RchiOBHm_Chr1g0325791

Nudix hydrolase 16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0006776
Physical Location & Seq
Forward (+)
13842080 .. 13848107
6028 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 525 bp
ATGTGTGATATAGTGGCCCGTACGGGTCGTCATCAGCAACGCTACGAGGCCGGGTGTCGCCTTGTTGCCGGGTGTATTCCATTCAGGTACAGAAATTCTGATGAGAGTGGCGATGCTGATTCTGAGAAGATTGTTGAGGTCCTTATGATTAACTCATCCAGTGGACCAGGTCTTTTATTTCCTAAGGGAGGCTGGGAGAATGATGAAACTGTGCAGGAAGCTGCCGTAAGAGAAGCTATTGAAGAAGCTGGGGTTCGAGGAGAACTAATGGATTTAGTGGGGTATTACCAATTTAAGAGTAAAACTCACCAGGATGAGTTAAACCCAGAAGGTATATGTAGAGCTGCTATGTTTGCATTGTTCGTCAAGGAGGAGCTTGAGTCATGGCCAGAACAGAGCACTCGAAACAGAACTTGGCTGACTGTTCAAGAGGCTACTGGTAGATGCCGGCATGCATGGATGAGTGAGGCCCTGGTGGAGGGTTTCTCAAAGTGGCATGCAGAAAAGATGATAGGTGCAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

174

Amino Acids

19.58

Weight (kDa)

5.1

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 47 - 145 2.6e-13 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 386
AcsI RAATTY 1 cut(s) 94
AfaI GTAC 2 cut(s) 22, 89
AfiI CCNNNNNNNGG 2 cut(s) 188, 478
AgsI TTSAA 2 cut(s) 242, 428
AjnI CCWGG 3 cut(s) 166, 309, 471
AjuI GAANNNNNNNTTGG 2 cut(s) 397, 429
AluBI AGCT 5 cut(s) 221, 236, 248, 344, 376
AluI AGCT 5 cut(s) 221, 236, 248, 344, 376
Alw21I GWGCWC 1 cut(s) 401
AoxI GGCC 4 cut(s) 15, 48, 386, 468
ApeKI GCWGC 2 cut(s) 221, 344
ApoI RAATTY 1 cut(s) 94
AspS9I GGNCC 4 cut(s) 16, 139, 164, 469
AsuC2I CCSGG 2 cut(s) 52, 70
AsuHPI GGTGA 1 cut(s) 299
AvaII GGWCC 2 cut(s) 139, 164
AxyI CCTNAGG 1 cut(s) 183
BalI TGGCCA 1 cut(s) 388
Bbv12I GWGCWC 1 cut(s) 401
BbvI GCAGC 2 cut(s) 208, 331
BceAI ACGGC 1 cut(s) 209
BciT130I CCWGG 3 cut(s) 168, 311, 473
BcnI CCSGG 2 cut(s) 52, 70
BisI GCNGC 2 cut(s) 222, 345
BlsI GCNGC 2 cut(s) 223, 346
Bme1390I CCNGG 5 cut(s) 52, 70, 168, 311, 473
Bme18I GGWCC 2 cut(s) 139, 164
BmgT120I GGNCC 4 cut(s) 16, 139, 164, 469
BmrFI CCNGG 5 cut(s) 52, 70, 168, 311, 473
BmsI GCATC 2 cut(s) 103, 434
BplI GAGNNNNNCTC 4 cut(s) 289, 321, 470, 502
BpuEI CTTGAG 1 cut(s) 398
BpuMI CCSGG 2 cut(s) 52, 70
BsaJI CCNNGG 1 cut(s) 471
BsaXI ACNNNNNCTCC 2 cut(s) 365, 395
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 478
Bse118I RCCGGY 1 cut(s) 447
Bse1I ACTGG 2 cut(s) 159, 442
Bse21I CCTNAGG 1 cut(s) 183
BseBI CCWGG 3 cut(s) 168, 311, 473
BseDI CCNNGG 1 cut(s) 471
BseGI GGATG 3 cut(s) 155, 319, 465
BseLI CCNNNNNNNGG 2 cut(s) 188, 478
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 2 cut(s) 159, 442
BseRI GAGGAG 2 cut(s) 273, 386
BseXI GCAGC 2 cut(s) 208, 331
BseYI CCCAGC 2 cut(s) 192, 248
BsgI GTGCAG 1 cut(s) 233
BshFI GGCC 4 cut(s) 17, 50, 388, 470
BsiHKAI GWGCWC 1 cut(s) 401
BsiSI CCGG 3 cut(s) 51, 69, 448
BsiWI CGTACG 1 cut(s) 20
BslI CCNNNNNNNGG 2 cut(s) 188, 478
BsnI GGCC 4 cut(s) 17, 50, 388, 470
Bsp1286I GDGCHC 1 cut(s) 401
BspANI GGCC 4 cut(s) 17, 50, 388, 470
BspCNI CTCAG 1 cut(s) 115
BsrFI RCCGGY 1 cut(s) 447
BsrI ACTGG 2 cut(s) 159, 442
BssAI RCCGGY 1 cut(s) 447
BssECI CCNNGG 1 cut(s) 471
Bst2UI CCWGG 3 cut(s) 168, 311, 473
Bst4CI ACNGT 2 cut(s) 211, 424
BstC8I GCNNGC 3 cut(s) 449, 453, 498
BstDEI CTNAG 2 cut(s) 123, 183
BstENI CCTNNNNNAGG 2 cut(s) 186, 476
BstF5I GGATG 3 cut(s) 155, 319, 465
BstMWI GCNNNNNNNGC 1 cut(s) 353
BstNI CCWGG 3 cut(s) 168, 311, 473
BstNSI RCATGY 2 cut(s) 455, 500
BstSCI CCNGG 5 cut(s) 50, 68, 166, 309, 471
BstV1I GCAGC 2 cut(s) 208, 331
Bsu36I CCTNAGG 1 cut(s) 183
BsuRI GGCC 4 cut(s) 17, 50, 388, 470
BtgZI GCGATG 1 cut(s) 126
BtsCI GGATG 3 cut(s) 155, 319, 465
BtsIMutI CAGTG 1 cut(s) 166
Cac8I GCNNGC 3 cut(s) 449, 453, 498
Cfr10I RCCGGY 1 cut(s) 447
Cfr13I GGNCC 4 cut(s) 16, 139, 164, 469
CsiI ACCWGGT 1 cut(s) 166
Csp6I GTAC 2 cut(s) 21, 88
CviAII CATG 4 cut(s) 384, 452, 456, 497
CviQI GTAC 2 cut(s) 21, 88
DdeI CTNAG 2 cut(s) 123, 183
EaeI YGGCCR 1 cut(s) 386
Eco47I GGWCC 2 cut(s) 139, 164
Eco81I CCTNAGG 1 cut(s) 183
EcoNI CCTNNNNNAGG 2 cut(s) 186, 476
EcoO109I RGGNCCY 2 cut(s) 139, 469
EcoRII CCWGG 3 cut(s) 166, 309, 471
EcoT22I ATGCAT 1 cut(s) 457
FaeI CATG 4 cut(s) 387, 455, 459, 500
FaiI YATR 9 cut(s) 11, 146, 335, 337, 350, 385, 453, 457, 498
FatI CATG 4 cut(s) 383, 451, 455, 496
Fnu4HI GCNGC 2 cut(s) 222, 345
FokI GGATG 3 cut(s) 142, 326, 472
Fsp4HI GCNGC 2 cut(s) 222, 345
GluI GCNGC 2 cut(s) 222, 345
GsaI CCCAGC 2 cut(s) 196, 252
HaeIII GGCC 4 cut(s) 17, 50, 388, 470
HapII CCGG 3 cut(s) 51, 69, 448
Hin1II CATG 4 cut(s) 387, 455, 459, 500
HinfI GANTC 2 cut(s) 119, 380
HpaII CCGG 3 cut(s) 51, 69, 448
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 1 cut(s) 164
Hpy188I TCNGA 2 cut(s) 100, 124
Hpy188III TCNNGA 1 cut(s) 428
Hpy8I GTNNAC 1 cut(s) 164
HpyAV CCTTC 1 cut(s) 323
HpyCH4III ACNGT 2 cut(s) 211, 424
HpyCH4V TGCA 5 cut(s) 214, 356, 455, 500, 518
HpyF10VI GCNNNNNNNGC 1 cut(s) 353
HpyF3I CTNAG 2 cut(s) 123, 183
Hsp92II CATG 4 cut(s) 387, 455, 459, 500
KroI GCCGGC 1 cut(s) 447
KroNI GCCGGC 1 cut(s) 449
LmnI GCTCC 1 cut(s) 373
Lsp1109I GCAGC 2 cut(s) 208, 331
LweI GCATC 2 cut(s) 103, 434
MabI ACCWGGT 1 cut(s) 166
MboII GAAGA 2 cut(s) 139, 254
MhlI GDGCHC 1 cut(s) 401
MlsI TGGCCA 1 cut(s) 388
MluCI AATT 2 cut(s) 94, 290
MluNI TGGCCA 1 cut(s) 388
MlyI GAGTC 1 cut(s) 389
MnlI CCTC 8 cut(s) 40, 130, 182, 251, 364, 424, 460, 472
Mox20I TGGCCA 1 cut(s) 388
Mph1103I ATGCAT 1 cut(s) 457
MroNI GCCGGC 1 cut(s) 447
MscI TGGCCA 1 cut(s) 388
MseI TTAA 3 cut(s) 150, 294, 320
MslI CAYNNNNRTG 1 cut(s) 312
Msp20I TGGCCA 1 cut(s) 388
MspI CCGG 3 cut(s) 51, 69, 448
MspR9I CCNGG 5 cut(s) 52, 70, 168, 311, 473
MvaI CCWGG 3 cut(s) 168, 311, 473
MwoI GCNNNNNNNGC 1 cut(s) 353
NaeI GCCGGC 1 cut(s) 449
NciI CCSGG 2 cut(s) 52, 70
NgoMIV GCCGGC 1 cut(s) 447
NlaIII CATG 4 cut(s) 387, 455, 459, 500
NsiI ATGCAT 1 cut(s) 457
NspI RCATGY 2 cut(s) 455, 500
PaeI GCATGC 2 cut(s) 455, 500
PdiI GCCGGC 1 cut(s) 449
PfeI GAWTC 1 cut(s) 119
Pfl23II CGTACG 1 cut(s) 20
PflFI GACNNNGTC 1 cut(s) 168
PkrI GCNGC 2 cut(s) 223, 346
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
PpuMI RGGWCCY 1 cut(s) 139
Psp5II RGGWCCY 1 cut(s) 139
Psp6I CCWGG 3 cut(s) 166, 309, 471
PspFI CCCAGC 2 cut(s) 192, 248
PspGI CCWGG 3 cut(s) 166, 309, 471
PspLI CGTACG 1 cut(s) 20
PspPI GGNCC 4 cut(s) 16, 139, 164, 469
PspPPI RGGWCCY 1 cut(s) 139
PsyI GACNNNGTC 1 cut(s) 168
RsaI GTAC 2 cut(s) 22, 89
RsaNI GTAC 2 cut(s) 21, 88
RseI CAYNNNNRTG 1 cut(s) 312
SaqAI TTAA 3 cut(s) 150, 294, 320
SatI GCNGC 2 cut(s) 222, 345
Sau96I GGNCC 4 cut(s) 16, 139, 164, 469
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 5 cut(s) 52, 70, 168, 311, 473
SduI GDGCHC 1 cut(s) 401
SexAI ACCWGGT 1 cut(s) 166
SfaNI GCATC 2 cut(s) 103, 434
SinI GGWCC 2 cut(s) 139, 164
SmiMI CAYNNNNRTG 1 cut(s) 312
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SphI GCATGC 2 cut(s) 455, 500
Sse9I AATT 2 cut(s) 94, 290
StyD4I CCNGG 5 cut(s) 50, 68, 166, 309, 471
TaaI ACNGT 2 cut(s) 211, 424
TaqI TCGA 2 cut(s) 256, 403
TasI AATT 2 cut(s) 94, 290
TfiI GAWTC 1 cut(s) 119
Tru1I TTAA 3 cut(s) 150, 294, 320
Tru9I TTAA 3 cut(s) 150, 294, 320
TscAI CASTG 1 cut(s) 166
TseI GCWGC 2 cut(s) 221, 344
TspDTI ATGAA 1 cut(s) 219
TspRI CASTG 1 cut(s) 166
Tth111I GACNNNGTC 1 cut(s) 168
VpaK11BI GGWCC 2 cut(s) 139, 164
XagI CCTNNNNNAGG 2 cut(s) 186, 476
XapI RAATTY 1 cut(s) 94
XceI RCATGY 2 cut(s) 455, 500
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.