RchiOBHm_Chr1g0379491

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0007019
Physical Location & Seq
Forward (+)
65514254 .. 65516461
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 816 bp
ATGGCAGAGCCAGAAGAGATACCAGGGGCGTCGCTCATGGAGAAGATCTACTCTCACCACCGCTCCTCGTCGTCTTCGTCGTCTTCCGACGACGACAAGCCGTCCAAGAAGGCCGATGCCGTCAAGCCCGATGACGTCAAGCACAAGGCTGAGGAACACGACATGCCGTCCGCTCACGATTCCGTCAAGTCCAACAAATTCAGGATCTTCGGCCGGGAAAAGCCCGTTCATAAGGTCCTCGGCGGTGGCAAACCTGCTGATGTCCTCCTATGGAGGGACAAGAAAGTATCTGGAAGTGTTCTTGGTGGAGCCACTGTACTATGGATTTTCTTTGAGTTGCTTGAATACCATTTGATAACTCTGGTCTGCCATATGTTGATACTTTCTCTGGGCGTTTTCTTCCTGTGGTCCAATGTGTCCTCCTTTATCAACAAGGCTCCACCAGAAATCCCAAAAGTTCAAATTCCGGAGAAGTGTGTCCTTGATCTTGCCTCTGCACTGAGGATTGAACTCAACAAAGGGTTCCATGTTCTGCGGGAAATTGCATCTGGGAGAGATTTGAGGACATTCCTGATTGTAATAGTTGTCTTGTGGATTTTGTCAGTGATTGGCAAATGGTTCAACTTCCTGACCTTATTCTACTTATCCTTTGTCTTGCTGCACACGGTGCCAGTGATCTATGAGAAACATGATGTCCAGATTGATGCTTTTGCTGAGAAGGCATGGATTGAGATTAAGAAGCAGTATGCAGTGTTTGATGCGAATGTTTTGAGTAAGATACCCAAGGGTCCAGTGAAAGAGAAGAAGAAGGACTAG

Protein Analysis

271

Amino Acids

30.58

Weight (kDa)

8.84

Isoelectric Point (pI)

45.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 86 - 241 7e-58 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
Acc36I ACCTGC 1 cut(s) 262
AccB1I GGYRCC 1 cut(s) 667
AccBSI CCGCTC 2 cut(s) 63, 173
AccIII TCCGGA 1 cut(s) 466
AciI CCGC 4 cut(s) 61, 171, 243, 535
AclWI GGATC 1 cut(s) 212
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 2 cut(s) 197, 462
AcyI GRCGYC 2 cut(s) 29, 135
AdeI CACNNNGTG 1 cut(s) 667
AfaI GTAC 1 cut(s) 318
AfiI CCNNNNNNNGG 1 cut(s) 274
AgsI TTSAA 4 cut(s) 344, 461, 509, 622
AhdI GACNNNNNGTC 2 cut(s) 100, 166
AjnI CCWGG 1 cut(s) 22
AlwI GGATC 1 cut(s) 212
Aor13HI TCCGGA 1 cut(s) 466
AoxI GGCC 2 cut(s) 111, 211
ApeKI GCWGC 1 cut(s) 658
ApoI RAATTY 2 cut(s) 197, 462
AspS9I GGNCC 3 cut(s) 235, 408, 788
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 1 cut(s) 47
AvaII GGWCC 3 cut(s) 235, 408, 788
BanI GGYRCC 1 cut(s) 667
BbsI GAAGAC 2 cut(s) 66, 75
BbvCI CCTCAGC 1 cut(s) 150
BbvI GCAGC 1 cut(s) 645
BceAI ACGGC 3 cut(s) 85, 104, 151
BciT130I CCWGG 1 cut(s) 24
BcnI CCSGG 1 cut(s) 215
BfaI CTAG 1 cut(s) 814
BfuAI ACCTGC 1 cut(s) 262
BglII AGATCT 1 cut(s) 45
BisI GCNGC 1 cut(s) 659
BlsI GCNGC 1 cut(s) 660
Bme1390I CCNGG 2 cut(s) 24, 215
Bme18I GGWCC 3 cut(s) 235, 408, 788
BmeRI GACNNNNNGTC 2 cut(s) 100, 166
BmgT120I GGNCC 3 cut(s) 235, 408, 788
BmiI GGNNCC 5 cut(s) 310, 438, 524, 669, 789
BmrFI CCNGG 2 cut(s) 24, 215
BmsI GCATC 4 cut(s) 106, 554, 694, 748
BpiI GAAGAC 2 cut(s) 66, 75
Bpu10I CCTNAGC 1 cut(s) 150
BpuMI CCSGG 1 cut(s) 215
BsaHI GRCGYC 2 cut(s) 29, 135
BsaJI CCNNGG 3 cut(s) 23, 238, 783
BsaWI WCCGGW 1 cut(s) 466
BsaXI ACNNNNNCTCC 4 cut(s) 47, 77, 300, 330
Bsc4I CCNNNNNNNGG 1 cut(s) 274
Bse1I ACTGG 2 cut(s) 671, 791
BseAI TCCGGA 1 cut(s) 466
BseBI CCWGG 1 cut(s) 24
BseDI CCNNGG 3 cut(s) 23, 238, 783
BseLI CCNNNNNNNGG 1 cut(s) 274
BseMII CTCAG 3 cut(s) 141, 491, 705
BseNI ACTGG 2 cut(s) 671, 791
BseRI GAGGAG 1 cut(s) 55
BseX3I CGGCCG 1 cut(s) 211
BseXI GCAGC 1 cut(s) 645
BsgI GTGCAG 2 cut(s) 480, 644
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 2 cut(s) 113, 213
BshNI GGYRCC 1 cut(s) 667
BsiEI CGRYCG 1 cut(s) 214
BsiSI CCGG 2 cut(s) 214, 467
BslFI GGGAC 1 cut(s) 290
BslI CCNNNNNNNGG 1 cut(s) 274
BsmFI GGGAC 1 cut(s) 290
BsnI GGCC 2 cut(s) 113, 213
Bsp13I TCCGGA 1 cut(s) 466
Bsp143I GATC 4 cut(s) 45, 204, 484, 675
BspACI CCGC 4 cut(s) 61, 171, 243, 535
BspANI GGCC 2 cut(s) 113, 213
BspCNI CTCAG 3 cut(s) 142, 492, 706
BspEI TCCGGA 1 cut(s) 466
BspLI GGNNCC 5 cut(s) 310, 438, 524, 669, 789
BspMI ACCTGC 1 cut(s) 262
BspPI GGATC 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 667
BsrBI CCGCTC 2 cut(s) 63, 173
BsrI ACTGG 2 cut(s) 671, 791
BssECI CCNNGG 3 cut(s) 23, 238, 783
BssMI GATC 4 cut(s) 45, 204, 484, 675
BssNI GRCGYC 2 cut(s) 29, 135
BssT1I CCWWGG 1 cut(s) 783
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 2 cut(s) 316, 667
Bst6I CTCTTC 1 cut(s) 9
BstACI GRCGYC 2 cut(s) 29, 135
BstAPI GCANNNNNTGC 1 cut(s) 667
BstDEI CTNAG 3 cut(s) 150, 500, 714
BstKTI GATC 4 cut(s) 48, 207, 487, 678
BstMBI GATC 4 cut(s) 45, 204, 484, 675
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 2 cut(s) 667, 719
BstNI CCWGG 1 cut(s) 24
BstNSI RCATGY 1 cut(s) 166
BstSCI CCNGG 2 cut(s) 22, 213
BstV1I GCAGC 1 cut(s) 645
BstV2I GAAGAC 2 cut(s) 66, 75
BstX2I RGATCY 2 cut(s) 45, 204
BstYI RGATCY 2 cut(s) 45, 204
BstZI CGGCCG 1 cut(s) 211
BsuRI GGCC 2 cut(s) 113, 213
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 6 cut(s) 312, 497, 609, 678, 756, 798
BveI ACCTGC 1 cut(s) 262
Cfr13I GGNCC 3 cut(s) 235, 408, 788
CseI GACGC 1 cut(s) 18
Csp6I GTAC 1 cut(s) 317
CviAII CATG 5 cut(s) 37, 163, 527, 689, 723
CviJI RGCY 9 cut(s) 10, 100, 113, 127, 149, 213, 223, 311, 437
CviKI_1 RGCY 9 cut(s) 10, 100, 113, 127, 149, 213, 223, 311, 437
CviQI GTAC 1 cut(s) 317
DdeI CTNAG 3 cut(s) 150, 500, 714
DpnI GATC 4 cut(s) 47, 206, 486, 677
DpnII GATC 4 cut(s) 45, 204, 484, 675
DraIII CACNNNGTG 1 cut(s) 667
DriI GACNNNNNGTC 2 cut(s) 100, 166
EaeI YGGCCR 1 cut(s) 211
EagI CGGCCG 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 9
Eam1105I GACNNNNNGTC 2 cut(s) 100, 166
EarI CTCTTC 1 cut(s) 9
EclXI CGGCCG 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 783
Eco47I GGWCC 3 cut(s) 235, 408, 788
Eco52I CGGCCG 1 cut(s) 211
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 783
ErhI CCWWGG 1 cut(s) 783
FaeI CATG 5 cut(s) 40, 166, 530, 692, 726
FaqI GGGAC 1 cut(s) 290
FatI CATG 5 cut(s) 36, 162, 526, 688, 722
FauI CCCGC 1 cut(s) 528
FauNDI CATATG 1 cut(s) 372
Fnu4HI GCNGC 1 cut(s) 659
Fsp4HI GCNGC 1 cut(s) 659
FspBI CTAG 1 cut(s) 814
GluI GCNGC 1 cut(s) 659
HaeIII GGCC 2 cut(s) 113, 213
HapII CCGG 2 cut(s) 214, 467
HgaI GACGC 1 cut(s) 18
Hin1I GRCGYC 2 cut(s) 29, 135
Hin1II CATG 5 cut(s) 40, 166, 530, 692, 726
HinfI GANTC 1 cut(s) 179
HpaII CCGG 2 cut(s) 214, 467
HphI GGTGA 1 cut(s) 47
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 7 cut(s) 176, 202, 291, 467, 571, 628, 697
Hpy99I CGWCG 5 cut(s) 34, 73, 82, 92, 95
HpyAV CCTTC 3 cut(s) 103, 712, 802
HpyCH4III ACNGT 2 cut(s) 316, 667
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 4 cut(s) 497, 545, 661, 749
HpyF10VI GCNNNNNNNGC 2 cut(s) 667, 719
HpyF3I CTNAG 3 cut(s) 150, 500, 714
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 2 cut(s) 29, 135
Hsp92II CATG 5 cut(s) 40, 166, 530, 692, 726
Kpn2I TCCGGA 1 cut(s) 466
Kzo9I GATC 4 cut(s) 45, 204, 484, 675
LmnI GCTCC 3 cut(s) 68, 308, 442
Lsp1109I GCAGC 1 cut(s) 645
LweI GCATC 4 cut(s) 106, 554, 694, 748
MaeI CTAG 1 cut(s) 814
MaeII ACGT 1 cut(s) 135
MalI GATC 4 cut(s) 47, 206, 486, 677
MbiI CCGCTC 2 cut(s) 63, 173
MboI GATC 4 cut(s) 45, 204, 484, 675
MboII GAAGA 7 cut(s) 26, 55, 66, 75, 199, 391, 814
MflI RGATCY 2 cut(s) 45, 204
MluCI AATT 3 cut(s) 197, 462, 540
MmeI TCCRAC 2 cut(s) 111, 216
MnlI CCTC 9 cut(s) 76, 145, 248, 267, 275, 430, 495, 502, 555
MroI TCCGGA 1 cut(s) 466
MseI TTAA 1 cut(s) 735
MspI CCGG 2 cut(s) 214, 467
MspR9I CCNGG 2 cut(s) 24, 215
MvaI CCWGG 1 cut(s) 24
MwoI GCNNNNNNNGC 2 cut(s) 667, 719
NciI CCSGG 1 cut(s) 215
NdeI CATATG 1 cut(s) 372
NdeII GATC 4 cut(s) 45, 204, 484, 675
NlaIII CATG 5 cut(s) 40, 166, 530, 692, 726
NlaIV GGNNCC 5 cut(s) 310, 438, 524, 669, 789
NmeAIII GCCGAG 1 cut(s) 219
NspI RCATGY 1 cut(s) 166
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 77
PfeI GAWTC 1 cut(s) 179
PkrI GCNGC 1 cut(s) 660
PpuMI RGGWCCY 1 cut(s) 235
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 22
PspGI CCWGG 1 cut(s) 22
PspN4I GGNNCC 5 cut(s) 310, 438, 524, 669, 789
PspPI GGNCC 3 cut(s) 235, 408, 788
PspPPI RGGWCCY 1 cut(s) 235
PsuI RGATCY 2 cut(s) 45, 204
RsaI GTAC 1 cut(s) 318
RsaNI GTAC 1 cut(s) 317
SaqAI TTAA 1 cut(s) 735
SatI GCNGC 1 cut(s) 659
Sau3AI GATC 4 cut(s) 45, 204, 484, 675
Sau96I GGNCC 3 cut(s) 235, 408, 788
ScrFI CCNGG 2 cut(s) 24, 215
SetI ASST 4 cut(s) 138, 237, 256, 635
SfaNI GCATC 4 cut(s) 106, 554, 694, 748
SinI GGWCC 3 cut(s) 235, 408, 788
Sse9I AATT 3 cut(s) 197, 462, 540
SsiI CCGC 4 cut(s) 61, 171, 243, 535
SspMI CTAG 1 cut(s) 814
StyD4I CCNGG 2 cut(s) 22, 213
StyI CCWWGG 1 cut(s) 783
TaaI ACNGT 2 cut(s) 316, 667
TaiI ACGT 1 cut(s) 138
TasI AATT 3 cut(s) 197, 462, 540
TatI WGTACW 1 cut(s) 316
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 1 cut(s) 735
Tru9I TTAA 1 cut(s) 735
TscAI CASTG 6 cut(s) 319, 504, 609, 678, 756, 798
TseI GCWGC 1 cut(s) 658
TspDTI ATGAA 1 cut(s) 218
TspGWI ACGGA 1 cut(s) 172
TspRI CASTG 6 cut(s) 319, 504, 609, 678, 756, 798
VpaK11BI GGWCC 3 cut(s) 235, 408, 788
XapI RAATTY 2 cut(s) 197, 462
XceI RCATGY 1 cut(s) 166
XspI CTAG 1 cut(s) 814
ZraI GACGTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.