RchiOBHm_Chr1g0332961

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0009713
Physical Location & Seq
Reverse (-)
24845529 .. 24851656
6128 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3030 bp
ATGCAACCTAGGAGGCACGAGAGTTTCAATAGACAGCAGCCTCCGGCTTCTAAATTACGTCCCACCTACCGATTCGAATCCGGTGCCAACAACCACCCTTATAGTAGTGGTAGTAGGAGGAGACCTGAGGAGGTACATCGGAATATTAGGAGGAGTTTGAGCCCGCAACAAGATCATCTGGGTGTCAGTCAAAGGAATGTTGCTTCTATTCAGAGGGAGTATGGCTGGCATTTGGGCCGCGGTGGTGGCGGTAGGACTGATGGCGTCCGCCGCCGCAGGTCAACATCCGGGTCACCCCTACTGCCTTTTGGTGAAATGCGAAAAATGTCACAATTTGAGGAGGAGGAGGAGGATGGGGATTTGCGGAGGAGTTATTCACCACCACCACAACCACCGGTACCTGTTCCGGCATCTGTTCCTTTGGAGTTAAAACACAGACGTGATAGCGTTGAGGCTGGAAATTATAGTGTCAATGATGATTTGAATGGTAGGAGAGTTTATGGCTCCGAGCATAATGATTTTGGAATCAGTAAAGAAGATTTGAATGAAAGCAGATTGACAGCTGGTGGTAAACATGGGACATTGGGTCAAAAACCAATCCATATGGAAGATAGTGCTGTAAGAGGATCCAAAACCATGTTTATCAAAGGTGACACCGTGCATGGAGCATATCATTCTTCTCCAAGCCTTGGTACTGTTATAACTCAAGGAGCAAGGGGTGGCCACCTACCATCATCAACGCGCAGCATGAGCATACGTCATTCTGACCAAGAAAGGCTTCAGTATGCGGACCCTATATCTTTGGATAGATTACCAGTGACAGAAGCCTACAAAGAAGGAAATAAACCCGTTTTTCCTACAAGAGATGGCTTTTATTCCATGATGTCTGGATCTCATTCCAAGAATTATTTTGCTAGCTCCTCAACAGGGTTGAGGAATGAATTCCAAGATTCCTATCAGCATGAGCAGCATATGCCTTCTTTGGAAGAATTTTCAAGAAGTAGTAGAAAACTTAAAGATTCTGTGAGCATTAATGCATATAGGGAAAGGCCGGTAGTAGAGTTTTCTCGAGACCCTGATTCTGGGCAGAAGAATCTGACATTTTACCAGAGAGATAGTCCTACTAGAGTTGAGTATGATGATGATTATTATTTCTACCCAAACTCAAGAGGAATGATATTTGACGATCGTGAGCTTCCATCTGATCACTTACACAAAGTGATGCATCCACGAGCACCACTTGACTATGACCATTCTCGGATGGGTTATGATCATAGAACTGTGTCAAGATCTAGTACCATGCTCCCTGTTGTGGAAAGAATTGATAATACTGAGGATTATTCTGGAAATTCAAGGAAGCGCATTATGCTGAATAATTCTACTTTAGAAAGGCATACACTATCAGACTACCCTGATATAAGCAGAATATCAAATGCATCAAAGCAGAGTGGAGAATATATGGGTTCTGGACGCATGCATGACAAGTTTGGAAGGAGAATTTCACAAGATTATGAAACATCGCATTTGGGTGCAGCACAAGTTTGTCAAATCTCACATCTGAAAGAAGATTATGGTTATGAAAGAGATGGAAACCTGAAGTTTCAGGACAGACAGTGTCCTGTGTCTAAATATGACCCAGAAATGCATAGAAATACTATTAAAATGCAGAGTATGAGAGACAAGCTTGGGGTTTATGAACCATCAGATAGGGTGGTGAAAAGAAAATATGTTAATGAAGAGCATGGAAGTATCCAAAATCCTAGAGAAATTATGTCAGGTAAATGGAATACCTCTAGAGAGTTTCAAGATTTGTATGATAGTGGTGAAGAGTGGAATGGTGAAGATGTAGGATCTATGTATACATCTAGATCAGCAAGATTGGGCCATAATGAACACTATAAGGCCCAGAGGAAATATGCTGGAATAGACCAGTATGATGAATTTGCATCTGATGATCGGCTGCCGTCTCAAGACTCTTTGGCAGATGCACAAAGGCACTCAGTTAGATATTATAAACATGGAGATCGGTATGCCAAGGGTCACCAAAACTATGGTTCATTAAGTAGGCATAGGTCACAGCAAGTTGACATAAAAAGTGGCTTTCACAAACAGCACAAAGTTTGGAAGAGAAATGATAATTATCTAGAGGATGTCCACAGAGGTGATGATAATGATGCTGATACATCAGAAAATGGGTTGAGTTCTGCAGCTCCTGAGCCATCAGAGGATTCTGAGGAGTTTATGCAATTGGTACAAGAAGCCTTCCTAACATACTCTAAACAATTAAATATGAACCCTGGTGTTCGGAGAAGATACATGGAACAAGGAAAGGCTGGGACTTTATTCTGCATCGTATGTGGCCGAAGCTTCTCGAAGGAGTTCATGGACACCCAACGATTGGTAACACATGCATTTATGTCTAAAAAAGCTGGATTGAGGACACAACACTTGGGACTTCTCAAAGCAGTTTGTGCTCTGTTGGGGTGGAGTACTGTTATCCCCACTGACATAGTACTATGGGTTCCCCAGGTCTTGCCCAAGGCAGAAGCTTTGGCTCAGAAGGAAGATTTGATTCTTTGGCCTCCAGTCATTATTATCCACAATATTTCTATGTCAGACAACAATCCTGAAAATTGGAAGGTTGTAAGCATTGAAGCTCTTGAGGCTTTTCTAAGAGGTAAAGGTTTAATAAGGGGAAGAATCAAGATGTGCCTGGGGAAGCCTGCTGATCAAAGTGTTGTGTTGGTGAAGTTCTTGGGAACTTTCACTGGGCTTGGGAATGCAGAGAGGATTCATAAGTATTTTGCAGAGCAGAACCGTGGCAGAGCAGACTTTGAGCGAGCAACGTCCAATAACGGAAAAATTGTGGAAGCAGGAATGCAGGTTGACAGTGTGGAGGAGCGATTTTTACATGGATATATGGGGATTGCGGAGGACTTAGATAAAGTAGATTTTAATACCAGGAACTGGAGTTTGATAAAGAGCAAGAAGGAGATTCAGGACCTGGCCAATGCTCCTGTTAAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

1009

Amino Acids

115.28

Weight (kDa)

8.57

Isoelectric Point (pI)

61.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 855 - 981 2.3e-18 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 701, 2013
Acc36I ACCTGC 2 cut(s) 267, 2872
Acc65I GGTACC 1 cut(s) 397
AccB1I GGYRCC 2 cut(s) 83, 397
AccB7I CCANNNNNTGG 3 cut(s) 689, 2398, 2967
AccI GTMKAC 1 cut(s) 1859
AccII CGCG 2 cut(s) 240, 742
AclWI GGATC 4 cut(s) 621, 634, 898, 1858
AcoI YGGCCR 3 cut(s) 721, 2359, 3006
AcsI RAATTY 5 cut(s) 941, 989, 1348, 1497, 1940
AcuI CTGAAG 2 cut(s) 764, 1616
AcyI GRCGYC 1 cut(s) 264
AdeI CACNNNGTG 1 cut(s) 1219
AfaI GTAC 7 cut(s) 135, 399, 694, 1297, 2253, 2491, 2514
AfiI CCNNNNNNNGG 6 cut(s) 689, 927, 1082, 1312, 2398, 2967
AgeI ACCGGT 1 cut(s) 394
AgsI TTSAA 7 cut(s) 28, 484, 544, 996, 1353, 1805, 2654
AhdI GACNNNNNGTC 1 cut(s) 585
AjiI CACGTC 1 cut(s) 440
AjnI CCWGG 5 cut(s) 2296, 2526, 2712, 2960, 3003
AjuI GAANNNNNNNTTGG 2 cut(s) 762, 794
AleI CACNNNNGTG 2 cut(s) 438, 2160
AluBI AGCT 9 cut(s) 563, 918, 1195, 1684, 2210, 2367, 2429, 2549, 2657
AluI AGCT 9 cut(s) 563, 918, 1195, 1684, 2210, 2367, 2429, 2549, 2657
Alw21I GWGCWC 2 cut(s) 1237, 2476
Alw26I GTCTC 4 cut(s) 115, 1065, 1671, 1971
AlwI GGATC 4 cut(s) 621, 634, 898, 1858
AlwNI CAGNNNCTG 3 cut(s) 2213, 2967, 3004
Ama87I CYCGRG 1 cut(s) 1068
AoxI GGCC 8 cut(s) 235, 721, 1049, 1882, 1902, 2359, 2579, 3006
ApeKI GCWGC 6 cut(s) 37, 744, 967, 1532, 1960, 2207
ApoI RAATTY 5 cut(s) 941, 989, 1348, 1497, 1940
AseI ATTAAT 1 cut(s) 1032
AsiGI ACCGGT 1 cut(s) 394
Asp700I GAANNNNTTC 3 cut(s) 777, 941, 2378
Asp718I GGTACC 1 cut(s) 397
AspA2I CCTAGG 1 cut(s) 8
AspLEI GCGC 2 cut(s) 744, 1362
AspS9I GGNCC 5 cut(s) 235, 790, 1882, 1903, 3001
AsuC2I CCSGG 1 cut(s) 289
AsuII TTCGAA 1 cut(s) 75
AsuNHI GCTAGC 1 cut(s) 914
AvaI CYCGRG 1 cut(s) 1068
AvaII GGWCC 2 cut(s) 790, 3001
AvrII CCTAGG 1 cut(s) 8
AxyI CCTNAGG 1 cut(s) 126
BalI TGGCCA 2 cut(s) 723, 3008
BamHI GGATCC 1 cut(s) 626
BanI GGYRCC 2 cut(s) 83, 397
BanII GRGCYC 1 cut(s) 164
BauI CACGAG 2 cut(s) 17, 1230
Bbv12I GWGCWC 2 cut(s) 1237, 2476
BbvI GCAGC 6 cut(s) 49, 756, 979, 1544, 1947, 2219
BccI CCATC 9 cut(s) 254, 347, 739, 860, 1207, 1255, 1580, 1708, 2227
BceAI ACGGC 1 cut(s) 1948
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 5 cut(s) 2298, 2528, 2714, 2962, 3005
BciVI GTATCC 1 cut(s) 1760
BclI TGATCA 3 cut(s) 1204, 1270, 2728
BcnI CCSGG 1 cut(s) 289
BcoDI GTCTC 4 cut(s) 115, 1065, 1671, 1971
BfaI CTAG 8 cut(s) 9, 915, 1125, 1293, 1761, 1794, 1866, 2144
BfmI CTRYAG 1 cut(s) 2205
BfuAI ACCTGC 2 cut(s) 267, 2872
BfuI GTATCC 1 cut(s) 1760
BglII AGATCT 1 cut(s) 1289
BisI GCNGC 9 cut(s) 38, 238, 271, 274, 745, 968, 1533, 1961, 2208
BlnI CCTAGG 1 cut(s) 8
BlsI GCNGC 9 cut(s) 39, 239, 272, 275, 746, 969, 1534, 1962, 2209
BmcAI AGTACT 2 cut(s) 2491, 2514
Bme1390I CCNGG 6 cut(s) 289, 2298, 2528, 2714, 2962, 3005
Bme18I GGWCC 2 cut(s) 790, 3001
BmeRI GACNNNNNGTC 1 cut(s) 585
BmeT110I CYCGRG 1 cut(s) 1068
BmgBI CACGTC 1 cut(s) 440
BmgT120I GGNCC 5 cut(s) 235, 790, 1882, 1903, 3001
BmiI GGNNCC 6 cut(s) 85, 399, 505, 628, 792, 2523
BmrFI CCNGG 6 cut(s) 289, 2298, 2528, 2714, 2962, 3005
BmrI ACTGGG 1 cut(s) 2778
BmsI GCATC 8 cut(s) 419, 1212, 1234, 1445, 1955, 1975, 2164, 2358
BmtI GCTAGC 1 cut(s) 918
BmuI ACTGGG 1 cut(s) 2778
BpmI CTGGAG 2 cut(s) 2568, 2989
Bpu10I CCTNAGC 1 cut(s) 2214
Bpu14I TTCGAA 1 cut(s) 75
BpuEI CTTGAG 4 cut(s) 690, 1150, 1953, 2681
BpuMI CCSGG 1 cut(s) 289
BsaBI GATNNNNATC 3 cut(s) 76, 954, 2139
BsaHI GRCGYC 1 cut(s) 264
BsaI GGTCTC 2 cut(s) 115, 1065
BsaJI CCNNGG 9 cut(s) 8, 238, 688, 2034, 2296, 2526, 2538, 2713, 2818
BsaWI WCCGGW 2 cut(s) 80, 394
Bsc4I CCNNNNNNNGG 6 cut(s) 689, 927, 1082, 1312, 2398, 2967
Bse118I RCCGGY 2 cut(s) 394, 1051
Bse1I ACTGG 5 cut(s) 815, 1930, 2585, 2773, 2972
Bse21I CCTNAGG 1 cut(s) 126
Bse8I GATNNNNATC 3 cut(s) 76, 954, 2139
BseBI CCWGG 5 cut(s) 2298, 2528, 2714, 2962, 3005
BseDI CCNNGG 9 cut(s) 8, 238, 688, 2034, 2296, 2526, 2538, 2713, 2818
BseGI GGATG 5 cut(s) 284, 358, 1225, 1266, 2155
BseJI GATNNNNATC 3 cut(s) 76, 954, 2139
BseLI CCNNNNNNNGG 6 cut(s) 689, 927, 1082, 1312, 2398, 2967
BseMII CTCAG 6 cut(s) 117, 1323, 2013, 2205, 2223, 2570
BseNI ACTGG 5 cut(s) 815, 1930, 2585, 2773, 2972
BseXI GCAGC 6 cut(s) 49, 756, 979, 1544, 1947, 2219
BseYI CCCAGC 1 cut(s) 2333
BsgI GTGCAG 1 cut(s) 1551
Bsh1236I CGCG 2 cut(s) 240, 742
Bsh1285I CGRYCG 1 cut(s) 1189
BshFI GGCC 8 cut(s) 237, 723, 1051, 1884, 1904, 2361, 2581, 3008
BshNI GGYRCC 2 cut(s) 83, 397
BshTI ACCGGT 1 cut(s) 394
BsiEI CGRYCG 1 cut(s) 1189
BsiHKAI GWGCWC 2 cut(s) 1237, 2476
BsiHKCI CYCGRG 1 cut(s) 1068
BsiSI CCGG 6 cut(s) 44, 81, 288, 395, 407, 1052
BslFI GGGAC 4 cut(s) 45, 592, 2350, 2466
BslI CCNNNNNNNGG 6 cut(s) 689, 927, 1082, 1312, 2398, 2967
BsmAI GTCTC 4 cut(s) 115, 1065, 1671, 1971
BsmBI CGTCTC 1 cut(s) 1971
BsmFI GGGAC 4 cut(s) 45, 592, 2350, 2466
BsmI GAATGC 2 cut(s) 2785, 2883
BsnI GGCC 8 cut(s) 237, 723, 1051, 1884, 1904, 2361, 2581, 3008
Bso31I GGTCTC 2 cut(s) 115, 1065
BsoBI CYCGRG 1 cut(s) 1068
Bsp119I TTCGAA 1 cut(s) 75
Bsp1286I GDGCHC 3 cut(s) 164, 1237, 2476
BspANI GGCC 8 cut(s) 237, 723, 1051, 1884, 1904, 2361, 2581, 3008
BspCNI CTCAG 6 cut(s) 118, 1324, 2012, 2206, 2224, 2569
BspFNI CGCG 2 cut(s) 240, 742
BspLI GGNNCC 6 cut(s) 85, 399, 505, 628, 792, 2523
BspMAI CTGCAG 1 cut(s) 2209
BspMI ACCTGC 2 cut(s) 267, 2872
BspOI GCTAGC 1 cut(s) 918
BspPI GGATC 4 cut(s) 621, 634, 898, 1858
BspQI GCTCTTC 1 cut(s) 1731
BspT104I TTCGAA 1 cut(s) 75
BspT107I GGYRCC 2 cut(s) 83, 397
BspTNI GGTCTC 2 cut(s) 115, 1065
BsrFI RCCGGY 2 cut(s) 394, 1051
BsrI ACTGG 5 cut(s) 815, 1930, 2585, 2773, 2972
BssAI RCCGGY 2 cut(s) 394, 1051
BssECI CCNNGG 9 cut(s) 8, 238, 688, 2034, 2296, 2526, 2538, 2713, 2818
BssNAI GTATAC 1 cut(s) 1860
BssNI GRCGYC 1 cut(s) 264
BssSI CACGAG 2 cut(s) 17, 1230
BssT1I CCWWGG 4 cut(s) 8, 688, 2034, 2538
Bst1107I GTATAC 1 cut(s) 1860
Bst2BI CACGAG 2 cut(s) 17, 1230
Bst2UI CCWGG 5 cut(s) 2298, 2528, 2714, 2962, 3005
Bst4CI ACNGT 7 cut(s) 658, 697, 1282, 1614, 2494, 2819, 2891
Bst6I CTCTTC 3 cut(s) 1731, 1821, 2120
BstACI GRCGYC 1 cut(s) 264
BstAPI GCANNNNNTGC 2 cut(s) 973, 2471
BstBI TTCGAA 1 cut(s) 75
BstC8I GCNNGC 6 cut(s) 164, 227, 916, 1475, 2724, 2841
BstDEI CTNAG 8 cut(s) 126, 1332, 1999, 2214, 2232, 2556, 2672, 2938
BstDSI CCRYGG 2 cut(s) 238, 2818
BstEII GGTNACC 2 cut(s) 291, 2039
BstF5I GGATG 5 cut(s) 284, 358, 1225, 1266, 2155
BstFNI CGCG 2 cut(s) 240, 742
BstHHI GCGC 2 cut(s) 744, 1362
BstMAI GTCTC 4 cut(s) 115, 1065, 1671, 1971
BstMCI CGRYCG 1 cut(s) 1189
BstMWI GCNNNNNNNGC 8 cut(s) 246, 270, 750, 967, 973, 1366, 2471, 2663
BstNI CCWGG 5 cut(s) 2298, 2528, 2714, 2962, 3005
BstNSI RCATGY 2 cut(s) 1477, 2411
BstPI GGTNACC 2 cut(s) 291, 2039
BstSCI CCNGG 6 cut(s) 287, 2296, 2526, 2712, 2960, 3003
BstSFI CTRYAG 1 cut(s) 2205
BstUI CGCG 2 cut(s) 240, 742
BstV1I GCAGC 6 cut(s) 49, 756, 979, 1544, 1947, 2219
BstX2I RGATCY 4 cut(s) 626, 890, 1289, 1850
BstXI CCANNNNNNTGG 1 cut(s) 2051
BstYI RGATCY 4 cut(s) 626, 890, 1289, 1850
BstZ17I GTATAC 1 cut(s) 1860
Bsu36I CCTNAGG 1 cut(s) 126
BsuI GTATCC 1 cut(s) 1760
BsuRI GGCC 8 cut(s) 237, 723, 1051, 1884, 1904, 2361, 2581, 3008
BtgI CCRYGG 2 cut(s) 238, 2818
BtgZI GCGATG 1 cut(s) 1503
BtrI CACGTC 1 cut(s) 440
BtsCI GGATG 5 cut(s) 284, 358, 1225, 1266, 2155
BtsIMutI CAGTG 5 cut(s) 822, 1619, 2502, 2766, 2896
BveI ACCTGC 2 cut(s) 267, 2872
Cac8I GCNNGC 6 cut(s) 164, 227, 916, 1475, 2724, 2841
CaiI CAGNNNCTG 3 cut(s) 2213, 2967, 3004
CfoI GCGC 2 cut(s) 744, 1362
Cfr10I RCCGGY 2 cut(s) 394, 1051
Cfr13I GGNCC 5 cut(s) 235, 790, 1882, 1903, 3001
Cfr42I CCGCGG 1 cut(s) 241
CseI GACGC 2 cut(s) 253, 1479
Csp6I GTAC 7 cut(s) 134, 398, 693, 1296, 2252, 2490, 2513
CspAI ACCGGT 1 cut(s) 394
CviQI GTAC 7 cut(s) 134, 398, 693, 1296, 2252, 2490, 2513
DdeI CTNAG 8 cut(s) 126, 1332, 1999, 2214, 2232, 2556, 2672, 2938
DraIII CACNNNGTG 1 cut(s) 1219
DriI GACNNNNNGTC 1 cut(s) 585
EaeI YGGCCR 3 cut(s) 721, 2359, 3006
Eam1104I CTCTTC 3 cut(s) 1731, 1821, 2120
Eam1105I GACNNNNNGTC 1 cut(s) 585
EarI CTCTTC 3 cut(s) 1731, 1821, 2120
EciI GGCGGA 1 cut(s) 257
Eco130I CCWWGG 4 cut(s) 8, 688, 2034, 2538
Eco24I GRGCYC 1 cut(s) 164
Eco31I GGTCTC 2 cut(s) 115, 1065
Eco47I GGWCC 2 cut(s) 790, 3001
Eco57I CTGAAG 2 cut(s) 764, 1616
Eco81I CCTNAGG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 1068
Eco91I GGTNACC 2 cut(s) 291, 2039
EcoO109I RGGNCCY 1 cut(s) 3001
EcoO65I GGTNACC 2 cut(s) 291, 2039
EcoRI GAATTC 1 cut(s) 941
EcoRII CCWGG 5 cut(s) 2296, 2526, 2712, 2960, 3003
EcoT14I CCWWGG 4 cut(s) 8, 688, 2034, 2538
EcoT22I ATGCAT 6 cut(s) 1039, 1227, 1438, 1479, 1647, 2413
EcoT38I GRGCYC 1 cut(s) 164
ErhI CCWWGG 4 cut(s) 8, 688, 2034, 2538
Esp3I CGTCTC 1 cut(s) 1971
FalI AAGNNNNNCTT 2 cut(s) 762, 794
FaqI GGGAC 4 cut(s) 45, 592, 2350, 2466
FauI CCCGC 1 cut(s) 171
FauNDI CATATG 2 cut(s) 603, 972
FbaI TGATCA 3 cut(s) 1204, 1270, 2728
FblI GTMKAC 1 cut(s) 1859
Fnu4HI GCNGC 9 cut(s) 38, 238, 271, 274, 745, 968, 1533, 1961, 2208
FokI GGATG 5 cut(s) 271, 365, 1212, 1273, 2162
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 9 cut(s) 38, 238, 271, 274, 745, 968, 1533, 1961, 2208
FspBI CTAG 8 cut(s) 9, 915, 1125, 1293, 1761, 1794, 1866, 2144
GlaI GCGC 2 cut(s) 743, 1361
GluI GCNGC 9 cut(s) 38, 238, 271, 274, 745, 968, 1533, 1961, 2208
GsaI CCCAGC 1 cut(s) 2337
GsuI CTGGAG 2 cut(s) 2568, 2989
HaeIII GGCC 8 cut(s) 237, 723, 1051, 1884, 1904, 2361, 2581, 3008
HapII CCGG 6 cut(s) 44, 81, 288, 395, 407, 1052
HgaI GACGC 2 cut(s) 253, 1479
HhaI GCGC 2 cut(s) 744, 1362
Hin1I GRCGYC 1 cut(s) 264
Hin6I GCGC 2 cut(s) 742, 1360
HinP1I GCGC 2 cut(s) 742, 1360
HincII GTYRAC 3 cut(s) 282, 2086, 2887
HindII GTYRAC 3 cut(s) 282, 2086, 2887
HindIII AAGCTT 3 cut(s) 1682, 2365, 2547
HpaII CCGG 6 cut(s) 44, 81, 288, 395, 407, 1052
Hpy166II GTNNAC 6 cut(s) 282, 572, 1860, 2086, 2155, 2887
Hpy8I GTNNAC 6 cut(s) 282, 572, 1860, 2086, 2155, 2887
HpyAV CCTTC 8 cut(s) 830, 987, 1485, 2272, 2368, 2554, 2632, 2983
HpyCH4III ACNGT 7 cut(s) 658, 697, 1282, 1614, 2494, 2819, 2891
HpyCH4IV ACGT 4 cut(s) 58, 439, 757, 2846
HpyF10VI GCNNNNNNNGC 8 cut(s) 246, 270, 750, 967, 973, 1366, 2471, 2663
HpyF3I CTNAG 8 cut(s) 126, 1332, 1999, 2214, 2232, 2556, 2672, 2938
HpySE526I ACGT 4 cut(s) 58, 439, 757, 2846
Hsp92I GRCGYC 1 cut(s) 264
HspAI GCGC 2 cut(s) 742, 1360
KpnI GGTACC 1 cut(s) 401
Ksp22I TGATCA 3 cut(s) 1204, 1270, 2728
KspI CCGCGG 1 cut(s) 241
LguI GCTCTTC 1 cut(s) 1731
LmnI GCTCC 8 cut(s) 509, 665, 710, 923, 1308, 2215, 2899, 3019
Lsp1109I GCAGC 6 cut(s) 49, 756, 979, 1544, 1947, 2219
LweI GCATC 8 cut(s) 419, 1212, 1234, 1445, 1955, 1975, 2164, 2358
MaeI CTAG 8 cut(s) 9, 915, 1125, 1293, 1761, 1794, 1866, 2144
MaeII ACGT 4 cut(s) 58, 439, 757, 2846
MaeIII GTNAC 7 cut(s) 291, 327, 650, 817, 2039, 2073, 2401
MfeI CAATTG 1 cut(s) 2246
MflI RGATCY 4 cut(s) 626, 890, 1289, 1850
MhlI GDGCHC 3 cut(s) 164, 1237, 2476
MlsI TGGCCA 2 cut(s) 723, 3008
MluNI TGGCCA 2 cut(s) 723, 3008
MlyI GAGTC 1 cut(s) 1967
Mox20I TGGCCA 2 cut(s) 723, 3008
Mph1103I ATGCAT 6 cut(s) 1039, 1227, 1438, 1479, 1647, 2413
MroXI GAANNNNTTC 3 cut(s) 777, 941, 2378
MscI TGGCCA 2 cut(s) 723, 3008
MslI CAYNNNNRTG 4 cut(s) 180, 438, 1527, 2160
Msp20I TGGCCA 2 cut(s) 723, 3008
MspA1I CMGCKG 2 cut(s) 240, 563
MspI CCGG 6 cut(s) 44, 81, 288, 395, 407, 1052
MspR9I CCNGG 6 cut(s) 289, 2298, 2528, 2714, 2962, 3005
MunI CAATTG 1 cut(s) 2246
Mva1269I GAATGC 2 cut(s) 2785, 2883
MvaI CCWGG 5 cut(s) 2298, 2528, 2714, 2962, 3005
MvnI CGCG 2 cut(s) 240, 742
MwoI GCNNNNNNNGC 8 cut(s) 246, 270, 750, 967, 973, 1366, 2471, 2663
NciI CCSGG 1 cut(s) 289
NdeI CATATG 2 cut(s) 603, 972
NheI GCTAGC 1 cut(s) 914
NlaIV GGNNCC 6 cut(s) 85, 399, 505, 628, 792, 2523
NmuCI GTSAC 6 cut(s) 291, 327, 650, 817, 2039, 2073
NsiI ATGCAT 6 cut(s) 1039, 1227, 1438, 1479, 1647, 2413
NspI RCATGY 2 cut(s) 1477, 2411
NspV TTCGAA 1 cut(s) 75
OliI CACNNNNGTG 2 cut(s) 438, 2160
PaeI GCATGC 1 cut(s) 1477
PaeR7I CTCGAG 1 cut(s) 1068
PciSI GCTCTTC 1 cut(s) 1731
PctI GAATGC 2 cut(s) 2785, 2883
PdmI GAANNNNTTC 3 cut(s) 777, 941, 2378
PflFI GACNNNGTC 1 cut(s) 1614
PflMI CCANNNNNTGG 3 cut(s) 689, 2398, 2967
PinAI ACCGGT 1 cut(s) 394
PkrI GCNGC 9 cut(s) 39, 239, 272, 275, 746, 969, 1534, 1962, 2209
Ple19I CGATCG 1 cut(s) 1189
PleI GAGTC 1 cut(s) 1967
PpsI GAGTC 1 cut(s) 1967
PpuMI RGGWCCY 1 cut(s) 3001
PshBI ATTAAT 1 cut(s) 1032
PsiI TTATAA 2 cut(s) 701, 2013
Psp5II RGGWCCY 1 cut(s) 3001
Psp6I CCWGG 5 cut(s) 2296, 2526, 2712, 2960, 3003
PspEI GGTNACC 2 cut(s) 291, 2039
PspFI CCCAGC 1 cut(s) 2333
PspGI CCWGG 5 cut(s) 2296, 2526, 2712, 2960, 3003
PspN4I GGNNCC 6 cut(s) 85, 399, 505, 628, 792, 2523
PspPI GGNCC 5 cut(s) 235, 790, 1882, 1903, 3001
PspPPI RGGWCCY 1 cut(s) 3001
PsrI GAACNNNNNNTAC 2 cut(s) 2505, 2537
PstI CTGCAG 1 cut(s) 2209
PstNI CAGNNNCTG 3 cut(s) 2213, 2967, 3004
PsuI RGATCY 4 cut(s) 626, 890, 1289, 1850
PsyI GACNNNGTC 1 cut(s) 1614
PvuI CGATCG 1 cut(s) 1189
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 7 cut(s) 135, 399, 694, 1297, 2253, 2491, 2514
RsaNI GTAC 7 cut(s) 134, 398, 693, 1296, 2252, 2490, 2513
RseI CAYNNNNRTG 4 cut(s) 180, 438, 1527, 2160
SacII CCGCGG 1 cut(s) 241
SapI GCTCTTC 1 cut(s) 1731
SatI GCNGC 9 cut(s) 38, 238, 271, 274, 745, 968, 1533, 1961, 2208
Sau96I GGNCC 5 cut(s) 235, 790, 1882, 1903, 3001
ScaI AGTACT 2 cut(s) 2491, 2514
SchI GAGTC 1 cut(s) 1967
ScrFI CCNGG 6 cut(s) 289, 2298, 2528, 2714, 2962, 3005
SduI GDGCHC 3 cut(s) 164, 1237, 2476
SfaNI GCATC 8 cut(s) 419, 1212, 1234, 1445, 1955, 1975, 2164, 2358
SfcI CTRYAG 1 cut(s) 2205
Sfr274I CTCGAG 1 cut(s) 1068
Sfr303I CCGCGG 1 cut(s) 241
SfuI TTCGAA 1 cut(s) 75
SgrBI CCGCGG 1 cut(s) 241
SinI GGWCC 2 cut(s) 790, 3001
SlaI CTCGAG 1 cut(s) 1068
SmiMI CAYNNNNRTG 4 cut(s) 180, 438, 1527, 2160
SmlI CTYRAG 5 cut(s) 705, 1068, 1165, 1968, 2660
SmoI CTYRAG 5 cut(s) 705, 1068, 1165, 1968, 2660
SphI GCATGC 1 cut(s) 1477
SspI AATATT 2 cut(s) 145, 2605
SspMI CTAG 8 cut(s) 9, 915, 1125, 1293, 1761, 1794, 1866, 2144
StyD4I CCNGG 6 cut(s) 287, 2296, 2526, 2712, 2960, 3003
StyI CCWWGG 4 cut(s) 8, 688, 2034, 2538
TaaI ACNGT 7 cut(s) 658, 697, 1282, 1614, 2494, 2819, 2891
TaiI ACGT 4 cut(s) 61, 442, 760, 2849
TaqI TCGA 3 cut(s) 75, 1069, 2372
TatI WGTACW 2 cut(s) 2489, 2512
TauI GCSGC 3 cut(s) 240, 273, 276
TscAI CASTG 5 cut(s) 822, 1619, 2509, 2773, 2896
TseFI GTSAC 6 cut(s) 291, 327, 650, 817, 2039, 2073
TseI GCWGC 6 cut(s) 37, 744, 967, 1532, 1960, 2207
Tsp45I GTSAC 6 cut(s) 291, 327, 650, 817, 2039, 2073
TspGWI ACGGA 1 cut(s) 2871
TspRI CASTG 5 cut(s) 822, 1619, 2509, 2773, 2896
Tth111I GACNNNGTC 1 cut(s) 1614
Van91I CCANNNNNTGG 3 cut(s) 689, 2398, 2967
VpaK11BI GGWCC 2 cut(s) 790, 3001
VspI ATTAAT 1 cut(s) 1032
XapI RAATTY 5 cut(s) 941, 989, 1348, 1497, 1940
XbaI TCTAGA 3 cut(s) 1793, 1865, 2143
XceI RCATGY 2 cut(s) 1477, 2411
XhoI CTCGAG 1 cut(s) 1068
XmaJI CCTAGG 1 cut(s) 8
XmiI GTMKAC 1 cut(s) 1859
XmnI GAANNNNTTC 3 cut(s) 777, 941, 2378
XspI CTAG 8 cut(s) 9, 915, 1125, 1293, 1761, 1794, 1866, 2144
ZrmI AGTACT 2 cut(s) 2491, 2514
Zsp2I ATGCAT 6 cut(s) 1039, 1227, 1438, 1479, 1647, 2413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.