RCHIOBHM_CPG0502661

Proton-conducting membrane transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Pt
OG0004593
Physical Location & Seq
Reverse (-)
124474 .. 125097
624 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15732

Sequence Viewer

Length: 357 bp
ATGTATAACTCCCCAGGAATTTCAATTGCGCTTATATTCATCACTGTAGGAATTGGGTTCAAGCTTTCCCTAGCCCCTTCTCATCAATGGACTCCTGACGTATACGAAGGAATGCTACATAGTTGGTTCTCATCCTTCAGAGACTACGAGTGTAATAGGAGCATCCGTCGACAAAAGGATCACCCTAAGATGATCATCTCATGGCTATTGAGAACGAATCAAATCAGATGGTTCTATTTCTCAATCTTTCTGACTTGCTCCTACGGAACCGAGGTCGAAAAGATTGAGAAAAATCAGTCATTCACAACCACTGATGAAGGATTCCTCGAAAAGTTAAGGATTAGTAATCCTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 map00190 map01100
Pfam Domains
Protein Families
Proton_antipo_M

Protein Analysis

118

Amino Acids

13.96

Weight (kDa)

8.62

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 1 - 37 1.4e-06 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 102, 169
AclWI GGATC 1 cut(s) 186
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 121
AgsI TTSAA 2 cut(s) 24, 61
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 1 cut(s) 64
AluI AGCT 1 cut(s) 64
Alw26I GTCTC 1 cut(s) 135
AlwI GGATC 1 cut(s) 186
ApoI RAATTY 1 cut(s) 18
AspLEI GCGC 1 cut(s) 31
AsuHPI GGTGA 1 cut(s) 173
BarI GAAGNNNNNNTAC 2 cut(s) 99, 131
BccI CCATC 1 cut(s) 222
BciT130I CCWGG 1 cut(s) 15
BclI TGATCA 1 cut(s) 192
BcoDI GTCTC 1 cut(s) 135
BfaI CTAG 1 cut(s) 71
BfmI CTRYAG 1 cut(s) 45
Bme1390I CCNGG 1 cut(s) 15
BmiI GGNNCC 1 cut(s) 268
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 1 cut(s) 171
BsaBI GATNNNNATC 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 13, 270
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bse8I GATNNNNATC 1 cut(s) 194
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 2 cut(s) 13, 270
BseGI GGATG 2 cut(s) 131, 162
BseJI GATNNNNATC 1 cut(s) 194
BsmAI GTCTC 1 cut(s) 135
BsmI GAATGC 1 cut(s) 117
Bsp143I GATC 2 cut(s) 178, 192
BspLI GGNNCC 1 cut(s) 268
BspPI GGATC 1 cut(s) 186
BssECI CCNNGG 2 cut(s) 13, 270
BssMI GATC 2 cut(s) 178, 192
BssNAI GTATAC 1 cut(s) 103
Bst1107I GTATAC 1 cut(s) 103
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 1 cut(s) 46
BstDEI CTNAG 1 cut(s) 186
BstF5I GGATG 2 cut(s) 131, 162
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 2 cut(s) 181, 195
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 2 cut(s) 178, 192
BstNI CCWGG 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 45
BstZ17I GTATAC 1 cut(s) 103
BtsCI GGATG 2 cut(s) 131, 162
BtsIMutI CAGTG 2 cut(s) 42, 309
CfoI GCGC 1 cut(s) 31
CviAII CATG 1 cut(s) 201
CviJI RGCY 3 cut(s) 64, 74, 205
CviKI_1 RGCY 3 cut(s) 64, 74, 205
DdeI CTNAG 1 cut(s) 186
DpnI GATC 2 cut(s) 180, 194
DpnII GATC 2 cut(s) 178, 192
Eco57I CTGAAG 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 13
FaeI CATG 1 cut(s) 204
FaiI YATR 5 cut(s) 6, 35, 103, 120, 202
FatI CATG 1 cut(s) 200
FbaI TGATCA 1 cut(s) 192
FblI GTMKAC 2 cut(s) 102, 169
FokI GGATG 2 cut(s) 118, 149
FspBI CTAG 1 cut(s) 71
GlaI GCGC 1 cut(s) 30
HhaI GCGC 1 cut(s) 31
Hin1II CATG 1 cut(s) 204
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HincII GTYRAC 1 cut(s) 170
HindII GTYRAC 1 cut(s) 170
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 3 cut(s) 91, 217, 321
HphI GGTGA 1 cut(s) 173
Hpy166II GTNNAC 2 cut(s) 103, 170
Hpy188I TCNGA 3 cut(s) 140, 227, 252
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 2 cut(s) 103, 170
Hpy99I CGWCG 1 cut(s) 171
HpyAV CCTTC 4 cut(s) 87, 101, 145, 311
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4IV ACGT 1 cut(s) 99
HpyF3I CTNAG 1 cut(s) 186
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 1 cut(s) 204
HspAI GCGC 1 cut(s) 29
Ksp22I TGATCA 1 cut(s) 192
Kzo9I GATC 2 cut(s) 178, 192
LmnI GCTCC 2 cut(s) 159, 263
LpnPI CCDG 2 cut(s) 27, 108
LweI GCATC 1 cut(s) 171
MaeI CTAG 1 cut(s) 71
MaeII ACGT 1 cut(s) 99
MalI GATC 2 cut(s) 180, 194
MboI GATC 2 cut(s) 178, 192
MfeI CAATTG 1 cut(s) 24
MluCI AATT 3 cut(s) 18, 24, 51
MlyI GAGTC 1 cut(s) 85
MnlI CCTC 2 cut(s) 265, 335
MseI TTAA 1 cut(s) 335
MspR9I CCNGG 1 cut(s) 15
MunI CAATTG 1 cut(s) 24
Mva1269I GAATGC 1 cut(s) 117
MvaI CCWGG 1 cut(s) 15
NdeII GATC 2 cut(s) 178, 192
NlaIII CATG 1 cut(s) 204
NlaIV GGNNCC 1 cut(s) 268
PctI GAATGC 1 cut(s) 117
PfeI GAWTC 2 cut(s) 217, 321
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
Psp6I CCWGG 1 cut(s) 13
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 1 cut(s) 268
SalI GTCGAC 1 cut(s) 168
SaqAI TTAA 1 cut(s) 335
Sau3AI GATC 2 cut(s) 178, 192
SchI GAGTC 1 cut(s) 85
ScrFI CCNGG 1 cut(s) 15
SetI ASST 3 cut(s) 66, 102, 276
SfaNI GCATC 1 cut(s) 171
SfcI CTRYAG 1 cut(s) 45
Sse9I AATT 3 cut(s) 18, 24, 51
SspMI CTAG 1 cut(s) 71
StyD4I CCNGG 1 cut(s) 13
TaaI ACNGT 1 cut(s) 46
TaiI ACGT 1 cut(s) 102
TaqI TCGA 3 cut(s) 169, 276, 327
TasI AATT 3 cut(s) 18, 24, 51
TfiI GAWTC 2 cut(s) 217, 321
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TscAI CASTG 2 cut(s) 49, 316
TspDTI ATGAA 2 cut(s) 28, 330
TspGWI ACGGA 2 cut(s) 155, 279
TspRI CASTG 2 cut(s) 49, 316
XapI RAATTY 1 cut(s) 18
XmiI GTMKAC 2 cut(s) 102, 169
XspI CTAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.