RchiOBHm_Chr1g0320561

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0010536
Physical Location & Seq
Forward (+)
8226813 .. 8227919
1107 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 381 bp
ATGGAGGTACAGGTGGTGTCATTAGGAACAATCAAGGATGACTTTGTCAGCTCTCCCTTACACGCTGAACTTCGGGCTTTGAAATATGGCTTGGAGTTTCTGCAGGCTATGAATGTTATCAAGGTGGTTGTAGAGAGTGATTATCTAGTAGCAGTCCAGGCCATTAACTCTTTGGGTGGAGATTTGTCCGACTTAGGTGCTCTGATATCTGATATTCAAGGCCTTGTGGCTGCTGTGGGGGAGGTTACAGTTAACTTTGCACCCAGGCAAGCAAACATGGTTGCTCATCATTTAGCTAGCTATAGTTTTGACTCAAACCTCCGTCTTGAATGGTTTGTTAATGCTCCAGAGTTTTTTGGATGCCCTCATGTACAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

126

Amino Acids

13.69

Weight (kDa)

4.65

Isoelectric Point (pI)

21.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 16 - 101 1.8e-14 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 379
AfaI GTAC 2 cut(s) 9, 372
AgsI TTSAA 3 cut(s) 82, 218, 329
AjnI CCWGG 2 cut(s) 156, 263
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AluBI AGCT 3 cut(s) 51, 296, 300
AluI AGCT 3 cut(s) 51, 296, 300
Alw21I GWGCWC 1 cut(s) 202
AoxI GGCC 2 cut(s) 159, 220
ApeKI GCWGC 1 cut(s) 230
AsuNHI GCTAGC 1 cut(s) 296
Bbv12I GWGCWC 1 cut(s) 202
BbvI GCAGC 1 cut(s) 217
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BciT130I CCWGG 2 cut(s) 158, 265
BfaI CTAG 2 cut(s) 146, 297
BfmI CTRYAG 2 cut(s) 101, 301
BisI GCNGC 1 cut(s) 231
BlsI GCNGC 1 cut(s) 232
Bme1390I CCNGG 2 cut(s) 158, 265
BmrFI CCNGG 2 cut(s) 158, 265
BmsI GCATC 1 cut(s) 350
BmtI GCTAGC 1 cut(s) 300
BpmI CTGGAG 1 cut(s) 330
BsaJI CCNNGG 1 cut(s) 263
BseBI CCWGG 2 cut(s) 158, 265
BseDI CCNNGG 1 cut(s) 263
BseGI GGATG 2 cut(s) 43, 365
BseXI GCAGC 1 cut(s) 217
BshFI GGCC 2 cut(s) 161, 222
BsiHKAI GWGCWC 1 cut(s) 202
BsnI GGCC 2 cut(s) 161, 222
Bsp1286I GDGCHC 1 cut(s) 202
Bsp1407I TGTACA 1 cut(s) 370
BspANI GGCC 2 cut(s) 161, 222
BspMAI CTGCAG 1 cut(s) 105
BspOI GCTAGC 1 cut(s) 300
BsrGI TGTACA 1 cut(s) 370
BssECI CCNNGG 1 cut(s) 263
Bst2UI CCWGG 2 cut(s) 158, 265
Bst4CI ACNGT 1 cut(s) 250
BstAUI TGTACA 1 cut(s) 370
BstC8I GCNNGC 3 cut(s) 105, 270, 298
BstDEI CTNAG 1 cut(s) 193
BstF5I GGATG 2 cut(s) 43, 365
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstNI CCWGG 2 cut(s) 158, 265
BstSCI CCNGG 2 cut(s) 156, 263
BstSFI CTRYAG 2 cut(s) 101, 301
BstV1I GCAGC 1 cut(s) 217
BsuRI GGCC 2 cut(s) 161, 222
BtsCI GGATG 2 cut(s) 43, 365
Cac8I GCNNGC 3 cut(s) 105, 270, 298
Csp6I GTAC 2 cut(s) 8, 371
CviAII CATG 2 cut(s) 277, 368
CviJI RGCY 9 cut(s) 51, 77, 90, 107, 161, 222, 230, 296, 300
CviKI_1 RGCY 9 cut(s) 51, 77, 90, 107, 161, 222, 230, 296, 300
CviQI GTAC 2 cut(s) 8, 371
DdeI CTNAG 1 cut(s) 193
Eco147I AGGCCT 1 cut(s) 222
Eco32I GATATC 1 cut(s) 207
EcoRII CCWGG 2 cut(s) 156, 263
EcoRV GATATC 1 cut(s) 207
FaeI CATG 2 cut(s) 280, 371
FaiI YATR 6 cut(s) 87, 110, 278, 303, 369, 379
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FatI CATG 2 cut(s) 276, 367
Fnu4HI GCNGC 1 cut(s) 231
FokI GGATG 2 cut(s) 50, 372
Fsp4HI GCNGC 1 cut(s) 231
FspBI CTAG 2 cut(s) 146, 297
GluI GCNGC 1 cut(s) 231
GsuI CTGGAG 1 cut(s) 330
HaeIII GGCC 2 cut(s) 161, 222
Hin1II CATG 2 cut(s) 280, 371
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 1 cut(s) 311
HpaI GTTAAC 1 cut(s) 253
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 3 cut(s) 190, 204, 211
Hpy188III TCNNGA 2 cut(s) 326, 347
Hpy8I GTNNAC 1 cut(s) 253
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4V TGCA 2 cut(s) 103, 260
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 1 cut(s) 193
Hsp92II CATG 2 cut(s) 280, 371
KspAI GTTAAC 1 cut(s) 253
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 6 cut(s) 89, 143, 170, 250, 277, 360
Lsp1109I GCAGC 1 cut(s) 217
LweI GCATC 1 cut(s) 350
MaeI CTAG 2 cut(s) 146, 297
MaeIII GTNAC 1 cut(s) 244
MhlI GDGCHC 1 cut(s) 202
MluCI AATT 1 cut(s) 374
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 3 cut(s) 235, 329, 375
MseI TTAA 3 cut(s) 165, 252, 339
MspR9I CCNGG 2 cut(s) 158, 265
MvaI CCWGG 2 cut(s) 158, 265
MwoI GCNNNNNNNGC 1 cut(s) 158
NheI GCTAGC 1 cut(s) 296
NlaIII CATG 2 cut(s) 280, 371
PceI AGGCCT 1 cut(s) 222
PflFI GACNNNGTC 1 cut(s) 44
PkrI GCNGC 1 cut(s) 232
PleI GAGTC 1 cut(s) 305
PpsI GAGTC 1 cut(s) 305
PsiI TTATAA 1 cut(s) 379
Psp6I CCWGG 2 cut(s) 156, 263
PspGI CCWGG 2 cut(s) 156, 263
PstI CTGCAG 1 cut(s) 105
PsyI GACNNNGTC 1 cut(s) 44
RsaI GTAC 2 cut(s) 9, 372
RsaNI GTAC 2 cut(s) 8, 371
SaqAI TTAA 3 cut(s) 165, 252, 339
SatI GCNGC 1 cut(s) 231
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 2 cut(s) 158, 265
SduI GDGCHC 1 cut(s) 202
SetI ASST 9 cut(s) 9, 15, 53, 126, 199, 246, 298, 302, 321
SfaNI GCATC 1 cut(s) 350
SfcI CTRYAG 2 cut(s) 101, 301
Sse9I AATT 1 cut(s) 374
SseBI AGGCCT 1 cut(s) 222
SspMI CTAG 2 cut(s) 146, 297
StuI AGGCCT 1 cut(s) 222
StyD4I CCNGG 2 cut(s) 156, 263
TaaI ACNGT 1 cut(s) 250
TasI AATT 1 cut(s) 374
TatI WGTACW 1 cut(s) 370
Tru1I TTAA 3 cut(s) 165, 252, 339
Tru9I TTAA 3 cut(s) 165, 252, 339
TseI GCWGC 1 cut(s) 230
TspDTI ATGAA 1 cut(s) 125
TspGWI ACGGA 1 cut(s) 311
Tth111I GACNNNGTC 1 cut(s) 44
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XspI CTAG 2 cut(s) 146, 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.