00904

Pfam Domains

Protein Families

Terpene_synth Terpene_synth_C

Protein Analysis

855

Amino Acids

97.26

Weight (kDa)

6.23

Isoelectric Point (pI)

46.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Terpene_synth	PF01397	221 - 406	5.8e-31	Terpene synthase, N-terminal domain
Terpene_synth_C	PF03936	476 - 709	4.4e-53	Terpene synthase family, metal binding domain
Terpene_syn_C_2	PF19086	516 - 709	1.2e-35	Terpene synthase family 2, C-terminal metal binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006120)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61120
malus_domestica	MD17G1080300.v1.1 MD17G1080400.v1.1 MD17G1080500.v1.1 MD17G1080700.v1.1 MD17G1080800.v1.1
prunus_persica	Prupe.3G234300_v2.0.a1 Prupe.3G234400_v2.0.a1 Prupe.3G234400_v2.0.a1 Prupe.3G234500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0162311
rosa_laevigata	RLG00000021334 RLG00000021337
rosa_multiflora	Rmu_co8462281.1_g000001 Rmu_sc0004020.1_g000013
rosa_roxburghii	Rroxscaffold_2G00088040 Rroxscaffold_2G00088060 Rroxscaffold_2G00088110
rosa_rugosa	Rorug02G0496200 Rorug02G0496300
rosa_samantha	Rh2AG562300 Rh2AG562400 Rh2BG575400 Rh2CG545800 Rh2DG585200 Rh2DG585600
rosa_wichuraiana	Rw2G046570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	609
AatII	GACGTC	1 cut(s)	1639
AccI	GTMKAC	2 cut(s)	254, 1197
AccII	CGCG	1 cut(s)	2480
AccIII	TCCGGA	2 cut(s)	213, 2065
AciI	CCGC	6 cut(s)	672, 1470, 1586, 1952, 2011, 2480
AclI	AACGTT	1 cut(s)	1061
AclWI	GGATC	8 cut(s)	250, 640, 653, 1251, 1698, 2076, 2313, 2492
AcoI	YGGCCR	1 cut(s)	328
AcsI	RAATTY	4 cut(s)	715, 721, 1177, 1960
AcyI	GRCGYC	1 cut(s)	1636
AfaI	GTAC	8 cut(s)	268, 423, 563, 1813, 1841, 2344, 2425, 2548
AfeI	AGCGCT	1 cut(s)	498
AfiI	CCNNNNNNNGG	7 cut(s)	189, 1298, 1561, 2010, 2072, 2140, 2480
AflII	CTTAAG	1 cut(s)	2495
AhlI	ACTAGT	2 cut(s)	1537, 2381
AjnI	CCWGG	4 cut(s)	226, 317, 1446, 2133
AloI	GAACNNNNNNTCC	2 cut(s)	735, 767
AluBI	AGCT	7 cut(s)	593, 1126, 1682, 1835, 1868, 1895, 2543
AluI	AGCT	7 cut(s)	593, 1126, 1682, 1835, 1868, 1895, 2543
Alw21I	GWGCWC	2 cut(s)	213, 1053
Alw26I	GTCTC	6 cut(s)	123, 517, 563, 806, 964, 1389
AlwI	GGATC	8 cut(s)	250, 640, 653, 1251, 1698, 2076, 2313, 2492
AlwNI	CAGNNNCTG	1 cut(s)	2134
Ama87I	CYCGRG	1 cut(s)	410
Aor13HI	TCCGGA	2 cut(s)	213, 2065
Aor51HI	AGCGCT	1 cut(s)	498
AoxI	GGCC	5 cut(s)	179, 328, 463, 820, 1648
ApeKI	GCWGC	4 cut(s)	402, 1345, 1868, 1892
ApoI	RAATTY	4 cut(s)	715, 721, 1177, 1960
ArsI	GACNNNNNNTTYG	2 cut(s)	1436, 1468
Asp700I	GAANNNNTTC	3 cut(s)	1062, 2129, 2383
AspLEI	GCGC	1 cut(s)	499
AspS9I	GGNCC	2 cut(s)	180, 820
AsuC2I	CCSGG	1 cut(s)	191
AsuHPI	GGTGA	5 cut(s)	304, 724, 922, 1497, 2161
AvaI	CYCGRG	1 cut(s)	410
BaeGI	GKGCMC	1 cut(s)	1940
BalI	TGGCCA	1 cut(s)	330
BamHI	GGATCC	1 cut(s)	645
BauI	CACGAG	1 cut(s)	1855
BbsI	GAAGAC	2 cut(s)	695, 783
Bbv12I	GWGCWC	2 cut(s)	213, 1053
BbvI	GCAGC	4 cut(s)	389, 1332, 1855, 1904
BccI	CCATC	2 cut(s)	670, 1834
BceAI	ACGGC	1 cut(s)	1610
BciT130I	CCWGG	4 cut(s)	228, 319, 1448, 2135
BclI	TGATCA	1 cut(s)	862
BcnI	CCSGG	1 cut(s)	191
BcoDI	GTCTC	6 cut(s)	123, 517, 563, 806, 964, 1389
BcuI	ACTAGT	2 cut(s)	1537, 2381
BfaI	CTAG	8 cut(s)	824, 932, 953, 1137, 1193, 1538, 2322, 2382
BfoI	RGCGCY	1 cut(s)	500
BfrI	CTTAAG	1 cut(s)	2495
BglII	AGATCT	3 cut(s)	510, 1099, 1759
BisI	GCNGC	5 cut(s)	403, 1346, 1869, 1893, 1952
BlsI	GCNGC	5 cut(s)	404, 1347, 1870, 1894, 1953
BmcAI	AGTACT	2 cut(s)	563, 1813
Bme1390I	CCNGG	5 cut(s)	191, 228, 319, 1448, 2135
BmeT110I	CYCGRG	1 cut(s)	410
BmgT120I	GGNCC	2 cut(s)	180, 820
BmiI	GGNNCC	6 cut(s)	156, 218, 647, 1013, 1594, 2002
BmrFI	CCNGG	5 cut(s)	191, 228, 319, 1448, 2135
BmrI	ACTGGG	2 cut(s)	827, 1824
BmuI	ACTGGG	2 cut(s)	827, 1824
BpiI	GAAGAC	2 cut(s)	695, 783
BplI	GAGNNNNNCTC	6 cut(s)	47, 79, 242, 274, 1324, 1356
BpmI	CTGGAG	1 cut(s)	340
BpuEI	CTTGAG	3 cut(s)	1618, 1670, 1916
BpuMI	CCSGG	1 cut(s)	191
BsaBI	GATNNNNATC	2 cut(s)	105, 650
BsaHI	GRCGYC	1 cut(s)	1636
BsaJI	CCNNGG	8 cut(s)	150, 190, 768, 995, 1226, 1292, 1447, 2449
BsaWI	WCCGGW	3 cut(s)	213, 804, 2065
BsaXI	ACNNNNNCTCC	4 cut(s)	976, 1006, 1787, 1817
Bsc4I	CCNNNNNNNGG	7 cut(s)	189, 1298, 1561, 2010, 2072, 2140, 2480
Bse118I	RCCGGY	1 cut(s)	433
Bse1I	ACTGG	5 cut(s)	655, 822, 1164, 1819, 2434
Bse3DI	GCAATG	1 cut(s)	209
Bse8I	GATNNNNATC	2 cut(s)	105, 650
BseAI	TCCGGA	2 cut(s)	213, 2065
BseBI	CCWGG	4 cut(s)	228, 319, 1448, 2135
BseDI	CCNNGG	8 cut(s)	150, 190, 768, 995, 1226, 1292, 1447, 2449
BseGI	GGATG	6 cut(s)	200, 651, 1222, 1288, 1528, 1926
BseJI	GATNNNNATC	2 cut(s)	105, 650
BseLI	CCNNNNNNNGG	7 cut(s)	189, 1298, 1561, 2010, 2072, 2140, 2480
BseMI	GCAATG	1 cut(s)	209
BseMII	CTCAG	5 cut(s)	959, 1058, 1390, 1827, 2400
BseNI	ACTGG	5 cut(s)	655, 822, 1164, 1819, 2434
BseRI	GAGGAG	3 cut(s)	857, 1685, 2036
BseSI	GKGCMC	1 cut(s)	1940
BseXI	GCAGC	4 cut(s)	389, 1332, 1855, 1904
BsgI	GTGCAG	1 cut(s)	1854
Bsh1236I	CGCG	1 cut(s)	2480
BshFI	GGCC	5 cut(s)	181, 330, 465, 822, 1650
BsiHKAI	GWGCWC	2 cut(s)	213, 1053
BsiHKCI	CYCGRG	1 cut(s)	410
BsiSI	CCGG	7 cut(s)	190, 214, 434, 599, 805, 1113, 2066
BslFI	GGGAC	3 cut(s)	567, 910, 1449
BslI	CCNNNNNNNGG	7 cut(s)	189, 1298, 1561, 2010, 2072, 2140, 2480
BsmAI	GTCTC	6 cut(s)	123, 517, 563, 806, 964, 1389
BsmBI	CGTCTC	1 cut(s)	1389
BsmFI	GGGAC	3 cut(s)	567, 910, 1449
BsnI	GGCC	5 cut(s)	181, 330, 465, 822, 1650
BsoBI	CYCGRG	1 cut(s)	410
Bsp1286I	GDGCHC	3 cut(s)	213, 1053, 1940
Bsp13I	TCCGGA	2 cut(s)	213, 2065
Bsp19I	CCATGG	4 cut(s)	150, 768, 1226, 1292
BspACI	CCGC	6 cut(s)	672, 1470, 1586, 1952, 2011, 2480
BspANI	GGCC	5 cut(s)	181, 330, 465, 822, 1650
BspCNI	CTCAG	5 cut(s)	960, 1059, 1389, 1828, 2401
BspEI	TCCGGA	2 cut(s)	213, 2065
BspFNI	CGCG	1 cut(s)	2480
BspLI	GGNNCC	6 cut(s)	156, 218, 647, 1013, 1594, 2002
BspPI	GGATC	8 cut(s)	250, 640, 653, 1251, 1698, 2076, 2313, 2492
BspQI	GCTCTTC	3 cut(s)	1041, 1902, 2393
BspTI	CTTAAG	1 cut(s)	2495
BsrDI	GCAATG	1 cut(s)	209
BsrFI	RCCGGY	1 cut(s)	433
BsrI	ACTGG	5 cut(s)	655, 822, 1164, 1819, 2434
BssAI	RCCGGY	1 cut(s)	433
BssECI	CCNNGG	8 cut(s)	150, 190, 768, 995, 1226, 1292, 1447, 2449
BssNI	GRCGYC	1 cut(s)	1636
BssSI	CACGAG	1 cut(s)	1855
BssT1I	CCWWGG	6 cut(s)	150, 768, 995, 1226, 1292, 2449
Bst2BI	CACGAG	1 cut(s)	1855
Bst2UI	CCWGG	4 cut(s)	228, 319, 1448, 2135
Bst4CI	ACNGT	6 cut(s)	910, 988, 1478, 1501, 1564, 2130
Bst6I	CTCTTC	4 cut(s)	372, 1041, 1902, 2393
BstACI	GRCGYC	1 cut(s)	1636
BstAFI	CTTAAG	1 cut(s)	2495
BstAPI	GCANNNNNTGC	2 cut(s)	208, 922
BstC8I	GCNNGC	7 cut(s)	147, 200, 298, 467, 1233, 1533, 2265
BstDEI	CTNAG	7 cut(s)	968, 1067, 1217, 1376, 1836, 1904, 2409
BstDSI	CCRYGG	4 cut(s)	150, 768, 1226, 1292
BstENI	CCTNNNNNAGG	1 cut(s)	2138
BstF5I	GGATG	6 cut(s)	200, 651, 1222, 1288, 1528, 1926
BstFNI	CGCG	1 cut(s)	2480
BstH2I	RGCGCY	1 cut(s)	500
BstHHI	GCGC	1 cut(s)	499
BstMAI	GTCTC	6 cut(s)	123, 517, 563, 806, 964, 1389
BstMWI	GCNNNNNNNGC	3 cut(s)	146, 208, 922
BstNI	CCWGG	4 cut(s)	228, 319, 1448, 2135
BstNSI	RCATGY	2 cut(s)	473, 2181
BstSCI	CCNGG	5 cut(s)	189, 226, 317, 1446, 2133
BstSLI	GKGCMC	1 cut(s)	1940
BstUI	CGCG	1 cut(s)	2480
BstV1I	GCAGC	4 cut(s)	389, 1332, 1855, 1904
BstV2I	GAAGAC	2 cut(s)	695, 783
BstX2I	RGATCY	7 cut(s)	510, 645, 1099, 1690, 1759, 2068, 2484
BstXI	CCANNNNNNTGG	1 cut(s)	740
BstYI	RGATCY	7 cut(s)	510, 645, 1099, 1690, 1759, 2068, 2484
BsuRI	GGCC	5 cut(s)	181, 330, 465, 822, 1650
BtgI	CCRYGG	4 cut(s)	150, 768, 1226, 1292
BtsCI	GGATG	6 cut(s)	200, 651, 1222, 1288, 1528, 1926
BtsI	GCAGTG	2 cut(s)	135, 333
BtsIMutI	CAGTG	5 cut(s)	135, 333, 572, 815, 1474
Cac8I	GCNNGC	7 cut(s)	147, 200, 298, 467, 1233, 1533, 2265
CaiI	CAGNNNCTG	1 cut(s)	2134
CfoI	GCGC	1 cut(s)	499
Cfr10I	RCCGGY	1 cut(s)	433
Cfr13I	GGNCC	2 cut(s)	180, 820
Csp6I	GTAC	8 cut(s)	267, 422, 562, 1812, 1840, 2343, 2424, 2547
CviQI	GTAC	8 cut(s)	267, 422, 562, 1812, 1840, 2343, 2424, 2547
DdeI	CTNAG	7 cut(s)	968, 1067, 1217, 1376, 1836, 1904, 2409
DraI	TTTAAA	1 cut(s)	1678
EaeI	YGGCCR	1 cut(s)	328
Eam1104I	CTCTTC	4 cut(s)	372, 1041, 1902, 2393
EarI	CTCTTC	4 cut(s)	372, 1041, 1902, 2393
Eco130I	CCWWGG	6 cut(s)	150, 768, 995, 1226, 1292, 2449
Eco147I	AGGCCT	2 cut(s)	465, 1650
Eco47III	AGCGCT	1 cut(s)	498
Eco88I	CYCGRG	1 cut(s)	410
EcoNI	CCTNNNNNAGG	1 cut(s)	2138
EcoRI	GAATTC	1 cut(s)	721
EcoRII	CCWGG	4 cut(s)	226, 317, 1446, 2133
EcoT14I	CCWWGG	6 cut(s)	150, 768, 995, 1226, 1292, 2449
EcoT22I	ATGCAT	2 cut(s)	1268, 2242
ErhI	CCWWGG	6 cut(s)	150, 768, 995, 1226, 1292, 2449
Esp3I	CGTCTC	1 cut(s)	1389
FaqI	GGGAC	3 cut(s)	567, 910, 1449
FauI	CCCGC	2 cut(s)	2004, 2473
FbaI	TGATCA	1 cut(s)	862
FblI	GTMKAC	2 cut(s)	254, 1197
Fnu4HI	GCNGC	5 cut(s)	403, 1346, 1869, 1893, 1952
FokI	GGATG	6 cut(s)	207, 638, 1209, 1275, 1535, 1913
Fsp4HI	GCNGC	5 cut(s)	403, 1346, 1869, 1893, 1952
FspBI	CTAG	8 cut(s)	824, 932, 953, 1137, 1193, 1538, 2322, 2382
GlaI	GCGC	1 cut(s)	498
GluI	GCNGC	5 cut(s)	403, 1346, 1869, 1893, 1952
GsuI	CTGGAG	1 cut(s)	340
HaeII	RGCGCY	1 cut(s)	500
HaeIII	GGCC	5 cut(s)	181, 330, 465, 822, 1650
HapII	CCGG	7 cut(s)	190, 214, 434, 599, 805, 1113, 2066
HhaI	GCGC	1 cut(s)	499
Hin1I	GRCGYC	1 cut(s)	1636
Hin6I	GCGC	1 cut(s)	497
HinP1I	GCGC	1 cut(s)	497
HincII	GTYRAC	2 cut(s)	255, 445
HindII	GTYRAC	2 cut(s)	255, 445
HindIII	AAGCTT	2 cut(s)	591, 2541
HinfI	GANTC	9 cut(s)	31, 160, 251, 289, 703, 853, 947, 2084, 2246
HpaII	CCGG	7 cut(s)	190, 214, 434, 599, 805, 1113, 2066
HphI	GGTGA	5 cut(s)	304, 724, 922, 1497, 2161
Hpy166II	GTNNAC	7 cut(s)	255, 445, 886, 1198, 1744, 1910, 2183
Hpy188III	TCNNGA	8 cut(s)	164, 214, 410, 552, 587, 1757, 2066, 2395
Hpy8I	GTNNAC	7 cut(s)	255, 445, 886, 1198, 1744, 1910, 2183
HpyAV	CCTTC	9 cut(s)	37, 132, 887, 1521, 1583, 1763, 1853, 2050, 2363
HpyCH4III	ACNGT	6 cut(s)	910, 988, 1478, 1501, 1564, 2130
HpyCH4IV	ACGT	3 cut(s)	1061, 1636, 1740
HpyF10VI	GCNNNNNNNGC	3 cut(s)	146, 208, 922
HpyF3I	CTNAG	7 cut(s)	968, 1067, 1217, 1376, 1836, 1904, 2409
HpySE526I	ACGT	3 cut(s)	1061, 1636, 1740
Hsp92I	GRCGYC	1 cut(s)	1636
HspAI	GCGC	1 cut(s)	497
Kpn2I	TCCGGA	2 cut(s)	213, 2065
Ksp22I	TGATCA	1 cut(s)	862
LguI	GCTCTTC	3 cut(s)	1041, 1902, 2393
LmnI	GCTCC	2 cut(s)	216, 1806
Lsp1109I	GCAGC	4 cut(s)	389, 1332, 1855, 1904
MaeI	CTAG	8 cut(s)	824, 932, 953, 1137, 1193, 1538, 2322, 2382
MaeII	ACGT	3 cut(s)	1061, 1636, 1740
MaeIII	GTNAC	2 cut(s)	1442, 1558
MfeI	CAATTG	1 cut(s)	2088
MflI	RGATCY	7 cut(s)	510, 645, 1099, 1690, 1759, 2068, 2484
MhlI	GDGCHC	3 cut(s)	213, 1053, 1940
MlsI	TGGCCA	1 cut(s)	330
MluNI	TGGCCA	1 cut(s)	330
MlyI	GAGTC	3 cut(s)	260, 283, 2240
MmeI	TCCRAC	2 cut(s)	1221, 2386
Mox20I	TGGCCA	1 cut(s)	330
Mph1103I	ATGCAT	2 cut(s)	1268, 2242
MroI	TCCGGA	2 cut(s)	213, 2065
MroXI	GAANNNNTTC	3 cut(s)	1062, 2129, 2383
MscI	TGGCCA	1 cut(s)	330
MseI	TTAA	8 cut(s)	27, 48, 801, 1206, 1677, 1790, 2297, 2496
MslI	CAYNNNNRTG	3 cut(s)	99, 369, 1709
Msp20I	TGGCCA	1 cut(s)	330
MspCI	CTTAAG	1 cut(s)	2495
MspI	CCGG	7 cut(s)	190, 214, 434, 599, 805, 1113, 2066
MspR9I	CCNGG	5 cut(s)	191, 228, 319, 1448, 2135
MunI	CAATTG	1 cut(s)	2088
MvaI	CCWGG	4 cut(s)	228, 319, 1448, 2135
MvnI	CGCG	1 cut(s)	2480
MwoI	GCNNNNNNNGC	3 cut(s)	146, 208, 922
NciI	CCSGG	1 cut(s)	191
NcoI	CCATGG	4 cut(s)	150, 768, 1226, 1292
NlaIV	GGNNCC	6 cut(s)	156, 218, 647, 1013, 1594, 2002
NmuCI	GTSAC	1 cut(s)	1442
NsiI	ATGCAT	2 cut(s)	1268, 2242
NspI	RCATGY	2 cut(s)	473, 2181
PceI	AGGCCT	2 cut(s)	465, 1650
PciSI	GCTCTTC	3 cut(s)	1041, 1902, 2393
PcsI	WCGNNNNNNNCGW	2 cut(s)	780, 1404
PdmI	GAANNNNTTC	3 cut(s)	1062, 2129, 2383
PfeI	GAWTC	6 cut(s)	31, 160, 703, 853, 947, 2084
PfoI	TCCNGGA	1 cut(s)	2133
PkrI	GCNGC	5 cut(s)	404, 1347, 1870, 1894, 1953
PleI	GAGTC	3 cut(s)	259, 283, 2240
PpsI	GAGTC	3 cut(s)	259, 283, 2240
PsiI	TTATAA	1 cut(s)	609
Psp1406I	AACGTT	1 cut(s)	1061
Psp6I	CCWGG	4 cut(s)	226, 317, 1446, 2133
PspGI	CCWGG	4 cut(s)	226, 317, 1446, 2133
PspN4I	GGNNCC	6 cut(s)	156, 218, 647, 1013, 1594, 2002
PspPI	GGNCC	2 cut(s)	180, 820
PsrI	GAACNNNNNNTAC	2 cut(s)	2410, 2442
PstNI	CAGNNNCTG	1 cut(s)	2134
PsuI	RGATCY	7 cut(s)	510, 645, 1099, 1690, 1759, 2068, 2484
RsaI	GTAC	8 cut(s)	268, 423, 563, 1813, 1841, 2344, 2425, 2548
RsaNI	GTAC	8 cut(s)	267, 422, 562, 1812, 1840, 2343, 2424, 2547
RseI	CAYNNNNRTG	3 cut(s)	99, 369, 1709
SalI	GTCGAC	1 cut(s)	253
SapI	GCTCTTC	3 cut(s)	1041, 1902, 2393
SaqAI	TTAA	8 cut(s)	27, 48, 801, 1206, 1677, 1790, 2297, 2496
SatI	GCNGC	5 cut(s)	403, 1346, 1869, 1893, 1952
Sau96I	GGNCC	2 cut(s)	180, 820
ScaI	AGTACT	2 cut(s)	563, 1813
SchI	GAGTC	3 cut(s)	260, 283, 2240
ScrFI	CCNGG	5 cut(s)	191, 228, 319, 1448, 2135
SduI	GDGCHC	3 cut(s)	213, 1053, 1940
SmiMI	CAYNNNNRTG	3 cut(s)	99, 369, 1709
SmlI	CTYRAG	4 cut(s)	1597, 1685, 1931, 2495
SmoI	CTYRAG	4 cut(s)	1597, 1685, 1931, 2495
SpeI	ACTAGT	2 cut(s)	1537, 2381
SseBI	AGGCCT	2 cut(s)	465, 1650
SsiI	CCGC	6 cut(s)	672, 1470, 1586, 1952, 2011, 2480
SspI	AATATT	1 cut(s)	1389
SspMI	CTAG	8 cut(s)	824, 932, 953, 1137, 1193, 1538, 2322, 2382
StuI	AGGCCT	2 cut(s)	465, 1650
StyD4I	CCNGG	5 cut(s)	189, 226, 317, 1446, 2133
StyI	CCWWGG	6 cut(s)	150, 768, 995, 1226, 1292, 2449
TaaI	ACNGT	6 cut(s)	910, 988, 1478, 1501, 1564, 2130
TaiI	ACGT	3 cut(s)	1064, 1639, 1743
TaqI	TCGA	5 cut(s)	254, 287, 1331, 1698, 2396
TatI	WGTACW	5 cut(s)	421, 561, 1811, 2423, 2546
TauI	GCSGC	1 cut(s)	1954
TfiI	GAWTC	6 cut(s)	31, 160, 703, 853, 947, 2084
Tru1I	TTAA	8 cut(s)	27, 48, 801, 1206, 1677, 1790, 2297, 2496
Tru9I	TTAA	8 cut(s)	27, 48, 801, 1206, 1677, 1790, 2297, 2496
TscAI	CASTG	5 cut(s)	142, 340, 579, 822, 1481
TseFI	GTSAC	1 cut(s)	1442
TseI	GCWGC	4 cut(s)	402, 1345, 1868, 1892
Tsp45I	GTSAC	1 cut(s)	1442
TspRI	CASTG	5 cut(s)	142, 340, 579, 822, 1481
Vha464I	CTTAAG	1 cut(s)	2495
XagI	CCTNNNNNAGG	1 cut(s)	2138
XapI	RAATTY	4 cut(s)	715, 721, 1177, 1960
XceI	RCATGY	2 cut(s)	473, 2181
XcmI	CCANNNNNNNNNTGG	2 cut(s)	234, 2336
XmiI	GTMKAC	2 cut(s)	254, 1197
XmnI	GAANNNNTTC	3 cut(s)	1062, 2129, 2383
XspI	CTAG	8 cut(s)	824, 932, 953, 1137, 1193, 1538, 2322, 2382
ZraI	GACGTC	1 cut(s)	1637
ZrmI	AGTACT	2 cut(s)	563, 1813
Zsp2I	ATGCAT	2 cut(s)	1268, 2242

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.