RchiOBHm_Chr1g0348711

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005282
Physical Location & Seq
Forward (+)
41677398 .. 41696238
18841 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 7203 bp
ATGCTCAAGTTTTCGCAGGCCACTGCGAACCAGATTCGGTTCCTCCTGCAAAGCCTCAATGACGCCAACTCGGACACTGTTCACCGAGAGCTTACTCAGTTTATTGAATATGGAGTTGAGGGAAGCGTTTTGCTGCTCCAAACCTGCCTGGATCATTTGAATCATTACAGAACAGATACAAAGAATGTGGCACTGGAACGGGTTCTTGCCTCAATTTTCAAATATCTCATCGACCAACCAAATTTCAGTACTATGTTTTGTGAATCACTAAGAAATACAGAGGTCAATGAAGGAATTGTGGAGAATTTCTCGAGTGTATTGCAACTGTCTGTACCTGAGAAAATTGGCATTGGTCTTGCTTTGTCAGATTCAGAAAACCTAGACACCAGAATATGTGGCAAAAACTTCTGTATGGCTCAGATTGAGAAATTGTGTGAAACTCCTGTTGACAGGATCTCTTCTGAGAAAATTCAGAATATTGTTATGTTCCTTCAGCGGTCTGAGGGTCTTTCCAAGCATGTAGATTCGTTTATGCAGATATTATCTTTACTGCAGCTGAAAGATGATGAAAACTCTTTTGTTTTAAGTCCATTGCTTTCAGATGAGCTTCGTGATGCCAATTTTTTGAGGAACATGGACTTGTTCCGTGAAAGTGGAGAAAATGATTTTGACGCTATTTTAGCAGAAATGGAGAAGGAGATGAATATGGGAGATATTATGAAGGAATTAGGTTATGGATGCACAGTTGATTCATCTCAGTGCAAAGAGATTTTATCCCTTTTCTTACCCCTCAATGAGATTACTATCTCCAAAATACTTGGCACGATTGCCTGCACCCATGCTGGTCTTGAGGATAATCAGAATACATTTTCTACCTTCCGGTTGGCGCTTGGTTATAGTACTTCATCTGATTTGCCTATGCTAAACACCTGGAACATTGATGTCCTTGTGGATATGATCAATCAACTTGCTCCAGACACCAATTGGATAAGTGTTATTGAAAATTTGGATCATGAGGGGTTCTACATTCCTAATCAGGAGGCATTCTCTTTTTTCATGTCTGTATATCAGCATGTGTGTCAGGAGCCATTCCCCCTACATGCCATCTGTGGGTCTGTTTGGAAGAATACTGAGGGTCAGCTATCTTTTCTCAAACATGCTGTTTCATCACCACCTGAAGTATTTAATTTTGCTCACTCTGTTAGGCAGCTGCCCTATATGGATACGGTGAATGGCCACAAGCTACAACTTGGACATGCAAATCATGCATGGTTGTGTCTAGACCTTTTGGATGTACTTTGTCACCTAGCAGAGAGGGGTCATGCTCTTTCTGTTCGATCGATACTTGAGTATCCTCTTCAACACTGTCCTGAAGTCCTGCTTCTTGGGATGGCACACATTAATACTCCATACAACCTGCTGCAATATGAAGTGTCTTTCATTGTTTTCCCCATGATAGTAAAAAATGTCATGGGTAATGGCATGATTGTTGACCTCTGGCATACTAACCCCTCTTTGGTGCTGCGGGGATTTGTGGATGCCTACAATAGCGATCCAGACAGCATGACTAGAATATTGGATATCTGTCAAGAGCTTAAGATTTTGTCGTCCGTCATGGAGATGATTCCTTCTCCCTTTAGTATCAGACTGGCGGCCCTTGCTTTCAGAAAAGAGCTTGTAGAACTCGAGAAGTGGTTGAGTAGTAATTTGAATACATATAAAGATACTTTCTTTGAGGAGTGCCTCAAGTTCCTGAAGGAGATTCAAGCTGGAGGGACCCAAGATTTCTCAACCAGACCTTTCCAACATGCTGGTGCTGTCTCAACTCTCTATGGGGACACTACTGCTACATTCTCGAAGGTTCTTAAAGCTCATGTCGCCCAGATTACTTCTAGCCACCTTACTGAGGAGATGGAAAAACTCTCTCTATCAATTATGGATTCTAATCCAAGGCTGCAGAATGGTGGGCCTACAGAGTCATCAACAGATGGATATGCAGATGACATTGAAGCTGAAGCCAACTCTTACTTCCATCAAATGTTTTCTGGTCAGTTGACTATTGAATCAATGGTCCAAATGCTTGCCCGTTTCAAAGAATCTTCTGTTCAAAGGGAACAATCCATTTTTGAGTGCATGATTGCTAATCTGTTTGAAGAGTACAGATTCTTTCCGAAATATCCTGAAAGACAGCTCAAAATTGCTGCAATTTTATTTGGGTCTGTCATCAAGCAGCAGCTTGTAACTCATCTGACTCTAGGGATTGCTCTGCGTGGTGTTTTAGATGCATTGCGCAAACCTGCGGATTCAAAAATGTTTGTGTTTGGAACAAAGGCTCTGGAGCAGTTTGTGGAGCGTCTGATTGAATGGCCACAGTACTGCAATCACATTCTTCAGATATCCCATTTGCGCAATACTCATTCAGAGCTGGTGGTATTCATTGAACAGGCTCTTGCCAGGATTTCATCTGGTCATTCAGATTCAGATGGAAGTAACCATGCTTCTGCCGTTCATCATCACGGTCCCAGTCAAGTTACCTCAGGAAATGTGGAGTTAAATGGATCAAGCATCTTACAAACTGGGCAGACACTATCTGCTCCGCTCCAGCTCCATCAGAGACATGAAAGCTCTTTTGATGACCGTCACAAGTCCTCAACTCCATCTAATGAGGTAAAGCCACTTGTATCTTCTGTGACGCAACCGGCAGTTGTTCCTGTTAGTGACGCTCCGAGTATTCAGAAGTTACAAAATGCAGTTAGTGCTCCATCAATGTTGTCTTCTGCTTCCCCTGGTTTTGTTCGTCCGTCACGAGGAGCTACCTCTACTAGGTTTGGCTCTGCTTTGAGCATTGAAACATTGGTTGCAGCAGCTGAGAAAAGGGATGCTCCCGTTGAGGCTCCAGCAGAGATTCAGGACAAAGTATTATTTATAGTTAATAATATATCACTTGCTAATATTGAGGTTAAGTCGAAGGAGTTTTCTGAGATACTGAAAGAGCAATACTATCCCTGGTTTGCGCAGTATATGGTTATGAAAAGGGCAAGCATTGAGCCCAATTTTCATGACTTGTACCTGAAATTTCTGGACAAACTTAATTCGAAGGCTTTGAATAAAGAGATTGTTCAAGCCACGTATGAGAACTGCAAGGTTCTTCTAGGGTCTGAGCTTATAAAATCAAGCTCAGAGGAGCGTTCATTGCTAAAAAATTTAGGTAGCTGGCTTGGGAAGCTTACTATTGGAAGGAATCAAGTCTTAAGGGCTCGTGAAATTGATCCCAAATCTTTGATCATAGAGGCATATGAGAAAGGGTTGATGATTGCTGTCATACCTTTTACTTCAAAGATTTTGGAACCATGTCAGAATAGCCTCGCATATCAACCACCGAATCCCTGGACAATGGGTATTCTTGGATTACTTGCTGAGATCTATTCCATGCCAAACTTGAAGATGAACCTCAAGTTTGATATAGAGGTTCTATTCAAGAATCTAGGAGTGGATTTGAAGGAGATAACACCAACTTCTCTTCTCAAGGATCGCAACAGAGAACTTGAAGGGAATCCAGATTTCTCCAACAAAGATGTTGGAGCATCTCAACCACAGATTGCTGAAGTTAAATCTGGAATTATTTCTCCACTAAACCAAGTTGAGCTACCACTTGACGTTGCTCCATCTAATTCTGGGAGCCACACACATCTGTTATCTCAGTATGCTACCTTGATGGAGGATGACAAGCTTGCTTTGGGGTTATCTGATCAGATCACTTCTGCTCAAGGACTGTTACAAGCTACTCCATCACAGTCCCCATTTTCTGTTAGTCAGCATCCCACAGCAATACCTAACATTGGAACCCACGTAATTATCAACCAGAAGCTCAGTGCCTTGGGGGGGCTGCACGTGCATTTCCAAAGAGTGGTTCCAATTGCTATGGATAGAGCTATCAAAGAAATTGTCTCTGGTATTGTTCAGCGCAGTGTTTCTATAGCTACCCAGACAACTAAGGAGCTTGTTTTAAAGGACTATGCCATGGAATCAGATGACACAAGAATTTTTACTGCAGCGCACTTGATGGTCGCCAGTTTGGCAGGAAGTCTAGCTCATGTCACATGCAAGGAACCCTTGCGCAGTTCAATATTAAGTCAGCTAAGAAATTCTCTTCAGGGATTAAACATTGCAAGTGATCTTCTAGAACAAGCTGTTCAACTTGTAACCAATGATAATCTGGATCTTGGCTGTGCAGTCATTGAGCAGGCTGCTACAGATAAGGCGGTAGCAACAATTGACGGGGAAATAGGTCCGCAGCTTTCAGTAAGAAGAAAGAGAGAAGGTGTTGGTGCAACCTATTTTGATGCAAACATGTATCCACAAGGTTCTATGGGTGTTGTGCCAGAAGCTCTCCGCCCTAAACCTGGTCATTTGTCCCTTTCTCAGCAACGAGTTTATGAGGATTTTGTTCGGCTTCCCTGGCAAAACCAATCTATTCAAAGTTCGCACGTTCTGCCAGTTGCTACCACAGCTTCATCTGGTAGTACTGGTCTCACTAGCACATATGGTTCAGCATCCGGACAACTTAACACGGGCTACTCACCAGGACCTGGAAGCAAATTTGAAGCTGTTTCTCGTCCATTAGATGAAGCGATGGAATCCAATTCTGCCCTCCATCTTAGTGCTCCATCTATTCATGTTGGGATGGCTGATGGTGTTTCCCAGCATAGTTCTGAAAATGATTCTGTTGTTGCCTCATTTCCTTCTGCTGCTTCTGCCCCTGAGCTGCACTCTGTAGAGTCATCTGATGCTGTAAAGGAATCTGGAGTGTCCTCACAGCCACCACCTTCACCTGCTGTAACTGAACGTCTTGGAAGTCTCTCAGAACCTTCCCTCACCACAGGGGCTGCATTGGAAAAATACCAGATTGTTGCACAGAAGTTGGAAGCTCTGGTGACTAACGATGCTAGAGAATCAGAAATCCAGGGAGTCATTGGTGAAGTACCTGATATTATACTCAGGTGTGTTAGTCGAGATGAGGCTGCCTTGGCTGTGGCTCAAAAGGTTTTCAAGGGTTTGTACGAGAATGCAACAAACCAGATTCATGTTGGCGCACATCTAGCAATGCTGACTGCGATTCGTGATGTTTGCAAGCTTGTCTTTACGCAGCTCACTAGTTGGGTGATTTATTCTGATGAGGAGCGGAAGTTTAATAAAGAAATTACTGTTGGCCTTATACGCAGTGAACTGCTAAACCTTGCAGAGTACAATGTTCACATGGCAAAACTTATTGATGGTGGCAGGAATAAGCCAGCAACAGAGTTCGCCACTTCCTTGCTCCAAACTTTGGTTATTGAAGAATCTAAAGTTATCTCAGAACTTCAGAGTCTTGTTGATGCAATGGGAAAGGCTGCTGCGAAGCCTGGATCTCCCGAGTCACTGCAGCAGGTGGTTGAAATTGTCAAGAATCCTGCTGCTAATGGAGCTGCTCCATCTGGTGTTAATGTTGGGAAGGATGACAAGGCCAGACAATCGAGAGAGAAAAAGGCTTCTGTTCACTCCCCAGTAAGCAGGGAAGAGTTCAGTATTGTGGAGTCTGCGGAACCAGATCCTCCTGGTTTTCGCGAGCAGGTGTCTATGCTGTTTGCTGAGTGGTACCGGATTTGTGAACTTCCTGGTGCAAACGAAAATAGTTGTGCCCATTTAATCATACAGTTGCACTCAAGTGGCATGCTGAATGGGGATGATCTTACGGACCGATTTTTCCGGCTACTCACAGAGCTTACTGTTGCACATTGTCTATCTTCTGAGGTGATTAATTCAGGCACACTGCAAACTCCTCAACAAGTGCAGAATCTATCCTTCCTTGCCATTGATATTTATGCTAAACTTGTATTTTTAATTCTCAAGATGGAGCCGGGATCAAGTAAACTAATGCTCTTGTCCAAGATTTTGGCAGTTACAGTGAGATTTATTCAAAAGGATGCAGAGGAGAAGAAATCATCTTTCAATGCAAGACCGTATTTTAGATTGTTTGTTAACTGGCTGCTAGACCTTGGGTCTTTGGATCCTGTTATTGATGGTGCTAACTTTCAGATATTGACAGCCTTCGCAAATGCATTTCATGCGTTGCAACCCCTCAAAGTTCCTACATTCAGCTTTGCATGGCTCGAGTTGGTGAGTCATAGGAGTTTCATGCCTAAAATGCTAGCTGGAAATGGTCAGAAGGGTTGGCCCTATATCCAACGTTTGCTGGTTGACTTATTTCAATTCATGGAGCCTTTTTTGAGGAATGCTGAACTAGGAGTTCCGGTTCACTTCCTGTATAAAGGCACCCTTAGAGTGTTACTTGTGCTGCTCCATGATTTCCCTGAGTTCCTTTGTGATTATCACTTCACTTTCTGTGATGTTATTCCCCCGAGTTGCATTCAAATGCGAAATATCATCCTCAGTGCATTTCCCCGTAACATGAGGCTTCCTGACCCATCTACTCCAAACTTAAAGATTGATTTGCTTGCTGAAATCACTCAATCTCCACGTGTTCTTTCTGAGGTTGATGCAGCTCTGAAATTAAAGCAGATGAAGACCGATGTGGATGAGTATCTCAAGACAAGGCAACAAGGTTCTTCATTTTTGACCGAATTGAAGCAAAAATTGCTCCTTTTACCAACCGAAGCTGCCTCAGCTGGTACTCGTTACAATGTACCCCTCATCAACTCCCTTGTACTCTATGTGGGGATGCAGGCTATCCAGCAGCTTCAGGCTAGAACACCTCACGCACAGGCAAATCAAACTGTTCCATTTGTATATTTGGTGGGTGCTGCTTTGGATATCTTCCAGACTCTGATAGTGGACCTAGATACTGAAGGACGCTACCTTTTCCTAAATGCAATTGCAAACCAACTGCGCTATCCGAACACCCATACACATTACTTCTCCTTCATTGTTCTTTACTTGTTCGCGGAGTCAAACCAGGAAATTATCCAGGAGCAAATCACAAGGGTATTGTTGGAACGTCTCATTGTTAATCGCCCTCACCCATGGGGTCTACTGATAACATTCATTGAGCTAATCAAGAATCCGAGGTACAACTTCTGGAACCGGGCTTTCATAAGGTGTGCACCAGAGATTGAAAAACTCTTTGAATCTGTATCCAGGTCTTGTGGCGCAAAGCCTGTGGATGAGAGTATGGTTTCAGGTTGGGTACCTGAGACTGCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

2400

Amino Acids

266.31

Weight (kDa)

5.54

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNOT1_HEAT PF16418 482 - 623 2.5e-33 CCR4-NOT transcription complex subunit 1 HEAT repeat
CNOT1_TTP_bind PF16417 658 - 820 2.5e-67 CCR4-NOT transcription complex subunit 1 TTP binding domain
CNOT1_CAF1_bind PF16415 964 - 1181 6e-90 CCR4-NOT transcription complex subunit 1 CAF1-binding domain
DUF3819 PF12842 1299 - 1438 8.1e-48 CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819
ARM_Cnot1 PF25097 1640 - 1816 3.4e-53 Cnot1 ARM repeat
NOT1_connector PF23590 1664 - 1814 8.1e-16 CCR4-NOT transcription complex subunit 1, NOT1_connector
Not1 PF04054 2018 - 2377 6e-142 CCR4-Not complex component, Not1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3167
AarI CACCTGC 3 cut(s) 4840, 5416, 5599
Acc16I TGCGCA 4 cut(s) 2291, 2408, 3015, 4118
Acc36I ACCTGC 6 cut(s) 152, 1425, 2305, 4840, 5416, 5599
Acc65I GGTACC 2 cut(s) 5634, 7183
AccB1I GGYRCC 3 cut(s) 5634, 6311, 7183
AccB7I CCANNNNNTGG 3 cut(s) 3907, 4589, 5326
AccBSI CCGCTC 2 cut(s) 2599, 5182
AccI GTMKAC 1 cut(s) 7027
AccII CGCG 2 cut(s) 5604, 6941
AccIII TCCGGA 1 cut(s) 4556
AclI AACGTT 1 cut(s) 6226
AcoI YGGCCR 2 cut(s) 1234, 2366
AcsI RAATTY 9 cut(s) 241, 304, 468, 1003, 3074, 3202, 4041, 4144, 4598
AcvI CACGTG 2 cut(s) 3892, 6518
AcyI GRCGYC 1 cut(s) 63
AflII CTTAAG 2 cut(s) 1595, 3250
AflIII ACRYGT 2 cut(s) 4350, 6517
AhdI GACNNNNNGTC 1 cut(s) 6037
AhlI ACTAGT 1 cut(s) 5153
AjuI GAANNNNNNNTTGG 6 cut(s) 2012, 2044, 3894, 3926, 5190, 5222
AleI CACNNNNGTG 1 cut(s) 5703
AloI GAACNNNNNNTCC 2 cut(s) 6276, 6308
Alw21I GWGCWC 3 cut(s) 2761, 4666, 7102
Alw26I GTCTC 7 cut(s) 1825, 2608, 3952, 4535, 4862, 7001, 7184
Alw44I GTGCAC 1 cut(s) 7098
AlwNI CAGNNNCTG 4 cut(s) 2867, 4589, 4886, 6823
Ama87I CYCGRG 5 cut(s) 310, 1685, 5411, 6149, 6397
Aor13HI TCCGGA 1 cut(s) 4556
AoxI GGCC 8 cut(s) 18, 1234, 1653, 1967, 2366, 5209, 5502, 6212
ApaLI GTGCAC 1 cut(s) 7098
ApoI RAATTY 9 cut(s) 241, 304, 468, 1003, 3074, 3202, 4041, 4144, 4598
ArsI GACNNNNNNTTYG 2 cut(s) 3930, 3962
AseI ATTAAT 2 cut(s) 1401, 5796
Asp700I GAANNNNTTC 2 cut(s) 201, 3622
Asp718I GGTACC 2 cut(s) 5634, 7183
AsuC2I CCSGG 2 cut(s) 5898, 7082
AsuII TTCGAA 1 cut(s) 3095
AsuNHI GCTAGC 1 cut(s) 6187
AvaI CYCGRG 5 cut(s) 310, 1685, 5411, 6149, 6397
AvaII GGWCC 7 cut(s) 1776, 2071, 2519, 4289, 4586, 5734, 6832
AxyI CCTNAGG 1 cut(s) 2536
BaeGI GKGCMC 2 cut(s) 5680, 7102
BaeI ACNNNNGTAYC 4 cut(s) 1334, 1334, 1367, 1367
BalI TGGCCA 2 cut(s) 1236, 2368
BamHI GGATCC 1 cut(s) 6046
BanI GGYRCC 3 cut(s) 5634, 6311, 7183
BanII GRGCYC 2 cut(s) 3051, 3259
BarI GAAGNNNNNNTAC 2 cut(s) 2482, 2514
BauI CACGAG 2 cut(s) 2805, 3258
BbrPI CACGTG 2 cut(s) 3892, 6518
BbsI GAAGAC 2 cut(s) 2766, 6569
Bbv12I GWGCWC 3 cut(s) 2761, 4666, 7102
BbvCI CCTCAGC 1 cut(s) 6661
BceAI ACGGC 1 cut(s) 2489
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BciVI GTATCC 4 cut(s) 1216, 1362, 4365, 7141
BclI TGATCA 3 cut(s) 957, 3282, 3748
BcnI CCSGG 2 cut(s) 5898, 7082
BcoDI GTCTC 7 cut(s) 1825, 2608, 3952, 4535, 4862, 7001, 7184
BcuI ACTAGT 1 cut(s) 5153
BfmI CTRYAG 8 cut(s) 551, 1955, 1971, 3975, 4050, 4251, 4773, 5420
BfoI RGCGCY 1 cut(s) 890
BfrI CTTAAG 2 cut(s) 1595, 3250
BfuAI ACCTGC 6 cut(s) 152, 1425, 2305, 4840, 5416, 5599
BfuI GTATCC 4 cut(s) 1216, 1362, 4365, 7141
BglI GCCNNNNNGGC 1 cut(s) 4076
BglII AGATCT 1 cut(s) 3420
BmcAI AGTACT 4 cut(s) 250, 901, 2375, 4525
Bme18I GGWCC 7 cut(s) 1776, 2071, 2519, 4289, 4586, 5734, 6832
BmeRI GACNNNNNGTC 1 cut(s) 6037
BmeT110I CYCGRG 5 cut(s) 310, 1685, 5411, 6149, 6397
BmrI ACTGGG 3 cut(s) 2517, 2586, 5537
BmtI GCTAGC 1 cut(s) 6191
BmuI ACTGGG 3 cut(s) 2517, 2586, 5537
BpiI GAAGAC 2 cut(s) 2766, 6569
BplI GAGNNNNNCTC 6 cut(s) 293, 325, 1031, 1063, 4754, 4786
BpmI CTGGAG 6 cut(s) 957, 1791, 2357, 2585, 2880, 4824
Bpu10I CCTNAGC 2 cut(s) 4761, 6661
Bpu14I TTCGAA 1 cut(s) 3095
BpuEI CTTGAG 9 cut(s) 869, 1367, 1730, 3437, 3509, 3750, 5686, 5870, 6569
BpuMI CCSGG 2 cut(s) 5898, 7082
Bsa29I ATCGAT 1 cut(s) 1340
BsaAI YACGTR 4 cut(s) 3129, 3850, 3892, 6518
BsaBI GATNNNNATC 3 cut(s) 803, 1944, 6579
BsaHI GRCGYC 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 4535
BsaWI WCCGGW 4 cut(s) 879, 4556, 5637, 6289
BsaXI ACNNNNNCTCC 6 cut(s) 5171, 5201, 6276, 6306, 6496, 6526
Bse118I RCCGGY 1 cut(s) 2698
Bse1I ACTGG 9 cut(s) 198, 1653, 2523, 2581, 4071, 4496, 4531, 5543, 6026
Bse21I CCTNAGG 1 cut(s) 2536
Bse3DI GCAATG 6 cut(s) 590, 2285, 3191, 4164, 5109, 5385
Bse8I GATNNNNATC 3 cut(s) 803, 1944, 6579
BseAI TCCGGA 1 cut(s) 4556
BseCI ATCGAT 1 cut(s) 1340
BseJI GATNNNNATC 3 cut(s) 803, 1944, 6579
BseMI GCAATG 6 cut(s) 590, 2285, 3191, 4164, 5109, 5385
BseNI ACTGG 9 cut(s) 198, 1653, 2523, 2581, 4071, 4496, 4531, 5543, 6026
BseRI GAGGAG 7 cut(s) 1751, 1922, 2823, 3197, 5192, 5808, 5984
BseSI GKGCMC 2 cut(s) 5680, 7102
BseYI CCCAGC 1 cut(s) 4701
BsgI GTGCAG 5 cut(s) 817, 3872, 4251, 4751, 5849
Bsh1236I CGCG 2 cut(s) 5604, 6941
Bsh1285I CGRYCG 1 cut(s) 1340
BshFI GGCC 8 cut(s) 20, 1236, 1655, 1969, 2368, 5211, 5504, 6214
BshNI GGYRCC 3 cut(s) 5634, 6311, 7183
BshVI ATCGAT 1 cut(s) 1340
BsiEI CGRYCG 1 cut(s) 1340
BsiHKAI GWGCWC 3 cut(s) 2761, 4666, 7102
BsiHKCI CYCGRG 5 cut(s) 310, 1685, 5411, 6149, 6397
BsiSI CCGG 8 cut(s) 880, 2699, 4557, 5638, 5746, 5897, 6290, 7081
BslFI GGGAC 5 cut(s) 1789, 1850, 2505, 3781, 4399
BsmAI GTCTC 7 cut(s) 1825, 2608, 3952, 4535, 4862, 7001, 7184
BsmBI CGTCTC 1 cut(s) 7001
BsmFI GGGAC 5 cut(s) 1789, 1850, 2505, 3781, 4399
BsmI GAATGC 4 cut(s) 1043, 5071, 6277, 6405
BsnI GGCC 8 cut(s) 20, 1236, 1655, 1969, 2368, 5211, 5504, 6214
Bso31I GGTCTC 1 cut(s) 4535
BsoBI CYCGRG 5 cut(s) 310, 1685, 5411, 6149, 6397
Bsp119I TTCGAA 1 cut(s) 3095
Bsp1286I GDGCHC 6 cut(s) 2761, 3051, 3259, 4666, 5680, 7102
Bsp13I TCCGGA 1 cut(s) 4556
Bsp19I CCATGG 2 cut(s) 4020, 7019
Bsp68I TCGCGA 1 cut(s) 5604
BspANI GGCC 8 cut(s) 20, 1236, 1655, 1969, 2368, 5211, 5504, 6214
BspDI ATCGAT 1 cut(s) 1340
BspEI TCCGGA 1 cut(s) 4556
BspFNI CGCG 2 cut(s) 5604, 6941
BspHI TCATGA 2 cut(s) 1012, 3058
BspMAI CTGCAG 4 cut(s) 555, 1959, 4054, 5424
BspMI ACCTGC 6 cut(s) 152, 1425, 2305, 4840, 5416, 5599
BspOI GCTAGC 1 cut(s) 6191
BspT104I TTCGAA 1 cut(s) 3095
BspT107I GGYRCC 3 cut(s) 5634, 6311, 7183
BspTI CTTAAG 2 cut(s) 1595, 3250
BspTNI GGTCTC 1 cut(s) 4535
BsrBI CCGCTC 2 cut(s) 2599, 5182
BsrDI GCAATG 6 cut(s) 590, 2285, 3191, 4164, 5109, 5385
BsrFI RCCGGY 1 cut(s) 2698
BsrI ACTGG 9 cut(s) 198, 1653, 2523, 2581, 4071, 4496, 4531, 5543, 6026
BssAI RCCGGY 1 cut(s) 2698
BssNI GRCGYC 1 cut(s) 63
BssSI CACGAG 2 cut(s) 2805, 3258
BssT1I CCWWGG 6 cut(s) 1949, 3876, 4020, 5025, 6034, 7019
Bst2BI CACGAG 2 cut(s) 2805, 3258
Bst6I CTCTTC 6 cut(s) 463, 1362, 2148, 3525, 4155, 5550
BstACI GRCGYC 1 cut(s) 63
BstAFI CTTAAG 2 cut(s) 1595, 3250
BstAPI GCANNNNNTGC 5 cut(s) 1265, 2756, 4492, 6104, 6634
BstBAI YACGTR 4 cut(s) 3129, 3850, 3892, 6518
BstBI TTCGAA 1 cut(s) 3095
BstDSI CCRYGG 2 cut(s) 4020, 7019
BstENI CCTNNNNNAGG 2 cut(s) 1904, 2821
BstFNI CGCG 2 cut(s) 5604, 6941
BstH2I RGCGCY 1 cut(s) 890
BstMAI GTCTC 7 cut(s) 1825, 2608, 3952, 4535, 4862, 7001, 7184
BstMCI CGRYCG 1 cut(s) 1340
BstNSI RCATGY 9 cut(s) 521, 1076, 1103, 1160, 1259, 1811, 4104, 4354, 5713
BstSFI CTRYAG 8 cut(s) 551, 1955, 1971, 3975, 4050, 4251, 4773, 5420
BstSLI GKGCMC 2 cut(s) 5680, 7102
BstUI CGCG 2 cut(s) 5604, 6941
BstV2I GAAGAC 2 cut(s) 2766, 6569
BstX2I RGATCY 6 cut(s) 453, 3420, 4219, 5405, 5587, 6046
BstXI CCANNNNNNTGG 2 cut(s) 1811, 5932
BstYI RGATCY 6 cut(s) 453, 3420, 4219, 5405, 5587, 6046
Bsu15I ATCGAT 1 cut(s) 1340
Bsu36I CCTNAGG 1 cut(s) 2536
BsuI GTATCC 4 cut(s) 1216, 1362, 4365, 7141
BsuRI GGCC 8 cut(s) 20, 1236, 1655, 1969, 2368, 5211, 5504, 6214
BsuTUI ATCGAT 1 cut(s) 1340
BtgI CCRYGG 2 cut(s) 4020, 7019
BtgZI GCGATG 1 cut(s) 4646
BtsI GCAGTG 5 cut(s) 21, 3973, 5227, 5417, 5807
BtuMI TCGCGA 1 cut(s) 5604
BveI ACCTGC 6 cut(s) 152, 1425, 2305, 4840, 5416, 5599
CaiI CAGNNNCTG 4 cut(s) 2867, 4589, 4886, 6823
CciI TCATGA 2 cut(s) 1012, 3058
Cfr10I RCCGGY 1 cut(s) 2698
ClaI ATCGAT 1 cut(s) 1340
CpoI CGGWCCG 1 cut(s) 5734
CseI GACGC 6 cut(s) 71, 680, 2342, 2701, 2729, 6858
CsiI ACCWGGT 1 cut(s) 4402
CspCI CAANNNNNGTGG 4 cut(s) 168, 203, 7137, 7172
CspI CGGWCCG 1 cut(s) 5734
DraI TTTAAA 1 cut(s) 4008
DriI GACNNNNNGTC 1 cut(s) 6037
EaeI YGGCCR 2 cut(s) 1234, 2366
Eam1104I CTCTTC 6 cut(s) 463, 1362, 2148, 3525, 4155, 5550
Eam1105I GACNNNNNGTC 1 cut(s) 6037
EarI CTCTTC 6 cut(s) 463, 1362, 2148, 3525, 4155, 5550
EciI GGCGGA 1 cut(s) 4382
Eco130I CCWWGG 6 cut(s) 1949, 3876, 4020, 5025, 6034, 7019
Eco24I GRGCYC 2 cut(s) 3051, 3259
Eco31I GGTCTC 1 cut(s) 4535
Eco32I GATATC 3 cut(s) 1582, 2397, 6811
Eco47I GGWCC 7 cut(s) 1776, 2071, 2519, 4289, 4586, 5734, 6832
Eco72I CACGTG 2 cut(s) 3892, 6518
Eco81I CCTNAGG 1 cut(s) 2536
Eco88I CYCGRG 5 cut(s) 310, 1685, 5411, 6149, 6397
EcoNI CCTNNNNNAGG 2 cut(s) 1904, 2821
EcoO109I RGGNCCY 2 cut(s) 1776, 4586
EcoRV GATATC 3 cut(s) 1582, 2397, 6811
EcoT14I CCWWGG 6 cut(s) 1949, 3876, 4020, 5025, 6034, 7019
EcoT22I ATGCAT 3 cut(s) 1270, 2287, 6100
EcoT38I GRGCYC 2 cut(s) 3051, 3259
ErhI CCWWGG 6 cut(s) 1949, 3876, 4020, 5025, 6034, 7019
Esp3I CGTCTC 1 cut(s) 7001
FalI AAGNNNNNCTT 8 cut(s) 1365, 1397, 4097, 4129, 5123, 5155, 6300, 6332
FaqI GGGAC 5 cut(s) 1789, 1850, 2505, 3781, 4399
FauI CCCGC 1 cut(s) 1518
FauNDI CATATG 2 cut(s) 3295, 4543
FbaI TGATCA 3 cut(s) 957, 3282, 3748
FblI GTMKAC 1 cut(s) 7027
FriOI GRGCYC 2 cut(s) 3051, 3259
FspI TGCGCA 4 cut(s) 2291, 2408, 3015, 4118
GsaI CCCAGC 1 cut(s) 4705
GsuI CTGGAG 6 cut(s) 957, 1791, 2357, 2585, 2880, 4824
HaeII RGCGCY 1 cut(s) 890
HaeIII GGCC 8 cut(s) 20, 1236, 1655, 1969, 2368, 5211, 5504, 6214
HapII CCGG 8 cut(s) 880, 2699, 4557, 5638, 5746, 5897, 6290, 7081
HgaI GACGC 6 cut(s) 71, 680, 2342, 2701, 2729, 6858
Hin1I GRCGYC 1 cut(s) 63
HincII GTYRAC 5 cut(s) 448, 1492, 2055, 6019, 6238
HindII GTYRAC 5 cut(s) 448, 1492, 2055, 6019, 6238
HindIII AAGCTT 3 cut(s) 3224, 3728, 5132
HpaI GTTAAC 1 cut(s) 6019
HpaII CCGG 8 cut(s) 880, 2699, 4557, 5638, 5746, 5897, 6290, 7081
HpyCH4IV ACGT 9 cut(s) 3128, 3657, 3849, 3891, 4488, 4846, 6226, 6517, 6994
HpySE526I ACGT 9 cut(s) 3128, 3657, 3849, 3891, 4488, 4846, 6226, 6517, 6994
Hsp92I GRCGYC 1 cut(s) 63
KflI GGGWCCC 1 cut(s) 1776
Kpn2I TCCGGA 1 cut(s) 4556
KpnI GGTACC 2 cut(s) 5638, 7187
Ksp22I TGATCA 3 cut(s) 957, 3282, 3748
KspAI GTTAAC 1 cut(s) 6019
MabI ACCWGGT 1 cut(s) 4402
MaeII ACGT 9 cut(s) 3128, 3657, 3849, 3891, 4488, 4846, 6226, 6517, 6994
MbiI CCGCTC 2 cut(s) 2599, 5182
MfeI CAATTG 4 cut(s) 982, 3915, 4272, 6870
MflI RGATCY 6 cut(s) 453, 3420, 4219, 5405, 5587, 6046
MhlI GDGCHC 6 cut(s) 2761, 3051, 3259, 4666, 5680, 7102
MlsI TGGCCA 2 cut(s) 1236, 2368
MluNI TGGCCA 2 cut(s) 1236, 2368
MmeI TCCRAC 6 cut(s) 1828, 3559, 3591, 4902, 6247, 6969
Mox20I TGGCCA 2 cut(s) 1236, 2368
Mph1103I ATGCAT 3 cut(s) 1270, 2287, 6100
MroI TCCGGA 1 cut(s) 4556
MroXI GAANNNNTTC 2 cut(s) 201, 3622
MscI TGGCCA 2 cut(s) 1236, 2368
MslI CAYNNNNRTG 7 cut(s) 757, 1273, 1619, 1812, 4659, 5703, 6410
Msp20I TGGCCA 2 cut(s) 1236, 2368
MspA1I CMGCKG 5 cut(s) 496, 556, 1210, 2867, 6665
MspCI CTTAAG 2 cut(s) 1595, 3250
MspI CCGG 8 cut(s) 880, 2699, 4557, 5638, 5746, 5897, 6290, 7081
MunI CAATTG 4 cut(s) 982, 3915, 4272, 6870
Mva1269I GAATGC 4 cut(s) 1043, 5071, 6277, 6405
MvnI CGCG 2 cut(s) 5604, 6941
NciI CCSGG 2 cut(s) 5898, 7082
NcoI CCATGG 2 cut(s) 4020, 7019
NdeI CATATG 2 cut(s) 3295, 4543
NheI GCTAGC 1 cut(s) 6187
NmuCI GTSAC 8 cut(s) 1301, 2639, 2689, 2717, 2802, 4096, 4933, 5415
NruI TCGCGA 1 cut(s) 5604
NsbI TGCGCA 4 cut(s) 2291, 2408, 3015, 4118
NsiI ATGCAT 3 cut(s) 1270, 2287, 6100
NspI RCATGY 9 cut(s) 521, 1076, 1103, 1160, 1259, 1811, 4104, 4354, 5713
NspV TTCGAA 1 cut(s) 3095
OliI CACNNNNGTG 1 cut(s) 5703
PaeI GCATGC 1 cut(s) 5713
PaeR7I CTCGAG 3 cut(s) 310, 1685, 6149
PagI TCATGA 2 cut(s) 1012, 3058
PaqCI CACCTGC 3 cut(s) 4840, 5416, 5599
PciI ACATGT 1 cut(s) 4350
PcsI WCGNNNNNNNCGW 1 cut(s) 2803
PctI GAATGC 4 cut(s) 1043, 5071, 6277, 6405
PdmI GAANNNNTTC 2 cut(s) 201, 3622
PflMI CCANNNNNTGG 3 cut(s) 3907, 4589, 5326
PfoI TCCNGGA 1 cut(s) 6963
Ple19I CGATCG 1 cut(s) 1340
PmaCI CACGTG 2 cut(s) 3892, 6518
PmlI CACGTG 2 cut(s) 3892, 6518
Ppu21I YACGTR 4 cut(s) 3129, 3850, 3892, 6518
PpuMI RGGWCCY 2 cut(s) 1776, 4586
PscI ACATGT 1 cut(s) 4350
PshBI ATTAAT 2 cut(s) 1401, 5796
PsiI TTATAA 1 cut(s) 3167
Psp1406I AACGTT 1 cut(s) 6226
Psp5II RGGWCCY 2 cut(s) 1776, 4586
PspCI CACGTG 2 cut(s) 3892, 6518
PspFI CCCAGC 1 cut(s) 4701
PspPPI RGGWCCY 2 cut(s) 1776, 4586
PspXI VCTCGAGB 1 cut(s) 6149
PstI CTGCAG 4 cut(s) 555, 1959, 4054, 5424
PstNI CAGNNNCTG 4 cut(s) 2867, 4589, 4886, 6823
PsuI RGATCY 6 cut(s) 453, 3420, 4219, 5405, 5587, 6046
PvuI CGATCG 1 cut(s) 1340
PvuII CAGCTG 4 cut(s) 556, 1210, 2867, 6665
RruI TCGCGA 1 cut(s) 5604
RseI CAYNNNNRTG 7 cut(s) 757, 1273, 1619, 1812, 4659, 5703, 6410
Rsr2I CGGWCCG 1 cut(s) 5734
RsrII CGGWCCG 1 cut(s) 5734
ScaI AGTACT 4 cut(s) 250, 901, 2375, 4525
SduI GDGCHC 6 cut(s) 2761, 3051, 3259, 4666, 5680, 7102
SexAI ACCWGGT 1 cut(s) 4402
SfcI CTRYAG 8 cut(s) 551, 1955, 1971, 3975, 4050, 4251, 4773, 5420
Sfr274I CTCGAG 3 cut(s) 310, 1685, 6149
SfuI TTCGAA 1 cut(s) 3095
SinI GGWCC 7 cut(s) 1776, 2071, 2519, 4289, 4586, 5734, 6832
SlaI CTCGAG 3 cut(s) 310, 1685, 6149
SmiMI CAYNNNNRTG 7 cut(s) 757, 1273, 1619, 1812, 4659, 5703, 6410
SpeI ACTAGT 1 cut(s) 5153
SphI GCATGC 1 cut(s) 5713
SspI AATATT 4 cut(s) 478, 1575, 2953, 4128
StyI CCWWGG 6 cut(s) 1949, 3876, 4020, 5025, 6034, 7019
TaiI ACGT 9 cut(s) 3131, 3660, 3852, 3894, 4491, 4849, 6229, 6520, 6997
TaqII GACCGA 3 cut(s) 5751, 6581, 6632
TatI WGTACW 8 cut(s) 248, 899, 1294, 2157, 2373, 4523, 5244, 6703
TauI GCSGC 1 cut(s) 1655
TseFI GTSAC 8 cut(s) 1301, 2639, 2689, 2717, 2802, 4096, 4933, 5415
Tsp45I GTSAC 8 cut(s) 1301, 2639, 2689, 2717, 2802, 4096, 4933, 5415
TspGWI ACGGA 4 cut(s) 635, 1600, 2790, 5747
Van91I CCANNNNNTGG 3 cut(s) 3907, 4589, 5326
Vha464I CTTAAG 2 cut(s) 1595, 3250
VneI GTGCAC 1 cut(s) 7098
VpaK11BI GGWCC 7 cut(s) 1776, 2071, 2519, 4289, 4586, 5734, 6832
VspI ATTAAT 2 cut(s) 1401, 5796
XagI CCTNNNNNAGG 2 cut(s) 1904, 2821
XapI RAATTY 9 cut(s) 241, 304, 468, 1003, 3074, 3202, 4041, 4144, 4598
XbaI TCTAGA 2 cut(s) 1279, 4180
XceI RCATGY 9 cut(s) 521, 1076, 1103, 1160, 1259, 1811, 4104, 4354, 5713
XcmI CCANNNNNNNNNTGG 5 cut(s) 981, 3384, 4213, 4970, 5084
XhoI CTCGAG 3 cut(s) 310, 1685, 6149
XmiI GTMKAC 1 cut(s) 7027
XmnI GAANNNNTTC 2 cut(s) 201, 3622
ZrmI AGTACT 4 cut(s) 250, 901, 2375, 4525
Zsp2I ATGCAT 3 cut(s) 1270, 2287, 6100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.