Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0006120

Physical Location & Seq

Genomic Coordinates Reverse (-)

10139040 .. 10144687

Gene Structure 5648 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00088110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2409 bp

ATGGAATTGCCAGACTACTCATTGGTTAAGACTCTTGTAATGAAGGTTAAAAAGGAGATGTTCTCATCTTCATCTTCATCATCAGAACATGATGATGATCATGTAGTGGATGATGTATATCGTCTTGTGTCTCCTTCTGCTTACGACACTGCTTGGCTCGCCATGGTTCCAAATCCTGAAAAACCAGATCGGCCCTTGTTCCGGGGATGCTTGCAATGGGTGCTCCGGAACCAAAAACCAGGAGGCTTCTGGGGATCATCAGAGTCGACCTCTATAAGTACCTTATACTCCCTCACTTCGACTCTCGCTTGCATTGTTGCACTCACCACCTGGAGTGTTGGCCACACTGCCATTCAAAAGGGGTTGGGGTTCATTCATGCAAATGCAGAAGAGATACTGAGAGAGCAAAATGGCTGCTTTCCCGAGTGGTTTGTACTTCTTTTACCGGCAATAGTTGACCTTGCAAAGACCAAAGGCCTGCATGTTCATTTCTCAAATGGTTCAAAAGAGCTTATACAACAGATATTCCATGAGAGACAACAACTATTCCAAATCAACAGGTCGGGACAGGATTACTATGAGACACTGATGCCATATCTTGAAGCTTTGCCGGAGAGTTATAATGAGATTTTGCTACAACAGAGTGAAGATTATGGGATCCTCATCCAGTTTCCATCAGCCACCGCCTATGCTTTTATGAAGACAGGAAATAAGAATCTTTTGGTGAATTTGAATTCTGTTGTCCAAAGATGTGGATATGGAGGGACAGAGATGACGGTGAAGCATGGTCTGCTTCCACTAGAACTTTATAGAGATTCACTAGCATTTCGGCTACTGAGACTACATGGCTATACTGTATCTCCTTGGAAGTTCTGCTGGTTCCTCCAAGATGAAGATATATTGGTGCACATAGAAGAGCACCATGAAACGTTTTTGAGTGCCATGTATAATGTATTCAGGGCATCAGATCTTACTTTTGCCGGAGAAAGTCAGCTTGAAGATGCTAGGTTATTCTCTACAAGAATACTGGAAAAGGAAACAACAAATTCCGAGAAATTGCTAGTAGACCAGATTAAGCATGAACTTAGTCATCCATGGCTTGCTCGGTTGGATCATTTGGAACACAGGAAATGCATTGAGAGGGAAGATGCTATTCATCCATGGACAGGAAAAGCCTTTTCGTATAGACTATCATTTTCGAGTGATGCTATGCTGCAACAACTTGCCATGGAGAATTATTCACTCAGACAATCAGTATTCAGAGATGAACTAACCGAGTTACAGATGTGGTCAAAGGACATGGGACTTTGTGACATGGGGTTTGCACGACAGAAAACCGCATACTGTTATTTTGCAGTTGCATCAACTGTATCATCTCCTTCCCTTTCGGATGTGAGACTGGCACTTGTCAAAAGTGCAATCCTTGTTACGGTGGCTGATGATTTTTTTGATGCGGAAGGTTCATTGAGTGATCTCAAAAGTCTTACCAATGCCGTCAAAAGACGGCAGCGGAAAGGCCTGAATGGATACAGCAAAACTATATTTAAAGCTCTCAAGGATCTCCTCGATGACATTTCAATGCAATTCTTCAATAAATATGGATGTGACTGGCATCAAACATTTGTGAAATGGTTAAAGGAGGCAGAGTGGAGCAGAACTGGGCATACCCCATCAATAGCTGAGTACCTTCAAGTCGCCACGAGCTCTATAGCTGCACAAACTATTACTCTTCCAGCAGCTCTTCTGCTTAGTCCACAAGGAATAGAATTCCTCAAGTGCCCTCAAAATGAGCCTCTTACAAATCTACTTATGGTTTCGACACGCCTTTTGAATGATATTGGAACCTACGAGCGGGAACAAGAGGAGGGGAAGCCAAACCTTGTTCTGCTACACATGAAGGAAAATCCTAATCTGGGAGTTGAAGAATCAATAGAGGTTATACAGAAGATATTGGATGAAACGAAGAAACAGTTTCTGGAACAGGTTTTGGTGAATGGAGTGAGTGATATGCCAGAAGCATGTAAACGTCTCCAATTTCAGTGCCTCAAAGCATTTCAGATGTTCTACAACTCAACAAATGCATTTGACTCCCAAACAGAGCTGCTTGCTGACATCAACAAAGCGATTTATTTTCCATTAAAGGTGGATCATAGAAGTTTGGCTAGTAGCCACAGCCAAGTTGGTACTAAAAGCCTTCAAATCCAACAGAGAGTAATTGGAACAAGTTCAACATTCTCGAAGAGCATTACTGAGAGCAGAACTAGTACTACTGGTGCTATAAAGTTCCTCCAAGGAAAGAAGTTTTCTGCAAAGCCTTACACGGGGATCTTATCACTTAAGATGAGAAAATCAATAACTCCAACGACTTGTCCAAAGCCGAAAGCTTGTACTTATTTGTGGCATAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0000304 GO:0001101 GO:0003674 GO:0003824 GO:0006629 GO:0006720 GO:0006721 GO:0006950 GO:0006979 GO:0007584 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0016101 GO:0016102 GO:0016114 GO:0016829 GO:0016835 GO:0016838 GO:0031667 GO:0042221 GO:0042493 GO:0043207 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0080013 GO:0080027 GO:1901576 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00904 map00904

Pfam Domains

Protein Families

Terpene_synth Terpene_synth_C

Protein Analysis

802

Amino Acids

91.12

Weight (kDa)

6.47

Isoelectric Point (pI)

43.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Terpene_synth	PF01397	268 - 362	3.1e-17	Terpene synthase, N-terminal domain
Terpene_synth_C	PF03936	432 - 656	7.3e-50	Terpene synthase family, metal binding domain
Terpene_syn_C_2	PF19086	472 - 656	2.7e-31	Terpene synthase family 2, C-terminal metal binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006120)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61120
malus_domestica	MD17G1080300.v1.1 MD17G1080400.v1.1 MD17G1080500.v1.1 MD17G1080700.v1.1 MD17G1080800.v1.1
prunus_persica	Prupe.3G234300_v2.0.a1 Prupe.3G234400_v2.0.a1 Prupe.3G234400_v2.0.a1 Prupe.3G234500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0162311
rosa_laevigata	RLG00000021334 RLG00000021337
rosa_multiflora	Rmu_co8462281.1_g000001 Rmu_sc0004020.1_g000013
rosa_roxburghii	Rroxscaffold_2G00088040 Rroxscaffold_2G00088060 Rroxscaffold_2G00088110
rosa_rugosa	Rorug02G0496200 Rorug02G0496300
rosa_samantha	Rh2AG562300 Rh2AG562400 Rh2BG575400 Rh2CG545800 Rh2DG585200 Rh2DG585600
rosa_wichuraiana	Rw2G046570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	621
AccBSI	CCGCTC	1 cut(s)	1852
AccI	GTMKAC	2 cut(s)	266, 1065
AccIII	TCCGGA	1 cut(s)	225
AciI	CCGC	5 cut(s)	684, 1338, 1454, 1510, 1852
AclI	AACGTT	1 cut(s)	929
AclWI	GGATC	7 cut(s)	262, 652, 665, 1119, 1566, 2154, 2333
AcoI	YGGCCR	1 cut(s)	340
AcsI	RAATTY	4 cut(s)	727, 733, 1045, 1766
AfaI	GTAC	6 cut(s)	280, 435, 1685, 2185, 2266, 2389
AfiI	CCNNNNNNNGG	6 cut(s)	201, 1166, 1429, 1851, 1913, 2321
AflII	CTTAAG	1 cut(s)	2336
AhlI	ACTAGT	1 cut(s)	2261
AjnI	CCWGG	2 cut(s)	238, 329
Alw21I	GWGCWC	4 cut(s)	225, 909, 921, 1706
Alw26I	GTCTC	6 cut(s)	135, 529, 575, 832, 1390, 2033
Alw44I	GTGCAC	1 cut(s)	905
AlwI	GGATC	7 cut(s)	262, 652, 665, 1119, 1566, 2154, 2333
AlwNI	CAGNNNCTG	1 cut(s)	1975
Ama87I	CYCGRG	1 cut(s)	422
Aor13HI	TCCGGA	1 cut(s)	225
AoxI	GGCC	4 cut(s)	191, 340, 475, 1516
ApaLI	GTGCAC	1 cut(s)	905
ApeKI	GCWGC	6 cut(s)	414, 1213, 1507, 1712, 1736, 2101
ApoI	RAATTY	4 cut(s)	727, 733, 1045, 1766
ArsI	GACNNNNNNTTYG	4 cut(s)	1304, 1336, 1810, 1842
Asp700I	GAANNNNTTC	1 cut(s)	2224
AspS9I	GGNCC	1 cut(s)	192
AsuC2I	CCSGG	1 cut(s)	203
AsuHPI	GGTGA	4 cut(s)	316, 736, 790, 2002
AvaI	CYCGRG	1 cut(s)	422
BaeGI	GKGCMC	2 cut(s)	909, 1781
BalI	TGGCCA	1 cut(s)	342
BamHI	GGATCC	1 cut(s)	657
BanII	GRGCYC	1 cut(s)	1706
BauI	CACGAG	1 cut(s)	1699
BbsI	GAAGAC	1 cut(s)	707
Bbv12I	GWGCWC	4 cut(s)	225, 909, 921, 1706
BbvI	GCAGC	6 cut(s)	401, 1200, 1519, 1699, 1748, 2088
BccI	CCATC	2 cut(s)	682, 1678
BceAI	ACGGC	2 cut(s)	1478, 1520
BciT130I	CCWGG	2 cut(s)	240, 331
BciVI	GTATCC	1 cut(s)	1520
BclI	TGATCA	1 cut(s)	97
BcnI	CCSGG	1 cut(s)	203
BcoDI	GTCTC	6 cut(s)	135, 529, 575, 832, 1390, 2033
BcuI	ACTAGT	1 cut(s)	2261
BfaI	CTAG	6 cut(s)	800, 821, 1005, 1061, 2163, 2262
BfmI	CTRYAG	1 cut(s)	1707
BfrI	CTTAAG	1 cut(s)	2336
BfuI	GTATCC	1 cut(s)	1520
BglII	AGATCT	1 cut(s)	967
BisI	GCNGC	6 cut(s)	415, 1214, 1508, 1713, 1737, 2102
BlsI	GCNGC	6 cut(s)	416, 1215, 1509, 1714, 1738, 2103
BmcAI	AGTACT	1 cut(s)	2266
Bme1390I	CCNGG	3 cut(s)	203, 240, 331
BmeT110I	CYCGRG	1 cut(s)	422
BmgT120I	GGNCC	1 cut(s)	192
BmiI	GGNNCC	5 cut(s)	168, 230, 659, 881, 1843
BmrFI	CCNGG	3 cut(s)	203, 240, 331
BmrI	ACTGGG	1 cut(s)	1668
BmsI	GCATC	9 cut(s)	197, 579, 971, 991, 1138, 1195, 1370, 1441, 1621
BmuI	ACTGGG	1 cut(s)	1668
BpiI	GAAGAC	1 cut(s)	707
BplI	GAGNNNNNCTC	4 cut(s)	47, 79, 254, 286
BpmI	CTGGAG	1 cut(s)	352
BpuEI	CTTGAG	2 cut(s)	1538, 1757
BpuMI	CCSGG	1 cut(s)	203
BsaBI	GATNNNNATC	3 cut(s)	96, 117, 662
BsaJI	CCNNGG	7 cut(s)	162, 202, 863, 1094, 1160, 1227, 2290
BsaWI	WCCGGW	1 cut(s)	225
BsaXI	ACNNNNNCTCC	4 cut(s)	844, 874, 1631, 1661
Bsc4I	CCNNNNNNNGG	6 cut(s)	201, 1166, 1429, 1851, 1913, 2321
Bse118I	RCCGGY	1 cut(s)	445
Bse1I	ACTGG	6 cut(s)	667, 1032, 1404, 1613, 1663, 2275
Bse3DI	GCAATG	1 cut(s)	221
Bse8I	GATNNNNATC	3 cut(s)	96, 117, 662
BseAI	TCCGGA	1 cut(s)	225
BseBI	CCWGG	2 cut(s)	240, 331
BseDI	CCNNGG	7 cut(s)	162, 202, 863, 1094, 1160, 1227, 2290
BseGI	GGATG	8 cut(s)	115, 212, 663, 1090, 1156, 1396, 1607, 1960
BseJI	GATNNNNATC	3 cut(s)	96, 117, 662
BseLI	CCNNNNNNNGG	6 cut(s)	201, 1166, 1429, 1851, 1913, 2321
BseMI	GCAATG	1 cut(s)	221
BseMII	CTCAG	5 cut(s)	389, 827, 1258, 1671, 2241
BseNI	ACTGG	6 cut(s)	667, 1032, 1404, 1613, 1663, 2275
BseRI	GAGGAG	2 cut(s)	1553, 1877
BseSI	GKGCMC	2 cut(s)	909, 1781
BseXI	GCAGC	6 cut(s)	401, 1200, 1519, 1699, 1748, 2088
BsgI	GTGCAG	1 cut(s)	1698
BshFI	GGCC	4 cut(s)	193, 342, 477, 1518
BsiHKAI	GWGCWC	4 cut(s)	225, 909, 921, 1706
BsiHKCI	CYCGRG	1 cut(s)	422
BsiSI	CCGG	5 cut(s)	202, 226, 446, 611, 981
BslFI	GGGAC	3 cut(s)	579, 778, 1317
BslI	CCNNNNNNNGG	6 cut(s)	201, 1166, 1429, 1851, 1913, 2321
BsmAI	GTCTC	6 cut(s)	135, 529, 575, 832, 1390, 2033
BsmBI	CGTCTC	1 cut(s)	2033
BsmFI	GGGAC	3 cut(s)	579, 778, 1317
BsnI	GGCC	4 cut(s)	193, 342, 477, 1518
BsoBI	CYCGRG	1 cut(s)	422
Bsp1286I	GDGCHC	5 cut(s)	225, 909, 921, 1706, 1781
Bsp13I	TCCGGA	1 cut(s)	225
Bsp19I	CCATGG	4 cut(s)	162, 1094, 1160, 1227
BspACI	CCGC	5 cut(s)	684, 1338, 1454, 1510, 1852
BspANI	GGCC	4 cut(s)	193, 342, 477, 1518
BspCNI	CTCAG	5 cut(s)	390, 828, 1257, 1672, 2242
BspEI	TCCGGA	1 cut(s)	225
BspLI	GGNNCC	5 cut(s)	168, 230, 659, 881, 1843
BspPI	GGATC	7 cut(s)	262, 652, 665, 1119, 1566, 2154, 2333
BspQI	GCTCTTC	3 cut(s)	909, 1746, 2234
BspTI	CTTAAG	1 cut(s)	2336
BsrBI	CCGCTC	1 cut(s)	1852
BsrDI	GCAATG	1 cut(s)	221
BsrFI	RCCGGY	1 cut(s)	445
BsrI	ACTGG	6 cut(s)	667, 1032, 1404, 1613, 1663, 2275
BssAI	RCCGGY	1 cut(s)	445
BssECI	CCNNGG	7 cut(s)	162, 202, 863, 1094, 1160, 1227, 2290
BssSI	CACGAG	1 cut(s)	1699
BssT1I	CCWWGG	6 cut(s)	162, 863, 1094, 1160, 1227, 2290
Bst2BI	CACGAG	1 cut(s)	1699
Bst2UI	CCWGG	2 cut(s)	240, 331
Bst4CI	ACNGT	6 cut(s)	778, 856, 1346, 1369, 1432, 1971
Bst6I	CTCTTC	5 cut(s)	384, 909, 1734, 1746, 2234
BstAFI	CTTAAG	1 cut(s)	2336
BstAPI	GCANNNNNTGC	2 cut(s)	220, 790
BstC8I	GCNNGC	6 cut(s)	159, 212, 310, 479, 1101, 2106
BstDEI	CTNAG	7 cut(s)	398, 836, 1085, 1244, 1680, 1748, 2250
BstDSI	CCRYGG	4 cut(s)	162, 1094, 1160, 1227
BstF5I	GGATG	8 cut(s)	115, 212, 663, 1090, 1156, 1396, 1607, 1960
BstMAI	GTCTC	6 cut(s)	135, 529, 575, 832, 1390, 2033
BstMWI	GCNNNNNNNGC	3 cut(s)	158, 220, 790
BstNI	CCWGG	2 cut(s)	240, 331
BstNSI	RCATGY	2 cut(s)	485, 2022
BstSCI	CCNGG	3 cut(s)	201, 238, 329
BstSFI	CTRYAG	1 cut(s)	1707
BstSLI	GKGCMC	2 cut(s)	909, 1781
BstV1I	GCAGC	6 cut(s)	401, 1200, 1519, 1699, 1748, 2088
BstV2I	GAAGAC	1 cut(s)	707
BstX2I	RGATCY	4 cut(s)	657, 967, 1558, 2325
BstXI	CCANNNNNNTGG	1 cut(s)	752
BstYI	RGATCY	4 cut(s)	657, 967, 1558, 2325
BsuI	GTATCC	1 cut(s)	1520
BsuRI	GGCC	4 cut(s)	193, 342, 477, 1518
BtgI	CCRYGG	4 cut(s)	162, 1094, 1160, 1227
BtsCI	GGATG	8 cut(s)	115, 212, 663, 1090, 1156, 1396, 1607, 1960
BtsI	GCAGTG	2 cut(s)	147, 345
BtsIMutI	CAGTG	4 cut(s)	147, 345, 584, 2045
Cac8I	GCNNGC	6 cut(s)	159, 212, 310, 479, 1101, 2106
CaiI	CAGNNNCTG	1 cut(s)	1975
Cfr10I	RCCGGY	1 cut(s)	445
Cfr13I	GGNCC	1 cut(s)	192
Csp6I	GTAC	6 cut(s)	279, 434, 1684, 2184, 2265, 2388
CviQI	GTAC	6 cut(s)	279, 434, 1684, 2184, 2265, 2388
DdeI	CTNAG	7 cut(s)	398, 836, 1085, 1244, 1680, 1748, 2250
DraI	TTTAAA	1 cut(s)	1546
EaeI	YGGCCR	1 cut(s)	340
Eam1104I	CTCTTC	5 cut(s)	384, 909, 1734, 1746, 2234
EarI	CTCTTC	5 cut(s)	384, 909, 1734, 1746, 2234
Ecl136II	GAGCTC	1 cut(s)	1704
Eco130I	CCWWGG	6 cut(s)	162, 863, 1094, 1160, 1227, 2290
Eco147I	AGGCCT	2 cut(s)	477, 1518
Eco24I	GRGCYC	1 cut(s)	1706
Eco53kI	GAGCTC	1 cut(s)	1704
Eco88I	CYCGRG	1 cut(s)	422
EcoICRI	GAGCTC	1 cut(s)	1704
EcoRI	GAATTC	2 cut(s)	733, 1766
EcoRII	CCWGG	2 cut(s)	238, 329
EcoT14I	CCWWGG	6 cut(s)	162, 863, 1094, 1160, 1227, 2290
EcoT22I	ATGCAT	2 cut(s)	1136, 2083
EcoT38I	GRGCYC	1 cut(s)	1706
ErhI	CCWWGG	6 cut(s)	162, 863, 1094, 1160, 1227, 2290
Esp3I	CGTCTC	1 cut(s)	2033
FaqI	GGGAC	3 cut(s)	579, 778, 1317
FauI	CCCGC	1 cut(s)	1845
FbaI	TGATCA	1 cut(s)	97
FblI	GTMKAC	2 cut(s)	266, 1065
Fnu4HI	GCNGC	6 cut(s)	415, 1214, 1508, 1713, 1737, 2102
FokI	GGATG	8 cut(s)	122, 219, 650, 1077, 1143, 1403, 1614, 1967
FriOI	GRGCYC	1 cut(s)	1706
Fsp4HI	GCNGC	6 cut(s)	415, 1214, 1508, 1713, 1737, 2102
FspBI	CTAG	6 cut(s)	800, 821, 1005, 1061, 2163, 2262
GluI	GCNGC	6 cut(s)	415, 1214, 1508, 1713, 1737, 2102
GsuI	CTGGAG	1 cut(s)	352
HaeIII	GGCC	4 cut(s)	193, 342, 477, 1518
HapII	CCGG	5 cut(s)	202, 226, 446, 611, 981
HincII	GTYRAC	2 cut(s)	267, 457
HindII	GTYRAC	2 cut(s)	267, 457
HindIII	AAGCTT	2 cut(s)	603, 2382
HinfI	GANTC	7 cut(s)	31, 263, 301, 715, 815, 1925, 2087
HpaII	CCGG	5 cut(s)	202, 226, 446, 611, 981
HphI	GGTGA	4 cut(s)	316, 736, 790, 2002
Hpy166II	GTNNAC	6 cut(s)	267, 457, 907, 1066, 1754, 2024
Hpy188I	TCNGA	8 cut(s)	85, 262, 967, 1051, 1247, 1262, 1390, 2058
Hpy188III	TCNNGA	7 cut(s)	176, 226, 422, 564, 599, 1976, 2236
Hpy8I	GTNNAC	6 cut(s)	267, 457, 907, 1066, 1754, 2024
HpyAV	CCTTC	7 cut(s)	37, 144, 1389, 1451, 1697, 1891, 2204
HpyCH4III	ACNGT	6 cut(s)	778, 856, 1346, 1369, 1432, 1971
HpyCH4IV	ACGT	2 cut(s)	929, 2026
HpyF10VI	GCNNNNNNNGC	3 cut(s)	158, 220, 790
HpyF3I	CTNAG	7 cut(s)	398, 836, 1085, 1244, 1680, 1748, 2250
HpySE526I	ACGT	2 cut(s)	929, 2026
Kpn2I	TCCGGA	1 cut(s)	225
Ksp22I	TGATCA	1 cut(s)	97
LguI	GCTCTTC	3 cut(s)	909, 1746, 2234
LmnI	GCTCC	2 cut(s)	228, 1650
Lsp1109I	GCAGC	6 cut(s)	401, 1200, 1519, 1699, 1748, 2088
LweI	GCATC	9 cut(s)	197, 579, 971, 991, 1138, 1195, 1370, 1441, 1621
MaeI	CTAG	6 cut(s)	800, 821, 1005, 1061, 2163, 2262
MaeII	ACGT	2 cut(s)	929, 2026
MaeIII	GTNAC	4 cut(s)	1278, 1310, 1426, 1604
MbiI	CCGCTC	1 cut(s)	1852
MflI	RGATCY	4 cut(s)	657, 967, 1558, 2325
MhlI	GDGCHC	5 cut(s)	225, 909, 921, 1706, 1781
MlsI	TGGCCA	1 cut(s)	342
MluNI	TGGCCA	1 cut(s)	342
MlyI	GAGTC	4 cut(s)	25, 272, 295, 2081
MmeI	TCCRAC	3 cut(s)	1089, 2227, 2384
Mox20I	TGGCCA	1 cut(s)	342
Mph1103I	ATGCAT	2 cut(s)	1136, 2083
MroI	TCCGGA	1 cut(s)	225
MroXI	GAANNNNTTC	1 cut(s)	2224
MscI	TGGCCA	1 cut(s)	342
MseI	TTAA	7 cut(s)	27, 48, 1074, 1545, 1634, 2138, 2337
MslI	CAYNNNNRTG	3 cut(s)	93, 381, 1577
Msp20I	TGGCCA	1 cut(s)	342
MspA1I	CMGCKG	1 cut(s)	1510
MspCI	CTTAAG	1 cut(s)	2336
MspI	CCGG	5 cut(s)	202, 226, 446, 611, 981
MspR9I	CCNGG	3 cut(s)	203, 240, 331
MvaI	CCWGG	2 cut(s)	240, 331
MwoI	GCNNNNNNNGC	3 cut(s)	158, 220, 790
NciI	CCSGG	1 cut(s)	203
NcoI	CCATGG	4 cut(s)	162, 1094, 1160, 1227
NlaIV	GGNNCC	5 cut(s)	168, 230, 659, 881, 1843
NmuCI	GTSAC	2 cut(s)	1310, 1604
NsiI	ATGCAT	2 cut(s)	1136, 2083
NspI	RCATGY	2 cut(s)	485, 2022
PceI	AGGCCT	2 cut(s)	477, 1518
PciSI	GCTCTTC	3 cut(s)	909, 1746, 2234
PdmI	GAANNNNTTC	1 cut(s)	2224
PfeI	GAWTC	3 cut(s)	715, 815, 1925
PkrI	GCNGC	6 cut(s)	416, 1215, 1509, 1714, 1738, 2103
PleI	GAGTC	4 cut(s)	25, 271, 295, 2081
PpsI	GAGTC	4 cut(s)	25, 271, 295, 2081
PsiI	TTATAA	1 cut(s)	621
Psp124BI	GAGCTC	1 cut(s)	1706
Psp1406I	AACGTT	1 cut(s)	929
Psp6I	CCWGG	2 cut(s)	238, 329
PspGI	CCWGG	2 cut(s)	238, 329
PspN4I	GGNNCC	5 cut(s)	168, 230, 659, 881, 1843
PspPI	GGNCC	1 cut(s)	192
PsrI	GAACNNNNNNTAC	4 cut(s)	1648, 1680, 2251, 2283
PstNI	CAGNNNCTG	1 cut(s)	1975
PsuI	RGATCY	4 cut(s)	657, 967, 1558, 2325
RsaI	GTAC	6 cut(s)	280, 435, 1685, 2185, 2266, 2389
RsaNI	GTAC	6 cut(s)	279, 434, 1684, 2184, 2265, 2388
RseI	CAYNNNNRTG	3 cut(s)	93, 381, 1577
SacI	GAGCTC	1 cut(s)	1706
SalI	GTCGAC	1 cut(s)	265
SapI	GCTCTTC	3 cut(s)	909, 1746, 2234
SaqAI	TTAA	7 cut(s)	27, 48, 1074, 1545, 1634, 2138, 2337
SatI	GCNGC	6 cut(s)	415, 1214, 1508, 1713, 1737, 2102
Sau96I	GGNCC	1 cut(s)	192
ScaI	AGTACT	1 cut(s)	2266
SchI	GAGTC	4 cut(s)	25, 272, 295, 2081
ScrFI	CCNGG	3 cut(s)	203, 240, 331
SduI	GDGCHC	5 cut(s)	225, 909, 921, 1706, 1781
SfaNI	GCATC	9 cut(s)	197, 579, 971, 991, 1138, 1195, 1370, 1441, 1621
SfcI	CTRYAG	1 cut(s)	1707
SmiMI	CAYNNNNRTG	3 cut(s)	93, 381, 1577
SmlI	CTYRAG	3 cut(s)	1553, 1772, 2336
SmoI	CTYRAG	3 cut(s)	1553, 1772, 2336
SpeI	ACTAGT	1 cut(s)	2261
SseBI	AGGCCT	2 cut(s)	477, 1518
SsiI	CCGC	5 cut(s)	684, 1338, 1454, 1510, 1852
SspMI	CTAG	6 cut(s)	800, 821, 1005, 1061, 2163, 2262
SstI	GAGCTC	1 cut(s)	1706
StuI	AGGCCT	2 cut(s)	477, 1518
StyD4I	CCNGG	3 cut(s)	201, 238, 329
StyI	CCWWGG	6 cut(s)	162, 863, 1094, 1160, 1227, 2290
TaaI	ACNGT	6 cut(s)	778, 856, 1346, 1369, 1432, 1971
TaiI	ACGT	2 cut(s)	932, 2029
TaqI	TCGA	6 cut(s)	266, 299, 1199, 1566, 1817, 2237
TatI	WGTACW	3 cut(s)	433, 2264, 2387
TfiI	GAWTC	3 cut(s)	715, 815, 1925
Tru1I	TTAA	7 cut(s)	27, 48, 1074, 1545, 1634, 2138, 2337
Tru9I	TTAA	7 cut(s)	27, 48, 1074, 1545, 1634, 2138, 2337
TscAI	CASTG	4 cut(s)	154, 352, 591, 2045
TseFI	GTSAC	2 cut(s)	1310, 1604
TseI	GCWGC	6 cut(s)	414, 1213, 1507, 1712, 1736, 2101
Tsp45I	GTSAC	2 cut(s)	1310, 1604
TspRI	CASTG	4 cut(s)	154, 352, 591, 2045
Vha464I	CTTAAG	1 cut(s)	2336
VneI	GTGCAC	1 cut(s)	905
XapI	RAATTY	4 cut(s)	727, 733, 1045, 1766
XceI	RCATGY	2 cut(s)	485, 2022
XcmI	CCANNNNNNNNNTGG	2 cut(s)	246, 2177
XmiI	GTMKAC	2 cut(s)	266, 1065
XmnI	GAANNNNTTC	1 cut(s)	2224
XspI	CTAG	6 cut(s)	800, 821, 1005, 1061, 2163, 2262
ZrmI	AGTACT	1 cut(s)	2266
Zsp2I	ATGCAT	2 cut(s)	1136, 2083

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.